In addition, increased concentrations of the ubiquitous small heat shock protein aB-crystallin were also detected by the proteomic profiling of senescent fibres Doran et al.. The age-dep
Trang 1The chaperone cvHsp appears to counter-act deleterious protein aggregation in the cytosol, sarcolemma and actomyosin apparatus of aged muscle (Doran et al
2007c ) In addition, increased concentrations of the ubiquitous small heat shock protein aB-crystallin were also detected by the proteomic profiling of senescent fibres (Doran et al 2008 ) The family of small heat shock proteins quickly responds during stressful conditions and facilitates the disintegration of poly-disperse assemblies into smaller subunits This process is ATP-independent whereby small chaperone subunits bind to unfolding substrate and then reform into larger complexes (Stamler et al 2005 ) The age-dependent activation of the cytoprotective protein complement of skeletal muscles seems to counter-act increased levels of denatured proteins in senescent fibres, especially abundant elements such as non-functional myosins, actins, troponins and tropomysoins (Vandervoort 2002 ; Prochniewicz et al 2007 ) Increased chaperone levels repre-sent an esrepre-sential cellular rescue mechanism for eliminating the potentially destructive accumulation of inactive muscle protein aggregates During aging, adaptive fibre transformation occurs in skeletal muscles The fast-to-slow transi-tion process encompasses major cellular remodeling This includes the degenera-tion of the fastest-twitching fibre populadegenera-tion, the activadegenera-tion of the satellite pool of muscle precursor cells and a certain degree of phenotypic fibre shifting within a contractile unit However, since senescent muscles have a reduced regenerative capacity, adaptive fibre modulation probably triggers excessive detrimental pro-tein aggregation as compared to healthy adult tissues This in turn requires a massive cellular stress response to prevent contractile dysfunction Therefore, in the context of a blunted stress response involving large heat shock proteins in aged muscle (Kayani et al 2008 ), the drastic up-regulation of low-molecular-mass chaperones probably represents a compensatory mechanism that mostly supports filament remodeling (Doran et al 2007c ).
Continuous contractile activity clearly influences the expression of heat shock proteins (Neufer et al 1998 ) Key chaperones containing the a-crystallin domain are up-regulated following chronic contraction patterns (Donoghue et al 2007 ) In analogy to chronic neuromuscular activity, similar fibre transition processes occur
in aged muscle The concomitant damage to the actomyosin apparatus and associ-ated cytoskeletal network may therefore trigger an increased synthesis of small heat shock proteins (Doran et al 2009a ) In contrast, cellular stress does not generate a sufficient response by larger heat shock proteins, such as those encoded by the Hsp70 gene (Liu et al 2006 ) The up-regulation of Hsp70 and related chaperones
is usually part of a highly coordinated stress response that prevents extensive mus-cular atrophy by limiting the stress-induced rate of cellular degeneration (Chung and Ng 2006 ) High levels of Hsp70 are essential for the stabilization of metabolic pathways, the prevention of high rates of apoptosis and the facilitation of physio-logical adaptation to changed functional demands An age-related impairment of the Hsp70 response is believed to play a key role in contractile deficits (McArdle
et al 2004 ) It is therefore not surprising that skeletal muscles of aged transgenic mice with over-expressed levels of Hsp70 are partially protected against fibre degeneration (Broome et al 2006 ).
Trang 2This suggests that a well-designed pharmacological approach to enhance the natural stress response could potentially eliminate excessive fibre damage in aged muscle In other areas of biomedicine, the drug-induced modulation of the cellular stress response has already gained considerable attention, as reviewed by Soti et al ( 2005 ) Various inducers, co-inducers and inhibitors of specific heat shock proteins are currently evaluated as emerging therapeutic vehicles for the treatment of heart disease, diabetes, cancer and neurodegenerative disorders (Calderwood et al 2006 ; Shamaei-Tousi et al 2007 ) Since the up-regulation of small heat shock proteins, such as aB-crystallin or cvHsp, may represent an auto-protective mechanism in senescent muscle, a further increase in their expression levels may have therapeutic benefits Hence, a pharmacologically mediated increase in essential muscle chap-erones may be a realistic treatment option for eliminating certain neuromuscular impairments and could decisively improve the survival rate of stressed motor units
in the senescent body.
3.4 Excitation–Contraction Uncoupling in Aged Muscle
Ca2+-fluxes represent one of the most crucial second messenger system in contrac-tile tissues (Berchtold et al 2000 ) Alterations in Ca2+-levels do not only affect protein activity and key physiological processes, but also gene expression patterns
in skeletal muscle Changes in the cytosolic Ca2+-concentration play a key role in myogenesis, differentiation, fibre transformation, metabolic regulation, excitation– contraction coupling and muscle relaxation Importantly, cyclic alterations in cyto-solic Ca2+-levels determine the contractile status of skeletal muscle fibres The regulation of Ca2+-homoeostasis and the mediation of the excitation–contraction– relaxation cycle depend on a finely tuned interplay between voltage-sensing recep-tors in the transverse tubules, Ca2+-release channel units in the junctional sarcoplasmic reticulum, luminal and cytosolic Ca2+-binding proteins, and Ca2+ -pumps of the sarcoplasmic reticulum, as well as minor structural components of the triad junction and sarcolemmal ion-regulatory elements such as ion exchangers and ion pumps (Murray et al 1998 ) It is therefore not surprising that abnormal Ca2+ -handling is involved in a variety of muscle pathologies (MacLennan 2000 ; Froemming and Ohlendieck 2001 ), including sarcopenia of old age (Renganathan
et al 1997 ; O’Connell et al 2008 b).
The physical coupling between the voltage-sensing a1S-subunit of the trans-verse-tubular dihydropyridine receptor and the ryanodine receptor Ca2+-release channel of the junctional sarcoplasmic reticulum forms the central signal transduc-tion unit during excitatransduc-tion–contractransduc-tion coupling in mature skeletal muscles (MacLennan et al 2002 ) The dihydropyridine receptor from skeletal muscle con-sists of a a1S-a2/d-b-g configuration The a1S-subunit represents the principal ion channel pore with three cytoplasmic loops between four repeat segments, whereby the II-III loop domain interacts directly with the junctional calcium release channel During muscle aging, a drastically lowered supply of Ca2+-ions to contractile
Trang 3proteins occurs due to uncoupling between the two main triad receptors (Renganathan et al 1997 ) Excitation–contraction uncoupling appears to be due to
a larger number of ryanodine receptors being uncoupled to the voltage-sensing dihydropyridine receptor units as compared to mature fibres A pathophysiological disconnection between sarcolemmal excitation and muscle contraction may result
in alterations in the voltage-gated Ca2+-release mechanism, decreases in myoplas-mic Ca2+-elevation in response to surface depolarisation, reduced Ca2+-supply to the actomyosin apparatus and reduced contractile strength Thus, abnormal Ca2+ -handling may account for a significant proportion of the decay in skeletal muscle force during aging (Delbono et al 1995 ) A recent immunoblotting and immuno-fluorescence survey has confirmed the excitation–contraction coupling hypothesis The Ca2+-binding protein named sarcalumenin, which represents a major mediator
of ion shuttling within the longitudinal sarcoplasmic reticulum, was shown to be greatly reduced in aged rat gastrocnemius muscle as compared to young adult specimens (O’Connell et al 2008 b) In addition, key elements of the plasmalemma-associated Ca2+-extrusion system, i.e the calmodulin-dependent Ca2+-ATPase and the Na+-Ca2+-exchanger, were also found to be diminished in aged muscle.
Figure 4 summarizes the findings of the immunoblotting survey of essential physi-ological regulators of Ca2+-homeostasis and how their dysregulation may affect the excitation–contraction–relaxation cycle during aging The overall protein band pat-tern of electrophoretically separated crude tissue extracts from 3-month versus 30-month old rat gastrocnemius muscle was very comparable between young adult versus senescent fibres The previously reported senescence-related decrease in the
a1S-subunit of the dihydropryridine receptor, but not its auxiliary a2-subunit, was confirmed Immunoblotting of the sarcoplasmic reticulum proteins that mediate Ca2+ -buffering and Ca2+-removal, i.e fast and slow calsequestrins and the Ca2+-pumping ATPase isoforms SERCA1 and SERCA2, suggested a shift to a slower phenotype, but these findings are not statistically significant In contrast, the reduced expression of the 160 kDa Ca2+-binding protein sarcalumenin and its related glycoprotein product
of 53 kDa, as well as the Na+-Ca2+-exchanger and the PMCA-type Ca2+-ATPase was shown to be significant in aged muscle Thus, downstream from the coupling defect between the dihydropyridine receptor and the junctional Ca2+-release channel, addi-tional age-dependent changes appear to exist in Ca2+-regulatory elements Reduced levels of sarcalumenin and the two sarcolemmal Ca2+-extrusion proteins may cause abnormal luminal Ca2+-binding and impaired Ca2+-removal (O’Connell et al 2008 b) This in turn could exacerbate disturbed ion fluxes and diminished triad signaling in senescent muscle and thereby contribute to contractile weakness.
4 Conclusion
Natural aging is a fundamental biological process The functional decline of skeletal muscle fibres and the loss of total muscle mass are crucial factors that render the human body more susceptible to a metabolic disequilibrium and physical
Trang 4weakness Besides studying the histological and anatomical effects of muscle aging
on frailty and fragility, it is also crucial to determine the molecular mechanisms that underlie age-dependent alterations at the cellular level The application of modern proteomic methodology for analysing age-related impairments in contractile tissues promises to elucidate the pathobiochemical processes that lead to sarcopenia of old age Mass spectrometry represents an unrivalled technique for the swift and reliable identification of protein factors involved in pathological pathways or compensatory
Fig 4 Overview of the excitation–contraction uncoupling hypothesis of skeletal muscle aging and comparative immunoblot analysis of key Ca2+-handling proteins in young adult versus senescent muscle Shown is a Coomassie-stained gel and immunoblots of young adult versus aged rat gastrocnemius preparations Immunoblots were labeled with antibodies to key proteins of the sarcolemma (SL), transverse tubules (TT) and sarcoplasmic reticulum (SR), including sarcalumenin (SAR) and its alternative splice product, the 53 kDa sarcoplasmic reticulum glycoprotein (53-SRGP), fast and slow calsequsetrin (fast CSQf; slow CSQs), fast and slow sarcoplasmic reticulum
Ca2+-ATPase (fast SERCA1; slow SERCA2), the Na+-Ca2+-exchanger (NCX), the plasmalemmal
Ca2+-ATPase (PMCA), and the a1S- and a2-subunit of the dihydropryridine receptor (DHPR) Molecular mass standards (in kDa) are indicated on the left of the Coomassie-stained gel panel
The comparative blotting was statistically evaluated using an unpaired Student’s t-test (n = 6;
*p < 0.05; **p < 0.01 Standard methods were employed for muscle preparations from crude tissue extracts, one-dimensional gel electrophoresis and immunoblot analysis of Ca2+-handling proteins (O’Connell et al 2007) The central panel outlines the dysregulation of Ca2+-fluxes in senescent fibres and how this may affect the excitation–contraction–relaxation cycle during skeletal muscle aging Besides the Ca2+-handling proteins that have been analysed by immunoblotting, other key elements of ion homeostasis and muscle regulation are included in this diagram, i.e the ryanodine receptor (RyR) Ca2+-release channel of the sarcoplasmic reticulum and the troponin subunit TnC
Trang 5mechanisms involved in aging Over the last few years, mass spectrometry-based proteomics has identified a large number of relatively sarcopenia-specific biomark-ers Skeletal muscle proteins that exhibit altered expression levels or changed post-translational modifications during aging include regulatory proteins, contractile elements, metabolic enzymes and cellular stress proteins The complexity of the observed changes in the senescent muscle proteome confirm the idea that sarcope-nia is probably based on a multi-factorial etiology, rather than alterations in just one class of protein factors, regulatory mechanisms or aging-inducing gene clusters Proteomic profiling studies have established distinct switches in fibre type-specific isoforms of contractile and metabolic proteins during aging, demonstrating an age-related transformation to slower-twitching muscles The fast-to-slow transition process is accompanied by bioenergetic adaptation mechanisms The comparative proteomic analysis of adult versus senescent muscles has clearly revealed a drastic shift to more aerobic-oxidative metabolism during aging The proteomic identifica-tion of new sarcopenic biomarkers and their detailed cell biological, physiological and biochemical characterzation will hopefully lead to the prompt development of superior diagnostic tools and the improved design of pharmacological strategies to counter-act the age-induced loss of contractile tissue Since alterations in the neu-romuscular system are of central importance for comprehending the overall patho-genesis of the aging process in humans, the recent findings from proteomic studies will be crucial for improving our general biomedical knowledge on the mechanisms
of aging.
Acknowledgements Research in the author’s laboratory was supported by a principal investiga-tor grant from Science Foundation Ireland (SFI-04/IN3/B614) and equipment grants from the Irish Health Research Board (HRB-EQ/2003/3, HRB-EQ/2004/2) and the Higher Education Authority (HEA-RERGS-07-NUIM) The authors thank Dr Marina Lynch (Trinity College Dublin) for her generous help obtaining aged rat muscle, and Ms Caroline Batchlor (NUI Maynooth) for assis-tance with mass spectrometry
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