—hiv hepatitis b virus coinfection

... of Public Health, University of Alabama at Birmingham, Birmingham, Alabama, USA. 2 Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, Alabama, ... buffered saline (PBS) Plasma albumin was determined Trang 4by using a bromocresol purple dye binding method(Sigma, St Louis, MO), and the amount of total protein was determined by using the Bradford ... system by sup-pressing immune function necessary for survival [8] * Correspondence: jollyp@uab.edu 2 Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham,...

Ngày tải lên: 20/06/2014, 08:20

... SHBs was able to bind specifically to human hepatocytes, human fibroblasts and human blood mononuclear cells but not the rat hepatocytes This binding could be inhibited by recombinant HBs but ... misfolded MHBs seems to be able to interfere with the assembly of HBV virions in the persistently producing virus cell line HepG2.215 [29, 30] Anti-preS2 antibody (that recognizes MHBs) can block HBV ... not by the recombinant LHBs The binding of SHBs with human hepatocytes was further supported by the observation from Bruin et al [39] They have found that the particles only composed by SHBs can...

Ngày tải lên: 03/11/2012, 10:09

... DiOC6,3,3¢-dihexiloxadicarbocyanine; HBeAg, hepatitis B external core antigen; HBsAg, hepatitis B surface antigen; HBV, hepatitis B virus; HBx, hepatitis B virus X protein; MMP, mitochondrial membrane potential; ... followed by substantial HBV clearance We conclude that BetA from PC could be a good candidate for anti-HBV drug development Abbreviations BetA, betulinic acid; CREB, cAMP-response element-binding ... found to have selective inhibitory effects on hepatitis B virus (HBV) In hepatocytes from HBV-transgenic mice, we showed that BetA substantially inhibited HBV replication by downregulation of manganese...

Ngày tải lên: 16/03/2014, 01:20

... (1987) The molecular biology of the hepatitis B viruses Annu Rev Biochem 56, 651–693 4 Antonucci TK & Rutter WJ (1989) Hepatitis B virus (HBV) promoters are regulated by the HBV ... November 2009, revised 22 March 2010, accepted 26 April 2010) doi:10.1111/j.1742-4658.2010.07695.x Hepatitis B virus (HBV) is a 3.2 kb DNA virus that preferentially repli-cates in the liver A number ... physiological regulation of HBV biosynthesis by bile acids in the liver will depend on both FXRa⁄ JNK-c-Jun pathway levels and the relative inhibition of SHP in the context of HBV gene expression and...

Ngày tải lên: 22/03/2014, 21:21

... increase of HBpreSAg binding to polyclonal anti-preS (black circle) before HBsAg could bring any interference based on the absorbance measurements using recombinant S protein Thus when it is below 20 ... with HBsAg and HBV DNA copies The reduction of HBpreSAg levels in conjunction with the HBV DNA copies appears to be an improved predictor of treatment outcome Background Hepatitis B virus (HBV) ... recognized Detection of HBsAg and HBpreSAg in sera from hepatitis B patients HBsAg in sera from 50 HBeAg positive and 104 HBeAg negative (anti-HBe positive) HBV patients had been quantitated (Fig...

Ngày tải lên: 18/06/2014, 18:20

... ~4000 viral segments in RefSeq has been done, building on the HBVRegDB database, and a comparative viral database (CompVirusDB) is being developed Authors' contributions NP carried out the annotations ... Web interface All virus genomes in the database can be queried by browsing a table in which information about grouping, The top part of the screenshot showing annotations of the AM282986m Hepatitis ... data-base HBVRegDB To identify conserved viral genomic regions, three blast queries (blastn, tblastx and blastx) were performed on RefSeq Virus release 24 with the parameters shown in Table 1...

Ngày tải lên: 20/06/2014, 01:20

... of HBV genotypes Keywords: Hepatitis B virus, codon usage pattern, evolution, mutation pressure, translation selection Introduction Hepatitis B virus (HBV) disease is one of the main global ... Trang 3Abstract Background: Hepatitis B virus (HBV) infection is one of the main human health problem and causes a large-scale of patients chronic infection worldwide As the replication of HBV depends ... this virus and mutation pressure from HBV itself is a main force to drive HBV evolution Genotyping of HBV is of high interest because there is increasing evidence that HBV genotypes may be associated...

Ngày tải lên: 11/08/2014, 21:22

... Inhibition of hepatitis B virus replication by shRNAs in stably HBV expressed HEPG2 2.2.15 cell lines. Arch Virol 2007, 152:871-879. 14. Ying RS, Zhu C, Fan XG, Li N, Tian XF, Liu HB, Zhang BX: Hepatitis ... MA: Inhibition of hepatitis B virus in mice by RNA interference. Nat Biotechnol 2003, 21:639-644. 16. Peng J, Zhao Y, Mai J, Pang WK, Wei X, Zhang P, Xu Y: Inhibition of hepatitis B virus replication ... polyvinylidene difluoride (PVDF) membrane (Hybond-P, Amersham Pharmacia Biotech). Blots were blocked with 5% of skim milk in TBS-T buffer for 1 h and subsequently incubated at 4°C for overnight with...

Ngày tải lên: 12/08/2014, 01:21

... Kolkata 700010, India Email: Sibnarayan Datta - sndatta1978@gmail.com Abstract Hepatitis B virus (HBV) is one of the major global public health problems In India, HBsAg prevalence among general ... epidemiology of HBV in India has been reviewed and the results of studies among the eastern Indian population have been summarised Background Hepatitis B virus (HBV) is one of the major global public health ... neighbouring Asian countries (b) Different parts of India and their HBV genotype distribution (denoted by alphabets A, C and D) are shown The prevalent HBV genotype is denoted by bold alphabet...

Ngày tải lên: 12/08/2014, 04:21

... HBV, mà chỉ biểu hiện đã từng mắc HBV Bảng 1 Các dấu ấn chỉ thị sự nhiễm HBV Quá trình nhiễm Dấu ấn Cấp tính Mãn tính Nhiễm trong quá khứ HBsAg + + - HBeAg ban đầu +, sau đó - +/- - anti-HBc ... hiện ngay bước biến nạp nhằm đưa sản phẩm nối vào chủng biểu hiện E coli BL21(DE3) 3.7.8 Biến nạp vector tái tổ hợp vào tế bào chủ E coli a Chuẩn bị tế bào khả nạp - Cấy 50µl E coli BL21(DE3) ... PreS2 của virus viêm gan siêu vi B - Hepatitis B virus (HBV) trong E coli; Lê Thái Hoàng, 2004 * Tinh chế và khảo sát tính miễn dịch nguyên của protein tái tổ hợp PreS1S226 của virus gây bệnh viêm...

Ngày tải lên: 09/02/2015, 04:05

... causes of human hepatitis, few are of greater global importance than hepatitis B virus (HBV) HBV infection is a major public health concern globally for several important reasons First, HBV infection ... function tests (LFTs) Chronic hepatitis B (CHB) HBsAg +ve with abnormal LFTs and increased HBV DNA Compensated cirrhosis (CC) Cirrhosis based on liver biopsy or obvious findings on ultrasound ... a pregnant woman is an HBV carrier and is also HBeAg-positive, her newborn baby has 90% likelihood to be infected and become a carrier Of these children, 25% will die subsequently from chronic...

Ngày tải lên: 14/09/2015, 09:38

... OF ABBREVIATIONS AFP Alpha-fetoprotein AMV Avian Myeloblastosis Virus anti-HBcAg Antibody to hepatitis B core antigen anti-HBeAg Antibody to hepatitis B e antigen anti-HBsAg Antibody to hepatitis ... to hepatitis B core antigen (anti-HBcAg) Another clinical useful antigen-antibody system that has been identified for hepatitis B is hepatitis B e antigen (HBeAg)/antibody to HBeAg (anti-HBe) ... 321.2.2 Hepatitis B: the Disease Viral hepatitis is an inflammation of the liver caused by hepatitis viruses Five different hepatitis viruses have been identified The term “hepatitis B” was first...

Ngày tải lên: 14/09/2015, 12:11

... valine Others anti-HBc antibody against hepatitis B core antigen anti-HBe antibody against hepatitis B e antigen anti-HBs antibody against hepatitis B surface antigen COUP-TF1 ovalbumin upstream promoter-transcription ... genotyped 76 Table 8 Comparison of Characteristics in chronic hepatitis B patients with Table 9 Clinical data of 52 chronic hepatitis B patients in HBV DNA Table 10 HBV DNA levels in genotype B and C ... of HBsAg 28 Table 3 Geographical distribution of HBV genotypes and subtypes 29 Table 4 Different mutations in the precore/core gene 46 Table 7 Characteristics of the 71 chronic hepatitis B patients...

Ngày tải lên: 15/09/2015, 21:28

... Human hepatitis B virus belongs to the hepadnavirus family, which is made up of the genus orthohepadnavirus with members found in mammals: HBV, woodchuck hepatitis virus (WHV), ground squirrel hepatitis ... ground squirrel hepatitis virus (GSHV); and the genus avihepadnavirus which consists of duck hepatitis B virus (DHBV) and grey heron hepatitis B virus (HHBV) The name hepadnavirus reflects the hepatotropism ... period of incubation, and hepatitis B for the form transmitted through blood products and displaying a longer incubation period (5) The hepatitis B virus was the first human hepatitis virus from...

Ngày tải lên: 16/09/2015, 08:30

... circle replication Mb per run 100 Mb 600,000 Mb 170,000 Mb 100 Mb 180,000 Mb Time per run 7 hours 9 days 9 days 1.5 hours 12 days Read length 400 bp 2x100 bp 35x75 bp 200 bp 35 bp (mate-pair) Cost ... FDR False Discovery Rate HBV Hepatitis B Virus HBx Hepatitis B virus X gene HCC Hepatocellular Carcinoma HOMER Hypergeometric Optimization of Motif Enrichment MACS Model-based Analysis for ChIP-Seq ... Platform noises die bias & hybridization noise possible GC bias Multiplexing no yes by using library index or barcode Peak detection fewer peaks with broader width larger number of more localized...

Ngày tải lên: 03/10/2015, 21:56

... Y khoa Hòa Hảo cung cấp (bảng 23) Bảng 23 Kết quả định lượng HBVvới kit Bayer Versant HBV và kit định lượng HBV Bệnh phẩm Kit Versant (bản sao/ml) Kit HBV định lượng (bản sao/ml) Hình 11 Hệ ... lượng bản sao bộ gene của tác nhân gây bệnh trong bệnh phẩm đạt 104 bản sao/ml Các phương pháp sử dụng mẫu dò có kết hợp khuếch đại tín hiệu như bDNA (branched DNA probe) (Chiron) hay QB replicase ... kit HBV định tính và kit Versant (Bayer) Bệnh phẩm Kit Versant (bản sao/ml) Kit HBV định tính Kết quả (bảng 3) cho thấy : - 19 mẫu dương tính với kit HBV định tính có hàm lượng HBV từ 3.261 bản...

Ngày tải lên: 04/02/2019, 23:04

... strategy Keywords: Hepatitis B immunoglobulin, Hepatitis B virus, Meta-analysis, Mother-to-child transmission Background Hepatitis B virus (HBV) infections are a global health problem [1] Studies ... (schedule/infant month) HBsAg+ HBsAb+ HBsAg+ HBsAb+ HBsAg+ HBsAb+ Ji 2003 [ 30 ] 21-31 1 T: HBIG 200 IU (7,8,9) NR T:29 T:3 T:10 NR NR NR NR Xu 2006 [ 31 ] NR 1 T: HBIG 200 IU (7,8,9) NR T:30 ... (schedule/month ) Sample size (n) Newborn 7-12month infant >12 month child HBsAg-pos HBsAb-pos HBsAg-pos HBsAb-pos HBsAg-pos HBsAb-pos Lo 1985 [ 59 - 61 ] 1 T: HBIG50 IU(0) + PDV5ug T:36 NR NR...

Ngày tải lên: 27/02/2020, 13:46

... scan of abdominal abdomen with contrast or CHT with contrast inhibitors + AFP higher than normal (less than 400ng / ml) + hepatitis B, C virus infection - Patients with HCC have positive HBsAg test ... chronic hepatitis B: We selected patients with chronic hepatitis B diagnosed under the guidance of the Ministry of Health of Vietnam in 2014 - HBsAg (+) > 6 months or HBsAg (+) and Anti HBc IgG ... miRNA in HCC patients infected with hepatitis B virus Therefore, we conducted the study: “Research on expression of microRNA-21, microRNA-122 plasma in hepatitis B virus-related hepatocellular carcinoma...

Ngày tải lên: 29/09/2020, 15:24

... Pal1, Shivram Prasad Singh3, Avik Biswas1, Shekhar Chakrabarti1,4and Runu Chakravarty1* Abstract Background: Hepatitis B Virus (HBV) X protein (HBx) is known to be involved in the initiation and ... electrophor-esis Membranes were blocked in TBS-0.1% Tween 20 (TBS-T)/5% (w/v) milk for 1 hour at room temperature Membranes were then incubated with primary anti-bodies diluted in TBS-T for 4 hour ... temperature Subsequently, membranes were washed with TBS-T and incubated with peroxidase-conjugated secondary anti-body diluted in TBS-T at room temperature for 30 mi-nutes Membranes were washed in TBS-T...

Ngày tải lên: 14/10/2020, 16:14

... n (%) Abbreviations: HBV hepatitis B virus, SD standard deviation, US United States. a HBV screening means that both hepatitis B surface antigen (HBsAg) test and antibody to hepatitis B core ... National Comprehensive Cancer Network, HBsAg Hepatitis B surface antigen, anti-HBc Antibody to hepatitis B core antibody, anti-HBs Antibody to hepatitis B surface antigen. Trang 33 Patient Accounts: ... antibody to hepatitis B core antibody (anti-HBc), alanine aminotransferase, total bilirubin, and HBV DNA HBV screening and infection Screening was defined as having both HBsAg and anti-HBc tests...

Ngày tải lên: 05/11/2020, 05:33