Nghiên cứu phân lập hợp chất β sitosterol glucoside từ phân đoạn dịch chiết chloroform và xây dựng tiêu chuẩn cơ sở cao chiết nước của hoa đu đủ đực thu hái tại quảng nạm đà nẵng

Cao chiӃt tәQJQj\ÿѭӧc chӃ WKrPQѭӟc và chiӃt phân lӟp lҫQOѭӧt vӟi n-hexan, chloroform, ethyl acetate và butanol bҵng phӉu chiӃt.. Vӟi mӛi loҥi dung môi thӵc hiӋn chiӃt 3 lҫn.. Các dӏch ch

1*8<ӈ1 7+ӎ.,0È1+

1*+,Ç1&Ӭ83+Æ1/Ұ3 +Ӧ3&+Ҩ7 ȕ-SITOSTEROL GLUCOSIDE 7Ӯ

3+Æ1Ĉ2Ҥ1'ӎ&+&+,ӂ7 CHLOROFORM 9¬;Æ<'Ӵ1*7,Ç8&+8Ҭ1

&Ѫ6Ӣ&$2&+,ӂ71ѬӞ& &Ӫ$+2$Ĉ8ĈӪĈӴ& 7+8+È,7Ҥ,48Ҧ1*

NAM-Ĉ¬1Ҹ1*

/8Ұ19Ă1 &Ӱ1+Æ1+Ï$+Ӑ&

Ĉj1 ҹng- 1ăP 2019

1*8<ӈ1 7+ӎ.,0È1+

1*+,Ç1&Ӭ83+Æ1/Ұ3 +Ӧ3&+Ҩ7 ȕ-SITOSTEROL GLUCOSIDE 7Ӯ

3+Æ1Ĉ2Ҥ1'ӎ&+&+,ӂ7 CHLOROFORM 9¬;Æ<'Ӵ1*7,Ç8&+8Ҭ1

&Ѫ6Ӣ&$2&+,ӂ71ѬӞ& &Ӫ$+2$Ĉ8ĈӪĈӴ& 7+8+È,7Ҥ,48Ҧ1*

NAM-Ĉ¬1Ҹ1*

/8Ұ19Ă1 &Ӱ1+Æ1+Ï$+Ӑ&

NJѭӡLKѭӟQJGүQNKRDKӑF : ThS Ĉӛ7Kӏ7K~\9kQ

Ĉj1 ҹng-1ăP 2019

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4 3KѭѫQJSKiSQJKLrQFӭX 2

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2.3.1 Alkaloid 14

2.3.2 Flavonoid 15

2.3.3 Coumarin 15

2.3.4 Saponin 15

Ĉ˱ͥQJNK͵ 16

2.3.6 Polyphenol 16

2.3.7 Steroid 16

2.3.8 Acid KͷXF ˯ 16

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2.3.10 Carotene 16

2.3.11 Polysaccairid 17

2.3.12 Iridoid 17

6Ѫ ĈӖ Ĉ,ӄU CHӂ CÁC CAO CHIӂT 17

2.5 THӰ HOҤT TÍNH SINH HӐC CӪA DӎCH CHIӂT CHLOROFORM 18

2.6 CHҤY CӜT SҲC KÝ PHҪN CAO CHLOROFORM 19

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3+Ө/Ө&

DMSO : Dimethyl sunfoxide

DEPT : Distortionless enhancement by polarisation transfer

EtOAc : Ethyl acetate

EtOH : Ethanol

HMBC : Heteronuclear Multiple Bond Correlation

HSQC : Heteronuclear Single Quantum Corelation

2.3 6ѫÿӗ phân lұp hӧp chҩt tӯ phân ÿRҥn dӏch chiӃt chloroform

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Trong dân gian lá cây ÿu ÿӫÿѭӧFVӱGөQJÿӇViWNKXҭQNKiQJQҩPNKiQJYLrP

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WtQKFKӕQJR[\KyDQj\GRFiFKӧSFKҩWSKHQROJk\UD/iÿu ÿӫFyKRҥWWính kháng

NKXҭQWӕWFyNKҧQăQJNKiQJQKLӅXORҥLYLNKXҭQJUDPkPJUDPGѭѫQJFiFORҥLQҩP

ӢQѭӟFWDFDRFKLӃWYӟLFӗQWӯOiÿu ÿӫÿѭӧFQJKLrQFӭXWURQJPӝWVӕP{KuQK

³1JKLrQ FͱX SKkQ O̵S KͫS FK̭W ȕ-sitosterol glucoside Wͳ SKkQ ÿR̩Q G͓FK FKL͇W

chloroform Yj[k\G͹QJWLrXFKX̱QF˯VͧFDRFKL͇WQ˱ͣF  FͯDKRD ĈX  ÿͯÿ͹FWKXKiLW̩L

&KѭѫQJ± ӃWTXҧYjWKҧROXұQ (11 trang)

.ӃWOXұQ YjNLӃQQJKӏ (1 trang)

7jLOLӋXWKDPNKҧR (5 trang)

3KөOөF trang)

7Ә1*48$17¬,/,ӊ8

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ĈX ÿӫ Carica Papaya /  WKXӝF Kӑÿu ÿӫ &DULFDFHDH  QJXӗQ JӕF &KkX 0ӻ

ÿѭӧFWUӗQJNKҳSQѫLӣQѭӟFWD +ӑÿu ÿӫ&DULFDFHDH WUrQWKӃJLӟLJӗm có 4 chi và 45

loài [16] Cây ÿu ÿӫFyWrQNKRDKӑFOjCarica papaya /LQQ&k\QKӓKRһFQKӥFDRWӯ

2 - PpWWKkQWKҷQJNK{QJSKkQQKiQK/iWRPӑFVROHWұSWUXQJӣQJӑQ&XӕQJOi

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KҷQKRjQWRjQÿӵFFiLKD\OѭӥQJWtQKPjOҥLSKDOүQQKLӅXtWÿһFWtQKFӫDEDORҥLKRD

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FKXQJ FӫD QKyP Fk\ Qj\ Oj VLQK WUѭӣQJ NKӓH Oi [DQK ÿұP VRQJ SKLӃQ Oi PӓQJ

alkaloid carpaine trong lá ÿu ÿӫ>@

1ăP  +j 7Kӏ %tFK 1JӑF ÿm Vӱ GөQJ Nӻ WKXұW +3/& SKkQ WtFK FiF FKҩW

carotenoid trong lá ÿu ÿӫ.ӃWTXҧFKRWKҩ\ȕ-FDURWHQHOXWHLQHFKLӃPWӹOӋWѭѫQJӭQJ

OjYjVRYӟLWәQJFiFFKҩWFDURWHQRLGWX\QKLrQNK{QJ[iFÿӏQKÿѭӧFlycopene [9]

FӫDOiÿu ÿӫ%DRJӗPȕ-sitosterol, daucosterol, cycloart -23-ene-ȕ-diol (sterculin

A) và cycloart-25-ene-ȕ 56 -GLRO 7URQJ ÿy VWHUFXOLQ  $ Yj F\FORDUW

EҵQJFiFGXQJP{LFyÿӝSKkQFӵFWăQJGҫQQ-hexan, CH2Cl2(W2$FEXWKDQRO 7ӯ

FһQ FKLӃW &+2Cl2 SKkQ OұS ÿѭӧF  KӧS FKҩW GDQLHORQH FDUSDLQRQHacid pluchoiF

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1ăP*LDQJ7Kӏ.LP/LrQYjĈӛ7Kӏ/Ӌ8\rQNKҧRViWWKjQKSKҫQKyDKӑF

sterol trong hoa ÿu ÿӫÿӵFWKXKiLWҥLĈj1ҹQJ>@

1*+,Ç1&Ӭ89ӄ7+¬1+3+Ҫ1+Ï$+Ӑ&&Ӫ$&Æ< Ĉ8 ĈӪ NGOÀI

1ѬӞ&

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ÿӫ>]

protocatechuic; kaempferol; quercetin và 5,7- GLPHWKR[\FRXPDLU&ҩXWU~FSKkQWӱFӫD

OH OH OH

(11) 5,7-dimethoxycoumair (12) Acid protocatechuic (13) Acid chlorogenic

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x 4XҧSURWHLQFKҩWEpR[HQOXOR]DFDUERK\GUDWHFKҩWNKRiQJ&D3)HYLWDPLQ

& % % QLDFLQ Yj FDURWHQH DPLQRacid DFLW FLWULF DFLG PDOLFTXҧ [DQK  OLQDORRO

benzylisothiocyanate, cisYjtrans 2,6-dimethyl -3,6 epoxy-7 octen-2-ol, alkaloid,

carpain, benzy±ȕ-D-glucoside, 2-phenylethyl±ȕ-D-glucoside, 4-hydro[\SKHQ\O-HWK\O

±ȕ-'-JOXFRVLGHYjÿӗQJSKkQEHQ]\O-ȕ-D-glucoside

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palmatic, stearic, lioleic, linolenic, cis-YDFFHQLFYjROHLF

x +ҥW DFLG IDWW\ SURWHLQ FKҩW [ѫ GҫX FDUSDLQH EHQ]\OLVRWKLRF\DQDWH

1ăPStephen &KLQZHQGXYjFӝQJVӵ F{QJEӕ³1JKLrQFӭXWKjQKSKҫQKyD

KӑFFӫDKRDÿu ÿӫӣ1LJHULD´&KRNӃWTXҧWURQJKRDFKӭDVDSRQLQ (0.07%), alkaloid

(0.05%), tannin (0.002%) và flavonoid  ... loҥLGXQJP{LWKXÿѭӧc 70g cao n-hexan

Phân lӟp vӟi chloroform, chiӃt lҫn, vӟi khoҧng 15 lít chloroform Tәng dӏch

chiӃt khoҧng 14000mL, cҩt loҥLGXQJP{LWKXÿѭӧc 52g cao chloroform

Phân lӟp... Ӆ u chӃ cao n-hexan, chloroform, EtOAc cao BuOH

Dӗn dӏFKÿһc sau lҫn chiӃt cҩt loҥi bӟt dung môi, phân lӟp lҫQOѭӧt vӟi

n-hexan, CHCl3, EtOAc BuOH

Phân lӟp... khơng, thu

ÿѭӧc cao chiӃt tәng Có thӇ JLD WăQJ KLӋu quҧ chiӃt bҵng cách thӍnh thoҧQJÿҧo lӝn,

xӕFÿӅXKRăFVӱ dөng máy siêu âm Cao chiӃt tәQJQj\ÿѭӧc chӃ WKrPQѭӟc chiӃt

phân