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14 A the partial oxidation of glucose with organic molecules serving as electron acceptors B the complete catabolism of glucose to CO2 and H2O C the partial reduction of glucose to pyruv

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Test Bank for Microbiology An Introduction 11th Edition

by Tortora

Chapter 5

Exam

Name _

MULTIPLE CHOICE Choose the one alternative that best completes the statement or answers the question 1) Which of the following compounds is NOT an enzyme? 1) A) coenzyme A B) cellulase C) ?-galactosidase D)

sucrase E) dehydrogenase Answer: A

Explanation: A) B) C) D) E) 2) Which of the following statements about

photophosphorylation is FALSE? 2) A) Energy from oxidation reactions is used to generate ATP from ADP B) Light liberates an electron from

chlorophyll C) It occurs in photosynthesizing cells D) The oxidation of carrier molecules releases energy E) It requires CO2 Answer: E

Explanation: A) B) C) D) E) 3) Gallionella bacteria can get energy from the reaction Fe2+ Fe3+ This reaction is an example of 3) A) the Calvin-Benson cycle B) reduction C) fermentation D) oxidation E) photophosphorylation Answer: D

Explanation: A) B) C) D) E) 1

4)

A bacterial culture grown in a glucose-peptide medium causes the pH to

increase The bacteria are

most likely 4) A) oxidizing the glucose B) using the peptides C) fermenting the glucose D) not growing Answer: B

Explanation: A) B) C) D) 5) Which of the following statements about substrate-level phosphorylation is FALSE? 5) A) It occurs in glycolysis B)

It involves the direct transfer of a high-energy phosphate group from an

intermediate

metabolic compound to ADP C)

The oxidation of intermediate metabolic compounds releases energy that is used to generate

ATP D) No final electron acceptor is required E) It occurs in the Krebs cycle

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Answer: C

Explanation: A) B) C) D) E) 6) Which organism is NOT correctly matched to

its energy source? 6) A) chemoautotroph – Fe2+

B) chemoheterotroph – glucose C) chemoautotroph-NH3

D) photoheterotroph – light E) photoautotroph –

CO2 Answer: E

Explanation: A) B) C) D) E) 7) Which of the following is the best definition of

oxidative phosphorylation? 7) A)

A proton gradient allows hydrogen ions to flow back into the cells through

transmembrane

protein channels, releasing energy that is used to generate ATP B)

Electrons are passed through a series of carriers to O2

C) Electrons are passed through a series of carriers to an organic compound

D) ATP is directly transferred from a substrate to ADP Answer:

A Explanation: A) B) C) D) 2

8) Which of the following statements regarding the glycolysis pathway is

FALSE? 8) A) Two NADH molecules are generated B) One molecule of ATP

is expended C) Two molecules of water are generated D) Two pyruvate

molecules are generated E) Four ATP molecules are generated via

substrate-level phosphorylation Answer: B

Explanation: A) B) C) D)

E) Figure 5.6

9)

The rates of O2 and glucose consumption by a bacterial culture are shown in

Figure 5.6 Assume a

bacterial culture was grown in a glucose medium without O2 Then O2

was added at the time

B) these bacteria cannot grow anaerobically C) aerobic metabolism is more

efficient than fermentation D) these bacteria get more energy anaerobically

Answer: C

Explanation: A) B) C) D) 3

10) If a cell is starved for ATP, which of the following pathways would most

likely be shut down? 10) A) glycolysis B) Kreb’s cycl pathway D) Krebs cycle and

glycolysis Answer: C

Figure 5.7

11)

The graph at the left in Figure 5.7 shows the reaction rate for an enzyme at

its optimum

temperature Which graph shows enzyme activity at a higher temperature?

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11) A) a B) b C) c D)

d Answer: B

Explanation: A) B) C) D) 12)

Which of the following statements are true?

1-Electron carriers are located at ribosomes

2-ATP is a common intermediate between catabolic and anabolic pathways 3-ATP is used for the long-term storage of energy and so is often found in storage granules

4-Anaerobic organisms are capable of generating ATP via respiration

5-ATP can be generated by the flow of protons across protein channels

12) A) 1, 2, 3 B) 1, 3, 4 C) 2, 3, 5 D) 2, 4, 5 E) All of the statements are

true Answer: D

13) Fatty acids are oxidized in 13) A) the pentose phosphate pathway B) the electron transport chain C) the Entner-Doudoroff pathway D) the Krebs

cycle E) glycolysis Answer: D

Explanation: A) B) C) D) E) 14) Which of the following is the best definition

of fermentation? 14) A) the partial oxidation of glucose with organic

molecules serving as electron acceptors B) the complete catabolism of

glucose to CO2 and H2O

C) the partial reduction of glucose to pyruvic acid D) the production of

energy by oxidative-level phosphorylation E) the production of energy by both substrate and oxidative phosphorylation Answer: A

Explanation: A) B) C) D) E) 15) What is the fate of pyruvic acid in an

organism that uses aerobic respiration? 15) A) It is reduced to lactic acid B) It

is catabolized in glycolysis C) It reacts with oxaloacetate to form citrate D) It

is converted into acetyl CoA E) It is oxidized in the electron transport chain Answer: D

Explanation: A) B) C) D) E) 16) A urease test is used to identify

Myobacterium tuberculosis because 16) A) M bovis can cause tuberculosis B) some bacteria reduce nitrate ion C) urea accumulates during tuberculosis D) M tuberculosis produces urease E) urease is a sign of tuberculosis

Answer: D Explanation: A) B) C) D) E) 5

17) In green and purple bacteria, electrons to reduce CO2 can come from 17) A) H2O B) H2S C) C6H12O6

D) chlorophyll E) CO2

Answer: B

Explanation: A) B) C) D) E) 18) Which of the following uses CO2 for carbon and H2 for energy? 18) A) chemoheterotroph B) chemoautotroph C)

photoheterotroph D) photoautotroph Answer: B

Explanation: A) B) C) D) 19) Which of the following is NOT necessary for

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respiration? 19) A) quinones B) flavoproteins C) oxygen D) cytochromes E)

a source of electrons Answer: C

Explanation: A) B) C) D) E) 20) Which of the following statements regarding the Entner-Doudoroff pathway is TRUE? 20) A) ATP is generated B) NADH is generated C) It involves the pentose phosphate pathway D) It involves

glycolysis E) NADH and ATP are generated Answer: A Explanation: A) B) C) D) E) 6

Figure 5.8

D) ATP synthase E) cytoplasm Answer: B

Explanation: A) B) C) D) E) 22) Cyanobacteria are a type of 22)

A) photoheterotroph B) chemoautotroph C) chemoheterotroph

D) photoautotroph Answer: D

Explanation: A) B) C) D) 23) A strictly fermentative bacterium produces

energy 23) A) by aerobic respiration only B) only in the absence of oxygen C) by glycolysis only D) only in the presence of oxygen E) by fermentation or aerobic respiration Answer: C

Explanation: A) B) C) D) E)

7 Figure 5.4

24) How is ATP generated in the reaction shown in Figure 5.4? 24)

A) photophosphorylation B) oxidative phosphorylation C)

substrate-level phosphorylation D) fermentation E) glycolysis Answer: C

Explanation: A) B) C) D) E) 25)

Which one of the following would you predict is an allosteric inhibitor of

the Krebs cycle enzyme,

?-ketoglutarate dehydrogenase? 25) A) ?-ketoglutaric acid B) ADP C) NADH D) citric acid E) NAD+

Answer: C

Explanation: A) B) C) D) E) 26) In noncyclic photophosphorylation, O2

is released from 26) A) H2O B) chlorophyll C) sunlight D) CO2

E) C6H12O6

Answer: A

27) Which of the following uses glucose for carbon and energy? 27) A)

chemoheterotroph B) photoheterotroph C) photoautotroph D)

chemoautotroph Answer: A

Figure 5.3

28)

How would a noncompetitive inhibitor interfere with a reaction involving the

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enzyme shown in

Figure 5.3? 28) A) It would bind to a B) It would bind to b C) It would bind to

c D) It would bind to d E) The answer cannot be determined based on the information provided Answer: B

Figure 5.1

29) Which compound is being reduced in the reaction shown in Figure 5.1? 29) A) NADH B) NAD+

C) ?-ketoglutaric acid and NAD+

D) isocitric acid and ?-ketoglutaric acid E) NADH and isocitric acid Answer: B Explanation: A) B) C) D) E) 10

Figure 5.8

30) Refer to Figure 5.8 In aerobic respiration, where is water formed? 30) A)

a B) b C) c D) d E) e

Answer: D

Figure 5.2

31) What type of reaction is in Figure 5.2? 31) A) reduction B) transamination C) dehydrogenation D) decarboxylation E) oxidation Answer: B Explanation: A) B) C) D) E) 32) Which of the following statements about beta oxidation is FALSE? 32) A) It is a method of catabolizing fatty acids B) It is a step in glycolysis C) It is used in petroleum degradation D) It involves the formation

of acetyl-CoA E) It involves the formation of 2-carbon units Answer: B

Figure 5.8

33) What is the most acidic place in Figure 5.8? 33) A) a B) b C) c D) d E) e Answer: A

Which of the following is TRUE about this reaction?

NO3

-+ 2H+

Pseudomonas NO2

– + H2O

Nitrate ion Nitrite ion

34) A) This process occurs anaerobically B) This process requires O2 C) This process requires the electron transport system D) This process requires light E) This process requires O2 and the electron transport system Answer: A

35)

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Microorganisms that catabolize sugars into ethanol and hydrogen gas would most likely be

categorized as 35) A) aerobic respirers B) homolactic fermenters C) alcohol fermenters D) anaerobic respirers E) heterolactic fermenters Answer: E Explanation: A) B) C) D) E)

Figure 5.8

C) protons D) energy E) electrons Answer: C

Explanation: A) B) C) D) E) 37) Which of the following statements regarding metabolism is FALSE? 37) A) Heat may be released in both anabolic and catabolic reactions B) ADP is formed in anabolic reactions C) ATP is

formed in catabolic reactions D) Anabolic reactions are degradative

Answer: D Explanation: A) B) C) D) 14

38) The advantage of the pentose phosphate pathway is that it produces all

of the following EXCEPT 38) A) precursors for the synthesis of glucose B) NADPH C) three ATPs D) precursors for the synthesis of amino acids E) precursors for nucleic acids Answer: C

Explanation: A) B) C) D) E) 39) Which of the following has

bacteriochlorophylls and uses alcohols for carbon? 39) A) chemoautotroph B) photoautotroph C) photoheterotroph D) chemoheterotroph Answer: C Explanation: A) B) C) D) 40)

Assume you are growing bacteria on a lipid medium that started at pH 7 The action of bacterial

lipases should cause the pH of the medium to 40) A) stay the same

B) decrease C) increase Answer: B

Explanation: A) B) C) 15

Figure 5.8

41) In Figure 5.8, where is ATP produced? 41) A) a B) b C) c D) d E)

e Answer: E

An enzyme, citrate synthase, in the Krebs cycle is inhibited by ATP This is

an example of all of the

following EXCEPT 42) A) feedback inhibition B) competitive inhibition

C) noncompetitive inhibition D) allosteric inhibition Answer: B

Explanation: A) B) C) D) 16

Figure 5.5

43)

Which of the graphs in Figure 5.5 best illustrates the activity of an enzyme that is saturated with

substrate? 43) A) a B) b C) c D) d E) e

Answer: C

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Assume you are working for a chemical company and are responsible for

growing a yeast culture

that produces ethanol The yeasts are growing well on the maltose medium

but are not producing

alcohol What is the most likely explanation? 44) A) The maltose is toxic B)

O2 is in the medium C) The temperature is too low D) The temperature is too

high E) Not enough protein is provided Answer: B

Explanation: A) B) C) D) E) 45) Which of the following statements about

B) It yields lower amounts of ATP when compared to aerobic respiration C)

It involves the reduction of an organic final electron acceptor D) It generates

ATP E) It requires cytochromes Answer: C

TRUE/FALSE Write ‘T’ if the statement is true and ‘F

46) An apoenzyme that loses its coenzyme subunit will be non-functional 46)

Answer: True False Explanation: 47) Glycolysis is utilized by cells in both

respiration and fermentation 47) Answer: True False Explanation: 48) The

pentose phosphate pathway can be characterized as an anabolic pathway

48) Answer: True False Explanation: 49) Both respiration and photosynthesis

require the use of an electron transport chain 49) Answer: True False

Explanation: 50) Both respiration and photosynthesis use water molecules for

the donation of hydrogen ions 50) Answer: True False Explanation: 51)

Catabolic reactions are generally degradative and hydrolytic 51) Answer:

True False Explanation: 52)

Once an enzyme has converted substrates into products, the active site

reverts back to its original

form 52) Answer: True False Explanation: 53)

The use of enzymes is necessary to increase the activation energy

requirements of a chemical

reaction 53) Answer: True False Explanation: 54) Carbon fixation occurs

during the light-independent phase of photosynthesis 54) Answer: True False

Explanation: 55) In general, ATP is generated in catabolic pathways and

expended in anabolic pathways 55) Answer: True False Explanation: ESSAY

Write your answer in the space provided or on a separate sheet of paper 56)

Rhodopseudomonas is an anaerobic photoautotroph that uses organic

compounds as an electron donor It is also

capable of chemoheterotrophic metabolism Diagram the metabolic

pathways of this bacterium Answer: 18

57)

Streptococcus lacks an electron transport chain How does this bacterium

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reoxidize NADH? Where is the NADH

formed? Answer: 58) Differentiate the following two laboratory tests: starch hydrolysis and starch fermentation Answer: 59)

You look in the refrigerator and find some orange drink you had forgotten

was there The drink now has an

the drink? Answer: 60) Compare and contrast photophosphorylation and

oxidative phosphorylation Answer: 19

Answer Key

Testname: C5

1) A

2) E

3) D

4) B

5) C

6) E

7) A

8) B

9) C

10) C

11) B

12) D

13) D

14) A

15) D

16) D

17) B

18) B

19) C

20) A

21) B

22) D

23) C

24) C

25) C

26) A

27) A

28) B

29) B

30) D

31) B

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32) B

33) A

34) A

35) E

36) C

37) D

38) C

39) C

40) B

41) E

42) B

43) C

44) B

45) C

46) TRUE 47) TRUE 48) FALSE 49) TRUE 50) FALSE

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