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Gas chromatographyColumn Temperature program Detector Injector and detector temperatures Carrier gas Sample size... measuring, analyzing volatile composition without introducing artifa

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quan

MÀU S Ắ C

Tính ch ấ t c ả m quan

Màu vàng: beta Carotene

Màu xanh: Chlorophyl

Agar

…

Copaene

…

Gas chromatography

Column Temperature program Detector

Injector and detector temperatures

Carrier gas Sample size

Fig 1 Gas chromatogram

25

24

31 28

41 37

36

32

50 45

61 57

55

65 67 68 64

63 62

DỮ LIỆU TỪ THIẾT BỊ

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Gas chromatography/ Mass spectrometry

Fig 1 Gas chromatogram

25

24

31 28

41 37

36

32

50 45

61 57

55

65 67 68 64

63 62

measuring, analyzing volatile composition without

introducing artifacts

GC/O: GC associated with human

nose as a detector is performed as GC/O analysis

Human response and detector response are made simultaneously at a given retention time

Equipment for GC-sniffing analysis

Humid air

Air compressor

Injection

Sniffing

Recorder

Gas chromatography/Olfactometry

The major GC/O differ essentially in the way they determine the aroma potency of individual

compounds.

Dilution method: AEDA and CHARM

Intensity method: OSME and GC/O-FSM

Odor detection frequency method

[odorant in the last dilute extract]

The primary difference between AEDA and CHARM:

CHARM measures the dilution value over the entire time,

AEDA determines the maximum dilution value detected.

The extract is only injected at a single dilution level

Odor intensity recorded as a function of time by moving the cursor of resistors

Odor detection frequency

method

Based on the response of the whole panel by pressing a button during

the entire sensory impression, resulted in square signals

Total responses are summed, then NIF, peak height and SNIF, peak

area are obtained as indicators of an odor

time (min)

Nasal impact frequency (NIF)

100 %

Surface of NIF (SNIF)

NIF = 100%, the odorant was detected by

all panelists.

FD factor: The essential oil was stepwise diluted 3-fold with acetone,

the highest dilution of individual component detected by assessor was defined as its FD factor

Relative flavor activity:

Aromagram

Gas chromatogram (top) and aromagram (bottom)

7 10

6 8

10

14 13 16

29 27

39

37 36 34

55 54

3 4

23 19

55

68

65 64

Volatile components

17.6 5

Green

* Octyl acetate

22

1.0 9

Pungent, green, sour 20.2

Green 0.1

Sweet, citrusy 0.1

Fresh, fruity 0.2

Pungent, green, lemon-like 70.5

Limonene

10

RFA FD

Odor description w/w (%)

Compound

No

Chiral analysis

PA

1

2 3

Chiral analysis of citronellal

Sweet, turpentine-like nd

Kabosu aroma model from (R)-(+)-citronellal

0.25%, smelling strip

0.016%, headspace-sniff

30 mL glass brown vial