Handbook of Microbiological Media, Fourth Edition part 140 pot

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.. 5.0–10.0g Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized wate

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Phenol Red Broth Base with Plant Extract No 1 1385

Adonitol 5.0g

Beef extract 1.0g

Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available from HiMedia

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L Mix thoroughly Distribute into tubes

with inverted Durham tubes Autoclave for 15 min at 15 psi pressure–

121°C

Use: For the detection of adonitol fermenting bacteria

Phenol Red Agar

Composition per liter:

Agar 15.0g

Pancreatic digest of casein 10.0g

NaCl 5.0g

Phenol Red 0.018g

Carbohydrate solution 20.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD

Di-agnostic Systems

Carbohydrate Solution:

Compositionper 20.0mL:

Carbohydrate 5.0–10.0g

Preparation of Carbohydrate Solution: Add carbohydrate to

distilled/deionized water and bring volume to 20.0mL Mix

thorough-ly Filter sterilize

Preparation of Medium: Add components, except carbohydrate

solution, to distilled/deionized water and bring volume to 980.0mL

Mix thoroughly Adjust pH to 7.4 if necessary Autoclave for 15 min at

15 psi pressure–121°C Cool to 45°–50°C Aseptically add 20.0mL of

sterile carbohydrate solution Pour into sterile Petri dishes or distribute

into sterile tubes Allow tubes to cool in a slanted position

Use: For the determination of fermentation reactions Bacteria that can

ferment the added carbohydrate turn the medium yellow

Phenol Red Agar

Agar 15.0g

Proteose peptone No 3 10.0g

NaCl 5.0g

Beef extract 1.0g

Phenol Red 0.025g

pH 7.4 ± 0.2 at 25°C

Di-agnostic Systems

15 psi pressure–121°C Cool to 45°–50°C Aseptically add 20.0mL of sterile carbohydrate solution Pour into sterile Petri dishes or distribute into sterile tubes Allow tubes to cool in a slanted position

Use: For the determination of fermentation reactions Bacteria that can ferment the added carbohydrate turn the medium yellow

Phenol Red Arabinose Broth

Compositionper liter:

Proteose peptone 10.0g NaCl 5.0g Arabinose 5.0g Beef extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into tubes with inverted Durham tubes Autoclave for 15 min at 15 psi pressure– 121°C

Use: For the detection of arabinose-fermenting bacteria

Phenol Red Broth

Pancreatic digest of casein 10.0g NaCl 5.0g Phenol Red 0.018g Carbohydrate solution 20.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems

Composition per 20.0mL:

Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 20.0mL Mix

Preparation of Medium: Add components, except carbohydrate so-lution, to distilled/deionized water and bring volume to 980.0mL Mix thoroughly Adjust pH to 7.4 if necessary Distribute into tubes contain-ing an inverted Durham tube Fill each tube with 9.8mL of medium Au-toclave for 15 min at 13 psi pressure–118°C Cool to 45°–50°C Aseptically add 0.2mL of sterile carbohydrate solution to each tube

Use: For the determination of fermentation reactions in the differenti-ation of microorganisms Fermentdifferenti-ation is determined by the produc-tion of acid—broth turns yellow—and formaproduc-tion of gas—bubble trapped in Durham tube

Phenol Red Broth Base with Plant Extract No 1

Plant hydrolysate 10.0g NaCl 5.0g Plant extract No 1 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Source: This medium, without carbohydrate, is available as a pre-mixed powder from HiMedia

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1386 Phenol Red Carbohydrate Broth

sterile carbohydrate solution Aseptically distribute into sterile tubes or

flasks

Phenol Red Carbohydrate Broth

(BAM M121)

NaCl 5.0g

Beef extract 1.0g

Phenol Red solution 7.2mL

pH 7.4 ± 0.2 at 25°C

Di-agnostics

Phenol Red Solution:

Phenol Red 0.025g

Preparation of Phenol Red Solution: Add Phenol Red to

dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly

Preparation of Carbohydrate Solution: Add carbohydrate (5.0g

dulcitol, 10.0g lactose, or 10.0g sucrose) to distilled/deionized water

and bring volume to 200.0mL Mix thoroughly Filter sterilize

so-lution, to distilled/deionized water and bring volume to 800.0mL Mix

thoroughly Adjust pH to 7.4 if necessary Distribute into tubes

contain-ing an inverted Durham tube Fill each tube with 8.0mL of medium

Au-toclave for 15 min at 13 psi pressure–118°C Cool to 45°–50°C

Aseptically add 2.0mL of sterile carbohydrate solution to each tube

Use: For the determination of fermentation reactions in the

differenti-ation of microorganisms, such as Salmonella spp Fermentdifferenti-ation is

de-termined by the production of acid—broth turns yellow—and

formation of gas—bubble trapped in Durham tube

Phenol Red Carbohydrate Broth

with Sodium Chloride (BAM M121)

NaCl 30.0g

Beef extract 1.0g

Carbohydrate solution 200.0mL Phenol Red solution 7.2mL

pH 7.4 ± 0.2 at 25°C

Source: This medium, without added NaCl, is available as a premixed powder from BD Diagnostics

Phenol Red 0.025g

Preparation of Phenol Red Solution: Add Phenol Red to dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly

Preparation of Carbohydrate Solution: Add carbohydrate (5.0g dulcitol, 10.0g lactose, or 10.0g sucrose) to distilled/deionized water and bring volume to 200.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except carbohydrate so-lution, to distilled/deionized water and bring volume to 800.0mL Mix thoroughly Adjust pH to 7.4 if necessary Distribute into tubes contain-ing an inverted Durham tube Fill each tube with 8.0mL of medium Au-toclave for 15 min at 13 psi pressure–118°C Cool to 45°–50°C Aseptically add 2.0mL of sterile carbohydrate solution to each tube

Use: For the determination of fermentation reactions in the

differenti-ation of halophilic Vibrio spp Fermentdifferenti-ation is determined by the

pro-duction of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube

Phenol Red Dextrose Broth

Proteose peptone 10.0g NaCl 5.0g Glucose 5.0g Beef extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Use: For glucose fermentation studies of microorganisms

Phenol Red Dulcitol HiVeg Broth

Plant peptone No 3 10.0g Dulcitol 5.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

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Phenol Red Glucose HiVeg Agar 1387

Use: For the determination of the ability of a microorganism to ferment

dulcitol Fermentation is determined by the production of acid—medium

turns yellow

Phenol Red Galactose Broth

Proteose peptone 10.0g

NaCl 5.0g

Galactose 5.0g

Beef extract 1.0g

Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

121°C

Use: For galactose fermentation studies of microorganisms

Phenol Red Glucose Broth

Glucose 5.0g

NaCl 5.0g

Phenol Red 0.018g

pH 7.3 ± 0.2 at 25°C

Di-agnostic Systems

water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.3 if

necessary Distribute into tubes containing an inverted Durham tube

Fill each tube with 10.0mL of medium Autoclave for 15 min at 13 psi

pressure–118°C

Use: For determination of the ability of a microorganism to ferment

glucose Fermentation is determined by the production of acid—broth

turns yellow—and formation of gas—bubble trapped in Durham tube

Phenol Red Glucose Broth

(BAM M122)

Glucose 5.0g

NaCl 5.0g

Beef extract 1.0g

Phenol Red solution 7.2mL

pH 7.4 ± 0.2 at 25°C

Di-agnostics

Phenol Red 0.025g

Preparation of Phenol Red Solution: Add Phenol Red to

dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly

Fill each tube with 10.0mL of medium Autoclave for 10 min at 13 psi pressure–118°C

Use: For determination of the ability of a microorganism to ferment glucose Fermentation is determined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube

Phenol Red Glucose Broth, HiVeg

Plant peptone No 3 10.0g Glucose 5.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.3 if necessary Distribute into tubes containing an inverted Durham tube Fill each tube with 10.0mL of medium Autoclave for 15 min at 13 psi pressure–118°C

Use: For determination of the ability of a microorganism to ferment glucose Fermentation is determined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube

Phenol Red Glucose Broth with Sodium Chloride

(BAM M122)

NaCl 30.0g Proteose peptone No 3 10.0g Glucose 5.0g Beef extract 1.0g Phenol Red solution 7.2mL

pH 7.4 ± 0.2 at 25°C

Source: This medium, without added NaCl, is available as a premixed powder from BD Diagnostics

Phenol Red 0.025g

Preparation of Phenol Red Solution: Add Phenol Red to dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly

Use: For determination of the ability of halophilic Vibrio spp to

fer-ment glucose Ferfer-mentation is determined by the production of acid— broth turns yellow—and formation of gas—bubble trapped in Durham tube

Phenol Red Glucose HiVeg Agar

Agar 15.0g Plant peptone No 3 10.0g Glucose 10.0g NaCl 5.0g

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1388 Phenol Red HiVeg Agar Base with Carbohydrate

Plant extract 1.0g

Phenol Red 0.025g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from

Hi-Media

water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 13

psi pressure–118°C Pour into sterile Petri dishes or leave in tubes

Al-low tubes to cool in a slanted position

glucose Fermentation is determined by the production of acid—medium

turns yellow

Phenol Red HiVeg Agar Base with Carbohydrate

Agar 15.0g

Plant peptone No 3 10.0g

NaCl 5.0g

Plant extract 1.0g

Phenol Red 0.025g

pH 7.4 ± 0.2 at 25°C

Source: This medium, without carbohydrate, is available as a

pre-mixed powder from HiMedia

sterile carbohydrate solution Pour into sterile Petri dishes or distribute

into sterile tubes Allow tubes to cool in a slanted position

Phenol Red HiVeg Broth Base

NaCl 5.0g

Plant extract 1.0g

Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Source: This medium, without carbohydrate, is available as a

pre-mixed powder from HiMedia

Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 980.0mL Mix thoroughly Adjust pH to 7.4 if necessary Autoclave for 15 min at

15 psi pressure–121°C Cool to 45°–50°C Aseptically add 20.0mL of sterile carbohydrate solution Aseptically distribute into sterile tubes or flasks

Use: For the determination of fermentation reactions Bacteria that can ferment the added carbohydrate turn the medium yellow

Phenol Red Inositol Broth

Proteose peptone 10.0g NaCl 5.0g Inositol 5.0g Beef extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Use: For inositol fermentation studies of microorganisms

Phenol Red Lactose Agar

Agar 15.0g Lactose 10.0g Proteose peptone No 3 10.0g NaCl 5.0g Beef extract 1.0g Phenol Red 25.0mg

pH 7.4 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 13 psi pressure–118°C Pour into sterile Petri dishes or leave in tubes Al-low tubes to cool in a slanted position

Use: For the determination of the ability of a microorganism to ferment lactose Fermentation is determined by the production of acid—medium turns yellow

Phenol Red Lactose Broth

Pancreatic digest of casein 10.0g Lactose 5.0g NaCl 5.0g Phenol Red 0.018g

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.3 if necessary Distribute into tubes containing an inverted Durham tube

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Phenol Red Maltose HiVeg Broth 1389

pressure–118°C

lactose Fermentation is determined by the production of acid—broth

NaCl 5.0g

Lactose 5.0g

Beef extract 1.0g

Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

121°C

Use: For the detection of lactose-fermenting bacteria

Peptic digest of animal tissue 10.0g

Lactose 10.0g

NaCl 5.0g

Beef extract 1.0g

Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

121°C

Use: For lactose fermentation studies in accordance with the ISO

Committee under the specification ISO 9308-1:1990

Phenol Red Lactose HiVeg Agar

Agar 15.0g

Lactose 10.0g

NaCl 5.0g

Plant extract 1.0g

Phenol Red 0.025g

pH 7.4 ± 0.2 at 25°C

Hi-Media

Al-low tubes to cool in a slanted position

lactose Fermentation is determined by the production of acid—medium

turns yellow

Phenol Red Lactose HiVeg Broth

Plant peptone No 3 10.0g Lactose 5.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Use: For the determination of the ability of a microorganism to ferment lactose Fermentation is determined by the production of acid—medium turns yellow

Phenol Red Maltose Broth

Proteose peptone 10.0g NaCl 5.0g Maltose 5.0g Beef extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Use: For maltose fermentation studies of microorganisms

Phenol Red Maltose HiVeg Agar

Agar 15.0g Plant peptone No 3 10.0g Maltose 10.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.025g

pH 7.4 ± 0.2 at 25°C

Use: For the determination of the ability of a microorganism to ferment maltose Fermentation is determined by the production of acid—medium turns yellow

Phenol Red Maltose HiVeg Broth

Plant peptone No 3 10.0g Maltose 5.0g

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1390 Phenol Red Mannitol Agar

NaCl 5.0g

Plant extract 1.0g

Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Hi-Media

psi pressure–118°C

maltose Fermentation is determined by the production of acid—medium

turns yellow

Phenol Red Mannitol Agar

Agar 15.0g

Mannitol 10.0g

NaCl 5.0g

Beef extract 1.0g

Phenol Red 25.0mg

pH 7.4 ± 0.2 at 25°C

Di-agnostic Systems

to boiling Distribute into tubes or flasks Autoclave for 15 min at 13 psi

pressure–118°C Pour into sterile Petri dishes or leave in tubes Allow

tubes to cool in a slanted position

mannitol Fermentation is determined by the production of acid—

medium turns yellow

Phenol Red Mannitol Broth

D-Mannitol 5.0g

NaCl 5.0g

Phenol Red 0.018g

pH 7.3 ± 0.2 at 25°C

Di-agnostic Systems

pressure–118°C

mannitol Fermentation is determined by the production of acid—broth

Phenol Red Mannitol HiVeg Agar

Agar 15.0g

Mannitol 10.0g

NaCl 5.0g Plant extract 1.0g Phenol Red 0.025g

pH 7.4 ± 0.2 at 25°C

Use: For the determination of the ability of a microorganism to ferment mannitol Fermentation is determined by the production of acid—medium turns yellow

Phenol Red Mannitol HiVeg Broth

Plant peptone No 3 10.0g Mannitol 5.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Use: For the determination of the ability of a microorganism to ferment mannitol Fermentation is determined by the production of acid—medium turns yellow

Phenol Red Raffinose Broth

Proteose peptone 10.0g NaCl 5.0g Raffinose 5.0g Beef extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Use: For raffinose fermentation studies of microorganisms

Phenol Red Rhamnose Broth

Proteose peptone 10.0g NaCl 5.0g Rhamnose 5.0g Beef extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

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Phenol Red Sucrose HiVeg Broth 1391

121°C

Use: For rhamnose fermentation studies of microorganisms

Phenol Red Salicin Broth

NaCl 5.0g

Salicin 5.0g

Beef extract 1.0g

Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

121°C

Use: For salicin fermentation studies of microorganisms

Phenol Red Sorbitol Broth

NaCl 5.0g

Sorbitol 5.0g

Beef extract 1.0g

Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

121°C

Use: For sorbitol fermentation studies of microorganisms

Phenol Red Starch Broth

NaCl 5.0g

Starch 5.0g

Beef extract 1.0g

Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

121°C

Use: For starch fermentation studies of microorganisms

Phenol Red Sucrose Broth

NaCl 5.0g

Sucrose 5.0g Phenol Red 0.018g

pH 7.3 ± 0.2 at 25°C

Use: For determination of the ability of a microorganism to ferment sucrose Fermentation is determined by the production of acid—broth turns yellow—and formation of gas—bubble trapped in Durham tube

Phenol Red Sucrose Broth

Proteose peptone 10.0g NaCl 5.0g Sucrose 5.0g Beef extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Use: For sucrose fermentation studies of microorganisms

Phenol Red Sucrose HiVeg Agar

Agar 15.0g Plant peptone No 3 10.0g Sucrose 10.0g NaCl 5.0g Plant extract 1.0g Phenol Red 0.025g

pH 7.4 ± 0.2 at 25°C

Use: For the determination of the ability of a microorganism to ferment sucrose Fermentation is determined by the production of acid—medium turns yellow

Phenol Red Sucrose HiVeg Broth

Plant peptone No 3 10.0g NaCl 5.0g Sucrose 5.0g Plant extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

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1392 Phenol Red Tartrate Agar

Hi-Media

psi pressure–118°C

sucrose Fermentation is determined by the production of acid—medium

turns yellow

Phenol Red Tartrate Agar

Agar 15.0g

Peptone 10.0g

Potassium tartrate 10.0g

NaCl 5.0g

Phenol Red 0.024g

pH 7.6 ± 0.2 at 25°C

Di-agnostic Systems

Preparation of Medium: Add components to cold

distilled/deion-ized water and bring volume to 1.0L Mix thoroughly Gently heat and

bring to boiling Distribute into tubes or flasks Autoclave for 15 min

at 13 psi pressure–118°C Pour into sterile Petri dishes or leave in

tubes Allow tubes to cool in an upright position

Use: For the differentiation of Gram-negative bacteria of the intestinal

groups, particularly members of the Salmonella (paratyphoid) group

based on their ability to ferment tartrate

Phenol Red Tartrate Broth

Potassium tartrate 10.0g

Agar 5.0g

NaCl 5.0g

Phenol Red 0.024g

pH 7.6 ± 0.2 at 25°C

or flasks Autoclave for 15 min at 15 psi pressure–121°C

Phenol Red Tartrate HiVeg Agar

Agar 15.0g

Plant peptone 10.0g

Sodium potassium tartrate 10.0g

NaCl 5.0g

Phenol Red 0.024g

pH 7.6 ± 0.2 at 25°C

Hi-Media

Preparation of Medium: Add components to cold

distilled/deion-ized water and bring volume to 1.0L Mix thoroughly Gently heat and

bring to boiling Distribute into tubes or flasks Autoclave for 15 min

at 13 psi pressure–118°C Pour into sterile Petri dishes or leave in

tubes Allow tubes to cool in an upright position

Phenol Red Trehalose Broth

Proteose peptone 10.0g NaCl 5.0g Trehalose 5.0g Beef extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Use: For trehalose fermentation studies of microorganisms

Phenol Red Xylose Broth

Proteose peptone 10.0g NaCl 5.0g Xylose 5.0g Beef extract 1.0g Phenol Red 0.018g

pH 7.4 ± 0.2 at 25°C

Use: For xylose fermentation studies of microorganisms

Phenolphthalein Phosphate HiVeg Agar

Agar 15.0g Plant peptone 5.0g NaCl 5.0g Plant extract 3.0g Sodium phenolphthalein phosphate 0.012g

pH 7.4 ± 0.2 at 25°C

Use: For the identification of phosphatase-positive Staphylococcus au-reus.

Phenylalanine Agar (Phenylalanine Deaminase Medium)

Agar 12.0g NaCl 5.0g Yeast extract 3.0g

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Phenylobacterium Agar 1393

DL-Phenylalanine 2.0g

Na2HPO4 1.0g

pH 7.3 ± 0.2 at 25°C

Di-agnostic Systems

water and bring volume to 1.0L Mix thoroughly Gently heat while

stirring and bring to boiling Distribute into tubes or flasks Autoclave

for 10 min at 15 psi pressure–121°C Pour into sterile Petri dishes or

leave in tubes

Use: For the differentiation of enteric Gram-negative bacilli on the

basis of their ability to produce phenylpyruvic acid from

phenylala-nine After appropriate incubation of bacteria, ferric chloride reagent is

added on the agar Formation of a green color in 1–5 min indicates the

production of phenylpyruvic acid

Phenylalanine Malonate Broth

Sodium malonate 3.0g

DL-Phenylalanine 2.0g

NaCl 2.0g

(NH4)2SO4 2.0g

Yeast extract 1.0g

K2HPO4 0.6g

KH2PO4 0.4g

Bromthymol Blue 0.025g

pH 7.3 ± 0.2 at 25°C

Di-agnostic Systems

Use: For the differentiation of Gram-negative enteric bacilli on the

basis of malonate utilization and formation of pyruvic acid from

phe-nylalanine

Phenylethanol Agar

Agar 15.0g

Tryptose 10.0g

NaCl 5.0g

Beef extract 3.0g

Phenylethanol 2.5g

pH 7.3 ± 0.2 at 25°C

Di-agnostic Systems

Use: For the isolation of staphylococci and streptococci from

speci-mens containing a mixed flora

Phenylethanol Blood Agar

Agar 15.0g

Tryptose 10.0g

NaCl 5.0g Beef extract 3.0g Phenylethanol 2.5g Blood, defibrinated 50.0mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except blood, to dis-tilled/deionized water and bring volume to 950.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at 13 psi pres-sure–118°C Cool to 45°–50°C Aseptically add sterile defibrinated blood Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the isolation of staphylococci and streptococci from speci-mens containing a mixed flora

Phenylethyl Alcohol Agar

See: Phenethyl Alcohol Agar

Phenylethyl Alcohol HiVeg Agar

Agar 15.0g Plant hydrolysate 15.0g Papaic digest of soybean meal 5.0g NaCl 5.0g Phenylethyl alcohol 2.5g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the isolation of staphylococci and streptococci from speci-mens containing a mixed flora

Phenylketonuria Test Agar

See: PKU Test Agar

Phenylobacterium Agar

Agar 20.0g Chloridazon herbicide 2.0g Peptone 1.0g Yeast extract 1.0g

Na2HPO4·12H2O 0.7g (NH4)2HPO4 0.7g

KH2PO4 0.3g

NH4H2PO4 0.3g MgSO4·7H2O 0.25g (NH4)2SO4 0.1g CaCl2·6H2O 0.05g

H3BO3 0.5mg MnSO4·4H2O 0.4mg ZnSO4·7H2O 0.4mg FeCl3·6H2O 0.2mg (NH4)6Mo7O24·4H2O 0.2mg Biotin 0.1mg KJ 0.1mg CuSO4·5H2O 0.04mg Vitamin B12 0.03mg

pH 6.8–7.0 at 25°C

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1394 Phenylobacterium Medium

Use: For the cultivation and maintenance of Phenylobacterium

immo-bile.

Phenylobacterium Medium

Agar 15.0g

Antipyrin 1.0g

Na2HPO4·12H2O 0.7g

(NH4)2HPO4 0.7g

KH2PO4 0.3g

NH4H2PO4 0.3g

MgSO4·7H2O 0.25g

FeCl3·6H2O 0.2g

(NH4)2SO4 0.1g

CaCl2·6H2O 50.0mg

H3BO3 0.5mg

MnSO4·4H2O 0.4mg

ZnSO4·7H2O 0.4mg

(NH4)2MoO4 0.2mg

Biotin 0.1mg

CuSO4·5H2O 0.1mg

KI 0.1mg

Vitamin B12 30.0μg

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation of Phenylobacterium immobile.

Phenylobacterium Medium

Antipyrine 1.0g

Na2HPO4·12H2O 0.7g

(NH4)2HPO4 0.7g

KH2PO4 0.3g

NH4H2PO4 0.3g

(NH4)2SO4 0.1g

MgSO4·7H2O 0.25g

CaCl2·6H2O 0.05g

H3BO3 0.5mg

MnSO4·4H2O 0.4mg

ZnSO4·7H2O 0.4mg

FeCl3·6H2O 0.2mg

(NH4)2MoO4 0.2mg

Biotin 0.1mg

KI 0.1mg

CuSO4·5H2O 0.04mg

Vitamin B12 0.03mg

Use: For the cultivation and maintenance of Phenylobacterium immobile.

Phosphate Mineral Salts Medium with Octane

(NH4)2HPO4 10.0g

K2HPO4 5.0g

Na2SO4 0.5g Octane 10.0mL

Preparation of Medium: Add components, except octane, to tap water and bring volume to 990.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Prior to inoculation, filter sterilize oc-tane Aseptically add sterile octane to sterile medium Aseptically dis-tribute into sterile tubes or flasks

Use: For the cultivation and maintenance of Pseudomonas oleo-vorans.

Photobacterium Agar (DSMZ Medium 32)

Seawater aquarium salt 33.0g Agar 20.0g Tris 6.0g Yeast extract 5.0g Tryptone 5.0g

NH4Cl 5.0g Glycerol 3.0g CaCO3 1.0g

pH 7.2–7.5 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.2– 7.5 Gently heat and bring to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Vibrio fischeri.

Photobacterium Agar

Seawater aquarium salt 33.0g Agar 20.0g Tris(hydroxymethyl)aminomethane 6.0g

NH4Cl 5.0g Pancreatic digest of casein 5.0g Yeast extract 5.0g Glycerol 3.0g CaCO3 1.0g

pH 7.2 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Acetobacter pasteur-ianus.

Photobacterium Broth

(DSMZ Medium 608)

NaCl 30.0g Na-glycerol phosphate 23.5g Tryptone 5.0g

KH2PO4 3.0g

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