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Preview Biology,12th Edition by Peter H. Raven, George B. Johnson, Kenneth A. Mason, Jonathan Losos, Tod Duncan (2019) Preview Biology,12th Edition by Peter H. Raven, George B. Johnson, Kenneth A. Mason, Jonathan Losos, Tod Duncan (2019) Preview Biology,12th Edition by Peter H. Raven, George B. Johnson, Kenneth A. Mason, Jonathan Losos, Tod Duncan (2019) Preview Biology,12th Edition by Peter H. Raven, George B. Johnson, Kenneth A. Mason, Jonathan Losos, Tod Duncan (2019)

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Peter H Raven

President Emeritus, Missouri Botanical Garden;

George Engelmann Professor of Botany Emeritus, Washington University

George B Johnson

Professor Emeritus of Biology, Washington University

Twelfth Edition

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BIOLOGY, TWELFTH EDITION

Published by McGraw-Hill Education, 2 Penn Plaza, New York, NY 10121 Copyright © 2020 by McGraw-Hill Education All rights

reserved Printed in the United States of America Previous editions © 2017, 2014, and 2011 No part of this publication may be reproduced

or distributed in any form or by any means, or stored in a database or retrieval system, without the prior written consent of McGraw-Hill

Education, including, but not limited to, in any network or other electronic storage or transmission, or broadcast for distance learning.

Some ancillaries, including electronic and print components, may not be available to customers outside the United States.

This book is printed on acid-free paper

1 2 3 4 5 6 7 8 9 LWI 21 20 19

ISBN 978-1-260-16961-4 (bound edition)

MHID 1-260-16961-8 (bound edition)

ISBN 978-1-260-49470-9 (loose-leaf edition)

MHID 1-260-49470-5 (loose-leaf edition)

Portfolio Managers: Andrew Urban, Michelle Vogler

Product Developers: Elizabeth Sievers, Joan Weber

Marketing Manager: Kelly Brown

Content Project Managers: Kelly Hart, Brent dela Cruz, Sandy Schnee

Buyer: Susan K Culbertson

Design: David W Hash

Content Licensing Specialists: Lori Hancock

Cover Image: (Diatom) ©Steve Gschmeissner/Science Photo Library/Getty Images; (Leaf): ©Lee Chee Keong/EyeEm/Getty Images;

(Rhinoceros): ©GlobalP/iStock/Getty Images Plus; (Beetle): ©kuritafsheen/ooM/Getty Images; (Chameleon): ©SensorSpot/E+/Getty

Images; (DNA): ©Doug Struthers/The Image Bank/Getty Images; (Jellyfish): ©Raghu_Ramaswamy/iStock/Getty Images Plus

Compositor: MPS Limited

All credits appearing on page or at the end of the book are considered to be an extension of the copyright page.

Library of Congress Cataloging-in-Publication Data

Mason, Kenneth A., author | Losos, Jonathan B., author | Duncan, Tod, author

Biology / Kenneth A Mason, University of Iowa, Jonathan B Losos,

Washington University, Tod Duncan, University of Colorado, Denver;

contributors, Charles J Welsh, Duquesne University

Twelfth edition | New York, NY : McGraw-Hill Education, [2020]

| “Based on the work of Peter H Raven, President Emeritus, Missouri

Botanical Garden; George Engelmann, Professor of Botany Emeritus,

Washington University, George B Johnson, Professor Emeritus of Biology,

Washington University.” | Includes index

LCCN 2018036968| ISBN 9781260169614 (alk paper) |

ISBN 9781260565959

LCSH: Biology—Textbooks

LCC QH308.2 R38 2020 | DDC 570—dc23

LC record available at https://lccn.loc.gov/2018036968

The Internet addresses listed in the text were accurate at the time of publication The inclusion of a website does not indicate an

endorsement by the authors or McGraw-Hill Education, and McGraw-Hill Education does not guarantee the accuracy of the information

presented at these sites.

mheducation.com/highered

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Brief Contents

Committed to Excellence xi

Preparing Students for the Future xv

1 The Science of Biology 1

2 The Nature of Molecules and the Properties of Water 18

3 The Chemical Building Blocks of Life 35

4 Cell Structure 63

5 Membranes 92

6 Energy and Metabolism 112

7 How Cells Harvest Energy 128

8 Photosynthesis 154

9 Cell Communication 176

10 How Cells Divide 194

11 Sexual Reproduction and Meiosis 217

12 Patterns of Inheritance 231

13 Chromosomes, Mapping, and the Meiosis–Inheritance

Connection 250

14 DNA: The Genetic Material 268

15 Genes and How They Work 290

16 Control of Gene Expression 317

17 Biotechnology 340

18 Genomics 366

19 Cellular Mechanisms of Development 389

20 Genes Within Populations 416

21 The Evidence for Evolution 443

22 The Origin of Species 463

23 Systematics, Phylogenies, and Comparative Biology 484

24 Genome Evolution 504

25 The Origin and Diversity of Life 523

37 Plant Nutrition and Soils 807

38 Plant Defense Responses 825

39 Sensory Systems in Plants 838

40 Plant Reproduction 866

41 The Animal Body and Principles of Regulation 900

42 The Nervous System 924

43 Sensory Systems 955

44 The Endocrine System 982

45 The Musculoskeletal System 1006

46 The Digestive System 1026

47 The Respiratory System 1047

48 The Circulatory System 1066

49 Osmotic Regulation and the Urinary System 1088

50 The Immune System 1106

51 The Reproductive System 1135

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Kenneth Mason maintains an association with the University of Iowa, Department of Biology after having served

as a faculty member for eight years His academic positions, as a teacher and researcher, include the faculty of the University of Kansas, where he designed and established the genetics lab, and taught and published on the genetics of pigmentation in amphibians At Purdue University, he successfully developed and grew large intro- ductory biology courses and collaborated with other faculty in an innovative biology, chemistry, and physics course supported by the National Science Foundation At the University of Iowa, where his wife served as president of the university, he taught introductory biology and human genetics His honor society memberships include Phi Sigma, Alpha Lambda Delta, and, by vote of Purdue pharmacy students, Phi Eta Sigma Freshman Honors Society.

Jonathan Losos is the William H Danforth Distinguished University Professor in the Department of Biology

at Washington University and Director of the Living Earth Collaborative, a partnership between the university, the Saint Louis Zoo and the Missouri Botanical Garden Losos’s research has focused on studying patterns

of adaptive radiation and evolutionary diversification in lizards He is a member of the National Academy

of Sciences, a fellow of the American Academy of Arts and Science, and the recipient of several awards, including the Theodosius Dobzhanksy and David Starr Jordan Prizes, the Edward Osborne Wilson Naturalist Award, and the Daniel Giraud Elliot Medal, as well as receiving fellowships from the John Guggenheim and David and Lucile Packard Foundations Losos has published more than 200 scientific articles and has written two books, Lizards in an Evolutionary Tree: Ecology and Adaptive Radiation of Anoles (University of California Press, 2009) and Improbable Destinies: Fate, Chance, and the Future of Evolution (Penguin-Random

House, 2017).

Tod Duncan is a Clinical Assistant Professor at the University of Colorado Denver He currently teaches first semester general biology and coordinates first and second semester general biology laboratories Previously, he taught general microbiology, virology, the biology of cancer, medical microbiology, and cell biology A bachelor’s degree in cell biology with an emphasis on plant molecular and cellular biology from the University of East Anglia in England led to doctoral studies in cell cycle control, and postdoctoral research on the molecular and biochemical mechanisms of DNA alkylation damage in vitro and in Drosophila melanogaster Currently, he is interested in factors affecting retention and success of incoming first-year students in diverse demographics He lives in Boulder, Colorado, with his two Great Danes, Eddie and Henry.

About the Authors

©Kenneth Mason

©Lesley Howard

©Jonathan Losos

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1 The Science of Biology 1

1.1 The Science of Life 1 1.2 The Nature of Science 4 1.3 An Example of Scientific Inquiry: Darwin and Evolution 8

1.4 Core Concepts in Biology 12

2 The Nature of Molecules and the

Properties of Water 18

2.1 The Nature of Atoms 19 2.2 Elements Found in Living Systems 23 2.3 The Nature of Chemical Bonds 24 2.4 Water: A Vital Compound 26 2.5 Properties of Water 29 2.6 Acids and Bases 30

3 The Chemical Building Blocks of Life 35

3.1 Carbon: The Framework of Biological Molecules 36 3.2 Carbohydrates: Energy Storage and Structural Molecules 40

3.3 Nucleic Acids: Information Molecules 43 3.4 Proteins: Molecules with Diverse Structures and Functions 46

3.5 Lipids: Hydrophobic Molecules 56

4 Cell Structure 63

4.1 Cell Theory 63 4.2 Prokaryotic Cells 67 4.3 Eukaryotic Cells 69

4.4 The Endomembrane System 73 4.5 Mitochondria and Chloroplasts: Cellular

Generators 77 4.6 The Cytoskeleton 79 4.7 Extracellular Structures and Cell Movement 83 4.8 Cell-to-Cell Interactions 86

5 Membranes 92

5.1 The Structure of Membranes 92 5.2 Phospholipids: The Membrane’s Foundation 96 5.3 Proteins: Multifunctional Components 98 5.4 Passive Transport Across Membranes 100 5.5 Active Transport Across Membranes 103 5.6 Bulk Transport by Endocytosis and Exocytosis 106

6 Energy and Metabolism 112

6.1 The Flow of Energy in Living Systems 113 6.2 The Laws of Thermodynamics and Free Energy 114

6.3 ATP: The Energy Currency of Cells 117 6.4 Enzymes: Biological Catalysts 118 6.5 Metabolism: The Chemical Description of Cell Function 122

7 How Cells Harvest Energy 128

7.1 Overview of Respiration 129 7.2 Glycolysis: Splitting Glucose 133 7.3 The Oxidation of Pyruvate Produces Acetyl-CoA 136

7.4 The Citric Acid Cycle 137 7.5 The Electron Transport Chain and Chemiosmosis 140

7.6 Energy Yield of Aerobic Respiration 143 7.7 Regulation of Aerobic Respiration 144 7.8 Oxidation Without O 2 145

7.9 Catabolism of Proteins and Fats 147 7.10 Evolution of Metabolism 149

8 Photosynthesis 154

8.1 Overview of Photosynthesis 154 8.2 The Discovery of Photosynthetic Processes 156

8.3 Pigments 158 8.4 Photosystem Organization 161 8.5 The Light-Dependent Reactions 163 8.6 Carbon Fixation: The Calvin Cycle 167 8.7 Photorespiration 170

©Soames Summerhays/Natural Visions

©Dr Gopal Murti/Science Source

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14 DNA: The Genetic Material 268

14.1 The Nature of the Genetic Material 268 14.2 DNA Structure 271

14.3 Basic Characteristics of DNA Replication 275 14.4 Prokaryotic Replication 278

14.5 Eukaryotic Replication 283 14.6 DNA Repair 285

15 Genes and How They Work 290

15.1 The Nature of Genes 290 15.2 The Genetic Code 293 15.3 Prokaryotic Transcription 296 15.4 Eukaryotic Transcription 299 15.5 Eukaryotic pre-mRNA Splicing 301 15.6 The Structure of tRNA and Ribosomes 303 15.7 The Process of Translation 305

15.8 Summarizing Gene Expression 309 15.9 Mutation: Altered Genes 311

16 Control of Gene Expression 317

16.1 Control of Gene Expression 317 16.2 Regulatory Proteins 318 16.3 Prokaryotic Regulation 321 16.4 Eukaryotic Regulation 325 16.5 Chromatin Structure Affects Gene Expression 328 16.6 Eukaryotic Posttranscriptional Regulation 330 16.7 Protein Degradation 334

17 Biotechnology 340

17.1 Recombinant DNA 340 17.2 Amplifying DNA Using the Polymerase Chain Reaction 345

17.3 Creating, Correcting, and Analyzing Genetic Variation 348

17.4 Constructing and Using Transgenic Organisms 350 17.5 Environmental Applications 354

17.6 Medical Applications 356 17.7 Agricultural Applications 360

18 Genomics 366

18.1 Mapping Genomes 366 18.2 Sequencing Genomes 370 18.3 Genome Projects 373 18.4 Genome Annotation and Databases 374 18.5 Comparative and Functional Genomics 378 18.6 Applications of Genomics 383

19 Cellular Mechanisms of Development 389

19.1 The Process of Development 389 19.2 Cell Division 390

Receptors 186

10 How Cells Divide 194

10.1 Bacterial Cell Division 195

10.2 Eukaryotic Chromosomes 197

10.3 Overview of the Eukaryotic Cell Cycle 200

10.4 Interphase: Preparation for Mitosis 201

10.5 M Phase: Chromosome Segregation and the Division

of Cytoplasmic Contents 203 10.6 Control of the Cell Cycle 206

10.7 Genetics of Cancer 211

Biology

11 Sexual Reproduction and Meiosis 217

11.1 Sexual Reproduction Requires Meiosis 217

11.2 Features of Meiosis 219

11.3 The Process of Meiosis 220

11.4 Summing Up: Meiosis Versus Mitosis 225

12 Patterns of Inheritance 231

12.1 The Mystery of Heredity 231

12.2 Monohybrid Crosses: The Principle of

Segregation 234 12.3 Dihybrid Crosses: The Principle of Independent

Assortment 238 12.4 Probability: Predicting the Results of Crosses 240

12.5 The Testcross: Revealing Unknown Genotypes 241

13.3 Exceptions to the Chromosomal Theory of

Inheritance 255 13.4 Genetic Mapping 255

13.5 Human Genetic Disorders 260

©Steven P Lynch

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19.3 Cell Differentiation 392 19.4 Nuclear Reprogramming 397 19.5 Pattern Formation 400 19.6 Evolution of Pattern Formation 406 19.7 Morphogenesis 409

Part IV Evolution

20 Genes Within Populations 416

20.1 Genetic Variation and Evolution 416 20.2 Changes in Allele Frequency 418 20.3 Five Agents of Evolutionary Change 420 20.4 Quantifying Natural Selection 425 20.5 Reproductive Strategies 426 20.6 Natural Selection’s Role in Maintaining Variation 430

20.7 Selection Acting on Traits Affected by Multiple Genes 432

20.8 Experimental Studies of Natural Selection 434 20.9 Interactions Among Evolutionary Forces 436 20.10 The Limits of Selection 437

21 The Evidence for Evolution 443

21.1 The Beaks of Darwin’s Finches: Evidence of Natural Selection 444

21.2 Peppered Moths and Industrial Melanism: More Evidence

of Selection 446 21.3 Artificial Selection: Human-Initiated Change 448

21.4 Fossil Evidence of Evolution 450 21.5 Anatomical Evidence for Evolution 454 21.6 Convergent Evolution and the Biogeographical Record 456

21.7 Darwin’s Critics 458

22 The Origin of Species 463

22.1 The Nature of Species and the Biological Species Concept 463

22.2 Natural Selection and Reproductive Isolation 468 22.3 The Role of Genetic Drift and Natural Selection in Speciation 469

22.4 The Geography of Speciation 471 22.5 Adaptive Radiation and Biological Diversity 473 22.6 The Pace of Evolution 478

22.7 Speciation and Extinction Through Time 479

23 Systematics, Phylogenies, and

Comparative Biology 484

23.1 Systematics 484 23.2 Cladistics 486

23.3 Systematics and Classification 489 23.4 Phylogenetics and Comparative Biology 493 23.5 Phylogenetics and Disease Evolution 499

24 Genome Evolution 504

24.1 Comparative Genomics 504 24.2 Genome Size 508

24.3 Evolution Within Genomes 511 24.4 Gene Function and Expression Patterns 515 24.5 Applying Comparative Genomics 516

on Earth

25 The Origin and Diversity

of Life 523

25.1 Deep Time 525 25.2 Origins of Life 525 25.3 Evidence for Early Life 528 25.4 Earth’s Changing System 530 25.5 Ever-Changing Life on Earth 531

26 Viruses 537

26.1 The Nature of Viruses 538 26.2 Viral Diversity 542 26.3 Bacteriophage: Bacterial Viruses 544 26.4 Viral Diseases of Humans 546 26.5 Prions and Viroids: Infectious Subviral Particles 552

27 Prokaryotes 557

27.1 Prokaryotic Diversity 558 27.2 Prokaryotic Cell Structure 562 27.3 Prokaryotic Genetics 567 27.4 The Metabolic Diversity of Prokaryotes 571 27.5 Microbial Ecology 573

27.6 Bacterial Diseases of Humans 575

©tamoncity/Shutterstock

©Jeff Hunter/Getty Images

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29 Seedless Plants 608

29.1 Origin of Land Plants 608

29.2 Bryophytes Have a Dominant Gametophyte

Generation 611 29.3 Tracheophytes Have a Dominant Sporophyte

Generation 613 29.4 Lycophytes Diverged from the Main Lineage

of Vascular Plants 616 29.5 Pterophytes Are the Ferns and Their

Relatives 617

30 Seed Plants 623

30.1 The Evolution of Seed Plants 623

30.2 Gymnosperms: Plants with “Naked Seeds” 624

30.3 Angiosperms: The Flowering Plants 628

31.4 Fungal Parasites and Pathogens 650

31.5 Basidiomycota: The Club (Basidium)

Fungi 652 31.6 Ascomycota: The Sac (Ascus) Fungi 654

31.7 Glomeromycota: Asexual Plant Symbionts 656

31.8 Zygomycota: Zygote-Producing Fungi 656

31.9 Chytridiomycota and Relatives: Fungi with

Zoospores 658 31.10 Microsporidia: Unicellular Parasites 659

32 Animal Diversity and the Evolution

of Body Plans 664

32.1 Some General Features of Animals 664

32.2 Evolution of the Animal Body Plan 666

32.3 Animal Phylogeny 670

32.4 Parazoa: Animals That Lack Specialized

Tissues 674 32.5 Eumetazoa: Animals with True Tissues 677

33.9 Arthropods (Arthropoda) 707

34 Deuterostomes 720

34.1 Echinoderms 721 34.2 Chordates 723 34.3 Nonvertebrate Chordates 725 34.4 Vertebrate Chordates 726 34.5 Fishes 728

34.6 Amphibians 733 34.7 Reptiles 737 34.8 Birds 742 34.9 Mammals 746 34.10 Evolution of the Primates 751

36 Transport in Plants 788

36.1 Transport Mechanisms 789 36.2 Water and Mineral Absorption 792 36.3 Xylem Transport 795

36.4 Rate of Transpiration 797 36.5 Water-Stress Responses 799 36.6 Phloem Transport 801

37 Plant Nutrition and Soils 807

37.1 Soils: The Substrates on Which Plants Depend 807 37.2 Plant Nutrients 811

37.3 Special Nutritional Strategies 813 37.4 Carbon–Nitrogen Balance and Global Change 816 37.5 Phytoremediation 819

38 Plant Defense Responses 825

38.1 Physical Defenses 825 38.2 Chemical Defenses 827 38.3 Animals That Protect Plants 831 38.4 Systemic Responses to Invaders 832

39 Sensory Systems in Plants 838

39.1 Responses to Light 838 39.2 Responses to Gravity 843 39.3 Responses to Mechanical Stimuli 845

©Susan Singer

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39.4 Responses to Water and Temperature 847 39.5 Hormones and Sensory Systems 849

40 Plant Reproduction 866

40.1 Reproductive Development 867 40.2 Making Flowers 869

40.3 Structure and Evolution of Flowers 874 40.4 Pollination and Fertilization 877 40.5 Embryo Development 882 40.6 Germination 888 40.7 Asexual Reproduction 891 40.8 Plant Life Spans 893

Part VII Animal Form and

41.5 Nerve Tissue 909 41.6 Overview of Vertebrate Organ Systems 910 41.7 Homeostasis 913

41.8 Regulating Body Temperature 915

42 The Nervous System 924

42.1 Nervous System Organization 925 42.2 The Mechanism of Nerve Impulse Transmission 928 42.3 Synapses: Where Neurons Communicate with Other Cells 933

42.4 The Central Nervous System: Brain and Spinal Cord 939

42.5 The Peripheral Nervous System: Spinal and Cranial Nerves 946

43 Sensory Systems 955

43.1 Overview of Sensory Receptors 956 43.2 Thermoreceptors, Nociceptors, and Electromagnetic Receptors: Temperature, Pain, and Magnetic Fields 958

43.3 Mechanoreceptors I: Touch, Pressure, and Body Position 959

43.4 Mechanoreceptors II: Hearing, Vibration, and Balance 961

43.5 Chemoreceptors: Taste, Smell, and pH 967 43.6 Vision 969

43.7 Evolution and Development of Eyes 975

44 The Endocrine System 982

44.1 Regulation of Body Processes by Chemical Messengers 983

44.2 Overview of Hormone Action 988 44.3 The Pituitary and Hypothalamus: The Body’s Control Centers 991

44.4 The Major Peripheral Endocrine Glands 996 44.5 Other Hormones and Their Effects 1000

45 The Musculoskeletal System 1006

45.1 Types of Skeletal Systems 1007 45.2 A Closer Look at Bone 1009 45.3 Joints 1012

45.4 Muscle Contraction 1013 45.5 Vertebrate Skeleton Evolution and Modes

of Locomotion 1020

46 The Digestive System 1026

46.1 Types of Digestive Systems 1027 46.2 The Mouth and Teeth: Food Capture and Bulk Processing 1029

46.3 The Esophagus and the Stomach: The Early Stages

of Digestion 1030 46.4 The Intestines: Breakdown, Absorption, and Elimination 1032

46.5 Accessory Organ Function 1035 46.6 Neural and Hormonal Regulation of the Digestive Tract 1037

46.7 Food Energy, Energy Expenditure, and Essential Nutrients 1038

46.8 Variations in Vertebrate Digestive Systems 1042

47 The Respiratory System 1047

47.1 Gas Exchange Across Respiratory Surfaces 1048 47.2 Gills, Cutaneous Respiration, and Tracheal Systems 1049

47.3 Lungs 1052 47.4 Structures, Mechanisms, and Control of Ventilation

in Mammals 1055 47.5 Transport of Gases in Body Fluids 1059

48 The Circulatory System 1066

48.1 Invertebrate Circulatory Systems 1066 48.2 The Components of Vertebrate Blood 1068

48.3 Vertebrate Circulatory Systems 1071 48.4 Cardiac Cycle, Electrical Conduction, ECG, and Cardiac Output 1074

48.5 Blood Pressure and Blood Vessels 1078

49 Osmotic Regulation and the Urinary System 1088

49.1 Osmolarity and Osmotic Balance 1088 49.2 Nitrogenous Wastes: Ammonia, Urea, and Uric Acid 1090

©Dr Roger C Wagner, Professor Emeritus of

Blologlcal Sciences, University of Delaware

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49.3 Osmoregulatory Organs 1091

49.4 Evolution of the Vertebrate Kidney 1093

49.5 The Mammalian Kidney 1095

49.6 Hormonal Control of Osmoregulatory

50.4 Humoral Immunity and Antibody Production 1119

50.5 Autoimmunity and Hypersensitivity 1125

50.6 Antibodies in Medical Treatment and

Diagnosis 1127 50.7 Pathogens That Evade the Immune System 1130

51 The Reproductive System 1135

51.1 Animal Reproductive Strategies 1135

51.2 Vertebrate Fertilization and Development 1138

51.3 Structure and Function of the Human Male

Reproductive System 1142 51.4 Structure and Function of the Human Female

Reproductive System 1146 51.5 Contraception and Infertility Treatments 1150

53.1 The Natural History of Behavior 1189

53.2 Nerve Cells, Neurotransmitters, Hormones, and

Behavior 1190 53.3 Behavioral Genetics 1191

54 Ecology of Individuals and Populations 1218

54.1 The Environmental Challenges 1218 54.2 Populations: Groups of a Single Species in One Place 1221

54.3 Population Demography and Dynamics 1224 54.4 Life History and the Cost of Reproduction 1227 54.5 Environmental Limits to Population Growth 1230 54.6 Factors That Regulate Populations 1232

54.7 Human Population Growth 1235

56 Dynamics of Ecosystems 1265

56.1 Biogeochemical Cycles 1266 56.2 The Flow of Energy in Ecosystems 1272 56.3 Trophic-Level Interactions 1277 56.4 Biodiversity and Ecosystem Stability 1281 56.5 Island Biogeography 1284

57 The Biosphere and Human Impacts 1289

57.1 Ecosystem Effects of Sun, Wind, and Water 1289 57.2 Earth’s Biomes 1294

57.3 Freshwater Habitats 1297 57.4 Marine Habitats 1300 57.5 Human Impacts on the Biosphere: Pollution and Resource Depletion 1304

57.6 Human Impacts on the Biosphere: Climate Change 1310

58.5 Approaches for Preserving Endangered Species and Ecosystems 1339

Appendix A Glossary G-1 Index I-1

©K Ammann/Bruce Coleman Inc./Photoshot

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Committed to Excellence

With the new 12th edition, Raven and Johnson’s Biology continues

the momentum built over the last four editions We continue to

pro-vide an unmatched comprehensive text fully integrated with a

con-tinually evolving, state-of-the-art digital environment We have

used this revision to recommit ourselves to our roots as the majors

biology text that best integrates evolution throughout We have

added material emphasizing the relevance of evolution throughout

the ecology section, not only in all four ecology chapters, but also

in the chapters on behavior and conservation biology In the animal

form and function section we have done extensive revision to

mod-ernize, and to emphasize evolution in the context of physiology

Important contributions to this effort came from Dr Charles Welsh

(Duquesne University), who provided his knowledge and

experi-ence to this important section We have also moved the examples

and insights from the chapter devoted to the evolution of

develop-ment to place them into the appropriate context throughout the

book This emphasizes the importance of evolution and

develop-ment by continually providing examples rather than gathering them

together in a single chapter

We have also renewed our commitment to the ideas set forth

in the Vision and Change report from the AAAS, which provides a

framework for modern undergraduate biology education This

re-port will have been with us for a decade coincident with our 12th

edition One important idea articulated by Vision and Change was

an emphasis on core concepts One of the key differences between

the way an expert organizes information in their brain compared to

a novice is that the expert has a conceptual framework in place to

incorporate new information We have designed the new

Connect-ing the Concepts feature to address this disparity We emphasize

core concepts in each chapter, then at the end of the chapter show

how these can be used to build a conceptual framework, and

en-courage the student to begin building their own At the end of each

part of the book we expand this to show how core concepts are

interrelated and how a much larger conceptual framework is

constructed

One unanticipated consequence of the Vision and Change movement was how publishers chasing new approaches would

produce books so “feature-laden” as to be virtually unreadable by

the average student We have not abandoned the idea that

narra-tive flow is important, even in a science textbook While we

include a variety of features to improve student learning, they are

integrated into the text and not at the expense of the concise,

ac-cessible, and engaging writing style we are known for We

main-tain the clear emphasis on evolution and scientific inquiry that

have made this a leading textbook of choice for majors biology

students

Faculty want textbooks that emphasize student-centered proaches, and core concepts for the biological sciences As a team,

ap-we continually strive to improve the text by integrating the latest

cognitive and best practices research with methods that are known

to positively affect learning We emphasize scientific inquiry,

in-cluding an increased quantitative emphasis in the Scientific

Thinking figures Our text continues to be a leader with an organization that emphasizes important biological concepts, while keeping the student engaged with learning outcomes that allow as-sessment of progress in understanding these concepts An inquiry-based approach with robust, adaptive tools for discovery and assessment in both text and digital resources provides the intellec-tual challenge needed to promote student critical thinking and en-sure academic success

We continue to use our digital environment in the revision of

Biology A major strength of both text and digital resources is ment across multiple levels of Bloom’s taxonomy that develops critical-thinking and problem-solving skills in addition to com-prehensive factual knowledge

assess-McGraw-Hill Education’s Connect® platform offers a powerful suite of online tools that are linked to the text and in-cludes new quantitative assessment tools We now have avail-able interactive exercises that use graphical data, controlled by the student, to engage them in actively exploring quantitative aspects of biology Our adaptive learning system helps students learn faster, study efficiently, and retain more knowledge of key concepts

The 12th edition continues to employ the aesthetically

stunning art program that the Raven and Johnson Biology text

is known for Complex topics are represented clearly and cinctly, helping students to build the mental models needed to understanding biology

suc-We continue to incorporate student usage data and input, rived from thousands of our SmartBook® users SmartBook “heat maps” provided a quick visual snapshot of chapter usage data and the relative difficulty students experienced in mastering the con-tent This “heat-mapping” technology is unique in the industry, and allows direct editing of difficult areas, or problem areas for students

de-■ If the data indicated that the subject was more difficult than other parts of the chapter, as evidenced by a high proportion

of students responding incorrectly to the probes, we revised

or reorganized the content to be as clear and illustrative as possible

■ In other cases, if one or more of the SmartBook probes for a section was not as clear as it might be or did not appropriately reflect the content, we edited the probe, rather than the text

We’re excited about the 12th edition of this quality textbook providing a learning path for a new generation of students All of

us have extensive experience teaching undergraduate biology, and we’ve used this knowledge as a guide in producing a text that is up

to date, beautifully illustrated, and pedagogically sound for the dent We are also excited about the continually evolving digital environment that provides unique and engaging learning environ-ment for modern students We’ve worked hard to provide clear ex-plicit learning outcomes, and more closely integrate the text with

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stu-its media support materials to provide instructors with an excellent

complement to their teaching

Ken Mason, Jonathan Losos, Tod Duncan

Cutting Edge Science

Changes to the 12th Edition

Part I: The Molecular Basis of Life

Chapter 1—New section added that elaborates on the core

concepts and prepares the student for the use of the Connecting

the Concepts feature

Chapter 2—Edited for clarity, especially regarding atomic

structure and the periodic table

Chapter 3—Edited for clarity especially regarding the structure

of nucleotides, the role of ATP in cells, and secondary structure

in proteins

Part II: Biology of the Cell

Chapter 4—The section on the endomembrane system has been

completely rewritten This includes new material on lipid

droplets Material on adhesive junctions has been rewritten to

give a more evolutionary perspective

Chapter 5—New material on proteins that can alter membrane

structure has been added This provides information on how the

different cellular membranes can have different structures Figure

on Na+/K+ pump was redone to address errors in mechanism

Material on diffusion and facilitated diffusion was rewritten

Chapter 6—The material on free energy and chemical

reac-tions was completely rewritten, including redoing the figures

These changes significantly improve clarity and accuracy

Material on the role of ATP in cells was rewritten for clarity

Discussions of energy throughout the chapter were rewritten to

improve clarity and accuracy of chemical concepts

Chapter 7—The nature and action of cofactors in redox

reactions and the role of ATP in cells were improved

Chapter 8—The nature and structure of photosystems was

rewritten for clarity and accuracy

Chapter 10—The section on chromosome structure was

completely rewritten to reflect new data and views of this

important topic The material on cancer was expanded and

updated, producing a new section “Genetics of Cancer.” This

contains significant new information and pulls together

material on cancer from this chapter and others

Part III: Genetic and Molecular Biology

The overall organization of this section remains the same We

have retained the split of transmission genetics into two chapters

as it has proved successful for students

Chapter 11—Edited for clarity and readability for the student,

especially regarding the events of meiosis I

Chapter 12—The material on extensions to Mendel was

rewritten for clarity and accuracy

Chapter 13—The material on analyzing and mapping genetic

variation in humans was updated and rewritten The section on human genetic disorders was completely rewritten to reflect new information, and to make more accessible for the student A new figure on imprinting in mouse was added to clarify this important and difficult concept

Chapter 14—The material on eukaryotic DNA replication was

rewritten and updated Particular emphasis was placed on the evolution of DNA replication The section on DNA repair was rewritten and updated and information on mismatch repair was added

Chapter 15—Content on process of transcription was rewritten

to reflect new data on elongation machinery New data on alternative splicing was included, along with information on the integration of RNA modification during transcription The section on the nature of mutations was rewritten and includes latest data on human mutation rates

Chapter 16—Overview of control of eukaryotic transcription

was rewritten to reflect modern views Continued updating of the material on chromatin structure and the control of gene expression Material on control of gene expression at the level

of transcription was updated

Chapter 18—New section added on the 1000 Genomes project

to illustrate how fast information on genetic diversity is mulating The material on the wheat genome was updated, which provides both new information and approaches to complex genomes

accu-Chapter 19—Added a new section on the evolution of pattern

formation using new material and material from chapter 25

This consolidates material on this subject, and provides a clear vision for the student

Part IV: Evolution

Chapter 20—The topic of sexual selection was moved into this

chapter from the Behavioral Biology chapter Some material on Lamarck was eliminated, natural selection was explicitly defined, information on snp variation in humans and other animals was added New examples of pleiotropy were added, and new data on how the speed of racehorses has not changed through time were added along with a revised figure A new section was added on the role of sensory exploitation as a mechanism for traits to evolve under sexual selection

Chapter 21—A number of points were updated and an

exam-ple of vestigial traits involving the toenails of manatees was added

Trang 14

Chapter 23—The figure on the evolution of feathers in

dino-saurs was updated to incorporate new paleontological findings

Discussion of HIV evolution and other points were also revised

in light of new science

Chapter 24—Updated material on comparative genomics of

vertebrates New data on Neanderthal and Denisovan genomes

have been added Presentation of genes unique to humans has

been updated and edited for clarity

Note: Evolution of Development (chapter 25 in the 11th edition)

was eliminated and material moved to other chapters, placing the

topic of evolution of development into the appropriate context

This reflects the view that evolution and development are now so

clearly intertwined with all of biology that setting off the material

in a separate chapter no longer made sense

Part V: Diversity of Life on Earth

Chapter 26—This chapter has been largely rewritten and now

includes material on viral diversity, classification, metagenomics,

and taxonomy The latter part of the chapter now focuses on viruses

of medical importance to promote student engagement and interest

Chapter 27—This chapter has been largely rewritten In addition

to the traditional discussion of prokaryotic structure and function,

and taxonomy, there is new emphasis placed on microbial

ecology and medical microbiology with relevant examples

Chapter 31—The chapter has been rewritten for clarity The

chapter has also been reordered to bring material most relevant to

society to the front of the chapter The reorganization includes

expanding and moving the fungal ecology up earlier in the chapter,

as well as expanding and moving the fungal parasites and

patho-gens up earlier in the chapter The chapter now ends with the

coverage of fungal classification

Chapter 32—Aspects of taxonomy and natural history were

updated in line with new findings

Chapter 33—The presentation of taxonomic relationships was

revised as a result of new findings based primarily on molecular

phylogenetic studies, specifically with regards to

Platyhelmin-thes, lophotrochozoans (formerly Spiralia) and a few others

New natural history information was included

Chapter 34—The discussion of the evolutionary history of

vertebrates was substantially revised, especially the sections on

lobe-finned fishes/early tetrapods/early amniotes (emphasizing

now those terms, rather than referring to all of the early diverging

lineages as amphibians or reptiles) Also, the terminology about

human evolution was revised to acknowledge the new meaning of

“hominin” and “hominid.” A new paragraph on Homo naledi was

added to discuss recent discoveries

Part VI: Plant Form and Function

There have been no major changes in the plant form and function

chapters There has been overall editing for readability and

responding to recommendations by reviewers and users of the 11th edition

Part VII: Animal Form and Function

Charles Welsh of Duquesne University, brought his expertise

in animal anatomy and physiology as a Contributor to the Animal Form and Function Part in the 12th edition, placing greater emphasis on evolutionary aspects of animal biology

Chapter 41—The discussion of the evolution of tissues in

invertebrates and vertebrates was expanded, including the addition of a phylogeny and an image of cnidarian tissues

Chapter 42—The graph of an action potential was revised

and improved Discussions and images of glial cells and cranial nerves were added

Chapter 43—The chapter was revised and reorganized

with regards to the general senses The evolution of eyes material found in chapter 25 in the 11th edition was moved

to this chapter with a revised phylogeny added The illustration depicting the evolution of the inner ear has been revised to make it more clear, concise, and informative

Chapter 44—Section 44.2 was formerly organized as action

of lipophilic vs hydrophilic hormones This has now been reorganized to be a complete overview of how hormones work This organization should improve clarity for students

Chapter 45—The chapter was extensively revised This

included the addition of images for the human skeleton, ossification, osteoporosis, invertebrate muscle, comparative anatomy of flying vertebrates, and a new phylogeny that reveals the evolution of various vertebrate skeletal characters

Chapter 46—The structure of the latter half this chapter

was completely reorganized for better conceptual flow

Chapter 47—The images for the bicarbonate buffering

system and the mechanics of breathing have been revised

The discussion of lung volumes and capacities was

expand-ed with the addition of an accompanying figure

Chapter 48—The chapter was reorganized and extensively

revised Invertebrate circulatory systems is now the first section in the chapter The sections on Cardiac Cycle, ECG, Electrical Conduction, and Cardiac Output have been reorga-nized and revised The discussions of blood vessels and blood pressure are now in the same section The phylogeny of the evolution of vertebrate hearts has been revised

Chapter 50—Material on innate immunity was updated

and rewritten for clarity The coverage on effects of AIDS was also updated to reflect new information

Chapter 51—A discussion of some select invertebrate

repro-ductive strategies has been added, with accompanying images

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Chapter 52—A section detailing the classic experiments

regarding pattern formation in chick limb buds has been added

This includes a discussion of AER, ZPA, FGF, Hox genes, and

Shh The material on gene regulation from chapter 25 in the

11th edition has also been added

Part VIII: Ecology and Behavior

Chapter 53—Stronger emphasis on phylogenetic and

evolution-ary perspectives was added throughout the chapter, including a

new section on evolution and behavior

Chapter 54—Human population trends and other timely data were

updated to stay current An evolutionary perspective on population

adaptation was added to the beginning of the chapter

Chapter 55—An evolutionary perspective was added in several

places

Chapter 56—New material on the impact of anthropogenic

changes on nutrient cycling was added An evolutionary

perspec-tive to discussion of the species-area relationship was incorporated

Chapter 57—Evolution was discussed more thoroughly in the

section on microclimate adaptation during adaptive radiation

All of the data on biosphere impacts of humans were updated to

stay current

Chapter 58—The chapter was substantially revised, including

much new discussion of the relevance of evolution to

conserva-tion biology, including the role of natural selecconserva-tion, the

impor-tance of phylogenetic perspectives, and how speciation can lead

to biodiversity hotspots

A Note From the Authors

A revision of this scope relies on the talents and efforts of many

people working behind the scenes and we have benefited greatly

from their assistance

Dr Charles Welsh made significant contributions to the Animal

Form and Function section He updated them to provide a more

modern perspective, and added new examples

Beth Bulger was the copyeditor for this edition She has bored many hours and always improves the clarity and consis-tency of the text She has made significant contributions to the quality of the final product

la-We were fortunate to work again with MPS to update the art program and improve the layout of the pages Our close collabora-tion resulted in a text that is pedagogically effective as well as more beautiful than any other biology text on the market

We have the continued support of an excellent team at McGraw-Hill Education Andrew Urban, preceded by Justin

Wyatt, the portfolio managers for Biology have been steady

leaders during a time of change Senior Product Developer Liz Sievers, provided support in so many ways it would be impossi-ble to name them all Kelly Hart, content project manager, and David Hash, designer, ensured our text was on time and elegantly designed Kelly Brown, senior marketing manager, is always a sounding board for more than just marketing, and many more people behind the scenes have all contributed to the success of our text This includes the digital team, whom we owe a great deal for their efforts to continue improving our Connect assessment tools

Throughout this edition we have had the support of spouses and families, who have seen less of us than they might have liked because of the pressures of getting this revision complet-

ed They have adapted to the many hours this book draws us away from them, and, even more than us, looked forward to its completion

In the end, the people we owe the most are the generations of students who have used the many editions of this text They have taught us at least as much as we have taught them, and their ques-tions and suggestions continue to improve the text and supple-mentary materials

Finally, we need to thank instructors from across the country who are continually sharing their knowledge and experience with

us through market feedback and symposia The feedback we ceived shaped this edition All of these people took time to share

re-their ideas and opinions to help us build a better edition of Biology

for the next generation of introductory biology students, and they have our heartfelt thanks

Reviewers for Biology, 12th edition

Carron Bryant East Mississippi Community

Mark Levenstein University of Wisconsin, Platteville

Cindy Malone California State University Northridge

David McClellan University of Arkansas Fort Smith

Shilpi Paul SUNY College at Old Westbury Crima Pogge City College of San Francisco

Josephine Rodriguez The University of Virginia’s College at Wise Connie Rye East Mississippi Community College

Devinder Sandhu USDA—Agricultural Research Service

Ken Saville Albion College Steven Shell The University of Virginia’s College at Wise

Walter Smith The University of Virginia’s College at Wise

Qiang Sun University of Wisconsin, Stevens Point

Christopher Vitek University of Texas Rio Grande Valley

D Alexander Wait Missouri State University Maureen Walter Florida International University

Darla Wise Concord University

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Scientific Thinking Figures

Key illustrations in every chapter highlight how the frontiers

of knowledge are pushed forward by a combination of

esis and experimentation These figures begin with a

hypoth-esis, then show how it makes explicit predictions, tests these

by experiment and finally demonstrates what conclusions can

be drawn, and where this leads Scientific Thinking figures

provide a consistent framework to guide the student in the

logic of scientific inquiry Each illustration concludes with

open-ended questions to promote scientific inquiry

Hypothesis: The plasma membrane is fluid, not rigid.

Prediction: If the membrane is fluid, membrane proteins may

diffuse laterally.

Test: Fuse mouse and human cells, then observe the distribution

of membrane proteins over time by labeling specific mouse and human proteins.

Result: Over time, hybrid cells show increasingly intermixed proteins.

Conclusion: At least some membrane proteins can diffuse laterally in

the membrane.

Further Experiments: Can you think of any other explanation for

these observations? What if newly synthesized proteins were inserted into the membrane during the experiment? How could you use this basic experimental design to rule out this or other possible explanations?

SCIENTIFIC THINKING

Mouse cell

Human cell

Fuse cells

Intermixed membrane proteins

Allow time for mixing to occur

Data Analysis Questions

It’s not enough that students learn concepts and memorize scientific facts, a biologist needs to analyze data and apply that knowledge Data Analysis questions inserted throughout the text challenge students to analyze data and Interpret experimental results, which shows a deeper level of understanding

Inquiry Questions

Questions that challenge students to think about and engage in what they are reading at a more sophisticated level

Preparing Students for the Future

Developing Critical Thinking with the Help of

Figure 5.5 Test of membrane fluidity.

?

24 26 28 30 32

Air Temperature (°C)

24 26 28 30 32

open habitat shaded forest

Figure 55.3 Behavioral adaptation. In open habitats, the

Puerto Rican crested lizard, Anolis cristatellus, maintains a relatively

constant temperature by seeking out and basking in patches of sunlight; as a result, it can maintain a relatively high temperature even when the air is cool In contrast, in shaded forests, this behavior is not possible, and the lizard’s body temperature conforms to that of its surroundings

(inset) ©Melissa Losos

Inquiry question When given the opportunity, lizards regulate their body temperature to maintain a temperature optimal for physiological functioning Would lizards in open habitats exhibit different escape behaviors from those of lizards in shaded forest?

Data analysis Can the slope of the line tell us something about the behavior of the lizard?

Trang 17

Soil properties determine plant nutrient availability Life is subject

to chemical and physical laws

Living systems transform energy & matter

Plants can detoxify certain contaminated environments

• Positively charged soil

nutrients must be actively

transported into roots due

to their sequestration by

anionic soil particles.

• Porous soils leach water

rapidly and can contribute

to water stress.

• The chemical properties of

clay make it adsorb water

and minerals tightly.

• The water potential of the

soil affects the transport of

minerals into the root.

• Low soil pH can cause toxic

aluminum to leach from

rocks.

• Salt accumulation in soil

can affect soil water

potential and cause loss of

plant cell turgor.

C O N N E C T I N G T H E C O N C E P T S

This feature is intended to give you practice in organizing information using core concepts We use a metaphor of gears and cogs to represent a conceptual

hierarchy with each core concept represented as a gear Secondary concepts are the cogs, and tertiary concepts, which are particular examples from the chapter,

are presented as a list of bulleted points Using the completed conceptual unit as a guide, build from material in the chapter a list of tertiary concepts that

support the open secondary concept.

Connecting the Concepts

There are two new but related features in Biology, 12th edition

that help students build a conceptual framework into which they

can insert new knowledge The Connecting the Concepts feature

at the end of the chapters identifies core concepts that are

related to material in the chapter The conceptual framework

begins with a core concept that is represented by a gear icon

Examples from the chapter that relate to the core concept are

secondary concepts that are placed on the cogs Each cog

contains a list of observations from the chapter that connects the secondary concept to the core concept

At the chapter level:

The Connecting the Concept shows the student a completed concept (core concept, secondary concept, list of observations)

A second cog or gear is presented that lacks the list of tions The student is challenged to identify examples from the chapter that demonstrate how the secondary concept is related

observa-to the core concept

At the Part level:

As valuable as that exercise is, the full understanding of a

conceptual framework and how that helps students see the

connections to core concepts is when the chapter-ending

Connecting the Concepts are pulled together This happens at

the Part level, which themselves present a higher level to the

conceptual framework When these are built, students see how topics that appear unrelated fit into the conceptual framework

of the core concepts Once students begin to see these tions, the topics and information in biology make

connec-more sense

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Connecting the Concepts Part VI Plant Form and Function

Vascular plants are comprised of roots and shoots, which in turn are made of three principal tissue types Each of these tissues has distinct cell types that express the genes needed to produce the proteins necessary for their specialized functions Plants move fluids using differ- ences in solute concentration and pressure Plant form is often an evolutionary compromise between competing needs such as maximizing the surface area of leaves for photosynthesis while minimizing water loss when exchanges gases The reproductive structures of plants are organized into flowers that have evolved to facilitate the dissemination of genetic information.

Living systems depend on information transactions

• Positively charged soil nutrients must be actively transported into roots due to their sequestration by anionic soil particles.

• Porous soils leach water rapidly and can contribute to water stress

• The chemical properties of clay make it adsorb water and minerals tightly

• The water potential of the soil affects the transport of minerals into the root

• Low soil pH can cause toxic aluminum to leach from rocks.

• Salt accumulation in soil can affect soil water potential and cause loss of plant cell turgor.

• Light can be perceived by plant cell receptors such as P fr

• Signal transduction pathways communicate information received in light signals to plant response mechanisms.

• Plants can respond to perceived light with changes in gene expression.

• Differences in received light wavelength can cause specific plant growth responses.

• The environment can signal seeds to germinate using light of specific wavelengths.

• Light containing blue wavelengths can signal phototropic responses.

• Some plants can change behavior based on the day/night cycle.

• Gravitational fields can trigger directional growth responses.

• Some plants can respond to touch.

• Gibberellins, a family of growth hormones, can be produced by bacteria infecting certain plants’

roots and influence plant growth.

• Allelopathy is a form of signaling where one plant releases compounds that inhibit seed germination or the growth of neighboring plants.

• Toxins produced by plants communicate to potential predators that the plant is not safe to eat.

• Chemical signals can modulate the behaviors of insects that protect plants from predation.

• Chemicals released by plants as a wound response can attract insects

to defend the plant against herbivores.

• The plant hormone jasmonic acid transduces long distance wound response signals in plant bodies.

• The cohesion and adhesion of water molecules allows forces generated by transpiration to move water great distances in plants

• The rate of osmosis limits water movement into roots, but is accelerated

by facilitated diffusion through rins

aquapo-• The combined effects of solute potential and pressure potential determine the direction of water movement into and out

of plant cells

• Water transport from roots to shoots is driven by a gradient of water potential with lowest values in the leaves.

• Chemical and physical properties of membranes and cell walls restrict the movement of solutes through the plant

• Leaves are arranged on stems to maximize light capture

• Stems may have secondary growth to provide support to the plant body

• Axillary buds produced by the shoot apical meristem allow leaves or flowers to be produced

on the stem

• Horizontal stems allow a plant to spread laterally above ground.

• Tubers can be packed with starch for storage purposes.

• Flattened stems of some cacti capture light energy for photosynthesis.

determine plant nutrient availability

Physics and chemistry dictate movement of water into and around the plant

Life is subject

to chemical and physical laws

Information can be communicated

in chemical ways

non-Signaling plant health

Stems and modified stems carry out a variety of functions

Structure determines function

• Gametes are produced in the gametophytes of flowers

• The calyx protects the budding flower

• The petals collectively form the corolla and their colors attract animal pollinators

• Wind-pollinated plants don’t have elaborate corollas because they don’t need to attract pollinators.

• The long stamens make pollen more accessible to animal pollinators or wind.

• The carpel houses the female reproductive structures with the elongated style being more accessible to pollinators or pollen carried by the wind

Flowers adapted for reproduction

Preparing Students for the Future xvii

Connecting the Concepts Part VI Plant Form and Function

Vascular plants are comprised of roots and shoots, which in turn are made of three principal tissue types Each of these tissues has distinct cell types that express the genes needed to produce the proteins necessary for their specialized functions Plants move fluids using differ- ences in solute concentration and pressure Plant form is often an evolutionary compromise between competing needs such as maximizing the surface area of leaves for photosynthesis while minimizing water loss when exchanges gases The reproductive structures of plants are organized into flowers that have evolved to facilitate the dissemination of genetic information.

Living systems depend on information transactions

• Positively charged soil nutrients must be actively transported into roots due to their sequestration by anionic soil particles.

• Porous soils leach water rapidly and can contribute to water stress

• The chemical properties of clay make it adsorb water and minerals tightly

• The water potential of the soil affects the transport of minerals into the root

• Low soil pH can cause toxic aluminum to leach from rocks.

• Salt accumulation in soil can affect soil water potential and cause loss of plant cell turgor.

• Light can be perceived by plant cell receptors such as P fr

• Signal transduction pathways communicate information received in light signals to plant response mechanisms.

• Plants can respond to perceived light with changes in gene expression.

• Differences in received light wavelength can cause specific plant growth responses.

• The environment can signal seeds to germinate using light of specific wavelengths.

• Light containing blue wavelengths can signal phototropic responses.

• Some plants can change behavior based on the day/night cycle.

• Gravitational fields can trigger directional growth responses.

• Some plants can respond to touch.

• Gibberellins, a family of growth hormones, can be produced by bacteria infecting certain plants’

roots and influence plant growth.

• Allelopathy is a form of signaling where one plant releases compounds that inhibit seed germination or the growth of neighboring plants.

• Toxins produced by plants communicate to potential predators that the plant is not safe to eat.

• Chemical signals can modulate the behaviors of insects that protect plants from predation.

• Chemicals released by plants as a wound response can attract insects

to defend the plant against herbivores.

• The plant hormone jasmonic acid transduces long distance wound response signals in plant bodies.

• The cohesion and adhesion of water molecules allows forces generated by transpiration to move water great distances in plants

• The rate of osmosis limits water movement into roots, but is accelerated

by facilitated diffusion through rins

aquapo-• The combined effects of solute potential and pressure potential determine the direction of water movement into and out

of plant cells

• Water transport from roots to shoots is driven by a gradient of water potential with lowest values in the leaves.

• Chemical and physical properties of membranes and cell walls restrict the movement of solutes through the plant

• Leaves are arranged on stems to maximize light capture

• Stems may have secondary growth to provide support to the plant body

• Axillary buds produced by the shoot apical meristem allow leaves or flowers to be produced

on the stem

• Horizontal stems allow a plant to spread laterally above ground.

• Tubers can be packed with starch for storage purposes.

• Flattened stems of some cacti capture light energy for photosynthesis.

properties nutrient availability

Physics and chemistry dictate movement of water into and around the plant Life is subject

to chemical and physical laws

Information can be communicated

in chemical ways

non-Signaling mediates plant health

Stems and modified stems carry out a variety of functions

Structure determines function

• Gametes are produced in the gametophytes of flowers

• The calyx protects the budding flower

• The petals collectively form the corolla and their colors attract animal pollinators

• Wind-pollinated plants don’t have elaborate corollas because they don’t need to attract pollinators.

• The long stamens make pollen more accessible to animal pollinators or wind.

• The carpel houses the female reproductive structures with the elongated style being more accessible to pollinators or pollen carried by the wind

Flowers adapted for reproduction

Preparing Students for the Future xvii

Each Connecting the Concept unit (a Core concept, secondary concept, and bulleted list) is picked

up from the end-of-chapter features This reinforces the overarching hierarchy of the Core concepts, tying together seemingly unrelated

same Core concepts are found throughout the book, establishing the conceptual framework into which they can insert new knowledge

Trang 19

Detailed Feedback in Connect®

Learning is a process of iterative development, of making

mistakes, reflecting, and adjusting over time The question and

test banks in Connect® for Biology, 12th edition, are more than

direct assessments; they are self-contained learning

experi-ences that systematically build student learning over time

For many students, choosing the right answer is not

necessarily based on applying content correctly; it is more a

matter of increasing their statistical odds of guessing A major

fault with this approach is students don’t learn how to process

the questions correctly, mostly because they are repeating and

reinforcing their mistakes rather than reflecting and learning

from them To help students develop problem-solving skills, all

higher level Blooms questions in Connect are supported with

hints, to help students focus on important information for

answering the questions, and detailed feedback that walks

students through the problem-solving process, using Socratic

questions in a decision-tree-style framework to scaffold

learning, where each step models and reinforces the learning process

The feedback for each higher level Blooms question (Apply, Analyze, Evaluate) follows a similar process: Clarify Question, Gather Content, Choose Answer, Reflect on Process

Unpacking the Concepts

We’ve taken problem solving a step further In each chapter, three to five higher level Blooms questions in the question and test banks are broken out by the steps of the detailed feedback Rather than leaving it up to the student to work through the detailed feedback, a second version of the ques-tion is presented in a stepwise format Following the problem-solving steps, students need to answer questions about earlier steps, such as “What is the key concept addressed by the question?” before proceeding to answer the question A professor can choose which version of the question to include

in the assignment based on the problem-solving skills of the students

Strengthen Problem-Solving Skills with Connect ®

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Graphing Interactives

To help students develop analytical skills, Connect® for Biology,

12th edition, is enhanced with interactive graphing questions

Students are presented with a scientific problem and the

opportunity to manipulate variables, producing different results

on a graph A series of questions follows the graphing activity

to assess if the student understands and is able to interpret the data and results

Quantitative Question Bank

Many chapters also contain a Quantitative Question Bank

These are more challenging algorithmic questions, intended to

help your students practice their quantitative reasoning skills

Hints and guided solution options step students through a

problem

Trang 21

You’re in the driver’s seat.

Want to build your own course? No problem Prefer to use our turnkey,

prebuilt course? Easy Want to make changes throughout the semester?

Sure And you’ll save time with Connect’s auto-grading too

They’ll thank you for it.

Adaptive study resources like SmartBook® help your students be better prepared in less time You can transform your class time from dull definitions to dynamic debates Hear from your peers about the benefits of Connect at www.mheducation.com/highered/connect

Make it simple, make it affordable.

Connect makes it easy with seamless integration using any of the

major Learning Management Systems—Blackboard®, Canvas,

and D2L, among others—to let you organize your course in one

convenient location Give your students access to digital materials

at a discount with our inclusive access program Ask your

McGraw-Hill representative for more information

Solutions for your challenges.

A product isn’t a solution Real solutions are affordable, reliable, and come with training and ongoing support when you need it and how you want it Our Customer Experience Group can also help you troubleshoot tech problems—although Connect’s 99% uptime means you might not need to call them See for yourself at status.mheducation.com

Students—study more efficiently, retain more and achieve better outcomes Instructors—focus

on what you love—teaching.

SUCCESSFUL SEMESTERS INCLUDE CONNECT

65%

Less Time Grading

©Hill Street Studios/Tobin Rogers/Blend Images LLC

For Instructors

Trang 22

Effective, efficient studying.

Connect helps you be more productive with your

study time and get better grades using tools like

SmartBook, which highlights key concepts and creates

a personalized study plan Connect sets you up for

success, so you walk into class with confidence and

walk out with better grades

Study anytime, anywhere.

Download the free ReadAnywhere app and access your online eBook when it’s convenient, even if you’re offline

And since the app automatically syncs with your eBook in Connect, all of your notes are available every time you open

it Find out more at www.mheducation.com/readanywhere

No surprises

The Connect Calendar and Reports tools

keep you on track with the work you need

to get done and your assignment scores

Life gets busy; Connect tools help you

keep learning through it all

Learning for everyone

McGraw-Hill works directly with Accessibility Services Departments and faculty to meet the learning needs of all students Please contact your Accessibility Services office and ask them to email accessibility@mheducation.com, or visit www.mheducation.com/about/accessibility.html for

more information

made it easy to study when

you don't have your

Eastern Washington University

Chapter 7 Quiz Chapter 13 Evidence of Evolution Chapter 11 DNA Technology

Chapter 7 DNA Structure and Gene

and 7 more

©Shutterstock/wavebreakmedia

For Students

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Part I The Molecular Basis of Life

Introduction

You are about to embark on a journey—a journey of discovery about the nature of life More than 180 years ago, a young English

naturalist named Charles Darwin set sail on a similar journey on board H.M.S Beagle; a replica of this ship is pictured here What

Darwin learned on his five-year voyage led directly to his development of the theory of evolution by natural selection, a theory that has

become the core of the science of biology Darwin’s voyage seems a fitting place to begin our exploration of biology—the scientific

study of living organisms and how they have evolved Before we begin, however, let’s take a moment to think about what biology is

and why it’s important.

This is the most exciting time to be studying biology in the history

of the field The amount of information available about the natural world has exploded in the last 42 years, since the construction of the first recombinant DNA molecule We are now in a position to ask and answer questions that previously were only dreamed of.The 21st century began with the completion of the sequence

of the human genome The largest single project in the history of biology took about 20 years Yet less than 15 years later, we can sequence an entire genome in a matter of days This flood of se-quence data and genomic analysis are altering the landscape of biology These and other discoveries are also moving into the

Chapter Contents

1.1 The Science of Life

1.2 The Nature of Science

1.3 An Example of Scientific Inquiry:

Darwin and Evolution

1.4 Core Concepts in Biology

The Science of Biology

Learning Outcomes

1 Compare biology to other natural sciences.

2 Describe the characteristics of living systems.

3 Characterize the hierarchical organization of living systems.

©Soames Summerhays/Natural Visions

Trang 25

Organ system Organism Population Species Community Ecosystem

clinic as never before, with new tools for diagnostics and

treat-ment With robotics, next-generation DNA sequencing

technolo-gies, advanced imaging, and analytical techniques, we have tools

available that were formerly the stuff of science fiction

In this text, we attempt to draw a contemporary picture of the

science of biology, as well as provide some history and

experimen-tal perspective on this exciting time in the discipline In this

intro-ductory chapter, we examine the nature of biology and the

foundations of science in general to put into context the

informa-tion presented in the rest of the text

Biology unifies much of natural science

The study of biology is a point of convergence for the information

and tools from all of the natural sciences Biological systems are

the most complex chemical systems on Earth, and their many

func-tions are both determined and constrained by the principles of

chemistry and physics Put another way, no new laws of nature can

be gleaned from the study of biology—but that study does

illumi-nate and illustrate the workings of those natural laws

The intricate chemical workings of cells can be understood

using the tools and principles of chemistry And every level of

bio-logical organization is governed by the nature of energy

transac-tions first studied by thermodynamics Biological systems do not

represent any new forms of matter, and yet they are the most

com-plex organization of matter known The comcom-plexity of living

sys-tems is made possible by a constant source of energy—the Sun

The conversion of this radiant energy into organic molecules by

photosynthesis is one of the most beautiful and complex reactions

known in chemistry and physics

The way we do science is changing to grapple with ingly difficult modern problems Science is becoming more interdis-ciplinary, combining the expertise from a variety of traditional disciplines and emerging fields such as nanotechnology Biology is at the heart of this multidisciplinary approach because biological prob-lems often require many different approaches to arrive at solutions

increas-Life defies simple definition

In its broadest sense, biology is the study of living things—the

science of life. Living things come in an astounding variety of shapes and forms, and biologists study life in many different ways

They live with gorillas, collect fossils, and listen to whales They read the messages encoded in the long molecules of heredity and count how many times a hummingbird’s wings beat each second

What makes something “alive”? Anyone could deduce that a galloping horse is alive and a car is not, but why? We cannot say,

“If it moves, it’s alive,” because a car can move, and gelatin can wiggle in a bowl They certainly are not alive Although we cannot define life with a single simple sentence, we can come up with a series of seven characteristics shared by living systems:

Cellular organization All organisms consist of one or

more cells Often too tiny to see, cells carry out the basic activities of living Each cell is bounded by a membrane that separates it from its surroundings

Ordered complexity All living things are both complex and

highly ordered Your body is composed of many different kinds of cells, each containing many complex molecular structures Many nonliving things may also be complex, but they do not exhibit this degree of ordered complexity

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Organ system Organism Population Species Community Ecosystem

Sensitivity All organisms respond to stimuli Plants grow

toward a source of light, and the pupils of your eyes dilate when you walk into a dark room

Growth, development, and reproduction All organisms

are capable of growing and reproducing, and they all possess hereditary molecules that are passed to their offspring, ensuring that the offspring are of the same species

Energy utilization All organisms take in energy and use it

to perform many kinds of work Every muscle in your body

is powered with energy you obtain from your diet

Homeostasis All organisms maintain relatively constant internal

conditions that are different from their environment, a process

called homeostasis For example, your body temperature

remains stable despite changes in outside temperatures

Evolutionary adaptation All organisms interact with other

organisms and the nonliving environment in ways that influence their survival, and as a consequence, organisms evolve adaptations to their environments

Living systems show hierarchical organization

The organization of the biological world is hierarchical—that is, each level builds on the level below it:

1 The cellular level At the cellular level (figure 1.1), atoms, the fundamental elements of matter, are joined together into clusters called molecules Complex

biological molecules are assembled into tiny structures

called organelles within membrane-bounded units

we call cells The cell is the basic unit of life Many

independent organisms are composed only of single

cells Bacteria are single cells, for example All animals and plants, as well as most fungi and algae, are multicellular—composed of more than one cell

2 The organismal level Cells in complex multicellular

organisms exhibit three levels of organization The most

basic level is that of tissues, which are groups of similar

cells that act as a functional unit Tissues, in turn, are

grouped into organs—body structures composed of

several different tissues that act as a structural and functional unit Your brain is an organ composed of nerve cells and a variety of associated tissues that form protective coverings and contribute blood At the third

level of organization, organs are grouped into organ systems The nervous system, for example, consists of

sensory organs, the brain and spinal cord, and neurons that convey signals

Figure 1.1 Hierarchical organization of living systems. Life forms a hierarchy of organization from atoms to complex multicellular organisms Atoms are joined together to form molecules, which are assembled into more complex structures such as organelles These in turn form subsystems that provide different functions Cells can be organized into tissues, then into organs and organ systems such

as the goose’s nervous system pictured This organization then extends beyond individual organisms to populations, communities, ecosystems, and finally the biosphere (Organelle): ©Keith R Porter/Science Source; (Cell):

©STEVE GSCHMEISSNER/Getty Images; (Tissue): ©Ed Reschke; (Organism):

©Russell Illig/Getty Images; (Population): ©George Ostertag/age fotostock;

(Species): ©iStockphoto/Getty Images; ©Pictureguy/Shutterstock; (Community):

©Ryan McGinnis/Alamy; (Ecosystem): ©Robert and Jean Pollock; (Biosphere):

Source: NASA

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3 The populational level Individual organisms can

be categorized into several hierarchical levels within

the living world The most basic of these is the

population—a group of organisms of the same species

living in the same place All populations of a particular

kind of organism together form a species, its members

similar in appearance and able to interbreed At a higher

level of biological organization, a biological community

consists of all the populations of different species living

together in one place

4 The ecosystem level At the highest tier of biological

organization, populations of organisms interact with

each other and their physical environment Together

populations and their environment constitute an

ecological system, or ecosystem For example, the

biological community of a mountain meadow interacts

with the soil, water, and atmosphere of a mountain

ecosystem in many important ways

5 The biosphere The entire planet can be thought of as an

ecosystem that we call the biosphere

As you move up this hierarchy, the many interactions occurring at

lower levels can produce novel properties These so-called

emergent properties may not be predictable Examining

individ-ual cells, for example, gives little hint about the whole animal

Many weather phenomena, such as hurricanes, are actually

emer-gent properties of many interacting meteorological variables It is

because the living world exhibits many emergent properties that it

is difficult to define “life.”

This description of the common features and organization of

living systems provides an introduction for our exploration of

biol-ogy Before we continue, we will consider the broader question of

the nature of science itself

Learning Outcomes Review 1.1

Biology as a science brings together other natural sciences, such

as chemistry and physics, to study living systems Life does not

have a simple definition, but living systems share a number of

properties that together describe life Living systems can be

organized hierarchically, from the cellular level to the entire

biosphere, with emergent properties that may exceed the sum of

the parts.

Can you study biology without studying other sciences?

Learning Outcomes

1 Compare the different types of reasoning used by biologists.

2 Demonstrate how to formulate and test a hypothesis.

Much like life itself, the nature of science defies simple

descrip-tion For many years scientists have written about the “scientific

method” as though there is a single way of doing science

This oversimplification has contributed to confusion on the part of nonscientists about the nature of science

At its core, science is concerned with developing an ingly accurate understanding of the world around us using observa-tion and reasoning To begin with, we assume that natural forces acting now have always acted, that the fundamental nature of the uni-verse has not changed since its in ception, and that it is not changing now A number of complementary approaches allow understanding

increas-of natural phenomena—there is no one “scientific method.”

Scientists also attempt to be as objective as possible in the interpretation of the data and observations they have collected

Because scientists themselves are human, this is not completely possible, but because science is a collective endeavor subject to scrutiny, it is self-correcting One person’s results are verified by others, and if the results cannot be repeated, they are rejected

Much of science is descriptive

The classic vision of the scientific method is that observations lead

to hypotheses that in turn make experimentally testable tions In this way, we dispassionately evaluate new ideas to arrive

predic-at an increasingly accurpredic-ate view of npredic-ature We discuss this way of doing science later in this section but it is important to understand that much of science is purely descriptive: In order to understand anything, the first step is to describe it completely Much of biol-ogy is concerned with arriving at an increasingly accurate descrip-tion of nature

The study of biodiversity is an example of descriptive ence that has implications for other aspects of biology in addition

sci-to societal implications Efforts are currently under way sci-to classify all life on Earth This ambitious project is purely descriptive, but it will lead to a much greater understanding of biodiversity as well as the effect our species has on biodiversity

One of the most important accomplishments of molecular biology at the dawn of the 21st century was the completion of the sequence of the human genome Many new hypotheses about human biology will be generated by this knowledge, and many experiments will be needed to test these hypotheses, but the determination of the sequence itself was descriptive science

Science uses both deductive and inductive reasoning

The study of logic recognizes two opposite ways of arriving at logical conclusions: deductive and inductive reasoning Science makes use of both of these methods, although induction is the primary way of reasoning in hypothesis-driven science

Deductive reasoning

Deductive reasoning applies general principles to predict

spe-cific results More than 2200 years ago, the Greek scientist Eratosthenes used Euclidean geometry and deductive reasoning

to accurately estimate the circumference of the Earth ( figure 1.2)

Deductive reasoning is the reasoning of mathematics and phi- losophy, and it is used to test the validity of general ideas in all

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at midday

Well

Light rays parallel

Height of obelisk

Distance between cities = 800 km

Length of shadow a

a

Observation

Predictions

Predictions confirmed

Question

Hypothesis 1 Hypothesis 2 Hypothesis 3 Hypothesis 4 Hypothesis 5

Potential hypotheses

Remaining possible hypotheses

Last remaining possible hypothesis

Reject hypotheses

2 and 3

Reject hypotheses

1 and 4

Hypothesis 2 Hypothesis 3 Hypothesis 5

branches of knowledge For example, if all mammals by

defini-tion have hair, and you find an animal that does not have hair,

then you may conclude that this animal is not a mammal A

bi-ologist uses deductive reasoning to infer the species of a

speci-men from its characteristics

Inductive reasoning

In inductive reasoning, the logic flows in the opposite direction,

from the specific to the general Inductive reasoning uses specific

observations to construct general scientific principles For example,

if poodles have hair, and terriers have hair, and every dog that you

observe has hair, then you may conclude that all dogs have hair

In-ductive reasoning leads to generalizations that can then be tested

Inductive reasoning first became important to science in the 1600s

in Europe, when Francis Bacon, Isaac Newton, and others began to

use the results of particular experiments to infer general principles

about how the world operates

An example from modern biology is the role of homeobox

genes in development Studies in the fruit fly, Drosophila

melano-gaster, identified genes that could cause dramatic changes in

de-velopmental fate, such as a leg appearing in the place of an antenna

These genes have since been found in essentially all multicellular

animals analyzed This led to the general idea that homeobox

genes control developmental fate in animals

Hypothesis-driven science

makes and tests predictions

Scientists establish which general principles are true from among

the many that might be true through the process of systematically

testing alternative proposals If these proposals prove inconsistent

with experimental observations, they are rejected as untrue

Figure 1.3 illustrates the process

Figure 1.2 Deductive reasoning: How Eratosthenes estimated the circumference of the Earth using deductive reasoning. 1 On a

day when sunlight shone straight down a deep well at Syene in Egypt, Eratosthenes measured the length of the shadow cast by a tall obelisk in the city

of Alexandria, about 800 kilometers (km) away 2. The shadow’s length and the

obelisk’s height formed two sides of a triangle Using the recently developed

principles of Euclidean geometry, Eratosthenes calculated the angle, a, to be 7°

and 12´, exactly 1 ⁄ 50 of a circle (360°) 3 If angle a is 1 ⁄ 50 of a circle, then the distance between the obelisk (in Alexandria) and the well (in Syene) must be equal to 1 ⁄ 50 the circumference of the Earth 4 Eratosthenes had heard that

it was a 50-day camel trip from Alexandria to Syene Assuming a camel travels about 18.5 km per day, he estimated the distance between obelisk and well as 925 km (using different units of measure, of course)

5. Eratosthenes thus deduced the circumference of the Earth to be

50 × 925 = 46,250 km Modern measurements put the distance from the well to the obelisk at just over 800 km Using this distance Eratosthenes’s value would have been 50 × 800 = 40,000 km The actual circumference is 40,075 km.

Figure 1.3 How science is done. This diagram illustrates how scientific investigations proceed First, scientists make observations that raise a particular question They develop a number

of potential explanations (hypotheses) to answer the question Next, they carry out experiments in an attempt to eliminate one or more of these hypotheses Then, predictions are made based on the remaining hypotheses, and further experiments are carried out to test these predictions The process can also be iterative As experimental results are performed, the information can be used to modify the original hypothesis to fit each new observation.

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After making careful observations, scientists construct a

hypothesis, which is a suggested explanation that accounts for

those observations A hypothesis is a proposition that might be

true Those hypotheses that have not yet been disproved are

re-tained They are useful because they fit the known facts, but they

are always subject to future rejection if, in the light of new

infor-mation, they are found to be incorrect

This is usually an ongoing process with a hypothesis

chang-ing and bechang-ing refined with new data For instance, geneticists

George Beadle and Edward Tatum studied the nature of genetic

information to arrive at their “one-gene/one-enzyme” hypothesis

(see chapter 15) This hypothesis states that a gene represents the

genetic information necessary to make a single enzyme As

inves-tigators learned more about the molecular nature of genetic

infor-mation, the hypothesis was refined to “one gene/one polypeptide”

because enzymes can be made up of more than one polypeptide

With still more information about the nature of genetic

informa-tion, other investigators found that a single gene can specify more

than one polypeptide, and the hypothesis was refined again

Testing hypotheses

We call the test of a hypothesis an experiment Suppose you enter

a dark room To understand why it is dark, you propose several

hypotheses The first might be, “There is no light in the room

be-cause the light switch is turned off.” An alternative hypothesis

might be, “There is no light in the room because the lightbulb is

burned out.” And yet another hypothesis might be, “I am going

blind.” To evaluate these hypotheses, you would conduct an

ex-periment designed to eliminate one or more of the hypotheses

For example, you might test your hypotheses by flipping the

light switch If you do so and the room is still dark, you have

dis-proved the first hypothesis: Something other than the setting of the

light switch must be the reason for the darkness Note that a test

such as this does not prove that any of the other hypotheses are

true; it merely demonstrates that the one being tested is not A

suc-cessful experiment is one in which one or more of the alternative

hypotheses is demonstrated to be inconsistent with the results and

is thus rejected

As you proceed through this text, you will encounter many

hypotheses that have withstood the test of experiment Many will

continue to do so; others will be revised as new observations are

made by biologists Biology, like all science, is in a constant state of

change, with new ideas appearing and replacing or refining old ones

Establishing controls

Often scientists are interested in learning about processes that are

influenced by many factors, or variables To evaluate alternative

hy-potheses about one variable, all other variables must be kept constant

This is done by carrying out two experiments in parallel: an

experi-mental treatment or group and a control treatment or group In

the experimental treatment, one variable is altered in a known way to

test a particular hypothesis In the control treatment, that variable is

left unaltered In all other respects the two experiments are identical,

so any difference in the outcomes of the two experiments must result

from the influence of the variable that was changed

Much of the challenge of experimental science lies in

de-signing control experiments that isolate a particular variable from

other factors that might influence a process

Using predictions

A successful scientific hypothesis needs to be not only valid but also useful—it needs to tell us something we want to know A hy-pothesis is most useful when it makes predictions because those predictions provide a way to test the validity of the hypothesis If

an experiment produces results inconsistent with the predictions, the hypothesis must be rejected or modified In contrast, if the pre-dictions are supported by experimental testing, the hypothesis is supported The more experimentally supported predictions a hy-pothesis makes, the more valid the hypothesis is

As an example, in the early history of microbiology it was known that nutrient broth left sitting exposed to air becomes con-taminated Two hypotheses were proposed to explain this observa-tion: spontaneous generation and the germ hypothesis Spontaneous generation held that there was an inherent property in organic mol-ecules that could lead to the spontaneous generation of life The germ hypothesis proposed that preexisting microorganisms that were present in the air could contaminate the nutrient broth

These competing hypotheses were tested by a number of periments that involved filtering air and boiling the broth to kill any contaminating germs The definitive experiment was per-formed by Louis Pasteur, who constructed flasks with curved necks that could be exposed to air, but that would trap any con-taminating germs When such flasks were boiled to sterilize them, they remained sterile, but if the curved neck was broken off, they became contaminated (figure 1.4)

ex-Result: No growth occurs in sterile swan-necked flasks When the

neck is broken off, and the broth is exposed to air, growth occurs.

Conclusion: Growth in broth is of preexisting microorganisms.

SCIENTIFIC THINKING

Question: What is the source of contamination that occurs in a flask

of nutrient broth left exposed to the air?

Germ Hypothesis: Preexisting microorganisms present in the air

contaminate nutrient broth.

Prediction: Sterilized broth will remain sterile if microorganisms are

prevented from entering flask.

Spontaneous Generation Hypothesis: Living organisms will

spontaneously generate from nonliving organic molecules in broth.

Prediction: Organisms will spontaneously generate from organic

molecules in broth after sterilization.

Test: Use swan-necked flasks to prevent entry of microorganisms To

ensure that broth can still support life, break swan-neck after sterilization.

Flask is sterilized

by boiling the broth. Unbroken flaskremains sterile. Broken flask becomescontaminated after

exposure to germ-laden air.

Broken neck

of flask

Figure 1.4 Experiment to test spontaneous generation versus germ hypothesis.

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This result was predicted by the germ hypothesis—that when the sterile flask is exposed to air, airborne germs are depos-

ited in the broth and grow The spontaneous generation

hypothe-sis predicted no difference in results with exposure to air This

experiment disproved the hypothesis of spontaneous generation

and supported the hypothesis of airborne germs under the

condi-tions tested

Reductionism breaks larger systems

into their component parts

Scientists use the philosophical approach of reductionism to

un-derstand a complex system by reducing it to its working parts

Reductionism has been the general approach of biochemistry,

which has been enormously successful at unraveling the complexity

of cellular metabolism by concentrating on individual pathways

and specific enzymes By analyzing all of the pathways and their

components, scientists now have an overall picture of the

metabo-lism of cells

Reductionism has limits when applied to living systems, however—one of which is that enzymes do not always behave

exactly the same in isolation as they do in their normal cellular

context A larger problem is that the complex interworking of

many interconnected functions leads to emergent properties that

cannot be predicted based on the workings of the parts For

ex-ample, ribosomes are the cellular factories that synthesize

pro-teins, but this function could not be predicted based on analysis

of the individual proteins and RNA that make up the structure

On a higher level, understanding the physiology of a single

Canada goose would not lead to predictions about flocking

be-havior The emerging field of systems biology uses mathematical

and computational models to deal with the whole as well as

understanding the interacting parts

Biologists construct models

to explain living systems

Biologists construct models in many different ways for a variety of

uses Geneticists construct models of interacting networks of

proteins that control gene expression, often even drawing cartoon

figures to represent that which we cannot see Population biologists

build models of how evolutionary change occurs Cell biologists

build models of signal transduction pathways and the events

leading from an external signal to internal events Structural

biolo-gists build actual models of the structure of proteins and

macromo-lecular complexes in cells

Models provide a way to organize how we think about a problem Models can also get us closer to the larger picture and

away from the extreme reductionist approach The working parts

are provided by the reductionist analysis, but the model shows how

they fit together Often these models suggest other experiments

that can be performed to refine or test the model

As researchers gain more knowledge about the actual flow of molecules in living systems, more sophisticated kinetic models

can be used to apply information about isolated enzymes to their

cellular context In systems biology, this modeling is being applied

on a large scale to regulatory networks during development, and

even to modeling an entire bacterial cell

The nature of scientific theories

Scientists use the word theory in two main ways The first meaning

of theory is a proposed explanation for some natural phenomenon, often based on some general principle Thus, we speak of the prin-ciple first proposed by Newton as the “theory of gravity.” Such theories often bring together concepts that were previously thought

to be unrelated

The second meaning of theory is the body of interconnected concepts, supported by scientific reasoning and experimental evi-dence, that explains the facts in some area of study Such a theory provides an indispensable framework for organizing a body of knowledge For example, quantum theory in physics brings together

a set of ideas about the nature of the universe, explains experimental facts, and serves as a guide to further questions and experiments

To a scientist, theories are the solid ground of science, pressing ideas of which we are most certain In contrast, to the gen-

ex-eral public, the word theory usually implies the opposite—a lack of

knowledge, or a guess Not surprisingly, this difference often sults in confusion In this text, theory will always be used in its scientific sense, in reference to an accepted general principle or body of knowledge

re-Some critics outside of science attempt to discredit evolution

by saying it is “just a theory.” The hypothesis that evolution has occurred, however, is an accepted scientific fact—it is supported

by overwhelming evidence Modern evolutionary theory is a plex body of ideas, the importance of which spreads far beyond explaining evolution Its ramifications permeate all areas of biol-ogy, and it provides the conceptual framework that unifies biology

com-as a science Again, the key is how well a hypothesis fits the vations Evolutionary theory fits the observations very well

obser-Research can be basic or applied

In the past it was fashionable to speak of the “scientific method” as consisting of an orderly sequence of logical, either–or steps Each step would reject one of two mutually incompatible alternatives, as though trial-and-error testing would inevitably lead a researcher through the maze of uncertainty to the ultimate scientific answer

If this were the case, a computer would make a good scientist But science is not done this way

As the British philosopher Karl Popper has pointed out, cessful scientists without exception design their experiments with a pretty fair idea of how the results are going to come out They have what Popper calls an “imaginative preconception” of what the truth might be Because insight and imagination play such a large role in scientific progress, some scientists are better at science than others—just as Bruce Springsteen stands out among songwriters or Claude Monet stands out among Impressionist painters

suc-Some scientists perform basic research, which is intended to

extend the boundaries of what we know These individuals cally work at universities, and their research is usually supported

typi-by grants from various agencies and foundations

The information generated by basic research contributes to the growing body of scientific knowledge, and it provides the scien-

tific foundation utilized by applied research Scientists who

con-duct applied research are often employed in some kind of industry Their work may involve the manufacture of food additives, the cre-ation of new drugs, or the testing of environmental quality

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Research results are published in scientific journals, where

the experiments and conclusions are reviewed by other scientists

This process of careful evaluation, called peer review, lies at the

heart of modern science It helps to ensure that faulty research or

false claims are challenged and not accepted without examination

Recently, there have been problems raised with reproducibility in

some areas of biology That this is being examined indicates the

self-reflective nature of science

With some idea of what science is and how it functions, we

will consider a single example in detail What better example than

the development of one of the most important ideas in the history

of science: Darwin’s theory of evolution by natural selection

Learning Outcomes Review 1.2

Much of science is descriptive, amassing observations to gain an

accurate view Both deductive reasoning and inductive reasoning

are used in science Scientific hypotheses are suggested

explanations for observed phenomena Hypotheses need to

make predictions that can be tested by controlled experiments

Theories are coherent explanations of observed data, but they

may be modified by new information.

How does a scientific theory differ from a hypothesis?

2 Describe the evidence that supports the theory of evolution.

Darwin’s theory of evolution explains and describes how

organ-isms on Earth have changed over time and acquired a diversity of

new forms This famous theory provides a good example of how a

scientist develops a hypothesis and how a scientific theory grows

and wins acceptance

Charles Robert Darwin (1809–1882; figure 1.5) was an

English naturalist who, after 30 years of study and observation,

wrote one of the most famous and influential books of all time

This book, On the Origin of Species by Means of Natural Selection,

created a sensation when it was published, and the ideas Darwin

expressed in it have played a central role in the development of

human thought ever since

The idea of evolution existed prior to Darwin

In Darwin’s time, most people believed that the different kinds of

organisms and their individual structures resulted from direct

ac-tions of a Creator (many people still believe this) Species were

thought to have been specially created and to be unchangeable over the course of time

In contrast to these ideas, a number of earlier naturalists and philosophers had presented the view that living things must have

changed during the history of life on Earth That is, evolution has

occurred, and living things are now different from how they began

Darwin’s contribution was a concept he called natural selection,

which he proposed as a coherent, logical explanation for this process, and he brought his ideas to wide public attention

Darwin observed differences

in related organisms

The story of Darwin and his theory begins in 1831, when he was

22 years old He was part of a five-year navigational mapping expedition around the coasts of South America ( figure  1.6),

aboard H.M.S Beagle During this long voyage, Darwin had the

chance to study a wide variety of plants and animals on continents and islands and in distant seas Darwin observed a number of phenomena that were of central importance to his reaching his ultimate conclusion

Repeatedly, Darwin saw that the characteristics of similar species varied somewhat from place to place These geographical patterns suggested to him that lineages change gradually as species migrate from one area to another On the Galápagos Islands,

960 km (600 miles) off the coast of Ecuador, Darwin encountered

a variety of different finches on the various islands The 14 species, although related, differed slightly in appearance, particularly in their beaks (figure 1.7)

Darwin thought it was reasonable to assume that all these birds had descended from a common ancestor arriving from the South American mainland several million years ago Eating differ-ent foods on different islands, the finches’ beaks had changed during their descent—“descent with modification,” or evolution (These finches are discussed in more detail in chapters 21 and 22.)

Figure 1.5 Charles Darwin. This newly rediscovered photograph taken in 1881, the year before Darwin died, appears to be the last ever taken of the great biologist ©Huntington Library/SuperStock

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British Isles Western Isles

E U R O P E

A F R I C A

Madagascar Mauritius Bourbon Island

Friendly Islands

Philippine Islands

Marquesas

Galápagos Islands

Valparaiso Society

Islands Straits of Magellan

Tierra del Fuego Cape Horn

Falkland Islands

N O R T H

A M E R I C A

Canary Islands

Keeling Islands

Woodpecker Finch (Cactospiza pallida) Large Ground Finch (Geospiza magnirostris) Cactus Finch (Geospiza scandens)

In a more general sense, Darwin was struck by the fact that the plants and animals on these relatively young volcanic islands

resembled those on the nearby coast of South America If each one

of these plants and animals had been created independently and

simply placed on the Galápagos Islands, why didn’t they resemble

the plants and animals of islands with similar climates—such as

those off the coast of Africa, for example? Why did they resemble

those of the adjacent South American coast instead?

Darwin proposed natural selection

as a mechanism for evolution

It is one thing to observe the results of evolution, but quite another

to understand how it happens Darwin’s great achievement lies in his ability to move beyond all the individual observations to for-mulate the hypothesis that evolution occurs because of natural selection

Figure 1.6 The five-year voyage of H.M.S Beagle. Most of the time was spent exploring the coasts and coastal islands of South

America, such as the Galápagos Islands Darwin’s studies of the animals of the Galápagos Islands played a key role in his eventual development

of the concept of evolution by means of natural selection.

Figure 1.7 Three Galápagos finches and what they eat. On the Galápagos Islands, Darwin observed 14 different species of finches differing mainly in their beaks and feeding habits These three finches eat very different food items, and Darwin surmised that the different

shapes of their bills represented evolutionary adaptations that improved their ability to eat the foods available in their specific habitats.

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Darwin and Malthus

Of key importance to the development of Darwin’s insight was his

study of Thomas Malthus’s An Essay on the Principle of Population

(1798) In this book, Malthus stated that populations of plants and

animals (including humans) tend to increase geometrically, while

humans are able to increase their food supply only arithmetically

Put another way, population increases by a multiplying factor—for

example, in the series 2, 6, 18, 54, the starting number is multiplied

by 3 Food supply increases by an additive factor—for example, the

series 2, 4, 6, 8 adds 2 to each starting number Figure 1.8 shows the

difference that these two types of relationships produce over time

Because populations increase geometrically, virtually any

kind of animal or plant, if it could reproduce unchecked, would

cover the entire surface of the world surprisingly quickly Instead,

populations of species remain fairly constant year after year,

be-cause death limits population numbers

Sparked by Malthus’s ideas, Darwin saw that although every

organism has the potential to produce more offspring than can

sur-vive, only a limited number actually do survive and produce further

offspring Combining this observation with what he had seen on the

voyage of the Beagle, as well as with his own experiences in breeding

domestic animals, Darwin made an important association: als possessing physical, behavioral, or other attributes that give them

Individu-an advIndividu-antage in their environment are more likely to survive Individu-and produce than those with less advantageous traits By surviving, these individuals gain the opportunity to pass on their favorable character-istics to their offspring As the frequency of these characteristics in-creases in the population, the nature of the population as a whole will

re-gradually change Darwin called this process selection.

Natural selection

Darwin was thoroughly familiar with variation in domesticated

animals, and he began On the Origin of Species with a detailed

discussion of pigeon breeding He knew that animal breeders selected certain varieties of pigeons and other animals, such as dogs, to produce certain characteristics, a process Darwin called

artificial selection.

Artificial selection often produces a great variation in traits

Domestic pigeon breeds, for example, show much greater variety than all of the wild species found throughout the world Darwin thought that this type of change could occur in nature, too Surely if pigeon breeders could foster variation by artificial selection, nature

could do the same—a process Darwin called natural selection.

Darwin drafts his argument

Darwin drafted the overall argument for evolution by natural tion in a preliminary manuscript in 1842 After showing the manu-script to a few of his closest scientific friends, however, Darwin put it in a drawer, and for 16 years turned to other research No one knows for sure why Darwin did not publish his initial manuscript—

selec-it is very thorough and outlines his ideas in detail

The stimulus that finally brought Darwin’s hypothesis into print was an essay he received in 1858 A young English naturalist named Alfred Russel Wallace (1823–1913) sent the essay to Dar-win from Indonesia; it concisely set forth the hypothesis of evolu-tion by means of natural selection, a hypothesis Wallace had developed independently of Darwin After receiving Wallace’s es-say, friends of Darwin arranged for a joint presentation of their ideas at a seminar in London Darwin then completed his own book, expanding the 1842 manuscript he had written so long ago, and submitted it for publication

The predictions of natural selection have been tested

More than 130 years have elapsed since Darwin’s death in 1882

During this period, the evidence supporting his theory has grown progressively stronger We briefly explore some of this evidence here; in chapter 21, we will return to the theory of evolution by natural selection and examine the evidence in more detail

The fossil record

Darwin predicted that the fossil record would yield intermediate links between the great groups of organisms—for example, be-tween fishes and the amphibians thought to have arisen from them, and between reptiles and birds Furthermore, natural selection pre-dicts the relative positions in time of such transitional forms We now know the fossil record to a degree that was unthinkable in the

Figure 1.8 Geometric and arithmetic progressions. A

geometric progression increases by a constant factor (for example, the

curve shown increases ×3 for each step), whereas an arithmetic

progression increases by a constant difference (for example, the line

shown increases +2 for each step) Malthus contended that the human

growth curve was geometric, but the human food production curve

was only arithmetic.

constant factor for a geometric progression? How would this

change the curve in the figure?

Inquiry question Might this effect be achieved with

humans? How?

?

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Human Cat Bat Porpoise Horse

Number of Amino Acid Differences in a Hemoglobin Polypeptide

?

19th century, and although truly “intermediate” organisms are

hard to determine, paleontologists have found what appear to be

transitional forms and found them at the predicted positions

in time

Analysis of microscopic fossils extends the history of life on Earth to about 3.5 billion years ago (bya) The discovery of other

fossils has supported Darwin’s predictions and has shed light on

how organisms have, over this enormous time span, evolved from

the simple to the complex For vertebrate animals especially, the

fossil record is rich and exhibits a graded series of changes in form,

with the evolutionary sequence visible for all to see

The age of the Earth

Darwin’s theory predicted the Earth must be very old, but some

physicists argued that the Earth was only 100 million years old

This bothered Darwin, because this did not seem to allow enough

time for the evolution of all living things from a common ancestor

Using evidence obtained by studying the rates of radioactive

de-cay, we now know that the physicists of Darwin’s time were very

wrong: The Earth was formed about 4.5 bya

The mechanism of heredity

Darwin received some of his sharpest criticism in the area of

he-redity At that time, no one had any concept of genes or how

hered-ity works, so it was not possible for Darwin to explain completely

how evolution occurs

Even though Gregor Mendel was performing his ments with pea plants in Brünn, Austria (now Brno, the Czech

experi-Republic), during roughly the same period, genetics was

estab-lished as a science only at the start of the 20th century When

sci-entists began to understand the laws of inheritance (discussed in

chapters 12 and 13), this problem with Darwin’s theory vanished

Comparative anatomy

Comparative studies of animals have provided strong evidence for

Darwin’s theory In many different types of vertebrates, for

exam-ple, the same bones are present, indicating their evolutionary past

Thus, the forelimbs shown in figure 1.9 are all constructed from

the same basic array of bones, modified for different purposes

These bones are said to be homologous in the different

vertebrates—that is, they have the same evolutionary origin, but

they now differ in structure and function They are contrasted with

analogous structures, such as the wings of birds and butterflies,

which have similar function but different evolutionary origins

Molecular evidence

Evolutionary patterns are also revealed at the molecular level By comparing the genomes (that is, the sequences of all the genes) of different groups of animals or plants, we can more precisely spec-ify the degree of relationship among the groups A series of evolu-tionary changes over time should involve a continual accumulation

of genetic changes in the DNA

This difference can be seen clearly in the protein bin (figure 1.10) Rhesus monkeys, which like humans are pri-mates, have fewer differences from humans in the 146-amino-acid

hemoglo-Figure 1.9 Homology among vertebrate limbs.

The forelimbs of these five vertebrates show the ways in which the relative proportions of the forelimb bones have changed

in relation to the particular way of life of each organism.

Figure 1.10 Molecules reflect evolutionary patterns.

Vertebrates that are more distantly related to humans have a greater number of amino acid differences in the hemoglobin polypeptide.

Inquiry question Where do you imagine a snake might fall on the graph? Why?

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hemoglobin β chain than do more distantly related mammals, such

as dogs Nonmammalian vertebrates, such as birds and frogs,

dif-fer even more This kind of analysis allows us to construct

phylo-genetic trees that provide a graphic representation of these

evolutionary relationships

We will explore these ideas in much more detail in Part IV

For now we will conclude our introduction to biology by

consider-ing how we can use core concepts to organize our thinkconsider-ing and deal

with the enormous amount of information that is modern biology

Learning Outcomes Review 1.3

Darwin observed differences in related organisms and proposed

the hypothesis of evolution by natural selection to explain these

differences The predictions generated by natural selection have

been tested and continue to be tested by analysis of the fossil

record, genetics, comparative anatomy, and even the DNA of

living organisms.

Does Darwin’s theory of evolution by natural selection

explain the origin of life?

Learning Outcome

1 Discuss the core concepts that underlie the study of biology.

At the fundamental level of neurochemistry, the brain of a novice

is largely the same as that of an expert There are however,

signifi-cant differences in how experts organize the information they

col-lect over time As you are starting to gather information about

biology, it is worth considering how you can begin to organize this

information in your mind like an expert thinker

You may be trying to organize the flood of information

about biology by topics The problem with this approach is that

there are just too many topics for this to be successful A better way

to organize ideas in your mind is using a conceptual framework

Most disciplines, including biology, are based on information that

is readily organized around concepts You can think of concepts as

a place in your mind to hold specific ideas that relate to many

top-ics For example, consider a hammer, a sunflower, and DNA

These seem quite disparate, but can actually be organized

concep-tually A hammer has a long handle to create leverage and a heavy

head to drive nails Sunflowers have broad leaves that maximize

their ability to absorb light for photosynthesis, and DNA has a

structure that allows storage of information These descriptions

can be organized into the concept “structure determines

function”: the function of something arises from its form When

you encounter new information, you can fit it into a framework of

core concepts like “structure determines function.”

There has been a recent movement to emphasize core

con-cepts in biology education The authors applaud this and have

in-corporated this approach in this text We have emphasized five

core concepts: life is subject to chemical and physical laws; ture determines function; living systems transform energy and matter; living systems depend on information transactions; and evolution explains the unity and diversity of life

struc-Core concepts are, by their very nature, high level and thus general These are used to organize more specific secondary con-cepts, which in turn arise from observations, experiments, or de-scriptions of biological phenomenon For example, the core concept

“structure determines function” could lead to the secondary concept

“Genetic information is encoded in the structure of DNA.” This can then be used to organize a series of observations about the nature of genetic information and how it is used, such as these: “base pairing involves specific patterns of hydrogen bonds,” and “the genetic code consists of four nucleotides that are abbreviated: A,T,G,C,” and

“DNA is used as a template to synthesize RNA,” and so on

To keep you focused on the core concepts, and how they late to the material of each chapter, we present a Connecting the Concepts feature at the end of each chapter In each of these, we present one example of how the authors organize different ideas under a core concept A second core concept is provided for you to practice organizing ideas into your own conceptual framework

re-Then at the end of each of the eight Parts of the book is a larger Connecting the Concepts feature that does the same thing, but with

a much larger scope Due to space limitations this will not sarily include material from every chapter or section, and is in-tended as an example and not an exhaustive list

neces-The five core concepts

Life is subject to chemical and physical laws

It may seem obvious, but it is important to emphasize that living systems operate according to known chemical and physical prin-ciples For this reason, almost all introductory textbooks, including this one, begin with several sections on chemistry This is because biological systems are the ultimate application of some very com-plex chemistry However, there are no new chemical or physical laws to be found in biology, just the consistent application of famil-iar chemical principles and laws This means that some knowledge

of atomic structure, chemical bonding, thermodynamics, kinetics, and many other topics from basic chemistry and physics is crucial for understanding biological systems

It may seem that some physics and chemistry would only be relevant in the “cell and molecular” sections of the book, but in fact, those principles are applied throughout the book The move-ment of water in plants depends on the basic chemistry of water, the kidney is an osmotic machine, energy flow and nutrient cycling

in ecosystems are driven by thermodynamic laws, and the cycling

of many important elements involves biogeochemical cycles

Structure determines function

A major unifying theme of biology is the relationship between structure and function Said simply, the proper functioning of mol-ecules, of cells, and of tissues and organs depends on their struc-ture Although this observation may seem trivial, it has far-reaching implications When we know the function of a particular structure,

we can infer the function of similar structures found in different contexts, such as in different organisms

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For example, suppose we know the structure of a human cell’s surface receptor for insulin, the hormone that controls the

uptake of glucose We then find a similar molecule in the

mem-brane of a cell from a very different species, such as a worm We

might conclude that this membrane molecule acts as a receptor

for an insulin-like molecule produced by the worm In this way,

we might be able to hypothesize an evolutionary relationship

between glucose uptake in worms and in humans When structure

is altered, function is disrupted, with potential physiological

consequences

Living systems transform energy and matter

From single cells to the highest level of biological organization,

the biosphere, living systems have a constant need for energy If we

trace this all the way back, the original energy source for the

bio-sphere is the sun Without this energy, living systems would not

exhibit their characteristic highly organized state While this

sounds simple, it means that the basic nature of life is to constantly

transform both energy and matter We break down “food”

mole-cules for energy, then use this energy to build up other complex

molecules

The energy from the sun is trapped by photosynthetic isms, which use this energy to reduce CO2 and produce organic

organ-compounds The rest of us, who need a constant source of energy

and carbon, can oxidize these organic compounds back to CO2,

releasing energy to drive the processes of life As all of these

en-ergy transactions are inefficient, a certain amount of enen-ergy is also

randomized as heat

This constant input of energy allows living systems to tion far from thermodynamic equilibrium At equilibrium, you are

func-a pool of func-amino func-acids, nucleotides, func-and other smfunc-all molecules, func-and

not the complex dynamic system reading this sentence

Nonequi-librium systems also can exhibit the property of self-organization

not seen in equilibrium systems Macromolecular complexes, such

as the spindle necessary for chromosome separation, can self-

organize (figure 1.11) A flock of birds, a school of fish, and the

bacteria in a biofilm all also display self-organization, exhibiting

properties not seen in the individual parts alone

Living systems depend on information

transactions

The most obvious form of information in living systems is the

genetic information carried in every cell in the form of

deoxyribonucleic acid (DNA) Each DNA molecule is formed

from two long chains of building blocks, called nucleotides, wound

around each other (figure 1.12) Four different nucleotides are found

in DNA, and the sequence in which they occur encodes the

informa-tion to make and maintain a cell

The continuity of life from one generation to the next—

heredity—depends on the faithful copying of a cell’s DNA into

daughter cells The entire set of DNA instructions that

speci-fies a cell is called its genome The sequence of the human

genome, 3 billion nucleotides long, was decoded in rough-draft

form in 2001

However, the importance of information goes beyond genomes and their inheritance Cells are highly complex nanoma-

chines that receive, process, and respond to information The

Figure 1.11 The spindle In this dividing cell, microtubules have organized themselves into a spindle (stained red), pulling each chromosome (stained blue) to the central plane of the dividing cell

©Waheeb K Heneen/Swedish University of Agricultural Sciences

Figure 1.12 DNA, the genetic material All organisms store their hereditary information as sequences of DNA subunits, much as this textbook stores information as sequences of alphabet letters

©Science Photo Library/Alamy Stock Photo

information stored in DNA is used to direct the synthesis of lar components, and the particular set of components can differ from cell to cell The way proteins fold in space is a form of infor-mation that is three-dimensional, and interesting properties emerge from the interaction of these shapes in macromolecular complexes The control of gene expression allows the differentiation of cell

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cellu-Learning Outcome Review 1.4

Understanding biology requires higher-level concepts We are using five core concepts throughout the book: Life is subject to chemical and physical laws, structure determines function, living systems transform energy and matter, living systems depend on information transactions, and evolution explains the unity and diversity of life.

How do viruses fit into our definitions of living systems?

Mus musculus (animal) Saccharomyces cerevisiae(fungus)

Saccharomyces cerevisiae

Arabidopsis thaliana (plant)

Arabidopsis thaliana (plant)

MEIS KN BEL1 MATa1

HB8 HAT GL2 PAX6 PEM

Figure 1.13 Tree of homeodomain proteins.

Homeodomain proteins are found in fungi (brown), plants (green), and animals (blue) Based on their sequence similarities, these 11 different

homeodomain proteins (uppercase letters at the ends of branches) fall into two groups, with representatives from each kingdom in each group That means, for example, the mouse homeodomain protein PAX6 is more closely related to fungal and flowering plant proteins, such as PHO2 and GL2, than it is to the mouse protein MEIS.

1.1 The Science of Life

Biology unifies much of natural science.

The study of biological systems is interdisciplinary because solutions

require many different approaches to solve a problem.

Life defies simple definition.

Although life is difficult to define, living systems have seven characteristics

in common They are composed of one or more cells; are complex and

highly ordered; can respond to stimuli; can grow, reproduce, and transmit

genetic information to their offspring; need energy to accomplish work;

can maintain relatively constant internal conditions (homeostasis); and are

capable of evolutionary adaptation to the environment.

Living systems show hierarchical organization.

The hierarchical organization of living systems progresses from atoms

to the biosphere At each higher level, emergent properties arise that are greater than the sum of the parts.

1.2 The Nature of Science

At its core, science is concerned with understanding the nature

of the world by using observation and reasoning.

Much of science is descriptive.

Science is concerned with developing an increasingly accurate description of nature through observation and experimentation.

Chapter Review

types in time and space, leading to changes over developmental

time into different tissue types—even though all cells in an

organ-ism carry the same genetic information

Living systems are able to collect information about the

environment, both internal and external, and then respond to

this information As you are reading this page, you are

famil-iar with this process, but it also occurs at the level of cells, in

terms of both single-celled organisms and the cells of

multi-cellular organisms Cells acquire information about their

en-vironment, send and receive signals, and respond to all of this

information with signal transduction systems that can change

cell morphology, behavior, or physiology (the subject of

chapter 9)

Evolution explains the unity and diversity of life

Biologists agree that all organisms alive today on Earth

descend-ed from a simple cellular organism that arose about 3.5 bya

Some of the characteristics of that organism have been preserved

through evolutionary history into the present The storage of

he-reditary information in DNA, for example, is common to all

living things

The retention of these conserved characteristics in a long

line of descent implies that they have a fundamental role in the

success of the organism A good example is provided by the

homeodomain proteins, which are critical regulators of the

pro-cess of development Conserved characteristics can be seen in

approximately 1850 homeodomain proteins, distributed among

three kingdoms of organisms (figure 1.13) The homeodomain

proteins are powerful developmental tools that evolved early;

they have been used and modified to provide new forms

The unity of life that we see in certain key characteristics

shared by many related life-forms contrasts with the incredible

di-versity of living things in the varied environments of Earth The

underlying unity of biochemistry and genetics argues that all life

evolved from the same origin event The incredible diversity of life

we see today arose by evolutionary change, much of it visible in

the fossil record

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Science uses both deductive and inductive reasoning.

Deductive reasoning applies general principles to predict specific results

Inductive reasoning uses specific observations to construct general

scientific principles.

Hypothesis-driven science makes and tests predictions.

Hypotheses are based on observations, and generate testable predictions

Experiments involve a test where a variable is manipulated, and a control

where the variable is not manipulated If the predictions cannot be

verified the hypothesis is rejected.

Reductionism breaks larger systems into their component parts.

Reductionism attempts to understand a complex system by breaking

it down into its component parts It is limited because parts may act

differently when isolated from the larger system.

Biologists construct models to explain living systems.

A model provides a way of organizing our thinking about a problem;

models may also suggest experimental approaches.

The nature of scientific theories.

Scientists use the word theory in two main ways: as a proposed

explanation for some natural phenomenon and as a body of concepts that

explains facts in an area of study.

Research can be basic or applied.

Basic research extends the boundaries of what we know; applied research

seeks to use scientific findings in practical areas such as agriculture,

medicine, and industry.

1.3 An Example of Scientific Inquiry: Darwin

and Evolution

Darwin’s theory of evolution shows how a scientist develops a

hypothesis and sets forth evidence, as well as how a scientific theory

grows and gains acceptance.

The idea of evolution existed prior to Darwin.

A number of naturalists and philosophers had suggested living things had

changed during Earth’s history Darwin’s contribution was the concept of

natural selection as a mechanism for evolutionary change.

Darwin observed differences in related organisms.

During the voyage of the H.M.S Beagle, Darwin had an opportunity to

observe worldwide patterns of diversity.

Darwin proposed natural selection as a mechanism for evolution.

Darwin noted that species produce many more offspring than will

survive and reproduce He observed that traits can be changed by

artificial selection Darwin proposed that individuals possessing traits that increase survival and reproductive success become more numerous in populations over time Darwin called this descent with modification (natural selection) Alfred Russel Wallace independently came to the same conclusions.

The predictions of natural selection have been tested.

Natural selection has been tested using data from many fields

Among these are the fossil record; the age of the Earth, determined

by rates of radioactive decay to be 4.5 billion years; genetic experiments showing that traits can be inherited as discrete units;

comparative anatomy and the study of homologous structures;

and molecular data that provide evidence for changes in DNA and proteins over time

Taken together, these findings strongly support evolution by natural selection No data to conclusively disprove evolution have been found.

1.4 Core Concepts in Biology

We use core concepts to organize information about the world around us

We introduce five core concepts to be used throughout this book, to help organize your thinking.

Life is subject to chemical and physical laws.

All living systems function based on the laws of chemistry and physics.

Structure determines function.

The function of macromolecules is dictated by and dependent on their structure Similarity of structure and function may indicate an evolutionary relationship.

Living systems transform energy and matter

Living systems have a constant need for energy, which is ultimately provided by the sun The nature of life is to constantly transform energy

We break down food molecules to provide energy to build up complex structures.

Living systems depend on information transactions

Hereditary information found in the DNA molecule is passed

on from one generation to the next This information is read out

to produce proteins, which themselves have information in their structures Living systems can also acquire information about their environment.

Evolution explains the unity and diversity of life

The underlying similarities in biochemistry and genetics support the contention that all life evolved from a single source The diversity found

in living systems arises by evolutionary change.

2 The process of inductive reasoning involves

a the use of general principles to predict a specific result.

b the generation of specific predictions based on a belief system.

c the use of specific observations to develop general principles.

d the use of general principles to support a hypothesis.

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3 A hypothesis in biology is best described as

a a possible explanation of an observation.

b an observation that supports a theory.

c a general principle that explains some aspect of life.

d an unchanging statement that correctly predicts some aspect

of life.

4 A scientific theory is

a a guess about how things work in the world.

b a statement of how the world works that is supported by

experimental data.

c a belief held by many scientists.

d Both a and c are correct.

5 The cell theory states that

a cells are small.

b cells are highly organized.

c there is only one basic type of cell.

d all living things are made up of cells.

6 The molecule DNA is important to biological systems because

a it can be replicated.

b it encodes the information for making a new individual.

c it forms a complex, double-helical structure.

d nucleotides form genes.

7 The organization of living systems is

a linear with cells at one end and the biosphere at the other.

b circular with cells in the center.

c hierarchical with cells at the base, and the biosphere at the top.

d chaotic and beyond description.

8 The idea of evolution

a was original to Darwin.

b was original to Wallace.

c predated Darwin and Wallace.

d Both a and b are correct.

A P P LY

1 What is the significance of Pasteur’s experiment to test the germ

hypothesis?

a It proved that heat can sterilize a broth.

b It demonstrated that cells can arise spontaneously.

c It demonstrated that some cells are germs.

d It demonstrated that cells can arise only from other cells.

2 Which of the following is NOT an example of reductionism?

a Analysis of an isolated enzyme’s function in an experimental

d An evaluation of the overall behavior of a cell

3 How is the process of natural selection different from that

of artificial selection?

a Natural selection produces more variation.

b Natural selection makes an individual better adapted.

c Artificial selection is a result of human intervention.

d Artificial selection results in better adaptations.

4 If you found a fossil for a modern organism next to the fossil of a dinosaur, this would

a argue against evolution by natural selection.

b have no bearing on evolution by natural selection.

c indicate that dinosaurs may still exist.

d Both b and c are correct.

5 The theory of evolution by natural selection is a good example of how science proceeds because

a it rationalizes a large body of observations.

b it makes predictions that have been tested by a variety

of approaches.

c it represents Darwin’s belief of how life has changed over time.

d Both b and c are correct.

6 In which domain of life would you find only single-celled organisms?

a Eukarya

b Bacteria c Archaead Both b and c are correct.

7 Evolutionary conservation occurs when a characteristic is

a important to the life of the organism.

b not influenced by evolution.

c no longer functionally important.

d found in more primitive organisms.

2 The classic experiment by Pasteur (figure 1.4) tested the hypothesis that cells arise from other cells In this experiment cell growth was measured following sterilization of broth in a swan-necked flask or

in a flask with a broken neck.

a Which variables were kept the same in these two experiments?

b How does the shape of the flask affect the experiment?

c Predict the outcome of each experiment based on the two hypotheses.

d Some bacteria (germs) are capable of producing heat-resistant spores that protect the cell and allow it to continue to grow after the environment cools How would the outcome of this experiment have been affected if spore-forming bacteria were present in the broth?

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C O N N E C T I N G T H E C O N C E P T S

This feature is intended to give you practice in organizing information using core concepts We use a metaphor of gears and cogs to represent a

conceptual hierarchy with each core concept represented as a gear Secondary concepts are the cogs, and tertiary concepts, which are particular

examples from the chapter, are presented as a list of bulleted points Using the completed conceptual Evolution explains the unity and diversity of life unit as a guide, build a list of examples from the chapter that illustrate how the secondary concept “Natural selection is a mechanism for evolution”

supports the core concept “Evolution explains the unity and diversity of life.”

Evolution explains the unity and diversity of life

Natural selection is a mechanism for evolution

Life’s diversity is overwhelming

• Living systems are organized hierarchically.

• Living systems are composed of cells, which are organized into tissues, which are organized into organs.

• Evolution has resulted in incredible diversity of life, from single-celled bacteria to multicellular plants and animals.

• Classifying organisms based on morphological and molecular characteristics led to two unicellular domains and a third domain composed of more complex single-celled and multicellular organisms.

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