1 pathological expression of the germ cell testicular tumors and their biology

... SW-CEACAM6" cells exhibited CEA and CEACAM6 ( data not shown ) expression all over their surfaces and at cell – cell borders, further demonstrating their lack of polarity ( Figure6B and F ) Thus, ... surface of cells, at cell – cell borders and on cell surfaces in contact with the underlying matrix support, consistent with a lack of cellular polarization and strikingly reminiscent of the distorted ... a blood and tissue tumor marker of epithelial malignancy, especially for tumors of the colon The correlation of elevated blood and tumor levels of CEA and CEACAM6 with so many human tumors has

Ngày tải lên: 01/11/2022, 09:46

... overview of the research problems, state the research questions, the scope of the study and the significances of the study Chapter II (Literature review) offers the definition of key concepts and ... schools The research subject of this paper is the perception of pre-service teachers of the 21st century skills training and their application into the practicum The population size of this thesis ... skills training and their application in their practicum 2.3 Research gap Despite the recognized advantages of the integration of the 21st century skills into real teaching contexts, there still

Ngày tải lên: 16/03/2021, 09:42

... paper examines the labour market findings of the negative income tax experiments of the 1970s, and their impact on the public policy debate in the United States Although both side of the debate use ... demise of progressive social reform The primary aim of the NIT experiments was to test the effects of a guaranteed income on the work effort of recipients, and thereby to get some indication of the ... poverty as defined by official statistics The smaller the guarantee level is, the smaller the work disincentive and the smaller the cost of the programme will be, but the effect on the poverty rate

Ngày tải lên: 19/10/2022, 03:19

... include things like the accuracy of the language, the appropriateness of the topics for the target audience, the quality of the exercises, and the overall design of the textbook The Checklist Method ... skills and sub-skills) of the new English 3 textbook; their perceptions of the challenges and advantages of using the new English 3 textbook and their suggestions to enhance effective teaching The ... evaluation of the content of ELT textbooks 172.4.2 The evaluation of the skills of ELT textbooks 19 2.4.3 The evaluation of the activities of ELT textbooks 19 2.4.4 2.4.4 The evaluation of the language

Ngày tải lên: 25/03/2024, 14:51

... include things like the accuracy of the language, the appropriateness of the topics for the target audience, the quality of the exercises, and the overall design of the textbook The Checklist Method ... skills and sub-skills) of the new English 3 textbook; their perceptions of the challenges and advantages of using the new English 3 textbook and their suggestions to enhance effective teaching The ... evaluation of the content of ELT textbooks 172.4.2 The evaluation of the skills of ELT textbooks 19 2.4.3 The evaluation of the activities of ELT textbooks 19 2.4.4 2.4.4 The evaluation of the language

Ngày tải lên: 04/08/2024, 09:18

... attachment of the cells, they were serum-starved for 12 h and then incubated in the presence of liraglutide (0, 10, 100, and 1000 nmol/l), and the cells were allowed to grow for 10 days There-after, the ... Research from the Ministry of Education, Science and Culture (16 K20212, HH) The grant played a fundamental role in the design of the study and collection, analysis, and interpretation of data and in ... interpretation of data RK, TI and YS prepared and examined the TMA microscopically, and RK and YS conducted the pathological analysis RK and HH have been involved in drafting the manuscript, and OWH,

Ngày tải lên: 23/07/2020, 02:34

... individual bands on blots was measured by ImageJ and the ratio of the target protein to the housekeeping protein GAPDH was calculated The relative expression data were presented when the ratio of the ... CD68 and CD44 Among ALDH1A3 positive cells, there were some GFAP positive cells likely reactive astrocytes or tumor cells due to their distinct morphology As the co-expression of ALDH1A3 with the ... between the expression of ALDH1A3 and STAT3α The association and the underlying regulatory mechan-ism of ALDH1A3 and STAT3 in GBM need to be fur-ther elucidated by using gene techniques in the future

Ngày tải lên: 06/08/2020, 05:42

... 28.3 and 71.7% of cases in the study cohort and 44.1 and 55.9% of cases in the validation cohort suggesting similar expression patterns across the dif-ferent arrays In the study cohort 30% of cases ... phospho-ERK The observed association of CNKSR1 expression and survival outcome suggests scaffolding proteins of the RAS-MAPK pathway may account, in part, for the observed heterogeneity of PDAC biology, ... associations (SEER, UMD, and NIH) Table 2 presents the demographics and tumor characteristics by CNKSR1 expression status The majority of cases were 65 years of age or older at the time of diagnosis (59%),

Ngày tải lên: 06/08/2020, 06:24

... siRNA in Panc1 and BxPc3 cells and stably by shRNA in Panc1 cells Expression analysis of CDH1 and Vimentin mRNA was performed and cell motility was tested in a migration assay Panc1 cells transfected ... experiments that decreased expression of Six1 induces a declined transcrip-tion of CDH1 in Panc1 and in BxPc3 cells Moreover, reduced expression of Six1 slightly affected the expression of vimentin in Panc1, ... Conclusively, we hypothe-sized that decreased expression of Six1 would also result in a reduced number of tumour cells with a CSC-phenotype We therefore analysed the expression of CD24 and CD44 since

Ngày tải lên: 19/09/2020, 21:41

... Lenalidomide treatment of MM cells affects the steady-state levels of CRBN and AGO2 We and others have found that the treatment of MM cells with IMiDs affected the steady-state levels of CRBN and its downstream ... (M-anti-CRBN) and mouse-anti- actin antibody (M-anti-Actin) b Comparison of CRBN expression in MM cell lines The intensity of the β-actin band was considered as 1.0 and the relative intensity of CRBN band ... apoptosis Furthermore, directly reducing cellular AGO2 levels produced cellular cytotox-icity regardless of whether they are IMiD-sensitive or -resistant MM cells Therefore, the expression of CRBN-downstream

Ngày tải lên: 21/09/2020, 10:01

... expression of the IL-6R and of theco-receptor gp130 However, we did not detect the IL-6R on the surface of colorectal cancer cells by flow cytometry (data not shown) Therefore, we assumed that either the ... phosphorylation) or 48 h (CEACAM expression), cells were lysed and the lysates analyzed for the phosphorylation of STAT3 and the expression of CEACAM in Western blots and scanned in the Odyssey near-infrared ... similar amount of positive cells, but the expression level was lower than in HT29p cells Again, HT29c cells Fig 4 Hyper-IL-6 strongly activates STAT3 and increases the expression of CEACAM5 and CEACAM6

Ngày tải lên: 22/09/2020, 22:56

... of HIN-1 compared with the ES2 cells We then tested whether a demethylating agent could reverse the methylation of the HIN-1 gene and then reactivate the expression of HIN-1 The methylation of ... b High expression of HIN-1 Note: The high HIN-1 expression was noted in the cytoplasm of the neoplastic cells (arrows) c Bar figures of the percentage of weak and strong HIN-1 protein expression ... Page of 12 Fig a The effect of 10 μM or 100 μM of 5-aza-2-dC on in vitro cell growth of ES2 and ES2TR160 cells by MTT assay b The percentages of G1 and G2 phases in both ES2 and ES2TR160 cells

Ngày tải lên: 22/09/2020, 23:53

... Pediatric cancer, Germ cell tumors, miRNA, Methylation, Stem cell Background Germ cell tumors (GCTs) include germinomas, comprised of testicular seminomas and ovarian dysgerminomas, and nonseminomas, ... high levels of expression and red indicates low levels of expression The mixed tumor that clustered with the teratomas rather than the other mixed tumors included components of teratoma and YST b ... including the miRNAs, miR-122, −126, −302a and -302b and the stem cell genes, LEFTY1 and LEFTY2 These changes were influenced largely by differences in the expression in YST vs tumors of other histologic

Ngày tải lên: 23/09/2020, 00:07

... producing the chemokine CCL5 [39,40] The memory population may be further divided by the expression or loss of expression of CCR7 [12] We and others have previously shown that the majority of T cells ... values of CCL5, and error bars one standard deviation Phenotype of CCL5+ T cells within the joint CD8+ T cells of the effector and memory compartments have been shown to express high levels of the ... their own recruitment We analysed the phenotype of the CCL5+CCR5+CD8 T cell population within the joint Several studies have proposed stages of differentiation for effector and memory CD8 T cells:...

Ngày tải lên: 09/08/2014, 07:20

... processing of the D melanogaster a-F1-ATPase transcript The expression of the a-F1-ATPase gene during development is coordinated with the expression of the nuclearencoded b-F1-ATPase gene and the mitochondrial-encoded ... footprinting of the 5¢ upstream region of the a-F1-ATPase gene and found that the combination of GAF and Adf-1 binding sites is present both in other Drosophila species (D yakuba and D pseudoobscura) and ... under the control of the actin 5C promoter The GAGA factor stimulated at least threefold the activity of the promoter in constructs )397/+86 and )146/+86, but had no effect on the activity of the...

Ngày tải lên: 07/03/2014, 16:20

... suggesting that the level of GFP–PrPC transgene expression was dependent on the colour of the background of the animal The fusion protein was found only in the NIL and not in the AL of black- and white-adapted ... physiological manipulation of the biosynthetic and secretory activity of the melanotrope cell In this study, we combined the unique properties of the melanotrope cell with the technique of stable Xenopus ... Discussion The aim of the present study was to investigate the intracellular fate of PrPC by examining for the first time its biosynthesis in the secretory pathway of neuroendocrine cells in vivo and the...

Ngày tải lên: 16/03/2014, 14:20

... cells The expression of rPhl p in insect cells was optimized by infecting Sf9 cells with different amounts of virus and by expression for various periods Aliquots of the culture supernatants and cell ... 1, the cDNA coding for the mature allergen was expressed in baculovirus-infected insect cells An expression strategy was chosen which led to the secretion of the recombinant allergen into the cell ... insect cell- expressed Phl p and, to a lower degree, in the nPhl p preparations Biochemical and biophysical characterization of insect -cell expressed Phl p The molecular mass of insect cell- expressed...

Ngày tải lên: 16/03/2014, 18:20

... from Hanes plots Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined by ... cleaves 364-bp upstream of the start codon of qorM, and DraIII, that cleaves 8-bp downstream of the stop codon of qorL After removing the 3¢ overhang and filling the 5¢ overhang of the plasmid with T4 ... indicated in Fig 4B The absence of Mo(V)signals reveals the g2-component of FeSII It is noted that the g-factors of the FeSI and II signals of the Qor proteins from the wild-type strain and from P putida...

Ngày tải lên: 17/03/2014, 10:20

... explain the lack of the effect of the transgene on the size of the latter depot [16,17]; this is also associated with the differential effect of the transgene on in situ fatty acid synthesis in the ... multilocular cells in white fat, the morphology of mitochondria and the mitochondrial content of the unilocular cells were affected by the transgene The morphometric study of subcutaneous white fat of the ... young but not adult mice, and they were absent from epididymal fat, regardless of either the age of the animals, or the genotype Therefore, the results document further that the resistance against...

Ngày tải lên: 31/03/2014, 15:20

... RAGE expression in FLS cultures in our experiments and may be relevant to the over -expression of RAGE on RA synovial tissues However, the exact mechanism of RAGE over -expression in the milieu of ... pathway of IL-17-induced RAGE production and expression, we tested the effect of Act-1 shRNA on RAGE production We produced Act-1 shRNA and confirmed the inhibitory effect of Act-1 shRNA on Act-1 expression ... criteria of the American College of Rheumatology [18] Informed consent was provided according to the Declaration of Helsinki and obtained from all patients Approval by the ethical committee of the...

Ngày tải lên: 12/08/2014, 17:22