Molecular analysis of maternal diabetes induced changes in the developing neural tube

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Fig 1

B

III

LV

e

dc vt dt

vtw

E

e

dc

vt dt

vtw

Fig 2

0

50

100

150

200

250

300

ControlDiabetic

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A B

Fig 4

0

20

40

60

80

100

120

140

160

180

Control Diabetic

*

Fig 5

A

dc

vt

B

dc vt

e

0

0.5

1

1.5

2

2.5

3

3.5

Control Diabetic

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B

333bp

C

Fig 7

III

LV

dc

vt

dc vt

Fig 8

dc

vt

dc

vt e

III

LV

vt LV

III

B Fig 10

0

0.5

1

1.5

2

Control Diabetic

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B

325bp

C

e dc

vt

dt

vt

dmt

*

Fig 12

0.2

0.4

0.6

0.8

1

1.2

1.4

Control Diabetic A

B

238bp

C

A B C

Fig 14

C

LV LV

ctx

cn III

III

G

III III

E

D

LV LV

III

A

III

sn

hi

bt

H

III

F B

5

10

15

20

25

30

35

40

45

Normal Diabetic CP-treated CP-treated diabetic

**

Fig 16

III

III

III

A

III

C

E

D 5A

F G

III

III

chp hi sn

dn

bt

III

tm

htms htm

III

B

H

TGF-ß l TGF-ß l

TGF-ß1 TGF-ß l

TGF-ß l

TGF-ß l

A B C

III

D

TGF-ß1

TNF-α

Lectin

Lectin

TGF-ß1+Lectin

TNF-α+Lectin

Fig 18

B

III

tm

htm s

htm

A

10

20

30

40

50

CP-treated diabetic

TNF- α TGF-β1 TGF-β1 mRNA

*

*

*

Fig 20

iNOS TGFß IL-1ß

TNFα

215bp

241bp

173bp

370bp

A

B

C

D

0.51

1.5

2

2.53

3.54

4.55

5.56

6.5

7

7.58

Normal Diabetic CP-treated CP-treated diabetic

**

**

Fig 22