Objectives: To estimate the result of non-specific detoxification in the people exposed to agent orange/dioxin. Subjects and methods: The study included 34 people (23 men, 11 women) who live around the area contaminated with agent orange/dioxin in Danang were found 2.3.7.8-TCDD in blood in the previous studies.
Trang 1RESULTS OF NON-SPECIFIC DETOXIFICATION IN PEOPLE
EXPOSED TO AGENT ORANGE/DIOXIN
Luong Minh Tuan*; Nguyen Hoang Thanh**
Le Ke Son***; Nguyen Dang Dung****
SUMMARY
Objectives: To estimate the result of non-specific detoxification in the people exposed to agent orange/dioxin Subjects and methods: The study included 34 people (23 men, 11 women) who live around the area contaminated with agent orange/dioxin in Danang were found 2.3.7.8-TCDD
in blood in the previous studies Before and after applying the non-specific treatment using the Hubbard method principles, full blood samples were taken and analyzed the dioxin concentration by the gas chromatography high resolusion mass spectrometry in Eurofins
Analysing Center Cell immune index CD3, CD4, CD8 were analyzed by the Hematology
Department, Military Hospital 103 Humoral immune index IgA, IgG, IgM were analyzed by the Military Medical - Pharmacertical Reseach Center, Military Medical University Results: Before treatment, mean dioxin level in blood of the exposed group was 50.47 ± 130.74 pg/g lipid; after the treatment, this indicator decreased down to 39.29 ± 99.93 pg/g; the difference was significant (p < 0.05) The 2.3.7.8-TCDD concentrations and TEQs clearly showed the reduction trend Concentrations of IgA, IgG and IgM increased significantly: IgA from 281.37 ± 188.74 to 303.86 ± 185.11; IgG from 1103.8 ± 223.5 to 1259.1 ± 286.01; IgM from 121.29 ± 71.35 to 172.59 ± 70.76 Cell immune index: CD3 from 1341.4 ± 454.64 cell/µL to 172.59 ± 70.76 cell/µL; CD4 from 766.59 ± 279.43 cell/µL to 969.85 ± 341.93 cell/µL; CD8 from 443.53 ± 194.22 cell/µL to 555.06 ± 178.39 cell/µL Conclusions: The Hubbard detoxification method can eliminate dioxins out of the body and improve some immune indexes of people who exposed to agent orange/dioxin./
* Keywords: Dioxins; Detoxification; Immune index
INTRODUCTION
From 1961 to 1971, the US Army sprayed
about 80 million litrers of herbicide in the
South of Vietnam (there were 48.6 litres
of agent orange among them - a 50/50
mixture of 2.4-diclorophenoxyacetic acid
and 2.4.5-triclorophenoxyacetic acid, with contaminated dioxin containing TCDD at high rate)
Up to now, there is no specific treatment for the people exposed to agent orange/dioxin Major methods are to inhibit and cure the disorders of the body due to dioxins, including
* Military Instiute of Preventive Medicine
** 103 Military Hospital
*** Ministry of Natural Resources and Environment
**** Vietnam Military Medical University
Corresponding author: Luong Minh Tuan (luongtuan27@gmail.com)
Date received: 10/08/2017
Date accepted: 18/09/2017
Trang 2strengthening the immune system,
anti-oxidant, protection of liver, natural enzyme
therapy, multi peptide, vitamin therapy and
physiotherapy [6] All of these treatments
are non-specific, helping to improve the
general constitution for the patients
No research on detoxification associated
with analysis on dioxins concentration has
been conducted before, during and after
treatment
Non-specific treatment based on the
Hubbard method principles was created
to promote the process of elimination of
xenobiotic in fat tissue, so that the toxic
pollutants in the body decrease In fact,
this method can also eliminate other
contaminated compound It was applied in
many cases over the world and achieved
good effect However, we carried out this
study with a view to: Evaluating the results
of non-specific detoxification in the people
exposed to agent orange/dioxin
SUBJECTS AND METHODS
34 people (23 men, 11 women) living
around Danang air base, which was
contaminated with agent orange/dioxin
have 2.3.7.8-TCDD in their blood according
to the previous studies Study period was
from September to November, 2012 at
103 Hospital, Ha Noi, Vietnam
Non-specific detoxification contains the
following elements:
- Doing gymnastic running is applied to
strengthen blood circulation, especially
peripheral circulation
- Steam bath: combined with taking vitamins and other nutritions in a designed process, especially olive oil, mineral and vitamin PP
- Nutrition: ordinary regime with some kinds of fresh vegetable: extra water consumption to redeem sweating
In addition, the participants slept enough
as prescription The period of detoxification was from 2 to 3 weeks, depending on personal “finishing situation” (according to the requirement of the program)
Doing gymnastic in the program plays
an important role It helps strengthening blood circulation to thrust out the toxic chemical which remained in blood Without gymnastic, detoxification effect will be poor Olive oil, vitamins help to dilute and to eliminate dioxins from tissues especially lipid into circulation and excrete them out Vitamin PP (niacin) promote excreting cholesterol so dioxins can be eliminated with bile, follow the fecal passage [4] Before and after applying the non-specific treatment followed by the Hubbard method principle, full blood sample were taken for analysis of dioxins concentration by the gas chromatography high resolution mass spectrometry (GC/HR-MS) at Eurofins Analysing Center (Germany)
Hematology and biochemistry test were done at 103 Hospital
The data was analyzed by a medical statistic software (Wilcoxon - signed rank test and t-student test - SPSS16)
Trang 3RESULTS AND DISCUSSION
Table 1: Distribution of age, sex of study subject
Age
p > 0.05
Average age of participants ranged from 44.5 ± 13.04 The age group 41 - 60 took the highest rate (50%) and from 61 to 80 took the lowest rate (11.8%) The age and age distribution were not different between male and female groups (p > 0.05)
Some people of the subject group were born after the war in the Southern part of Vietnam (20 - 40 years old) and still infiltrated by dioxins Because of their half-life spent in contaminated areas and exposure with dioxins (about 7 - 11 years), it can be explained why these people have had dioxins in their body [1, 2, 7, 8] According to Nguyen Hoang Thanh (2010), in the study on 26 veterans who took part in the battles
in the Southern part of Vietnam and who now live in uncontaminated sites, nobody has 2.3.7.8-TCDD in their blood although they used to be victims and had 2.3.7.8-TCDD in their body before This results were consistent with remarks by many scientists that 2.3.7.8-TCDD in Vietnam had only one source from agent orange sprayed during the Ranch Hand campaign [2]
Table 2: Results of biochemistry tests before and after detoxification
Subject of the study (n = 34) Indexes
Trang 4Before and after applying the Hubbard detoxification method, only cholesterol index decreased (p < 0.05) Others showed no change This may be due to the effect of niacin (its other name is vitamin B3 or vitamin PP), which plays a vital role, present in more than 200 reactions of the body to release power and delivery matters to build cells Niacin produces 2 coenzymes: nicotinamid adenin dinucleotid (NAD) and nicotinamid adenin dinucleotid phosphate (NADP) with biotin (vitamin H) takes part in analysis of food to valuable active ingredients for the body They help to decrease bad cholesterol (LDL) and increase good cholesterol (HDL)
Table 3: Alteration of hematological indexes after detoxification
Subject of the study (n = 34) Indexes
p (1-2)
Hematological indexes of study group had no change (p > 0.05) Applying Hubbard method did not make any changes to blood cell in our study
Table 4: Alteration of dioxin concentration after detoxification (pg/g lipid)
Study subject (n = 34) Indexes
p (1-2)
Before applying the Hubbard detoxification, the mean concentration of 2.3.7.8-TCDD (pg/g lipid) of exposed group was 50.47 ± 130.74; after the treatment, this index decreased down to 39.29 ± 99.93 The difference was significant (p < 0.05) Some other congeners like 1.2.3.4.6.7.8-hepta CDD; octa CDD changed but not significantly (p > 0.05)
Trang 5Table 5: Alteration of CDFs and TEQ after detoxification (pg/g lipid)
Study subject (n = 34) Index
p (1-2)
The mean blood concentration of 2.3.7.8-tetra CDF and congeners were 2.3.4.7.8-penta CDF; 1.2.3.4.7.8-hexa CDF; octa CDF of the exposed group significantly decreased after the treatment (p < 0.05)
The decreasing trend in TEQ of dioxins concentration was also seen after detoxification
Table 6: Results of humoral immune indexex before and after detoxification
Concentrations of IgA, IgG and IgM increased significantly after applying the Hubbard detoxification method (p < 0.05)
Trang 6Table 7: Results of cell immune indexes before and after detoxification
After applying detoxification, the indexes estimated the cell immune corresponding strain T lympho including CD3, CD4, CD8 both increase significantly with p < 0.05
Mentioning the immune system of people exposed to dioxin, Phan Thi Phi Phi [2] and some authors agreed that the immune system was affected and the number of
T cell decreased fast and clearly, which is suitable for our study After applying detoxification, the immune indexes increased from low level to the normal level Detoxification based on the principles of Hubbard program may improve and harmonize elements of immune system
CONCLUSIONS
Before applying the Hubbard detoxification,
the mean blood dioxins concentration of the
exposed group was 50.47 ± 130.74 pg/g
lipid; after treatment this index decreased
down to 39.29 ± 99.93 The difference
was significant (p < 0.05) TEQ also
decreased significantly Concentrations of
other congeners had no significant changes
After detoxification, only cholesterol
decreased significantly Other biochemical
indexes showed no change Hematological
indexes of study group before and after
treatment showed no difference (p > 0.05)
Concentrations of IgA, IgG and IgM
increased significantly: IgA from 281.37 ±
188.74 to 303.86 ± 185.11; IgG from
1103.8 ± 223.5 to 1259.1 ± 286.01;
IgM from 121.29 ± 71.35 to 172.59 ± 70.76
Cell immune index: CD3 from 1341.4 ±
454.64 cell/µL to 172.59 ± 70.76 cell/µL;
CD4 from 766.59 ± 279.43 to 969.85 ± 341.93 cell/µL; CD8 from 443.53 ± 194.22 cell/µL to 555.06 ± 178.39 cell/µL
We primarily recognised that non-specific detoxification based on the principles of Hubbard program can eliminate dioxins from the body and improve some immune indexes of people exposed to agent orange/dioxin
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