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Publisher: Taylor & Francis
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Journal of Essential Oil Research
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Volatile Constituents of the Leaf Oil of Alchornea tiliifolia (Benth.) Muell (Family Euphorbiaceae) from Vietnam
Nguyên Anh Dũng a
, Trân Đình Thăng b
, Hoàng Văn Lų'u b
& Nguyên Xuân Dũng c a
Faculty of Biology , Vinh University , 182, Le Duan, Vinh, Vietnam
b
Faculty of Chemistry , Vinh University , 182, Le Duan, Vinh, Vietnam
c
Faculty of Chemistry , College of Natural Science, Hanoi National University , 19-Le Thanh Tong, Hanoi, Vietnam
Published online: 09 Dec 2011
To cite this article: Nguyên Anh Dũng , Trân Đình Thăng , Hoàng Văn Lų'u & Nguyên Xuân Dũng (2009) Volatile
Constituents of the Leaf Oil of Alchornea tiliifolia (Benth.) Muell (Family Euphorbiaceae) from Vietnam, Journal of Essential Oil Research, 21:1, 1-2, DOI: 10.1080/10412905.2009.9700091
To link to this article: http://dx.doi.org/10.1080/10412905.2009.9700091
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Trang 2Received: February 2005 Revised: December 2006 Accepted: December 2006
Volatile Constituents of the Leaf Oil of Alchornea
tiliifolia (Benth.) Muell (Family Euphorbiaceae) from
Vietnam
Nguyˆ˜en Anh D ˜ung,
Faculty of Biology, Vinh University, 182- Le Duan, Vinh, Vietnam
Tr ˆan Ðình Th ˘´ang and Hoàng V ˘an L ·u’u,
Faculty of Chemistry, Vinh University, 182- Le Duan, Vinh, Vietnam
Nguyˆ˜en Xuân D ˜ung,*
Faculty of Chemistry, College of Natural Science, Hanoi National University, 19-Le Thanh Tong, Hanoi, Vietnam
Abstract
The leaf oil of Alchornea tiliifolia (Benth.) Muell produced from plants growing in Pu Mat National Park,
Nghean province, Vietnam was prepared by steam distillation The oil was analyzed by GC and GC/MS A total of 45 compounds were identified, of which b-pinene (31.4%), linalool (17.0%) and b-caryophyllene (10.7%) were found
to be major components
Key Word Index
Alchornea tiliifolia, Euphorbiaceae, essential oil composition, b-pinene, linalool, b-caryophyllene.
1041-2905/09/0001-01$14.00/0 —© 2009 Allured Business Media
J Essent Oil Res., 21 (January/February 2009)
*Address for correspondence
Introduction
The Euphorbiaceae family consists of about 317 genera and
5000 species which are widespread, primarily in the tropics and
subtropics, poorly represented in temperate regions (1) About
70 genera and 460 species are distributed in Vietnam (2)
Many species of Euphorbiaceae are of economic
impor-tance, such as sources of medicine, provisions, paint, rubber,
insecticides, etc (1,3)
Alchornea tiliifolia (Benth.) Muell (Local name: Dom
dom la day) (syn Stipellaria tiliifolia Benth.) is one of the 60
species belong to the genus Alchornea, distributed in Son La,
Cao Bang, Quang Ninh, Bac Can, Tuyen Quang, Phu Tho, Ha
Tay provinces, Vietnam (2); India, China, Laos, Cambodia,
Malaysia and Thailand (3)
In the course of the systematic study of Alchornea species
in Indochina, mono-, sesquiterpenes and other components
of the leaf oil of A tiliifolia from Nghean province, Vietnam
have been investigated
Experimental
Source: Alchornea tiliifolia (Euphobiaceae) is a shrub tree
that can grow up to 1–3 m high in Vietnam Leaves were col-lected in Pu Mat National Park, Nghean province A voucher specimen (DD4) has been deposited in the Herbarium of the Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology
Fresh leaves were shredded and their oil was obtained by steam distillation for 3 h at normal pressure, according to the Vietnamese Pharmacopoeia (4) The yield of the fresh leaf oil was 0.22%
GC: About 15 mg of oil, which was dried over anhydrous sodium sulfate, was dissolved in 1 mL of CH3OH (for spec-troscopy or for chromatography)
GC analysis was performed on a HP 6890 Plus Gas chro-matograph equipped with a FID and fitted with a 25 m x 0.25
mm x 0.25 mm film thickness HP-5MS column The analytical conditions were: carrier gas H2with flow rate 35 cm/min,
Trang 3D ˜ung et al.
tor temperature (PTV) 250°C, detector temperature 260°C,
temperature programmed 60°C (2 min hold) to 220°C (10
min hold) at 4°C/min The relative percentage amounts of the
separated compounds were computed from FID data without
the use of correction factors
GC/MS: A Hewlett Packard 6890 Plus Chromatograph
was fitted with the same fused silica HP-5MS capillary
col-lumn The conditions of use were the same as described above except that He was the carrier gas with flow rate 30 mL/min, and there was an interface with a mass spectrometer HP 5972 MSD (70 eV) The temperature was programmed as reported above Component identification was carried out by comparing
MS data with those reported in Wiley Library on Chemstation
HP, and in some cases substances identified from oils known composition and also with standard substances (5–10)
Results and Discussion
The leaf oil of A tiliifolia from Vietnam, obtained in 0.22%
yield was composed entirely of mono- and sesquiterpenes Forty-five components were identified in the oil (Table I) The main components were b-pinene (31.4%), linalool (17.0%) and b-caryophyllene (10.7%) These compounds accounted for 59.1% of the oil These main compounds and other oxygenated components such as 1,8-cineole, nonanal, a-fenchol, borneol, terpinen-4-ol, a-terpineol, myrtenol, neryl formate, nerolidol, caryophyllene oxide, bisabolol oxide B, eudesmol and farnesol contribute to the characteristic aroma of the leaf oil
Other less predominant compounds were a-pinene (8.4%), a-bisabolene (4.3%) and farnesol (5.9%)
To the best of our knowledge until now no publication on chemical composition of the essential oil of this species has been published
Acknowledgments
The authors wish to thank Vu Xuan Phuong, Institute of Ecol-ogy and Biological Resources, Vietnamese Academy of Science and Technology for confirmation of the plant identity and Pu Mat National Park for help collecting samples
References
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Table I Percentage composition of the leaf oil
of Alchornea tiliifolia from Vietnam
trace < 0.1%; * correct isomer not identified.