1 human embryonic stem cell derivation

... Health Sciences PolicyInstitute of MedicineGUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCHCommittee on Guidelines for Human Embryonic Stem Cell Research Trang 3THE NATIONAL ACADEMIES PRESS 500 Fifth ... turn away from the promisethat embryonic stem cells will provide new therapeutic advances Others believethat the derivation and application of human embryonic stem cells will underminethe dignity ... Scientific Background of Human Embryonic Stem Cell Research 29 3 Addressing Ethical and Scientific Concerns Through Oversight 47 4 Current Regulation of Human Embryonic Stem Cell Research 63 6 National

Ngày tải lên: 06/03/2014, 15:20

... Trang 1A Human Embryonic Stem Cell–based Model of Trophoblast Formation LUO WENLONG National University of Singapore 2008 Trang 2 A Human Embryonic Stem Cell–based Model of Trophoblast ... Development (poster presented at International Society for Stem Cell Research (ISSCR) Conference 2007, Cairns Australia) 2 A Human Embryonic Stem Cell–based Model of Trophoblast Formation (manuscript ... Trang 231.1 Introductory statement Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the preimplantation embryo ESCs are pluripotent stem cells as, under well established

Ngày tải lên: 11/09/2015, 16:07

... Trang 1miR-200c and GATA binding protein 4regulate human embryonic stem cell renewal Ming-Wei Sud,e, Wei Maid,e, Hsei-Wei Wangf g,h,i, , Wei-Chung Chengf k, Scott ... 2013; received in revised form 11 November 2013; accepted 20 November 2013 Abstract Human embryonic stem cells (hESCs) are functionally unique for their self-renewal ability and pluripotency, ... downregulation of miR-200c miR-200c directly targeted the 3′-untranslated Abbreviations: hESCs, human embryonic stem cells; EB, embryoid body; TGF- β, transforming growth factor-β; FGF, fibroblast growth

Ngày tải lên: 02/11/2022, 14:37

... this type of therapy, we adopted a culture system that could harvest a substantial quantity of retinal progenitor cells (RPCs) from human embryonic stem cells (hESCs) within a relatively short ... are the most promising sources of PPCs and RPCs It has been shown that embryonic stem cells (ESCs), Muller cells, mesenchymal stem cells, and some other cells can be induced to develop into RPCs ... Trang 1Experimental Study of the Biological Properties of Human Embryonic Stem Cell–Derived Retinal Progenitor Cells Jingzhi Shao1, Peng-Yi Zhou1 & Guang-Hua Peng1,2

Ngày tải lên: 24/11/2022, 17:42

... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee ... which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage ... further advanced with the development of embryonic-like stem cells, also known as induced pluripotent stem cells (iPSCs), as a potential autologous pluripotent stem cell source that can be generated

Ngày tải lên: 11/09/2015, 09:57

... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues andorgans The most common adult stem cells are the hematopoietic stem cells, mesechymalstem cells ... be performed inthe human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains of mice (Evans & Kaufman1981) Embryonic stem cell lines have been established ... immuno-privileged because of their embryonic origin.Adult stem cells versus embryonic stem cells The general differences between the characteristics of adult and embryonic stem cells aresummarised in

Ngày tải lên: 16/09/2015, 15:55

... that cancer stem cells and embryonic stem cells (ESC) have similar cell surface markers [18,19] It has been hypothesized that the similarities shared by cancer stem cells and embryonic stem cells ... cancer stem cells arise from adult stem cells present in the tissues [2] These cancer stem cells, then, have been suggested to be a subpopulation of malignant cells similar to normal stem cells, ... regulated stem cell driven differentiation and cancer stem cell reprogramming that involves altered methylation in the service of great cell heterogeneity and plasticity Keywords: Human embryonic stem

Ngày tải lên: 17/06/2020, 16:55

... Genetics, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, University of Tokyo, Japan Introduction Mouse embryonic stem (ES) cells are established from the inner ... that a homozygous mutation in the mouse Ptb gene causes embryonic lethality shortly after implantation We also established Ptb) ⁄ ) embryonic stem (ES) cell lines and found that these mutant cells ... development and ES cell proliferation Abbreviations AP, alkaline phosphatase; E, embryonic day; EB, embryoid body; ES, embryonic stem; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICM, inner cell mass;

Ngày tải lên: 07/03/2014, 00:20

... Trang 1Open AccessResearch Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice Naofumi Oyamada1, Hiroshi ... vascular endothelial growth factor receptor type 2 (VEGF-R2)-positive cells induced from mouse embryonic stem (ES) cells can differentiate into both endothelial cells (ECs) and mural cells (MCs) and ... We previously demonstrated that VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and

Ngày tải lên: 18/06/2014, 15:20

... gene transcripts in the undifferentiation of human embryonic stem cells; among the miRNAs that are highly expressed in undifferentiated embryonic stem cells, the miR-520 cluster may be closely involved ... human embryonic stem cells [17] In the present study, we applied two custom microarray platforms to detect global expression profiles of miRNAs and transcripts using three hES cell lines, embryonic ... associated with development of immune system [15] Signature miRNAs, such as the miR-302 family, the miR-200 family have been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique

Ngày tải lên: 18/06/2014, 15:20

... pro-teins in human embryonic stem cells Proc Natl Acad Sci USA 2002, 99(15):9864-9. 23. Li L, Baroja ML, Majumdar A, Chadwick K, Rouleau A, Gallacher L, et al.: Human embryonic stem cells possess ... murine embry-onic stem cells Biol Reprod 1997, 57(3):561-8. 26. Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, 24(10):2192-201. ... protects embryonic stem cells from lysis by antigen-specific CTL J Immunol 2007, 178(6):3390-9. 29 Drukker M, Katchman H, Katz G, Even-Tov Friedman S, Shezen E, Hornstein E, et al.: Human embryonic stem

Ngày tải lên: 18/06/2014, 15:20

... Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem-derived cells Trends Biotechnol ... organ Embryonic stem (ES) cells are derived from a blastocyst that potential of stem cells is their ability to develop into many different cell types, which serves as a sort of repair system for ... layers for human embryonic stem cells Biol Reprod 2003, 68, 2150-2156 4 Amit M, Shariki C, Margulets V, Itskovitz-Eldor J Feeder layer- and serum-free culture of human embryonic stem cells Biol

Ngày tải lên: 07/08/2014, 18:21

... eluci-date the exact pathways and intermediates between the embryonic stem cell and the mature adipocyte In parti-cular, mouse embryonic stem cells (mESCs) have pro-vided an invaluable tool to model ... development and the embryonic origin of this lineage remain largely unknown Results: Here we performed genome-wide analysis of gene expression during adipogenesis of mouse embryonic stem cells (ESCs) ... Trang 1R E S E A R C H Open AccessComprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development Nathalie Billon1*, Raivo

Ngày tải lên: 09/08/2014, 20:22

... AccessDefining human mesenchymal stem cell Tracey L Bonfield1*, Mary T Nolan (Koloze)1, Donald P Lennon2, Arnold I Caplan2 Abstract Allogeneic human mesenchymal stem cells (hMSCs) can suppress ... treatment with hMSCs The acute asthma mice had significantly elevated levels of systemic IL-1b (6A, n = 8, p < 0.001) Systemic IL-1b concentrations were altered by hMSCs in the acute asthma model ... after challenge (peak inflammation) hMSCs significantly decreased the level of systemic IL-1b (n = 2, p = 0.05) Systemic IFNg concentrations were increased in the acute asthma mice after hMSC treatment

Ngày tải lên: 11/08/2014, 03:20

... Prevalent in hESCs [66,67] ESC, embryonic stem cell; CTCF, CCCTC-binding factor, insulator associated protein; hESC, human embryonic stem cell; iPSC, induced pluripotent stem cell; H2AZ, histone variant ... immunoprecipitation; ChIP-seq, ChIP sequencing; DMR, differentially methylated region; ESC, embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine 4 of histone H3; H3K27ac, acetylation ... as the ‘epigenome’ [19] Abstract Human pluripotent cells such as human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) and their in vitro differentiation models hold great

Ngày tải lên: 11/08/2014, 12:21

... BMP2-expressing cells in sufficient quantities during early embryonic development in vivo Extensive investigations applying the in vitro embryonic stem (ES) cellbased developmental model in the past ... development 16 1.3E-7 GOTERM_BP_5 Nervous system development 25 5.5E-7 GOTERM_BP_5 Embryonic limb morphogenesis 12 1.8E-6 GOTERM_BP_5 Angiogenesis 17 3.7E-6 GOTERM_BP_5 Embryonic morphogenesis 14 6.7E-6 ... developed from these cells have not yet been characterized Results: We generated a transgenic BMP2 embryonic stem (ES) cell lineage expressing both puromycin acetyltransferase and enhanced green fluorescent

Ngày tải lên: 14/08/2014, 08:20

... Microtubule-associated protein MEF   Mouse embryonic fibroblast xiv  Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the ... ………………………………………………………… 1.1 Transcriptional control of stem cell pluripotency 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem cell transcriptional core 1.1.2 An expanded ... NANOG and SOX2 constitute the human embryonic stem cell transcriptional core The transcription factors OCT4, NANOG and SOX2 play essential roles in early embryonic development, and are among...

Ngày tải lên: 09/09/2015, 17:54

... of Human Embryonic Stem Cells in mTeSRTM1” (STEMCELL Technologies Inc.) In brief, H1 hESCs were maintained on BD matrigel hESC-qualified matrix (BD Biosciences) with mTeSRTM1 medium (STEMCELL ... Glial progenitor cell Homeodomain Human embryonic stem cell High mobility group Herpes simplex virus thymidine kinase Interferon Interleukin Induced pluripotent stem Luria broth Leukemia inhibitory ... deciphering the mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell...

Ngày tải lên: 10/09/2015, 09:08

... replaced by transplant-friendly and costeffective culture systems In view of the imperfections of existing culture systems, an optimal system for the culture and derivation of hESCs would require ... the culture of MEF, albeit in a separate system, for conditioned medium As stated earlier, one of the problems with large scale expansion using feeder systems that require MEFs is the limited culture ... currently no data supporting hESC proliferation on this material 1.4 Ideal hESC Culture System The various culture systems described above have sought to improve the culture of hESCs, but there are still...

Ngày tải lên: 11/09/2015, 10:06

... bp: base pair; BS-seq: whole genome bisulfite sequencing; ESC: embryonic stem cells; FDR: false discovery rate; HESC: human embryonic stem cell; HGNC: HUGO Gene Nomenclature Committee; RNA-seq: ... distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 Huang da W, Sherman BT, Lempicki RA: Systematic and integrative analysis of ... browser, Amander Clark for providing embryonic stem cell DNA (HSF1) and discussion P-YC is funded by Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA SF is Special...

Ngày tải lên: 09/08/2014, 23:20