DSpace at VNU: Triple-channel portable capillary electrophoresis instrument with individual background electrolytes for the concurrent separations of anionic and cationic species

DSpace at VNU: Triple-channel portable capillary electrophoresis instrument with individual background electrolytes for...

Triple-channel portable capillary electrophoresis instrument with individual

background electrolytes for the concurrent separations of anionic and cationic species

Thanh Duc Mai, Minh Duc Le, Jorge Sáiz, Hong Anh Duong, Israel Joel Koenka,

Hung Viet Pham, Peter C Hauser

PII: S0003-2670(16)30117-9

DOI: 10.1016/j.aca.2016.01.029

Reference: ACA 234369

To appear in: Analytica Chimica Acta

Received Date: 30 November 2015

Revised Date: 12 January 2016

Accepted Date: 16 January 2016

Please cite this article as: T.D Mai, M.D Le, J Sáiz, H.A Duong, I.J Koenka, H.V Pham, P.C Hauser, Triple-channel portable capillary electrophoresis instrument with individual background electrolytes for

the concurrent separations of anionic and cationic species, Analytica Chimica Acta (2016), doi: 10.1016/

j.aca.2016.01.029.

This is a PDF file of an unedited manuscript that has been accepted for publication As a service to our customers we are providing this early version of the manuscript The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

1 Flushing of the injection

interface 10 s 1 Open 2 Off Off

2 Flushing of the capillary 180 s 1 Open 1 Off Off

3 Flushing of the HV

interface 5 s 2 Closed 2 Off Off

4 Aspiration of sample into

the injection interface 5 s 1 Closed 2 Off On

5 Hydrodynamic injection variable 1 Closed 1 Off Off

6 Flushing of the injection

interface 5s 1 Open 2 Off Off

7 Electrophoretic separation variable 1 Closed 2 On Off

8 Flushing of the injection

interface 10 s 1 Open 2 Off Off

9 Flushing of the capillary 180 s 1 Open 1 Off Off

r 2

LOD b (µM)

Precision of Migration Time (% RSD) c (n = 4)

Precision of Peak Area (% RSD) c (n = 4) Channel 1: Inorganic cations

Compressed

air

Regulating valve

Injection manifold

Sample GND

W Capillary

HV

Pt

HV interface

Safety cage

C 4 D

W

Vent V1 1 2

BGE

BGE

Pt

Pressure-limiting tubing

BGE

600 500

400 300

12 3 4 5 6 7 8 9 10

450 400

350 300

250 200

300 250

300 250

350 300

250 200

350 300

250 200

K+

Ca2+

Na+Mg2+

450 400

350 300

250 200

300 250

300 250

600 500

400 300

1

2

5 6 7

8 9 3

*

800 700

600 500

400 300

2 3

7 8 94

800 700

600 500

400 300

3 6 9

400 350

300 250

300 250

200 150

Ace

*

800 700

600 500

400 300

5 2 1

6

*

800 700

600 500

400 300

4 6

1

Figure 5

450 400

350 300

250 200

300 250

200 150

Cyc Sac

400 350

300 250

200 150

Ace Sac

450 400

350 300

250 200

NH4+K+

Ca2+Na+Mg2+

800 700

600 500

400 300

4 6 8 9

*

450 400

350 300

250 200

• Separate background electrolytes allow individual optimization

• The instrument is compact and field portable