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Asymmetric synthesis of (R)-2-[3-(methoxymethoxy)propyl]- 3,3-diphenyl-1-tosyl-1,3-azasilinan-6-one

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Azasilanes have excellent bioactivities; however, asymmetric synthesis of azasilinan-6-one with a substitution at the second potion has been investigated very little. In this paper, (R)-2-[3-(methoxymethoxy)propyl] -3,3-diphenyl-1-tosyl-1,3-azasilinan-6-one was successfully synthesized.

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This paper is available online at http://stdb.hnue.edu.vn

ASYMMETRIC SYNTHESIS OF

(R)-2-[3-(METHOXYMETHOXY)PROPYL]-3,3-DIPHENYL-1-TOSYL-1,3-AZASILINAN-6-ONE

Duong Quoc Hoan1 and Scott McN Sieburth2

1Faculty of Chemistry, Hanoi National University of Education

2Department of Chemistry, Temple University, the United States of America

Abstract. Azasilanes have excellent bioactivities; however, asymmetric

synthesis of azasilinan-6-one with a substitution at the second potion has

been investigated very little In this paper, (R)-2-[3-(methoxymethoxy)propyl]

-3,3-diphenyl-1-tosyl-1,3-azasilinan-6-one was successfully synthesized The

chiral center of silylsulfinamide was prepared by the addition of silyl lithium

to (R)-Davis’ chiral sulfinimine Cyclization of δ-amino carboxylic acid gave

1,3-azasilinan-6-one that can be an important product to synthesize 2-substituted

1,3-azasilinan rings Structures of all new compounds were confirmed by IR,1H

and13C-NMR, along with the exact mass

Keywords: Synthesis,

(R)-2-[3-(methoxymethoxy)propyl]-3,3-diphenyl-1-tosyl-1,3 azasilinan-6-one, IR,1H and13C-NMR

1 Introduction

Nitrogen-containing heterocycles constitute structural frameworks in a plethora

of pharmaceuticals and alkaloids and are essential components of the pharmacophore [2, 3, 6] In recent years, there has been an interest in the preparation of heterocycles

in which one of the ring carbons has been replaced by silicon atoms A few bioactive silicon-nitrogen heterocycles have thus far been synthesized and screened for bioactivity

For example, the dopamine receptor antagonist silahaloperidol 1 (Figure 1) displays

improved selectivity compared to haloperidol Furthermore, its metabolic fate in human liver microsomas does not produce a silicon analogue of the neurotoxic metabolite HPP+ (Hereditary pyropoikilocytosis), which is responsible for the severe side effects of haloperidol [4] Other heterocyclic sila analogues have been prepared, including a class

of spirocyclic acceptor ligands, such as 2 [5] the neurotropic tetrahydroisoquino-line sila

Received January 2, 2014 Accepted August 15, 2014.

Contact Duong Quoc Hoan, e-mail address: hoanqduong@gmail.com

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analogue 3 [5] and the silicon analogue 4 [9] of the anti-depressive agent dimetracrine

(Figure 1) Another important compound in the biorganosilicon area is a sila analogue of

proline prepared enantioselectively by Vivet et al [8].

Figure 1 Examples of bioactive silicon-nitrogen heterocycles

Annaliese K Franz et al have a detail review of advantages gained when carbons

are replaced by silicon for bio-activities and medicinal properties of silicon containing compounds Because it is larger than carbon, the silicon plays an important role in increasing lipophilicity, and flexibilities of organosilicon molecules often enhances cell and tissue penetration and alter the potency and selectivity of the silicon structure relative

to the carbon structure [1]

Scheme 1 Retrosynthesis of 1,3-azasilinan-6-one (5)

Different approaches have been developed to prepare such heterocyclic systems containing silicon and nitrogen [1, 8] However, few syntheses have targeted 2-substituted 1,3-azasilaheterocycles These include the reaction of a bishaloalkylsilane with a primary amine and a nonregioselective aminomercuration which yielded a 2-substituted azasilinane as a by-product [1] In this paper, a flexible and efficient approach to the stereo controlled synthesis of a 2-substituted-1,3-azasilinan-2-one is reported The synthetic

route is outlined in Scheme 1, whereby the azasilaheterocycle 5 can be formed by an intra

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molecular cyclization, respectively The substrates 6 would be prepared from sulfinimine

7 and silyl lithium would be made from lithiation of 8, a strategy recently and successfully

developed by Sieburth’s group

2 Content

2.1 Experiment

All IR spectra were recorded on a Mattson 4020 GALAXY series FT-IR (Germany) NMR spectra were studied on 400 Bruker and Avance III 500 spectrometers (Germany) The Perkin Elmer Model 341 Polarimeter was used to obtain optical rotations

* 3-(Diphenylsilyl)propan-1-ol (8)

To a solution of diphenylsilane (9) (10.0 g, 54.3 mmol) in heptane (150 mL) was

added allylic alcohol (5.5 mL, 65.2 mmol), tert-dodecylmercaptan (1.2 mL, 5.4 mmol)

and AIBN (0.44 g, 0.26 mmol) The resulting mixture was heated to 75C for 19 h, and then concentrated in vacuo Flash column chromatography using a gradient eluent (100:1

to 95:5 hexane and ethyl acetate) gave alcohol 8 (11.2 g, 80%) as a colorless oil Rf = 0.6 (hexane/ethyl acetate 6:1) Rf = 0.30 (4: 1 hexane/ethyl acetate) IR (neat) 3337 (broad),

2931, 2119, 1428 cm−1;1H-NMR (400 MHz, CDCl3) 7.54 - 7.59 (m, 4H), 7.34 - 7.43

(m, 6H), 4.88 (t, J = 3.8 Hz,1H), 3.64 (t, J = 6.5 Hz, 2H), 1.67 - 1.76 (m, 2H), 1.27 (br,

1H), 1.14 - 1.21 (m, 2H);13C-NMR (125 MHz, CDCl3) 135.1, 134.2, 129.7, 128.1, 64.9, 27.5, 8.1

* (R)-N-1-[(3-hydroxypropyl)diphenylsilyl]-4-(methoxymethoxy)butyl-4-methylbenzene sulfonamide (6)

To a mixture of lithium (1.73 g, 247 mmol) in THF (20 mL) at 0C in dry argon gas

was added by-compound 8 (3.0 g, 12.4 mmol) The mixture was stirred at 0C until the reduction was completed The progress of reaction was monitored by NMR The solution then was cooled to -78C, transferred via a cannula to a solution of sulfinimine 7 (0.73

g, 4.1 mmol) in THF (10 mL) at -78 C for more than 15 min The resulting solution was stirred at -78C for 5 hr, gradually warmed up to room temperature and stirred for overnight The reaction was quenched by water (100 mL), and extracted by ethyl acetate (3× 30 mL) The organic combination phase was washed by water (3 × 50 mL), and then

dried over with Na2SO4, concentrated in vacou Flash column chromatography gave crude

sulfinamide 11 (1.3 g, 76%) The sulfinamide 11 (1.3 g, 3.1 mmol) was dissolved in DCM

(20 mL) at 0◦ C was added 77% m-CPBA (0.83 g, 3.72 mmol) The progress of reaction was monitored by TLC The excess m-CPBA was quenched by saturated Na2SO3 The mixture was extracted with DCM (3× 30 mL) Combination of organic phases was dried

over with Na2SO4, concentrated in vacou Flash column chromatography gave sulfone

6(1.45 g, 89%) as a colorless oil Rf = 0.58 (hexane/ethyl acetate 1:2); [α]20D = +9.4 (c

0.085, CHCl3); IR: 3289, 3068, 2926, 2874, 1598, 1540, 1155, 1111, 702 cm−1;1H-NMR

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(400 MHz, CDCl3): δ 7.66 (d, J = 7.4 Hz, 2H), 7.5 - 7.3 (m, 10H), 7.2 (d, J = 8.0 Hz, 2H), 3.56 - 3.50 (m, 1H), 3.46 (t, J = 6.5 Hz, 2H), 3.25 - 3.20 (m, 2H), 3.21 (s, 3H), 2.4

(s, 3H), 1.87 (br, 1H), 1.72 - 1.62 (m, 1H), 1.5 - 1.3 (m, 5H), 1.0 (m, 2H);13C-NMR (100 MHz, CDCl3): δ 143.2, 138.7, 135.7, 135.5, 132.6, 131.9, 130.1, 129.6, 128.3, 127.2,

96.2, 67.3, 65.2, 55.2, 42.0, 29.8, 29.1, 27.3, 26.6, 21.6, 7.45 Exact mass: [M - Na]+

calcd for [C28H37NNaO5SSi]+ 550.2054, found 550.2035

* (R)-3-[4-(Methoxymethoxy)-1-(4-ethylphenylsulfonamido)butyl] diphenylsilyl propa noic acid (22)

To a solution of sulfonamide 6 (1.0 g, 1.9 mmol) in a mixture of solvent

DCM/CH3CN/ water (1/1/1, 50 mL) was added RuCl3 (3.9 mg, 0.019 mmol), and then NaIO4 (1.6 g, 7.6 mmol) The resulting solution was stirred at room temperature for

an hour The progress of reaction was monitored by TLC The mixtures reaction was extracted with DCM (3× 30 mL), then the combination of organic phase was dried over

Na2SO4, concentrated in vacou Flash column chromatography gave acid 22 (0.7 g, 68%)

as a colorless oil Rf = 0.4 (tail) (hexane/ethyl acetate 2/1) [α]20

D = +12.0 (c 0.05, CHCl3) IR: 3205 - 2560 (br), 3284, 3070, 2883, 1705, 1592, 1111, 734 cm−1;1H-NMR (500 MHz, CDCl3): δ 7.66 (d, J = 8.2 Hz, 2H), 7.5 - 7.3 (m, 10H), 7.2 (d, J = 7.8 Hz, 2H), 4.56 (d,

J = 9.4 Hz, 1H), 4.44 (s, 3H), 3.57 - 3.51 (m, 1H), 3.17 - 3.19 (m, 2H), 3.22 (s, 3H), 2.3

(s, 3H), 2.22 - 2.17 (m, 1H), 1.71 - 1.62 (m, 1H), 1.46 - 1.40 (m, 1H), 1.37 (dd, J = 8.6,

4.2 Hz, 1H), 1.36 - 1.30 (m 3H); 13C-NMR (125 MHz, CDCl3): δ 179.5, 143.4, 138.6,

135.6, 135.5, 131.7, 131.1, 130.4, 129.7, 128.5, 127.1, 96.2, 67.3, 55.2, 41.9, 29.1, 28.2, 27.3, 21.6, 6.5; Exact mass: [M - Na]+ calcd for [C28H35NNaO6SSi]+ 564.1847, found 564.1819

* (R)-2-[3-(Methoxymethoxy)propyl]-3,3-diphenyl-1-tosyl-1,3-azasilinan-6-one (5)

To solution of acid 22 (0.1 g, 0.18 mmol) in THF (4mL) at -20C was added triethyl

amine (63 µL, 0.45 mmol) followed by PivCl (22 µL, 0.18 mmol) The solution was stirred

at -20◦ C for an h, and then added LiCl (11.3 mg, 0.27 mmol) followed (S)-oxazolidione

(25.5 mg, 0.2 mmol) The mixture was stirred at the same temperature for an hour, and then at 0C for 2 h, quenched with saturated NH4Cl (5 mL), extracted with ethyl acetate (3 × 5 mL) The combined organic layers were washed brine (10 mL), dried over with

Na2SO4, and concentrated in vacou Column chromatography gave 5 (90 mg, 92%) R f

= 0.7 (hexane/ethyl acetate 1/1); [α]20

D = +47.0 (c 0.39, CHCl3); IR: 3070, 3012, 2926,

2883, 1694, 1591, 1343, 1107, 715 cm−1;1H-NMR (400 MHz, CDCl3): δ 7.7 (d, J = 8.0

Hz, 2H), 7.6 - 7.3 (m, 10H), 6.98 (d, J = 8.0 Hz, 2H), 4.89 - 4.84 (m, 1H), 4.5 (s, 2H),

3.48 - 3.40 (m, 2H), 3.22 (s, 3H), 2.93 - 2.79 (m, 2H), 2.3 (s, 3H), 1.86 - 1.72 (m, 3H),

1.65 (ddd, J = 15.3, 6.0, 4.0 Hz, 1H), 1.62 - 1.54 (m, 1H), 1.36 (ddd, J = 15.3, 13.3, 7.2

Hz, 1H);13C-NMR (100 MHz, CDCl3): δ 173.2, 144.2, 136.4135.2, 135.2, 132.6, 132.3,

130.7, 129.1, 128.9, 128.8, 128.6, 96.3, 67.0, 55.2, 45.2, 33.8, 30.4, 28.3, 21.7, 4.3; Exact mass: [M - Na]+calcd for [C28H33NNaO5SSi]+546.1741, found 546.1724

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2.2 Synthesis, results and discussion

Alcohol 8 was synthesized from diphenylsilane (9) and allylic alcohol in 80% yield.

It is noteworthy that the synthesis of alcohol 8 can be scaled up to 100 g of diphenylsilane (9) (Scheme 2).

Scheme 2 Radical chain hydrosilylation of allylic alcohol

In general, radical chain hydrosilylation of alkenes using R3SiH is not very helpful, since the hydrogen abstraction step is slow under standard experimental conditions; however these reactions can be promoted under milder conditions by the presence of catalytic amounts of a thiol [12] Thus, the thiol acts as the catalyst and the H transfer agent in propagation steps (Scheme 3) Under high temperature of the initiation step, AIBN (azobisisobutyronitrile) is decomposed to eliminating a molecule of nitrogen gas

to form two 2-cyanoprop-2-yl radicals (reaction 1, Scheme 3) The radical reacts with thiol XSH to yield thiyl radical (reaction 2, Scheme 3), and then the thiyl radical abstract

a hydrogen atom from the R3SiH The resulting R3Si radical adds to the double bond

to give a radical adduct, which then reacts with the thiol and gives the addition product together with ‘fresh’ XS radicals to continue the chain Chain reactions are terminated by radical-radical combination or disproportionation reactions, Scheme 3

Scheme 3 Propagation steps for radical-based hydrosilylation catalyzed by thiol

With large amount of the alcohol 8 in hand, the alcohol 8 was treated with lithium

metal at -78 C in tetrahydrofuran (THF) to make (Si,O)-dianion 10 The reaction

mixture was turned in black and released hydrogen gas The addition of (Si,O)-dianion

to sulfinimine 7 gave sulfinamide 11 in 75% yield as a crude product Due to instability

of sulfinamide 11 on silica gel, it was oxidized by m-CPBA (meta-chloroperoxybenzoic

acid) to give a stable sulfonamide 6 in 89% yield, Scheme 4.

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Scheme 4 Synthesis of sulfonamide 6

Mechanism of lithium (Si, O)-dianion 10 formation and similarities is still unclear Yingjian Bo and Scott Sieburth [10] proved that the first step of the lithiation of 12

(Scheme 5, part A) is a reduction of Si-O bond and open up the ring to form (Si-O)-dianion

13 After 4 h, an asymmetric and a symmetric alcohols, hydrolysis products of (O, O)-dianion and (Si, O)-dianion, were separated by a flash column chromatography To explain the formation of these two alcohols, the either (Si)-anion or (O)-anion substitutes alkoxy of silicon following two path ways a and b and form two correlative dianions

14 , and 15 These two dianions were reduced by lithium to yield only (Si, O)-dianion

16 [13] In our case (Scheme 5, part B), Li reacted with hydroxyl group of alcohol 8

to release hydrogen gas and (O)-anion 17 then cyclized (pathway a’) to form silafuran

18 The silafuran 18 was converted to (Si, O)-dianion 10 following the same manner of

chemistry in Scheme 5, part A On the other hand, lithium reacted with both hydroxyl

group and Si-H to produce the same (Si, O)-dianion 10 (path way b’), Scheme 5, part B.

Scheme 5 Lithiation of Si-O and Si-H bond cleavages

Absolute stereochemical assignment of the major isomer was inferred by analogy

upon X-ray diffractions studies of many related single crystals such as compound 19, and

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20[14-20] Both structures showed that the major isomer is the opposite isomer than the one predicted via the closed transition-state model suggested by Ellman for the majority

of organometallic additions Therefore, an open, acyclic transition state was proposed

to explain the stereochemical results where the nucleophin attacks to the lest sterically

hindered effect (Figure 2, 21).

Figure 2 Absolute stereochemical assignment of the major isomer

and proposed acyclic transition state

Alcohol 6 was oxidized by a stoichiometric amount of NaIO4and catalytic amount

of RuCl3 to give acid 22 in 68% yield Carboxylic group was activated by PivCl (Pivaloyl chloride) in basic condition of triethylamine to form 1,3-azasilinan-6-one 5 in 92% yield.

Scheme 6 Synthesis of 1,3-azasilinan-6-one

A proposed mechanism of cyclization is shown in Scheme 7 In the presence of triethyl amine, carboxylic and tosyl amine were deprotonated, therefore carboxylate reacts

easily with pivaloyl chloride (PivCl) to yield anhydride 23 following cyclization to give 1,3-azasilinan-6-one 5 in high yield.

Scheme 7 Proposed mechanism of cyclization reaction

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3 Conclusion

In conclusion, (R)-2-(3-(methoxymethoxy)propyl)-3,3-diphenyl-1-tosyl-1,3- azasili

nan-6-one (5) was synthesized successfully in five linear steps in 33% yield This

is an approach for the synthesis of 1,3-azasilaheterocycles with a substituent in the 2-position as analogs of cyclic alkaloids The synthesis involves hydridosilane lithiation and sulfinimine addition with good diastereoselectivity at the silicon bearing a stereogenic center, producing the desired compounds in excellent yield This result can be applied for synthesis of a sila analogue of natural product mimics

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[1] Annaliese K Franz, Sean O Wilson, 2013 Organosilicon Molecules with Medicinal

Applications J Med Chem., 56, pp 388-405.

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nicotinic acetylcholine receptor from fetal rat muscle expressed in Xenopus oocytes.

Eur J Pharmacol, 309, p 287

[3] El Nemr A., 2000 Synthetic Methods for the Stereoisomers of Swainsonine and its

Analogues Tetrahedron, 56, p 8579.

[4] Johansson T., Weidolf L., Popp F., Tacke R., Jurva U., 2010 In Vitro Metabolism

of Haloperidol and Sila-Haloperidol: New Metabolic Pathways Resulting from Carbon/Silicon Exchange Drug Metab Dispos, 38, 73.

[5] Lukevics E I S., Germane S., Zablotskaya A., 1997 Silyl modification of

biologically active compounds Chem Heterocycl.Compd., 33, p 234.

[6] Stromberg V L., Horning E C., 1955 Pinus Alkaloids The Alkaloids of P sabiniana

Dougl and Related Species J Am Chem Soc., 77, p 6361.

[7] Tacke R., Handmann V I., Bertermann R., Burschka C., Penka M., Seyfried C., 2003 Sila-Analogues of High-Affinity, Selective σ Ligands of the Spiro[indane-1,4-piperidine] Type: Syntheses, Structures and Pharmacological Properties Organometallics, 22, p 916.

[8] Vivet B., Cavelier, F., Martinez J., 2000 Synthesis of Silaproline, a New Proline

Surrogate Eur J Org Chem., pp 807-811.

[9] Wannagat U., Wiese D., Struckmeier G., Thewalt U., Debaerdemaeker T.,

1988 Sila-Pharmaca, 38 - Structure and Pharmacological Effectiveness of

Sila-Substituted Analogues of the Psychopharmacological Agent Dimetacrine.

Liebigs Ann Chem., pp 241-248

[10] Yingjian Bo, Swapnil Singh, Hoan Quoc Duong, Cui Cao and Scott McN Sieburth,

2011 Efficient, Enantioselective Assembly of Silanediol Protease Inhibitors Org.

Lett., 13 (7), pp 1787-1789

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