Phenology, habitat and distribution Curcuma pygmaea occurs in dipterocarp forests and fl owers are either plain green or with a red patch and this varies within a single population.. S
Trang 1Curcuma pygmaea sp nov (Zingiberaceae) from Vietnam and
notes on two related species C parvifl ora and C thorelii
Jana Leong- Š korni cˇ kov á , Otakar Š í da and Tr â` n H u˜ ’u Ð a˘ ng
J Leong- Š korni č kov á (jana_skornickova@seznam.cz), Th e Herbarium, Singapore Botanic Gardens, 1 Cluny Road, 259569 Singapore – O Š í da, Department of Botany, National Museum, Cirkusov á 1740, Praha 9 – Horn í Po č ernice 193 00, Czech Republic – H Đ Tr â` n,
Th e Herbarium, Singapore Botanic Gardens, 1 Cluny Road, 259569 Singapore & University of Science, Vietnam National University, 227 Nguyen Van Cu, Q5, Ho Chi Minh City, Vietnam
Curcuma pygmaea Š korni č k & Š í da f (Zingiberaceae), a new species from Vietnam, is described and illustrated Notes on its closest allies in Curcuma subgenus Hitcheniopsis , C parvifl ora Wall and C thorelii Gagnep., are provided and a lectotype
of C thorelii is designated
Cambodia, Laos and Vietnam are a known diversity hotspot
for various plant families, although detailed data on
Zingib-eraceae are lacking as the last comprehensive account of this
region is over a century old (Gagnepain 1908) Our recent
work, consisting of extensive herbarium studies as well as fi eld
exploration of this region, confi rms the richness of gingers
we suspected and has resulted in descriptions of several new
and often stenoendemic species including two in Curcuma
(Leong- Š korni č kov á et al 2010; Leong- Š korni č kov á & L ý
2010) All three currently accepted subgenera of Curcuma ,
Curcuma, Ecomata and Hitcheniopsis , are well represented in
the Indochinese fl oristic region (sensu Takhtajan 1986) but
the identities of several species described in the past remain
unclear and numerous names have yet to be typifi ed As the
fi rst author works towards a revision of Curcuma for the
notes and type material of two closely related species,
C parvifl ora Wall and C thorelii Gagnep., are discussed and
a lectotype of C thorelii is designated
Curcuma pygmaea Š korni cˇ k & Š í da f sp
nov (Fig 1)
Similar to Curcuma thorelii Gagnep., but smaller in habit,
up to 20 cm tall (vs up to 50 cm tall), infl orescence with
6-13 green bracts decreasing in size towards the apex and no
coma (vs 15-40 bracts and white spreading coma bracts)
H ’ Leo village, 308 m a.s.l.; 17 July 2010, Tran et al 239
(holotype: SING (incl spirit), isotypes: PR, VNM)
Rhizomatous herb to 20 cm tall Rhizome sparsely branched, main rhizome ovoid, 1.5 – 2 ⫻ ca 1.5 cm, exter-nally light brown, interexter-nally cream white, root tubers
glabrous, leafl ess sheaths and 3 – 6 sheaths of leaves, inner-most as long as the pseudostem, white-green with pinkish tinge towards the base, outer ones gradually decreasing in length, whitish green to white with pink tinge; ligule 2 mm long, bilobed, hyaline, glabrous except for a few stipitate hairs ca 0.2 mm long on the upper margin Leafy shoot with 2 – 5 leaves at fl owering, ultimately to 6 leaves; petiole
3 – 7 cm long (except the fi rst leaves which can be nearly sessile), green, glabrous; lamina elliptic to narrowly ovate,
ca 5.5 – 15 ⫻ 1.5 – 5 cm, glabrous, abaxially dark green with
a red patch along the midrib, adaxially lighter green, margin hyaline, ca 0.1 mm wide, glabrous, apex acuminate, base oblique Infl orescence central Peduncle ca 5 – 10 cm long, hidden between the leaf sheaths Spike 4 – 5 ⫻ ca 2 – 3 cm, coma absent Bracts 6 – 13 per infl orescence, lower bracts
ca 2.2 ⫻ 2.6 cm, completely green, glabrous, connate to one another in lower 2/3, strongly refl exed at apex, all except the uppermost 1 – 2 fertile (these are smaller, ca 1.5 ⫻ 1.2 cm) Cincinni with 6 fl owers at the base of the infl orescence, the number gradually decreasing upwards Bracteoles minute, one per fl ower, triangular, 5 – 7 ⫻ 3 – 4 mm at base, hyaline, translucent-white Flowers ca 3 cm long, slightly exserted from bracts Calyx 6 – 7 mm long, 3-toothed, unilaterally split for 2 – 3 mm from apex, white, glabrous Floral tube
ca 1.8 cm long, narrowly cylindrical and funnel-shaped towards the top, externally white, glabrous, internally white, hairy in apical part; dorsal corolla lobe triangular-ovate,
ca 6 mm long and 4 mm at base, concave, glabrous, white,
Nordic Journal of Botany 31: 639–647, 2013
doi: 10.1111/j.1756-1051.2012.01749.x,
© 2013 Th e Authors Nordic Journal of Botany © 2013 Nordic Society Oikos
Subject Editor: John Parnell Accepted 30 October 2012
Trang 2Figure 1 Curcuma pygmaea Š korni č k & Š í da f sp nov (A) habit and detail of anther (inset), (B) fl ower (front view), (C) fl ower (side view),
(D) fl ower dissection and bract Based on living collection Tran et al 239; photos by Jana Leong- Š korni č kov á
Trang 3apex slightly mucronate, with mucro ca 0.3 mm long,
gla-brous; lateral corolla lobes 5.5 ⫻ 3 mm, triangular-ovate,
apex rounded, slightly concave, glabrous, white Lateral
sta-minodes ovate-rhomboid with asymmetric base, 6 – 7 ⫻ 4 – 5
mm, white turning light violet towards the apex Labellum
split ca 3 mm, basal two thirds of the labellum violet with
white and dark violet patches in the centre, apex light violet
Anther spurless, 2.75 ⫻ 1.5 mm, light violet, with glandular
hairs on the sides of the connective, anther thecae 1.5 mm
long, cream white, dehiscing along the entire length; fi
la-ment 1 – 1.5 mm long, white tinged violet, ca 2 mm broad
at base, ca 1 mm at the top, glabrous Anther crest present,
funnel-shaped, ostiole glabrous Epigynous glands absent
gla-brous Fruits and seeds not seen Description based on
her-barium, living and spirit material (Tran et al 239)
Note: In cultivation, Curcuma pygmaea may become
veg-etatively more robust, with laminas to 25 ⫻ 9 cm, and infl
o-rescences with up to 16 bracts, but the sizes of the fl ower
parts do not change
Phenology, habitat and distribution
Curcuma pygmaea occurs in dipterocarp forests and fl owers
are either plain green or with a red patch and this varies within
a single population So far, this species is known only from
the type locality and no specimens have been encountered
during extensive revision of herbarium material at AAU, BK,
BKF, CAL, CMU, E, HN, HNIP, HNU, K, L, P, QBG, and
ai-land we have noticed several specimens (morphotypes 4 and
5) which also seem to lack a white coma and have fewer bracts
than C parvifl ora , but they are of more slender habit than
C pygmaea , and the information regarding the fl ower colour
specimens may represent yet other new taxa, but further examination of fl owering material is needed
Chromosome count and genome size
PI-fl ow cytometry on Partec CyFlow ML following Otto ‘ s
size has been estimated as 2C ⫽ 2.152 pg (measured against internal standard Bellis perennis L., 2C ⫽ 3.42 pg) Th e
prepared by the rapid squash method following the metho-dology described in Leong- Š korni č kov á et al 2007)
Etymology, vernacular names and uses
( ⫽ dwarfi sh) and refl ects the small habit of this plant So far no vernacular names or uses have been recorded for this species
Similar species
Curcuma pygmaea falls in Curcuma subgenus Hitcheniopsis ,
both according to Schumann ’ s (1904), and the most recent infrageneric classifi cation by Z á vesk á et al (2012) Th is sub-genus is characterized by a central infl orescence with obtuse bracts, adnate laterally and recurved at the tips, by the lack of anther spurs and, as pointed out by Leong- Š korni č kov á et al (2007), most importantly, by the lack of epigynous glands
Th e closest species to C pygmaea are C parvifl ora Wall and
C thorelii Gagnep (Fig 2) Unlike C pygmaea , both these
species have a clear distinction between green fertile bracts and white coma bracts
Figure 2 Curcuma parvifl ora and Curcuma thorelii (A) Curcuma parvifl ora, Th ailand, from living collections of Singapore Botanic Gardens Acc
No 20020340, photo by Jana Leong- Š korni č kov á (B) Curcuma thorelii, S Vietnam, based on Tran et al 178, photo by Tr â` n H u~ u Đ ă ng
Trang 4and fi eld records indicate that it occurs in Cambodia, Laos
simi-larly coloured with the labellum and lateral staminodes
being violet, but plants of C thorelii are much larger and more robust than C pygmaea , the infl orescence has
well-developed white coma bracts and the lateral staminodes and labellum are usually of a deeper colour
Typifi cation
1830 – Holotype (implicit): [icon] “ Curcuma parvifl ora ” in
Wallich, Pl Asiat Rar t 57 1830
Unlike several other ginger species described by Wallich, where a specimen and a colour plate form the original mate-rial, the colour plate published with the original description represents the only original element and is therefore treated here as an implicit holotype
Curcuma thorelli Gagnep Bull Soc Bot France 54: 406
1907 – Lectotype (designated here): [Cambodia]
P032708 and sheet with no barcode P!, sheet with no
barcode K000255367)
Curcuma parvifl ora is a species originally described from
forests on the banks of the river Irawaddy, at Prome and also
towards Ava, Burma (Wallich 1830) It is also widespread
from South Vietnam, no specimen was cited and so far we
have not encountered this species in Cambodia, Laos or
characters of C parvifl ora include a small habit, petiolate
leaves, green fertile bracts enclosing cincinni of white fl owers
with a violet labellum and white coma bracts An
examina-tion of herbarium material from the major herbaria listed
above revealed that there is a wide array of morphological
variability across the distribution area with at least fi ve
mor-phological types (Fig 3) Various chromosome counts have
been also reported for this species, namely 2n ⫽ 28, 30, 32,
34, 36 & 42 (Leong- Š korni č kov á et al 2007) suggesting that
C parvifl ora as now recognized is a species with complex
reproductive biology perhaps consisting of more than one
taxon It may also be found to hybridize with other
sympat-rically growing members of Curcuma subg Hitcheniopsis – a
feature we have frequently observed in the fi eld Detailed
studies of living fl owering material of the whole complex,
including cytology, and molecular analyses of these
morpho-types are needed to resolve the complex
locality which at the time was part of Laos (Gagnepain
1907), but is now in Cambodia Our herbarium study
Figure 3 Distribution of Curcuma parvifl ora, C thorelii and C pygmaea based on study of herbarium material Curcuma parvifl ora - 5 major
morphotypes (typical form dark orange, morphotype 2 light orange, morphotype 3 yellow, morphotype 4 light yellow and morphotype 5
white); C thorelii green; C pygmaea in purple Type localities of the three species are marked with stars
Trang 5T Sorensen, K Larsen & B Hansen 3912 (C); ibidem; 9 Jul 1992, P Palee 55 (E, P); ibidem, 8 Jul 1921, A.F.G Kerr 5641 (BK, C); ibidem, 3 Jul 1993, A Phuakan 35 (BKF); ibidem, 11 Jul 1987, J.F Maxwell 87-647 (BKF, L); ibidem, 18 Sep 1967, T Shimizu, H Koyama & M Hutoh T-10467 (AAU); ibidem, 16 Jul 1988, M.N.Tamura T-60072 (BKF); ibidem, 1 Aug 1922, Lung Ai s.n (SN 256845) (BK); near Fang, 25 May 1958, K Larsen &
B Hansen 3598 (E, C); ibidem, 7 Jun 1973, R Geesink,
D Phanichapol & T Santisuk (AAU, BKF, C, E, L, P) Dist Jawm Tong, Mae Soi valley, 29 Sep 1991, J.F Maxwell 91-782 (P, L, E); Mae Sa valley, 9 Jul 1974 J.N Jackson
6134 (BKF); Mai Muang Mao Arboretum, 16 Oct 2001,
W Sankamethawee 259 (L); San Pan See, 18 Jun 2002,
C Glamwaewwong 211 (QBG); along highway between
Iwat-suki & N Fukuoka T-10289 (AAU); en route from Mae Klang waterfall to Sop Aep, 1 Oct 1971, G Murata, K Iwatsuki, C Pengklai & C Charamphoi T-15519 (BKF) Prov Kanchanaburi: Hin Dat, 29 Jun 1926, Put 44 (BK, C); between Huay Ban Kao and Kritee, 2 Jul 1973,
R Geesink & C Phengklai 6070 (AAU, BKF, C, L); ibi-dem, 3 Jul 1973, Maxwell 73-70 (AAU, BK); Ni Kuhn Streamlet, MKWRS, Tong Pha Phum, 12 Mar 1961,
et al 27 (BKF); Brangkasi, 19 – 22 Jun 1946, G Den Hoed &
A Kostermans 478 (BK, L, P); near Wangka, 23 May 1946,
S Bloembergen 633 (BK, L, P); Ta Salao, 12 Jul 1940, A.F.G Kerr 14528 (BK, C, L); ibidem, 11 Jul 1930, A Marcan 2507 (C); Lum Sum, 30 Jul 1928, A Marcan 2339 (C); Sangkha Buri, 12 Mar 1961, K Bunchuai 73 (BKF); Si Sawat, 17 Jul 1963, P.S 2113 (BKF); ibidem, 12 Aug 1971,
CP, BS & BN 2913 (BKF); Sai Yok, 4 Dec 1961, K Larsen
8495 (C); ibidem 9 Jul 1945, A Kostermans 1040 (L); Ban Kao, 10 Nov 1961, K Larsen 8125 (C); Sriswasdi, 2
K Larsen 10539 (AAU); Doi Inthanon, along Mae Klang river, 3 Aug 1988, M.N.Tamura T-60377 (BKF) Prov Lampang: Muang Bahn (Pan) district, Jae Sawn National Park, near Jae Sawn Falls and Mae Nawn Stream, 24 Aug
1995, J.F Maxwell 95-596 (BKF, CMU, L); Distr Muang Pan, Jaesorn National Park, 28 Jun 2002, N
Toyokuni, H Koyama, T Yahara & T Santisuk T-13538 (BKF); Doi Pang La, Huay Tak, 25 Sep 1967, T Shimizu,
H Koyama & A Nalampoon T-10776 (AAU, BKF); Hui Tak, 9 August 1964, C Hambananda 225 (BKF) Prov Mae Hong Son: 19 – 24 km east of Mae Sariang towards the Salawin River, 17 Jul 1998, W.J Kress, M Bordelon &
M Collins 98-6216 (AAU); Along the road between Mae Sariang and Mae La Noi, 18 Sep 1967, K Iwatsuki & N Fukuoka T-10389; ibidem, 28 Jul 2007, A Keratikorkol
352 (QBG); Maesarieng, 8 July 1966, Prayad 345 (BK) Prov Nakhon Sawan: Hua Wai, Taklee, 10 Jul 2007, C Maknoi 1634 (QBG) Prov Phetchaburi: Amphoe Kaeng
Davies & C Hemrat 856 (AAU); ibidem, limestone area in the central part of the Park, Khao Pa Karang, 3 Aug 1995,
to a plant in Curcuma subgenus Hitcheniopsis with green
fertile bracts, white coma bracts and fl owers with
vio-let labellum and lateral staminodes It was described by
Gagnepain (1907), who worked predominantly with
her-barium material supplied to Paris by various collectors
col-lection No 2132 from Stung-treng (currently located in
Cambodia) and specifi cally mentions the blue colour of the
fl owers which is found only in members of subg
Hitch-eniopsis In most other features, it also matches the species
to which the name is currently applied fairly well
Curi-ously, the presence of two anther spurs and two epigynous
glands is also mentioned – both these characters are typical
of Curcuma subg Curcuma but have never been recorded
in subg Hitcheniopsis Our examination of the original
sheets which have to be regarded as syntypes but which
are a mixed collection Plants mounted on three sheets
(barcodes P 032707, P032708 and a sheet without
bar-code) are of a species in subgenus Hitcheniopsis to which
the name C thorelii is currently applied (Fig 4.), while
the plant on the fourth sheet (barcode P 032706) belongs
to Curcuma aff plicata , which is a member of subgenus
Curcuma On the specimen, it may clearly be seen that
Gagnepain sampled and dissected the fl ower of Curcuma
epigynous glands and anther spurs (Fig 5) We have located
represents C thorelii , while the plant on the duplicate at K
is Curcuma aff plicata In order to retain the name of the
species to which it is currently applied and for which there
is no other name available, we designate sheet P 032707
as the lectotype, sheets P032708, another at P without a
barcode, and the specimen at CAL as isolectotypes We
propose here to exclude sheet P 032706 (P!) and sheet
K000255367 (K!) from the original material of C thorelii
Specimens examined:
Curcuma parvifl ora Wall., typical form
Prov Chiang Mai: 30 km S of Bo Luang along the Om
Koi trail, 3 Jul 1968, K Larsen, T Santisuk & E Warncke
2022 (AAU, BKF, E, P, C, L); N of Ban Om Koi, Bo
Luang Tableland, 17 Oct 1979, T Shimizu, H Toyokuni,
BKF, C, L, P); Bo Luang to Om Koi, 10 Sep 1999,
P Srisanga & C Puff 1021 (QBG); ibidem, 12 Jun 1968,
C.F van Beusekom & C Phengklai 1176 (BKF, L); Om
Koi, 20 Jul 1987, C Phengklai et al 6260 (BKF); ibidem,
20 Jul 1987, C Phengklai et al 6302 (BKF); ibidem, 28
Jun 1978, C Phengklai, M Tamura, C Niyomdham &
B Sangkachand 4162 (BKF); ibidem, 28 Jun 1978,
C Phengklai, M Tamura, C Niyomdham & B Sangkachand
4171 (BKF); ibidem, 18 Oct 1979, T Shimizu, H Toyokuni,
H Koyama, T Yahara & T Santisuk T-19354 (BKF); Sri
Sangwan waterfalls, Chiang Dao, 21 Jul 2003, C Maknoi
(QBG); Doi Chiang Dao, 6 Jun 1957, Khontchai 565 (C);
25 Sep 1971, J.E Vidal 5149 (P); Doi Suthep, 5 Jul 1958,
Trang 6km west of Tak, 23 Jul 1973, G Murata, N Fukoka &
C Phengklai T-16873 (AAU, BKF, C, L); km 27 Um Pang-Palata, Um Pang, 1 Jun 2006, S Watthana 1971 (QBG); Lan Sang National Park, 20 Apr 1985, C Niyo-mdham 900 (AAU, BKF, C); ibidem, 1 Sep 1967, M Tagawa, K Iwatsuki, H Koyama & A Chintayungkun T-8662 (AAU); ibidem, 1 Sep 1967, M Tagawa, K Iwat-suki, H Koyama & A Chintayungkun T-8663 (AAU); Amper Mae Sod, 8 Aug 1989, Y Paisooksantivatana 2394-89 (BK)
K Larsen, S.S Larsen, C Tange, R Moran & P Puudjaa
45379 (AAU) Prov Prachuap Khiri Khan: Amphoe Pran
Buri, Kui Buri National Park, trail from park
headquar-ters, 20 Aug 2002, D.J Middleton, S Suddee & C
Hem-rat 1242 (BKF, CMU, E) Prov Ratchaburi: Ban Saphan,
13 Aug 1966, K Larsen, T Smitinand & E Warncke
1547 (BKF); South of Prachuap, 13 Aug 1966, K
Lar-sen, T Smitinand & E Warncke 1557 (BKF); Amper
Suan Pueng, 28 May 1987, Y Paisooksantivatana & P
Sangkhachand 2008-87 Prov Tak: Khao Pha wo, ca 70
Figure 4 Proposed lectotype of Curcuma thorelii , Th orel 2132, P 032707 (A) entire sheet , (B-C) details of infl orescences, (D) detail of
label Photo by Jana Leong- Š korni č kov á
Trang 7Figure 5 A sheet of Curcuma aff plicata proposed to be excluded from the original material of Curcuma thorelii , Th orel 2132, P 032706
(A) entire sheet , (B) detail of infl orescence, (C) drawing of the dissected fl ower attached to the sheet (D) dissection of the fl ower (E) detail
of label Photo by Jana Leong- Š korni č kov á
Trang 8Curcuma parvifl ora Wall., morphotype 5
Th ailand: Prov Saraburi: Muak Lek, hill W of Forest
H Sleumer 1308 (BKF, L), ibidem, 31 Aug 1924, A F G Kerr 9087 (BK, C, L) Muang Dt., Sahm Lahn forest, 12 Jul
1975, J.F Maxwell 75-683 (AAU, BK, L)
Curcuma thorelii Gagnep
Th ailand: Prov Loei: Phu Rua Dt., Phu Rua N P., 12 Jun
2004, T Wongprasert et al 046-65 (BKF) Prov Kalasin: Kuchinarai, Galasintu, 9 Aug 1963, Pradit 355 (BK) Prov
C Maknoi 485 (AAU, QBG) Prov Nong Khai: Dong Srichompoo, 28 Jun 1963, Adisai 577 (BK) Prov Phit-sanulok: Chattrakan Dt., 19 Jun 2006, A.D Poulsen &
P Suksathan 2393 (QBG) Tung Salaeng Luang, 21 Jul
1966, K Larsen, T Smitinand & E Warncke 656 (AAU, BKF, L, P) Prov Sakhon Nakhon: Phu Paan National Park,
6 Aug 2004, I.C Nielsen, T Jonganurak, N Hemrath &
J Rithipheth 1539 (AAU, BKF); ibidem, Aug 1987,
P Yuktathat 142 (E); ibidem, 31 Jul 1999, M.F Newman
945 (BKF, E) Prov Ubon Ratchathani: Phibulmangsa-hawn Dt., 9 Jul 2004, T Wongprasert 047-7 (BKF) Road-side KM 38-37, Ban Sanam Chai Phibun Mungsahan, 21 Aug 2001, R Pooma, W.J.J.O de Wilde, B.E.E Duyfj es,
V Chamchumroon & K Phattarahirankanok 2192 (BKF)
J.E Vidal 1898 (P) Cambodia: Prov Stung Treng: Stung
Klong, 18 Nov 1938, M Muller 517 (P) Vietnam: Prov
Prov T â y Ninh: Tay Ninh, 12 Jul 1939, R Muller 1523 (P) Prov Th u` a Th i ê n-Hu ê´ : Hu ê´ , Sep 1877, Harmand 4891 (P) Prov Dak Lak: Ea H ’ Leo Dist., Ea H ’ Leo village, 17 Jul 2010, Tran et al 238 (PR, SING)
Acknowledgements – We thank the curators of AAU, BK, BKF,
CAL, CMU, E, HN, HNIP, HNU, K, L, P, QBG, SING and VNM herbaria for letting us examine and photograph the speci-mens in their care, the Asian Zingiberaceae Information Centre
at Singapore Botanic Gardens and the Zingiberaceae Resource Centre at the Royal Botanic Garden Edinburgh ( ⬍ http://elmer rbge.org.uk/ZRC/ ⬎ ) for providing protologues and related ref-erences We thank Dr Axel D Poulsen and Dr Mark Newman for useful comments on the manuscript Our research was funded by National Parks Board, Singapore, the Czech Science Foundation, GA Č R (grant no 521/09/0202 and P506/10/0623) and Ministry of Culture of the Czech Republic (DKRVO 2013/10 National Museum, Prague)
References
Gagnepain, F 1907 Zingib é rac é es, Marantac é es et Musac é es nou-velles de l ’ herbier du Museum Bull Soc Bot France 54:
403 – 413
Gagnepain, F 1908 Zingib é rac é es In: Lecomte, H (ed.), Flore G é n é rale de l ’ Indo-Chine Vol 6 Masson and Co,
pp 25 – 121
Leong- Š korni č kov á , J., Š í da, O., Jarol í mov á , V., Sabu, M., F é r, T.,
Tr á vn í č ek, P and Suda, J 2007 Chromosome numbers and genome size variation in Indian species of Curcuma
(Zingiberaceae) Ann Bot 100: 505 – 526
Curcuma parvifl ora Wall., morphotype 2
Th ailand: Prov Chaiyaphum: Ban Nam Phrom, 24 May
1974, R Geesink, T Hattink & C Phengklai 6904 (BKF, L)
NW of Phu Khieo, 6 – 7 Aug 1972, K Larsen, S S Larsen,
I Nielsen & T Santisuk 31551 (AAU) Phu Khieo E of
Chaiyaphum, 3 Aug 1972, K Larsen, S S Larsen, I Nielsen
& T Santisuk 31551 (AAU) Kaengkhro, 18 May 1996, sine
coll., SN113583/BKF118377 (BKF) Prov Chantaburi:
Pong Nam Ron, Hindat, 30 Aug 1956, T Smitinand 3456
(AAU, BKF, C) Foot of Khao Soi Dao, 10 Nov 1969, C.F
van Beusekom & T Smitinand 2097 (AAU, BKF, L, P),
ibidem, 6 Jun 1963, K Larsen 9802 (AAU) Khao Sip-ha
Chan NP, Khun Song, Kaeng Hang maeo, 26 Aug 2004,
K Kertsawang 390 (QBG) Prov Nakhon Nayok: Kow Yai
National Park, Hayosuwat Falls, 31 Aug 1974, J.F Maxwell
74-865 (AAU, BK, L) Prov Nakhon Ratchasima: Kao Yai
National Park, 19 Jul 1973, G Murata, N Fukuoka & C
Phengklai T-16321 (AAU, BKF, C, L); ibidem, 19 Jul 1973,
G Murata, N Fukuoka & C Phengklai T-16372 (BKF);
ibidem, 17 Dec 1962, C Phengklai 567 (c) Khao Ko pang
mar, Paktongchai, 17 Jul 2008, P Weesumritt 238 (QBG)
Prov Prachin Buri: Khao Yai National Park, Khao Khieo,
Heo Suwat Falls, 8 Jul 1967, K Larsen, T Smitinand &
E Warncke 94 (AAU, BKF, P)
Curcuma parvifl ora Wall., morphotype 3
Th ailand: Prov Kamphaeng Phet: Mae Wong National Park
– Kamphaeng Phet side, near HQ, 7 Jul 1999, C
Ngambria-sakul 32 (BKF, E) Klong Laan Waterfall, Nam Tok Klong
Laan, Klong Laan, 25 Oct 2001, S Watthana 1509 (QBG)
Klong Lan National Park, track to east of Waterfall, 21 Aug
1995, Parnell, Pendry, Jebb & Pooma 95-319 (AAU) Klong
Lan, 1 km from Parks HQ 27 Jul 1997, M Van den Bult 13
(BKF) Wang Chao Waterfalls, 4 Oct 2003, C Maknoi 522
(QBG) Prov Loei: Phu Krading, 11 Sep 1963, H Sleumer
4753 (L), ibidem, 3 Sep 1967, T Schimizu, M Hutoh &
D Chaiglom 8779 (AAU, BKF), ibidem, 5 Sep 1969, P
Sanhkhachand 2072 (BK), ibidem, 15 Oct 1967, Preyad
1035 (BK) Wang Sa Pung, 21 Apr 1948, D Nakkan 215
(BKF) Prov Lop Buri: Huai Hin, Chaibadan, 11 Jul 2007,
C Maknoi 1642 (QBG) Prov Petchabun: Ban Nam Ron,
Mueang, 19 Jul 2002, C Maknoi 299 (QBG) Haeo Sai
Waterfall, Namnao NP, 7 Jul 2002, C Maknoi 268 (QBG)
Prov Phitsanulok: Kaeng Sopa Waterfalls, Nakornthai, 24
Luang National Park, about 20 km E of Phitsanulok, 21 Oct
1984, G Murata, C Phengklai, S Mitsuta, T Yahara,
Salaeng Luang National Park, Tan Ta Wang village, 22
Oct 1984, G Murata, C Phengklai, S Mitsuta, T Yahara,
H Nagamasu & N Nantasan T-38217 (BKF)
Curcuma parvifl ora Wall., morphotype 4
Th ailand: Prov Nong Khai: Phu Wua Wildlife sanctuary,
9 Aug 2004, I.C Nielsen, C Niyomdham, T Jonganurak,
Seka district, Jet Si Waterfalls, 20 May 2004, R Pooma,
4109 (BKF)
Trang 9Leong- Š korni č kov á , J., Tr â `n, H.D and Newman, M.F 2010
Curcuma vitellina (Zingiberaceae), a new species from
Vietnam Gard Bull Singapore 62(1): 111 – 117
Leong- Š korni č kov á , J and L ý , N.S 2010 Curcuma pambrosima
sp nov (Zingiberaceae) from central Vietnam Nord J Bot
28: 652 – 655
Otto, F 1990 DAPI staining of fi xed cells for high-resolution fl ow
cytometry of nuclear DNA – In: Crissman H.A.,
Darzynkie-wicz, Z (eds.), Methods in cell biology, Vol 33 New York:
Academic Press, pp 105 – 110
Ph a m, H.H 2003 C â y c ỏ Vi ê t Nam, An illustrated Flora of
Vietnam Vol 3: 432 – 461 Youth Publication, Hochiminh City
Schumann, K 1904 Zingiberaceae – In: Engler, A (ed.), Das
Pfl anzenreich IV, Vol 46 Leipzig, pp 1 – 458
Takhtajan, A 1986 Floristic regions of the World, (trans T.J Crovello & A Cronquist) Berkeley: University of California Press
Wallich, N 1830 Plantae Asiaticae Rariores, vol 1, part 3 – Treuttel and W ü rtz: Treuttel Junr and Richter London, England
Z á vesk á , E., F é r, T., Š í da, O., Krak, K., Marhold, K and
Š korni č kov á , J 2012 Phylogeny of Curcuma (Zingiberaceae)
based on plastid and nuclear sequences: Proposal of the new
subgenus Ecomata Taxon 61 (4): 747 – 763