T cell responses in helicobacter pylori associated gastroduodenal diseases 2

63 Figure 3.4 Pro-inflammatory cytokine and chemokine profile in gastric environment.. A-C Semi-quantitative SYBR-Green real time PCR for CCL20, hBD-2 and IL-23p19 mRNA respectively.. Ge

63

Figure 3.4 Pro-inflammatory cytokine and chemokine profile in gastric environment (A-C) Semi-quantitative SYBR-Green real time PCR for CCL20, hBD-2 and IL-23p19 mRNA respectively Gene expression was normalized to the housekeeping gene β-actin and arbitrary values were further normalized to group N (D) Semi-quantitative TaqMan Gene expression assay of IL-1β mRNA Gene expression was normalized to the housekeeping gene GAPDH and arbitrary values were

further normalized to group N (E-G) Milliplex xMAP assay for ex vivo IL-6, TNF-α and IL-1β concentrations (H) ELISA for ex vivo hBD-2 concentrations (I-L) Subclassification of group P individuals into PC+ or PC- groups for analysis of ex vivo concentrations of IL-6, TNF-α, IL-1β and hBD-2 respectively

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Figure 3.5 Other pro-inflammatory cytokines and chemokines analysed Milliplex xMAP assay for ex vivo IL-1α, IL-1Ra, IL-2, IL-8, IL-10, IL-12p40, IL-15,

MCP-1 and RANTES concentrations