Objectives: To determine some histopathological features and evaluate the expression of some IHC markers in prostate carcinoma (Pca). Subjects and methods: Histopathological study was performed on 84 specimens by using hematoxylin and eosin (H.E) stained tissue sections. IHC study was performed on 33 specimens by using IHC stained tissue sections with PSA, CK34βE12, p63, CK7, CK5/6, actin monoclonal antibodies.
Trang 1STUDY ON SOME HISTOPATHOLOGICAL FEATURES AND
EXPRESSION OF SOME IMMUNOHISTOCHEMICAL MARKERS
IN PROSTATIC CARCINOMA
Vi Thuat Thang*; Tran Ngoc Dung*; Nguyen Dinh Tao**
Nguyen Ngoc Hung*; Tran Ngoc Anh**
SUMMARY
Objectives: To determine some histopathological features and evaluate the expression of some
IHC markers in prostate carcinoma (Pca) Subjects and methods: Histopathological study was
performed on 84 specimens by using hematoxylin and eosin (H.E) stained tissue sections IHC
study was performed on 33 specimens by using IHC stained tissue sections with PSA, CK34βE12,
p63, CK7, CK5/6, actin monoclonal antibodies Results: Prostatic adenocarcinoma accounted for
96.4% (acinar type was the most common) and urothelial carcinoma of the prostate accounted for
3.6% Gleason scores 5 - 7 were the most common Of the 84 patients, 60 (73.2%) had high grade
prostatic intraepithelial neoplasia (HGPIN) associated adenocarcinoma There were 39% of
adenocarcinoma with perineural invasion and 53.7% of adenocarcinoma with mucinous secretions
IHC showed 31 cases (94%) of Pca which were positive for PSA and 2 cases (6%) of urothelial
carcinoma of the prostate were negative for PSA The lower Gleason grade was stronger positive for
PSA was and vice versa All the tumor cells were negative for 34βE12, p63, CK7, CK5/6 and actin
Conclusion: Our data indicate that most of Pca are prostatic adenocarcinoma and Gleason scores
5 - 7 are the most common scores HGPIN is strongly associated with adenocarcinoma Other
features are commonly seen in prostatic adenocarcinoma which are blue-tinged mucinous
secretions and perineural invasion IHC shows all of prostatic adenocarcinomas are positive for
PSA, urothelial carcinoma of the prostate is negative for PSA The staining intensity of the tumor
cells for PSA is stronger in the lower grade adenocarcinomas and vice versa
* Keywords: Adenocarcinoma of the prostate; Immunohistochemistry
INTRODUCTION
Prostatic carcinoma is one of the
malignant tumours and often found in
men over the age of 65 Nowadays, this
kind of tumour has the tendency to increase
dramatically in almost every country
around the world Histopathologically,
adenocarcinomas of the prostate range
from well-differentiated gland forming
cancers, where it is often difficult to
distinguish them from benign prostatic
glands, to poorly differentiate tumours,
difficult to identify as being of prostatic origin [8] Currently in about two-thirds of these difficult cases, an appropriate diagnosis can be made due to IHC stains with the use of antibodies [4] In Vietnam, the studies on histopathological features and the expression of IHC markers in carcinoma of the prostate are not adequate
to the demand [1] The aim of this study is:
To determine some histopathological features and evaluate the expression of some IHC markers in Pca.
* 103 Military Hospital
** Vietnam Military Medical University
Corresponding author: Vi Thuat Thang (vithuatthang@yahoo.com)
Date received: 23/03/2017
Date accepted: 27/09/2017
Trang 2SUBJECTS AND METHODS
1 Subjects
Eighty four consecutive prostate glands
operated by using transurethral resection
of the prostate (TURP) at Urology of
103 Military Hospital from June, 2008 to
July, 2017
2 Methods
Specimens were fixed in 10% neutral
buffered formalin, embeded in paraffin
and cut on the microtoma Tissue
sections were stained with H.E and IHC
stained with the use of PSA, CK5/6,
CK34βE12, p63, CK7, actin monoclonal
antibodies The results were presented
and discussed
* Distribution the age:
Ages of patients were divided into
5 groups with the gap of 10 years
(< 50, 50 - 59, 60 - 69, 70 - 79 and ≥ 80)
* Study on histopathology:
- Determine the types of histopathology
of the Pca according to the 2004 WHO
histological classification of tumours of the
prostate
- Evaluate the association between
adenocarcinoma and HGPIN
- Grade the adenocarcinomas according
to the Gleason grading system
- Group Gleason scores into diffentiation
categories
- Determine some histopathological
features of prostatic adenocarcinoma
* Study on the expression of some IHC
markers in prostatic carcinoma:
Tissue sections of 33 cases were IHC stained with use of PSA, CK5/6, CK34βE12, p63 and actin monoclonal antibodies, in which there were 31 cases of adenocarcinoma and 2 cases of urothelial carcinoma of the prostate
- Determine the ratio of prostatic
carcinomas which were positive for PSA,
CK34βE12, p63, CK7, CK5/6 and actin
- Distribute staining intensity of tumor cells for PSA according to Gleason grade
RESULTS AND DISCUSSION
1 Distribution of patients’ age
Table 1: Distributed results of Pca among age group (n = 84)
(n = 84)
Ratio (%)
This table showed the incidence of Pca increased with age group The 70 - 79 age group was the highest with 42.86%, the 40 - 49 age group was the lowest with 1.19% These results showed that prostatic carcinoma was rarely found in men below the age of 50 [1, 6, 8] Our results of distributed Pca among age group are the same as the outcome of study carried out by Nguyen Van Hung [1], Nguyen Buu Trieu [2] and others [7]
Trang 32 Study on histopathology
Table 2: Determination of types of
histopathology of the Pca according to the
2004 WHO histological classification
(n = 84)
patients
Ratio (%)
Adenocarcinoma
Acinar adenocarcinoma
Dductal adenocarcinoma
Urothelial carcinoma
Squamous carcinoma
Basal cell carcinoma
82
80
2
2
0
0
96.4 97.6 2.4 3.6
This table showed adenocarcinoma
accounted for 96.4% (in which acinar
adenocarcinoma was 97.6%, ductal
adenocarcinoma was 2.4%) and urothelial
carcinoma of the prostate accounted for
3.6% These results were the same as the
results found by Nguyen Van Hung [1]
and others
Table 3: Association between
adenocarcinoma and HGPIN (n = 82)
patients
Ratio (%)
Adenocarcinoma is
associated with HGPIN
Adenocarcinoma is not
associated with HGPIN
60
22
73.2 26.8
Adenocarcinoma associated with
HGPIN and accounted for 73.2%,
much higher than adenocarcinoma,
did not associate with HGPIN (26.8%)
Nguyen Van Hung [1] also found that
76.2% of prostatic carcinoma contained
HGPIN Clinical significance of HGPIN
showed strong association with Pca [5]
HGPIN was a predictive factor which was valuable in the diagnosis of adenocarcinoma
of the prostate, especially HGPIN with adjacent atypical glands (PINATYP) [8] HGPIN in needle biopsy tissue was a risky factor for the subsequent carcinoma detection after re-biopsy [1, 2, 5, 9]
Table 3: Grouping Gleason score into
diffentiation categories (n = 82)
Differentiated grade
Gleason score
No of patients
Ratio (%)
Well differentiated Moderately differentiated Poorly differentiated
2 - 4
5 - 7
8 - 10
14
58
10
17.1 70.7 12.2
Two cases of urothelial carcinoma of the prostate were not included in this scoring system Gleason score tumors
5 - 7 were the highest ratio (70.7%), Gleason score tumors 2 - 4 and 8 - 10 were 17.1% and 12.2%, respectively These results were the same as the study carried out by Nguyen Van Hung [1], Bostwick, Oesterling, Zincke [1, 3, 5, 10]
Table 5: Distributed ratio of adenocarcinomas
according to malignant specific features (n = 82)
Malignant specific
Ratio (%)
2 or 3 of malignant specific features
No malignant specific features
Trang 4The table showed adenocarcinoma
with perineural invasion was found in
39% of cases, adenocarcinoma with
glorumeration and mucinous fibroplasia
were found in 12.2% and 11%, respectively
Adenocarcinoma with 2 or 3 malignant
specific features were found in 7.3% of
cases while adenocarcinoma with no
malignant specific features were found in
30.5% of cases There were only 3
features that are in and of themselves
diagnostic of cancer These were perineural
invasion, mucinous fibroplasia and
glorumeration Perineural invasion
specimens by prostate cancers were seen
in radical prostatectomy specimens in
75 - 84% of cases Most studies indicate
that its pesesence corellated with
extraprostatic extension (38 - 93%)
Recent data suggest that this finding
may indipendently predict lymph-node
metastasis and prognosis after surgery
[8]
Table 6: Distributed ratio of adenocarcinoma
according to intraluminal features (n = 82)
Blue-tinged mucinous
secretions
Crystalloids and
mucinous secretions
No crystalloids and
mucinous secretions
Crystalloids were found in 9.7% of cases,
blue-tinged mucinous secretions (53.7%),
crystalloids and mucinous secretions (22%),
no crystalloids and mucinous secretions (14.6%)
Crystalloids, despite not diagnostic of carcinoma, were more frequently found in low grade cancer than in benign glands [11] Young [9] also showed that the incidence of crystalloids in the adenocarcinoma ranged 10 - 62% Blue-tinged mucinous secretions seen in H.E stained sections were additional finding seen preferentially
in cancer, especially low grade cancer [8]
Fig.1: Well- differentiated
adenocarcinoma, Gleason grade 2
(Arrow: mucinous secretions) (No S2835 x 200.HE)
Fig.2: Poorly differentiated adenocarcinoma, Gleason grade 4
(Arrow: perineural invasion) (No.S3475 x 400.HE).
Trang 53 Study on immunohistochemistry
Table 7: Ratio of Pca which were positive for PSA, 34βE12, p63, CK7, CK5/6 and actin (n = 33)
PSA
34βE12
p63
CK7
CK5/6
Actin
31/33 0/33 0/33 0/33 0/33 0/33
94%
0%
0%
0%
0%
0%
The study results were consistent with the findings of other studies [1, 4, 8]
Table 8: Staining intensity distribution of tumor cells with PSA according to Gleason
grade (n = 31)
(Positive: +; Faint: 1+; Moderate: 2+; Strong: 3+; Very strong: 4+)
IHC showed tumor cells which were strong positive for PSA in the areas of Gleason
grade 2, moderate positive for PSA in the areas of Gleason grade 3, faint positive for PSA in the areas of Gleason grade 4 and 5 The study results were consistent with the findings of other studies The finding is that the more the differentiated tumor cell is, the stronger the positive intensity for PSA is and vice versa [1, 4, 8]
Fig 3: Gleason grade 2
(Arrow: strong positive)
No N3351 x200 PSA
Fig 4: Gleason grade 3
(Arrow: moderate positive)
No N3351 x200 PSA
Fig 5: Gleason grade 4 (Arrow: basal cells (2+) for 34βE12 in benign glands) (No N2519 x200 34βE12)
Trang 6CONCLUSION
* Histopathological features:
Prostate carcinoma is common in the
older men Most of Pca are adenocarcinoma
in which acinar adenocarcinoma type is the
most common, ductal adenocacinoma is a
rare variant of prostatic adenocarcinoma
Gleason scores 5 - 7 are the most common
Adenocarcinoma is strongly associated
with HGPIN Other features commonly
seen in prostatic adenocarcinoma which
are blue-tinged mucinous secretion and
perineural invasion
* Expression of some immunohistochemical
markers:
IHC shows all of prostatic adenocarcinomas
are positive for PSA, urothelial carcinomas
of the prostate are negative for PSA The
staining intensity of the tumor cells for
PSA are stronger in the lower grade
adenocarcinomas and vice versa Tumor
cells are negative for CK34βE12, p63,
CK7, CK5/6 and actin
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