—cigarette smoke gas phase components

... highly controlled and accurately reproducible cell exposure to gas phase CS that closely resembles the dosage and gas kinetics of CS in the smokers' lung, in conjunction with standard techniques to ... profile, minute puff cycle) In order to remove the particulate matter and obtain gas phase smoke (GPS), the cigarette smoke was passed through Cambridge filters rated to withhold 99.9% of all particles ... Lagesson V, Stavridou AJ, Pouli AE, LagessonAndrasko L, Stavrides JC: Analysis of the gas phase of cigarette smoke by gas chromatography coupled with UV-diode array detection Anal Chem 2006, 78:4509-16...

Ngày tải lên: 12/08/2014, 14:20

... and M phases in a typical cell cycle DNA replication (S -phase) and cell division (mitosis, M phase) are separated by distinct gap phases Physical and temporal separation of DNA synthesis (S -phase) ... and mitosis (M phase) implies existence of gap phases separating these events (Fig 1) Gap phase (G1) is defined as the period from end of mitosis to initiation of DNA synthesis Gap phase (G2) separates ... periods or phases, an organizing principle distinguishing bacteria from eukaryotic cells Molecular mechanisms are therefore required to coordinate these processes in time and space G1 Phase: Components, ...

Ngày tải lên: 25/10/2013, 21:20

... Ciliastatic components in the gas phase of cigarette smoke Science 1966, 153:1248-1250 182 Wynder EL, Goodman DA, Hoffmann D: Ciliatoxic components in cigarette smoke In vitro comparison of different smoke ... pickup rate was more sensitive to the gas than the particulate phase of mainstream and sidestream smoke solutions [129] Since pick-up rate decreased in sidestream smoke when beat frequency increased, ... Sidestream smoke generally produced a stronger effect in all assays than mainstream smoke solution Smoke from the 1R4F cigarettes, which more closely resemble the commercial brands smoked today...

Ngày tải lên: 05/03/2014, 17:20

... effects of these peptides on the cigarette smoke- induced toxicity in the lung have not been elucidated In the present study, we found that exposure to cigarette smoke extract (CSE) induced significant ... The toxicity of cigarette smoke is closely associated with the occurrence of COPD in developing countries, and a number of in vitro studies have shown that cigarette smoke induces the apoptosis ... may be involved in the toxicity Recently, it has been reported that numerous chemical components of cigarette smoke, including acrolein [44], nicotine [45], Benzo[a]pyrene [46], and N-nitrosamines...

Ngày tải lên: 07/03/2014, 15:20

Ngày tải lên: 09/03/2014, 10:20

Ngày tải lên: 24/03/2014, 01:20

... are credited In summary, the presence of voids during the growth of thin SiC layers by solid gas phase epitaxy has been confirmed experimentally by scanning electron microscopy and micro-Raman ... 5:1507–1511 b from a void out of void 250 200 Si 2nd order (~970) TO SiC (~794) exp data fitting fitting components Raman Intensity, a u a Raman Intensity, a u Fig a Raman spectra from SiC layer grown ... SiC films on Si(111) was achieved using the chemical reaction of monocrystalline silicon and CO gas, supplied at a rate of 1–10 ncm3/min and a pressure of 0.1–10 Torr Growth occurs in the temperature...

Ngày tải lên: 21/06/2014, 17:20

... Lung HDAC2 activity and KC expression in smoke- exposed mice wt PI3Kγ-/- PI3KδD910/A910 Sham Smoke Smoke + Budesonide Sham Smoke Smoke + Budesonide Sham Smoke Smoke + Budesonide Lung HDAC-2 activity ... exposed sham Abbreviations; Smoke: Smoke Exposed; Bud: Budesonide Figure Cigarette smoke exposure reduces Mi-2α expression but elevates Mi-2β expression in the lungs Cigarette smoke exposure reduced ... Abbreviations; Smoke: Smoke Exposed; Bud: Budesonide protected against the down regulation of Mi2α protein in the lungs of both PI3Kγ-/- and PI3KD910/A910 smokeexposed mice compared to smoke exposed...

Ngày tải lên: 11/08/2014, 03:20

... thickening in apoE-/- mice exposed to cigarette smoke Atherosclerosis 2004, 175:7-14 Gairola CG, Drawdy ML, Block AE, Daugherty A: Sidestream cigarette smoke accelerates atherogenesis in apolipoprotein ... MicroDust Pro-aerosol monitor (Casella CEL, Bedford, UK) and verified daily by gravimetric sampling The smoke concentration was set at a nominal value of approximately 300 mg/m TPM by adjusting the flow ... evidenced earlier [12,13] It has been shown that eNOS regulates endothelial function and several components of the atherogenic process, such as vascular smooth muscle cell contraction, proliferation,...

Ngày tải lên: 11/08/2014, 03:20

... Lung HDAC2 activity and KC expression in smoke- exposed mice wt PI3Kγ-/- PI3KδD910/A910 Sham Smoke Smoke + Budesonide Sham Smoke Smoke + Budesonide Sham Smoke Smoke + Budesonide Lung HDAC-2 activity ... exposed sham Abbreviations; Smoke: Smoke Exposed; Bud: Budesonide Figure Cigarette smoke exposure reduces Mi-2α expression but elevates Mi-2β expression in the lungs Cigarette smoke exposure reduced ... Abbreviations; Smoke: Smoke Exposed; Bud: Budesonide protected against the down regulation of Mi2α protein in the lungs of both PI3Kγ-/- and PI3KD910/A910 smokeexposed mice compared to smoke exposed...

Ngày tải lên: 11/08/2014, 06:22

... thickening in apoE-/- mice exposed to cigarette smoke Atherosclerosis 2004, 175:7-14 Gairola CG, Drawdy ML, Block AE, Daugherty A: Sidestream cigarette smoke accelerates atherogenesis in apolipoprotein ... MicroDust Pro-aerosol monitor (Casella CEL, Bedford, UK) and verified daily by gravimetric sampling The smoke concentration was set at a nominal value of approximately 300 mg/m TPM by adjusting the flow ... evidenced earlier [12,13] It has been shown that eNOS regulates endothelial function and several components of the atherogenic process, such as vascular smooth muscle cell contraction, proliferation,...

Ngày tải lên: 11/08/2014, 06:22

... cigarette smoke exposure from cigarettes/animal/day in a smoke chamber An Indian commercial filter-tipped cigarette (74 mm) with a tar content of 15 mg and nicotine content of mg was used The smoke ... vacuum was turned off and the guinea pig was further exposed to the smoke for another 30 sec The total duration of exposure to smoke form one puff was thus 40 sec The amount of suspended particle ... are given BT infusion along with smoke exposure The enlargement of air space is appar- A, Oxyblot of lung proteins of guinea pigs exposed to air or cigarette smoke (CS) with or without giving...

Ngày tải lên: 11/08/2014, 08:21

... days of vitamin C deprivation, the guinea pigs were subjected to cigarette smoke exposure from cigarettes/animal/day in a smoke chamber, along with supplementation of vitamin C (1 mg, mg or 15 mg/animal/day), ... turned off and the guinea pig was further exposed to the accumulated smoke for another 40 sec The total duration of exposure to smoke from one puff was thus 45 sec The amount of suspended particle ... independent sections in each group were evaluated Preparation of aqueous extract of cigarette smoke Smoke from an Indian commercial filter-tipped cigarette (74 mm, tar content 20 mg, nicotine content...

Ngày tải lên: 11/08/2014, 08:22

... CSM: Cigarette Smoke Medium; CS: Cigarette smoke; IL-8: interleukin-8; NAC: N-acetyl-L-cysteine; OD: Optical Density; TNF-α: Tumor necrosis factor-α; GSH: Glutathione; CS: Cigarette smoke; MDMs: ... described below Cigarette smoke medium preparation CSM was prepared as described before [9] Briefly, a smoking machine (Teague Enterprises, Davis, CA, USA) was programmed to smoke cigarette according ... utilizing signals from the Toll-like receptors (TLRs) TLRs recognize specific patterns of microbial components [10] and signals to initiate a range of host defense mechanisms [11] TLR4 is a crucial...

Ngày tải lên: 11/08/2014, 08:22

... expression in both endothelial cells and mouse lung [28], and in emphysematous lungs of both smokers and non-smokers [4,29] However, the mechanism of CS-induced VEGFR2-mediated impaired Akt and its ... endothelial dysfunction Cigarette smoke exposure had no effect on activation of caspase or lung cell death Increased endothelial cell death was observed in emphysematous lungs of smokers indicate that apoptotic ... of Figure Effect of CS smoke on active caspase activation in rat lungs (A) Representative pictures show the IHC staining of active caspase at days, weeks and months CS smoke- exposed rat lungs...

Ngày tải lên: 11/08/2014, 08:22

... attenuated cigarette smoke- induced pulmonary artery remodeling and pulmonary hypertension As compared with hamsters exposed to cigarette smoke alone, the hamsters exposed to cigarette smoke plus mg/kg ... intraperitoneal injection to hamsters 0.5 h before the first smoke exposure each day Hemodynamic analysis At the end of four months of smoke exposure, right ventricular systolic pressure (RVSP) ... P < 0.05 Results Chronic cigarette smoke exposure leads to pulmonary artery remodeling and pulmonary hypertension in hamsters After four months of cigarette smoke exposure, thickwalled pulmonary...

Ngày tải lên: 12/08/2014, 11:21

... difference in the numbers of Langerhans cells in the airway biopsies of smokers with COPD when compared to ex-smokers or non-smokers without COPD [6] The current study was designed to determine ... smokers with COPD, and suggests the possibility that cigarette smoke- induced osteopontin levels in the lung may be involved in recruitment or formation of Langerhans type dendritic cells in smokers ... determine mechanisms by which cigarette smoke constituents promote dendritic cell persistence in the lung Specifically, we sought to determine whether cigarette smoke promotes dendritic cell retention...

Ngày tải lên: 12/08/2014, 11:21

... Cigarette smoke is conventionally described as having two phases: the tar phase (the > μm particulate material trapped when smoke is passed through a standard Cambridge glass-fiber filter) and the gas ... sidestream cigarette smoke: nature of the free radicals in gas- phase smoke and in cigarette tar Environ Health Perspect 1983, 47:345-355 32 Pryor WA, Stone K: Oxidants in cigarette smoke Radicals, ... unfiltered cigarette smoke with media, solubilization of smoke material collected on a filter, direct cell exposure to cigarette smoke, as well as testing of individual components [37-41] Each...

Ngày tải lên: 12/08/2014, 11:22

... cigarette smoke exposure Although IL-10 levels were rising after smoking cessation, the smoke- induced reduction was Table Immune cells in BALF recovered from air-exposed mice, smoke- exposed mice and smoke- exposed ... Smoke Smoke cessation Figure Cigarette smoke- induced alveolar enlargement is irreversible Representative photomicrographs of hematoxylin and eosin stained lung tissue of air-exposed mice (A), smoke- exposed ... inflammatory cell influx in bronchoalveolar lavage fluid 0.30 0.20 0.15 0.10 Control Smoke Smoke cessation Figure Cigarette smoke- induced right ventricle heart hypertrophy is irreversible Right ventricle...

Ngày tải lên: 12/08/2014, 11:22

... http://respiratory-research.com/content/11/1/100 Page of Table Bronchoalveolar analysis 2-wk Air 2-wk Smoke 4-wk Air 4-wk Smoke 24-wk Air 24-wk Smoke Total cell number, (× 103) 602.5 ± 41.20 797.53 ± 74.96* 410.00 ± 144.12 ... 0.0 Air number of CD3+ cells/field c B Smoke 4-wk nose 2.0 1.5 1.0 0.5 0.0 Air C Smoke 24-wk nose number of CD3+ cells/field 5.0 4.0 3.0 2.0 1.0 0.0 Air Smoke Figure CD3+ staining Nasal turbinate ... using TaqMan probes Methods Mouse model of Cigarette Smoke exposure Groups of Male C57BL/6 mice, 6-8-week old were exposed to the tobacco smoke of five cigarettes (Reference Cigarette 2R4F without...

Ngày tải lên: 12/08/2014, 11:22