Handbook of Microbiological Media, Fourth Edition part 194 doc

0 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.. 1.0g Preparation of Medium: Add components to distilled/deionized water and

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YC Medium without Tryptophan 1925

Preparation of Medium: Add components, except methanol, to

distilled/deionized water and bring volume to 1.0L Mix thoroughly

Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–

121°C Aseptically add filter-sterilized methanol Pour into sterile Petri

dishes or leave in tubes

Use: For the cultivation and maintenance of bacteria that can utilize

methanol as a carbon source, including Achromobacter

methanolo-phila, Methanomonas methylovora, Methylobacterium species, and

Pseudomonas methanolica.

YC Agar without Tryptophan

Compositionper liter:

Solution A 500.0mL

Solution B 500.0mL

Solution A:

Compositionper 500.0mL:

Glucose 20.0g

Succinic acid 10.0g

NaOH 6.0g

(NH4)2SO4 5.0g

KH2PO4 0.6g

MgSO4·7H2O 0.3g

Adenine 0.1g

L-Arginine 0.1g

L-Cysteine 0.1g

L-Leucine 0.1g

L-Lysine 0.1g

L-Threonine 0.1g

Uracil 0.1g

L-Aspartic acid 0.05g

L-Histidine 0.05g

L-Isoleucine 0.05g

L-Methionine 0.05g

L-Phenylalanine 0.05g

L-Proline 0.05g

L-Serine 0.05g

L-Tyrosine 0.05g

L-Valine 0.05g

NaCl 0.05g

CaCl2·2H2O 0.05g

DL-Methionine 0.01g

L-Histidine·HCl 0.005g

Inositol 1.0mg

KI 0.5mg

H3BO3 0.3mg

ZnSO4·7H2O 0.2mg

MnSO4·4H2O 0.2mg

Thiamine·HCl 0.2mg

Pyroxidine·HCl 0.2mg

Niacin 0.2mg

Calcium pantothenate 0.2mg

p-Aminobenzoic acid 0.1mg

Riboflavin 0.1mg

FeCl3 0.1mg

Na2MoO4·4H2O 0.1mg

CuSO4·5H2O 0.02mg

Folic acid 1.0μg

Biotin 1.0μg

pH 5.7 ± 0.2 at 25°C

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL Mix thoroughly Adjust pH to 5.6–5.7 Filter sterilize Warm to 50°–55°C

Solution B:

Agar, noble 20.0g

Preparation of Solution B: Add agar to distilled/deionized water and bring volume to 500.0mL Mix thoroughly Gently heat and bring

to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool to 50°–55°C

Preparation of Medium: Aseptically mix 500.0mL of solution A and 500.0mL of solution B Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Saccharomyces cerevi-siae.

YC Medium without Tryptophan

Solution A 500.0mL Solution B 500.0mL

Solution A:

Glucose 20.0g Succinic acid 10.0g NaOH 6.0g (NH4)2SO4 5.0g

KH2PO4 1.0g MgSO4·7H2O 0.5g NaCl 0.1g CaCl2·2H2O 0.1g Adenine 0.1g

L-Arginine 0.1g

L-Cysteine 0.1g

L-Leucine 0.1g

L-Lysine 0.1g

L-Threonine 0.1g Uracil 0.1g

L-Aspartic acid 0.05g

L-Histidine 0.05g

L-Isoleucine 0.05g

L-Methionine 0.05g

L-Phenylalanine 0.05g

L-Proline 0.05g

L-Serine 0.05g

L-Tyrosine 0.05g

L-Valine 0.05g Inositol 2.0mg

KI 1.0mg

H3BO3 0.5mg ZnSO4·7H2O 0.4mg MnSO4·4H2O 0.4mg Thiamine·HCl 0.4mg Pyroxidine·HCl 0.4mg Niacin 0.4mg Calcium pantothenate 0.4mg

Riboflavin 0.2mg FeCl3 0.2mg

Na2MoO4·4H2O 0.2mg

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1926 YDC Agar

CuSO4·5H2O 0.04mg

Folic acid 2.0μg

Biotin 2.0μg

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 500.0mL Adjust pH to 5.6–5.7 Filter

ster-ilize Warm to 50°–55°C

Solution B:

Agar, noble 20.0g

Preparation of Solution B: Add agar to distilled/deionized water

and bring volume to 500.0mL Gently heat and bring to boiling

Auto-clave for 15 min at 15 psi pressure–121°C Cool to 50–55°C

Preparation of Medium: Aseptically combine 500.0mL of

solu-tion A and 500.0mL of solusolu-tion B Pour into sterile Petri dishes or

dis-tribute into sterile tubes

Use: For the cultivation of Saccharomyces cerevisiae.

YDC Agar (Yeast Extract Dextrose Calcium Carbonate Agar)

CaCO3, finely divided 20.0g

Glucose 20.0g

Agar 15.0g

Yeast extract 10.0g

pH 7 0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes in 10.0mL volumes Autoclave for 15

min at 15 psi pressure–121°C Allow tubes to cool in a slanted position

Use: For the cultivation and maintenance of Pseudomonas species on

agar slants

YDC Medium

CaCO3 20.0g

Glucose 20.0g

Agar 15.0g

Yeast extract 10.0g

pH 7.2 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15

psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of Bdellovibrio species.

Yeast Agar, Van Niel’s

Agar 20.0g

Yeast extract 10.0g

K2HPO4 1.0g

MgSO4·7H2O 0.5g

pH 7.0–7.2 at 25°C

Preparation of Medium: Add components to tap water and bring

volume to 1.0L Mix thoroughly Gently heat and bring to boiling

Dis-tribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–

121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of a variety of microorganisms,

including Cytophaga species, Heliobacterium chlorum, Rhodomicrobium vannielii, Lysobacter enzymogenes, Rhodobacter species, Rhodocyclus gelatinosus, Rhodopseudomonas palustris, and Rhodospirillum rubrum.

Yeast Agar, Van Niel’s with Glutamate

Agar 20.0g Yeast extract 10.0g

K2HPO4 1.0g MgSO4 0.5g Monosodium glutamate 0.85g

pH 7.0–7.2 at 25°C

Preparation of Medium: Add components to tap water and bring volume to 1.0L Mix thoroughly Gently heat and bring to boiling Dis-tribute into tubes or flasks Autoclave for 15 min at 15 psi pressure– 121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of a variety of bacteria,

including Bacillus firmus, Cytophaga johnsonae, Heliobacterium chlorum, Lysobacter enzymogenes, Rhodobacter capsulatus, Rhodo-microbium vannielii, Rhodobacter sphaeroides, Rhodocyclus gelatino-sus, Rhodocyclus gelatinogelatino-sus, Rhodo-pseudomonas palustris, and Rhodospirillum rubrum.

Yeast Agar, Van Niel’s with 2.5% Sodium Chloride

(ATCC Medium 1370)

NaCl 25.0g Agar 20.0g Yeast extract 10.0g

K2HPO4 1.0g MgSO4 0.5g

pH 7.0–7.2 at 25°C

Use: For the cultivation and maintenance of Chromatium vinosum and Rhodopseudomonas species.

Yeast Agar, Van Niel’s with 25% Sodium Chloride

NaCl 250.0g Agar 20.0g Yeast extract 10.0g

K2HPO4 1.0g MgSO4 0.5g

pH 7.0–7.2 at 25°C

Use: For the cultivation and maintenance of halophilic bacteria, including Haloarcula vallismortis, Halococcus morrhuae, and Halo-bacterium species.

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Yeast Extract Agar 1927

Yeast Agar, Van Niel’s with Succinate

Agar 20.0g

Yeast extract 10.0g

Sodium succinate 1.35g

K2HPO4 1.0g

MgSO4·7H2O 0.5g

pH 7.0–7.2 at 25°C

Use: For the cultivation and maintenance of Rhodobacter capsulatus.

Yeast Ascospore Agar

Agar 30.0g

Potassium acetate 10.0g

Yeast extract 2.5g

Glucose 1.0g

psi pressure–121°C

Use: For the cultivation and observation of ascospore formation of

yeast

Yeast Beef Agar

See: Antibiotic Medium 4

Yeast Carbon Base, 10X (Wickerham Carbon Base Broth)

Glucose 10.0g

KH2PO4 1.0g

MgSO4·7H2O 0.5g

NaCl 0.1g

CaCl2·2H2O 0.1g

DL-Methionine 0.02g

DL-Tryptophan 0.02g

L-Histidine·HCl 0.01g

Inositol 2.0mg

H3BO3 0.5mg

ZnSO4·7H2O 0.4mg

MnSO4·4H2O 0.4mg

Thiamine·HCl 0.4mg

Pyridoxine 0.4mg

Niacin 0.4mg

Calcium pantothenate 0.4mg

Riboflavin 0.2mg

FeCl3 0.2mg

Na2MoO4·4H2O 0.2mg

KI 0.1mg

CuSO4·5H2O 0.04mg

Folic Acid 2.0μg

Biotin 2.0μg

pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Filter sterilize

Use: Used as a base to which different nitrogen sources may be added For the cultivation and differentiation of bacteria based on their ability

to utilize diverse added nitrogen sources

Yeast Dextrose Agar

Agar 15.0g Glucose 10.0g Yeast extract 10.0g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Adjust pH to 7.0 Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of a variety of heterotrophic microorganisms

Yeast Extract Agar

Agar 15.0g Malt extract 10.0g Glucose 4.0g Yeast extract 4.0g

pH 7.0 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the isolation and cultivation of Actinomadura species, Acti-nopolyspora species, Excellospora species, and Microspora species.

Agar 15.0g Peptone 5.0g Yeast extract 3.0g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath

Use: For the enumeration of microorganisms in potable and fresh water samples

Agar 15.0g Peptone 9.5g Yeast extract 7.0g Beef extract 5.0g NaCl 5.0g

pH 7.0 ± 0.2 at 25°C

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1928 Yeast Extract Agar

Use: For the cultivation of Aeromonas salmonicida.

Agar 20.0g

Yeast extract 1.0g

Buffer solution 2.0mL

pH 6.0 ± 0.2 at 25°C

Buffer Solution:

KH2PO4 60.0g

Na2HPO4 40.0g

Preparation of Buffer Solution: Add 40.0g of Na2HPO4 to

300.0mL of distilled/deionized water Mix thoroughly Add 60.0g of

KH2PO4 Mix thoroughly Adjust pH to 6.0

water and bring volume to 1.0L Mix thoroughly Autoclave for 15 min

at 15 psi pressure–121°C Pour into sterile Petri dishes

Use: For the identification of Histoplasma capsulatum, Blastomyces

dermatitidis, and Coccidioides immitis.

Agar 15.0g

Proteose peptone 10.0g

NaCl 5.0g

Yeast extract 3.0g

Use: For the cultivation of a variety of heterotrophic microorganisms

Yeast Extract Agar for Schizosaccharomyces

Glucose 30.0g

Agar 15.0g

Yeast extract 5.0g

Use: For the cultivation and maintenance of Schizosaccharomyces

japon-icus and Schizosaccharomyces pombe

Yeast Extract Beef Extract Medium

Yeast Extract Dextrose Calcium Carbonate Agar

Yeast Extract Glucose Agar

Agar 15.0g Glucose 15.0g

K2HPO4 5.2g

KH2PO4 3.18g

NH4Cl 0.54g Yeast extract 0.5g MgSO4 0.12g Trace elements solution 5.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

ZnSO4·7H2O 0.287g CuSO4·5H2O 0.249g MnSO4·4H2O 0.223g

Na2MoO4·2H2O 0.124g CaCl2·6H2O 0.118g KJ 0.083g

H3BO3 0.03g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.0 Gently heat and bring to boiling Distribute into tubes or flasks Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance ofBacillus licheniformis, Bacil-lus species, Clavibacter michiganense, Flavobacterium indologenes, Haf-nia alvei, Pseudomonas fluorescens, and Serratia marcescens.

Yeast Extract Glucose Broth

Glucose 15.0g

K2HPO4 5.2g

KH2PO4 3.18g

NH4Cl 0.54g Yeast extract 0.5g MgSO4 0.12g Trace elements solution 5.0mL

pH 7.0 ± 0.2 at 25°C

ZnSO4·7H2O 0.287g CuSO4·5H2O 0.249g MnSO4·4H2O 0.223g

Na2MoO4·2H2O 0.124g CaCl2·6H2O 0.118g KJ 0.083g

H3BO3 0.03g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.0 Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure– 121°C

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Yeast Extract Malt Extract Glucose Agar 1929

Use: For the cultivation and maintenance ofBacillus licheniformis,

Bacil-lus species, Clavibacter michiganense, Flavobacterium indologenes,

Haf-nia alvei, Pseudomonas fluorescens, and Serratia marcescens.

Yeast Extract Glucose Calcium Carbonate Agar

CaCO3 20.0g

Glucose 20.0g

Agar 15.0g

Yeast extract 10.0g

Use: For the isolation and cultivation of Erwinia species.

Yeast Extract Glucose Carbonate Medium

Yeast Extract Glucose Carbonate Peptone Medium

Yeast Extract Glucose Citrate Medium

Yeast Extract Glucose Citrate Medium with Cysteine

Yeast Extract Glucose Medium

Agar 15.0g

Yeast extract 10.0g

Glucose 10.0g

Use: For the cultivation of a variety of bacteria, including

Streptomy-ces species, Rhodococcus species, and others.

Yeast Extract Glycerol Medium

Agar 15.0g

Yeast extract 5.0g

Glycerol 50.0mL

Use: For the cultivation and maintenance of Geodermatophilus

obscu-rus subspecies utahensis.

Yeast Extract HiVeg Agar

Agar 15.0g

Plant peptone 5.0g

Yeast extract 3.0g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

Use: For the enumeration of microorganisms in potable and freshwa-ter samples A highly nutritive medium recommended for plate count

of microorganisms in water

Yeast Extract Malt Extract Agar

See: ISP Medium 2

Yeast Extract Malt Extract Agar, Diluted 1/10

Agar 20.0g Malt extract 1.0g Yeast extract 0.4g Glucose 0.4g

pH 7.3 ± 0.2 at 25°C

or leave in tubes

Use: For the cultivation and maintenance of Actinomadura atramen-taria, Microtetraspora africana, Parvopolyspora pallida, and Strep-tosporangium fragile.

Yeast Extract Malt Extract Glucose Agar

Agar 20.0g Glucose 10.0g Neopeptone 5.0g Malt extract 3.0g Yeast extract 3.0g

Use: For the isolation and cultivation of yeasts

Yeast Extract Malt Extract Glucose Agar

Agar 20.0g Malt extract 10.0g Glucose 4.0g Yeast extract 4.0g

pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Nocardia asteroides, Nocardia farcinica, Streptomyces antibioticus, Streptomyces argente-olus, Streptomyces aureofaciens, Streptomyces bluensis, Streptomyces caelestis, Streptomyces cinnamoneus, Streptomyces echinatus, Strep-tomyces griseocarneus, StrepStrep-tomyces griseus, StrepStrep-tomyces

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hawaiien-1930 Yeast Extract Mannitol Agar

sis, Streptomyces kanamyceticus, Streptomyces kentuckensis,

myces murinus, Streptomyces netropsis, Streptomyces niveus,

Strepto-myces nogalater, StreptoStrepto-myces nousei, StreptoStrepto-myces

paucisporo-genes, Streptomyces rimosus, Streptomyces sparsopaucisporo-genes,

Streptomy-ces spectabilis, StreptomyStreptomy-ces tendae, StreptomyStreptomy-ces tenebraruis,

Strep-tomyces violaceoruber, StrepStrep-tomyces viridifaciens, and Vibrio

salmo-nicida.

Yeast Extract Mannitol Agar

Agar 15.0g

Mannitol 10.0g

CaCO3 4.0g

K2HPO4 0.5g

Yeast extract 0.4g

MgSO4·7H2O 0.2g

NaCl 0.1g

pH 6.8–7.0 ± 0.2 at 25°C

water and bring volume to 1.0L Omit CaCO3 if a clear solution is

needed Mix thoroughly Gently heat and bring to boiling Distribute

into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of members of the rhizobiaceae

Yeast Extract Medium

Yeast extract 10.0g

Preparation of Medium: Add yeast extract to distilled/deionized

water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of Pseudomonas cepacia.

Yeast Extract Medium with Sodium Sulfide

Yeast extract 10.0g

Na2S 0.15g

Preparation of Medium: Add yeast extract to distilled/deionized

water and bring volume to 1.0L Mix thoroughly Autoclave for 15 min

at 15 psi pressure–121°C Immediately prior to inoculation, add 0.15g

of Na2S Mix thoroughly Aseptically distribute into sterile tubes or

flasks

Use: For the cultivation of Rhodospirillum molischianum.

Yeast Extract Mineral Agar

Agar 15.0g

Yeast extract 4.0g

NaHPO4·12H2O 3.5g

K2HPO4 1.0g

NH4Cl 0.5g

MgSO4·7H2O 0.03g

pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Bacillus azotoformans.

Yeast Extract Mineral Medium (DSMZ Medium 259)

Composition per liter:

Na2HPO4·12H2O 3.5g

K2HPO4 1.0g

NH4Cl 0.5g MgSO4·7H2O 0.03g

pH 7.1 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Bacillus azotoformans.

Yeast Extract Nutrient Agar Medium

Yeast Extract Nutrient Gelatin Medium

Yeast Extract Peptone Beef Extract Medium

Yeast Extract Peptone Starch Agar

Agar 18.0g Soluble starch 10.0g Peptone 10.0g CaCO3 5.0g Sodium acetate 5.0g Yeast extract 3.0g

KH2PO4 0.5g

K2HPO4 0.5g MgSO4·7H2O 0.3g Sodium citrate 0.027g NaCl 0.01g MnSO4·5H2O 0.01g CuSO4·5H2O 1.0mg CoCl2·6H2O 1.0mg FeSO4·7H2O 1.0mg

Use: For the cultivation and maintenance of Bacillus species that

uti-lize starch as a carbon source

Yeast Extract Peptone Sulfate Cysteine Medium

Yeast Extract Phosphate Agar

(YEP Agar)

Composition per liter:

KH2PO4 0.3g

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Yeast Extract Sucrose Agar 1931

Na2HPO4 0.2g

Phenol Red 1.0mg

Source: This medium is available as a premixed powder from BD

Di-agnostic Systems

Use: For the isolation of dimorphic pathogenic fungi from clinical

specimens

Yeast Extract Proteose Peptone Medium

Yeast Extract Rose Bengal HiVeg Broth Base

with Sorbose

Na2HPO4 17.25g

Yeast extract 5.0g

Synthetic detergent 2.0g

NaCl 1.0g

Sodium pyruvate 1.0g

Rose Bengal 0.04g

MgSO4 0.01g

Sorbose solution 100.0mL

pH 7.9 ± 0.2 at 25°C

Source: This medium, without sorbose solution, is available as a

pre-mixed powder from HiMedia

Sorbose Solution:

Sorbose 4.0g

Preparation of Sorbose Solution: Add sorbose to

distilled/deion-ized water and bring volume to 100.0mL Filter sterilize

Preparation of Medium: Add components, except sorbose

solu-tion, to distilled/deionized water and bring volume to 900.0mL Mix

thoroughly Gently heat and bring to boiling Autoclave for 15 min at

15 psi pressure–121°C Cool to 45–-50°C Aseptically add 100.0mL of

sterile sorbose solution Mix thoroughly Pour into sterile Petri dishes

or distribute into sterile tubes

Use: For the cold enrichment for recovery of Yersinia enterocolitica

and Yersinia pseudotuberculosis from foods

Yeast Extract Skim Milk Agar

Agar 15.0g

Skim milk powder 10.0g

Yeast extract 1.0g

Use: For the cultivation of Lysobacter enzymogenes.

Yeast Extract Sodium Lactate Medium

Agar 15.0g

Pancreatic digest of casein 10.0g Yeast extract 10.0g Sodium lactate 10.0g

KH2PO4 2.5g MnSO4 5.0mg

pH 7.0 ± 0.2 at 25°C

Use: For the isolation, cultivation, and maintenance of Propionibacte-rium species.

Yeast Extract Sucrose Agar

(YESA) (ATCC Medium 2125)

Sucrose 20.0g Agar 15.0g Yeast extract 4.0g

KH2PO4 1.0g MgSO4·7H2O 0.5g

pH 6.2 ± 0.2 at 25°C

or leave in tubes

Use: For the cultivation and maintenance of various fungi

Yeast Extract Sucrose Agar

Sucrose 150.0g Agar 20.0g Yeast extract 20.0g MgSO4·7H2O 0.5g Trace metals solution 1.0mL

pH 6.5 ± 0.2 at 25°C

Trace Metals Solution:

ZnSO4·7H2O 1.0g CuSO4·5H2O 0.5g

Preparation of Trace Metals Solution: Add components to dis-tilled/deionized water and bring volume to 100.0mL Mix thoroughly

or leave in tubes

Use: For the cultivation and maintenance of various fungi

Yeast Extract Tryptone Medium

Yeast Extract Tryptone NaCl Medium

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1932 Yeast Fermentation Broth

Yeast Fermentation Broth

Carbohydrate 10.0g

Pancreatic digest of gelatin 7.5g

Yeast extract 5.5g

Bromcresol Purple 16.0mg

Di-agnostic Systems

water and bring volume to 1.0L Mix thoroughly Distribute into test

tubes, each containing an inverted Durham tube Autoclave for 15 min

at 15 psi pressure–121°C

Use: For fermentation tests of specific carbohydrates used in the

char-acterization and identification of yeasts Gas accumulation in the

Dur-ham tube and a color change of the medium to yellow indicates

carbo-hydrate fermentation

Yeast Fermentation HiVeg Broth Base

with Carbohydrate (Bromcresol Purple HiVeg Broth Base)

Plant peptone 10.0g

NaCl 5.0g

Plant extract 3.0g

Bromcresol Purple 0.04g

Carbohydrate solution 50.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium, without carbohydrate solution, is available as

a premixed powder from HiMedia

Carbohydrate Solution:

Carbohydrate 10.0g

Preparation of Carbohydrate Solution: Add carbohydrate to

distilled/deionized water and bring volume to 100.0mL Adonitol,

ara-binose, cellobiose, glucose, dulcitol, fructose, galactose, inositol,

lac-tose, mallac-tose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose,

trehalose, xylose, or other carbohydrates may be used Mix thoroughly

Filter sterilize

Preparation of Medium: Add components, except carbohydrate

solution, to distilled/deionized water and bring volume to 1.0L Mix

thoroughly Gently heat and bring to boiling Distribute in 10.0mL

vol-umes into test tubes containing inverted Durham tubes Autoclave for

15 min at 15 psi pressure–121°C Cool to 25°C Add 0.5mL of sterile

carbohydrate solution to each tube

Use: For the determination of carbohydrate fermentation reactions of

microorganisms

Yeast Fermentation Medium

Peptone 7.5g

Yeast extract 4.5g

Bromthymol Blue (1.6% solution) 1.0mL

Carbohydrate solution 1.0mL

Carbohydrate Solution:

Carbohydrate 0.6g

Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 10.0mL Glucose, maltose, lactose, galactose, or trehalose may be used If raffinose is used, prepare

a 12% solution Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring to boiling Distribute in 2.0mL vol-umes into test tubes that contain an inverted Durham tube Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 1.0mL of sterile carbohydrate solution Mix thoroughly

Use: For the cultivation and differentiation of yeast based on carbohy-drate fermentation patterns Yeasts that can ferment a specific carbo-hydrate turn the medium yellow

Yeast Glucose Agar

Composition per liter:

Agar 15.0g Pancreatic digest of gelatin 7.75g Beef extract 4.75g Yeast extract 2.5g

K2HPO4 2.5g Glucose 1.0g

pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a wide variety of bacteria

Yeast Glucose Agar

Glucose 20.0g Agar 15.0g Yeast extract 10.0g

pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Acetobacter aceti, Acetobacter hansenii, Ace-tobacter liquefaciens, AceAce-tobacter pasteurianus, AceAce-tobacter species, Brevibacterium species, Dermabacter hominus, Clostridium saccharoper-butylacetonicum, Corynebacterium amycolatum, Gluconobacter asaii, Gluconobacter cerinus, Gluconobacter frateurii, Gluconobacter oxydans, Gluconobacter species, Lactococcus piscium, and Streptococcus mutans.

Yeast Glucose Agar for Acetobacter

Glucose 100.0g CaCO3 20.0g Agar 15.0g Yeast extract 10.0g

pH 7.0 ± 0.2 at 25°C

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Yeast Malate Medium 1933

Use: For the cultivation and maintenance ofAcetobacter aceti,

Aceto-bacter pasteurianus, and GluconoAceto-bacter oxydans.

Yeast Glucose Broth

Pancreatic digest of gelatin 7.75g

Beef extract 4.75g

Yeast extract 2.5g

K2HPO4 2.5g

Glucose 1.0g

pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Staphylococcus caseolyticus.

Yeast Glucose Broth

Glucose 20.0g

Yeast extract 10.0g

Use: For the cultivation of Enterococcus mundtii, Enterococcus

pseudo-avium, Enterococcus raffinosus, Enterococcus solitarius, Enterococcus

sulfureus, Lactococcus raffinolactis, and Vagococcus fluvialis.

Yeast Glucose Broth (YGB)

Beef extract 10.0g

Peptone 10.0g

NaCl 5.0g

Glucose 5.0g

Yeast extract 3.0g

pH 6.8 ± 0.2 at 25°C

water and bring volume to 1.0L Mix thoroughly Adjust pH to 6.8

121°C

Use: For the cultivation of Enterococcus faecalis, Streptococcus

angi-nosus, and Rhodobacter sphaeroides.

Yeast Glucose Litmus Milk

Yeast Glucose Litmus Milk with Chalk

Yeast HiVeg Agar (Antibiotic HiVeg Assay Medium No 4)

Agar 15.0g

Plant peptone 6.0g

Yeast extract 3.0g

Plant extract 1.5g Glucose 1.0g

pH 6.6 ± 0.05 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

Use: For antibiotic assay testing

Yeast Malate Medium

Yeast extract 5.0g Sodium malate 1.0g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into tubes

Use: For the cultivation of Rhodopseudomonas viridis.

Yeast Malate Medium

KH2PO4·12H2O 1.0g NaHCO3 1.0g Sodium malate 1.0g (NH4)2SO4 0.5g Trace elements solution 1.0mL

pH 6.8 ± 0.2 at 25°C

H3BO3 0.3g CoCl2·6H2O 0.2g ZnSO4·7H2O 0.1g MnCl2·4H2O 0.03g

Na2MoO4·2H2O 0.03g NiCl2·6H2O 0.02g CuCl2·2H2O 0.01g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except trace elements solution, to distilled/deionized water and bring volume to 999.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Asep-tically add 1.0mL of sterile trace elements solution Mix thoroughly Aseptically distribute into sterile tubes or flasks

Use: For the cultivation of Rhodopseudomonas viridis.

Yeast Malate Medium (LMG 176)

KH2PO4·12H2O 1.0g NaHCO3 1.0g Sodium malate 1.0g Yeast extract 1.0g (NH4)2SO4 0.5g Trace elements solution 1.0mL

pH 6.8–7.0 at 25°C

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1934 Yeast Malt Agar

H3BO3 0.3g

CoCl2·6H2O 0.2g

ZnSO4·7H2O 0.1g

MnCl2·4H2O 0.03g

Na2MoO4·2H2O 0.03g

NiCl2·6H2O 0.02g

CuCl2·2H2O 0.01g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L Mix thoroughly

water and bring volume to 1.0L Mix thoroughly Adjust pH to 6.8–7.0

121°C

Use: For the cultivation of Rhodopseudomonas viridis

Yeast Malt Agar

Agar 20.0g

Malt extract 10.0g

Glucose 4.0g

Yeast extract 4.0g

pH 7.2 ± 0.2 at 25°C

to boiling Adjust pH to 7.2 Distribute into tubes or flasks Autoclave

for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or

leave in tubes

Use: For the cultivation and maintenance of actinomycetes, yeasts,

and fungi

Yeast Malt Agar for Arthrobacter viscosus

NaCl 10.0g

Pancreatic digest of casein 10.0g

Yeast extract 5.0g

pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Arthrobacter viscosus and Escherichia coli.

Yeast Malt Extract Agar (YM Agar)

Agar 20.0g

Glucose 10.0g

Peptone 5.0g

Yeast extract 3.0g

Malt extract 3.0g

pH 6.2 ± 0.2 at 25°C

Di-agnostic Systems

psi pressure–121°C The medium may be rendered selective by

adjust-ing the pH to 3.0–4.0 at 45°–55°C or by the addition of antibiotics at 45°–50°C or below Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of fungi, including yeasts, and other aciduric

microorganisms such as Actinoplanes species, Streptomyces species, Streptoverticillium species, and Nocardia species.

Yeast Malt Extract Broth (YM Broth)

Glucose 10.0g Peptone 5.0g Yeast extract 3.0g Malt extract 3.0g

pH 6.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems

or flasks Autoclave for 15 min at 15 psi pressure–121°C The medium may be rendered selective by adjusting the pH to 3.0–4.0 at 45°–55°C

or by the addition of antibiotics at 45°–50°C or below

Use: For the cultivation of yeasts, molds, and other aciduric

microor-ganisms such as Actinoplanes species, Streptomyces species, Strep-toverticillium species, and Nocardia species.

Yeast Malt Extract Broth with 0.5% Calcium Carbonate

Glucose 10.0g Peptone 5.0g CaCO3 5.0g Yeast extract 3.0g Malt extract 3.0g

pH 6.2 ± 0.2 at 25°C

or flasks Autoclave for 15 min at 15 psi pressure–121°C The medium may be rendered selective by adjusting the pH to 3.0–4.0 at 45–55°C

or by the addition of antibiotics at 45–50°C or below

Use: For the cultivation and maintenance of Dekkera anomala, Dekkera bruxellensis, Dekkera claussenii, and Dekkera lambica.

Yeast Malt Extract Broth with 2.0% Calcium Carbonate

CaCO3 20.0g Glucose 10.0g Peptone 5.0g Yeast extract 3.0g Malt extract 3.0g

pH 6.2 ± 0.2 at 25°C

or flasks Autoclave for 15 min at 15 psi pressure–121°C The medium

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