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DSpace at VNU: Kinetics and mechanistic aspects of As(III) oxidation by aqueous chlorine, chloramines, and ozone: Relevance to drinking water treatment

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Rate constants for the reactions of AsIII with FAC, NH2Cl, NHCl2, and O3, were measured by various techniques selected according to reactant characteristics and reaction rates.. The tren

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Kinetics and Mechanistic Aspects

of As(III) Oxidation by Aqueous

Chlorine, Chloramines, and Ozone:

Relevance to Drinking Water

Treatment

M I C H A E L C D O D D ,† N G O C D U Y V U ,‡

A D R I A N A M M A N N ,† V A N C H I E U L E ,‡

R E I N H A R D K I S S N E R ,§

H U N G V I E T P H A M ,‡ T H E H A C A O ,‡

M I C H A E L B E R G ,† A N D

U R S V O N G U N T E N *, †

Swiss Federal Institute of Aquatic Science and Technology

(EAWAG), 8600 Duebendorf, Switzerland, Center for

Environmental Technology and Sustainable Development

(CETASD), Hanoi University of Science, Nguyen Trai Street

334, Hanoi, Vietnam, and Laboratory of Inorganic Chemistry,

ETH Zurich, 8093 Zurich, Switzerland

Kinetics and mechanisms of As(III) oxidation by free

available chlorine (FACsthe sum of HOCl and OCl-),

ozone (O3), and monochloramine (NH2Cl) were investigated

in buffered reagent solutions Each reaction was found

to be first order in oxidant and in As(III), with 1:1 stoichiometry.

FAC-As(III) and O3-As(III) reactions were extremely

fast, with pH-dependent, apparent second-order rate

constants, k ′′app, of 2.6 ((0.1) × 105M-1s-1and 1.5 ((0.1)

× 106M-1s-1at pH 7, whereas the NH2Cl-As(III)

reaction was relatively slow (k ′′app) 4.3 ((1.7) × 10-1

M-1s-1at pH 7) Experiments conducted in real water

samples spiked with 50 µg/L As(III) (6.7 × 10-7M) showed

that a 0.1 mg/L Cl2(1.4 × 10-6M) dose as FAC was

sufficient to achieve depletion of As(III) to <1 µg/L As(III)

within 10 s of oxidant addition to waters containing

negligible NH3 concentrations and DOC concentrations

<2 mg-C/L Even in a water containing 1 mg-N/L (7.1 × 10-5

M) as NH3, >75% As(III) oxidation could be achieved

within 10 s of dosing 1-2 mg/L Cl2(1.4-2.8 × 10-5M) as

FAC As(III) residuals remaining in NH3-containing waters

10 s after dosing FAC were slowly oxidized (t1/2g 4 h) in the

presence of NH2Cl formed by the FAC-NH3reaction.

Ozonation was sufficient to yield >99% depletion of 50

µg/L As(III) within 10 s of dosing 0.25 mg/L O3(5.2 × 10-6

M) to real waters containing <2 mg-C/L of DOC, while

0.8 mg/L O3(1.7 × 10-5M) was sufficient for a water containing

5.4 mg-C/L of DOC NH3had negligible effect on the

efficiency of As(III) oxidation by O3, due to the slow kinetics

of the O3-NH3reaction at circumneutral pH

Time-resolved measurements of As(III) loss during chlorination

and ozonation of real waters were accurately modeled using the rate constants determined in this investigation.

Introduction

Arsenic is a common contaminant of groundwater resources

around the world (1-4) Soluble inorganic arsenic occurs in

surface waters and groundwaters primarily as a combination

of arsenous acid (As(III)) and arsenic acid (As(V)) (1) The

former state predominates under anoxic conditions (e.g., in oxygen-limited groundwaters), and the latter under oxic

conditions (1), although As(III) can exist as a meta-stable

species even in oxygen-rich environments, due to the slow

kinetics of its oxidation by oxygen (1) Generally, dissolved arsenic occurs in groundwaters at concentrations <5 µg/L (1, 2, 5) However, in certain regions, including the western United States (2, 6) and southern Asia (3, 4), groundwaters

utilized for drinking water often contain arsenic

concentra-tions in substantial excess of the 10 µg/L guideline value recommended by the WHO (5) and adopted as a regulatory limit by the EU (7) and USEPA (8).

When sufficient infrastructure is available, aqueous

arsenic concentrations can be lowered to e 10 µg/L by a

variety of conventional drinking water treatment methods, though many of these methods remove As(III) substantially

less efficiently than As(V) (9) In cases for which As(total) is

constituted in large part by As(III), arsenic removal by such methods can be improved by preoxidizing As(III) to As(V)

(9-11) However, oxidant-scavenging matrix constituents,

such as dissolved organic matter (DOM) and NH3- which can be present at high concentrations within reduced,

As-(III)-laden groundwaters (3, 12, 13), may impair As(III)

oxi-dation efficiency by competing with As(III) for available

oxidant (10) Quantitative knowledge of the rate constants

and mechanisms governing oxidation of As(III) by common drinking water oxidants would greatly facilitate modeling and optimization of As(III) oxidation processes for treatment

of such waters

Apparent second-order rate constants, k′′app, were mea-sured for oxidation of As(III) by FAC, NH2Cl, and O3within the pH range 2 to 11, to permit evaluation of pH-dependencies for each reaction Stoichiometries and reaction orders were also measured, to facilitate identification of probable oxida-tion mechanisms Addioxida-tional experiments were conducted

in water samples collected from Lake Zurich in Switzerland, and from two groundwater treatment facilities in Hanoi, Vietnam, to quantify the effects of matrix composition on As(III) oxidation efficiency and to test the suitability of measured rate constants for modeling As(III) oxidation in real water systems

Materials and Methods

Chemical Reagents As(III) and As(V) stock solutions were

prepared from NaAsO2(purity g99%) and Na2HAsO4‚7H2O (purity g98.5%) obtained from Fluka FAC stock solutions were prepared from NaOCl (∼7% available chlorine) obtained from Riedel-de Hae¨n, and standardized by iodometric

titration (14) Chloramine and O3 stocks were prepared

according to published procedures (15, 16) Additional

reagents were commercially available and of at least reagent grade purity All stock solutions were prepared in deionized water (F g 18.2 MΩ-cm) obtained from a Millipore Milli-Q

or Barnstead NANOpure water purifier Fifty-mM As(III) stocks were prepared approximately bi-monthly, during which time they were stable to within 5% of their initial

* Corresponding author phone: +41 44 823 52 70; fax: +41 44 823

52 10; e-mail: vongunten@eawag.ch

†Swiss Federal Institute of Aquatic Science and Technology

(EAWAG)

‡Hanoi University of Science

§Laboratory of Inorganic Chemistry, ETH Zurich

Environ Sci Technol.2006,40,3285-3292

10.1021/es0524999 CCC: $33.50  2006 American Chemical Society VOL 40, NO 10, 2006 / ENVIRONMENTAL SCIENCE & TECHNOLOGY 93285

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concentration Working As(III) solutions were prepared from

these stocks before each experiment Aluminosilicate

ad-sorbent for the separation of As(III) and As(V) (described in

detail in the Supporting Information, Text S1), was purchased

from Dr Xiaoguang Meng, Stevens Institute of Technology,

Hoboken, NJ

Analytical Methods As(III) and As(V) concentrations were

measured by ion-chromatography/ICP-MS (see Supporting

Information, Text S1 for details) p-Chlorobenzoic acid (pCBA)

and benzaldehyde analyses were performed by HPLC-UV

(Text S1)

Determination of Rate Constants Rate constants for the

reactions of As(III) with FAC, NH2Cl, NHCl2, and O3, were

measured by various techniques selected according to

reactant characteristics and reaction rates In each case,

oxidant consumption was measured in the presence of a

large excess of As(III), to maintain pseudo-first-order

condi-tions with respect to oxidant Individual experimental

procedures are summarized in Table 1 and described in detail

within Text S2

Stoichiometric Measurements Stoichiometries of the

reactions between As(III) and each oxidant (excluding NHCl2)

were measured with either As(III) or the oxidant in excess

In each case, increasing concentrations of the limiting

reactant were dosed under constant, rapid stirring to buffered

solutions of the reactant-in-excess Reactions were allowed

to proceed for time intervals sufficient to ensure complete consumption of the limiting reactant, prior to sampling and analysis for the reactant-in-excess When As(III) was dosed

to solutions containing excess O3, experiments were con-ducted under gastight conditions to minimize evaporative

O3losses (details in Text S3)

Real Water Experiments Water samples used for real

water experiments are listed with corresponding water quality parameters in Table 2 These waters, which contained native

As(III) concentrations <2 µg/L, were spiked with 50 µg/L

As(III) for experiments Separate aliquots of Lake Zurich water were spiked with 1 or 20 mg-N/L (7.1× 10-5or 1.4× 10-3 M) as NH3to simulate waters containing high NH3and low DOC concentrations Sample procurement details and source water descriptions are provided in Text S4 Methods utilized for real water experiments are summarized in Table 1, and described in detail within Text S5

Results and Discussion

As(III) Oxidation Kinetics Pseudo-first-order rate constants,

k′obs, were obtained for reactions of As(III) with each oxidant

by linear regression of plots of ln([Oxidant]) versus time, or

by exponential regression of plots of [Oxidant] versus time, where appropriate (details are provided in the Supporting

TABLE 1 Experimental Approaches Used for Rate Constant Measurements and Real Water Experiments

experiment (method) a T (°C) measurement endpoint e experimental matrix(es) f,g

quenching agent(s) g

NH2Cl kinetics (batchb) 25 ((0.5) NH2Cl loss (measured at λ ) 243 nm) buffered As(III) stock NA

NHCl2kinetics (batchb) 25 ((0.5) NHCl2loss (measured at λ ) 310 nm) buffered As(III) stock NA

FAC kinetics (CFLb) 23 ((2)d FAC loss (measured via DPD method) buffered As(III) stock (C1),

buffered FAC stock (C2)

DPD (C3)

O3kinetics (CFLb) 23 ((2)d O3loss (measured via indigo method) buffered As(III) stock (C1),

pH 4 O3stock (C2)

indigo (C3)

O3kinetics (SFLb) 20 ((0.5) O3loss (measured at λ ) 258 nm) buffered As(III) stock (C1),

buffered O3stock (C2)

NA real water chlorination

(batchc)

25 ((0.5) measurement of As(III) loss for various

FAC doses

As(III)-spiked real water ascorbic acid

or DPDi real water chlorination

(batchc)

25 ((0.5) measurement of As(III) loss in the presence

of various NH2Cl concentrations

As(III)-spiked real water ascorbic acid

or DPDi real water ozonation

(batchc)

20 ((0.5) measurement of As(III) loss for various

O3doses

As(III)-spiked real water none real water chlorination

(CFLc)

23 ((2)d time-resolved monitoring of As(III) loss for

an applied excess of FAC

As(III)-spiked real water (C1), buffered FAC stock (C2)

ascorbic acid

or DPD (C3)i real water ozonation

(CFLc)

23 ((2)d time-resolved monitoring of As(III) loss

for an applied excess of O3

As(III)-spiked real water (C1),

pH 4 O3stock (C2a), buffer (C2b)h

cinnamic acid (C3)j

a CFL-continuous-flow, SFL-stopped-flow b Experimental details included in the Supporting Information, Text S2 c Experimental details included

in the Supporting Information, Text S5 d Room temperature e Reaction kinetics experiments conducted with As(III) in large excess of each oxidant.

Real water experiments conducted at starting concentrations of 50 µg/L As(III) (6.7× 10 -7 M) f Phosphate, acetate, and borate buffers adjusted

to desired pH values in reaction kinetics experiments or, in real water experiments, to the appropriate real water’s native pH O 3 stocks acidified

to pH 4 by dropwise addition of 150 mM sulfuric acid g As(III)-containing solutions introduced on channel 1 (C1) of the CFL system, oxidant solutions on channel 2 (C2), and quenching reagent solutions on channel 3 (C3) h O 3 stock (C2a) and buffer (C2b) premixed to yield a buffered

O 3 stock (C2) at the real water pH immediately prior to further mixing with As(III)-spiked real water (C1) i Ascorbic acid used to quench samples intended for As(III) analyses and DPD used to monitor FAC residuals j The O 3 -cinnamic acid reaction yields benzaldehyde in 1:1 stoichiometry (17) Residual O 3 concentrations were calculated from benzaldehyde concentrations in quenched samples.

TABLE 2 Water Sources and Important Parametric Measurementsa

a Multiple samples of each water, collected on different dates over a four-month time-span, were utilized to conduct the real water experiments described herein; thus, ranges of measurements are provided for each water quality parameter Single values indicate that measurements were the same for each sample b Separate aliquots of native LZ water were amended with NH 4 Cl (LZ1 with 1 mg-N/L (7.1 × 10 -5 M), and LZ20 with

20 mg-N/L (1.4 × 10 -3 M)) for use in batch and time-resolved chlorination experiments.

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InformationsPart B) As(III) reaction orders were determined

by evaluating the dependence of k′obson [As(III)] for each

reaction Plots of log(k′obs) v log([As(III)]) for FAC, NH2Cl,

and O3reactions all yielded slopes of 1.0 ((0.07) (Figure S1),

indicating that each reaction can be treated as first-order

with respect to As(III) The reactions of As(III) with FAC,

NH2Cl, and O3could thus be described by a second-order

kinetic model (eq 1),

where k′′app(calculated by dividing k′obsby [As(III)])

repre-sents the pH-dependent, apparent second-order rate

con-stant at a particular pH In contrast to its reactions with the

other oxidants, As(III) was found to react with NHCl2with

an order of 0.7 (Figure S1)

Free Available Chlorine Figure 1a, which shows the

magnitude of k′′app,FACat various pH values, illustrates that

As(III) reacts very rapidly with FAC k′′app,FACis 2.6 ((0.1)×

105M-1s-1at pH 7, corresponding to a t1/2,As(III)of 95 ms in

the presence of 2 mg/L Cl2(2.8× 10-5M) as FAC The

pH-dependency of k′′app,FACcan be attributed to varying

contri-butions of each reactant’s acid-base species to apparent

FAC-As(III) reactivity

As(OH)3 dissociates in aqueous solution according to

eqs 2-4 (18).

At circumneutral pH, As(OH)3represents the most abundant

As(III) species A small fraction (up to∼0.1) of As(III) is present

as As(OH)2O-under these conditions, and very small fractions

(<5× 10-6) are present as As(OH)O22-and AsO33-(Figure

1a) FAC speciation can be described by eq 5 (19).

FAC-As(III) reaction kinetics can be characterized according

to eight possible reactions between the four As(III) species

(eqs 2-4) and two FAC species (eq 5), by incorporating species

distribution terms into eq 1 and rearranging to yield eq 6,

where Ri and β jrepresent the respective fractions of oxidant

and substrate present as the species i and j at a given pH

(20), and k′′ij represents the specific second-order rate

constant for each i and j pair.

The increase in magnitude of k′′app,FACup to pH 8.3 can be

attributed primarily to an increase in the fraction of As(III)

present as As(OH)2O-, which is expected to be a stronger

nucleophile than As(OH)3 The decrease in magnitude of

k′′app,FACabove pH 8.3 can be attributed to an accompanying

decrease in proportion of HOCl relative to OCl-, which is a

much weaker oxidant than HOCl (15, 21-23) Consequently,

the magnitude of k′′app,FACis highest near pH 8.3 (the average

of pKa1,As(III)and pKa,HOCl), where the product RHOClβAs(OH) 2 O

-(R1β2) reaches a maximum (Figure 1a) These observations

indicate that OCl-reactions are unimportant relative to HOCl

reactions within the pH range studied Therefore, the

magnitude of k′′app,FACis governed primarily by the reactions

of HOCl with each acid-base species of As(OH)3 k′′app,FAC can thus be modeled by neglecting OCl-reactions

Specific rate constants, k′′1j - calculated by nonlinear

regression of measured k′′app,FACvalues, according to eq 6 (via SigmaPlot 2002, SPSS software), are summarized in Table 3 The model fit shown in Figure 1a, which was obtained by

using these k′′1jvalues, demonstrates the accuracy of eq 6 in

describing measured magnitudes of k′′app,FAC k′′14could not

be accurately determined from available data However, this term is unimportant within the pH range studied, as

HOCl-As(III) reactivity is governed almost exclusively by k′′11, k′′12,

and k′′13under these conditions (Figure 1a)

Chloramines The magnitude of k′′app,NH2Cl is shown at various pH values in Figure 1b These data illustrate that

d([As(III)])

d([Ox])

dt ) - k′′app[As(III)] [Ox] ) -k′obs[Ox] (1)

k′′app,FAC) ∑

i)1,2

j)1,2,3,4

k′′ijR

i [FAC] β j[As(III)]

[FAC] [As(III)]

i)1,2 j)1,2,3,4

k′′ijR

i β j

(6)

FIGURE 1 Apparent second-order rate constants for As(III) oxidation

by (a) FAC, (b) NH 2 Cl, and (c) O 3 FAC experiments conducted at [As(III)] ) 15-50 × 10 -6 M, [FAC] 0 ) 1.5-5 × 10 -6 M, and 23 ((2)

°C, NH 2 Cl experiments at [As(III)] ) 5 × 10 -3 M, [NH 2 Cl] 0 ) 2 ×

10 -4 M, and 25 ((0.5) °C, and O 3 experiments at [As(III)] ) 10-200 × 10 -6 M, [O 3 ] 0 ) 1-10 × 10 -6 M, and 23 ((2)°C (CFL) or

20 ((0.5)°C (SFL).

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As(III) reacts relatively slowly with NH2Cl k′′app,NH2Cl is 4.3

((1.7)× 10-1M-1s-1at pH 7, corresponding to a t1/2,As(III)of

16 h in the presence of 2 mg/L Cl2(2.8× 10-5M) as NH2Cl

The inverse relationship between pH and magnitude of

k′′app,NH2Clfrom pH 8 to 11 (Figure 1b) is suggestive of

acid-catalysis, in analogy to the reactions of NH2Cl with SO3

2-(24), NO2-(25), and I-(15) This catalysis appears to be H+

-specific, as k′′app,NH2Clexhibited no measurable dependence

on phosphate (50-182 mM) or borate (10-80 mM)

con-centrations

The plateau in magnitude of k′′app,NH2Cl below pH 8

indicates that NH2Cl-As(III) reaction kinetics are not

sig-nificantly influenced by neutral As(OH)3within the pH range

studied, because H+-catalyzed oxidation of As(OH)3would

require that the magnitude of k′′app,NH2Clincrease

continu-ously with increasing acidity The trends in Figure 1b can,

therefore, be attributed to H+-catalyzed reactions of NH2Cl

with one or more anionic As(III) species, according to eq 7,

where k′′′j represents the respective third-order H+

-cata-lysis rate constants for each of the three anionic As(III) species,

j In the context of eq 7, the data in Figure 1b also suggest

that the magnitude of k′′app,NH2Cl is governed primarily by

As(OH)2O- below pH 8 Under these conditions, each

successive unit decrease in pH is offset by an order of

magnitude decrease in the mole fraction of As(OH)2O-,

resulting in a constant value for the product of the [H+] and

β jterms in eq 7 This should, in turn, lead to a constant value

of k′′app,NH2Cl, assuming that As(OH)O22- and AsO33-have

minimal influence on reaction kinetics below pH 8

These inferences were tested by nonlinear regression of

measured k′′app,NH2Clvalues according to eq 7 The resulting

model fit, obtained with the k′′′As(OH)2O -and k′′′As(OH)O22-values

listed in Table 3, is shown in Figure 1b k′′′AsO33-could not be

accurately determined, due to lack of data above pH 11

However, this term is unimportant within the pH range

studied, as the magnitude of k′′app,NH2Clis clearly influenced

primarily by k′′′As(OH)2O -and k′′′As(OH)O22-between pH 6.5 and 11

(Figure 1b)

An Arrhenius plot of k′′app,NH2Clfrom 10 to 30°C showed

that E afor the As(III)-NH2Cl reaction is 27 ((2) kJ/mol

(Supporting Information, Figure S2) A temperature change

of 10°C will, therefore, result in variation of k′′app,NH2Clby a

factor of 1.4-1.5 within temperature ranges relevant to

drinking water treatment

NHCl2-As(III) reaction kinetics were found to be far slower

than NH2Cl-As(III) kinetics k′obs,NHCl2increased from 0.4×

10-5to 2.4× 10-5s-1(i.e., t1/2) 8-48 h) in the presence of

13 mM of As(III), as pH decreased from 4 to 5 (Supporting

Information, Figure S3) These data indicate that the reaction

of As(III) with NHCl2can be neglected under typical drinking

water disinfection conditions

Ozone The magnitude of k′′app,O3, measured by CFL and SFL methods, is shown as a function of pH in Figure 1c As illustrated by these data, As(III) reacts extremely rapidly with

O3 k′′app,O3is 1.5 ((0.1)× 106M-1s-1at pH 7, corresponding

to a t1/2,As(III)of 11 ms in the presence of 2 mg/L O3(4.2× 10-5

M) The magnitude of k′′app,O3is also strongly pH-dependent However, oxidant speciation does not need to be considered for O3reactions, so k′′app,O3can be characterized according to

As(III) speciation alone The constancy of k′′app,O3below pH

6 can be attributed to the O3-As(OH)3reaction, whereas the

increase in k′′app,O3above pH 6 can be attributed primarily to the O3-As(OH)2O-reaction As(OH)O22-and AsO33-exert

negligible influence on the magnitude of k′′app,O3below pH 8.5, since molar fractions of these two species are very small under such conditions (<5× 10-6)

k′′j values were determined for the O3-As(III)

reac-tion by fitting eq 6 to k′′app,O3in the same manner as for the As(III)-FAC reaction (with O3 terms substituted for FAC

terms) The resulting model fit, obtained with the k′′As(OH)3

and k′′As(OH)2O-values listed in Table 3, is shown in Figure 1c

k′′As(OH)O22- and k′′AsO33- could not be accurately determined from available data However, the importance of these terms

is negligible within the pH range studied, as apparent from

the nearly exclusive dependence of k′′app,O3 on k′′As(OH)3 and

k′′As(OH)2O -under these conditions (Figure 1c)

Mechanistic Considerations As mentioned above, the

FAC, NH2Cl, and O3reactions were found to be first-order with respect to As(III) and oxidant (Figure S1) In addition, stoichiometries of As(III) oxidation by FAC, NH2Cl, and O3 were found to be 1:1 for all three reactions; that is, one mole

of As(III) was consumed for each mole of oxidant consumed, whether experiments were conducted with As(III) or oxidant

in excess (Supporting Information, Figure S4) Experiments conducted with As(III) in excess also verified that one mole

of As(V) is produced for every mole of As(III) consumed (Figure S4)

Free Available Chlorine On the basis of reaction order

and stoichiometry, the oxidation of As(OH)3by HOCl, yielding AsO(OH)3, superficially resembles a direct oxygen transfer reaction O-transfer would involve direct nucleophilic sub-stitution by As(III) at the oxygen atom in HOCl, with HCl as

a leaving group However, comparison with FAC reaction systems involving other inorganic nucleophiles (e.g., SO32-,

Br-, I-, CN-(22)) suggests that As(III) oxidation more likely

proceeds via initial Cl+-transfer from HOCl to the As atom, with concomitant loss of OH-(a much more favorable leaving group than HCl), to yield a transient As(III)Cl+intermediate that hydrolyzes to Cl-and As(V) (eqs 8 and 9) This pathway

is expected to apply to HOCl reactions with all four As(III) species

TABLE 3 Specific Rate Constants Determined for Reactions of As(III) with HOCl, NH2Cl, and O3

k′′app(M -1 s -1 ), pH 7 ( 1/2 at 2 mg/L oxidant concentration) b

HOCl As(OH)3 k′′11) 4.3 ((0.8) × 103M-1s-1 2.6 ((0.1)× 105(1/2) 95 ms)

As(OH)2O- k′′12) 5.8 ((0.1) × 107M-1s-1 As(OH)O22- k′′13) 1.4 ((0.1) × 109M-1s-1

NH2Cl As(OH)2O- k2′′′a) 6.9 ((2.7) × 108M-2s-1 4.3 ((1.7)× 10-1(1/2) 16 h)

As(OH)O22- k3′′′a) 8.3 ((7.8) × 1010M-2s-1

O3 As(OH)3 k′′1 ) 5.5 ((0.1) × 105M-1s-1 1.5 ((0.1)× 106(1/2) 11 ms)

As(OH)2O- k′′2 ) 1.5 ((0.1) × 108M-1s-1

a Third-order, H +

-catalysis rate constant b calculated for pseudo-first-order conditions of excess oxidant, assuming 2 mg/L concentrations of

FAC (28 µM), NH2Cl (28 µM), and O3(42 µM).

k′′app,NH2Cl) [H+

j)2,3,4

kHOCl,As(OH)3′′

-(8)

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Monochloramine NH2Cl is known to react with a number

of inorganic nucleophiles (e.g., SO32-, NO2-, I-) by

acid-catalyzed Cl+-transfer, to yield the same chloro-intermediates

produced in corresponding FAC reactions (15, 24, 25) The

order and 1:1 stoichiometry of the NH2Cl-As(III) reaction,

together with the pH-dependence of k′′app,NH2Cl, are

consis-tent with a similar mechanism (eq 10), which should apply

to oxidation of all three anionic As(III) species The

chloro-intermediate formed in eq 10 would hydrolyze in analogy to

eq 9

Ozone O3generally reacts with inorganic nucleophiles

by two-electron processes involving O-transfer from O3to

the nucleophile via a primary ozonide adduct, which

decomposes to yield the oxidized substrate and O2(26,27).

The order and 1:1 stoichiometry of the As(III)-O3reaction

indicate that As(III) is similarly oxidized to As(V) by O-transfer

from O3to the As atom (eqs 11 and 12) The same pathway

is expected to apply to reactions of O3with all four As(III)

species

Oxidation of As(III) in Real Waters ChlorinationsFree

Available Chlorine Reactions Figure 2a depicts measured

As(III) losses at various FAC doses in each of the real waters

listed in Table 2 Fifty µg/L As(III) (6.7× 10-7M) was

de-pleted to <1 µg/L As(III) by as little as 0.1 mg/L Cl2(1.4×

10-6M) as FAC during batch experiments conducted with LZ

and YP waters (Figure 2a) Such high As(III) oxidation

efficiency is consistent with the low DOC concentrations

and lack of NH3in these two waters (Table 2) In contrast,

As(III) oxidation efficiency was markedly suppressed in

LZ1, LZ20, and PV waters (Figure 2a), due to rapid scavenging

of FAC by the NH3 present in the latter three waters

(k′′app,FAC,NH3> 1 × 104M-1s-1between pH 7 and 8 (23)).

Time-resolved As(III) losses during chlorination of LZ,

LZ1, and YP waters were monitored by the CFL system

mentioned in Table 1 Results obtained from these

experi-ments are summarized in Figure 2b FAC residuals were

present during the monitored reaction periods in all three

waters, ensuring rapid As(III) oxidation in each case The

higher rate of As(III) oxidation in LZ water, compared to YP

water, can be attributed to the difference in pH of the two

waters; with k′′app,FAC,As(III)) 6.9 × 105M-1s-1at pH 8 (LZ

water), and 3.6× 105M-1s-1at pH 7.2 (YP water) Comparison

of the results for LZ and LZ1 waters shows that As(III)

oxidation efficiency was moderately impaired in the latter,

due to rapid consumption of FAC by NH3(Figure 2b) These

findings are consistent with the results obtained for batch

experiments with the same waters (Figure 2a)

The As(III) losses shown in Figure 2b can be modeled

with the rate constants reported in Table 3, by compensating

for contemporaneous FAC loss to side-reactions with matrix

constituents in each water FAC losses were modeled

according to pseudo-first-order rate “constants,” k′FAC,matrix,

obtained from plots of ln([FAC]) vs time in each water

As(III) oxidation was in turn modeled by inserting the

pseudo-first-order expression for FAC decay (eq 13) into a separate

expression for As(III) oxidation (eq 14), and integrating to

yield eq 15

The resulting model fits are shown as dotted lines in Figure 2b The close agreement between model predictions and measured data for each real water demonstrates that one can accurately predict oxidation of As(III) by FAC in various real waters if the rate of FAC loss for a given water is known However, in certain cases, one can make predictions of expected As(III) oxidation efficiencies even without directly measuring FAC loss rates For example, FAC reacts with NH3 far more rapidly than with DOM and most other matrix constituents; thus, in systems containing substantial NH3 concentrations (e.g., >0.5 mg-N/L), FAC loss will likely be dominated by FAC-NH3 reaction kinetics In such cases, FAC loss can be predicted by modeling FAC consumption according to the second-order reaction between NH3and FAC As(III) loss can in turn be modeled by substituting a second-order expression for FAC loss (eq 16) into eq 14 and

integrating with respect to t, as described in the Supporting

Information (Text S6), to yield eq 17

Model predictions obtained by eq 17 are compared with measurements from LZ1 water in Figure 2c The model substantially over-predicted As(III) losses with respect to batch measurements This was presumably a consequence

of suboptimal mixing in the batch systems, which would have resulted in disproportionately large consumption of FAC by NH3during FAC dosage, in turn leading to lower As(III) loss than predicted for an ideally mixed system However, model predictions correlated very well with CFL measurements (Figure 2c), consistent with the superior mixing efficiency achieved by the CFL system (i.e., FAC and real water solutions are mixed through a tee in 1:1 proportion during CFL experiments, as described in the Supporting Information, Text S2)

ChlorinationsNH 2 Cl Reactions As(III) loss is expected to

occur within two phases during chlorination of a water containing significant NH3concentrations: (i) initial, rapid oxidation of As(III) by FAC, and (ii) secondary, slow oxidation

of As(III) in the presence of NH2Cl generated by the

FAC-NH3 reaction, if insufficient FAC is added to completely oxidize As(III) during the first phase As(III) losses measured

kCl′ As(OH)3

-(9)

kH′′′ + ,NH2Cl,As(OH)2O

kOOOAs(OH)3′

[As(III)] ) [As(III)]0e(-kapp,FAC′′ ∫0t[FAC]dt) (14) [As(III)] )

[As(III)]0exp(k′′app,FAC[FAC]0

k′FAC,matrix (e(-kFAC,matrix′ t)-1)) (15)

[FAC] )

[FAC]0(1 -[NH3]0

(1 -[NH3]0 [FAC]0)e(([NH3 ] 0 -[FAC] 0)kapp,FAC,NH3′′ t)

(16)

[As(III)] )

[As(III)]0exp(-k′′app,FAC,As(III)[FAC]0(1 -[NH3]0

([NH3]0-[FAC]0)k′′app,FAC,NH

3

×

(ln( e(([NH3 ] 0 -[FAC] 0)kapp,FAC,NH3 ′′ t)

[NH3]0

(([NH 3 ] 0 -[FAC] 0)kapp,FAC,NH3 ′′ t)-1)- ln( 1

[NH3]0 [FAC]0-1(17)) ))

Trang 6

within the latter phase, during chlorination of LZ1, LZ20,

and PV waters, are shown in Figure 2d

With the exception of LZ1 water dosed with 0.1 mg/L Cl2

(1.4× 10-6M) as FAC, NH2Cl concentrations in each water

were in substantial excess of [As(III)], and remained

es-sentially constant during monitored reaction periods in these

waters (i.e., <10% change from [NH2Cl]0, data not shown)

As(III) losses were, therefore, modeled initially by eq 18

However, this model substantially under-predicted the rate

of As(III) loss observed within LZ1, LZ20, and PV waters

(dotted lines in Figure 2d), presumably because it does not

account for effects of the equilibrium between NH2Cl and

HOCl (eq 19) on As(III) oxidation

The importance of eq 19 can be investigated by using the

equilibrium constant, Khyd) 1.5 × 1011M-1(28) to

deter-mine the equilibrium concentration, [HOCl]eq, from known

concentrations of NH3(Table 2) and NH2Cl [FAC]eq(including

both HOCl and OCl-) can then be calculated from [HOCl]eq

and incorporated with k′′app,FACinto eq 18, to yield eq 20, by

which contributions of NH2Cl and HOCl to As(III) loss can

be modeled together

As shown by the solid lines in Figure 2d, eq 20 yielded predictions that are in very good accord with measured As(III) loss in LZ1 and LZ20 waters, illustrating that the

NH2Cl-HOCl equilibrium plays a significant role in governing As(III) loss in the presence of excess NH2Cl However, predictions obtained for PV water by eq 20 still deviated substantially from measured As(III) losses (Figure 2d) The reason for these discrepancies is presently unknown

Ozonation Figure 3a depicts measured As(III) losses for

various O3doses in LZ, YP, and PV waters A dose of only 0.25 mg/L O3(5.2× 10-6M) was sufficient to achieve >99% loss

of 50 µg/L As(III) (6.7 × 10-7 M) in LZ and YP waters

Comparable oxidation of 50 µg/L As(III) was also achieved

in PV water at a relatively low O3 dose (0.8 mg/L O3, or 1.7× 10-5M) (Figure 3a), because O3, in contrast to FAC, reacts very slowly with NH3(k′′app,O3,NH3) 0.2 M-1s-1at pH

7.3 (29)) The observation that more O3than FAC (on a molar basis) is required to achieve comparable oxidation of As(III)

in LZ and YP waters (Figures 2a and 3a) can be attributed

to the higher reactivity of O3toward DOM, which results in comparably more rapid O3loss to side-reactions with water matrix constituents

FIGURE 2 As(III) oxidation during chlorination of real waters spiked with 50 µg/L As(III) (6.7× 10 M) (water quality data in Table 2) (a) As(III) loss 10 s after FAC addition to each real water, in batch at 25 ((0.5)°C, (b) time-resolved As(III) loss within LZ, LZ1, and YP waters for an applied FAC dose of 0.5 mg/L Cl 2 (7.1 × 10 -5 M) at 23 ((2)°C, (c) comparison of As(III) losses measured within LZ1 water,

in batch (25 ((0.5)°C) and by CFL (23 ((2)°C), with As(III) losses predicted for the same water by modeling FAC losses according to the second-order reaction between FAC and NH 3 (d) As(III) losses in LZ1, LZ20, and PV waters, in the presence of NH 2 Cl formed from various FAC doses at 25 ((0.5)°C CC1, CC2, and CC3 represent “combined chlorine” (i.e., NH 2 Cl) concentrations of 0.1, 0.25, and 0.5 mg/L Cl 2 (1.4

× 10 -6 , 3.5 × 10 -6 , and 7.1 × 10 -6 M) for LZ1 water, and 0.5, 1.0, and 1.8 mg/L Cl 2 (7.1 × 10 -6 , 1.4 × 10 -5 , and 2.5 × 10 -5 M) for LZ20 and

PV waters DPD measurements verified that [NH 2 Cl] did not decrease more than 10% during the total reaction times in any of these reaction solutions Symbols in b-d refer to measurements, lines to model predictions.

[As(III)] ) [As(III)]0e(-kapp,NH2Cl′′ [NH2Cl] t) (18)

NH3+ HOCl y\zKhyd

NH2Cl + H2O (19)

[As(III)] ) [As(III)]0e(-(kapp,NH2Cl′′ [NH2Cl]+kapp,FAC ′′ [FAC]eq ) t) (20)

Trang 7

Figure 3b, depicting time-resolved measurements of O3

and As(III) losses in LZ, YP, and PV waters, illustrates that

t1/2,O 3is less than 0.33 s in LZ and YP waters, whereas t1/2,FAC

exceeds 1.8 s for the same waters (Figure 2b)

Molar-equivalent doses of FAC and O3, therefore, resulted in similar

rates of As(III) oxidation within these waters, even though

the magnitude of k′′app,O3exceeds that of k′′app,FACby a factor

of 5-15 at circumneutral pH Figure 3b also shows that O3

loss is more rapid in PV water than in LZ or YP waters, due

to the higher DOC concentration in PV water This is

consistent with the comparably lower efficiency of As(III)

oxidation by O3in PV water (Figure 3a) Furthermore, Figure

3b shows that the rate of As(III) loss is significantly faster in

LZ water than in YP water, and approximately equivalent to

the rate of As(III) loss in PV water, even though the latter was

dosed with four times as much O3 This can be attributed in

part to the higher pH of LZ water; that is, k′′app,O3is 9.4× 106

M-1s-1at pH 8 (LZ water), compared to 2.0× 106M-1s-1

at pH 7.2 (YP water) and 2.4× 106 M-1s-1at pH 7.3 (PV

water)

Hydroxyl radicals (‚OH) - generated by autocatalytic

O3decomposition or by direct reactions of O3with water

matrix constituents (30,31) - also react rapidly with As(III)

(k′′‚OH,As(OH)3) 8.5 ((0.9) × 109M-1s-1(32)) p-Chlorobenzoic

acid (pCBA), which reacts rapidly with ‚OH, but is nonreactive

toward O3, was used as an in situ probe (33) to evaluate the

importance of ‚OH-As(III) reactions during ozonation of

each real water The pCBA losses depicted in Figure 3b show

that ‚OH was generated in measurable yield within each

system However, calculated contributions of ‚OH to observed

As(III) losses were very low (i.e., <5% of total observed loss

for LZ and YP waters, and <10% for PV water, see Supporting

Information, Text S7 for a detailed discussion) Time-resolved

measurements of As(III) losses in these waters were, therefore,

modeled by considering only O3-As(III) reaction kinetics

(via eq 15, with O3terms substituted for FAC terms) The

close agreement of model predictions with experimental data

confirms that As(III) loss was dominated by direct reactions

with O3(Figure 3b)

Implications for As(III) Oxidation during Full-Scale

Drinking Water Treatment As demonstrated here and in

prior work (10), oxidant-scavenging matrix constituents such

as NH3and DOM can lower the efficiency of As(III)

preoxi-dation processes Fe(II), which reacts very rapidly with FAC

and O3at pH e 2 (34, 35), may represent another important

oxidant scavenger in such waters, though FAC-Fe(II) and

O3-Fe(II) reaction kinetics must be measured at

circum-neutral pH to permit quantitative evaluation of its potential influence on chlorination or ozonation processes When oxidant-scavenger concentrations are relatively low, their influence on As(III) oxidation efficiency during chlo-rination or ozonation processes will likely be offset by the extremely fast kinetics of FAC-As(III) and O3-As(III) reac-tions However, high scavenger concentrations may sub-stantially impair As(III) oxidation efficiency (Figures 2a and 3a) Proper selection of oxidants can minimize matrix effects

in the latter case For example, ozonation will generally be preferable to chlorination for oxidation of As(III) in waters containing high NH3concentrations (e.g., PV water), because

O3reacts slowly with NH3 In comparison, chlorination is likely to prove more efficient than ozonation for As(III) oxidation in waters lacking NH3, because FAC typically reacts more slowly than O3with DOM over time-scales relevant to FAC-As(III) reactions (Figures 2b and 3b)

In waters with high oxidant scavenging rates, As(III) oxidation efficiencies will also be highly sensitive to mixing efficiency during oxidant application (Figure 2c) The high sensitivity of FAC-As(III) and O3-As(III) reaction kinetics to

pH (Figure 1) indicates that pH control may also play an important role in As(III) oxidation efficiency Careful attention

to these considerations will facilitate optimization of oxidant dose when As(III) oxidation must be balanced with con-straints such as disinfection byproduct formation

In an optimized chlorination or ozonation process, complete preoxidation of As(III) should generally be achiev-able at oxidant doses for which disinfection byproduct formation will be minimal For example, THM and NDMA formation potentials in YP and PV waters are known to be far below WHO, EU, and USEPA limits at the FAC doses required to achieve full As(III) oxidation within these waters

during the present investigation (13) Bromate formation

during ozonation of these waters is also expected to be low, because YP water contains low Br-concentrations (i.e., <30

µg/L), and PV water contains high NH3concentrations, which will substantially suppress bromate formation by scavenging HOBr generated by reaction of O3with Br-(36).

NH2Cl formed during chlorination of ammoniacal waters will likely only have appreciable effect on As(III) fate in special cases; for example, if source waters undergo limited or no treatment prior to chlorination, and insufficient FAC is added

to directly oxidize As(III) during chlorination Although the direct NH2Cl-As(III) reaction may result in minimal As(III) oxidation after chlorination, indirect NH2Cl-mediated oxida-tion reacoxida-tions can yield substantial As(III) oxidaoxida-tion within

FIGURE 3 As(III) oxidation during ozonation of real waters spiked with 50 µg/L As(III) (6.7× 10 M) (water quality data in Table 2) (a) As(III) loss for various O 3 doses within real waters included in this study, in batch at 25 ((0.5)°C, (b) time-resolved As(III) loss within

LZ water ([O 3 ] 0 ) 0.25 mg/L (5.2 × 10 -6 M), 23 ((2)°C), YP water ([O 3 ] 0 ) 0.25 mg/L (5.2 × 10 -6 M), 23 ((2)°C), and PV water ([O 3 ] 0)

1 mg/L (2.1 × 10 -5 M), 23 ((2)°C) Symbols refer to measurements, lines to model predictions.

Trang 8

such systems over reaction times of several hours (e.g., within

disinfection contact chambers or distribution networks), as

illustrated in Figure 2d

Acknowledgments

M.C.D and N.D.V contributed equally to this work Travel

scholarships and financial support for N.D.V and V.C.L were

obtained from the Swiss Agency for Development and

Cooperation (SDC), in the framework of the Swiss-Vietnamese

project ESTNV (Environmental Science and Technology in

Northern Vietnam) M.C.D gratefully acknowledges financial

support from a U.S National Science Foundation Graduate

Research Fellowship The authors thank Elisabeth Salhi,

Caroline Stengel, and Sebastien Meylan for their technical

assistance Willem Koppenol is acknowledged for support in

obtaining stopped-flow measurements of As(III)-O3reaction

kinetics The authors also thank Stephan Hug, Linda Roberts,

Olivier Leupin, Marc-Olivier Buffle, and Gretchen Onstad

for many helpful discussions The Hanoi Water Works

Company is acknowledged for assistance in obtaining water

samples from Hanoi

Supporting Information Available

Tables and figures addressing experimental methods and

modeling approaches, water sample sources and

procure-ment, reaction orders and stoichiometries, As(III)-NHCl2

reaction kinetics, and temperature-dependence of

As(III)-NH2Cl reaction kinetics, in addition to reaction kinetics data

from which rate constants were determined This material

is available free of charge via the Internet at http://

pubs.acs.org

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Received for review December 13, 2005 Revised manuscript received March 7, 2006 Accepted March 10, 2006.

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