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THE CRITICAL INCIDENTS IN THE COMBINED ANESTHESIA DURING MAJOR ABDOMINAL SURGERY IN ELDERRY AND OLD PATIENTS ROLE PREOPERATIVE LEVEL OF WAKEFULNESS

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CONCLUSIONPostoperative spirometry is not affected by PEEP and RM during intraoperative ventilation for open abdominal surgery in nonobese patients at a high risk of PPCs, but rather is

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ORIGINAL ARTICLE

Ventilation with high versus low peep levels during

general anaesthesia for open abdominal surgery does not affect postoperative spirometry

A randomised clinical trial

Tanja A Treschan, Maximilian Schaefer, Johann Kemper, Bea Bastin, Peter Kienbaum,

Benedikt Pannen, Sabrine N Hemmes, Marcelo G de Abreu, Paolo Pelosi and Marcus J Schultz, for the PROVEMNetwork Investigators

BACKGROUNDInvasive mechanical ventilation during

gen-eral anaesthesia for surgery typically causes atelectasis and

impairs postoperative lung function

OBJECTIVE We investigated the effect of intraoperative

ventilation with high positive end-expiratory pressure (PEEP)

and recruitment manoeuvres (RMs) on postoperative

spiro-metry

DESIGNThis was a preplanned, single-centre substudy of

an international multicentre randomised controlled trial, the

PROVHILO trial

SETTING University hospital from November 2011 to

January 2013

PATIENTSNonobese patients scheduled for major

abdomi-nal surgery at a high risk of postoperative pulmonary

com-plications (PPCs)

INTERVENTION Intraoperative low tidal volume ventilation

with PEEP levels of 12 cmH2O and RM (the high PEEP

group) or with PEEP levels of 2 cmH2O or less without RM

(the low PEEP group)

MAIN OUTCOME MEASURES Time-weighted averages

(TWAs) of the forced expiratory volume in 1 s (FEV1)

and the forced vital capacity (FVC) up to postoperative day five

RESULTSThirty-one patients were allocated to the high PEEP group and 32 to the low PEEP group No post-operative spirometry test results were available for 6 patients In both groups, TWA of FEV1 and FVC until postoperative day five were lower than preoperative values Postoperative spirometry test results were not different between the high and low PEEP group; Data are median [interquartile range], TWA FVC 1.8 [1.6 to 2.4] versus 1.7 [1.2 to 2.4] l (P¼ NS) and TWA FEV1 1.2 [1.1

to 2.5] versus 1.2 [0.9 to 1.9] l (P¼ NS) Patients who developed PPCs had lower FEV1 and FVC on postopera-tive day five; 1.1 [0.9 to 1.6] versus 1.6 [1.4 to 1.9] l (P¼ 0.001) and 1.6 [1.2 to 2.6] versus 2.3 [1.7 to 2.6] l (P¼ 0.036), respectively

CONCLUSIONPostoperative spirometry is not affected by PEEP and RM during intraoperative ventilation for open abdominal surgery in nonobese patients at a high risk of PPCs, but rather is associated with the development of PPCs

TRIAL REGISTRATIONClinicalTrials.gov NCT01441791 Published online 16 March 2017

From the Department of Anesthesiology, Du¨sseldorf University Hospital, Medical Faculty of Heinrich-Heine University, Du¨sseldorf, Germany (TAT, MS, JK, BB, PK, BP), The Department of Anesthesiology, The Academic Medical Center, Amsterdam, The Netherlands (SNH), The Department of Anesthesiology and Intensive Care, University Hospital Carl Gustav Carus, Dresden, Germany (MGA), The Department of Surgical Sciences and Integrated Diagnostics, IRCCS San Martino IST, University of Genoa, Genoa, Italy (PP) and the Laboratory of Experimental Intensive Care and Anesthesiology (LEICA), and the Department of Intensive Care, The Academic Medical Center, Amsterdam, The Netherlands (MJS) for the PROVE Network Investigators

Correspondence to Tanja A Treschan, MD, CLiPS – Clinical Trials – Patient–centred Studies, Department of Anesthesiology, Du¨sseldorf University Hospital, Moorenstrasse 5, 40225 Du¨sseldorf, Germany

E-mail: tanja.treschan@med.uni-duesseldorf.de

*PROVE network = the PROtective VEntilation Network (www.provenet.eu).

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Copyright © European Society of Anaesthesiology Unauthorized reproduction of this article is prohibited.

Introduction

Invasive mechanical ventilation during general

anaesthe-sia for surgery typically results in atelectasis as well as

reduced lung volume due to a cephalad shift of the

diaphragm and a decreased muscle tone after induction

of anesthesia.1 In particular, in patients undergoing

abdominal surgery, the risk of atelectasis increases the

closer the incision is to the diaphragm.2,3 Although

intraoperative atelectasis impairs intraoperative

oxygen-ation,4more importantly, atelectasis often continues into

the postoperative period, changing the mechanics of

regional lung aeration and impairing the postoperative

recovery of pulmonary function.5Accordingly, atelectasis

could predispose to the development of postoperative

pulmonary complications (PPCs), including hypoxemia

and pneumonia,2with an increased risk of postoperative

morbidity and mortality.6

Postoperative regeneration of pulmonary function could

depend, at least in part, on the intraoperative ventilation

strategy Indeed, a significantly greater reduction in

peri-operative and postperi-operative lung volumes is seen with

general anaesthesia as compared with spinal anesthesia,7

and with controlled rather than with assisted modes of

ventilation.8 Furthermore, so-called ‘protective

intrao-perative ventilation’ that uses a combination of low tidal

volumes and positive end-expiratory pressure (PEEP)

and recruitment manoeuvres (RMs) could prevent the

development of PPCs.9 – 12 However, the protective

role of PEEP in preventing PPCs was challenged

recently.13,14

Despite protective intraoperative ventilation, PPCs occur

in up to 39% of patients.9,10,13 Risk scores, using

pre-operative characteristics, for the development of PPCs

and early recognition of patients who develop PPCs could

contribute to an improved patient outcome.15,16As with

preoperative spirometry to predict PPCs,17,18

postopera-tive spirometry could be a useful tool to monitor

post-operative recovery of lung function.7,9Therefore, in this

substudy of the international multicentre, randomised

controlled ‘PROtective Ventilation using HIgh versus

LOw PEEP’ (PROVHILO) trial,13,19 in which

intrao-perative ventilation with a high level of PEEP

(12 cmH2O) and RMs was compared with a low level

of PEEP (2 cmH2O) without RMs during general

anaesthesia for planned open abdominal surgery in

non-obese patients at risk of PPCs, we tested the hypothesis

that postoperative spirometry results would be modified

by the intraoperative level of PEEP In addition, we

compared postoperative spirometry test results in

patients who did and who did not develop PPCs

Materials and methods

Ethical approval and informed consent

This was a preplanned substudy of the recently

pub-lished PROVHILO trial.13,19This single-centre substudy

was performed at the Du¨sseldorf University Hospital,

Du¨sseldorf, Germany Patients at our institution were included from November 2011 until January 2013 The original trial was approved by the Institutional Review Boards of the Academic Medical Center (AMC), Amster-dam, The Netherlands, and on 5 July 2011 by the Medizinischen Fakulta¨t der Heinrich–Heine Universita¨t Du¨sseldorf, Du¨sseldorf, Germany (Study number 3664, chairperson Prof Kro¨ncke), and registered at Clinical-Trials.gov NCT01441791 The latter additionally approved this substudy as an amendment Participants had to give written informed consent prior to participa-tion for any procedure related to the original trial and this substudy

Design of the original trial

In the PROVHILO trial, nonobese patients with an intermediate or high risk of PPCs according to the Assess Respiratory Risk in Surgical Patients in Catalonia (ARIS-CAT) score15,16 and who were scheduled for open abdominal surgery under general anaesthesia were ran-domly assigned to intraoperative ventilation with high levels of PEEP and RMs (12 cmH2O; the ‘high PEEP group’) or ventilation with lower levels of PEEP without RMs (<2 cmH2O; the ‘low PEEP group’) In the high PEEP group, patients received RMs at the following times: after intubation at the start of ventilation; before tracheal extubation; after each accidental disconnection from the ventilator RMs were performed as follows: peak inspiratory pressure limit is set at 45 cmH2O; tidal volume

is set at 8 ml kg1predicted body weight (PBW), respir-atory rate at 6 to 8 breaths min1 (or lowest respiratory rate that the anaesthesia ventilator allows), and PEEP is set at 12 cmH2O; inspiratory to expiratory (I:E) ratio is set

at 1 : 2; tidal volumes are increased in steps of 4 ml kg1 PBW until a plateau pressure of 30 to 35 cmH2O is attained; three breaths are administered with a plateau pressure of 30 to 35 cmH2O; peak inspiratory pressure limit, respiratory rate, I:E ratio, and tidal volume are reset

to the settings preceding each recruitment manoeuver

FiO2remained unchanged during RMs

Patients were excluded for the following reasons: a planned laparoscopic abdominal procedure, pregnancy,

a BMI more than 40 kg m2, severe cardiac or pulmonary

or other comorbidities, and if they were participating in other interventional studies This substudy had no additional exclusion criteria

In both arms of the trial, patients were ventilated with a tidal volume of 8 ml kg1PBW, FiO2was 0.4 or higher to maintain SpO2 at least 92%, the respiratory rate was adjusted to maintain end-tidal CO2 between 4.67 and 6.0 kPa; and the I:E ratio was 1 : 2 Patients, and post-operative investigators who assessed whether or not a patient developed one or more PPCs, were blind to the intraoperative ventilation strategy As recommended by the protocol and according to institutional routine, in this

Eur J Anaesthesiol 2017; 34:534–543

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substudy, extubation was undertaken without suctioning

of the trachea Patients received additional oxygen as

deemed necessary by the attending anaesthesiologist and

were typically positioned supine with heads elevated to a

maximum of 308 when extubated

The primary endpoint of the PROVHILO trial was a

composite of PPCs within the first five postoperative

days These PPCs consisted of an unexpected need for

supplementary oxygen, severe hypoxemia,

bronchos-pasm, suspected pulmonary infection, a pulmonary

infil-trate, aspiration pneumonitis, development of ARDS,

atelectasis, pleural effusion, pulmonary oedema caused

by cardiac failure or pneumothorax The definition of

every complication is presented in the online

Supple-ment Table 1, http://links.lww.com/EJA/A115

Design of the present substudy

Preoperative and postoperative spirometry on

postopera-tive days 1, 3 and 5 was performed after detailed

instruc-tions to participating patients Patients were requested to

rate their pain, while at rest in the supine position with

308 upper body elevation, on a numeric rating scale of 0 to

10 (from 0, no pain, to 10, maximum pain) Spirometric

testing was only performed if pain scores at rest were 3 or

less; otherwise, analgesia was optimised before

spiro-metric measurements According to institutional

proto-col, a continuous infusion of ropivacaine 0.2% through a

thoracic epidural catheter was used for analgesia

Additional bolus doses and rate adjustments were made

by the pain service according to the patients’ needs For

patients without epidural catheters, piritramid was

admi-nistered intravenously as bolus doses or by

patient-con-trolled analgesia pumps Spirometry was performed in

accordance with the American Thoracic Society’s

stan-dards20using a single pneumotachograph (SpiroPro,

Jae-ger, Wu¨rzburg, Germany) with the patient in the supine

position with 308 upper body elevation Each

measure-ment was performed three times at each timepoint and

the best value was selected for inclusion in the analysis

The postoperative investigators who performed the

spirometry were blind as to the intraoperative ventilation

strategy

The primary endpoints of this substudy were the

post-operative time-weighted averages (TWAs) of both the

forced expiratory volume in 1 s (FEV1) and the forced

vital capacity (FVC), up to postoperative day 5 TWAs

were calculated for each patient as the area under the

curve for FVC and FEV1 measurements divided by the

follow-up duration in hours

Power calculation

We intended to include all the participants within our

centre from the original trial into this substudy

Con-sequently, the number of patients who could be included

was restricted to the recruitment period of the original

trial An a priori sample-size estimate indicated that a

minimum of 57 patients per group would provide an 80% chance of detecting a 20% difference in the TWAs of FVC and FEV1 from a presumed postoperative TWA

of FVC of 1.6 0.5 l with a corresponding TWA of FEV1

of 1.2 0.4 l, with an alpha error level of 2.5% for com-bined outcomes On the same basis, a minimum of 25 patients per group would provide an 80% chance of detecting a 30% difference

Analysis plan

We first compared results of postoperative spirometric measurements between patients ventilated with high PEEP with RM to those ventilated with low PEEP without RM Then, postoperative spirometry results were compared between patients who developed PPCs and those who did not

In two posthoc analyses, we evaluated whether intrao-perative pulmonary compliance or the site of the surgical incision in combination with high PEEP and RM or low PEEP without RM influenced outcomes in our substudy Therefore, we first compared spirometric results in patients with an intraoperative pulmonary compliance more than 50 ml cm1 H2O (high compliance) to those with a compliance 50 ml cm1H2O or less (low compli-ance) The cut-off was based on the median intraopera-tive pulmonary compliance in all substudy patients We then further subdivided the spirometric results of patients with high or low compliance by ventilation strategy, that is low PEEP without RM versus high PEEP with RM Secondly, we compared postoperative spiro-metry results of patients who had the incision closer to the diaphragm (i.e upper abdominal surgery) with those with the incision at a distance from the diaphragm (i.e lower abdominal surgery) and also further subdivided these spirometric results by ventilation strategy, that is low PEEP without RM or high PEEP with RM

Statistical analysis

Data are presented as absolute values, means with stan-dard deviation or medians with interquartile range, as appropriate Analyses were performed on an intention-to-treat basis We used the Kolmogorow –Smirnow test to test the distribution of data and the two-tailed Fishers’ exact test, Student’s t-test or Mann–Whitney U tests

as appropriate for comparison between groups For analysis within groups, the Wilcoxon rank-sum test was performed

The IBM SPSS Statistics Versions 21 and 22 (IBM Deutschland GmbH, Ehningen, Germany) were used

To take account of two primary endpoints, FEV1 and FVC, a Bonferroni-corrected P value less than 0.025 was considered to be statistically significant for the two primary outcomes TWA of FEV1 and FVC For second-ary outcomes, which were exploratory, P value less than 0.05 was considered to be statistically significant

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Copyright © European Society of Anaesthesiology Unauthorized reproduction of this article is prohibited.

Results

Substudy patients and occurrence of postoperative

pulmonary complication

All 63 patients enrolled in the PROVHILO trial in

Du¨sseldorf participated in this substudy; 31 and 32

patients were randomised to the high and the low PEEP

group, respectively (Fig 1) One patient in the high

PEEP group received ventilation with a PEEP of

5 cmH2O for 3 out of 4 hours by mistake; PEEP of

12 cmH2O was thus only applied for the last hour

Accord-ing to the intention-to-treat analysis, this patient

remained in the high PEEP group Six patients were

excluded from the analysis because postoperative

spiro-metry results could not be obtained, leaving 27 in the

high PEEP group and 30 in the low PEEP group for the

final analysis (Fig 1)

The occurrence of PPC in the substudy was high (24/

57¼ 42%) but comparable to that found in the original

trial (346/880¼ 39%, Chi-squared P ¼ 0.677) A

compari-son between characteristics of patients enrolled in the

original trial and patients in the substudy is provided in

the online supplement (Supplement Table S2, http://

links.lww.com/EJA/A115)

Among patients participating in the substudy, baseline characteristics, including preoperative spirometry results, did not differ between the two randomisation groups ventilated with high or low PEEP (Table 1) The level

of PEEP and peak inspiratory pressure levels were different between the randomisation groups, as was the pulmonary compliance during intraoperative ventilation (Supplement Table S3, http://links.lww.com/EJA/A115)

PPC did not differ between the randomisation groups (Supplement Table S4, http://links.lww.com/EJA/A115)

Postoperatively, the ratio between FEV1/FVC remained within the normal range in the majority of patients [TWA FEV1/FVC¼ 76 (68 to 80)%]

Association between intraoperative ventilation strategy and spirometry results

Spirometry results were unaffected by the intraoperative ventilation strategy: TWA of FVC and FEV1 were not different between the high and low PEEP group [TWA

Fig 1

63 patients underwent randomisation

31 patients were assigned to

high PEEP with RM

1 patient received treatment

other than that allocated

32 patients were assigned to low PEEP without RM

0 patients received treatment other than that allocated

Missing postoperative lung function tests n = 4

27 patients available for

the primary analysis

30 patients available for the primary analysis

Missing postoperative lung function tests n = 2

63 patients underwent preoperative lung function tests

CONSORT diagram of patients Reasons for missing postoperative spirometry were open abdominal wounds (n¼ 2), continued mechanical

ventilation (n ¼ 1) and uncontrolled pain (n ¼ 1) in patients ventilated with high PEEP and continued mechanical ventilation (n ¼ 2) in the low PEEP

group.

Eur J Anaesthesiol 2017; 34:534–543

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Table 1 Baseline characteristics of patients

Male sex – n/N 30 (53%) 13 (48%) 17 (56%) 0.600 Age (years), median [IQR] 57 [44 to 69] 55 [44 to 68] 59 [44 to 69] 0.876 BMI (kg m2), mean (SD) 27  5 26  6 25  5 0.510 Body weight (kg), mean (SD) 77  17 77  16 75  18 0.850 ARISCAT score – median [IQR] 41 [38 to 50] 41 [38 to 51] 41 [34 to 50] 0.462 Intermediate (26 to 44) – % (n/N) 39/57 (68%) 19/27 (70%) 20/30 (67%)

High (>44) – % (n/N) 18/57 (32%) 8/27 (30%) 10/30 (33%)

Smoking status – n/N

Never 26/57 (46%) 13 (48%) 13 (43%) 0.935 Former 11/57 (19%) 5 (19%) 6 (20%)

Current 20/57 (35%) 9 (33%) 11 (37%)

Alcohol status (past 2 weeks) – % (n/N)

None 39/57 (69%) 17 (63%) 22 (73%) 0.492

0 to 2 units of alcohol 15/57 (26%) 9 (33%) 6 (20%)

>2 units of alcohol 3/57 (5%) 1 (4%) 2 (7%)

ASA physical status classification system – % (n/N)

1 10/56 (18%) 5/27 (19%) 5/29 (17%) 0.768

2 26/56 (46%) 12/27 (44%) 14/29 (48%)

3 19/56 (34%) 10/27 (37%) 9/29 (31%)

5

New York Heart Association Classification – % (n/N)

I 46/50 (92%) 21/23 (91%) 25/27 (93%) 0.632

II 4/50 (8%) 2/23 (9%) 2/27 (7%)

Functional status – % (n/N)

Nondependent 54/57 (95%) 25/27 (93%) 29/30 (97%) 0.599 Partially dependent 3/57 (5%9 2/27 (7%) 1/30 (3%)

Totally dependent 0/57 0/28 0/30

History of active cancer – n/N 31/52 (60%) 12/23 (52%) 19/29 (56%) 0.400 History of chronic renal failure – % (n/N) 2/57 (4%) 1/27 (4%) 1/30 (3%) 1.0

COPD – % (n/N) 1/57 (2%) 0/27 1/30 (3%) 1.0

With inhalation therapy 1/56 (2%) 0/27 1/29 (3%) 1.0

With systemic steroids 1/56 (2%) 1/27 (4%) 0/29 0.482 Diabetes mellitus – % (n/N) 6/57 (11%) 4/27 (15%) 2/30 (7%) 0.408 With oral medication 1/4 (25%) 1/3 (33%) 0/1

With insulin therapy 3/4 (75%) 2/3 (67%) 1/1 (100%)

Use of systemic steroids – % (n/N) 5/56 (9%) 3/27 (11%) 2/29 (7%) 0.664 Use of statins – % (n/N) 3/57 (5%) 2/27 (7%) 1/30 (3%) 0.599 Preoperative transfusion – % (n/N) 0/57 0/27 0/30 1.0

Preoperative tests

Haemoglobin (g l1) 129 (22) 128 (23) 131 (21) 0.620 Creatinine (mmol/l) median [IQR] 61 [46 to 61] 61 [46 to 61] 51 [46 to 61] 0.952 Urea, mmol/l, median [IQR] 4.3 [2.6 to 5.6] 4.3 [3.6 to 5.7] 4.5 [3.8 to 5.2] 0.527 White blood cells ( 10 9 l 1 ) median [IQR] 7.2 [5.6 to 9.6] 7.6 [6.2 to 10.2] 7.0 [5.5 to 8.5] 0.275 Preoperative SpO 2 – %, median [IQR] 97 [96 to 98.5] 97 [96 to 99] 96 [96 to 98] 0.145 Abnormalities on chest radiograph – % (n/N) 1/36 (3%) 1/17 (5%) 0/19 0.563 Peri-operative variables

Duration of surgery a (min), mean (SD) 309 (161) 326 (132) 295 (184) 0.473 Surgical procedure – % (n/N)

Antibiotic prophylaxis – % (n/N) 57/57 (100%) 27/27 (100%) 30/30 (100%) 1.0

Type of anaesthesia – % (n/N)

Total intravenous 3/57 1/27 2/30 1.0

Mixed (volatile and intravenous) 54/57 26/27 28/30

Epidural – % (n/N) 42/57 19/27 23/30

thoracic 42/42 (100%) 19/19 (100%) 23/23 (100%) 0.764

Eur J Anaesthesiol 2017; 34:534–543

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Copyright © European Society of Anaesthesiology Unauthorized reproduction of this article is prohibited.

FVC¼ 1.8 (1.6 to 2.4) versus 1.7 (1.2 – 2.4) l (P ¼ 0.792)

and TWA FEV1¼ 1.2 (1.1 to 2.5) versus 1.2 (0.9 to 1.9)] l

(P¼ 0.497) There were also no differences in FVC or

FEV1 between randomisation groups on individual

post-operative days (Fig 2)

Association between the occurrence of postoperative

pulmonary complication and spirometry results

In patients who developed PPCs, FEV1 and FVC values

on postoperative day 5 were about 30% lower than

patients who did not develop PPCs (Fig 3) Compared

with patients who did not develop PPCs, patients who

developed PPCs had longer surgery (supplement Table

5, http://links.lww.com/EJA/A115), received higher tidal

volumes, higher minute ventilation volumes and more

intravenous fluids during surgery (supplement Table 6,

http://links.lww.com/EJA/A115)

Posthoc analyses

Association between intraoperative pulmonary compliance

and spirometry results

On the first postoperative day, spirometry results were

about 40% higher in patients with high pulmonary

com-pliance, but unaffected by PEEP and RM (Fig 4a, b)

Patients with high intraoperative pulmonary compliance

were not different from those with a low compliance

(supplement Table 7, http://links.lww.com/EJA/A115), but more frequently received intraoperative ventilation with high PEEP (supplement Table 8, http://links.lww

com/EJA/A115), but incidence of PPCs was not different (supplement Table 9, http://links.lww.com/EJA/A115)

Association between location of incision and spirometry results

Patients who had upper abdominal surgery were current smokers more frequently and had a longer duration of surgery than patients who had lower abdominal surgery (supplement Table 10, http://links.lww.com/EJA/A115) and received more fluids and transfusions (supplement Table 11, http://links.lww.com/EJA/A115) The inci-dence of PPCs, however, was not different (supplement Table 12, http://links.lww.com/EJA/A115) Postoperative spirometry showed no differences between the high and low PEEP groups, neither in patients who had upper abdominal surgery nor in patients who had lower abdomi-nal surgery (Fig 4c, d)

Discussion

The results of this substudy of a larger randomised controlled trial comparing high with low PEEP during intraoperative ventilation in nonobese patients at risk of PPCs and scheduled for open abdominal surgery can be

Table 1 (continued )

Preoperative spirometry- median [IQR]

FVC (l) 3.7 (2.9 to 4.5) 3.6 (2.7 to 4.6) 3.7 (3.1 to 4.5) 0.587

FEV1 (l) 2.7 (2.1 to 3.3) 2.6 (2.0 to 3.0) 2.8 (2.3 to 3.6) 0.274

FEV1/FVC (%) 73 (69 to 80) 73 (68 to 80) 77 (72 to 79) 0.243

Data are presented as means (SD) Median [IQR] or n/N and proportion %; Calculated as weight (kg)/ height (m) 2 ¼ kg/m 2 ASA, American Society of Anesthesiology;

COPD, chronic obstructive pulmonary disease; FVC, forced vital capacity, FEV1, forced expiratory volume in 1 s; Inhalation therapy for COPD, inhaled bronchodilators

and/or steroids’ SpO2, oxyhaemoglobin saturation measured by pulse oximeter; kg, kilogram; m, meters; n, number of patients; N, total patients a Duration of surgery is the

time between skin incision and closure of the incision.

Fig 2

6.0 litres

litres

4.0

2.0

0.0 PRE DAY 1 DAY 3 DAY 5

6.0

4.0

2.0

0.0 PRE DAY 1 DAY 3 DAY 5

Results of spirometry for patients ventilated with high or low PEEP (a) Forced vital capacity (FVC), and (b) Forced expiratory volume in 1 s (FEV1) in

patients ventilated with high (black bars) and low PEEP (grey bars) during intraoperative ventilation On postoperative day 1, spirometric results were

significantly lower than preoperative values (P < 0.001 in both groups) Compared with day 3, spirometric values increased significantly by day 5

(P ¼ 0.001 for FVC and P ¼ 0.005 for FEV in both groups) Differences between PEEP groups are nonsignificant Data are presented as median

(thick line across box), interquartile ranges (ends of boxes), 90% range (whiskers) and outliers (standalone data points).

Eur J Anaesthesiol 2017; 34:534–543

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summarised as follows: In patients ventilated with a tidal

volume of 8 ml kg1 PBW, postoperative spirometry

results are no different between patients receiving

venti-lation with high PEEP and RM and patients receiving

ventilation with low PEEP without RM: postoperative

spirometry results are abnormal up to postoperative day 5:

occurrence of PPCs seems to be associated with a change

in postoperative spirometry results on postoperative

day 5

To our knowledge, this is one of the largest prospective

randomised controlled studies investigating the

associ-ation between postoperative spirometry changes in

patients undergoing major abdominal surgery and at risk

of developing PPCs

This substudy stopped when the PROVHILO trial

com-pleted recruitment, so we did not recruit the total number

of patients required according to the sample size

calcu-lation, and we had less than 80% power to show a 20%

statistically significant difference between the two

groups A comparison of the median TWAs of the two

treatment groups, high PEEP with RM versus low PEEP

without RM, suggests no difference between FEV1 and a

difference of only 6% in FVC The latter would not be

considered to be of clinical relevance We calculated a

potential effect size based on the means and standard

deviation of each treatment group FVC TWA in the high

PEEP group was 1.89 0.99 versus 1.95  0.68 in the low

PEEP group FEV1 TWA in the high PEEP groups was

1.46 0.8 versus 1.29  0.5 I in the low PEEP group

Thus, dCohen effect size for FVC TWA would be 0.07

[95% confidence interval, 95% CI -0.4 to 0.59] and 0.24

[95% CI -0.7 to 0.27] Our initial hypothesis and sample

size calculation was built on a much stronger effect size of

0.5, which we consider to be clinically relevant

However, we detected significant differences in spiro-metric test results between patients who developed PPCs and those who did not Although this might not seem to

be a surprising result, to our knowledge, postoperative spirometry is not used commonly as a tool to detect or predict PPC It is important to note that our study was not designed to show a direct or timely correlation between spirometric results and the development of PPCs Further studies are needed to determine, whether spiro-metric results could predict or indicate the development

of PPCs at an early stage such that this would allow the initiation of preventive or early therapeutic measures Postoperative spirometry per se might be a useful as a tool

to detect PPCs However, technical and practical reasons limit its utility as a postoperative monitor For instance, pain needs to be adequately controlled and patients need

to be fully awake and compliant

In this substudy and preplanned analysis, we had a unique opportunity to determine the effect of two differ-ent levels of PEEP and RM during intraoperative vdiffer-enti- venti-lation on postoperative lung function test results Its prospective design, the completeness of follow-up and the fact that occurrence of PPCs was scored by assessors who were blind to the intraoperative ventilation strategy helped reduce bias In addition, the definition of PPCs was defined a priori and the patients were similar with regard to their clinical characteristics and type of surgery Lastly, all patients were ventilated with tidal volumes of

8 ml kg1PBW; thus, we were able to assess the effect of PEEP and RMs on postoperative lung function

FVC and FEV1 decreased by more than 50% compared with preoperative values in both randomisation groups This restrictive ventilatory pattern has long been recog-nised after upper abdominal surgery and results from

Fig 3

6.0

(a) FVC

P < 0.05

FEV1 (b)

4.0

2.0

0.0 PRE DAY 1 DAY 3 DAY 5

6.0

4.0

2.0

0.0 PRE DAY 1 DAY 3 DAY 5

P < 0.05

Results of spirometry of patients with or without postoperative pulmonary complications (a) Forced vital capacity (FVC), and (b) Forced expiratory volume in 1 s (FEV1) in patients with (grey bars) or without (black bars) postoperative pulmonary complications On postoperative day 5, spirometric results were significantly lower in patients who developed postoperative pulmonary complications Data are presented as median (thick line across box), interquartile ranges (ends of boxes), 90% range (whiskers) and outliers (standalone data points).

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Copyright © European Society of Anaesthesiology Unauthorized reproduction of this article is prohibited.

reduced ventilatory muscle activity, diaphragmatic

dys-function and decreased lung compliance and is also

influenced by pain levels.21 Although we found

intrao-perative dynamic lung compliance to be significantly

higher in the high PEEP group, this did not protect

against a decline in postoperative lung function These

findings are consistent with the overall results of the

PROVHILO trial, in which the occurrence of PPCs

was high, but not different between patients who

received high PEEP or low PEEP during intraoperative

ventilation.13

The results of the present study support the information that came from two preceding trials of intraoperative ventilation.9,22 In an Italian single-centre, randomised controlled trial of patients scheduled for open abdominal surgery lasting more than 2 h, the FVC and FEV1 on postoperative day 1 were also approximately 50% lower than preoperative values.9However, in that trial, recov-ery of lung function was better in patients ventilated with

a lung-protective ventilation strategy (a PEEP of

10 cmH2O, a low tidal volume of 7 ml kg1 PBW and RM) compared with patients ventilated with a

Fig 4

6.0 litres litres

4.0

2.0

0.0 PRE DAY 1 DAY 3 DAY 5

6.0

4.0

2.0

0.0 PRE DAY 1 DAY 3 DAY 5

6.0 litres litres

4.0

2.0

0.0 PRE DAY 1 DAY 3 DAY 5

6.0

4.0

2.0

0.0 PRE DAY 1 DAY 3 DAY 5

Low High Low High Low High Low High

UpperLower UpperLower UpperLower UpperLower UpperLower UpperLower UpperLower UpperLower

Low High Low High Low High Low High

Results of spirometry in patients with high or low pulmonary compliance and upper or lower abdominal surgery, ventilated with high or low PEEP (a)

Forced vital capacity (FVC), and (b) Forced expiratory volume in 1 s (FEV1) for patients with high or low pulmonary compliance ventilated with high or

low PEEP Patients with low (50 ml cm 1 H 2 O) or high (>50 ml cm1H 2 O) dynamic pulmonary compliance (as indicated by the brackets labelled

‘high’ or ‘low’ in the figures) were ventilated with high PEEP (black bars) or low PEEP (grey bars) On postoperative day 1, patients with high

intraoperative pulmonary compliance had higher FVC (P ¼ 0.021) and FEV1 (P ¼ 0.016) than patients with low intraoperative pulmonary compliance.

Time-weighted average of FVC and FEV1 did not differ between patients with high or low intraoperative pulmonary compliance Data are presented

as median (thick line across box), interquartile ranges (ends of boxes), 90% range (whiskers) and outliers (standalone data points) (c) Forced vital

capacity (FVC), and (d) Forced expiratory volume in 1 s (FEV1) for patients with upper or lower abdominal surgery ventilated with high or low PEEP.

Patients undergoing upper or lower abdominal surgery (indicated by brackets labelled ‘upper’ or ‘lower’ in the figures) were ventilated with high

PEEP (black bars) or low PEEP (grey bars) On postoperative day 1, patients with lower abdominal surgery had higher FVC (P ¼ 0.011) and FEV1

(P ¼ 0.018) than patients with upper abdominal surgery Time-weighted average of FVC and FEV1 did not differ between patients with upper or

lower abdominal surgery Data are presented as median (thick line across box), interquartile ranges (ends of boxes), 90% range (whiskers) and

outliers (standalone data points).

Eur J Anaesthesiol 2017; 34:534–543

Trang 9

conventional ventilation strategy (no PEEP, a tidal

volume of 9 ml kg1 PBW, without RM).9In a German

single-centre, randomised controlled trial of patients

undergoing upper abdominal surgery, in which all

patients received a similar level of PEEP and the same

RM, postoperative changes in spirometry results were not

different in patients ventilated with 6 ml kg1PBW

ver-sus 12 ml kg1PBW.22On the basis of the results of these

two preceding trials and the results from the present

study, we suggest that postoperative spirometry changes,

specifically in the time course of lung function recovery,

might be affected by a combination of the two parameters

‘size of intraoperative tidal volume’ along with ‘PEEP’

and ‘RM’, but not solely by changes either in ‘tidal

volume’ or ‘PEEP’ alone

Since publication of the Italian trial mentioned above,

two other randomised trials of intraoperative ventilation

have been published.10,11 In both trials, patients were

randomly assigned to lung-protective ventilation with

low tidal volumes and high PEEP or conventional

venti-lation with high tidal volume and no PEEP Both trials

found fewer pulmonary complications with

lung-protec-tive ventilation The results from the original trial of this

substudy, the PROVHILO trial,13 suggest that high

levels of PEEP with RM do not protect against

devel-opment of PPCs in patients ventilated with low tidal

volumes

Accordingly, a differentiated algorithm for protective

intraoperative mechanical ventilation has recently been

proposed.23In nonobese patients without acute

respirat-ory distress syndrome undergoing open abdominal

surgery, mechanical ventilation should be performed

with tidal volumes of 6 to 8 ml kg1PBW combined with

a low PEEP of 2 cmH2O or less If hypoxemia develops

and hypotension, hypoventilation or other causes have

been excluded, inspiratory oxygen fraction should be

increased first, followed by increase of PEEP and

recruit-ment manoeuvers.23

Of note, high PEEP with RM failed to affect

postopera-tive spirometry results in two subgroups of patients in

which more benefit of high PEEP could be expected, that

is in patients with a lower pulmonary compliance during

intraoperative ventilation and patients who underwent

upper abdominal surgery

Our study was restricted to patients at risk of PPCs who

were scheduled to undergo open abdominal surgery The

majority of patients in our substudy received thoracic

epidural anaesthesia both intraoperatively and

postopera-tively Therefore, the results could be different in other

patient groups We detected differences in spirometric

test results between patients who developed PPCs and

those who did not However, our study was not designed

to show a direct or timely correlation between spirometric

results and the development of PPCs Further studies are

needed to determine whether spirometric results could

predict or indicate the development of PPCs at an early stage, and whether this would allow preventive or early therapeutic measures to be initiated However, even though postoperative spirometry per se might prove to

be useful as a tool to detect PPCs, technical and practical reasons could limit its utility: postoperative pain needs to be adequately controlled, and patients need to be fully awake and compliant Another limita-tion of our study relates to intra-abdominal pressure Intra-abdominal pressure in the postoperative period could interfere with lung function and hence spiro-metry results We did not measure intra-abdominal pressure, and thus cannot evaluate, whether this influ-enced our results

With the knowledge of our results, the question may arise

as to whether patient management during the emergence phase of anaesthesia could influence lung function to such an extent that the consequences of several hours of intraoperative ventilation become negligible We do not know whether extending the application of PEEP into the postoperative period, or prohibiting use of 100% oxygen during extubation would have changed our results Other trials suggest that if there is an effect due to how the emergence phase of anaesthesia is man-aged, this would only have minor consequences.24,25 Interestingly, as part of a protective ventilation strategy, the beneficial effect of RMs might also be questioned.26 The focus of our study was to compare the effects of several hours of ventilation using high levels of PEEP along with RMs with similar periods of ventilation with-out PEEP and RMs

In conclusion, postoperative spirometry test results are not affected by the PEEP level during intraoperative ventilation during anaesthesia for open abdominal surgery in patients at high risk of PPC Spirometry test results on postoperative day five are associated with the development of PPCs during this time period

Acknowledgements relating to this article

Assistance with the study: we thank Renate Babian for her assist-ance with the study.

Financial support and sponsorship: this work was supported by the Department of Anesthesiology, Du¨sseldorf University Hospital, Du¨sseldorf, Germany.

Conflicts of interest: none.

Presentation: none.

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