PREPARATION OF NANOHYDROGEL STRUCTURE BASED ON PLA-PCL-PEG-PCL-PLA PENTABLOCK COPOLYMER FOR CURCUMIN DELIVERY Nguyễn Văn Cường1,*, Trần Thị Quỳnh Mai1, Nguyễn Văn Bời1, Huỳnh Đại Phú2 1D
Trang 1PREPARATION OF NANOHYDROGEL STRUCTURE BASED ON PLA-PCL-PEG-PCL-PLA PENTABLOCK COPOLYMER FOR CURCUMIN DELIVERY
Nguyễn Văn Cường1,*, Trần Thị Quỳnh Mai1, Nguyễn Văn Bời1, Huỳnh Đại Phú2
1Department of Chemical Engineering, Industrial University of HCMC 2Department of Material Engineering, Ho Chi Minh University of Technology
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1 Junmin Zhu and Roger E Marchant Design Properties Of Hydrogel Tissue-Engineering Scaffolds Expert Rev Med Devices 8(5): 607-626 (2011).
2 T Kissel, Y Li, F Unger, ABA-triblock copolymers from biodegradable polyester A-blocks and hydrophilic poly(ethylene oxide) B-blocks as a candidate for in situ forming hydrogel delivery systems for proteins, Adv Drug Deliv Rev.,
54, 99-134 (2002).
3 D P Huynh, M K Nguyen, B S Pi, M S Kim, S Y Chae, K C Lee, B S Kim, S W Kim, D S Lee, Functionalized injectable hydrogels for controlled insulin delivery, Biomaterials, 29, 2527-2534 (2008).
4 H.Đ Phú, Hydrogel y sinh nhạy nhiệt độ trên cơ sở triblockcopolyme PCLA-PEG-PCLA.Tạp chí Hóa Học, 2: 463-468 (2013).
References
In this article, thermo-sensitive
hydrogel based on poly(lactide acid)-
poly( -caprolactone)-poly(ethylene ɛ
glycol)-poly( -caprolactone)-poly(lactide ɛ
acid) (PLA-PCL-PEG-PCL-PLA) was
successfully synthesized by using
inverting test-tube method
The composition and surface
morphology of hydrogel were
characterized by FT-IR and SEM The
SEM image indicated the hydrogel was
composed of copolymeric network and
nano-sized particles The curcumin was
entrapped into copolymeric hydrogel In
vitro drug release behavior was studied in
PBS buffer
The result showed that controlled
curcumin release was observed The
release capacity decreased with
increasing curcumin entrapped into
hydrogel, and it decreased with increasing
of copolymer content
Abstract
a Synthesis hydrogel pentablock copolymer
PLA-PCL-PEG-PCL-PLA hydrogel
based on central PCL and PLA blocks and end PEG blocks showed temperature-dependent sol-gel transition (Figure 1)
The identities of hydrogel and curcumin-loaded hydrogel were evaluated
by FTIR (Figure 2)
Interior morphology of hydrogel was investigated by SEM Hydrogel was frozen in liquid nitrogen and lyophilized for
12 h before the test (Figure 3)
b In vitro drug release behavior
In vitro release behavior of curcumin from PLA-PCL-PEG-PCL-PLA hydrogel was investigated, which is presented in Figure 4
Introduction
Methods and Materials
In this contribution, we synthesized hydrogel based on PLA-PCL-PEG-PCL-PLA copolymer by using test-tube inverting method The structures of hydrogel and curcumin-loaded hydrogel were confirmed
by FT-IR The porous and 3D structures were observed by SEM Additionally, some nanosized micelles were also seen in SEM The in vitro drug release behavior show that, hydrogel releases drug by swelling, diffusing and degradation than dissolving The hydrogel exhibited the controlled drug release Importantly, this study has demonstrated that the hydrogel can provide delivery of curcumin for biomedical applications
Future work
Medicines derived from plants play an
important role in the healthcare of many
cultures, both ancient and modern
Curcumin (diferuloylmethane, a
polyphenol) is an active principle of the
perennial herb Curcuma longa (commonly
known as turmeric) Curcumin, a naturally
occurring polyphenolicphytoconstituent
which presents anticancer, antioxidant,
anti-inflammatory, hyperlipidemic,
antibacterial, wound healing and
hepatoprotective activities The
therapeutic efficacy of curcumin is limited
due to its poor aqueous solubility and
extensive first pass metabolism
Hydrogels are water-swollen polymeric
networks and crosslinked polymeric
structures produced by the simple reaction
of one or more monomers, or by
association bonds such as hydrogen
bonds and strong Van der Waals
interactions between chains
Hydrogels are extremely useful as
biomaterials and can be synthesized to be
responsive for external stimuli For the last
decades, scientists concentrate to
prepared hydrogel based on diblock and
triblock copolymers such as: PEG-PCL,
PCL-PEG-PCL, PLA-PEG-PLA and so on
They realized that high molecular weight
PCL need about one year to complete in
vitro biodegradation Therefore, the
combination of fast degradation PLA with
PCL was to improve the biodegradation
and controlled drug release
Primary Results and
Discussion
Figure 2 FTIR of pentablock popolymer (a),
catechin (b) and catechin-loaded hydrogel (c).
Materials:
Pentablock copolymer PLA-PCL-PEG-PCL-PLA was synthesized by ring opening copolymerization of ε-caprolactone and lactide acid with macroinitiator as poly(ethylene glycol)
Curcumin was purchased from Vietnam Institute Dietary Supplement (Hanoi, Vietnam) All chemicals used in this study were of reagent grade
Methods:
The hydrogel was synthesized by using
an inverting test-tube method through a
10 ml tightly screw-capped vial with inner diameter of 12 mm The copolymer solutions were prepared by dissolution various amount of copolymer in distilled water The solutions were transferred into test tube and slowly heated to 80°C, then the samples was slowly cool down
The sol-gel transition was observed visually by inverting the vials and condition of gel was defined in 1 min [4]
For curcumin-loaded hydrogel, the 20%
(w/w) copolymeric solution contained curcumin was prepared and formed hydrogel as described above.
Figure 3 SEM of hydrogel with different
magnifications
Figure 4 In vitro drug release behavior from
hydrogel
0 20 40 60 80
100
120 20% - 1mg 20% - 1.5mg 30% - 1mg
Time (min)
Dr Nguyen Van Cuong
Department of Chemical Engineering
Email: nvc@hui.edu.vn
Figure 1 Copolymeric solution at low
temperature (a) and gel form at 37 oC (b)