tạo hoa hồng xanh bằng kĩ thuật gene

sơ lược kĩ thuật tạo hoa hồng xanh bằng kĩ thuật gene RNAi, hoa hông xanh có giá trị kinh tế cao tuy nhiên công nghệ này đang gặp phải vấn đề là kĩ thuật này vẫn chưa tạo ra một bông hồng xanh thật sự

BLUE ROSE TRANSGENIC

PLANT

Agenda Style

Introduction 01

method of gene transfer 02

applying 03

Van Pham Nhu Ngoc

student

618H0045@gmail.com 0587192774

Our Team

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Ph.D Tran Thi Dung

lecture

Introduction

02

The blue rose is a flower of the genus Rosa

(family Rosaceae) with the prefix from blue

to purple

 In 2004, researchers used genetic modification to

create roses that contain the blue pigment

delphinidin

What is

RNAi?

similar to that of the gene inactivating agent (short strands of RNA about 21-27 nucleotides)

RNAi is involved in controlling gene activation in the post-transcription phase

Basic features of the RNAi technique

 Specificity, high efficiency, very sensitive.

 Inactivates genes of individuals at different stages, inherited from generation to generation,

tissue to tissue.

 The dsRNA design is easy if the genome sequence is known.

Method of gene transfer

W EA KN

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ST RE

NG TH S

OP PO RT UN IT

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TH RE AT S

Application of RNAi technology for

gene replacement in plants

An iris gene for an enzyme, DFR, required to

complete the delphinidin-synthesis reaction.

remove the gene encoding the enzyme

created the 'silencer' gene to exploit a cellular phenomenon called RNA

interference (RNAi)

SWOT

Creating blue roses using RNAi technique

CHU TRÌNH HÌNH THÀNH SẮC TỐ TRÊN CÂY TRỒNG

Anthocyanin dihydrokaempferol enzyme controls

all three cycle of plant pigment formation

including: cyanidin, pelargonidin and delphinidin

 RNAi technique to inhibit the activity of the DFR gene in red roses  leads to inhibition of

 complete the delphinidin synthesis cycle.

=> using RNAi technique to inhibit the DFR gene  clone a new delphinidin gene from pansy and DFR gene from iris flower  DFR gene inhibition of roses without affecting the DFR gene of iris flowers

5 Influence of

pH

6 3

2

1

Petal ph cell concentrations are often genetic

Ordinary rose petals have a pH concentration of about 4.5.

Scientists have just come up with RNAi gene-suppressing techniques to identify genes that affect petal acidity or adjust petal color in other directions.

Cell pH is one of the main reasons flowers have

the same anthocyanin cycle but have different

colors

While the cell pH is alkaline, the pigment

of anthocyanin usually becomes greener.

Soil pH has little or no effect on the

ph of the petal cells.

3 Applying

The market for green roses is mainly in: America,

Australia and Japan

04

In addition to creating a blue rose, the scientist also creates a variety of flowers with different colors, fragrances and perils

05

The application of RNAi technology not only in creating blue

roses but also opens up a new era for the application of

genetic engineering in agriculture.

06

In the global commission business with sales of

1.2 billion USD / year

01

Unique flower means making huge

profits.

02

The demand for roses, cuttings accounts for 50% of the flower market.

03

Thank You

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