Với mong muốn phát triển hơn nữa khả năng nuôi trồng và ứng dụng nấm vân chi trong ngành dược phẩm, chúng tôi thực hiện đề tài: Khảo sát sinh trưởng một chủng nấm vân chi đen Trametes versicolor có nguồn gốc từ Trung Quốc.
Trang 1PHỤ LỤC
PHỤ LỤC 1: Kết quả phân tích thống kê các thí nghiệm môi trường nhân giống cấp 1
One-Way Analysis of Variance
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Data: THDDUONG.ketqua
Level codes: THDDUONG.nt
Labels:
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
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Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 953.80467 4 238.45117 48.819 0000
Within groups 48.84405 10 4.88441
-
Total (corrected) 1002.6487 14
0 missing value(s) have been excluded
Table of means for THDDUONG.ketqua by THDDUONG.nt
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Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
psa 3 79.235000 1.2928554 1.2759839 77.224110 81.245890 cra 3 60.220000 3878574 1.2759839 58.209110 62.230890 bth 3 78.113333 1.7888202 1.2759839 76.102444 80.124223 dua 3 82.806667 7798789 1.2759839 80.795777 84.817556 gia 3 78.793333 1.5845118 1.2759839 76.782444 80.804223 -
Total 15 75.833667 5706374 5706374 74.934369 76.732964 Multiple range analysis for THDDUONG.ketqua by THDDUONG.nt
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Method: 95 Percent LSD
Level Count Average Homogeneous Groups
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cra 3 60.220000 X
bth 3 78.113333 X
gia 3 78.793333 XX
psa 3 79.235000 XX
dua 3 82.806667 X
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contrast difference limits
psa - cra 19.0150 4.02178 *
psa - bth 1.12167 4.02178
psa - dua -3.57167 4.02178
psa - gia 0.44167 4.02178
cra - bth -17.8933 4.02178 *
cra - dua -22.5867 4.02178 *
cra - gia -18.5733 4.02178 *
bth - dua -4.69333 4.02178 *
bth - gia -0.68000 4.02178
dua - gia 4.01333 4.02178
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* denotes a statistically significant difference
Trang 2
One-Way Analysis of Variance
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Data: THNHDO.ketqua
Level codes: THNHDO.nt
Labels:
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
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Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 51.861600 1 51.861600 7.880 0485
Within groups 26.325933 4 6.581483
-
Total (corrected) 78.187533 5
0 missing value(s) have been excluded
Table of means for THNHDO.ketqua by THNHDO.nt
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Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
27 3 72.233333 1.0898522 1.4811576 69.324430 75.142237 30 3 78.113333 1.7888202 1.4811576 75.204430 81.022237 -
Total 6 75.173333 1.0473366 1.0473366 73.116428 77.230239 Multiple range analysis for THNHDO.ketqua by THNHDO.nt
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Method: 95 Percent LSD
Level Count Average Homogeneous Groups
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27 3 72.233333 X
30 3 78.113333 X
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contrast difference limits
27 - 30 -5.88000 5.81781 *
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* denotes a statistically significant difference
Trang 3One-Way Analysis of Variance
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Data: THACHPH.ketqua
Level codes: THACHPH.nt
Labels:
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
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Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 1460.7687 4 365.19218 42.369 0000
Within groups 86.1927 10 8.61927
-
Total (corrected) 1546.9614 14
0 missing value(s) have been excluded
Table of means for THACHPH.ketqua by THACHPH.nt
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Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
5 3 74.376000 1.8895011 1.6950189 71.704731 77.047269 5.5 3 75.640000 1.8620150 1.6950189 72.968731 78.311269 6 3 70.100000 2.1383717 1.6950189 67.428731 72.771269 6.5 3 61.183333 1.1555999 1.6950189 58.512064 63.854602 7 3 49.151667 1.1916737 1.6950189 46.480398 51.822936 -
Total 15 66.090200 7580355 7580355 64.895572 67.284828 08/10/05 12:22:20 PM Page 1 Multiple range analysis for THACHPH.ketqua by THACHPH.nt
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Method: 95 Percent LSD
Level Count Average Homogeneous Groups
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7 3 49.151667 X
6.5 3 61.183333 X
6 3 70.100000 X
5 3 74.376000 XX
5.5 3 75.640000 X
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contrast difference limits
5 - 5.5 -1.26400 5.34254
5 - 6 4.27600 5.34254
5 - 6.5 13.1927 5.34254 *
5 - 7 25.2243 5.34254 *
5.5 - 6 5.54000 5.34254 *
5.5 - 6.5 14.4567 5.34254 *
5.5 - 7 26.4883 5.34254 *
6 - 6.5 8.91667 5.34254 *
6 - 7 20.9483 5.34254 *
6.5 - 7 12.0317 5.34254 *
-
* denotes a statistically significant difference
Trang 4
PHỤ LỤC 2: Kết quả phân tích thống kê các môi trường nhân giống cấp 2
One-Way Analysis of Variance
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Data: MTNGTG.ketqua
Level codes: MTNGTG.nt
Labels:
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
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Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 2236.2254 8 279.52818 35.786 0000
Within groups 140.5981 18 7.81100
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Total (corrected) 2376.8235 26
0 missing value(s) have been excluded
Table of means for MTNGTG.ketqua by MTNGTG.nt
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Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 92.94667 2.3487325 1.6135885 90.548971 95.34436 2 3 102.16667 2531359 1.6135885 99.768971 104.56436 3 3 94.91667 7936694 1.6135885 92.518971 97.31436 4 3 82.05667 1.7324966 1.6135885 79.658971 84.45436 5 3 100.91667 1.0999596 1.6135885 98.518971 103.31436 6 3 94.38667 9433333 1.6135885 91.988971 96.78436 7 3 77.89000 2.3120193 1.6135885 75.492305 80.28770 8 3 75.41667 2.1032382 1.6135885 73.018971 77.81436 9 3 92.14000 1.5336558 1.6135885 89.742305 94.53770 -
Total 27 90.31519 5378628 5378628 89.515953 91.11442 Multiple range analysis for MTNGTG.ketqua by MTNGTG.nt
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Method: 95 Percent LSD
Level Count Average Homogeneous Groups
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8 3 75.41667 X
7 3 77.89000 XX
4 3 82.05667 X
9 3 92.14000 X
1 3 92.94667 X
6 3 94.38667 X
3 3 94.91667 X
5 3 100.91667 X
2 3 102.16667 X
-
contrast difference limits
1 - 2 -9.22000 4.79539 *
1 - 3 -1.97000 4.79539
1 - 4 10.8900 4.79539 *
1 - 5 -7.97000 4.79539 *
1 - 6 -1.44000 4.79539
1 - 7 15.0567 4.79539 *
1 - 8 17.5300 4.79539 *
1 - 9 0.80667 4.79539
Trang 52 - 3 7.25000 4.79539 *
2 - 4 20.1100 4.79539 *
2 - 5 1.25000 4.79539
2 - 6 7.78000 4.79539 *
2 - 7 24.2767 4.79539 *
2 - 8 26.7500 4.79539 *
2 - 9 10.0267 4.79539 *
3 - 4 12.8600 4.79539 *
3 - 5 -6.00000 4.79539 *
3 - 6 0.53000 4.79539
3 - 7 17.0267 4.79539 *
3 - 8 19.5000 4.79539 *
3 - 9 2.77667 4.79539
4 - 5 -18.8600 4.79539 *
4 - 6 -12.3300 4.79539 *
4 - 7 4.16667 4.79539
4 - 8 6.64000 4.79539 *
4 - 9 -10.0833 4.79539 *
5 - 6 6.53000 4.79539 *
5 - 7 23.0267 4.79539 *
5 - 8 25.5000 4.79539 *
5 - 9 8.77667 4.79539 *
6 - 7 16.4967 4.79539 *
6 - 8 18.9700 4.79539 *
6 - 9 2.24667 4.79539
7 - 8 2.47333 4.79539
7 - 9 -14.2500 4.79539 *
8 - 9 -16.7233 4.79539 *
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* denotes a statistically significant difference
Trang 6PHỤ LỤC 3: Kết quả phân tích thống kê các thí nghiệm về khả năng tạo sinh khối nấm vân chi trong môi trường lỏng
One-Way Analysis of Variance
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Data: MTLTN1.ketqua
Level codes: MTLTN1.nt
Labels:
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
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Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 2255989 2 1127994 234.480 0000
Within groups .0028864 6 0004811
-
Total (corrected) .2284852 8
0 missing value(s) have been excluded
Table of means for MTLTN1.ketqua by MTLTN1.nt
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Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
ps 3 2101333 0024258 0126631 1882166 2320500
cra 3 0459333 0026934 0126631 0240166 0678500
bth 3 4323000 0216315 0126631 4103833 4542167
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Total 9 2294556 0073110 0073110 2168019 2421092
Multiple range analysis for MTLTN1.ketqua by MTLTN1.nt
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Method: 95 Percent LSD
Level Count Average Homogeneous Groups
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cra 3 0459333 X
ps 3 2101333 X
bth 3 4323000 X
-
contrast difference limits
ps - cra 0.16420 0.04383 *
ps - bth -0.22217 0.04383 *
cra - bth -0.38637 0.04383 *
-
* denotes a statistically significant difference
Trang 7
One-Way Analysis of Variance
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Data: MTL10MT.ketqua
Level codes: MTL10MT.nt
Labels:
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
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Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 1729553 9 0192173 25.185 0000
Within groups .0152606 20 0007630
-
Total (corrected) .1882159 29
0 missing value(s) have been excluded
Table of means for MTL10MT.ketqua by MTL10MT.nt
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Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 1581333 0371602 0159481 1346042 1816625
2 3 2276000 0108523 0159481 2040708 2511292
3 3 2432667 0090497 0159481 2197375 2667958
4 3 1973000 0100022 0159481 1737708 2208292
5 3 2047333 0104539 0159481 1812042 2282625
6 3 2074000 0076559 0159481 1838708 2309292
7 3 3402000 0090163 0159481 3166708 3637292
8 3 2332000 0094599 0159481 2096708 2567292
9 3 2289000 0074987 0159481 2053708 2524292
10 3 4323000 0216315 0159481 4087708 4558292
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Total 30 2473033 0050432 0050432 2398628 2547439
Multiple range analysis for MTL10MT.ketqua by MTL10MT.nt
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Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 1581333 X
4 3 1973000 XX
5 3 2047333 XX
6 3 2074000 X
2 3 2276000 X
9 3 2289000 X
8 3 2332000 X
3 3 2432667 X
7 3 3402000 X
10 3 4323000 X
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contrast difference +/- limits
1 - 2 -0.06947 0.04706 *
1 - 3 -0.08513 0.04706 *
1 - 4 -0.03917 0.04706
1 - 5 -0.04660 0.04706
1 - 6 -0.04927 0.04706 *
1 - 7 -0.18207 0.04706 *
1 - 8 -0.07507 0.04706 *
1 - 9 -0.07077 0.04706 *
Trang 81 - 10 -0.27417 0.04706 *
2 - 3 -0.01567 0.04706
2 - 4 0.03030 0.04706
2 - 5 0.02287 0.04706
2 - 6 0.02020 0.04706
2 - 7 -0.11260 0.04706 *
2 - 8 -0.00560 0.04706
2 - 9 -0.00130 0.04706
2 - 10 -0.20470 0.04706 *
3 - 4 0.04597 0.04706
3 - 5 0.03853 0.04706
3 - 6 0.03587 0.04706
3 - 7 -0.09693 0.04706 *
3 - 8 0.01007 0.04706
3 - 9 0.01437 0.04706
3 - 10 -0.18903 0.04706 *
4 - 5 -0.00743 0.04706
4 - 6 -0.01010 0.04706
4 - 7 -0.14290 0.04706 *
4 - 8 -0.03590 0.04706
4 - 9 -0.03160 0.04706
4 - 10 -0.23500 0.04706 *
5 - 6 -0.00267 0.04706
5 - 7 -0.13547 0.04706 *
5 - 8 -0.02847 0.04706
5 - 9 -0.02417 0.04706
5 - 10 -0.22757 0.04706 *
6 - 7 -0.13280 0.04706 *
6 - 8 -0.02580 0.04706
6 - 9 -0.02150 0.04706
6 - 10 -0.22490 0.04706 *
7 - 8 0.10700 0.04706 *
7 - 9 0.11130 0.04706 *
7 - 10 -0.09210 0.04706 *
8 - 9 0.00430 0.04706
8 - 10 -0.19910 0.04706 *
9 - 10 -0.20340 0.04706 *
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* denotes a statistically significant difference
Trang 9PHỤ LỤC 4: Kết quả phân tích thống kê các thí nghiệm ly trích hợp chất polysaccharide thô từ sinh khối nấm vân chi
One-Way Analysis of Variance
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Data: TLTN1.KETQUA
Level codes: TLTN1.NT
Labels:
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 240.89087 2 120.44543 1237.594 0000
Within groups .58393 6 .09732
-
Total (corrected) 241.47480 8
0 missing value(s) have been excluded
Table of means for TLTN1.KETQUA by TLTN1.NT
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Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
PSA 3 10.783333 1812304 1801131 10.471602 11.095065 BTH 3 11.156667 2539247 1801131 10.844935 11.468398 CRA 3 .000000 0000000 1801131 -.311731 311731
-
Total 9 7.313333 1039884 1039884 7.133355 7.493311 Multiple range analysis for TLTN1.KETQUA by TLTN1.NT
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Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
CRA 3 .000000 X
PSA 3 10.783333 X
BTH 3 11.156667 X
-
contrast difference limits
PSA - BTH -0.37333 0.62346
PSA - CRA 10.7833 0.62346 *
BTH - CRA 11.1567 0.62346 *
-
* denotes a statistically significant difference
Trang 10
One-Way Analysis of Variance
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Data: TL10MT.ketqua
Level codes: TL10MT.nt
Labels:
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 272.88421 9 30.320468 10.527 0000
Within groups 57.60673 20 2.880337
-
Total (corrected) 330.49095 29
0 missing value(s) have been excluded
Table of means for TL10MT.ketqua by TL10MT.nt
-
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 11.163333 3595058 9798532 9.717701 12.608966 2 3 11.650000 1985783 9798532 10.204368 13.095632 3 3 12.376667 2509537 9798532 10.931034 13.822299 4 3 11.676667 1.0785999 9798532 10.231034 13.122299 5 3 14.016667 8871365 9798532 12.571034 15.462299 6 3 14.546667 3919325 9798532 13.101034 15.992299 7 3 12.960000 1.0759802 9798532 11.514368 14.405632 8 3 21.753333 1.9380517 9798532 20.307701 23.198966 9 3 15.053333 1.5123528 9798532 13.607701 16.498966 10 3 11.156667 2539247 9798532 9.711034 12.602299 -
Total 30 13.635333 3098568 3098568 13.178184 14.092482 Multiple range analysis for TL10MT.ketqua by TL10MT.nt
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Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
10 3 11.156667 X
1 3 11.163333 X
2 3 11.650000 X
4 3 11.676667 XX
3 3 12.376667 XXX
7 3 12.960000 XXX
5 3 14.016667 XXX
6 3 14.546667 XX
9 3 15.053333 X
8 3 21.753333 X
-
contrast difference limits
1 - 2 -0.48667 2.89126
1 - 3 -1.21333 2.89126
1 - 4 -0.51333 2.89126
1 - 5 -2.85333 2.89126
1 - 6 -3.38333 2.89126 *
1 - 7 -1.79667 2.89126
1 - 8 -10.5900 2.89126 *
1 - 9 -3.89000 2.89126 *
1 - 10 0.00667 2.89126
2 - 3 -0.72667 2.89126
2 - 4 -0.02667 2.89126
