Vi nấm (Fungi) và độc tố vi nấm (Mycotoxin) là vấn đề rất quan trọng trong công tác bảo quản nông sản, thực phẩm và trong y tế. Gần 400 độc tố vi nấm được phát hiện cho đến nay [18], trong đó, aflatoxin là độc tố được quan tâm nghiên cứu nhiều nhất
Trang 1Due to high toxicity, the permitted limit of aflatoxin in comodity is very low (1 – 10 ppb) Physico-chemical methods have some backdraws, such as the necessity of using much superpure organic solvents The method using aflatoxin-specific antibodies conjugated on the Sepharose CL – 4B in IAC (Immunoaffinity Column) allows simultaneously purify and concentrate aflatoxin IAC can be used with 2 purposes: (a) replace the necessary extraction step before using physico-chemical methods (TLC, HPLC) (b) directly isolate and quantify aflatoxin with fluorescent method The composition was aimed to identify two technical properties of the Pasteur Institute’s IAC to AFG1 The sensibility and repeatability were determined:
The sensibility: 1 ppb
The repeatability: CV= 7,8% and 8,03% with AFG1 amounts of 4 ng and 19 ng respectively Those are statistically acceptable
Conclusion: the IAC is good for the analysis of AFB1 as well as AFG1
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