Mass spectrometry analysis was done in collaboration with Christopher Stephen Hughes and Prof Gilles Lajoie.. * Protein also found in GFR and standard Matrigel, based on work by Hughes e
Trang 1DxSDOC DxSDOCDOC
Subcellular
Location
Accession # Molecular
Weight (Da)
Protein Name Identification
Probability
# of Unique Peptides
% Coverage
Protein name Identification
Probability
# of Unique Peptides
% Coverage
Cytoplasm IPI00216587,
IPI00021439,
IPI00894365,
IPI00894498
IPI00025416 41860 Actin, gamma-enteric
IPI00013452 170575 Bifunctional
IPI00152692 23406 D-tyrosyl-tRNA(Tyr)
deacylase 1
deacylase 1
IPI00396485,
IPI00472724
50123 Elongation factor
1-alpha 1*
1-alpha 1*
IPI00297982 51092 Eukaryotic translation
initiation factor 2 subunit 3
IPI00219018,
IPI00789134,
IPI00795257
36035
Glyceraldehyde-3-phosphate dehydrogenase*†
Glyceraldehyde-3-phosphate dehydrogenase*†
IPI00414676 83249 Heat shock protein HSP
IPI00141318,
IPI00604713,
IPI00883950,
IPI00939370
Cytoskeleton-associated protein 4*†
Cytoskeleton-associated protein 4*†
IPI00003865,
IPI00037070,
IPI00939595
70882 Isoform 1 of Heat shock
cognate 71 kDa protein*†
Trang 2IPI00019502 226520 Isoform 1 of Myosin-9* 100.00% 16 12.90% Isoform 1 of
IPI00302592,
IPI00333541,
IPI00644576
Filamin-A*
IPI00043863,
IPI00220113,
IPI00396171,
IPI00411375,
IPI00743873,
IPI00878314,
IPI00878651,
IPI00880007,
IPI00888475,
IPI00924603,
IPI00927173,
IPI00927819
Microtubule-associated protein 4
IPI00465248,
IPI00759806 47152 Isoform alpha-enolase of Alpha-enolase* 99.80% 2 6.68%
IPI00479186,
IPI00847989 57920 Isoform M2 of Pyruvate kinase isozymes
M1/M2*
IPI00000105,
IPI00910019 99308 Major vault protein*
IPI00008868 270602 Microtubule-associated
IPI00166768,
IPI00180675,
IPI00218343,
IPI00387144,
IPI00792677,
IPI00793930,
IPI00909762,
IPI00930688
Trang 3IPI00220967,
IPI00744135,
IPI00856098
Ribosome-binding protein 1
cytoskeletal 10
Extracellular
IPI00304962 129297 Collagen alpha-2(I)
IPI00329573,
IPI00964552 333127 Isoform 1 of Collagen alpha-1(XII) chain 100.00% 23 11.80% Isoform 1 of Collagen alpha-1(XII) chain 100.00% 21 10.10% IPI00022200,
IPI00072918,
IPI00220701
343645 Isoform 1 of Collagen
IPI00022418,
IPI00339223,
IPI00339224,
IPI00339225,
IPI00339226,
IPI00339227,
IPI00845263,
IPI00855785
IPI00031008,
IPI00220213 240832 Isoform 1 of Tenascin*
Transglutaminase 2
Transglutaminase 2
IPI00304840 108563 Isoform 2C2 of Collagen
alpha-2(VI) chain
Trang 4metalloproteinase with thrombospondin motifs 1*† IPI00018219,
IPI00873923,
IPI00940091
factor-beta-induced protein ig-h3
Membrane IPI00022143,
IPI00746655
122841 Isoform 1 of Extended
IPI00021048,
IPI00216268,
IPI00216269,
IPI00645867
IPI00219365,
IPI00021405,
IPI00216952,
IPI00644087,
IPI00655812
74123 Isoform A of
differentiation-associated protein AHNAK*†
IPI00646304 23725 Peptidyl-prolyl cis-trans
isomerase B*†
IPI00018534,
IPI00020101,
IPI00152906,
IPI00303133,
IPI00329665,
IPI00419833,
IPI00477495,
IPI00554798,
IPI00646240,
Trang 5IPI00794461,
IPI00815755,
IPI00930174,
IPI00930570
Unknown IPI00022431,
IPI00795830,
IPI00922262,
IPI00953689
46608 cDNA FLJ55606, highly
similar to Alpha-2-HS-glycoprotein
highly similar to Alpha-2-HS-glycoprotein
IPI00376689 152983 Isoform 1 of Protein
KIAA1199
IPI00022434,
IPI00745872,
IPI00966829
71687 Putative uncharacterized
protein ALB
uncharacterized protein ALB
Table 2: Consolidated list of protein identifications compiled from mass spectrometric analysis of DxSDOC and DxSDOCDOC matrices Mass spectrometry analysis was done in collaboration with Christopher Stephen Hughes and Prof Gilles Lajoie
* Protein also found in GFR and standard Matrigel, based on work by Hughes et al 2009
† Murine protein is a homolog of human protein