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Cosmetics rarely are the cause of contact urticaria; however, many cases of “subjective irritation” might actually represent very mild nonimmunological contact reactions, caused especial

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Fatal Attractiveness: The Shady Side

ANTON C DE GROOT, MD, PhD

C osmetics (which include skin-care products and

decorative cosmetics) and toiletries (which in-

clude soap, shampoo, bath-foam, and tooth-

paste) are used by nearly everyone Not surprisingly

and inevitably, these products will cause side effects in

some consumers Contrary to what the title of this

article suggests, serious adverse reactions from cosmet-

ics are infrequent; however, mild unwanted effects are

experienced by over 10% of the population.1,2 Diagnos-

ing and treating patients with such reactions are part of

the daily routine of dermatologists In this contribution

cosmetic-related irritation, photosensitivity, and imme-

diate contact reactions will be discussed briefly; contact

allergy as a side effect of cosmetics is presented in more

detail, highlighting recently emerged important cos-

metic allergens.‘-”

Irritation

There are two forms of irritation: subjective and objec-

tive Subjective irritation may be defined as chemically

induced burning, stinging, itching, or other skin dis-

comfort without visible, objective signs of inflamma-

tion It is estimated that between 1 and 10% of all

(women) cosmetic users note this discomfort, primarily

on the face Objective irritation is defined as nonimmu-

nologically mediated inflammation of the skin Its signs

are usually mild erythema and scaling, but frank der-

matitis may occur Irritation may be observed with

cosmetic products containing detergents such as soap,

shampoo, and bath/shower foam Atopics and elderly

people with good/excessive hygiene are particularly

susceptible to developing this side effect, mainly during

the winter when humidity is low Itching usually starts

on the legs, arms and hips The humid climate in, and

anatomical occlusion of, the axillae favor irritant re-

sponses to deodorants and antiperspirants Surfactants

and emulsifiers present in moisturizing or emollient

creams may also cause irritation, especially when ap-

plied to facial skin Daily application of eye makeup

cosmetics and removal with cleansing products often

irritate the sensitive skin of the eyelids

-~ -~~-

From the Department of DematoIogy, Carolus-Liduina Hospital, ‘s-

Hertogerlbosch, The Netherlands

Address correspondence to Anton C De Groot, M.D., Carolus-Liduina

Hospital, P.0 Box 3 102, 5200 BD’s-Hertogenbosch, The Netherlands

0 1998 by Elseuier Science Inc

Photosensitivity

With the exception of the epidemic caused by the halo- genated salicylanilides in the 196Os, photosensitivity has accounted for only a small proportion of cosmetic- related side effects In a study from the United States, photoallergy and phototoxicity were responsible for only 9 reactions in 713 patients investigated for cos- metic dermatitis.7 Musk ambrette, a fragrance fixative formerly used in many aftershaves, until recently was a major cause of photocontact allergy, often leading to persistent light reactions;8 an association with chronic actinic dermatitis has also been suggested.9 In 1985, the International Fragrance Association (IFRA) recom- mended that musk ambrette not be utilized in products

in contact with skin; since then, the numbers of relevant photocontact allergic reactions have decreased consid- erably Nowadays, paradoxically as this may seem, ul- traviolet (UV) filters have become important causes of photocontact allergy The increased public awareness of the risks of premature skin aging and cancer caused by exposure to sunlight has led to more extensive use of

UV filters, not only in sunscreen preparations but also

in skin-care products (notably facial creams); this is the major cause of the recent increase of photocontact aller- gic reactions to UV filters Patients with photosensitive diseases, such as chronic polymorphic light eruption (CPLE) and chronic actinic dermatitis, who use sun- screens habitually are particularly sensitive to develop- ing photocontact allergy Most cases are caused by the benzophenones (notably oxybenzone) and the UVA- filtering dibenzoylmethanes (notably isopropyl diben- zoylmethane, butyl methoxydibenzoylmethane).lOJi Other less frequent photosensitizers are p-aminoben- zoic acid @‘ABA), octyl dimethyl PABA, and ethyl- hexyl-p-methoxycinnamate Possibly, the frequency of photocontact allergy to cosmetics is underestimated When such reactions occur to sunscreens, the resulting photoallergic reaction may be interpreted by the patient

as the failure of the product to adequately protect against the sun’s rays rather than as an adverse reaction

to the product; medical consultation is then not sought

In addition, probably only a minority of dermatologists

in private practice perform photopatch testing, result- ing in missed cases of photocontact allergy

0738-081X/98/$19.00

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Clinics izz Dermatology l 1998;16:167-179

Immediate Contact Reactions

(Contact Urticaria)

The contact urticaria syndrome may comprise cutane-

ous reactions (localized urticaria, generalized urticaria)

as well as extracutaneous reactions (bronchial asthma,

rhinoconjunctivitis, otolaryngeal symptoms, gastroin-

testinal symptoms, anaphylactic shock) Immediate

contact reactions are either nonimmunological (caused

by nonimmunological release of histamine and other

vasoactive substances) or immunological (IgE-mediat-

ed) Cosmetics rarely are the cause of contact urticaria;

however, many cases of “subjective irritation” might

actually represent very mild nonimmunological contact

reactions, caused especially by ingredients in cosmetic

products known to induce such symptoms, such as

sorbic acid, benzoates, and cinnamic aldehyde In ad-

dition, immediate contact reactions to fragrance mate-

rials in perfumes may induce or worsen respiratory

problems such as shortness of breath, asthma, and

sneezing People with respiratory allergy commonly

experience aggravation of their complaints around cos-

metic counters, candle shops, and from perfumes worn

by other people, for example, in church.‘j

Contact Allergy

Epidemiology

Allergic contact reactions to cosmetics often go unrec-

ognized for several reasons.7 (1) Frequently patients

have used the causative cosmetics for many years; the

development of skin problems from such products con-

flicts with the consumer’s perception of allergy, which

is based on the assumption that a new cosmetic has to

be introduced (2) Cosmetic allergy is sometimes man-

ifested by mild reactions only, for example, itching,

faint erythema, and mild scaling of the eyelids (3)

Cosmetic dermatitis may sometimes be noticed, but

wrongly interpreted Psoriasis of the face may be exac-

erbated by cosmetic dermatitis; dermatitis caused by

emollient creams interpreted as worsening of dry skin

or atopic dermatitis for which it was applied; and con-

tact allergy to sunscreens as failure of the product to

adequately protect the skin against the sun’s rays

Nevertheless, allergic contact dermatitis to cosmetic

products is frequently observed Of dermatological pa-

tients patch tested for suspected allergic contact derma-

titis, some 10% are allergic to cosmetic products.12 Six to

11% of routinely tested individuals react to the fra-

grance mix, a mixture of 8 commonly used fragrance

materials to detect sensitization to perfumes In the list

of frequent allergens, the fragrance mix usually is num-

ber 2 after nickel sulfate.6 An estimated 1% of the gen-

eral population is allergic to fragrances, and 2-3% are

allergic to substances that may be present in cosmetics

and toiletries 12,13

Clinical Picture

Sometimes, contact allergic dermatitis from cosmetic products can be easily recognized Examples include reactions to deodorant, eye shadow, perfume dabbed behind the ears or on the wrist, and lipstick In more than half of all cases, the diagnosis of cosmetic allergy is suspected neither by the patient nor by the doctor.7 The clinical picture of allergic cosmetic dermatitis depends on the type of products used (and conse- quently, the sites of application) and the degree of the patient’s sensitivity Usually, cosmetics and their ingre- dients are weak allergens, and the dermatitis resulting from cosmetic allergy is mild: erythema, mild edema, desquamation, and papules Weeping vesicular derma- titis rarely occurs, although some products, especially the permanent hair dyes, may cause fierce reactions, notably on the face and ears and less on the scalp Allergic reactions on the scalp tend to be seborrheic dermatitis-like with temporary hair loss, (HB van der Walle, personal communication, 1997; personal unpub- lished observations)

Contact allergy to fragrances may resemble nummu- lar eczema, seborrheic dermatitis, sycosis barbae, or lupus erythematosus 6 Lesions in the skin folds may be mistaken for atopic dermatitis Dermatitis due to per- fumes or toilet water tends to be “streaky” Allergy to toluenesulfonamide/formaldehyde resin in nail polish may affect the fingers, 14 but most allergic reactions are located on the eyelids, in and behind the ears, in the neck, and sometimes around the anus or perivulval The typical patient suffering from allergic cosmetic dermatitis is a woman aged 20-45 years with mild dermatitis of the eyelids The face itself is also fre- quently involved, and often the dermatitis is limited to the face and/or eyelids Other predilection sites for cosmetic dermatitis are the neck, the arms and the hands; however, all parts of the body may be involved Most often, the cosmetics have been applied to previ- ously healthy skin (especially the face), nails or hair Allergic cosmetic dermatitis may also be caused by products used on previously damaged skin, for exam- ple to treat or prevent dry skin of the arms and legs or irritant or atopic hand dermatitis.6

The Products Causing Cosmetic Allergy

Most allergic reactions are caused by cosmetics that remain on the skin: “stay-on” or “leave-on” products: skin care products (moisturizing and cleansing creams, lotions, milks, tonics), hair cosmetics (notably hair dyes), nail cosmetics (nail polish, nail hardener), de- odorants and other perfumes, and facial and eye make-up products.3,6,7,15 “Rinse-off” or “wash-off” products such as soap, shampoo, bath foam, and shower foam rarely elicit or induce contact allergic reactions This may be explained by the dilution of the

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Clinics in Dermatology l 1998;16:167-179 THE SHADY SIDE OF COSMETICS 169

product (and, consequently, of the [potential] allergen)

under normal circumstances of use, and the fact that the

product is removed from the skin by washing after a

short period of time One exception to this general rule

is allergy to the surfactant cocamidopropyl betaine,

which has caused many reactions to shampoo in con-

sumers and occupational dermatitis in hairdressers,

and to shower gels (see later).16J7J8

Of course, trends in cosmetic usage, e.g the growing

cosmetic market for men and the development of new

products (“kiddy cosmetics”, hair gel), may influence

the situation

The Allergens

Although there are many publications on contact al-

lergy to cosmetics and toiletries,>5 only 2 studies have

systematically investigated the allergens in such prod-

ucts.7J5 In both studies, fragrances and preservatives

were the most common causative ingredients in allergic

cosmetic dermatitis Other important allergens are the hair

color y-phenylenediamine (and related permanent dyes),

lanolin and its derivatives, the nail lacquer resin toluene-

sulfonamide/formaldehyde resin, UV-filters (more often

photocontact allergy), and various emulsifiers More re-

cently, the surfactant cocamidopropyl betaine and the

preservative methyldibromo glutaronitrile have emerged

as important cosmetic allergens.16-20

Preservatives are added to water-containing cosmetics

to inhibit the growth of nonpathogenic and pathogenic microorganisms, which may cause degradation of the product or endanger the health of the consumer.21-23 Formaldehyde and Formaldehyde Donors

FORMALDEHYDE Formaldehyde is a frequent sensitizer and ubiquitous allergen Routine testing in patients with suspected allergic contact dermatitis yields prev- alence rates of sensitization of 3% or more, but most cases are from non-cosmetic sources The cosmetic in- dustry uses free formaldehyde almost exclusively in rinse-off products, which rarely gives rise to cosmetic allergy The literature on formaldehyde allergy has been reviewed.21,22

FORMALDEHYDE DONORS Formaldehyde donors are pre- servatives that, in the presence of water, release form- aldehyde Therefore, cosmetics preserved with such chemicals will contain free formaldehyde, the amount depending on the preservative used, its concentration, and the amount of water present in the product Form- aldehyde donors used in cosmetics and toiletries in- clude quaternium-15, imidazolidinyl urea, diazolidinyl urea, 2-bromo-2-nitropropane-1,3-diol, and DMDM hy- dantoin Quaternium-15 releases the most, imidazolidi- nyl urea the least free formaldehyde.2” Contact allergy

Fragrances

Fragrances are the most frequent cause of cosmetic

allergy, both from products primarily used for their

scent (perfumes, colognes, eaux de toilette, aftershave,

deodorants) and from other scented products.6 Approx-

imately 3000 chemical substances (of which 300-400 are

of natural origin) are used in the fragrance industry A

perfume is a creative composition of fragrance materi-

als, of which it may contain from a few to over 300

Perfumes contain approximately 12-20% of the per-

fume compound, toilet water 5-8%, colognes 2-5%, and

cosmetics 0.5% Most fragrance-sensitive patients are

identified by positive patch tests to personal products,

the “fragrance-mix”, or the North American Contact

Dermatitis Group (NACDG) perfume-screening series

The “fragrance mix” contains 8 commonly used fra-

grance ingredients: cw-amylcinnamic aldehyde, cin-

namic alcohol, cinnamic aldehyde, eugenol, geraniol,

hydroxycitronellal, isoeugenol, and oak moss absolute

It is estimated that 70-80% of fragrance-allergic pa-

tients will react positively to the mix Twenty to 30%

will remain undetected, and the mix causes both false-

positive and false-negative reactions Approximately

100 fragrance materials have been identified as contact

allergens.”

to formaldehyde donors may be due either to the pre- servative itself or to formaldehyde sensitivity.21,22 Whereas the formaldehyde donors appear to be gaining

in popularity as preservatives in cosmetics, formalde- hyde itself has largely been replaced by other chemi- cals, because it is suspected (when inhaled as a gas), of being a possible human carcinogen.25

QUATERNIUM-15 (DOWICIL 2OO@) Quaternium-15 is the most frequent preservative sensitizer in cosmetic prod- ucts.’ Routine testing by the NACDG yielded a preva- lence rate of 9.6% in patients suspected of allergic con- tact dermatitis.26 Half of these reactions may be caused

by formaldehyde sensitivity.27 At the commonly used concentration of O.l%, quaternium-15 releases about

100 ppm free formaldehyde, which in some formalde- hyde-sensitive patients is sufficient to cause allergic cosmetic dermatitis In Europe, sensitization to quater- nium-15 is less frequent.28

IMIDAZOLIDINYL UREA (GERMALL 1159 Imidazolidinyl urea releases little formaldehyde, and consequently poses little threat to formaldehyde-sensitive subjects In the United States, the prevalence rate of contact allergy

to imidazolidinyl urea is 2.3%.26 Cross reactions to and from the related preservative diazolidinyl urea may be observed.28

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170 DE GROOT Clitlics in Dermnfology l 1998;16:167-179

DIAZOLIDINYL UREA (GERMALL II@) Diazolidinyl urea is

chemically related to imidazolidinyl urea It has been in

use only since 1982, and is the most active preservative

of the imidazolidinyl urea group Routine testing by the

NACDG resulted in a prevalence rate of sensitization of

3.0% in patients suspected of allergic contact dermati-

tis.z6 Diazolidinyl urea appears to be a stronger sensi-

tizer than imidazolidinyl urea Patients allergic to this

preservative may or may not react to formaldehyde.2*

2-BROMO-2-NITROPROPANE-1,3-DIOL (BRONOPOL) Bro-

nopol has been responsible for so many cases of allergic

cosmetic dermatitis from Eucerin@ cream in the United

States, that the manufacturer decided to replace it.29,30

Currently, its prevalence rate of sensitization in the

United States is 2.2%.26 Another concern is that its

interaction with amines and amides can result in the

formation of nitrosamines and nitrosamides, which are

suspected to be carcinogens In Europe, bronopol in an

infrequent sensitizer.31

DMDM HYDANTOIN Dimethylol dimethyl hydantoin

(Glydantm) has so far not been implicated as causing

cosmetic allergy, although routine testing in the United

States yielded a prevalence of 1.6% positive reactions.26

It has been demonstrated that some patients allergic to

formaldehyde may react upon patch testing to DMDM

hydantoin 32 In addition, provocation tests with a cream

containing 0.25% w/w DMDM hydantoin in formalde-

hyde-sensitive subjects elicited a positive response in

some of them, indicating that patients who are allergic

to formaldehyde may be at risk of developing allergic

cosmetic dermatitis from products preserved with

DMDM hydantoin.s2

Other Preservatives

PARABENS The paraben esters (benzyl, butyl, ethyl,

methyl, propyl) are the most widely used preservatives

in cosmetic products, and may be considered very safe

in terms of causing allergy, especially in the low con-

centrations as used in cosmetics In Europe, routine

testing with the parabens yields low rates of sensitiza-

tion (less than 1%),33 and in the United States, 2.3%.26

Most cases of sensitization to parabens are caused from

the use of topical pharmaceutical preparations on ec-

zematous skin or leg ulcers A review of paraben sen-

sitivity has been published.23

METH~(CHLORO)ISOTHIAOUNONE MI/MCI(KathonCG@,

Euxyl K loo@) is a preservative system containing, as

active ingredients, a mixture of methylchloroisothiazolin-

one and methylisothiazoli The most widely used

commercial product is Kathon CG (where CG denotes

cosmetic grade), which contains 1.5% active ingredients

In recent years, this highly effective preservative has be-

come a major cause of cosmetic allergy in most European

countries The subject has been reviewed.34,35 In the

United States, prevalence rates of 1.7%-1.9% (NACDG) and 3.6% (Mayo Clinic) have been observed.%,37 The use concentration of MCI/MI is mostly between 3-15 ppm, which is usually far below the threshold for detection of allergy with patch tests, indicating that most allergic pa- tients will not react to the product upon patch testing Therefore, MCI/MI always has to be tested separately (100 ppm water) whenever cosmetic allergy is suspected

It is present in the European Environmental and Contact Dermatitis Research Group (EECDRG) standard series Currently, MCI/MI is mainly used in rinse-off products at low concentrations, which infrequently leads to induction

or elicitation of contact allergy.38 As a consequence, prev- alence rates in Europe are decreasing

Hair Colors

Hair colors may be temporary, semipermanent, or per- manent Most cases of cosmetic allergy from hair dyes are caused by the (permanent) oxidation dyes of the PARA-type (p-phenylenediamine and related dyes) In recent years, the incidence of dermatitis due to hair dyes containing p-phenylenediamine (derivatives) ap- pears to have decreased This is attributed to the pro- vision of cautionary notices on the product, awareness

of the risk, patch testing of the product by future users, improvements in the technical quality of the cosmetic product, and improvements in the technique of appli- cation of these dyes Nevertheless, p-phenylenediamine remains an important cause of cosmetic allergy,7 6.3%

of all patients routinely tested by the NACDG reacting

to it.26 These oxidation dyes are also an occupational hazard for hairdressers and beauticians.39

Glyceyl Thioglycolate

Glyceryl thioglycolate, a waving agent used in acid permanent waving products, may sensitize consumers, but it is usually an occupational hazard for the hair- dresser.7,26,39,40 Patients allergic to glyceryl thioglycolate infrequently react to ammonium thioglycolate, used in

“hot” permanent wave procedures

Propylene Glycol

Propylene glycol is widely used in dermatologic and nondermatologic topical formulations, including cos- metics, as well as in numerous oral and parenteral medication, hygiene products, and food products.41-43

It was reported to be a common cause of cosmetic dermatitis;7 however, irritant reactions are observed regularly, and a test concentration low enough to cause

no irritation but high enough to detect all cases of sensitization is lacking

The following classification for skin reactions to pro- pylene glycol has been suggested:41 (1) irritant contact dermatitis; (2) allergic contact dermatitis; (3) nonimmu- nologic contact urticaria, and (4) subjective or sensory

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Clinics in Dermatology l 1998;16:167-179 THE SHADY SIDE OF COSMETICS 171

irritation There has been no evidence of photoallergy

or phototoxicity

Irritant reactions are common reactions, and they

should be a diagnosis of exclusion from clinical history,

physical examination, and a negative patch test

Allergic contact dermatitis is probably uncommon, and

the clinical significance has been overestimated In ear-

lier studies, higher concentrations of propylene glycol

may have induced many irritant patch test reactions

Currently, a concentration of l%-10% is advised to

avoid such irritation, but cases of contact allergy are

probably then missed (false-negative reactions).*3 A di-

agnosis of allergic contact dermatitis should never be

made on the basis of one positive patch test alone

Retesting should always be done after several weeks

Next, retests with serial dilutions down to 1% pro-

pylene glycol help in dividing irritant responses from

true allergic ones A negative reaction strongly suggests

a previously irritant response; a positive reaction sug-

gests contact allergy Repeated open application tests

(ROAT) and/or provocative use tests (PUT) can be

conducted to verify the allergic basis of a positive

patch-test result In subjects with a negative patch test,

the ROAT/PUT may also be useful as a simulation of

normal application procedures

Nonimmunolo~~ic cmtact urticaria can also occur after

topical application of propylene glycol The mechanism

entails microinjury to skin Although this does not rep-

resent a contact urticaria in the strictest sense, it is

usually categorized as such

tional Nomenclature of Cosmetic Ingredients (INCI) name: tosylamide/formaldehyde resin] The exact aller- genic ingredients of this resin are still unknown.46 Other allergens have rarely been reported: formaldehyde, ni- trocellulose (dubious), guanine, polyester resin, phtha- lates, amyl and butyl acetate, dye, ethylene dichloride, Betonite@, and o-toluenesulfonamide.45r4h

Contact allergy to toluenesulfonamide/formalde- hyde resin in nail varnish is far from rare Up to 6.6% of women habitually or occasionally using nail cosmetics and presenting with dermatitis are allergic to it,44 and the prevalence in patients routinely tested in the United States was 1.9%.26 The resin (10% petrolatum) should always be tested on subjects using nail cosmetics, be- cause neither the history nor the clinical features are sufficient criteria for excluding or suggesting the diag- nosis.44 Testing the patients’ own nail varnishes may also be helpful 14 The sociomedical consequences of nail-varnish allergy may be severe and include sick leave, hospitalization, and work loss14 Allergic patients should stop using nail varnishes or purchase varnishes free of toluenesulfonamidelformaldehyde resin (eg Shiseido, Rot, Clinique); however, apparently some products claiming not to contain the resin in fact do so.49 Also, such nail lacquers may contain other sensi- tizers, such as methyl acrylate and epoxy resin.50 Useful review articles on adverse reactions to nail cosmetics and sculptured nails have recently been published.51-53

Lanolin (Derivatives)

Subjectivebr sensory irritation, with itching, burning,

or stinging sensations but no signs of inflammation, is a

commonly noticed reaction among users of cosmetic

products and does not usually result in visits to derma-

tologists It is a phenomenon that also occurs in volun-

teers after application of propylene glycol in different

concentrations81

Toluenesulfonamidelformaldehyde

Allergens) in Nail Lacquer

Resin (and Other

Several recent articles have discussed the allergens in

nail varnish, the features of allergic contact dermatitis,

and its frequency ~44-48 Nail polish is intended to pro-

tect the nails and make them beautiful This cosmetic

product was introduced to the market in 1919; the first

reports of contact allergy date from 1925 Since then,

more than 6700 cases have been reported in the medical

literature Every second case manifests as an eyelid

dermatitis Eighty percent of all reactions are observed

as a dermatitis of the face and neck Occasionally other

parts of the body are involved, including the thighs, the

genitals, and the trunk; generalized dermatitis is rare

Periungual dermatitis may be far more common (60%)

than previously thought 14 Partner (“connubial”) der-

matitis has been observed The main allergen is the

resin toluenesulfonamide/formaldehyde resin [Interna-

Lanolin and lanolin derivatives are used extensively in cosmetic products as emollients and emulsifiers The allergens are the wool alcohols In the United States, the NACDG found a prevalence rate of 1.5% positive reac- tions in eczema patients.26 Most cases are caused by topical pharmaceutical preparations containing it, espe- cially for treating varicose ulcers and stasis dermatitis The-presence of-lanolin or its derivatives in cosmetics rarely sensitizes patients, but patients presensitized may experience cosmetic allergy from using cosmetics containing lanolin or its derivatives Avoidance of con- tact with lanolin or its derivatives often leads to disap- pearance of sensitivity.%

Stlnscreens

As drugs, sunscreens are used to prevent sunburn and

to prevent photosensitive dermatoses, such as herpes labialis and chronic polymorphic light eruption (CPLE)

In cosmetics, they are added not only to protect the skin

of the user but also to prevent the product from pho- todegradation The main classes of sunscreens are PABA and its esters (amyl dimethyl, glyceryl, octyl dimethyl), the cinnamates, the salicylates, the anthrani- lates, the benzophenones, and the dibenzoylmeth- anes.s5 The latter category is gaining popularity, be- cause it absorbs mainly in the UVA region (315-400

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172 DE GROOT Clinics in Dermatology l 2998;16:167-179

nm) UV filters have been identified with increasing

frequency as allergens and photoallergens, but reac-

tions to them remain uncommon Photoallergic reac-

tions can easily be overlooked, because the resulting

dermatitis may be interpreted by the patient/consumer

as failure of the product to protect against sunburn or as

worsening of the photodermatosis for which the sun-

screen was used PABA is a common cause of photoal-

lergic reactions Recently, most such reactions have

been caused by benzophenones and the dibenzoyl-

methanes, isopropyl dibenzoylmethane and butyl me-

thoxydibenzoylmethane 55 The literature on adverse re-

actions to sunscreens has been reviewed.55-57 The most

frequent adverse reaction to sunscreen preparations is

irritation, which may occur in over 15% of users.58 Both

allergic and photoallergic reactions are reported to the

main classes of UVA filters: benzophenones and diben-

zoylmethanes 10~9-63 Oxybenzone (benzophenone 3) es-

pecially causes many cases of photoallergic contact der-

matitis, and to a lesser extent allergic contact

dermatitis.10,59-61,63 Patients who regularly use sun-

screens because they suffer from the photosensitivity

dermatitis/actinic reticuloid syndrome may have an

increased risk for developing allergic side effects of

sunscreens.6:’ In all cases of suspected adverse reactions

to sunscreens, both patch and photopatch tests should

be performed.‘O

New Important Cosmetic Allergens

New important contact allergens do not appear every

day; however, in recent years two chemicals, the sur-

factant cocamidopropyl betaine and the preservative

methyldibromo glutaronitrile (in Euxyl@ K 400), have

emerged as important causes of allergic cosmetic der-

matitis Tocopheryl linoleate, a vitamin E derivative

present in a new line of cosmetics, was the cause of an

epidemic of contact dermatitis; the products were hast-

ily withdrawn from the market

Cocamidopropyl Befaine

Cocamidopropyl betaine is an amphoteric surfactant

that enjoys increasing popularity among cosmetic

chemists because of its low potential for irritation of the

skin.‘b-18 In the United States, the surfactant in 1980 was

present in only 47 of 19,000 products on file with Food

and Drug Administration (FDA), in 1992 this number

had risen to 521.3 Most of the products are shampoos

and bath products, such as bath and shower gels

Cocamidopropyl betaine is prepared by reacting co-

conut fatty acids (obtained from coconut oil) with di-

methylaminopropylamine, yielding cocamidopropyl di-

methylamine, which is subsequently allowed to react

with sodium monochloroacetate to give the end prod-

uct cocamidopropyl betaine The lipophilic tail is

formed by coconut fatty acids, a mixture of fatty acids

with chain lengths varying between C, and C,, Coca- midopropyl betaine is therefore a mixture of several compounds with the same basic structure, but with differing lipophilic tails Depending on the source, co- camidopropyl betaine can still contain varying amounts

of the reactants and intermediates involved in its syn- thesis A major impurity is dimethylaminopropylamine Case Reports of Contact Allergy to

Cocamidopropyl Betaine The first case of cosmetic allergy to cocamidopropyl betaine was reported in 1983 In many cases published since then, shampoo was the causative cosmetic prod- uct Thus, cocamidopropyl betaine is the exception to the rule that allergic reactions are usually caused by

“stay-on” (“leave-on”) cosmetic products Other cos- metic products that have caused cosmetic allergy from the presence therein of cocamidopropyl betaine include skin-care products (moisturizing and cleansing prod- ucts), deodorant, shower gel, bath foam, and liquid soap; in addition, several cases of contact allergy to cocamidopropopyl betaine in contact lens fluids have been described

Epidemiology of Contact Dermatitis to Cocamidopropyl Betaine

By its presence in shampoos, cocamidopropyl betaine appears to be an important occupational hazard to hair- dressers Prevalence rates of sensitization to cocamido- propyl betaine range from 3.7% to 5% One investigator obtained 12 positive patch test reactions among 210 (5.7%) patients suspected to suffer from cosmetic-re- lated allergic contact dermatitis and/or dermatitis of the head and neck area.64 Seven of these were consid- ered definitely relevant All of these were allergic to shampoos containing cocamidopropyl betaine.@ A group of investigators tested 1200 consecutive patients with dermatitis of various types with cocamidopropyl betaine and found allergic reactions in 46 (3.8%).65 Patch tests were relevant in all, the causative products being mostly shampoos and bath foam.65 In another Italian study, 17 of 1190 unselected eczema patients (1.4%) proved to be allergic to cocamidopropyl be- taine.66 The products causing allergic contact dermatitis were shampoos, face cleansing lotions, gynecological antiseptic syndets, liquid shower soaps, and anal hy- gienic detergents 66 In Arnhem, the Netherlands, 56 out

of a population of 781 dermatitis patients (7.2%) reacted

to cocamidopropyl betaine; however, in only 17 of these

56 (30%, ie 2.2% of the population tested), were the reactions scored as relevant.17

The Allergen(s) in Cocanzidopropyl Betaine Depending on its source, cocamidopropyl betaine con- tams varying amounts of the reactants and intermedi- ates involved in its synthesis To determine the actual allergenic ingredient in cocamidopropyl betaine, a

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Clinics in Dernzatolqpy l 1998;16:167-179 THE SHADY SIDE OF COSMETICS 173

group of investigators tested 30 patients allergic to co-

camidopropyl betaine with the chemicals used for its

synthesis 65 All reacted to dimethylaminopropylamine

1% aqua, whereas only 16 patients (53%) reacted to

cocamidopropyl betaine of purer grade.65 The authors

suggest that dimethylaminopropylamine is the (only)

allergen in cocamidopropyl betaine.65 These results

were later confirmed.66 Thus, it would appear that dim-

ethylaminopropylamine is the major allergen in coc-

amidopropyl betaine and other alkylamidobetaines.66,67

As the amounts of dimethylaminopropylamine found

in commercial preparations containing cocamidopropyl

betaine is far lower than the eliciting concentration, this

concept may be challenged.68

Patch Testing With Cocamidopropyl Betaine

Cocamidopropyl betaine usually causes allergic reac-

tions in rinse-off products As patch testing with these

products is likely to result in both false-positive and

false-negative reactions, and such procedures therefore

are often unreliable, it is imperative that cocamido-

propyl betaine be tested separately It is therefore

suggested to include cocamidopropyl betaine in the

hairdresser’s series and in the cosmetic series Coca-

midopropyl betaine in the usual and commercially

available concentration of 1% in water is a marginal

irritant, and not all “positive” patch test reactions indi-

cate (relevant) contact allergy to it Relevance can be

established only when allergic patients are actually ex-

posed to products containing cocamidopropyl betaine,

and avoidance results in clearing or obvious improve-

ment of dermatitis

Mefhyldibromoglutaronitrile (EuxyP K 400)

Euxyl K 400 (Schiilke & Mayr, Norderstedt, Germany)

is a preservative system for cosmetics and toiletries,

containing 2 active ingredients: methyldibromoglut-

aronitrile (MDG; synonym: 1,2-dibromo-2,4-dicyanobu-

tane) and phenoxyethanol in a 1:4 ratio.ls-20 Typical use

concentrations are 0.05%-0.20% Euxyl@ K 400 was in-

troduced in Europe in the mid 198Os, and it has been

used in the United States for approximately 6 years In

the Netherlands in 1995,25%-35% of all cosmetic prod-

ucts and two-thirds of all moistened toilet tissues used

for anal hygiene were preserved with Euxyl K 400 In

the United States in 1992, methyldibromoglutaronitrile

was present in only 7 out of 20,000 products on file with

the FDA.” In 1995, this number had risen to 56 of 22,287

products Methyldibromoglutaronitrile is also available

for a variety of noncosmetic (industrial) applications.69

Contact Alleugj to Euxyl K 400 and its Ingredient

Methyldibromoglutavollitrile

The sensitizer in Euxyl K 400 is nearly always methyl-

dibromoglutaronitrile The first cases of contact allergy

to Euxyl K 400 were reported from Germany in 1989

Later case reports include sensitization to moistened

toilet tissues (especially in the Netherlands), skin-care products, cucumber eye gel, barrier cream gel, cleans- ing cream, ultrasonic gel, and makeup.19 In the Nether- lands, the prevalence of contact allergy to methyldibro- moglutaronitrile rose from 0.5% in 1991 to 4.0% in 1994.20,70 It is now the most frequent cosmetic allergen

In Bologna, Italy, the prevalence of allergy to Euxyl K

400 rose from 1.2% in 1988-1990 to 2.3-2.9% in 1991- 1994.71~7z In Germany, approximately 2.3% of suspected contact dermatitis patients are currently allergic to Eu- xyl K 400 and the members of the North American Contact Dermatitis Group in 1992-1994 found a preva- lence of 1.5%.26,73 Between 23% and 75% of positive patch-test reactions are considered to be relevant Usu- ally, cosmetics, both of the stay-on and of the rinse-off variety, and, in the Netherlands, moistened toilet tis- sues, were the cause of the reaction.2”

The Profile of Patients Sensitized to Euxyl K 400

Allergic patients have either cosmetic dermatitis or perianal eczema The former category are usually women, the latter mainly men Cosmetic dermatitis is often localized on the face and/or periorbital and on the neck Skin-care products used for prevention and/or treatment of dry skin may also be the cause of cosmetic dermatitis, which may thus be localized, especially on the hands, but also on the arms, the neck; widespread;

or even generalized Occupational hand dermatitis may

be observed in hairdressers and masseurs

The Appropriate Test Concentration

Most investigators have used Euxyl K 400 “per se” as test allergen As the allergen in Euxyl K 400 is nearly always its ingredient methyldibromoglutaronitrile, MDG can thus be conveniently utilized as a single allergen indicator for allergy to Euxyl K 400.74 The optimal test concentration (and vehicle, MDG has thus far been tested only in petrolatum) has yet to be estab- lished, but it may be 0.5% in petrolatum In our expe- rience, it is necessary to add an emulsifier, such as soy lecithin, to obtain homogeneous dispersions With the currently available commercial allergens, methyldibro- moglutaronitrile 0.1% pet (Trolab) and Euxyl K 400 0.5% pet (Chemotechnique), false-negative reactions may occur.74

Folliculav Reactions fo Tocopheryl Linoleafe

in Cosmef its

In spring 1992, an epidemic outbreak of skin eruption caused by a new line of cosmetics occurred throughout Switzerland.75,76 Within a 3-month period, this outbreak affected at least 263 people who consulted dermatolo- gists and at least 642 people who did not The lesions were mainly papular and follicular, widely distributed, with pronounced pruritus, which was aggravated by sweating or heat exposure, and were long lasting Epi- demiological and clinical data incriminated a new line

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Clinics in Dermatology 1998;16:167-179

of cosmetics containing vitamin E linoleatem, a mixture allergy from nail lacquers or hardeners A thorough

of fatty acid esters of DL-a-tocopherol composed not history of cosmetic usage should always be obtained It only of tocopheryl linoleate but also of tocopheryl must be stressed that most women think of “cosmetics” oleate, palmitate, and myristate The lesions appeared in terms of decorative cosmetics such as eye shadow, after l-160 days (mean 14 days) following the initial rouge, lipsticks, hair colors, and nail lacquers There- application of the tocopheryl linoleate-containing cos- fore, it is necessary to specifically inquire about prod- metics In many cases, the rash extended and the pru- ucts such as day and night cream, cleansers, makeup ritus increased several days after the application had removing pads, perfume, eye cream, and so forth The been stopped In l/5 of the cases, a secondary extension “classic” cosmetic dermatitis of periorbital dermatitis

to the face was seen, though the cosmetics had not been may be caused by any product used on the hair, the applied to these sites scalp, the face, the hands, and the nails

Patch testing showed positive patch tests with the

undiluted final cosmetic products, ranging from 21% to

64%, depending on the individual product None of the

control subjects showed a positive test reaction In ad-

dition, 60% of patients had positive or doubtful reac-

tions to undiluted vitamin E linoleate, while none of

controls had Thirteen out of 45 (29%) tested patients

presented a positive or doubtful reaction to tocopheryl

linoleate 10% in petrolatum Patch tests with other vi-

tamin E derivatives induced only a few positive reac-

tions Twelve of 15 (80%) patients who performed re-

peated open-application tests reacted to the body lotion

containing tocopheryl linoleate; however, several also

reacted to the lotion without tocopheryl linoleate

Skin biopsies performed on lesional skin showed

spongiosis of the follicular epidermis with a perifollic-

ular and perivascular infiltrate containing a predomi-

nantly mononuclear clear cell infiltrate with some neu-

trophils An in vitro time-dependent formation of

oxidative products under storage or oxidation-stimulat-

ing conditions was observed The authors conclude that

oxidized vitamin E derivatives could act in vivo as

haptens and/or irritants, possibly with synergistic ef-

fects.75 An allergic mechanism was later favored by the

authors.76

This is a unique study of a very curious cosmetic

reaction The mechanism remains unknown Several

factors favor an allergic etiology, others suggest irrita-

tion rather than contact allergy Probably more than one

mechanism was involved However, there can be no

doubt that tocopheryl linoleate was the cause of this

outbreak of cosmetic contact dermatitis

Diagnostic Procedures

The diagnosis of cosmetic allergy should strongly be

suspected in any patient presenting with dermatitis of

the face, eyelids, lips, and neck.7L78 Cosmetic allergic

dermatitis may develop on previously healthy skin of

the face or on already damaged skin (irritant contact

dermatitis, atopic dermatitis, seborrheic dermatitis,, al-

lergic contact dermatitis from other sources) Also, der-

matitis of the arms and hands may be caused or wors-

ened by skin-care products to treat or prevent dry skin

and irritant or atopic dermatitis Patchy dermatitis in

the neck and around the eyes is suggestive of cosmetic

When the diagnosis of cosmetic allergy is suspected, patch tests should be performed to confirm the diagno- sis and identify the sensitizer Only in this manner can the patient be counseled about future use of cosmetic (and other) products and the prevention of recurrences

of dermatitis from cosmetic or noncosmetic sources Patch tests should be performed with the NACDG or EECDRG routine series, a “cosmetic series” containing known cosmetic allergens, and, of course, all products used by the patient The NACDG routine series con- tains a number of allergens that may cause allergic cosmetic dermatitis: rosin (colophony, an indicator for perfume allergy, and a possible allergen in eyeshadow); the preservatives diazolidinyl urea, imidazolidinyl urea, formaldehyde and quaternium-15; the fragrances cinnamic aldehyde and cinnamic alcohol and balsam of Peru (indicator for perfume sensitivity); the hair color p-phenylenediamine; and lanolin alcoho1.26 The EECDRG routine series contains colophony, balsam of Peru, the fragrance mix, formaldehyde, quaternium-15, methyl- (chloro)isothiazolinone, wool alcohols, and p-phenyl- enediamine A suggested “cosmetic series” is shown in Table 1 Most of these allergens are available from Chemotechnique (Malmo, Sweden) or from Hermal Chemie (Reinbek/Hamburg, Germany) Although the patient’s products should always be tested (for test concentrations, see Table 2), patch testing with cosmet- ics has some important drawbacks Patients often use many cosmetic products, which makes the investigation very laborious More importantly, both false-negative and false-positive reactions occur frequently False-neg- ative means that the patient is allergic to a certain cosmetic, but the patch test reaction to the product itself remains negative This is due to the low concentration

of some allergens and the usually weak sensitivity of the patient The product does cause allergic cosmetic dermatitis when applied repeatedly (eg daily), when applied to damaged (dry or eczematous) skin, or when applied to very sensitive skin, for example, the eyelids One application on the thick and intact skin of the back, even under occlusion (as is the case with patch testing)

is insufficient to cause a positive patch test reaction Classic examples of false-negative reactions are with methyl(chloro)isothiazolinone and paraben sensitivi- ty.34,“6 Therefore, such allergens have to be routinely

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Clinics in Dermatology l 1998;16:167-2 79 THE SHADY SIDE OF COSMETICS 175

Table 1 Suggested Allergens for a “Cosmetic Screening Series”

Allergen Function

Test Concentration and Vehicle

Amerchol L lOl*

Benzophenone-3 (oxybenzone) Benzophenone-10 (mexenone) BHA (butylated hydroxyanisole) BHT (butylated hydroxytoluene) 2-Bromo-2-nitropropane-1,3-diol Cetearyl alcoholt Cocamidopropyl betaine Diazolidinyl meaS Fragrance mix§

Glyceryl thioglycolate Imidazolidinyl urea$

Isopropyl dibenzoylmethane Methyl(chloro)isothiazolinone§

Methyldibromo glutaronitrile Octyl dimethyl PABA PABA

Parabenss Propolis Propylene glycol Toluenesulfonamide/formaldehyde resinq[

Emulsifier Sunscreen Sunscreen Antioxidant Antioxidant Preservative Emulsifier Surfactant Preservative Fragrance Permanent waving agent Preservative

Sunscreen Preservative Preservative Sunscreen Sunscreen Preservatives Natural ingredient Humectant Nail lacquer resin

50% pet 2% pet 2% pet 2% pet 2% pet 0.5% pet 30% pet 1% water 2% water or pet 8X1% pet 1% pet 2”%i pet 2% pet

100 ppm in water 0.5% pet 2% pet 2% pet 5X3% pet

10% pet 10% water 10% pet

pet = petrolatum

* INCl name: innolin alcohol and paraffinurn liquidurn

f 1NCl nnme: cetyl alcohol, stearyl alcohol

#Present in the NACDG series

5 Present in the EECDRG series

y TNCJ name: tosylamide/formaldehyde resin

tested in the appropriate concentration in a cosmetic

screening series False-positive reactions may occur

with any cosmetic product, but especially with prod-

ucts containing detergents/surfactants such as sham-

poo, soap, bath, and shower foam As a consequence,

these products are usually diluted to 1% in water before

testing Even then, mild irritant reactions are observed

frequently; and, of course, the (necessary) dilution of

Table 2 Recommended Test Concentrations for Cosmetic

Products

Cosmetic Product Test Concentration and Vehicle

Depilatory Thioglycolate 1% pet

Foaming bath product 1% Water

Foaming cleanser 1% Water

Mascara Pure (allow to dry)

Nail cuticle remover Individual ingredients

Nail glue Individual ingredients

Nail polish Pure (allow to dry)

Nail polish remover Individual ingredients

Permanent wave solution Glyceryl thioglycolate 1% pet

Shaving lather or cream 1% Water

Skin lightener Hydroquinone 1% pet

Soap or detergent 1% Water

Straightener Individual ingredients

Adapted from De Groat, Weijlmd and N&r.”

these products may result in false-negative results in patients who are actually allergic to them Testing these products, therefore, is highly unreliable In many cases, testing with the NACDG/EECDRG routine series, the suspected products, and a cosmetic screening series will establish the diagnosis of cosmetic allergy and identify one or more contact allergens On the incriminated product or the label can be found whether or not the product actually contains the allergen(s) If not, the possibility of a false-positive reaction to the product should be suspected The test should be repeated and/

or control tests on nonexposed individuals should be performed If allergy is confirmed, an ingredient of the product that was not tested in the NACDG/EECDRG series and the cosmetic screening series may have been responsible In such cases, the manufacturer should be asked for samples of the ingredients, and these can be tested on the patient after proper dilution.79

In certain cases, allergy to cosmetics is strongly sus- pected, but patch testing remains negative In such patients, ROAT and/or usage tests can be performed

In the ROAT, the product is applied twice daily for a maximum of 14 days to the antecubital fossa A nega- tive reaction after 2 weeks makes sensitivity highly unlikely This procedure should be performed with all suspected products In the usage test, all cosmetic prod- ucts are stopped until the dermatitis has disappeared Then, cosmetics are reintroduced as normally used, one

at a time, with an interval of 3 days for each product,

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Clinics in Dermatology l 1998;16:167-179

until a reaction develops Diagnostic problems with

propylene glycol were discussed in a previous section

Photopatch testing should be performed whenever

photoallergic cosmetic dermatitis is suspected When

all tests have remained negative, the possibility of

seborrheic dermatitis (scalp, eyelids, face, axillae,

trunk), atopic dermatitis (all locations), irritant contact

dermatitis (also from cosmetic products), and allergic

contact dermatitis from other sources should first

be considered

Therapy and &even tion

The therapy of allergic cosmetic dermatitis consists of

discontinuation of the (suspected) allergenic product(s)

and, if necessary, topical (and rarely systemic) steroids

To prevent recurrences, the patient should receive the

Cosmetic, Toiletry and Fragrance Association (CTFA)

names (United States) or INCI names (European Union)

of the allergen(s) identified, and be instructed to avoid

cosmetic and non-cosmetic products containing them

and possible cross-reacting (chemically-related) sub-

stances Cosmetic ingredient labelling enables the pa-

tient to choose products not containing these In the

case of contact allergy to fragrances, balsam of Peru and

possibly colophony, unfragranced products should be

used In some patients a fragrance may sometimes be

applied to clothing or hair without eliciting an allergic

response “Connubial contact” (ie from the partner)

with fragrances should be avoided.6 Many allergens in

cosmetics are relatively easy to avoid, because they are

used only or mainly in cosmetics Others have many

applications (eg methyl(chloro)isothiazolinone), and

some are impossible to avoid (eg formaldehyde)

Finally! Ingredient Labelling in the European

Union New Opportunities but Also

New Problems

Cosmetic ingredient labelling (introduced in the United

States already 20 years ago!) has been a constant de-

mand of European dermatologists and allergists for

years 12,80,8* The benefits are obvious: dermatologists

have a better chance to identify allergens in products

used by their patients; and, if a patient is allergic to one

or more cosmetic ingredients, he or she can avoid prod-

ucts containing this ingredient

On January lst, 1997, the 6th Amendment to the

European Union Cosmetics Directive (76/768) came

into force This directive requires, among others, that all

cosmetic products marketed in the European Union

display their ingredients on the outer package or, in

certain cases, on an accompanying leaflet, label, tape, or

tag.82 The primary purpose of ingredient labelling is to

allow dermatologists to identify specific ingredients

that cause allergic responses in their patients and enable

such patients to avoid cosmetic products containing the

substances to which they are allergic by checking their labels.l* The nomenclature used throughout the Euro- pean Union for labelling is the INCI (International No- menclature Cosmetic Ingredient), based on the (Amer- ican) CTFA (Cosmetic, Toiletry and Fragrance Association) nomenclature Most CTFA terms have been retained unchanged All colorants are listed as color index (CI) numbers, except hair dyes, which have INCI names Plant ingredients are declared as genus/ species using the Linnaean system The source of infor- mation on ingredients is the European Inventory pub- lished in all official European Union languages Provided are the INCI names (in alphabetical order), CAS-number, EINECS-ELINCS numbers, chemical/IU- PAC names, and functions EINECS = European inven- tory of existing commercial chemical substances and ELINCS = European list of notified chemical sub- stances

We have found that the inventory has several disad- vantages The major problem is the “translation” of plant products and colors from the CTFA nomenclature

to the INCI Lists of synonyms are not provided Only those who have access to botanical literature and spe- cific literature on colors can find relevant names Apart from the fact that we will have to get used to some very exotic names, who would be able to find “Myroxylon Pereirae” for balsam of Peru, “Eugenia caryophyllus” for clove oil or “CI 77000” for aluminum?

The order of listing is sometimes rather illogical: for p-aminophenol, look under “pa .” instead of “Am .“ Benzophenone-11 comes before benzophenone-2 (be- cause 11 begins with 1, thus lower than 2)

It is stated that fragrances have not been included in the INCI, because they need not to be declared; how- ever, we found many fragrance names (eg geraniol, hydroxycitronellal, cinnamal, cinnamyl alcohol) Their function is described as “additives” Additives are de- fined as “Substances which, often in fairly small amounts, are added to cosmetic products to create or improve desired properties or minimize or suppress undesired properties” In this context one may think of

“masking perfumes”, the classic example of which is ethylene brassylate (indeed mentioned in the invento- ry) We do not know whether producers of cosmetics will actually declare such fragrances on the label In addition, dermatologists will have to check the inven- tory as to whether or not a specific fragrance is included before advising patients allergic to these individual fragrance compounds

In spite of the fact that fragrances do not need to be declared, part II of the inventory lists some 2500 fra- grances (including plant extracts) and aroma chemicals

In itself this could be very useful; however, the chemi- cals are not listed in alphabetical order, but in order of ascending EINECS/ELINCS numbers, and thus impos- sible to trace for almost all dermatologists

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