Ảnh hưởng của chất kích thích sinh trưởng 2,4-D và BAP đến khả năng hình thành mô sẹo từ PLB của cây lan Hồ Điệp in vitro Thời gian tạo mô sẹo Means plot: LSD Confidence level: 95 Ra
Trang 1PHỤ LỤC
THÍ NGHIỆM 1 Ảnh hưởng của chất kích thích sinh trưởng 2,4-D và BAP đến khả năng hình thành mô sẹo từ PLB của cây lan Hồ Điệp in vitro
Thời gian tạo mô sẹo
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 8.035523 12 6696269 .512 8878
Within groups 34.000000 26 1.3076923
-
Total (corrected) 42.035523 38
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 19.890000 5773503 6602253 18.930149 20.849851 2 3 20.890000 5773503 6602253 19.930149 21.849851 3 3 20.560000 1.0000000 6602253 19.600149 21.519851 4 3 20.560000 5773503 6602253 19.600149 21.519851 5 3 19.670000 0000000 6602253 18.710149 20.629851 6 3 19.780000 5773503 6602253 18.820149 20.739851 7 3 20.000000 0000000 6602253 19.040149 20.959851 8 3 20.220000 1.1547005 6602253 19.260149 21.179851 9 3 19.440000 5773503 6602253 18.480149 20.399851 10 3 20.440000 0000000 6602253 19.480149 21.399851 11 3 20.440000 1.1547005 6602253 19.480149 21.399851 12 3 21.000000 5773503 6602253 20.040149 21.959851 13 3 20.440000 0000000 6602253 19.480149 21.399851 -
Total 39 20.256154 1831135 1831135 19.989939 20.522369
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
9 3 19.440000 X
5 3 19.670000 X
6 3 19.780000 X
1 3 19.890000 X
7 3 20.000000 X
8 3 20.220000 X
10 3 20.440000 X
11 3 20.440000 X
13 3 20.440000 X
3 3 20.560000 X
4 3 20.560000 X
2 3 20.890000 X
12 3 21.000000 X
-
contrast difference limits
* denotes a statistically significant difference
Trang 2% mẫu cấy hình thành mô sẹo
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 9377.0647 12 781.42206 200.167 0000
Within groups 101.5000 26 3.90385
-
Total (corrected) 9478.5647 38
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 24.440000 0577350 1.1407375 22.781570 26.098430 2 3 46.670000 3464102 1.1407375 45.011570 48.328430 3 3 53.330000 1.1547005 1.1407375 51.671570 54.988430 4 3 68.890000 1.1547005 1.1407375 67.231570 70.548430 5 3 62.220000 1154701 1.1407375 60.561570 63.878430 6 3 48.890000 1.1547005 1.1407375 47.231570 50.548430 7 3 48.890000 5773503 1.1407375 47.231570 50.548430 8 3 80.000000 2.3094011 1.1407375 78.341570 81.658430 9 3 53.330000 1732051 1.1407375 51.671570 54.988430 10 3 42.220000 1.1547005 1.1407375 40.561570 43.878430 11 3 46.670000 2.3094011 1.1407375 45.011570 48.328430 12 3 35.560000 2886751 1.1407375 33.901570 37.218430 13 3 22.220000 5773503 1.1407375 20.561570 23.878430 -
Total 39 48.717692 3163837 3163837 48.257726 49.177658
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
13 3 22.220000 X
1 3 24.440000 X
12 3 35.560000 X
10 3 42.220000 X
11 3 46.670000 X
2 3 46.670000 X
6 3 48.890000 X
7 3 48.890000 X
3 3 53.330000 X
9 3 53.330000 X
5 3 62.220000 X
4 3 68.890000 X
8 3 80.000000 X
-
contrast difference limits
* denotes a statistically significant difference
Trang 3
% mẫu cấy hình thành mô sẹo + PLB
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 770.03003 12 64.169169 60.802 0000
Within groups 27.44000 26 1.055385
-
Total (corrected) 797.47003 38
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 15.550000 2886751 5931230 14.687704 16.412296 2 3 13.330000 1732051 5931230 12.467704 14.192296 3 3 15.560000 5773503 5931230 14.697704 16.422296 4 3 11.110000 1.1547005 5931230 10.247704 11.972296 5 3 15.560000 5773503 5931230 14.697704 16.422296 6 3 22.220000 5773503 5931230 21.357704 23.082296 7 3 13.330000 1.1547005 5931230 12.467704 14.192296 8 3 8.890000 1154701 5931230 8.027704 9.752296 9 3 6.670000 0000000 5931230 5.807704 7.532296 10 3 11.110000 5773503 5931230 10.247704 11.972296 11 3 20.000000 2886751 5931230 19.137704 20.862296 12 3 13.330000 1732051 5931230 12.467704 14.192296 13 3 6.670000 5773503 5931230 5.807704 7.532296 -
Total 39 13.333077 1645027 1645027 13.093919 13.572235
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
9 3 6.670000 X
13 3 6.670000 X
8 3 8.890000 X
4 3 11.110000 X
10 3 11.110000 X
2 3 13.330000 X
7 3 13.330000 X
12 3 13.330000 X
1 3 15.550000 X
3 3 15.560000 X
5 3 15.560000 X
11 3 20.000000 X
6 3 22.220000 X
-
contrast difference limits
* denotes a statistically significant difference
Trang 4
% mẫu cấy hình thành PLB
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 1988.7243 12 165.72703 30.454 0000
Within groups 141.4867 26 5.44179
-
Total (corrected) 2130.2110 38
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 33.330000 1.7320508 1.3468228 31.371958 35.288042 2 3 8.890000 1.7320508 1.3468228 6.931958 10.848042 3 3 6.670000 1.7320508 1.3468228 4.711958 8.628042 4 3 8.890000 5773503 1.3468228 6.931958 10.848042 5 3 15.893333 1.4529663 1.3468228 13.935291 17.851375 6 3 11.110000 1.1547005 1.3468228 9.151958 13.068042 7 3 4.440000 2309401 1.3468228 2.481958 6.398042 8 3 6.670000 5773503 1.3468228 4.711958 8.628042 9 3 8.890000 1.1547005 1.3468228 6.931958 10.848042 10 3 8.890000 1.7320508 1.3468228 6.931958 10.848042 11 3 13.330000 1.7320508 1.3468228 11.371958 15.288042 12 3 15.560000 2886751 1.3468228 13.601958 17.518042 13 3 6.670000 1.7320508 1.3468228 4.711958 8.628042 -
Total 39 11.479487 3735414 3735414 10.936424 12.022550
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
7 3 4.440000 X
3 3 6.670000 XX
8 3 6.670000 XX
13 3 6.670000 XX
2 3 8.890000 XX
4 3 8.890000 XX
9 3 8.890000 XX
10 3 8.890000 XX
6 3 11.110000 XX
11 3 13.330000 XX
12 3 15.560000 X
5 3 15.893333 X
1 3 33.330000 X
-
contrast difference limits
* denotes a statistically significant difference
Trang 5
% mẫu cấy không phản ứng + mẫu chết
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 8933.7662 12 744.48052 112.212 0000
Within groups 172.5000 26 6.63462
-
Total (corrected) 9106.2662 38
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 26.680000 2.3094011 1.4871242 24.517985 28.842015 2 3 31.110000 5773503 1.4871242 28.947985 33.272015 3 3 24.440000 2309401 1.4871242 22.277985 26.602015 4 3 11.110000 2.3094011 1.4871242 8.947985 13.272015 5 3 6.670000 5773503 1.4871242 4.507985 8.832015 6 3 17.780000 1.1547005 1.4871242 15.617985 19.942015 7 3 33.340000 1732051 1.4871242 31.177985 35.502015 8 3 4.440000 5773503 1.4871242 2.277985 6.602015 9 3 31.110000 2.8867513 1.4871242 28.947985 33.272015 10 3 37.780000 5773503 1.4871242 35.617985 39.942015 11 3 20.000000 1.1547005 1.4871242 17.837985 22.162015 12 3 35.550000 5773503 1.4871242 33.387985 37.712015 13 3 64.450000 2.3094011 1.4871242 62.287985 66.612015 -
Total 39 26.496923 4124540 4124540 25.897288 27.096558
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
8 3 4.440000 X
5 3 6.670000 X
4 3 11.110000 X
6 3 17.780000 X
11 3 20.000000 X
3 3 24.440000 X
1 3 26.680000 X
2 3 31.110000 X
9 3 31.110000 X
7 3 33.340000 XX
12 3 35.550000 XX
10 3 37.780000 X
13 3 64.450000 X
-
contrast difference limits
* denotes a statistically significant difference
Trang 6
Thí nghiệm 2: Ảnh hưởng của nồng độ đường và nước dừa đến khả năng hình
thành mô sẹo từ PLB của cây lan Hồ Điệp in vitro
Thời gian hình thành mô sẹo
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 1128.1788 8 141.02235 22.251 0000
Within groups 114.0800 18 6.33778
-
Total (corrected) 1242.2588 26
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 .000000 0000000 1.4534760 -2.159778 2.159778 2 3 21.110000 5773503 1.4534760 18.950222 23.269778 3 3 20.890000 1.1547005 1.4534760 18.730222 23.049778 4 3 19.890000 1.7320508 1.4534760 17.730222 22.049778 5 3 21.110000 5773503 1.4534760 18.950222 23.269778 6 3 20.220000 1154701 1.4534760 18.060222 22.379778 7 3 20.220000 2.8867513 1.4534760 18.060222 22.379778 8 3 20.560000 2.3094011 1.4534760 18.400222 22.719778 9 3 20.220000 5773503 1.4534760 18.060222 22.379778 -
Total 27 18.246667 4844920 4844920 17.526741 18.966593
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 .000000 X
4 3 19.890000 X
6 3 20.220000 X
7 3 20.220000 X
9 3 20.220000 X
8 3 20.560000 X
3 3 20.890000 X
2 3 21.110000 X
5 3 21.110000 X
-
contrast difference limits
1 - 2 -21.1100 4.31956 *
1 - 3 -20.8900 4.31956 *
1 - 4 -19.8900 4.31956 *
1 - 5 -21.1100 4.31956 *
* denotes a statistically significant difference
Trang 7
% mẫu cấy hình thành mô sẹo
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 12043.132 8 1505.3915 205.156 0000
Within groups 132.080 18 7.3378
-
Total (corrected) 12175.212 26
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 .000000 0000000 1.5639456 -2.323929 2.323929 2 3 60.000000 2.3094011 1.5639456 59.676071 64.323929 3 3 55.560000 1.7320508 1.5639456 53.236071 57.883929 4 3 48.890000 1.1547005 1.5639456 46.566071 51.213929 5 3 64.440000 1.1547005 1.5639456 62.116071 66.763929 6 3 55.560000 2.8867513 1.5639456 53.236071 57.883929 7 3 80.000000 1.1547005 1.5639456 77.676071 82.323929 8 3 62.220000 1154701 1.5639456 59.896071 64.543929 9 3 66.670000 1.1547005 1.5639456 64.346071 68.993929 -
Total 27 55.037778 5213152 5213152 54.263135 55.812421
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 .000000 X
4 3 48.890000 X
3 3 55.560000 X
6 3 55.560000 X
2 3 60.000000 X
8 3 62.220000 XX
5 3 64.440000 XX
9 3 66.670000 X
7 3 80.000000 X
-
contrast difference limits
1 - 2 -62.0000 4.64786 *
1 - 3 -55.5600 4.64786 *
1 - 4 -48.8900 4.64786 *
1 - 5 -64.4400 4.64786 *
* denotes a statistically significant difference
Trang 8
% mẫu cấy hình thành mô sẹo+PLB
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 704.85120 8 88.106400 65.860 0000
Within groups 24.08000 18 1.337778
-
Total (corrected) 728.93120 26
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 .000000 0000000 6677769 -.992276 992276
2 3 15.560000 1.1547005 6677769 14.567724 16.552276 3 3 17.780000 5773503 6677769 16.787724 18.772276 4 3 11.110000 1.1547005 6677769 10.117724 12.102276 5 3 8.890000 1154701 6677769 7.897724 9.882276 6 3 11.110000 5773503 6677769 10.117724 12.102276 7 3 8.890000 0000000 6677769 7.897724 9.882276 8 3 6.670000 5773503 6677769 5.677724 7.662276 9 3 4.440000 5773503 6677769 3.447724 5.432276 -
Total 27 9.383333 2225923 2225923 9.052575 9.714092
Multiple range analysis
-
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 .000000 X
9 3 4.440000 X
8 3 6.670000 X
5 3 8.890000 X
7 3 8.890000 X
4 3 11.110000 X
6 3 11.110000 X
2 3 15.560000 X
3 3 17.780000 X
-
contrast difference limits
1 - 2 -15.5600 1.98455 *
1 - 3 -17.7800 1.98455 *
1 - 4 -11.1100 1.98455 *
1 - 5 -8.89000 1.98455 *
* denotes a statistically significant difference
Trang 9
% mẫu cấy hình thành PLB
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 9760.4610 8 1220.0576 645.533 0000
Within groups 34.0200 18 1.8900
-
Total (corrected) 9794.4810 26
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 71.110000 1.1547005 7937254 69.930572 72.289428 2 3 11.110000 5773503 7937254 9.930572 12.289428 3 3 8.890000 0577350 7937254 7.710572 10.069428 4 3 22.220000 5773503 7937254 21.040572 23.399428 5 3 8.890000 1.1547005 7937254 7.710572 10.069428 6 3 8.890000 5773503 7937254 7.710572 10.069428 7 3 6.670000 5773503 7937254 5.490572 7.849428 8 3 22.220000 1.1547005 7937254 21.040572 23.399428 9 3 22.220000 5773503 7937254 21.040572 23.399428 -
Total 27 20.246667 2645751 2645751 19.853524 20.639809
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
7 3 6.670000 X
3 3 8.890000 XX
5 3 8.890000 XX
6 3 8.890000 XX
2 3 11.110000 X
4 3 22.220000 X
8 3 22.220000 X
9 3 22.220000 X
1 3 71.110000 X
-
contrast difference limits
1 - 2 60.0000 2.35886 *
1 - 3 62.2200 2.35886 *
1 - 4 48.8900 2.35886 *
1 - 5 62.2200 2.35886 *
* denotes a statistically significant difference
Trang 10
% mẫu cấy không phản ứng + mẫu chết
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 1570.9632 8 196.37040 47.714 0000
Within groups 74.0800 18 4.11556
-
Total (corrected) 1645.0432 26
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 28.890000 1.1547005 1.1712608 27.149577 30.630423 2 3 13.330000 1.1547005 1.1712608 11.589577 15.070423 3 3 17.780000 1154701 1.1712608 16.039577 19.520423 4 3 17.780000 0000000 1.1712608 16.039577 19.520423 5 3 17.780000 1.1547005 1.1712608 16.039577 19.520423 6 3 24.440000 2.3094011 1.1712608 22.699577 26.180423 7 3 4.440000 1.1547005 1.1712608 2.699577 6.180423 8 3 28.890000 5773503 1.1712608 27.149577 30.630423 9 3 26.670000 1.1547005 1.1712608 24.929577 28.410423 -
Total 27 20.000000 3904203 3904203 19.419859 20.580141
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
7 3 4.440000 X
2 3 13.330000 X
3 3 17.780000 X
4 3 17.780000 X
5 3 17.780000 X
6 3 24.440000 X
9 3 26.670000 XX
1 3 28.890000 X
8 3 28.890000 X
-
contrast difference limits
1 - 2 15.5600 3.48085 *
1 - 3 11.1100 3.48085 *
1 - 4 11.1100 3.48085 *
1 - 5 11.1100 3.48085 *
* denotes a statistically significant difference