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Chapter 101. Hemolytic Anemias and Anemia Due to Acute Blood Loss (Part 12) doc

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A very small minority of subjects with G6PD deficiency have CNSHA of variable severity.. Although hemolysis is, by definition, chronic in these patients, they are also vulnerable to acut

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Chapter 101 Hemolytic Anemias and Anemia

Due to Acute Blood Loss

(Part 12)

Figure 101-6

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Peripheral blood smear from a 5-year-old G6PD-deficient boy with acute

favism

A very small minority of subjects with G6PD deficiency have CNSHA of variable severity The patient is always a male, usually with a history of NNJ, who may present with anemia or unexplained jaundice, or because of gallstones later in life The spleen may be enlarged The severity of anemia ranges from borderline to transfusion-dependent The anemia is usually normo-macrocytic, with reticulocytosis Bilirubin and LDH are increased Although hemolysis is, by definition, chronic in these patients, they are also vulnerable to acute oxidative damage, and therefore the same agents (see Table 101-5) that can cause acute HA

in people with the ordinary type of G6PD deficiency will cause severe exacerbations in people with the severe form of G6PD deficiency In some cases

of CNSHA, the deficiency of G6PD is so severe in granulocytes that it becomes rate-limiting for their oxidative burst, with consequent increased susceptibility to bacterial infections

Laboratory Diagnosis

The suspicion of G6PD deficiency can be confirmed by semiquantitative methods often referred to as screening tests, which are suitable for population

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studies and can correctly classify male subjects, in the steady state, as G6PD-normal or G6PD-deficient However, in clinical practice a diagnostic test is usually needed when the patient has had a hemolytic attack: this implies that the oldest, most G6PD-deficient red cells have been selectively destroyed, and young red cells, having higher G6PD activity, are being released into the circulation Under these conditions, only a quantitative test can give a definitive result In males this test will identify normal hemizygotes and G6PD-deficient hemizygotes; among females some heterozygotes will be missed, but those who are at most risk

of hemolysis will be identified

G6PD Deficiency: Treatment

The acute HA of G6PD deficiency is largely preventable by avoiding exposure to triggering factors of previously screened subjects Of course, the practicability and cost-effectiveness of screening depends on the prevalence of G6PD deficiency in each individual community Favism is entirely preventable by not eating fava beans Prevention of drug-induced hemolysis is possible in most cases by choosing alternative drugs When acute HA develops and once its cause

is recognized, no specific treatment is needed in most cases However, if the anemia is severe, it may be a medical emergency, especially in children, requiring immediate action, including blood transfusion If acute renal failure develops,

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hemodialysis may be necessary, but if there is no previous kidney disease, full recovery is the rule The management of NNJ associated with G6PD deficiency is

no different from that of NNJ due to other causes

In cases with CNSHA, if the anemia is not severe, regular folic acid supplements and regular hematologic surveillance will suffice It will be important

to avoid exposure to potentially hemolytic drugs, and blood transfusion may be indicated when exacerbations occur, mostly in concomitance with intercurrent infection In rare patients, regular blood transfusions may be required; appropriate iron chelation should be instituted in such cases Unlike in hereditary spherocytosis, there is no evidence of selective red cell destruction in the spleen: however, in practice splenectomy has proven beneficial in severe cases

Other Abnormalities of the Redox System

As mentioned above, GSH is a key player in the defense against oxidative stress (Fig 101-4) Inherited defects of GSH metabolism are exceedingly rare, but each one of them can give rise to chronic HA (Table 101-4) A rare, peculiar,

usually self-limited severe HA of the first month of life, called infantile poikilocytosis, may be associated with deficiency of glutathione peroxidase

(GSHPx) due not to an inherited abnormality but to transient nutritional deficiency

of selenium, an element essential for the activity of GSHPx

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