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DSpace at VNU: Syntheses and Structures of Nitridorhenium(V) and Nitridotechnetium(V) Complexes with N,N-[(Dialkylamino)(thiocarbonyl)-N '-(2-hydroxyphenyl)benzamidines

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DOI: 10.1002/zaac.201100134 Syntheses and Structures of NitridorheniumV and NitridotechnetiumV Complexes with N,N-[Dialkylaminothiocarbonyl]-N'-2-hydroxyphenylbenzamidines Hung Huy Nguy

Trang 1

DOI: 10.1002/zaac.201100134

Syntheses and Structures of Nitridorhenium(V) and Nitridotechnetium(V)

Complexes with

N,N-[(Dialkylamino)(thiocarbonyl)]-N'-(2-hydroxyphenyl)benzamidines Hung Huy Nguyen,*[a] Thi Nguyet Trieu,[a] and Ulrich Abram*[b]

Keywords: Rhenium; Technetium; Tridentate benzamidine; Nitrido complexes; Structure analysis

Abstract [MNCl2(PPh3)2] complexes (M = Re, Tc) react with

N-[(di-alkylamino)(thiocarbonyl)]-N'-(2-hydroxyphenyl)benzamidines (H2L1)

with formation of neutral, five-coordinate nitrido complexes of the

Introduction

Despite the fact that bidentate

N-[(dialkylamino)(thiocar-bonyl)]benzamidines (I) are well known chelators and a large

number of their complexes with many transition metal ions,

such as Ni2+, Pd2+, Pt2+, Co3+, Cu2+, Ag+, and Au+have been

extensively studied during the last three decades,[1]

surpris-ingly less is known about tridentate benzamidines Recently,

we have reported about such ligands (II), which can be

pre-pared by reactions of benzimidoyl chlorides with

functional-ized primary amines.[2]These ligand systems allow a variety

of modifications in the periphery of their chelating system,

which tune their properties and also give access to amino acid

derivatives and bioconjugation.[3]Some complexes of the new

ligands, preferably derivatives of thiosemicarbazides, reveal

promising cytotoxic properties against human MCF-7 breast

cancer cells.[4]

Particularly the coordination chemistry of rhenium and

tech-netium with

N-[(dialkylamino)(thiocarbonyl)]-N'-(2-hydroxy-* Dr N H Huy

E-Mail: nguyenhunghuy@hus.edu.vn

* Prof Dr U Abram

Fax: +49-30-838-2676

E-Mail: ulrich.abram@fu-berlin.de

[a] Inorganic Chemistry Department

Hanoi University of Science

19 Le Thanh Tong

Hanoi, Vietnam

[b] Institute of Chemistry and Biochemistry

Freie Universität Berlin

Fabeckstrasse 34–36

14195 Berlin, Germany

composition [MN(L1)(PPh3)] The products have distorted square-pyramidal coordination spheres with each a tridentate, double-deproto-nated benzamidine and a PPh3ligand in their basal planes

phenyl)benzamidines (H2L1) has been intensively studied Most of the isolated complexes with these ligands have rhenium(V) or oxotechnetium(V) cores Five-coordinate oxo-rhenium(V) and oxotechnetium (V) complexes,[2] as well as

cis methoxo compounds,[5]‘3+2’ mixed-ligand complexes,[6] and dimeric oxorhenium(V) complexes[5] were isolated and structurally characterized Only one exceptional compound is

an octahedral technetium(III) complex.[2] In continuation of our systematic studies on thiocarbamoylbenzamidinato com-plexes of rhenium and technetium, here we report the synthesis and molecular structures of nitridorhenium(V) and nitridotech-netium(V) complexes with ligands of the type H2L1.

Results and Discussion

[MNCl2(PPh3)2] compounds (M = Re, Tc) are common

start-ing materials for the synthesis of ReV and TcV nitrido com-plexes The compounds are sparingly soluble in organic sol-vents However, they slowly dissolve in stirred solutions of

H2L1 in CH2Cl2 at room temperature with formation of deep red solutions, from which red crystalline products of the

com-position [MN(L1)(PPh3)] (M = Re, Tc) can be isolated in high

yields The addition of a supporting base like Et3N accelerates the consumption of [ReNCl2(PPh3)2], whereas [TcNCl2(PPh3)2] is more labile than its analogous rhenium compound and readily reacts with the ligand without the addi-tion of a base (Scheme 1) The complexes are readily soluble

in polar organic solvents such as CHCl3 or THF They are stable as solids as well in solution Their structures were stud-ied by common spectroscopic methods.

Infrared spectra of the complexes exhibit strong bathochro-mic shifts of the νC=Nstretches from the range between 1610 and 1620 cm–1 of the non-coordinated benzamidines to the

1510 cm–1region This indicates chelate formation with a large degree of π-electron delocalization within the chelate rings and has been observed before for the corresponding oxo complexes.[2]The absence of absorptions in the regions around

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Nitridorhenium(V) and Nitridotechnetium(V) Complexes

Scheme 1 Reactions of [MNCl2(PPh3)2] (M = Re, Tc) with H2L1 The

addition of NEt3is only required for the rhenium compound (see text)

3350 cm–1and 3150 cm–1, in which the νNHand νOHstretches

are detected in the spectra of the uncoordinated H2L1, indicates

the expected double deprotonation of the ligands during

com-plex formation Absorption bands of medium intensity around

1065 cm–1are assigned to Re≡N and Tc≡N stretches.[7]

The NMR spectra of the complexes provide additional

evi-dence for the proposed composition and the molecular

struc-tures of the complexes A hindered rotation around the C–NR2

bonds results in magnetic inequivalence of the two residues R.

Thus, two triplet signals of the methyl groups in the –NEt2

residues are observed in the 1H NMR spectra of

[ReN(L1a)(PPh3)] and [TcN(L1a)(PPh3)] at room temperature.

However, the proton signals of the two methylene groups,

which should consequently be two quartet signals, appear as

four multiplet resonances including two overlapping signals

at 3.80 ppm and two well separated signals at 3.68 ppm and

4.17 ppm in the spectrum of the rhenium compound This

pat-tern of the methylene signals can be explained by the rigid

structure of the tertiary amine group, which makes the

methyl-ene protons magnetically inequivalent with respect to their

ax-ial and equatorax-ial positions The rigidity of morpholinyl moiety

of {L1b}2– in [ReN(L1b)(PPh3)] results in four broad singlet

signals at 3.59, 3.81, 3.96, and 4.32 ppm, which correspond to

two CH2–N protons and two CH2–O protons The signals of

four other protons appear as two overlapping broad singlet

sig-nals at 3.70 and 4.16 ppm Similar coupling patterns are

ob-served in the spectra of the corresponding technetium

com-plexes.

The13C NMR spectra of the complexes are easier to explain,

since their patterns are only influenced by hindered rotation

around the C–NR2bonds Consequently, two separated signals

for each CH2 and CH3 carbon atoms in the NEt2groups and/

or CH2–N and CH2–O atoms in the morpholinyl units appear.

The presence of triphenylphosphine ligands in the coordination

sphere of the products is confirmed by each one singlet signal

in their31P NMR spectra, appearing around 30.0 ppm for the

rhenium compounds and around 45 ppm for the technetium

complexes.

ESI+ mass spectra of the rhenium complexes show intense

signals corresponding to the expected [M + Na]+and [M + H]+

ions.

X-ray structure analyses were performed for [ReN(L1b)(PPh3)]

and [TcN(L1b)(PPh3)] Suitable single crystals were obtained by

slow evaporation of CH2Cl2/MeOH solutions of the compounds.

Figure 1 illustrates the molecular structure of the rhenium

com-plex Selected bond lengths and angles are presented in Table 1.

The structure of [TcN(L1b)(PPh3)] is virtually identical Thus,

no extra Figure is presented for the technetium compound The corresponding bond lengths and angles, however, are also con-tained in Table 1.

Figure 1 Ellipsoid representation of the molecular structure of

[ReN(L1b)(PPh3)] Hydrogen atoms are omitted for clarity Thermal ellipsoids represent 50 per cent probability

Table 1 Selected bond lengths /Å and angles /° in [MN(L1b)(PPh3)]

(M = Re, Tc) complexes The atomic labeling scheme of Figure 1 is

also applied for the technetium complex

[ReN(L1b)(PPh3)] [TcN(L1b)(PPh3)]

The metal atoms show distorted square-pyramidal coordina-tion spheres with the nitrido ligands in apical posicoordina-tions Such

an assignment of the molecular geometry is supported by the

corresponding τ values of 0.25 (technetium compound) and

0.24 (rhenium complex) They clearly indicate that the com-plexes under study are better described with a

square-pyrami-dal coordination sphere (τ = 0) than as trigonal bipyramids (τ =

1) The thiocarbamoylbenzamidines coordinate meridional to the metal atoms as tridentate dianionic ligands as in their previ-ously reported oxo complexes The remaining positions in the basal planes of the square pyramids are occupied each by a triphenylphosphine ligand The metal atoms lie above the equatorial planes towards the nitrido ligands by 0.528(2) Å for

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H H Nguyen, T N Trieu, U Abram

ARTICLE

the rhenium complex and 0.544(2) Å for the technetium

com-pound The N10–Re–X angles (X = equatorial donor atom) fall

in the range between 92.6 and 111.5°, the corresponding N10–

Tc–X angles are between 93.3 and 112.0° These values are in

complete agreement with the typical bonding situation of

square-pyramidal ReVN and TcVN complexes.[8]

The Re–N10 and Tc–N10 distances of 1.645(6) and

1.619(5) Å are within the expected range of rhenium or

techne-tium–nitrogen triple bonds.[9]The six-membered chelate rings

are strongly distorted for both complexes, with main deviations

of 0.413(4) Å (rhenium complex) and 0.419(4) Å (technetium

complex) from the mean least-square planes each for the

nitro-gen atoms N5 Nevertheless, a considerable delocalization of

π-electron density is observed This is indicated by the C–S and

C–N bond lengths inside the chelate rings, which all fall within

the range between carbon–sulfur and carbon–nitrogen single

and double bonds This bond length equalization is even

extended to the C2–N6 bonds, which are significantly shorter

than expected for single bonds All the MO, MN and M–S

bonds are slightly longer than those in the corresponding oxo

complexes.[2,5]This can be understood by the larger steric bulk

of the M≡N triple bond, which results in larger

N≡MX(equatorial) angles and consequently in a somewhat less

effective overlap between the ligand and the d orbitals of the

metal ions Details have been discussed previously on a series

of oxo, nitrido and phenylimido compounds of technetium and

rhenium with 1,2-dicyanoethene-1,2-dithiolate.[10]

Conclusions

The ready synthesis and the stability of the nitrido complexes

under study and the promising biological properties of the

re-lated oxo compounds[2–4] recommend transition metal

com-plexes with ligands of the thiocarbamoylbenzamidine family

for further consideration with regard to medical and nuclear

medical applications Ongoing studies with tri- to pentadentate

ligands with peripheral coupling positions for biomolecules are

presently underway in our laboratories They also include

ni-trido complexes with the ligands discussed in this paper as

well as tetradentate systems derived from diamines.

Experimental Section

Materials and Measurements

All reagents used in this study were reagent grade and used without

further purification Solvents were dried and used freshly distilled

un-less otherwise stated [ReNCl2(PPh3)2][11]and [TcNCl2(PPh3)2][12]were

prepared by standard procedures The synthesis of H2L1ligands were

described in a previous paper.[2]

Infrared spectra were recorded from KBr pellets with a Shimadzu FT

instrument in the range 400–4000 cm–1 Positive electrospray

ioniza-tion mass spectra (ESI+MS) were measured with an Agilent 6210

ESI-TOF (Agilent Technologies) (results are given in the form: m/z, %

based peak, assignment) Elemental analyses were determined using

a Heraeus vario EL elemental analyzer The technetium content was

determined by liquid scintillation measurements NMR spectra were

taken at 25 °C with a JEOL 400 MHz multinuclear spectrometer

Radiation Precautions

99

Tc is a weak β-emitter All manipulations with this isotope were performed in a laboratory approved for the handling of radioactive materials Normal glassware provides adequate protection against the low-energy β-emission of the technetium compounds Secondary X-rays (bremsstrahlung) play an important role only when larger amounts

of99Tc are used

Synthesis of [ReN(L1)(PPh3)]

Solid [ReNCl2(PPh3)2] (80 mg, 0.1 mmol) was added to a stirred solu-tion of H2L1(0.1 mmol) in CH2Cl2 (5 mL) The mixture was stirred

at room temperature for 15 min and then 3 drops of Et3N were added This resulted in a complete dissolution of [ReNCl2(PPh3)2] and the formation of a red solution The solvent was removed under vacuum, and the residue was crystallized by slow evaporation of a CH2Cl2/ MeOH solution as red blocks The side products (HNEt3)Cl and PPh3

remain in the residual MeOH Alternatively, the crude reaction product can be washed twice with methanol before re-dissolution in CH2Cl2

for crystallization

Data for [ReN(L 1a )(PPh 3)] (R1= R2 = Et): Yield: 60 mg, 76 %

Ele-mental analysis Calcd for C36H34N4OPSRe: C, 54.88; H, 4.35; N,

7.11; S, 4.07 % Found: C, 54.78; H, 4.30; N, 7.07; S, 4.15 % IR:

ν = 3059 (w), 2978 (w), 2932 (w), 1512 (vs), 1492 (vs), 1477 (vs),

1439 (s), 1393 (m), 1354 (m), 1254 (vs), 1096 (m), 1065 (m), 1026 (w), 744 (m), 686 (s), 528 (s), 505 (m).1 H NMR (CDCl3): δ = 1.08 (t, J = 7.0 Hz, 3 H, CH3), 1.26 (t, J = 7.1 Hz, 3 H, CH3), 3.68 (m, 1

H, CH2), 3.80 (m, 2 H, CH2), 4.17 (m, 1 H, CH2), 6.27 (t, J = 7.6 Hz,

1 H, PhOH), 6.47 (d, J = 7.8 Hz, 1 H, PhOH), 6.62 (t, J = 7.0 Hz, 1

H, PhOH), 6.83 (d, J = 7.8 Hz, 1 H, PhOH), 7.26 (t, J = 7.3 Hz, 2 H,

Ph), 7.35 (m, 10 H, Ph + PPh3), 7.79 (m, 8 H, Ph + PPh3).13 C NMR

(CDCl3): δ = 12.8, 13.2 (CH3), 46.3, 47.7 (CH2), 117.0–135.8 (Caromatic), 141.3 (Caromatic–N), 162.1 (Caromatic–O), 166.8 (C=N), 171.6 (C = S).31 P NMR (CDCl3): δ = 29.6 (s) ESI+MS (m/z): 811, 100 %,

[M + Na]+; 789, 40 %, [M + H]+

Data for [ReN(L 1b )(PPh 3)] (NR1R2 = morph): Yield: 64 mg, 80 %.

Elemental analysis Calcd for C36H32N4O2PSRe: C, 53.92; H, 4.02; N,

6.99; S, 4.00 % Found: C, 53.87; H, 4.15; N, 7.10; S, 4.09 % IR: ν =

3051 (w), 2970 (w), 2905 (w), 2858 (w), 1507 (vs), 1477 (vs), 1435 (s), 1388 (s), 1357 (w), 1257 (s), 1219 (m), 1096 (m), 1068 (m), 1026 (m), 745 (m), 690 (s), 528 (s), 505 (m).1 H NMR (CDCl3): δ = 3.59

(br s, 1 H, NCH2), 3.70 (br s, 2 H, NCH2), 3.81 (br s, 1 H, NCH2), 3.96 (br s, 1 H, OCH2), 4.16 (br s, 2 H, OCH2), 4.32 (br s, 1 H, OCH2), 6.27 (t, J = 7.6 Hz, 1 H, PhOH), 6.49 (d, J = 7.8 Hz, 1 H, PhOH), 6.64 (t, J = 7.6 Hz, 1 H, PhOH), 6.84 (d, J = 7.9 Hz, 1 H, PhOH), 7.27 (t, J = 7.3 Hz, 2 H, Ph), 7.37 (m, 10 H, Ph + PPh3), 7.77 (m, 8 H, Ph +PPh3).13 C NMR (CDCl3): δ = 48.8, 49.7 (NCH2), 66.7, 66.9 (OCH2), 117.2–135.7 (Caromatic), 140.9 (Caromatic–N), 163.0 (Caromatic–O), 167.1 (C=N), 171.3 (C = S).31 P NMR (CDCl3): δ = 28.8

(s) ESI +MS (m/z): 825, 100 %, [M + Na]+; 803, 40 %, [M + H]+

Synthesis of [TcN(L1)(PPh3)].

The technetium complexes were prepared following the procedure de-scribed for their analogous rhenium complexes except that the precur-sor [TcNCl2(PPh3)2] was used and no NEt3was added

Data for [TcN(L 1a )(PPh 3)] (R1= R2 = Et): Yield: 59 mg, 84 %

Ele-mental analysis Calcd for C36H34N4OPSTc: Tc, 14.1 % Found: Tc,

14.1 % IR: ν = 3051 (w), 2970 (w), 2924 (w), 1504 (vs), 1477 (vs),

Trang 4

Nitridorhenium(V) and Nitridotechnetium(V) Complexes

1434 (s), 1396 (m), 1350 (m), 1307 (m), 1258 (vs), 1095 (m), 1057

(m), 1026 (w), 798 (m), 741 (m), 690 (s), 528 (s), 497 (m) cm–1.1 H

NMR (CDCl3): δ = 1.09 (t, J = 7.1 Hz, 3 H, CH3), 1.26 (t, J = 7.1 Hz,

3 H, CH3), 3.67 (m, 1 H, CH2), 3.75 (m, 2 H, CH2), 4.00 (m, 1 H,

CH2), 6.23 (t, J = 7.5 Hz, 1 H, PhOH), 6.37 (d, J = 7.7 Hz, 1 H,

PhOH), 6.63 (t, J = 7.1 Hz, 1 H, PhOH), 6.79 (d, J = 7.8 Hz, 1 H,

PhOH), 7.26 (t, J = 7.4 Hz, 2 H, Ph), 7.30 (m, 10 H, Ph + PPh3), 7.73

(m, 8 H, Ph + PPh3).31 P NMR (CDCl3): δ = 45.4 (s).

Data for [TcN(L 1b )(PPh 3)] (NR1R2 = morph): Yield: 63 mg, 89 %.

Elemental analysis Calcd for C36H32N4O2PSTc: Tc, 13.8 % Found:

Tc, 13.9 % IR: ν = 3051 (w), 2970 (w), 2909 (w), 2843 (w), 1498

(vs), 1477 (vs), 1431 (s), 1400 (s), 1357 (w), 1312 (m), 1265 (s), 1215

(m), 1095 (m), 1060 (m), 1026 (m), 844 (m), 748 (s), 691 (s), 524 (s),

505 (m) cm–1 1 H NMR (400 MHz, CDCl3): δ = 3.61 (br s, 1 H,

NCH2), 3.70 (m, 2 H, NCH2), 3.76 (br s, 1 H, NCH2), 3.98 (br s, 1

H, OCH2), 4.05 (br m, 2 H, OCH2), 4.20 (br s, 1 H, OCH2), 6.22 (t,

J = 7.5 Hz, 1 H, PhOH), 6.38 (d, J = 7.8 Hz, 1 H, PhOH), 6.65 (t, J =

7.6 Hz, 1 H, PhOH), 6.79 (d, J = 7.8 Hz, 1 H, PhOH), 7.22 (t, J =

7.4 Hz, 2 H, Ph), 7.34 (m, 10 H, Ph + PPh3), 7.71 (m, 8 H, Ph +

PPh3).31P NMR (CDCl): δ = 44.6 (s).

X-ray Crystallography

The X-ray diffraction data were collected with a STOE IPDS

diffrac-tometer with Mo-Kαradiation The structures were solved by the

Pat-terson method using SHELXS-97.[13] Subsequent Fourier-difference

map analyses yielded the positions of the non-hydrogen atoms

Refine-ment was performed using SHELXL-97.[13]The positions of hydrogen

atoms were calculated for idealized positions and treated with the

‘rid-Table 2 Crystal data and refinement results.

[ReN(L1b)(PPh3)] [TcN(L1b)(PPh3)]

Formula C36H32N4O2PReS C36H32N4O2PSTc

Dcalcd./g·cm–3 1.671 1.485

No of independent /Rint 8499 / 0.1023 8518 / 0.1245

ing model’ option of SHELXL-97 Crystal data and more details of the data collections and refinements are contained in Table 2 Additional information on the structure determinations have been deposited at the Cambridge Crystallographic Data Centre

Acknowledgement

We thank the Deutscher Akademischer Austauschdienst (DAAD) for generous support H H Nguyen is additionally grateful to Vietnam’s National Foundation for Science and Technology Development for the

financial support through project 104.02–2010.31

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Received: March 21, 2011 Published Online: June 6, 2011

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