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ĐÁNH GIÁ HIỆU QUẢ XỬ LÝ BÙN THẢI SNH HỌC VÀ CHẤT THẢI NẤM BẰNG GIUN QUẾ PERIONYX EXCAVATUS

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EFFECTIVENESS ASSESSMENT TREATMENT OF BIOSOLIDS WASTE AND

MUSHROOM WASTE BY THE SPECIES PERIONYX EXCAVATUS

Nguyen Thi Thu Phuong 1* , Sharon Melissa Pineda Castillo 2

1

University of Technology - TNU

2 Universidad de Ciencias Aplicadas y Ambientales, Colombia

ABSTRACT

The efficiency of earthworms in decomposing different substances has been studied over the years,

to assess earthworm’s ability and efficiency at vermicomposting process to treat natural and anthropogenic waste The present study was settled in boxes under controlled conditions with two

types of waste (biosolids and mush room waste) Perionyx excavatus treated biosolids waste from

Yen Binh Industry Zones in Thai Nguyen, and it was also used in the three samples with 3 concentrations Measures were taken regarding vermicomposting speed, and parameters as C, N,

P, K, pH and moisture The results showed that earthworm’s treatment increased all the nutrients

-N, P and K mainly- in every tested sample, and a better efficiency in the blend of mushroom and biosolids 1:2 (MB2) It was concluded that using vermicomposting to treat biosolids waste along with mushroom waste, it can increase the nutrients of the final product and that it is feasible to use

biosolid waste mixed with mushroom waste to treat organic material with P excavatus

Key words: Vermicomposting, waste, P excavatus, biosolids, mushroom

INTRODUCTION*

Biological treatments for wastewater and

organic waste have been highly used because

of its effectiveness and low-cost, if compared

to treatment plants’ installation and operation

costs [2] The most well-known biological

process using earthworms is composting and

vermicomposting for natural and

anthropogenic waste Vermicomposting is a

process of bioxidation, digestion,

mineralization and stabilization of organic

material using the interaction of earthworms

and microorganisms The later ones are

responsible for the biochemical degradation

of organic material; however, earthworms are

the crucial drivers of the whole process due to

the fragmentation and conditioning of the

substrate, which is modified in its biological

activity The earthworms also mantains

aerobic condition in the organic waste, ingest

the solid waste and then transform it into

earthworm’s biomass and respiration

products; finally they expel the stabilized

product that it’s called vermicompost During

vermicomposting process, nutrient’s presence

*

Tel: 0984 094259, Email: thuphuong0709@gmail.com

in the original feed material, such as N, P, K and Ca, are turn to be more soluble and available to plants Vermicomposting and treatment plants create a treated and stabilazed product called biosolid [3]

According to Singh & Suthar (2008), vermicompost product is excellent due to the reduction of contaminants, the ability to store nutrients for long term and its homogenety [10] Using earthworms for vermicomposting can provide cheaper solutions for social, economical and environmental issues in human society As vermicomposting process

is simple, it doesn’t require extra technology

so that it can be used and implemented by farmers [4] The most know earthworms used

in vermicomposting are Eisenia fetida,

Lumbricus rubellus, E Andrei and Peronyx excavatus, because of their rates in degrading

numerous organic waste material and that they can consume at least half of their body weight in a week Also, it has been suggested

a potential accumulation of heavy metals in their bodies [12] In order to maintain dynamic equilibrium and regulate soil fertility, earthworm’s environment should be adequate in moisture, soil texture, pH,

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electrolyte concentration, and enough food or

samples to feed themselves [13]

Earthworms have been used to create compost

of better quality for landfills from pig manure

cow dung, sheep manure and even tomato,

rice (Pui, et al., 2014) and mushroom (Abu, et

al., 2011), as it has been proved that

vermicompost product can increase mineral

nutrients, plant growth rate and humic acids

it can be used in crops and landfills [1, 8]

The aim of the project was to identify the

performance of P excavatus in decomposing

different concentrations of samples with

mushroom waste mixed with biosolids, to

determinate if mushroom waste mixed with

biosolids can be used to replace cow dung and

biosolids alone or not

MATERIALS AND METHODS

Materials and testing

Materials

Perionyx excavatus was chosen for its fast

growth and reproduction [7] in the study were

cultivated for four months at a local farm in

Dong Hy district, Thai Nguyen province

During that time, they were fed with cow

dung mixed with water Biosolids from Yen

Binh Industry Zones located in Thai Nguyen

were taken and mixed at different

concentrations with mushrooms (Table 3.2)

from Thai Nguyen University of Agriculture

and forestry (TNU-University of Agriculture

and Forestry) to test the effectiveness of

earthworms under different concentrations

Mushrooms’ samples contain an admixture of

100 kg sawdust, 4 kg corm bran, 4 kg rice

bran and 1.5 kg CaCO3

Five samples were treated using the following

concentrations: cow dung (CD), mushroom

waste (M1), mushroom mixed with biosolids

(mushroom:biosolids following the rate 1:1,

1:2, 1:3 (MB1, MB2, MB3) were tested by

earthworms In this study, biosolids were

taken from the sludge filter pressing and

drying process in Yen Binh’s wastewater

treatment system After the treating process

by earthworms, parameters as K, P, N, C, pH, moisture of vermicompost of five samples (CD, M1, MB1, MB2, MB3) were analyzed in Monitoring Centre for Natural Resources and Environment and Thai Nguyen University of Technology’s laboratory Besides, the characteristics of mushroom waste before treating by earthworm (M0), biosolids before treating by earthworm (B0) were also analyzed This was used to compare the quality and speed

of vermicomposting process

Testing

The samples were settled in boxes of expanded polystyrene (EPS), under controlled water and light conditions The boxes were of the same size (40x20x7 cm); on the bottom of them there was a plastic cover that held sand, soil and a composition of Effective Microorganisms (E.M); this was used to increase the speed of vermicomposting On top was a piece of white canvas, at first, that contains 1.5 kg earthworm and vermicompost (V0), then, adds 0.6 kg the food of mushroom, biosolids or cow dung that were raised the humidity by 50 ml E.M, 150 ml water When earthworms eat all of the food, the treating time and the mass of earthworm and vermicompost (EV1) were determined

Laboratory methods

Methods used in the laboratory to identify the parameters C, N, P, K, pH, and moisture were following TCVN 5979-2007, 10TCN 302:2005, 10 TCN 366:99, TCVN 8562:2010, TCVN 8557:2010 and TCVN 8563:2010, respectively

RESULTS AND DISCUSSION

Characteristics of vermicomposting

The results show a significant increase of all nutrients in the mushrooms sample after the earthworm’s presence and treatment, being N and P the ones which increased the most, without disregarding the increased in C and

K Despite this, the values of nutrients are far from be as good as cow dung after being treated by earthworms

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Table 1 Characteristics of vermicomposting, biosolids and mushroom waste

Table 2 Mass of vermicompost and time to treat

Samples

V 0

(kg)

Cow dung (kg)

Mushroom (kg)

biosolids (kg)

EM (ml)

Water (ml)

Total weight

EV 0 (kg)

EV 1 (kg)

Time to treat (days)

The samples of biosolids mixed with different

mushroom’s concentrations show an important

increase in all the nutrients tested, if compared

with the data of biosolids itself It is show that

MB1 has the highest rate of K than MB2 and

MB3 samples and that MB3 sample has the

highest rate of P; but it was MB2 sample that

showed the best rate of both C and N nutrients

All the samples show a pH between 7.2 and

7.6, which are in the recommended rate for

compost according to Bord Na Móna (2003)

[4] In contrast, according to Sweeten &

Auvermann, (2011) the moisture must be

between 40% - 60% and only sample that

meets the parameter is MB2 sample [11]

The study showed that vermicomposting

process increase nutrients as N, P, and K, just

as Pattnaik & Reddy, (2010) stated in their

study using the P excavatus [7] This

increasing richness in nutrients was also

register by Deka et al., (2009) in his study [5]

It was also state that earthworms preffered

cow dung because of its rich substrate; this

was also evidenced in the present study as

cow dung sample was eaten faster than the

others and it was the sample with the highest

rate of nutrients Taking this into account,

cow dung can be used widely in Indian’s

farms (Rajpal, Bhargava, Chopra, & Kumar,

2013) [9] as well as Vietnam, because it

doesn’t require extra technology (Deka et al, 2009) [5] and nutrients are easily absorbed by plants (Kaushik & Garg, 2004) [6]

As good as vermicomposting can be in Vietnam’s farms, the aim of this study is to offer another option in the cities of the country by using mushroom and biosolids Cow dung isn’t as available and as cheap as mushroom waste and biosolids in Vietnam’s

cities, and those wastes are also effective with

P excavatus as it is shown in this study, and

as it is supported by Abus’ (et al., 2011) study

in which was found that by using MB2 of sewage sludge:mushroom compost, could be used for vermicompost [1] That concentration was also one of the best, if not the best, of the present study, and thus MB2 of mushroom waste:biosolids can also be used for vermicomposting The other samples had also high performance, but taking into account the time, nutrients and moisture, it can be said that MB2 of mushroom waste:biosolids is the best sample to be used Comparing with EV0, EV1 reduces from 0.35

to 0.5 kg because of humidity process of water and absorbtion of the sand

Alternatively, P excavatus demonstrated a

preference of cow dung by showing the best decomposition speed in contrast with the other samples, especially treated mushroom,

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which took 12 days more than cow dung

sample becoming the slowest of all

Considering the number of days and the

concentrations of the samples, the earthworms

showed a better performance with MB2,

follow by MB3 sample

Although MB2 of mushroom waste:biosolids

is between the moisture parameter, the rest of

the samples don’t fullfill this requirement,

including cow dung sample; this must be sort

in order to give the earthworms an optimmal

environment for its growth and to achieve the

fastest rate in vermicompost [7] There is an

specific need in extending and elaborating

information about heavy metals in biosolids,

as it is another main concern when using

biosolids mixed with mushroom waste for

land crops Nevertheless, Abu (et al., 2011)

stated that in an environment of mushroom

waste with sewage sludge, earthworms can

decrease the concentration of heavy metals

yet earthworms would need perhaps more

than 80 days [1] Microorganisms presence is

another important parameter to be tested

CONCLUSION

Regarding parameters as moisture, pH,

nutrients and time of vermicompost, MB2

reaches the optimal time to treat ( seven days)

and with the quality of vermicompost (C:

25,860mg/kg; N: 5,054 mg/kg; P: 7,716

mg/kg; K: 3.9636 mg/kg and moisture:

54.46%) can be easily used with

environmental and social advantages and it

might be another option of vermicomposting

without relying exclusively on cow dung;

However, there must be an investigation

about heavy metals and microorganisms after

vermicomposting process in order to use the

final product in field crops without health and

environmental consequences.

REFERENCES

1 Abu, A B., Noor, Z M., Teixeira da Silva, J.,

Abdullah, N., & Ainurzaman, A J (2011),

"Vermicomposting of sewage sludge by

Lumbricus rubellus using spent mushroom

compost as feed material: Effect on concentration

of heavy metals", Biotechnology and Bioprocess Engineering, 16, 1036-1043

2 Amouei, A., Yousefi, Z., Khosravi, T (2017),

"Comparison of vermicompost characteristics produced from sewage sludge of wood and paper

industry and household solid wastes", Journal of Environmental Health Science and Engineering,

3 Artuso, N., Schmidt, O., Connery, J., Kennedy, T., Grant, J (2011), "Assessment of biosolids in earthworm choice tests with different species and

soils", Global Nest Journal, 13(3), 255–265

4 Bord Na Móna;., (2003), "Analysis of greenwaste compost from Dublin City Council and interpretation

of results", Dublin: Bord Na Móna

5 Deka, H., Deka, S., Baruah, C (2009),

"Vermicomposting of Water Hyacinth Eichhornia (Eichhorina crassipes) through vermicomposting

by native earthworm Perionyx sp", Proceedings of International symposium on Environmental Pollution, Ecology and Human Health, 53-55

5 Gutiérrez, F., Borraz, J., Montes, J., Nafate, C., Archila, M., Oliva, M., Dendooven, L (2007),

"Vermicompost as a soil supplement to improve growth, yield and fruit quality of tomato (Lycopersicum esculentum)", Bioresource Technology, 98(15), 2781-2786

6 Kaushik, P., Garg, V (2004), "Vermicomposting

of mixed solid textile mill sludge and cow dung with the epigeic earthworm Eisenia foetida",

Bioresource Technology, 311-316

7 Pattnaik, S., Reddy, M (2010), "Nutrient status

of vermicompost of Urban green waste processed

by three earthworm species - Eisenia fetida, Eudrilus eugeniae, and Perionyx excavatus (P

University, Ed.)", Applied and Environmental Soil Science, 13

8 Pui, K., Yeong, T., Lin, S., Ai, C L (2014),

"Sustainable reuse of rice residues as feedstocks in vermicomposting for organic fertilizer

production", Environ Sci Pollut Res, 1349–1359

9 Rajpal, A., Bhargava, R., Chopra, A., Kumar, T (2013), "Vermistabilization and nutrient enhancement of anaerobic digestate through earthworm species Perionyx excavatus and

Perionyx sansibaricus", Springer Japan, 219–226

10 Singh, S., Suthar, S (2008),

"Vermicomposting of domestic waste by using two epigeic earthworms (Perionyx excavatus and

Perionyx sansibaricus)", International Journal of Environmental Science and Technology, 99-106

11 Sweeten, J M., Auvermann, B W (2011),

"Composting Manure and Sludge", Texas: The Texas A&M University System Obtenido de AgriLIFE EXTENSION Texas A&M System

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12 Umamaheswari, S., Selvanayagam, S (2015),

"Vermiremdiation of Sugar Industry Waste using

Earthworms Eudrilus eugeniae,Perionyx excavatus

and Eisenia fetida", European Journal of

Zoological Research, 28-36

13 Zigmontiene, A., Liberytė, I (2014), "Heavy Metals (Cr, Cd And Ni) Concentrations in Sewage Sludge and Bioaccumulation By Californian Earthworms in the Process of Vermicomposting",

The 9th International Conference "Environmental Engineering 2014, 22-23

TÓM TẮT

ĐÁNH GIÁ HIỆU QUẢ XỬ LÝ BÙN THẢI SNH HỌC VÀ CHẤT THẢI NẤM

BẰNG GIUN QUẾ PERIONYX EXCAVATUS

Nguyễn Thị Thu Phương 1* , Sharon Melissa Pineda Castillo 2

1 Trường Đại học Kỹ thuật Công nghiệp

2

Universidad de Ciencias Aplicadas y Ambientales, Colombia

Hiệu quả của giun Quế trong việc phân hủy các chất thải khác nhau đã được nghiên cứu trong nhiều năm, đánh giá khả năng và hiệu quả của giun trong quá trình ủ phân xử lý chất thải Nghiên cứu này đã được thực hiện trong các thùng xốp ở điều kiện kiểm soát với hai loại chất thải (bùn thải sinh học và chất thải nấm) Perionyx excavatus xử lý bùn thải sinh học từ nhà máy xử lý nước thải thuộc khu công nghiệp Yên Bình, Thái Nguyên, và nó cũng được sử dụng kết hợp với bã thải nấm rơm trong ba mẫu với 3 nồng độ khác nhau Tiến hành đo và xác định các thông số như C, N, P,

K, pH và độ ẩm Kết quả cho thấy rằng việc xử lý chất thải bằng giun làm tăng tất cả các chất dinh dưỡng trong mỗi mẫu, và đạt hiệu quả tối ưu ở tỷ lệ bã thải nấm: bùn thải sinh học là 1: 2 Sử dụng giun để xử lý bùn thải sinh học cùng với chất thải nấm, nó có thể làm tăng chất lượng phân bón

Từ khóa: Phân giun, chất thải, P excavatus, bùn thải sinh học, nấm

Ngày nhận bài: 01/11/2017; Ngày phản biện: 15/11/2017; Ngày duyệt đăng: 05/01/2018

*

Tel: 0984 094259, Email: thuphuong0709@gmail.com

Ngày đăng: 15/01/2021, 01:32

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