Nghiên cứu chọn tạo giống hoa đồng tiền (Gerbera jamesonii) bằng phương pháp đột biến kết hợp với nuôi cấy mô tế bào (Luận văn thạc sĩ)

Nghiên cứu chọn tạo giống hoa đồng tiền (Gerbera jamesonii) bằng phương pháp đột biến kết hợp với nuôi cấy mô tế bào (Luận văn thạc sĩ)Nghiên cứu chọn tạo giống hoa đồng tiền (Gerbera jamesonii) bằng phương pháp đột biến kết hợp với nuôi cấy mô tế bào (Luận văn thạc sĩ)Nghiên cứu chọn tạo giống hoa đồng tiền (Gerbera jamesonii) bằng phương pháp đột biến kết hợp với nuôi cấy mô tế bào (Luận văn thạc sĩ)Nghiên cứu chọn tạo giống hoa đồng tiền (Gerbera jamesonii) bằng phương pháp đột biến kết hợp với nuôi cấy mô tế bào (Luận văn thạc sĩ)Nghiên cứu chọn tạo giống hoa đồng tiền (Gerbera jamesonii) bằng phương pháp đột biến kết hợp với nuôi cấy mô tế bào (Luận văn thạc sĩ)Nghiên cứu chọn tạo giống hoa đồng tiền (Gerbera jamesonii) bằng phương pháp đột biến kết hợp với nuôi cấy mô tế bào (Luận văn thạc sĩ)Nghiên cứu chọn tạo giống hoa đồng tiền (Gerbera jamesonii) bằng phương pháp đột biến kết hợp với nuôi cấy mô tế bào (Luận văn thạc sĩ)

VI VI T NAM

PH M TH MAI

: Sinh h c th c nghi m : 60.42.01.14

NG D N KHOA H C

C TH O

i, 12/2015

DANH M VI T T T vi

DANH M C B NG vii

DANH M C B NG vii

DANH M viii

M U 1

p thi t c 1

1.2 M 2

c u c 3

3

U 4

1.1 Ngu n g m th c v t h c c ng ti n 4

ng ti n 6

1.3 n xu ng ti th gi Vi t Nam 8

1.4 T ng quan v u x t bi n t o v t li u kh u trong ch n gi ng 11

T LI U 20

2 u 20

2.2 V t li u 20

a u 21

2.4 N u 21

u 21

2.6 ch theo 25

2.7 li u 25

T QU U TH O LU N 26

t bi n b ng chi u x o gi ng

ti n in vitro 26

u ng c u chi u x n kh ng ti n in vitro qua 5 th h (M1V5) 29

ng c a li u chi u x n c 34

3.4 ng c a li u chi u x n c ng ti n ng ru ng 36

3.5 c c ng ti t bi ng ru ng 40

T LU N NGH 52

4.1 K t lu n 52

4.2 Ki n ngh 52

U THAM KH O 54

PH L C 61

: 6 - Benzyl Amino Purin

c: C ng s

.C ng ti n DT2 sau chi u x 28

. i c a gi ng ti n DT2 sau 2 tu n c y chuy n 31

Kh c a gi ng ti n DT3 sau 2 tu y 33

. nh (gi ng ti n DT3) 33

.C ng ti 35

Bi n d ng ti n DT1 50

Bi n d ng ti n DT2 50

Bi n d ng ti n DT3 51

Bi n d ng ti n DT4 51

Hoa (Gerbera jamesonii)

gi i h n ngu n gen trong t l t bi n t t th p, ch kho ng

- Hoa -

40

.-

-

- 350 kg/n

ng hoa l n nh t (88.600 ha) ti c (59.527 ha), Indonesia (34.000 ha), Nh t B n (21.218 ha), Hoa K (16.400 ha), Brazil (10.285 ha),

800-2000

2500

2500

1500-4000

3000-7000

7000

5000 2006

1.3

Hoa

ng MS b sung 0,02mg BA/l + 1,75 mg IAA/l

Theo Datta (2001) ch i c a 9 gi ng hoa h c x Gamma (3-4 krad),

ng Rosa indoca var Odorata) Gi ng

M u g t bi n b u x tia Gamma

c th c hi n b Girija M c i h c Annamalai, Tamil Nadu,

c th c hi n chi u x tia Gamma ng 20,

kh u cho ch n t o gi ng c ng vi t bi n th c nghi m t vi c chi u xtia Gamma (Co60 i in vitro cho r ng tia Gamma (Co60 nh

10010095,5

10010096,8

10010094,3

10010090,8

Xanh m

Xanh m

Xanh m

Xanh m5

102030

97,394,586,6

98,595,189,2

93,185,880,5

96,391,382,3

Xanh m

Xanh m

Xanh m

Xanh m10

102030

92,785,279,5

93,587,780,6

91,280,873,2

92,381,174,6

102030

79,558,359,2

80,369,150,0

89,362,357,8

80,664,544,2

Tr ng

nh t

Tr ng xanh

Tr ng xanh

Tr ng

nh t30

102030

65,250,032,7

73,357,540,0

69,254,136,7

71,653,039,1

Xanh Tr ng

xanh

Tr ng xanh

Tr ng xanh40

102030

41,220,011,7

42,521,213,3

36,718,811,7

35,916,010,3 xanh xanh

Xanh

nh t

Tr ng xanh 50

102030

30,010,55,3

37,814,27,2

32,010,64,5

30,89,36,5 nh t nh t60

102030

19,200

21,78,30

10,800

12,80070

102030

9,000

10,200

8,100

10,600

-(0 Gy), 10, 20,30 Gy

in vitro 3.2.1

30 2,46 2,47 2,08 2,27

Ch i nh ,

ng c

Ch i nh , Ch i nh

y u, m ng c

Ch i nh , y

Chxanh nh t,

y u

K t qu b ng 3.4 cho th y h s i sau 5 l n c y chuy n u

r (cm)

S r (r )

LSD 0,05 0,06 0,18

Qua k t qu b ng 3.5 cho th y t l ra r , chi r c 4 gi ng

ng ti n u gi m khi li u chi 3.3, 3.4) li i ch ng (0 Gy) t

l ra r t 100% c 4 gi ng Li u chi u 5, 10 Gy, t l ra r c 4 gi t

- 98,1%); li u chi u 20 Gy t l m xu - 91,3%;

t l ra r th p nh t li u 40 Gy (ch 62,0 - 75,3%) c 4 gi u

ch ng (0 Gy) chi t 0,65 - 0,78 cm; s r t 6,7 - 7,2 r m d n khi li u

Chi u (cm)

Chi u (cm)

T l s ng sau tr ng i ch t cao t 98,8 - 99,6% T l s ng sau

tr ng gi m khi li u chi c 4 gi ng gi m t 5,8 - 37,5% u chi u

-

3.5.2

1.Qua k t qu b ng 3.11 cho th y:

12

DT1, DT2,

0,4 cm; 55,3 cm; 62,8 cm; 54,6 cm

-

-

-3.5.3.

60

2

T n s (%)

T n s (%)

Gi ng Lo i bi n d S

Li u chi u

Gi ng Lo i bi n d S

Li u chi u

-D4.116

-.DT2:

a- Gi i ch ng; b- (10 Gy); c- Bi n d c hoa (20 Gy)

c u t o gi ng hoa ng ti n (Gerbera jamesonii) qua k thu t bi n in vitro b ng

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23 Aleksieva A., Ivanova K and Dimitrov St (2003) A new mutant cultivar Mira

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24 Aney AK (2014) Effect of Gamma Irradiation on Floral Morphology and Pollen

Viability in Two Varieties of Pea (Pisum sativum L.), Int J of Life Sciences, 2(3):

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25 Chen L., YangH., LinB., WangY., LiW., Wang D and ZhangF (2010)Effect of

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26 Codd L.E (1979)The story of the Barbestondaisy, Gerbera jamesonii, pp.114.

27 Datta S K., ChakrabartyD., Mandal A K A (2001) Gamma ray induced

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28 Datta S K., MisraP., Mandal A K A (2005) In vitro mutagenesis-a quick

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32 Eun-Jeong Kang, Yu-Mi Lee, Sang Yeop Sung, Bo-Keun Ha, Sang Hoon Kim,

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Irradiation effect on in vitro organogenesis callus growth and plantlet development of Gerbera jamesonii Horticultura Brasileira 30: 252-257

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