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Neurofeedback in children with attention-deficit/ hyperactivity disorder (ADHD) – a controlled multicenter study of a non-pharmacological treatment approach

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Attention-deficit/hyperactivity disorder (ADHD) is the most common neurobehavioral disorder of childhood and has often a chronic course persisting into adulthood.

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S T U D Y P R O T O C O L Open Access

Neurofeedback in children with attention-deficit/

multicenter study of a non-pharmacological

treatment approach

Martin Holtmann1*†, Benjamin Pniewski1†, Daniel Wachtlin2, Sonja Wörz3and Ute Strehl3

Abstract

Background: Attention-deficit/hyperactivity disorder (ADHD) is the most common neurobehavioral disorder of childhood and has often a chronic course persisting into adulthood However, up to 30% of children treated with stimulants either fail to show an improvement or suffer adverse side effects, including decreased appetite, insomnia and irritability and there is no evidence of long term efficacy of stimulants for ADHD A series of studies has shown that neurofeedback is an effective additional or alternative treatment for children with ADHD, leading to e.g

significant and stable improvement in behavior, attention and IQ Significant treatment effects of neurofeedback have also been verified in meta-analyses Most of the trials, however, have been criticized for methodological difficulties, particularly lacking appropriate control conditions and number of patients included This randomized study examines the efficacy of slow cortical potentials (SCP) -neurofeedback, controlling unspecific effects of the setting by comparing two active treatment modalities

Methods/Design: A total of 144 patients with ADHD, older than six and younger than ten years, in some cases with additional pharmacological treatment, are included in this trial In five trial centres patients are treated either with SCP-feedback or electromyographic (EMG) -feedback in 25 sessions within 3 months A comprehensive test battery is conducted before and after treatment and at follow-up 6 month later, to assess core symptoms of ADHD, general psychopathology, attentional performance, comorbid symptoms, intelligence, quality of life and cortical arousal

Discussion: The efficacy of SCP-feedback training for children with ADHD is evaluated in this randomized

controlled study In addition to behavior ratings and psychometric tests neurophysiological parameters serve as dependent variables Further, the choice of EMG-biofeedback as an active control condition is debated

Trials registration: Current Controlled Trials ISRCTN76187185 Registered 5 February 2009

Keywords: Neurofeedback, SCP, Slow cortical potentials, ADHD, Attention-deficit/hyperactivity disorder,

Electromyogram, EMG biofeedback

* Correspondence: martin.holtmann@wkp-lwl.org

†Equal contributors

1

Hospital for Child and Adolescent Psychiatry, LWL University Hospital Hamm

of the Ruhr-University Bochum, Heithofer Allee 64, 59071 Hamm, Germany

Full list of author information is available at the end of the article

© 2014 Holtmann et al.; licensee BioMed Central Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article,

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Attention-deficit/hyperactivity disorder (ADHD) is the

most common neurobehavioral disorder of childhood with

an estimated prevalence of 3-5% in school-aged children

[1,2] Core symptoms include according to DSM-5

im-paired attention, excessive motor activity, and impulsivity

[3] The disorder often has a chronic course with 30-65%

of affected children displaying ADHD symptoms in

adult-hood [4] Numerous problems are associated with ADHD,

including poor social relationships, higher risk taking

be-havior, and a higher incidence of anxiety and depression

symptoms Stimulant medication, e.g methylphenidate,

represents the most commonly used intervention for

chil-dren with ADHD However, up to 30% of chilchil-dren treated

with stimulants either fail to show an improvement or

suffer adverse side effects, including decreased appetite,

insomnia and irritability as well there is no evidence of

long term efficacy of stimulants for ADHD [5,6] After

medication washout children experience considerable

loss of improvement [7] Kratochvil et al [8] reported

on the effectiveness and tolerability of long-term

ato-moxetine treatment During a period of 2 years, more

than 25% of young children with ADHD discontinued

the treatment because of lack of effectiveness

Neurofeedback (NF) as an additional or alternative

treatment is based on neurophysiological changes

char-acteristic of ADHD children [9-11] (for an overview, see

Becker & Holtmann, [12]; Holtmann & Stadler [13])

This intervention has gained promising empirical

sup-port in recent years Before the initial application of this

clinical study, pilot studies had been carried out in three

of the five participating centers (Frankfurt, Göttingen,

Tübingen) For a group of nearly 50 ADHD children,

significant improvement in behavior, attention and IQ

was observed after NF All changes were stable or even

improved at six month [14,15] and two year follow-up

[16] Preliminary results of a prospective randomized

pilot study in 34 ADHD children comparing NF and a

computerized cognitive training indicate that only NF

showed specific effects on impulse control [17] Also,

data of a study in children with ADHD comparing NF

with a computerized cognitive training found an

advan-tage of NF on behavioral and neurophysiological

param-eters (Gevensleben et al., 2007) This supports the data

of Heinrich et al [18] who provided first evidence for

specific neurophysiological effects of NF in children with

ADHD, evidenced in a normalization of the contingent

negative variation (CNV), an event-related correlate of

attentional resources In a controlled functional

mag-netic resonance imaging (fMRI) study, Lévesque et al

[19] demonstrated the capacity of NF to normalize key

neural substrates of selective attention in ADHD

Fur-ther, it has been demonstrated that NF can lead to

microstructural changes in white and gray matter [20]

Several meta-analyses have been published on the ef-fects of neurofeedback on ADHD symptoms A first meta-analysis examined 467 subjects from 10 prospect-ive, controlled neurofeedback trials [21] and showed medium to high effects for all three core domain of ADHD symptoms A second meta-analysis, using a more rigorous methodological approach [22] found a significant treatment effect of neurofeedback (ES = 0.59; 95% CL: 0.31-0.87) using ADHD scores from raters (often unblinded) close to the therapeutic setting Since blinded assessments were only available from four out of the eight included studies, the authors concluded that better evidence for effi-cacy from blinded assessments is required before neuro-feedback can be supported as treatment for ADHD core symptoms This need is addressed in the present study that applies assessments by a blinded clinical investigator In addition, there is still lack of evidence whether the observed effects are specific results of the neurofeedback treatment and are not triggered by unspecific effects

Many studies on neurofeedback in ADHD have been criticized for lacking appropriate controls and follow-up, failing to randomly allocate participants to treatment conditions, using poor diagnostic criteria, and employing subjective and unblinded outcome measures [13,23] In addition, they failed to take into account the influence of the training setting provided by extensive biofeedback These limitations inhibit the acceptance of neurofeedback within the psychiatric and psychological communities Therefore, the aim of this randomized, controlled trial with parallel groups is to examine the efficacy of neuro-feedback in comparison to a self-regulation training using

a peripheral physiological parameter, controlling for pos-sible unspecific positive effects of the training setting First, we hypothesize that neurofeedback will show a bene-ficial effect on the core symptoms of children with ADHD that is superior compared to the control condition Sec-ond, we anticipate that patients will be able to sustain clinical improvement following neurofeedback after having washed out pharmacotherapy Furthermore, we want to investigate the effect of NF on the degree of ill-ness, the attentional performance, comorbid symptoms, intelligence, quality of life and cortical arousal For the first time adverse events as well as severe adverse events will be assessed

Methods

Patricipants and recruitment

Participants are being recruited and treated in the five trial centers (LWL University-Hospital Hamm, Ruhr-University Bochum; Central Institute of Mental Health Mannheim; Institute of Medical Psychology and Behavioral Neurobiol-ogy, Eberhard-Karls-University Tübingen; Department of Child and Adolescent Psychiatry, University of Göttingen; and Department of Child and Adolescent Psychiatry and

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Psychotherapy, Goethe-University Frankfurt) as

outpa-tients from seven to under 10 years with an ADHD

diag-nosis (for all eligibility criteria see Table 1) The diagdiag-nosis

is being verified in a semi structured interview based on

the German adaptation of the Kiddie-Sads-Present and

Lifetime Version (K-SADS-PL [24]) by one of the main

in-vestigators Considering the sample size calculation, 144

subjects are enrolled in the clinical trial (72 subjects per

treatment group) The Interdisciplinary Center for Clinical

Trials (IZKS) of the University of Mainz is responsible

for monitoring the study and for project-, safety-, data

management and biostatistics The IZKS Mainz is

sup-ported by the grant “Clinical Trial Centers [Klinische

Studienzentren], no FK 01KN1103, IZKS Mainz” from

the Federal Ministry of Education and Research

Ethics and written consent

This study (ISRCTN76187185) was approved by all local

Ethics Committees according to the Declaration of

Helsinki Before entering the study patients are informed

about the study objectives, study design, and potential risks

by one of the main investigators and receive this

informa-tion in writing also Written consent is obtained from all

participants and their persons in charge of primary custody

In addition children sign an adequate informed consent

Interventions

Experimental group: feedback of slow cortical potentials

Interventions were conducted following protocols of

pre-vious controlled studies with positive neurofeedback

out-comes [25-28] (for an overview, see Gevensleben et al

[29]; Arns et al [30]]) Slow cortical potentials (SCPs) are

very slow changes in the EEG and belong to the family of

event related potentials They reflect the excitability of the

underlying cortical area While negative shifts mobilize

resources for the preparation of motor and cognitive answers to a stimulus, positive shifts reflect inhibition

of mobilization [15]

Participants randomly allocated to the experimental group receive 25 sessions of neurofeedback of SCPs, with each session lasting about 60 minutes This in-cludes time needed for electrode montage as well as four

10 minutes blocks of feedback Each block consists of 40 trials and each trial is composed of a baseline-phase (2 seconds) and a feedback-phase (8 seconds) The feed-back electrode is being placed at Cz and referenced against an electrode behind the left ear (mastoid) In addition one ground electrode is placed on the right mastoid and four electrodes around the eyes to enable

an online correction of artefacts produced by eye move-ments The training protocol prompts either negative potential or positive potential shifts compared to the baseline [15] If patients are successful for at least 2 sec-onds in total during the second half of the feedback-phase

a sun is shown as a positive reinforcement Negativation and positivation are trained in a randomized succession, within the first 12 training sessions in a 1:1 ratio Respect-ively the focus on negativation, the ratio is being changed

to 4:1 within the last 13 sessions

Participants are sitting in front of a computer screen and connected to a multichannel amplifier which re-cords EEG as well as EMG activity (NEURO PRAX®; neuroConn GmbH, Ilmenau, Germany) Using previous research as a basis the treatment is being administered two to three times a week, and feedback animations pro-vided are simple During each session feedback is given in block 1, 2 and 4 To enable transfer of self-regulation skills

to everyday life participants perform the third block of each session without continuous feedback (transfer trials) Between the 12th session and the 13th session a period of

Table 1 Eligibility criteria

Inclusion criteria Attention-deficit/hyperactivity disorder (combined type) (DSM-IV)

Being 7 to 9 years of age Ability to understand character and individual consequences of the trial Written informed consent of the person with primary custody must be available before enrolment in the trial Exclusion criteria Diagnosis of bipolar disorder, psychosis, serious OCD, chronic serious tics or Tourette syndrome

Major neurological or medical illness Pharmacotherapy for severe anxiety and mood disorders and psychosis Acute suicidal tendencies

IQ below 80 (CPM) Non-German speaking child and primary caretaker

No telephone Pregnancy and lactation Participation in other clinical trials and observation period of competing trials, respectively.

CPM Coloured Progressive Matrices, DSM-IV Diagnostic and Statistical Manual of Mental Disorders, version IV, OCD Obsessive Compulsive Disorder.

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3 weeks without training sessions is being arranged.

During that period participants have to conduct short

transfer exercises where they use the strategies of

self-regulation at least three times a day Little memo-cards

that depict the monitor with a successful self-regulation

trial serve as a cue to remind the participants to

exer-cise the self-regulation In addition they receive a DVD

with a video showing trials with both tasks simulating

the situation in the lab to prompt self-regulation at

home To strengthen the transfer the last 10 sessions

are followed by a short transfer exercise as described

above before doing some homework in the lab

Partici-pants are able to earn a certain amount of tokens for

taking part and good cooperation These tokens are

honored by little presents or vouchers as soon as a

cer-tain amount is collected

Control group: feedback of electromyographic activity of

the musculi supraspinatus

To control the degree of researcher-participant interaction,

as well as other non-specific effects of the feedback-setting,

children assigned to the control group attend 25 sessions of

electromyography (EMG-) biofeedback The training

proto-col reinforces muscular contraction and relaxation of either

the left in relation to the right Musculus supraspinatus or

vice versa, compared to the baseline Two electrodes are

placed at the upper shoulder area (left and right)

Partici-pants of the control group are being instructed to move the

object on the screen by contracting and relaxing these

mus-cles Duration of sessions, amount of transfer trials, surface

of the feedback monitor, reinforcement schedules, electrode

montage and transfer exercises are identical in both groups

Both interventions are being administered in addition to

treatment as usual (TAU) TAU may comprise

pharmaco-therapy with psychostimulants (short or long acting), e.g

methylphenidate and amphetamine salts, and

atomoxe-tine, and medication for Oppositional Defiant Disorder

(ODD) and Conduct Disorder (CD), that are treated

pharmacologically similar to ADHD To assess the unique

effects of the interventions, their stability over time and

the need of further pharmacotherapy, medication is being

washed out before the pretest to establish an unmedicated

baseline and after the intervention phase in both groups

The duration of the washout is adapted to the type of

medication, with a 2-week period for psychostimulants

and 4 weeks for atomoxetine In cases of lacking

practic-ability a minimum washout period of 48 hours (for

psy-chostimulants) and/or 5 days (for atomoxetine) is allowed

and medication is administered again in case of relapse

The timeline of the study is shown in Figure 1

Randomization and blinding

After screening, patients are randomly assigned to either

the neurofeedback training (experimental group) or

electromyographic (EMG)-biofeedback training (control group) Randomization is stratified according to trial site and sex in a 1:1 ratio (neurofeedback vs EMG-biofeedback) Other prognostic factors are considered by means of adjusted analysis A web based randomization tool developed at IZKS Mainz is used within this trial Randomization lists generated at IZKS Mainz by means of

a SAS (Statistical Analysis System) program are imported into this tool For practical reasons, the primary investiga-tors of the study who deliver treatments (but are not in-volved in outcome ratings) are aware of the participants’ allocation, but all medical consultants are blinded to the allocation Also parents and participants are not being in-formed about the randomization outcome but from the start of the training sessions the latter are receiving in-structions dependent on the outcome All participants are connected with both peripheral (EMG) and scalp (EEG) electrodes Training devices and software are identical for both interventions

Assessments FBB-ADHS

The German ADHD Rating scale consists of 20 items that assess the severity and perceived burden of inattention, hyperactivity, and impulsiveness as defined by the ICD-10 and DSM-IV and has been used widely in treatment studies for ADHD [31] It can be used to directly compare effect sizes between different kinds of interventions The German version has shown good psychometric properties, with good internal consistency (α coefficient = 0.78 – 0.89) for parent-rating [32] and (α coefficient > 0.90) for teacher-rating, and good inter-rater reliability (r = 0.70) [33]

Clinical Global Impression Scale

Assessment of general psychopathology is being per-formed using the Clinical Global Impression Scale (CGI)

to estimate symptom severity (CGI-S) and improvement (CGI-I) [34,35] The CGI is a seven-point scale that re-quires a rating of illness severity at the time of assessment Because severity estimation in the CGI is performed in re-lation to other patients, it is a subjective assessment tool Ratings are performed by a medical consultant blinded to group allocation

Testbattery for Attentional Performance

The Testbattery for Attentional Performance (TAP) ex-amines a large spectrum of specific attentional perfor-mances in a computerized form [36] The following two subtests are being administered

1) Go/Nogo This subtest assesses the ability to suppress a response in the presence of irrelevant stimuli Omission errors, committed errors, average reaction time and intraindividual variance of

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reaction time are analyzed in both subtests to

estimate changes in the attention performance

2) Flexibility (non-verbal) This subtest assesses the ability

to shift the attentional focus, measuring reaction times

for valid and invalid cues in a visual task

The TAP is a well validated measurement with

satisfy-ing psychometric properties [36]

Neurophysiological parameters: a) quantitative EEG and

b) event related potentials

Two neurophysiological parameters are ascertained using

an EEG amplifier (19 electrodes, 10–20 system):

a) Quantitative EEG (QEEG) is recorded during

6 minutes resting condition (3 minutes eyes closed

and 3 minutes eyes open) and during the first, last

and follow-up training session, with the same

equipment used for feedback but at 19 electrodes

After correction for eye artefacts from the recording

channels power spectral analysis, displayed as a

topographical brainmap showing the absolute and

relative power for delta, theta, alpha and beta

frequency are analyzed

b) Event related potentials (ERPs) are recorded in a

cued Continuous Performance Task (CPT-OX [37])

The CPT-OX measures a person's sustained and selective attention and impulsivity By its embedded Go/Nogo task the CPT-OX captures a sequence of attentional and preparatory brain processes initiated

by the cue stimulus (indexed by Cue P300 and CNV) which precede inhibitory control (indexed by Nogo P300 (e.g [38]) The average reaction time, number of omission and commissions and mean amplitude of CNV and P300 is assessed Additionally Error-related negativity (ERN, a marker of action monitoring and error processing) is recorded in an Erikson flanker task (ERN-FT [39]) Average reaction time and number of errors for each condition (congruent/incongruent) is assessed

Child Behavior Checklist

The Child Behavior Checklist (CBCL) is one of the best-studied, empirically derived parent checklists for meas-uring general child and adolescent psychopathology [40] and is applied to assess comorbid symptoms The child’s behavior over the past 6 month using a total of

118 items (plus 2 optional questions) is rated by parents

or primary caregivers The questionnaire includes a total problem score, two superior scales (externalizing problems and internalizing problems), and eight syndrome scales (withdrawal, somatic complaints, anxiousness/depression,

Screening:

Diagnosis ADHD

Check inclusion criteria

Informed consent

4 weeks Medication washout 5 months

6 months Follow-up

3 months Treatment-phase Screening phase

Neurofeedback

25 sessions within 3 months under medication

EMG-Feedback

25 sessions within 3 months under medication

Post-Tests 2 minus Pretests

Main outcome: Specific Effects of Neurofeedback?

Follow-up Tests minus Post-Tests 2

Sustainability of effects?

2 - 4 weeks Medication washout

Figure 1 Flow chart.

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social problems, thought problems, attention problems,

delinquent behavior, and aggressive behavior) The

reli-ability, factorial validity and discriminant validity of the

German adaptation of the CBCL have been confirmed

by several studies [41,42] In case of comorbid

symp-toms (assessed by the individual syndrome scores)

add-itional corresponding parents’ rating scales of the

Diagnostic-System for Mental Disorders in Children

and Adolescents (DISYPS-KP [43]) are applied

Strengths and Difficulties Questionnaire

In addition to the CBCL, the Strengths and Difficulties

Questionnaire (SDQ) is used in order to assess

short-term changes of comorbid symptoms and evaluate

par-ent and teacher ratings The SDQ is a brief behavioral

screening questionnaire assessing 25 attributes, some

posi-tive and others negaposi-tive, which can be allocated to five

scales (emotional symptoms, conduct problems,

hyper-activity/inattention, peer relationship problems, and

pro-social behavior) [44] These scales can be summed to

calculate a total difficulties score with the advantage of

be-ing able to assess short-term changes [45] In this study

parent and teacher ratings with the SDQ are used The

sensitivity to change of SDQ allows estimation of

treat-ment efficacy (α = 0.73; retest stability = 0.62) [46] and

monitoring of symptom changes compared to screening

Coloured Progressive Matrices

Full scale intelligence quotient (IQ) is measured using the

Coloured Progressive Matrices (CPM) [47] The CPM is a

language-free intelligence screening test consisting of 36

items and standardized for children between 4 and 11 years

of age It provides a parallel version (Split-Half Reliability

of r = 0.85 to 0.90) to minimize test-retest-effects

(test-re-test coefficient r = 0.86 to 0.90)

Quality of life questionnaire

The revised German Kid-KINDL(R) quality of life 24 item

questionnaire is a reliable, valid and practicable

instru-ment [48] yielding six dimensions (body, psyche,

self-esteem, family, friends, and functional aspects) and a total

score The self-rating for children between 7 and 13 years

of age is applied to participants The internal consistency

for subscales reached values fromα = 0.54 to α = 0.73, with

anα = 0.82 for the total score [49]

Parents’ expectations on-/satisfaction with therapy

The expectations before treatment, and the satisfaction

with therapy during and after treatment is rated by parents

using a questionnaire that was developed by the Institute

of Medical Psychology and Behavioral Neurobiology,

Tübingen [50] The questionnaire consists of six questions

based on a 6-point Likert scale To avoid bias through

social expectancy parents submit the questionnaire dir-ectly to IZKS Mainz

Adverse events and serious adverse events

At each contact (assessment and training-session) partic-ipants are asked to report any adverse events (AEs) AEs are assessed by using open questions, asking about gen-eral AEs and their severity during the study period

Time points of assessments

The assessment time points are as follows

 Screening: Eligibility criteria are examined in a first appointment

 Pre-test: After washing out the medication as outlined above the initial assessment is carried out

to establish an unmedicated baseline

 Post-test I: After 3 months of either neurofeedback

or EMG-biofeedback, there is a comprehensive post-therapy assessment

 Post-test II: One month later, a second comprehensive post-therapy assessment is provided

To compare an unmedicated state to the baseline (established in the Pre-test) again medication is washed out before

 Follow-up-test: Six months after the training-phase has ended, another comprehensive assessment is carried out Medication is washed out before

Additionally the parent version of the FBB-ADHS is assessed monthly An overview of the time points and the applied assessments is given in Table 2 All ques-tionnaires are presented in German, and are being com-pleted within the different treatment centers with exception of the teachers’ assessments (surface mail) and the monthly assessment of the FBB-ADHS after Post-test II (phone)

Primary and secondary endpoints

The primary endpoint of this study is the change in ADHD rating scale (FBB-ADHS) after treatment and washout of medication (Post-test II minus Pretest) Secondary endpoints include:

 CGI-I

 Resumption of medication by choice of family during follow-up

 Change in neuropsychological and neurophysiological parameters

 Change in SDQ questionnaire subscales

 Change of full-scale IQ in CMP

 Change in KINDL(R) questionnaire (both parents and child version)

 Score measuring parents' satisfaction with therapy

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Pre-specification

A detailed methodology for summary and statistical

ana-lysis of the data collected in this trial is documented in a

Statistical Analysis Plan (SAP) that is dated and

main-tained by IZKS Mainz The document may modify the

plans outlined in the study protocol; however any major

modifications of the primary endpoint definition and/or

its analysis are also reflected in a protocol amendment

The SAP has to be authorized before database closure

by the biometrician and the coordinating investigator

Sample-size calculation

Estimates of a clinically relevant effect size were derived

from the Göttingen pilot-study using the same primary

outcome measures [18] It is expected that in the

neuro-feedback group the mean FBB-ADHS score at Post-Test

2 is 1.20 and in the control group 1.50 with a common

standard deviation of 0.55 The expected outcome

re-quires a sample size of 72 subjects per group (α = 0.05,

two sample t-test, two-sided) to achieve a power of 90%

Statistical analysis

Data are analyzed primarily in the modified

intention-to-treat (mITT) population Supportive analyses are planned

in the per-protocol (PP) population mITT comprises all

randomized patients with the exception of patients for

which it is obvious at the time of randomization that no

study specific therapy would be applied, while PP analysis assesses mITT patients who do not meet any of the fol-lowing criteria: violation of inclusion and/or exclusion cri-teria, major deviations from the visit schedule, and bad compliance during feedback sessions All safety parame-ters are analyzed in the safety population comprising all patients participating in at least one feedback session Within these analyses patients are analyzed according to the received treatment even if the respective patient was randomized to the other treatment group

In the primary analysis the primary outcome is tested by

an analysis of covariance (ANCOVA) using treatment, trial site, baseline FBB-ADHS score, baseline ADHD medica-tion, parenting style, parent’s expectations, and sex as co-variates The analysis is repeated for the PP population as

a sensitivity analysis In further analyses other potential predictors of response to treatment are examined Second-ary outcome measures comprise binSecond-ary variables and scores derived from standardized questionnaires For bin-ary variables proportions and relative risks together with their associated 95% confidence-intervals are calculated Differences between intervention groups are assessed using logistic regression models Scores are described

by distributional parameters (mean, standard deviation, median, quartiles, and range) In case the assumption of normally distributed data can not be rejected, differ-ences in the scores between intervention groups are an-alyzed by an ANCOVA using the same predictors as in

Table 2 Time points of assessments

Phase

Follow-up-test Action

Parents ’ expectations/

satisfaction

CBCL Child Behavior Checklist, CGI Clinical Global Impression Scale-Severity /Improvement, CPM Coloured Progressive Matrices /parallel version, EFB-K Erziehungsfragebogen (German version of the PS, Parenting Scale), ERPs Event related potentials, FBB-ADHS Fremdbeurteilungsbogen für Aufmerksamkeitsdefizit-/Hyperaktivitätsstörungen (German ADHD rating scale), Kid-KINDL(R) Revised German quality of life questionnaire, QEEG Quantitative EEG, SDQ Strengths and Difficulties Questionnaire, TAP Testbattery for Attentional Performance.

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the primary analysis In case of major deviations from

ANCOVA requirements, appropriate non-parametric

methods are applied

Quality assurance

Data management

A detailed methodology for the data management in this

trial is documented in a data management plan that is

dated and maintained by IZKS Mainz This plan is signed

by the sponsor, the head of the data management team and

the responsible data manager This trial is performed using

an electronic case report form (eCRF) or remote data entry

(RDE) The investigator and the trial site staff receive

sys-tem documentation, training and support for the use of the

eCRF During data entry integrity checks help to minimize

entry failures These data entry checks are based on the

data validation plan Any missing data or inconsistencies

are reported back to the respective site and clarified by the

responsible investigator After completion of data entry and

if no further corrections are to be made in the database, the

access rights are taken away and the database is declared

closed and used for statistical analysis

Monitoring

Monitoring is done by personal visits from a clinical

moni-tor according to prior defined standard operation

proce-dures of the IZKS Mainz By frequent communications

(letters, telephone, fax), the site monitor ensures that the

study is conducted according to the protocol and regulatory

requirements The investigator has to allow the monitor to

look at all essential documents and has to provide support

at all times to the monitor Furthermore, queries are

re-solved in cooperation with the investigator Close-out visits

are conducted to close the study site at the end of the study

and to ensure that all study-related documents archived

Advisory board

An independent advisory board is established This

ad-visory board is supposed to act as a data monitoring and

safety board during non-public meetings in the absence of

the principal investigator The advisory board supervises

the conduct of the study and issues recommendations for

early termination, modifications or continuation of the

study, if necessary It has to be informed contemporary of

serious study related events

Discussion

This paper presents the design and protocol of a

random-ized controlled trial (RCT) with neurofeedback for

chil-dren with ADHD in an outpatient setting The choice of

EMG biofeedback as a control condition was made after

an extensive discussion Although there is no doubt

that double-blind, placebo-controlled trials could provide

strong evidence for the efficacy and specificity of a given

treatment, there are several issues of a“sham” condition specially in neurofeedback Apart from the ethical issues, the feasibility of a sham condition for neurofeedback is doubtful Birbaumer et al [51] once tried to establish a double-blind sham condition in a neurofeedback trial for patients with epilepsy Patients as well as the trainer “de-tected” the sham sessions and refused further cooperation Furthermore neurofeedback in particular seems to induce the assumption that one is part of the placebo control In previous placebo-controlled trials of neurofeedback, up to 80% of the participants of the neurofeedback groups esti-mated (after treatment) that they received placebo feed-back [52,53] As we have learned from one of our pilot studies [15] it takes time until children are able to self-regulate their brain activity Therefore clinical improve-ment becomes evident only after a certain delay The impression of uncontrollability that arises especially in the beginning might assume that missing effects are due to the control condition and therefore may lead patients to discontinue participation Therefore, the present design made use of EMG biofeedback as an alternative control condition, based on the following considerations: There are not many studies on EMG Feedback available with satisfy-ing methods and/or results A review of 44 studies [54] concludes that the data do not suggest that biofeedback (i.e EMG) techniques are superior to more conventional treat-ment A detailed analysis of the studies included in this re-view leads to an even more pessimistic estimation of the effects Original work published after 1981 does not show much improvement No study reports follow-up data (e.g [55]), diagnosis is based on teachers’ ratings [56], some of the improvements are found in the placebo-group as well [57] and EMG Feedback was frequently carried out in addition to other treatments Arnold [58] concludes from his comprehensive review that EMG Feedback“merits fur-ther studies” Although not tightly connected to the known pathology of ADHD, relaxation of muscles can have effects

on the EEG [59] Therefore possible changes in the EEG are controlled by the QEEG (secondary measure) in this study As shown by Bakhshayesh et al [60] EMG Feedback does have an effect on core symptoms of ADHD albeit not

as much as EEG-Feedback Therefore from an ethical view-point EMG-Feedback is not just “empty” and senseless, possibly reflecting the desirable placebo effect of the treat-ment as being rewarded for being attentive, concentrated and cooperative Moreover it is to mention that a blinded setting might work well in drug research but seems an in-appropriate requirement in psychotherapeutic treatments where subjects are supposed to learn a certain behavior In Biofeedback the task is to acquire a self-regulation skill At least the first stage of this learning process requires con-scious control over the target variable [61] As observed by Surwit and Keefe [62] without knowing which parameter is being trained subjects are less effective in the acquisition of

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control This all amounts to the conclusion that the EMG

biofeedback applied in this design is an adequate, satisfying

and credible control condition If the trial supports the

ef-fectiveness of neurofeedback in reducing ADHD core

symptoms in the absence of stimulant therapy, this would

offer an effective alternative for those ADHD patients

whose treatment is up to now limited by poor medication

response, adverse side effects, and in cases in which the

pa-tients and/or their parents refuse medication treatment In

children who respond to pharmacotherapy, medication

could be withdrawn after successful neurofeedback training

Beyond the immediate research setting, neurofeedback

might be expanded to other behavioral disorders

The present study examines the effects of neurofeedback

on behavioral as well as neurophysiological parameters

comprising attentional, preparatory, time processing, and

inhibitory ERP components In a longitudinal study of

neuropsychological and electrophysiological markers of

different ERP components Doehnert et al [63] suggest

that especially preparatory and time processing brain

pro-cesses indexed by CNV remained detectable in young

adult ADHD subjects, even in ADHD remitters, compared

to controls The results seem to indicate residual timing

deficits even in young adults with remitted ADHD

Hein-rich et al [18] investigated the effects of a neurofeedback

training aiming at generating a ‘more negative’ CNV in

children with ADHD compared with a waiting-list group

Besides a reduction of ADHD symptoms following the

training, a pronounced increase of the CNV amplitude

was observed in CPT cue trials The authors suggested

that the CNV increase may be interpreted as a

neuro-physiological correlate of improved self-regulatory

capabil-ities Expecting a relevant stimulus, children with ADHD

may be able to allocate more resources after neurofeedback

Interestingly, similar effects on the CNV were reported

fol-lowing cognitive-behavioral interventions Against that

background future neurofeedback studies may directly aim

at the modulation of CNV and explicitly address impaired

preparatory processes and timing as an important target of

treatment [64]

Competing interests

This work is funded by the German Research Foundation (DFG; ref: HO 2503/

4-1; HO 2503/4-2).

MH has served in an advisory or consultancy role for: Lilly, Novartis, and

Bristol-Myers Squibb, and has received conference attendance support or

was paid for public speaking by AstraZeneca, Janssen-Cilag, Lilly, Neuroconn,

Novartis, Medice, and Shire BP was paid for public speaking by Lilly and

Novartis US was paid for public speaking by Novartis, Medice, Neuroconn,

the German Society for Biofeedback and Akademie König und Müller The

present work is unrelated to the above grants and relationships The other

authors have no conflicts of interest.

Authors ’ contributions

MH and US conceived the research project; they designed the study; and

together with SW and DW designed and tailored the study protocol All

authors contributed to the writing of the manuscript All authors read and

approved the final manuscript.

Authors ’ information Ute Strehl Senior author.

Author details

1 Hospital for Child and Adolescent Psychiatry, LWL University Hospital Hamm

of the Ruhr-University Bochum, Heithofer Allee 64, 59071 Hamm, Germany.

2 Interdisciplinary Centre for Clinical Trials (German abb.: IZKS), University Medical Centre Mainz, Langenbeckstr 255131 Mainz, Germany.3Institute of Medical Psychology and Behavioral Neurobiology, Eberhard-Karls-University Tübingen, Silcherstr 5, 72076 Tübingen, Germany.

Received: 26 June 2014 Accepted: 7 August 2014 Published: 13 August 2014

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