Handbook of Microbiological Media, Fourth Edition part 154 ppsx

0.01g Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L.. 0.02g Preparation of Medium: Add components to distilled/deionized wa

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S Medium 1525

Ryan’s Aeromonas Medium

See: Aeromonas Medium

R2YE Medium

Compositionper 1062.2mL:

Thiostrepton 50.0mg

Basal solution 800.0mL

TES (N-tris[hydroxymethyl]methyl-2-amino–ethane-

sulfonic acid) buffer 100.0mL

CaCl2·2H2O solution 80.2mL

Yeast extract solution 50.0mL

L-Proline solution 15.0mL

KH2PO4 solution 10.0mL

NaOH solution 5.0mL

Trace elements solution 2.0mL

pH 7.2 ± 0.2 at 25°C

Basal Solution:

Sucrose 103.0g

MgCl2·6H2O 10.12g

D-Glucose 10.0g

K2SO4 0.25g

Casamino acids 0.1g

Preparation of Basal Solution: Add components to

distilled/de-ionized water and bring volume to 800.0mL Mix thoroughly

Auto-clave for 15 min at 15 psi pressure–121°C Cool to 25°C

TES Buffer:

Composition per liter:

TES (N-tris[hydroxymethyl]

methyl-2-amino–ethane-

sulfonic acid) buffer 57.3g

Preparation of TES Buffer: Add TES to distilled/deionized water

and bring volume to 1.0L Mix thoroughly Adjust pH to 7.2 Filter

sterilize

CaCl 2 ·2H 2 O Solution:

Composition per 100.0mL:

CaCl2·2H2O 3.68g

Preparation of CaCl 2 ·2H 2 O Solution: Add the CaCl2·2H2O to

distilled/deionized water and bring volume to 100.0mL Mix

thorough-ly Filter sterilize

Yeast Extract Solution:

Yeast extract 10.0g

Preparation of Yeast Extract Solution: Add yeast extract to

dis-tilled/deionized water and bring volume to 100.0mL Mix thoroughly

Filter sterilize

L - Proline Solution:

L-Proline 20.0g

Preparation of L- Proline Solution: Add the proline to distilled/

deionized water and bring volume to 100.0mL Mix thoroughly Filter

sterilize

KH 2 PO 4 Solution:

KH2PO4 0.5g

Preparation of KH 2 PO 4 Solution Solution: Add the KH2PO4 to distilled/deionized water and bring volume to 100.0mL Mix

NaOH Solution:

NaOH 40.0g

Preparation of NaOH Solution: Add the NaOH to distilled/deion-ized water and bring volume to 100.0mL Mix thoroughly Filter ster-ilize

Trace Elements Solution:

FeCl3·6H2O 0.2g ZnCl2 0.04g CuCl2·2H2O 0.01g MnCl2·4H2O 0.01g

Na2B4O7·10H2O 0.01g (NH4)6Mo7O24·4H2O 0.01g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Filter sterilize

Preparation of Medium: To 800.0mL of cooled, sterile basal solu-tion, aseptically add the remaining components Mix thoroughly Asep-tically distribute into sterile tubes or flasks

Use: For the cultivation and maintenance of Streptomyces lividans.

S aureus ID

Compositionper liter:

Proprietary

Source: This medium is available from bioMérieux

Use: For the direct identification of Staphylococcus aureus and the se-lective isolation of staphylococci Direct identification of S aureus is

based on the spontaneous green coloration of α-glucosidase-producing colonies

S Broth

Peptone 10.0g Meat extract 2.4g NaCl 2.0g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of Bacillus cereus.

S Medium

Glycogen 3.0g MgSO4·7H2O 2.0g

L-Leucine 1.0g

L-Tyrosine 0.6g

L-Asparagine 0.5g

L-Isoleucine 0.5g

L-Proline 0.5g

L-Lysine 0.25g

KH2PO4 0.13g

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1526 S Salts

Djenkolic acid 0.1g

L-Arginine 0.1g

L-Serine 0.1g

L-Threonine 0.1g

L-Valine 0.1g

L-Alanine 0.05g

L-Glycine 0.05g

L-Histidine 0.05g

L-Methionine 0.05g

L-Tryptophan 0.05g

pH 7.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L Mix thoroughly Filter sterilize

Asep-tically distribute into tubes or flasks

Use: For the cultivation of Myxococcus xanthus.

S Salts

Dibenzothiophene 5.0g

NH4Cl 0.5g

KH2PO4 0.25g

MgCl2·6H2O 0.25g

pH 6.5–7.0 at 25°C

water and bring volume to 1.0L Mix thoroughly Adjust pH to 6.5–7.0

with KOH Distribute into tubes or flasks Autoclave for 15 min at 15

psi pressure–121°C

Use: For the cultivation of Bacillus sulfasportare.

S6 Medium for Thiobacilli

Agar 15.0g

Na2S2O3 10.0g

KH2PO4 11.8g

Na2HPO4 1.2g

MgSO4·7H2O 0.1g

(NH4)2SO4 0.1g

CaCl2 0.03g

FeCl3 0.02g

MnSO4 0.02g

water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15

psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Thiobacillus denitrificans

and Thiobacillus thioparus.

S8 Medium for Thiobacilli

Agar 15.0g

KH2PO4 11.8g

Na2S2O3 10.0g

KNO3 5.0g

Na2HPO4 1.2g

NaHCO3 0.5g

MgSO4·7H2O 0.1g

(NH4)2SO4 0.1g

CaCl2 0.03g

FeCl3 0.02g MnSO4 0.02g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Thiobacillus neapolita-nus

SA Agar

Agar 15.0g Pancreatic digest of casein 10.0g NaCl 5.0g Starch, soluble 1.0g Ampicillin 0.01g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except ampicillin, to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pres-sure–121°C Cool to 45°–50°C Aseptically add ampicillin Mix thor-oughly Pour into sterile Petri dishes

Use: For the isolation, cultivation, and differentiation, based on starch

hydrolysis, of Aeromonas hydrophila from foods After inoculation of

plates and growth of cultures, starch hydrolysis is determined by flood-ing each plate with 5.0mL of Lugol’s iodine solution

SA Agar, Modified (Lachica’s Medium)

Beef heart, solids from infusion 500.0g Agar 15.0g Tryptose 10.0g NaCl 5.0g Amylose Azure 3.0g Ampicillin 0.01mg

pH 7.4 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

Use: For the isolation and cultivation of Aeromonas hydrophila from foods Aeromonas hydrophila appears as colonies surrounded by a

light halo on a light blue background

SA HiVeg Agar Base with Ampicillin

Agar 15.0g Plant hydrolysate 10.0g Starch, soluble 10.0g NaCl 5.0g Phenol Red 0.025g Ampicillin solution 10.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium, without ampicillin, is available as a premixed powder from HiMedia

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SABHI Blood Agar 1527

Ampicillin Solution:

Ampicillin 0.01g

Preparation of Ampicillin Solution: Add ampicillin to distilled/

deionized water and bring volume to 10.0mL Mix thoroughly Filter

sterilize

Preparation of Medium: Add components, except ampicillin

solu-tion, to distilled/deionized water and bring volume to 990.0mL Mix

thoroughly Gently heat and bring to boiling Autoclave for 15 min at

15 psi pressure–121°C Cool to 45°C Aseptically add sterile

ampicil-lin solution Mix thoroughly Pour into sterile Petri dishes or distribute

into sterile tubes

Use: For the isolation, cultivation, and differentiation of Aeromonas

hydrophilia from foods based on starch hydrolysis.

SABHI Agar (Sabouraud Glucose and Brain Heart Infusion Agar)

Glucose 21.0g

Agar 15.0g

Pancreatic digest of casein 10.5g

Peptic digest of animal tissue 5.0g

Brain heart, solids from infusion 4.0g

NaCl 2.5g

Na2HPO4 1.25g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD

Di-agnostic Systems

psi pressure–121°C Pour into sterile Petri dishes in 20.0mL volumes

or leave in tubes

Use: For the cultivation of dermatophytes and other pathogenic and

nonpathogenic fungi from clinical and nonclinical specimens

SABHI Agar

Beef heart, infusion from 125.0g

Calf brains, infusion from 100.0g

Glucose 21.0g

Agar 15.0g

Neopeptone 5.0g

Proteose peptone 5.0g

NaCl 2.5g

Na2HPO4 1.25g

Chloromycetin solution 1.0mL

pH 7.0 ± 0.2 at 25°C

Di-agnostic Systems

Chloromycetin Solution:

Chloromycetin 1.0g

Preparation of Chloromycetin Solution: Add chloromycetin to

Preparation of Medium: Add components, except chloromycetin solution, to distilled/deionized water and bring volume to 999.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min

at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 1.0mL of sterile chloromycetin solution Mix thoroughly Aseptically distribute into sterile tubes in 5.0mL volumes

Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens

SABHI Agar, Modified

Beef heart, infusion from 62.5g Calf brain, infusion from 50.0g Glucose 20.5g Brain heart infusion broth 18.6g Agar 7.5g Neopeptone 5.0g Pancreatic digest of gelatin 2.5g NaCl 1.25g

Na2HPO4 0.625g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Dissolve, then autoclave at 121°C for 15 min Cool to 50°C and add 1.0mL of sterile chloramphenicol solution (100.0mg/mL) Mix well and dispense into sterile tubes Slant and al-low to harden Refrigerate until needed

SABHI Blood Agar

Beef heart, infusion from 125.0g Calf brains, infusion from 100.0g Glucose 21.0g Agar 15.0g Neopeptone 5.0g Proteose peptone 5.0g NaCl 2.5g

Na2HPO4 1.25g Blood 100.0mL Chloromycetin solution 1.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems

Chloromycetin 1.0g

Preparation of Chloromycetin Solution: Add chloromycetin to distilled/deionized water and bring volume to 10.0mL Mix

Preparation of Medium: Add components, except blood and chloro-mycetin solution, to distilled/deionized water and bring volume to 899.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for

15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 100.0mL of sterile blood and 1.0mL of sterile chloromycetin solution Sheep blood or human blood may be used Mix thoroughly Aseptically distribute into sterile tubes in 5.0mL volumes

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1528 SABHI HiVeg Agar Base with Chloramphenicol

nonpathogenic fungi from clinical and nonclinical specimens Blood

enhances the recovery of Blastomyces dermatitidis and Histoplasma

capsulatum and their conversion to the yeast phase.

SABHI HiVeg Agar Base with Chloramphenicol

Glucose 21.0g

Agar 15.0g

Plant infusion 5.14g

Plant peptone No 3 5.0g

Plant special peptone 5.0g

Plant special infusion 4.11g

NaCl 2.5g

Na2HPO4 1.25g

Chloramphenicol solution 10.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium, without chloramphenicol, is available as a

pre-mixed powder from HiMedia

Chloramphenicol Solution:

Chloramphenicol 0.1g

Preparation of Chloramphenicol Solution: Add

chlorampheni-col to distilled/deionized water and bring volume to 10.0mL Mix

thor-oughly Filter sterilize

Preparation of Medium: Add components, except

chlorampheni-col solution, to distilled/deionized water and bring volume to 990.0mL

Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min

at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 10.0mL

of sterile chloramphenicol solution Mix thoroughly Pour into Petri

dishes or aseptically distribute into sterile tubes

nonpathogenic fungi from clinical and nonclinical specimens

SABHI HiVeg Agar Base with Chloromycetin

Glucose 21.0g

Agar 15.0g

Plant infusion 5.14g

Plant peptone No 3 5.0g

Plant special infusion 4.11g

NaCl 2.5g

Na2HPO4 1.25g

Chloromycetin solution 1.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium, without chloromycetin, is available as a

pre-mixed powder from HiMedia

Chloromycetin 1.0g

Preparation of Chloromycetin Solution: Add chloromycetin to

Preparation of Medium: Add components, except chloromycetin

solution, to distilled/deionized water and bring volume to 999.0mL

Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min

at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 1.0mL of

sterile chloromycetin solution Mix thoroughly Aseptically distribute into sterile tubes in 5.0mL volumes

SABHI HiVeg Agar Base with Blood and

Chloromycetin

Glucose 21.0g Agar 15.0g Plant infusion 5.14g Plant peptone No 3 5.0g Plant special peptone 5.0g Plant special infusion 4.11g NaCl 2.5g

Na2HPO4 1.25g Blood 100.0mL Chloromycetin solution 1.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium, without blood and chloromycetin, is available

as a premixed powder from HiMedia

Chloromycetin 1.0g

Preparation of Chloromycetin Solution: Add chloromycetin to distilled/deionized water and bring volume to 10.0mL Mix

Preparation of Medium: Add components, except blood and chloro-mycetin solution, to distilled/deionized water and bring volume to 899.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for

15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 100.0mL of sterile blood and 1.0mL of sterile chloromycetin solution Sheep blood or human blood may be used Mix thoroughly Aseptically distribute into sterile tubes in 5.0mL volumes

Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens Blood

enhances the recovery of Blastomyces dermatitidis and Histoplasma capsulatum and their conversion to the yeast phase.

Sabouraud Agar

Neopeptone 30.0g Agar 20.0g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to tap water and bring volume to 1.0L Mix thoroughly Gently heat and bring to boiling Dis-tribute into tubes or flasks Autoclave for 15 min at 15 psi pressure– 121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of yeasts and molds

Sabouraud Agar with CCG and 3% Sodium Chloride

Glucose 120.0g NaCl 90.0g Agar 45.0g Peptone 30.0g Chloramphenicol solution 15.0mL

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Sabouraud Agar, Modified 1529

Cycloheximide solution 15.0mL

Gentamicin solution 1.5mL

Cycloheximide Solution:

Cycloheximide 0.3g

Preparation of Cycloheximide Solution: Add cycloheximide to

Caution: Cycloheximide is toxic Avoid skin contact or aerosol

for-mation and inhalation

Gentamicin Solution:

Gentamicin 0.4g

Preparation of Gentamicin Solution: Add gentamicin to

dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly

Filter sterilize

Preparation of Medium: Add components—except

chloramphen-icol solution, cycloheximide solution, and gentamicin solution—to

dis-tilled/deionized water and bring volume to 3.0L Mix thoroughly

Gently heat and bring to boiling Autoclave for 15 min at 15 psi

pres-sure–121°C Cool to 45°–50°C Aseptically add 15.0mL of sterile

chloramphenicol solution, 15.0mL of sterile cycloheximide solution,

and 1.5mL of sterile gentamicin solution Mix thoroughly Aseptically

distribute into sterile tubes Allow tubes to cool in a slanted position

Use: For the selective isolation and cultivation of fungi from

speci-mens with a mixed flora

Sabouraud Agar with CCG and 5% Sodium Chloride

NaCl 150.0g

Glucose 120.0g

Agar 45.0g

Peptone 30.0g

Gentamicin solution 1.5mL

Cycloheximide 0.3g

Gentamicin Solution:

Gentamicin 0.4g

Preparation of Gentamicin Solution: Add gentamicin to dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components—except chloramphen-icol solution, cycloheximide solution, and gentamicin solution—to dis-tilled/deionized water and bring volume to 3.0L Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pres-sure–121°C Cool to 45°–50°C Aseptically add 15.0mL of sterile chloramphenicol solution, 15.0mL of sterile cycloheximide solution, and 1.5mL of sterile gentamicin solution Mix thoroughly Aseptically distribute into sterile tubes Allow tubes to cool in a slanted position

Use: For the selective isolation and cultivation of fungi from speci-mens with a mixed flora

Sabouraud Agar, Diluted 1/10

Agar 20.0g Glucose 4.0g

KH2PO4 1.5g MgSO4·7H2O 1.0g NaNO3 1.0g Peptone 1.0g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 6.8 Gently heat and bring to boiling Distribute into tubes or flasks Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance of a variety of fungi and

het-erotrophic bacteria.

Sabouraud Agar, Diluted 1/10 with Salt

Agar 15.0g Glucose 2.0g

KH2PO4 1.0g MgSO4·7H2O 1.0g Mycological peptone 1.0g

pH 6.8–7.0 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 6.8–7.0 Gently heat and bring to boiling Distribute into tubes or flasks Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance ofArthroderma benhamiae, Arthroderma vanbreuseghemii, Microsporum canis, and Trichophyton mentagrophytes.

Sabouraud Agar, Modified

Agar 20.0g Glucose 20.0g Neopeptone 10.0g

pH 7.0 ± 0.2 at 25°C

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1530 Sabouraud Chloramphenicol HiVeg Agar

Di-agnostic Systems

Use: For the cultivation of yeasts, molds, and aciduric bacteria

Sabouraud Chloramphenicol HiVeg Agar

Glucose 40.0g

Agar 15.0g

Plant hydrolysate 5.0g

Plant peptone 5.0g

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from

Hi-Media

Use: For the cultivation and identification of yeasts

Sabouraud Cycloheximide Chloramphenicol

HiVeg Agar

Glucose 20.0g

Agar 15.0g

Plant peptone 10.0g

Cycloheximide 0.5g

pH 6.8 ± 0.2 at 25°C

Hi-Media

Caution: Cycloheximide is very toxic Avoid skin contact or aerosol

formation and inhalation

Use: For the isolation and cultivation of pathogenic fungi

Sabouraud Dextrose Agar

(BAM M133)

Neopeptone 30.0g

Agar 20.0g

pH 5.6 ± 0.2 at 25°C

Preparation of Medium: Add components to tap water and bring

volume to 1.0L Mix thoroughly Gently heat and bring to boiling

Dis-tribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–

121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of yeasts and molds

Sabouraud Dextrose Agar pH 5.6

Glucose 40.0g Agar 15.0g Mycological peptone 10.0 g

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat while stirring and bring to boiling Autoclave for 15 min at 15 psi pressure– 121°C Cool to 50°C Mix thoroughly Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of yeasts and other fungi For use in qualita-tive and quantitaqualita-tive procedures for yeasts and fungi

Sabouraud Glucose Agar (Saboraud Dextrose Agar) (SabDex, 2%)

Glucose 20.0g Agar 15.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g

pH 5.6 ± 0.2 at 25°C

Use: For the cultivation of yeast and filamentous fungi For the culti-vation of pathogenic and nonpathogenic fungi, especially dermato-phytes The medium may be made more selective for fungi by the addi-tion of chloramphenicol Fluconozole (final concentraaddi-tion of 8–16mg per mL) may also be added to test for antibiotic sensitivity

Sabouraud Glucose Agar (Saboraud Dextrose Agar) (SabDex, 4%)

Glucose 40.0g Agar 15.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems and Oxoid Unipath

Use: For the cultivation of yeast and filamentous fungi For the culti-vation of pathogenic and nonpathogenic fungi, especially dermato-phytes The medium may be made more selective for fungi by the addi-tion of chloramphenicol Fluconozole (final concentraaddi-tion of 8–16mg per mL) may also be added to test for antibiotic sensitivity

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Sabouraud Glucose Broth 1531

Sabouraud Glucose Agar, Emmons

Glucose 20.0g

Agar 17.0g

pH 6.9 ± 0.2 at 25°C

Di-agnostic Systems

nonpathogenic fungi from clinical and nonclinical specimens For the

cultivation of yeast and filamentous fungi

Sabouraud Glucose Agar with

Chloramphenicol and Cycloheximide

Glucose 40.0g

Agar 15.0g

pH 5.6 ± 0.2 at 25°C

Cycloheximide 0.5g

Acetone 10.0mL

acetone Mix thoroughly

Ethanol (95% solution) 10.0mL

chlorampheni-col to ethanol Mix thoroughly

Preparation of Medium: Add components, except cycloheximide

solution and chloramphenicol solution, to distilled/deionized water and

bring volume to 980.0mL Mix thoroughly Gently heat and bring to

boiling Add the cycloheximide solution and chloramphenicol

solu-tion Mix thoroughly Distribute into tubes or flasks Autoclave for 15

min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in

tubes

Use: For the cultivation and identification of yeasts

Sabouraud Glucose Agar, HiVeg

Glucose 40.0g

Agar 15.0g

Plant peptone No 4 10.0g Selective supplement 10.0mL

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

Selective Supplement:

Cycloheximide 0.5g Chloramphenicol 0.04g

Preparation of Selective Supplement: Add components to dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly Filter sterilize

Caution: Cycloheximide is very toxic Avoid skin contact or aerosol formation and inhalation

Preparation of Medium: Add components, except selective sup-plement, to distilled/deionized water and bring volume to 990.0mL Mix thoroughly Gently heat and bring to boiling Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C Cool to 50°C Aseptically add 10.0mL sterile selective supplement Mix thor-oughly Pour into sterile Petri dishes or aseptically distribute into tubes

Use: For the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens For the cultivation of yeast and filamentous fungi

Sabouraud Glucose Agar with Olive Oil

Glucose 40.0g Agar 15.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Olive oil 20.0mL Tween™ 80 2.0mL

pH 5.6 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Allow tubes to cool in a slanted position

Use: For the cultivation and maintenance of Malassezia species.

Sabouraud Glucose and Brain Heart Infusion Agar

See: SABHI Agar

Sabouraud Glucose Broth

Glucose 20.0g Neopeptone 10.0g

pH 5.6 ± 0.2 at 25°C

or flasks Autoclave for 15 min at 15 psi pressure–121°C Avoid over-heating

Use: For the cultivation of pathogenic and nonpathogenic fungi, espe-cially dermatophytes The medium may be made more selective for

fungi by the addition of chloramphenicol

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1532 Sabouraud Glucose HiVeg Agar Base, Modified with Cycloheximide and Chloramphenicol

Sabouraud Glucose HiVeg Agar Base, Modified

with Cycloheximide and Chloramphenicol

(Glucose HiVeg Agar Base, Emmons)

Glucose 20.0g

Agar 17.0g

pH 7.0 ± 0.2 at 25°C

Hi-Media

Use: For the cultivation of fungi

Sabouraud Glucose HiVeg Broth

(Sabouraud Liquid HiVeg Medium)

Glucose 20.0g

pH 5.6 ± 0.2 at 25°C

Hi-Media

water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of pathogenic and nonpathogenic fungi,

espe-cially dermatophytes The medium may be made more selective for

fungi by the addition of chloramphenicol

Sabouraud Glucose Maltose HiVeg Agar

Agar 15.0g

Glucose 10.0g

Maltose 10.0g

Plant peptone 5.0g

pH 5.6 ± 0.2 at 25°C

Hi-Media

psi pressure–121°C Avoid overheating Pour into sterile Petri dishes or

leave in tubes

Use: For the cultivation and maintenance of a variety of fungi

Sabouraud Liquid Broth, Modified

See: Antibiotic Medium 13

Sabouraud Maltose Agar

Maltose 40.0g

Agar 15.0g

Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems and Oxoid Unipath

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Avoid overheating Pour into sterile Petri dishes or leave in tubes

Sabouraud Maltose Broth

Maltose 40.0g Neopeptone 10.0g

pH 5.6 ± 0.2 at 25°C

or flasks Autoclave for 15 min at 15 psi pressure–121°C Avoid over-heating

Use: For the cultivation of a variety of fungi

Sabouraud Maltose HiVeg Broth

Maltose 40.0g Plant peptone No 4 10.0g

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Avoid overheating

Sabouraud Medium, Emmons Modification

Glucose 20.0g Agar 15.0g Peptone 10.0g

pH 6.8–7.0 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 6.8–7.0 Gently heat and bring to boiling Distribute into tubes or flasks Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance ofArthroderma benhamiae, Arthroderma vanbreuseghemii, Aureobasidium pullulans, Epidermo-phyton floccosum, Microsporum canis, Sporothrix schenckii, Tricho-phyton mentagrophytes, and TrichoTricho-phyton rubrum.

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Saccharolytic Clostridia Medium 1533

Sabouraud Medium, Fluid

Glucose 20.0g

Peptamin 5.0g

pH 5.7 ± 0.2 at 25°C

Di-agnostic Systems and Oxoid Unipath

water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C Avoid

over-heating

Use: For the isolation and cultivation of yeasts and molds

Sabouraud Medium, Fluid, HiVeg

(Fluid Sabouraud HiVeg Medium)

Glucose 20.0g

Plant peptone 5.0g

pH 5.7 ± 0.2 at 25°C

Hi-Media

psi pressure–121°C

Use: For the cultivation and maintenance of a variety of fungi For the

sterility testing of pharmaceutical preparations for the presence of

molds and bacteria

Saccharococcus Agar

Agar 20.0g

Beef extract 5.0g

Sucrose 5.0g

Glucose 1.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Saccharococcus

thermo-philus.

Saccharolytic Clostridia Medium

Yeast extract 6.0g

Sodium thioglycolate 0.5g

Carbohydrate solution 100.0mL

Potassium phosphate (1M solution, pH 7.5) 30.0mL

MgSO4 (1M solution) 1.0mL

Solution M 0.5mL

FeSO4 solution 0.2mL

pH 7.0–7.2 at 25°C

Carbohydrate Solution:

Glucose or sucrose 20.0g

Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 100.0mL Mix

Solution M:

CaCl2 3.33g MnCl2·4H2O 1.98g

Na2MoO4·2H2O 1.21g CoCl2·6H2O 1.19g

Preparation of Solution M: Add components to distilled/deion-ized water and bring volume to 1.0L Mix thoroughly

FeSO 4 ·7H 2 O Solution:

FeSO4·7H2O 55.6g

H2SO4 (0.1M solution) 10.0mL

Preparation of FeSO 4 ·7H 2 O Solution: Add the FeSO4·7H2O to 10.0mL of H2SO4 solution Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 900.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 20 min

at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add sterile carbohydrate solution Mix thoroughly Aseptically distribute into ster-ile tubes

Use: For the cultivation of saccharolytic Clostridium species.

Saccharolytic Clostridia Medium

Sodium thioglycolate 1.0g

K2HPO4 0.8g

KH2PO4 0.2g MgSO4·7H2O 0.2g NaCl 0.2g

Na2MoO4·2H2O 0.025g Yeast extract 0.01g FeSO4·7H2O 0.01g MnSO4·4H2O 0.01g CaCl2 0.01g Carbohydrate solution 100.0mL Soil extract 10.0mL Trace elements solution 1.0mL

pH 7.2 ± 0.2 at 25°C

Carbohydrate Solution:

Glucose or sucrose 10.0g

Preparation of Carbohydrate Solution: Add glucose or sucrose

to distilled/deionized water and bring volume to 100.0mL Mix thor-oughly Filter sterilize

Soil Extract:

Garden soil, neutral 100.0g

Preparation of Soil Extract: Add garden soil to 100.0mL of tap water Gently heat and bring to 130°C for 60 min Cool to 45°C Filter through Whatman #1 filter paper Autoclave for 15 min at 15 psi pres-sure–121°C Cool to 45°–50°C

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1534 Saccharomyces Medium

Trace Elements Solution:

Na2B4O7·10H2O 0.05g

CoNO3·6H2O 0.05g

CdSO4·2H2O 0.05g

CuSO4·5H2O 0.05g

ZnSO4·7H2O 0.05g

MnSO4·H2O 0.05g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L Mix thoroughly

Preparation of Medium: Add components, except sodium

thiogly-colate and carbohydrate solution, to distilled/deionized water and bring

volume to 900.0mL Mix thoroughly Gently heat and bring to boiling

Add sodium thioglycolate Mix thoroughly Distribute 9.5mL into test

tubes that contain inverted Durham tubes Autoclave for 15 min at 15

psi pressure–121°C Cool to 45°–50°C Aseptically add 0.5mL of

ster-ile carbohydrate solution to each tube Mix thoroughly

Use: For the isolation of N2-fixing, saccharolytic Clostridium species.

Saccharomyces Medium

Glucose 20.0g

Peptone 20.0g

Agar 15.0g

Yeast extract 10.0g

Yeast nitrogen base without amino acids 6.7g

(NH4)2SO4 5.0g

Proline 4.0mg

Preparation of Medium: Add glucose, yeast nitrogen base without

amino acids, and proline to distilled/deionized water and bring volume

to 200.0mL Mix thoroughly Filter sterilize Add peptone, yeast

ex-tract, (NH4)2SO4, and agar to distilled/deionized water and bring

vol-ume to 800.0mL Mix thoroughly Gently heat and bring to boiling

Autoclave for 15 min at 15 psi pressure–121°C Cool to 50°C

Asepti-cally combine the two sterile solutions Mix thoroughly Pour into

ster-ile Petri dishes or aseptically distribute into sterster-ile tubes

Use: For the cultivation and maintenance of Saccharomyces

cerevi-siae.

Saccharomyces rouxii Medium

Glucose 400.0g

Agar 20.0g

Peptone 20.0g

Yeast extract 10.0g

Use: For the cultivation of Zygosaccharomyces rouxii

Use: For the cultivation of Monodictys austrina.

Sakazakii DHL Agar

Agar 15.0g

Casein enzymic hydrolysate 10.0g

Meat peptone 10.0g Lactose 10.0g Sucrose 10.0g Meat extract 3.0g

Na2S2O3 2.0g Sodium deoxycholate 1.5g Sodium citrate 1.0g Ammonium iron (III) citrate 1.0g

L-Cysteine·HCl·H2O 0.2g Neutral Red 0.03g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available from HiMedia

Use: For the detection and isolation of pathogenic Enterobacteriaceae from all types of specimens

Saline Czapek Agar (SCZA)

Sucrose 30.0g NaCl 25.0g Agar 15.0g NaNO3 3.0g

K2HPO4 1.0g KCl 0.5g MgSO4·7H20 0.5g FeSO4·7H2O 0.01g

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except sucrose, to dis-tilled/deionized water and bring volume to 900.0mL Mix thoroughly Distribute into tubes or flasks In a separate flask, add sucrose to dis-tilled/deionized water and bring volume to 100.0mL Mix thoroughly Autoclave both solutions separately for 15 min at 15 psi pressure– 121°C Cool to 50°C Combine the sterile solutions Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

Salinibacter ruber Agar

(DSMZ Medium 936)

NaCl 195.0g MgSO4·7H2O 49.5g MgCl2·6H2O 34.6g Agar 20.0g KCl 5.0g CaCl2·2H2O 1.25g Yeast extract 1.0g NaBr 0.625g NaHCO3 0.25g

pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Salinibacter ruber.

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