Thí nghiệm 3 : Ảnh hưởng của chất kích thích sinh trưởng TDZ trong điều kiện môi trường đặc, lỏng lắc đến khả năng hình thành phôi soma từ mô sẹo của cây lan Hồ Điệp in vitro S ố phôi
Trang 1Thí nghiệm 3 : Ảnh hưởng của chất kích thích sinh trưởng TDZ trong điều
kiện môi trường đặc, lỏng lắc đến khả năng hình thành phôi soma từ mô sẹo của
cây lan Hồ Điệp in vitro
S ố phôi tạo ra từ môi trường đặc
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 188.77344 4 47.193360 38.747 0000
Within groups 12.18000 10 1.218000
-
Total (corrected) 200.95344 14
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 .000000 0000000 6371813 -1.0041673 1.004167 2 3 2.670000 5773503 6371813 1.6658327 3.674167 3 3 4.330000 1732051 6371813 3.3258327 5.334167 4 3 10.670000 5773503 6371813 9.6658327 11.674167 5 3 5.670000 1.1547005 6371813 4.6658327 6.674167 -
Total 15 4.668000 2849561 2849561 4.2189227 5.117077
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 .000000 X
2 3 2.670000 X
3 3 4.330000 XX
5 3 5.670000 X
4 3 10.670000 X
-
contrast difference limits
1 - 2 -2.67000 2.00833 *
1 - 3 -4.33000 2.00833 *
1 - 4 -10.6700 2.00833 *
1 - 5 -5.67000 2.00833 *
2 - 3 -1.66000 2.00833
2 - 4 -8.00000 2.00833 *
2 - 5 -3.00000 2.00833 *
3 - 4 -6.34000 2.00833 *
* denotes a statistically significant difference
Trang 2
S ố phôi tạo ra từ môi trường lỏng lắc
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 2931.9868 4 732.99669 261.039 0000
Within groups 28.0800 10 2.80800
-
Total (corrected) 2960.0668 14
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 .000000 0000000 9674709 -1.524688 1.524688 2 3 4.330000 5773503 9674709 2.805312 5.854688 3 3 12.670000 1154701 9674709 11.145312 14.194688 4 3 39.670000 1.1547005 9674709 38.145312 41.194688 5 3 20.000000 1.7320508 9674709 18.475312 21.524688 -
Total 15 15.334000 4326662 4326662 14.652139 16.015861
Multiple range analysis
-
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 .000000 X
2 3 4.330000 X
3 3 12.670000 X
5 3 20.000000 X
4 3 39.670000 X
-
contrast difference limits
1 - 2 -4.33000 3.04938 *
1 - 3 -12.6700 3.04938 *
1 - 4 -39.6700 3.04938 *
1 - 5 -20.0000 3.04938 *
2 - 3 -8.34000 3.04938 *
2 - 4 -35.3400 3.04938 *
2 - 5 -15.6700 3.04938 *
3 - 4 -27.0000 3.04938 *
* denotes a statistically significant difference
Trang 3
Thí nghiệm 4 : Ảnh hưởng của các loại môi trường đến quá trình tái sinh cây lan Hồ Điệp in vitro từ phôi soma Tỉ l ệ mẫu chết Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 2481.8963 7 354.55662 47.817 0000
Within groups 118.6371 16 7.41482
-
Total (corrected) 2600.5334 23
0 missing value(s) have been excluded
Table of means
-
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 26.670000 0000000 1.5721341 24.312787 29.027213 2 3 24.446667 2.2233333 1.5721341 22.089454 26.803879 3 3 4.446667 2.2233333 1.5721341 2.089454 6.803879 4 3 2.223333 2.2233333 1.5721341 -.133879 4.580546 5 3 6.670000 0000000 1.5721341 4.312787 9.027213 6 3 2.223333 2.2233333 1.5721341 -.133879 4.580546 7 3 .000000 0000000 1.5721341 -2.357213 2.357213 8 3 .000000 0000000 1.5721341 -2.357213 2.357213 -
Total 24 8.335000 5558333 5558333 7.501599 9.168401
Multiple range analysis
-
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
7 3 .000000 X
8 3 .000000 X
4 3 2.223333 XX
6 3 2.223333 XX
3 3 4.446667 XX
5 3 6.670000 X
2 3 24.446667 X
1 3 26.670000 X
-
contrast difference limits
1 - 2 2.22333 4.71443
1 - 3 22.2233 4.71443 *
1 - 4 24.4467 4.71443 *
1 - 5 20.0000 4.71443 *
1 - 6 24.4467 4.71443 *
* denotes a statistically significant difference
Trang 4
H ệ số nhân chồi
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 381.55196 7 54.507423 78.569 0000
Within groups 11.10000 16 .693750
-
Total (corrected) 392.65196 23
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 .000000 0000000 4808846 -.721025 721025
2 3 .780000 0577350 4808846 058975 1.501025 3 3 1.440000 2309401 4808846 718975 2.161025 4 3 9.000000 1.1547005 4808846 8.278975 9.721025 5 3 .440000 1154701 4808846 -.281025 1.161025 6 3 3.000000 2886751 4808846 2.278975 3.721025 7 3 11.340000 1732051 4808846 10.618975 12.061025 8 3 5.330000 5773503 4808846 4.608975 6.051025 -
Total 24 3.916250 1700184 1700184 3.661329 4.171171
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 .000000 X
5 3 .440000 X
2 3 .780000 X
3 3 1.440000 X
6 3 3.000000 X
8 3 5.330000 X
4 3 9.000000 X
7 3 11.340000 X
-
contrast difference limits
1 - 2 -0.78000 1.44205
1 - 3 -1.44000 1.44205
1 - 4 -9.00000 1.44205 *
1 - 5 -0.44000 1.44205
1 - 6 -3.00000 1.44205 *
* denotes a statistically significant difference
Trang 5
S ố lá/chồi
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 3.9647625 7 5663946 462.363 0000
Within groups .0196000 16 0012250
-
Total (corrected) 3.9843625 23
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 0000000 0000000 0202073 -.0302982 0302982
2 3 1.0000000 0000000 0202073 9697018 1.0302982 3 3 1.0000000 0000000 0202073 9697018 1.0302982 4 3 1.2300000 0173205 0202073 1.1997018 1.2602982 5 3 1.0000000 0000000 0202073 9697018 1.0302982 6 3 1.2300000 0346410 0202073 1.1997018 1.2602982 7 3 1.3800000 0115470 0202073 1.3497018 1.4102982 8 3 1.2700000 0404145 0202073 1.2397018 1.3002982 -
Total 24 1.0137500 0071443 0071443 1.0030380 1.0244620
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 0000000 X
2 3 1.0000000 X
3 3 1.0000000 X
5 3 1.0000000 X
6 3 1.2300000 X
4 3 1.2300000 X
8 3 1.2700000 X
7 3 1.3800000 X
-
contrast difference limits
1 - 2 -1.00000 0.06060 *
1 - 3 -1.00000 0.06060 *
1 - 4 -1.23000 0.06060 *
1 - 5 -1.00000 0.06060 *
1 - 6 -1.23000 0.06060 *
* denotes a statistically significant difference
Trang 6
S ố rễ/chồi
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 2386500 7 0340929 136.371 0000
Within groups .0040000 16 0002500
-
Total (corrected) .2426500 23
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 0000000 0000000 0091287 -.0136873 0136873
2 3 0000000 0000000 0091287 -.0136873 0136873
3 3 0000000 0000000 0091287 -.0136873 0136873
4 3 0000000 0000000 0091287 -.0136873 0136873
5 3 0000000 0000000 0091287 -.0136873 0136873
6 3 2400000 0230940 0091287 2263127 2536873
7 3 2200000 0115470 0091287 2063127 2336873
8 3 0000000 0000000 0091287 -.0136873 0136873
-
Total 24 0575000 0032275 0032275 0526608 0623392
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 0000000 X
2 3 0000000 X
3 3 0000000 X
4 3 0000000 X
5 3 0000000 X
8 3 0000000 X
7 3 2200000 X
6 3 2400000 X
-
contrast difference limits
1 - 2 0.00000 0.02737
1 - 3 0.00000 0.02737
1 - 4 0.00000 0.02737
1 - 5 0.00000 0.02737
1 - 6 -0.24000 0.02737 *
* denotes a statistically significant difference
Trang 7Chi ều dài rễ
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 66.345863 7 9.4779804 60.659 0000
Within groups 2.500000 16 1562500
-
Total (corrected) 68.845863 23
0 missing value(s) have been excluded
Table of means -
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 0000000 0000000 2282177 -.3421831 3421831
2 3 0000000 0000000 2282177 -.3421831 3421831
3 3 0000000 0000000 2282177 -.3421831 3421831
4 3 0000000 0000000 2282177 -.3421831 3421831
5 3 0000000 0000000 2282177 -.3421831 3421831
6 3 3.6700000 5773503 2282177 3.3278169 4.0121831 7 3 4.0000000 2886751 2282177 3.6578169 4.3421831 8 3 0000000 0000000 2282177 -.3421831 3421831
-
Total 24 9587500 0806872 0806872 8377700 1.0797300
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
1 3 0000000 X
2 3 0000000 X
3 3 0000000 X
4 3 0000000 X
5 3 0000000 X
8 3 0000000 X
6 3 3.6700000 X
7 3 4.0000000 X
-
contrast difference limits
1 - 2 0.00000 0.68437
1 - 3 0.00000 0.68437
1 - 4 0.00000 0.68437
1 - 5 0.00000 0.68437
1 - 6 -3.67000 0.68437 *
* denotes a statistically significant difference
Trang 8
Thí nghiệm 5 : Ảnh hưởng của môi trường tạo vỏ hạt đến sự tái sinh cây con từ hạt
nhân tạo
Means plot: LSD Confidence level: 95 Range test: LSD
Analysis of variance
-
Source of variation Sum of Squares d.f Mean square F-ratio Sig level -
Between groups 4434.6323 5 886.92645 126.704 0000
Within groups 84.0000 12 7.00000
-
Total (corrected) 4518.6323 17
0 missing value(s) have been excluded
Table of means
-
Stnd Error Stnd Error 95 % LSD
Level Count Average (internal) (pooled s) intervals for mean -
1 3 44.440000 1.1547005 1.5275252 42.086005 46.793995 2 3 77.780000 1.1547005 1.5275252 75.426005 80.133995 3 3 37.780000 1.1547005 1.5275252 35.426005 40.133995 4 3 55.560000 5773503 1.5275252 53.206005 57.913995 5 3 71.110000 2.8867513 1.5275252 68.756005 73.463995 6 3 37.780000 1.1547005 1.5275252 35.426005 40.133995 -
Total 18 54.075000 6236096 6236096 53.113986 55.036014
Multiple range analysis -
Method: 95 Percent LSD
Level Count Average Homogeneous Groups
-
3 3 37.780000 X
6 3 37.780000 X
1 3 44.440000 X
4 3 55.560000 X
5 3 71.110000 X
2 3 77.780000 X
-
contrast difference limits
1 - 2 -33.3400 4.70799 *
1 - 3 6.66000 4.70799 *
1 - 4 -11.1200 4.70799 *
1 - 5 -26.6700 4.70799 *
1 - 6 6.66000 4.70799 *
2 - 3 40.0000 4.70799 *
2 - 4 22.2200 4.70799 *
* denotes a statistically significant difference