Handbook of Microbiological Media, Fourth Edition part 132 doc

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.

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Nutrient Agar No 2 Diluted 1/100 1305

Plant peptone 5.0g

Plant extract 3.0g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from

Hi-Media

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15

psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of a wide variety of

micro-organisms

Nutrient Agar 1.5%, HiVeg with Ascitic Fluid

Compositionper liter:

Agar 15.0g

NaCl 8.0g

Plant peptone 5.0g

Plant extract 3.0g

Ascitic fluid 250.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium, without ascitic fluid, is available as a premixed

powder from HiMedia

Preparation of Medium: Add components, except ascitic fluid, to

distilled/deionized water and bring volume to 750.0mL Mix

thorough-ly Autoclave for 15 min at 15 psi pressure–121°C Cool to less than

37°C Aseptically add sterile ascitic fluid If desired, 0.5g of thallium

acetate or 100,000U of penicillin may be added for a more selective

medium Mix thoroughly Aseptically distribute into sterile tubes or

flasks

Use: For the enrichment of pleuro-pneumonia-like organisms (PPLOs)

and Mycoplasma species from clinical specimens.

Nutrient Agar with Maltose

Agar 15.0g

Maltose 10.0g

Pancreatic digest of gelatin 5.0g

Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Source: Nutrient agar is available as a premixed powder from BD

Di-agnostic Systems

Use: For the cultivation and maintenance of Nocardia

corynebacteroi-des.

Nutrient Agar No 2 Compositionper liter:

Agar 20.0g

Meat extract 10.0g

Peptone 10.0g

NaCl 5.0g

pH 7.0 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of a wide variety of bacteria

Nutrient Agar No 2, Diluted 1/10 (DSMZ Medium 952) Compositionper liter:

Agar 20.0g Peptone 1.0g Meat extract 1.0g NaCl 0.5g

pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.1 Gently heat and bring to boiling Distribute into tubes or flasks Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance of Agromonas oligotrophica.

Nutrient Agar No 2, Diluted 1/10 Compositionper liter:

Agar 20.0g Meat extract 1.0g Peptone 1.0g NaCl 0.5g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring

Use: For the cultivation and maintenance of Agromonas

oligotroph-ica and Kurthia gibsonii.

Nutrient Agar No 2 Diluted 1/100 (DSMZ Medium 952) Compositionper liter:

Agar 20.0g Peptone 0.1g Meat extract 0.1g NaCl 50.0mg

pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.1 Gently heat and bring to boiling Distribute into tubes or flasks Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance of Bacillus pumilus.

Nutrient Agar No 2 Diluted 1/100 Compositionper liter:

Agar 20.0g Meat extract 0.1g Peptone 0.1g NaCl 50.0mg

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 7.0

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1306 Nutrient Agar No 2, HiVeg

Gently heat and bring to boiling Distribute into tubes or flasks

Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

oligotroph-ica.

Nutrient Agar No 2, HiVeg

Agar 15.0g

Plant extract 10.0g

Plant peptone 10.0g

NaCl 5.0g

pH 7.4 ± 0.2 at 25°C

Hi-Media

micro-organisms

Nutrient Agar Oxoid CM3 with Phosphate

(DSMZ Medium 605a) Compositionper liter:

Agar 15.0g

Peptone 5.0g

NaCl 5.0g

Na2HPO4·12H2O 2.39g

Yeast extract 2.0g

Lab-Lemco beef extract 1.0g

KH2PO4 0.45g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Devosia riboflavina,

Brevun-dimonas diminuta, and Herbaspirillum rubrisubalbicans.

Nutrient Agar with 1% Peptone, HiVeg

Agar 15.0g

Plant peptone 10.0g

Plant extract 5.0g

NaCl 5.0g

pH 7.4 ± 0.2 at 25°C

Hi-Media

micro-organisms

Nutrient Agar, pH 5.0 (Oxoid Nutrient Agar No 1, pH 5.0) Compositionper liter:

Agar 15.0g Peptone 5.0g NaCl 5.0g Yeast extract 2.0g Beef extract 1.0g

pH 5.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath

to boiling Adjust pH to 5.0 Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Pseudomonas phenazinium.

Nutrient Agar pH 6.0 Compositionper liter:

Agar 15.0g Peptone 5.0g Beef extract 3.0g

pH 6.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems

to boiling Adjust pH to 6.0 Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of microorganisms that prefer a slightly acid nutrient agar

Nutrient Agar pH 6.0 with 0.8% NaCl, HiVeg Compositionper liter:

Agar 15.0g NaCl 8.0g Plant peptone 5.0g Plant extract 3.0g

pH 6.0± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

Use: For the cultivation of microorganisms that prefer a slightly acid nutrient agar

Nutrient Agar pH 6.8 (LMG Medium 2) Compositionper liter:

Agar 15.0g Peptone 5.0g NaCl 5.0g

Na2HPO4·12 H2O 2.39g Yeast extract 2.0g

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Nutrient Agar pH 9.5 1307

KH2PO4 0.45g

pH 6.8 ± 0.2 at 25°C

water and bring volume to 1.0L Mix thoroughly Adjust pH to 6.8

Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–

121°C

Use: For the cultivation and maintenance of Acidovorax facilis,

Pseudo-monas geniculata, Arthrobacter siderocapsulatus, Delftia acidovorans,

Pseudomonas spp., and various other heterotrophic bacteria.

Nutrient Agar, pH 6.8, HiVeg

Agar 15.0g

Plant peptone 5.0g

Plant extract 3.0g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia

micro-organisms

Nutrient Agar pH 6.8, HiVeg with Blood

Agar 15.0g

Plant peptone 5.0g

Plant extract 3.0g

Sheep blood, defibrinated 50.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium, without blood, is available as a premixed

pow-der from HiMedia

Preparation of Medium: Add components, except sheep blood, to

thorough-ly Heat with frequent agitation and boil for 1 min to completely

dis-solve Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–

50°C Aseptically add 50.0mL of sterile, defibrinated sheep blood Mix

thoroughly and pour into sterile Petri dishes

Use: For the cultivation of streptococci and other fastidious

microor-ganisms

Nutrient Agar pH 6.8 with Horse Blood

(LMG Medium 234) Compositionper liter:

Agar 15.0g

Peptone 5.0g

NaCl 5.0g

Na2HPO4·12 H2O 2.39g

Yeast extract 2.0g

KH2PO4 0.45g

Horse blood, sterile defibrinated 50.0mL

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse blood, to distilled/deionized water and bring volume to 950.0mL Mix

thorough-ly Adjust pH to 6.8 Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 50.0mL sterile horse blood Mix thor-oughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Neisseria weaveri.

Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 8.0 ± 0.2 at 25°C Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Adjust pH to 8.0 Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes Allow tubes to cool in a slanted position

Use: For the cultivation and maintenance of Bacillus alcalophilus.

Nutrient Agar pH 9.0 (ATCC Medium 2029) Compositionper liter:

pH 9.0 ± 0.1 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Adjust pH to 9.0 Gently heat while stirring and bring to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of alkilophilic bacteria

Na2CO3 solution variable

pH 9.5–10.0 at 25°C

Na 2 CO 3 Solution:

Compositionper 100.0mL:

Na2CO3 5.0g

Preparation of Na 2 CO 3 Solution: Add Na2CO3 to distilled/de-ionized water and bring volume to 100.0mL Mix thoroughly Auto-clave for 15 min at 15 psi pressure–121°C Cool to 50°C

Preparation of Medium: Add components, except Na2CO3 solu-tion, to distilled/deionized water and bring volume to 1.0L Mix thor-oughly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Adjust pH to 9.5 with sterile

Na2CO3 solution Mix thoroughly Pour into sterile Petri dishes or dis-tribute into sterile tubes Allow tubes to cool in a slanted position

Use: For the cultivation and maintenance of Bacillus alcalophilus

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1308 Nutrient Agar pH 10.0

Nutrient Agar pH 10.0 (ATCC Medium 2030) Compositionper liter:

Agar 15.0g

Beef extract 3.0g

pH 10.0 ± 0.1 at 25°C

Source: This medium is available as a premixed powder from BD

Di-agnostic Systems

water and bring volume to 1.0L Mix thoroughly Adjust pH to 10.0

Gently heat while stirring and bring to boiling Distribute into tubes or

flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile

Petri dishes or leave in tubes

Use: For the cultivation of alkilophilic bacteria

Nutrient Agar with Cysteine

(ATCC Medium 812) Compositionper liter:

Agar 15.0g

Beef extract 3.0g

L-Cysteine 0.1g

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Use: For the cultivation and maintenance of Salmonella choleraesuis.

Nutrient Agar with Dihydrostreptomycin

Agar 15.0g

Beef extract 3.0g

Dihydrostreptomycin solution 10.0mL

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Dihydrostreptomycin Solution:

Dihydrostreptomycin 0.625g

Preparation of Dihydrostreptomycin Solution: Add

dihy-drostreptomycin to distilled/deionized water and bring volume to

10.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except

dihydrostrepto-mycin solution, to distilled/deionized water and bring volume to

990.0mL Mix thoroughly Gently heat and bring to boiling Autoclave

for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically

add sterile dihydrostreptomycin solution Mix thoroughly Pour into

sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Escherichia coli,

Micro-coccus luteus, Shigella species, and Vibrio cholerae.

Nutrient Agar with Erythromycin Compositionper liter:

Agar 15.0g Peptone 5.0g Beef extract 3.0g Erythromycin solution 10.0mL

pH 6.8 ± 0.2 at 25°C

Erythromycin Solution:

Erythromycin 0.6g

Preparation of Erythromycin Solution: Add erythromycin to distilled/deionized water and bring volume to 10.0mL Mix

thorough-ly Filter sterilize

Preparation of Medium: Add components, except erythromycin solution, to distilled/deionized water and bring volume to 990.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min

at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add sterile erythromycin solution Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Micrococcus luteus.

Nutrient Agar with Ethanolamine Compositionper liter:

Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Ethanolamine 2.0mL

pH 6.8 ± 0.2 at 25°C

Source: Nutrient agar is available as a premixed powder from BD Di-agnostic Systems

Use: For the cultivation and maintenance of Flavobacterium

tirreni-cum and Pseudomonas species.

Nutrient Agar with Ethylene Glycol Compositionper liter:

Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Ethylene glycol 2.0mL

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pseudomonas putida.

Nutrient Agar with Glucose Compositionper liter:

Agar 15.0g Pancreatic digest of gelatin 5.0g

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Nutrient Agar with 1% Methanol 1309

Beef extract 3.0g

Glucose 10.0g

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Use: For the cultivation and maintenance of Amycolata saturnea,

Arthrobacter species, Corynebacterium species, Curtobacterium

flaccum-faciens, Deinococcus radiodurans, Escherichia coli, Hafnia alvei,

Micro-coccus aurantiacus, Myxomicrobium multiplex, Nocardia petroleophila,

Nocardia species, Pseudomonas species, Rhodococcus rhodochrous,

Streptomyces piedadensis, and Xanthomonas species.

Nutrient Agar with Horse Serum

Agar 15.0g

Peptone 5.0g

NaCl 5.0g

Yeast extract 2.0g

Horse serum 100.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to

distilled/deionized water and bring volume to 1.0L Mix thoroughly

Gently heat and bring to boiling Autoclave for 15 min at 15 psi

pres-sure–121°C Cool medium to 45–50°C Aseptically add 100.0mL of

sterile horse serum Mix thoroughly Pour into sterile Petri dishes or

distribute into sterile tubes

Use: For the cultivation of fastidious bacteria

Nutrient Agar with Horse Serum

Agar 15.0g

Beef extract 3.0g

Horse serum 100.0mL

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Preparation of Medium: Add components, except horse serum, to

thorough-ly Gently heat and bring to boiling Autoclave for 15 min at 15 psi

pressure–121°C Cool to 45°–50°C Aseptically add sterile horse

se-rum Mix thoroughly Pour into sterile Petri dishes or distribute into

sterile tubes

Use: For the cultivation and maintenance of Alysiella filiformis and

Simonsiella crassa.

Nutrient Agar with 10% Horse Serum

(DSMZ Medium 302) Compositionper liter:

Agar 15.0g

Beef extract 3.0g Horse serum 100.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 900.0mL Mix

thorough-ly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool to 25°C Aseptically add 100.0mL filter steril-ized horse serum Mix thoroughly Pour into sterile Petri dishes or aseptically distrubute into tubes

Use: For the isolation and cultivation of Simonsiella crassa.

Nutrient Agar with Manganese

(NAMn) Compositionper liter:

Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Manganese sulfate solution 1.0mL

pH 6.8 ± 0.2 at 25°C

Manganese Sulfate Solution:

MnSO4·H2O 3.08g

Preparation of Manganese Sulfate Solution: Add MnSO4·H2O

to distilled/deionized water and bring volume to 100.0mL Mix thor-oughly

Use: For the cultivation of Bacillus species from canned foods For enhanced spore production by Bacillus species.

Nutrient Agar with Manganese Sulfate

(ATCC Medium 1859) Compositionper liter:

Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Manganese sulfate solution 1.0mL

pH 6.8 ± 0.2 at 25°C

Manganese Sulfate Solution:

MnSO4·H2O 0.5g

Preparation of Manganese Sulfate Solution: Add MnSO4·H2O

to distilled/deionized water and bring volume to 100.0mL Mix thor-oughly

Use: For the isolation and cultivation of Paenibacillus chondroitinus and Paenibacillus alginolyticus.

Nutrient Agar with 1% Methanol (ATCC Medium 620) Compositionper liter:

Agar 15.0g Pancreatic digest of gelatin 5.0g

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1310 Nutrient Agar with 2% Methanol

Beef extract 3.0g

Methanol 10.0mL

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Preparation of Medium: Filter sterilize methanol Add

compo-nents, except methanol, to distilled/deionized water and bring volume

to 990.0mL Mix thoroughly Gently heat and bring to boiling

Auto-clave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C

Asepti-cally add sterile methanol Mix thoroughly Pour into sterile Petri

dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Bacillus species,

Methyl-omonas clara, and PseudMethyl-omonas methanolica.

Nutrient Agar with 2% Methanol

(ATCC Medium 628) Compositionper liter:

Agar 15.0g

Beef extract 3.0g

Methanol 20.0mL

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Preparation of Medium: Filter sterilize methanol Add

compo-nents, except methanol, to distilled/deionized water and bring volume

to 980.0mL Mix thoroughly Gently heat and bring to boiling

Auto-clave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C

Asepti-cally add sterile methanol Mix thoroughly Pour into sterile Petri

dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Pseudomonas species.

Nutrient Agar with 0.5% Sodium Chloride

Agar 15.0g

NaCl 5.0g

Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Use: For the cultivation and maintenance of Agrobacterium

tumefa-ciens, Escherichia coli, Pseudomonas aeruginosa, Salmonella

choler-aesuis, Shigella dysenteriae, Shigella flexneri, Vibrio species, and

Yersinia species.

Nutrient Agar with 0.5% Sodium Chloride,

pH 9.5-10.0 (LMG Medium 253) Compositionper liter:

Agar 15.0g

NaCl 10.0g

Peptone 5.0g

Yeast extract 2.0g Lab-Lemco beef extract 1.0g

pH 9.5–10.0

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°-50°C Adjust pH to 9.5-10.0 with ster-ile Na2CO3 solution Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Bacillus spp.

Nutrient Agar with 1.5% Sodium Chloride Compositionper liter:

NaCl 15.0g Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Photobacterium

leiog-nathi, Pseudomonas fluorescens, and Vibrio natriegens

Nutrient Agar with 2% Sodium Chloride Compositionper liter:

NaCl 20.0g Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Vibrio alginolyticus,

Vibrio bivalvii, Vibrio mediterranei, Vibrio natriegens, Vibrio ordali, Vibrio orientalis, Vibrio parahaemolyticus, Vibrio pelagigius, and Vibrio vulnificus.

Nutrient Agar with 3% Sodium Chloride

NaCl 35.0g Agar 15.0g Peptone 5.0g Yeast extract 2.0g Lab-Lemco beef extract 1.0g

pH 7.4 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Adjust pH to 9.5–10.0 with sterile Na2CO3 solution Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Bacillus cirroflagellosus.

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Nutrient Agar with Nalidixic Acid 1311

NaCl 30.0g

Agar 15.0g

Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Use: For the cultivation and maintenance of Bacillus species,

Altero-monas nigrifaciens, Halococcus species, Planococcus citreus,

Pseudomonas beijerinckii, Staphylococcus species, Streptococcus

pyogenes, and Vibrio species.

Nutrient Agar with 5% Sodium Chloride, pH 9.5–10.0

NaCl 55.0g

Agar 15.0g

Peptone 5.0g

Yeast extract 2.0g

pH 9.5–10.0

psi pressure–121°C Cool to 45°–50°C Adjust pH to 9.5–10.0 with

sterile Na2CO3 solution Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Bacillus haloalkaliphilus.

NaCl 100.0g

Agar 15.0g

Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Use: For the cultivation and maintenance of Paracoccus

halodenitrif-icans and Micrococcus species.

and Maltose Compositionper liter:

NaCl 100.0g

Agar 15.0g

Maltose 10.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Paracoccus

halodenitrif-icans and Micrococcus species.

Nutrient Agar with 0.5% Sodium Chloride

and Sodium Citrate Compositionper liter:

Agar 15.0g NaCl 5.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Sodium citrate 2.94g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Escherichia coli.

Nutrient Agar with Nalidixic Acid (ATCC Medium 2089) Compositionper liter:

Agar 15.0g Pancreatic digest of gelatin 5.0g NaCl 5.0g Beef extract 3.0g Nalidixic acid solution 10.0mL

pH 6.8 ± 0.2 at 25°C

Nalidixic Acid Solution:

Nalidixic acid 0.1g

Preparation of Nalidixic Acid Solution: Add nalidixic acid to distilled/deionized water and bring volume to 10.0mL Mix

thorough-ly Filter sterilize

Preparation of Medium: Add components, except nalidixic acid solution, to distilled/deionized water and bring volume to 990.0mL Mix thoroughly Gently heat while stirring and bring to boiling Auto-clave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Asepti-cally add sterile nalidixic acid solution Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation of nalidixic acid-resistant bacteria

Nutrient Agar with Phosphate

See: Nutrient Agar, Buffered

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1312 Nutrient Agar with Phytone™

Nutrient Agar with Phytone™

Agar 15.0g

Phytone™ 10.0g

Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

water and bring volume to 1.0L Mix thoroughly Gently heat while

stirring and bring to boiling Distribute into tubes or flasks Autoclave

for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or

leave in tubes

Use: For the cultivation of a wide variety of bacteria

Nutrient Agar with Potato Starch

Agar 15.0g

Potato starch 10.0g

Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

water and bring volume to 1.0L Mix thoroughly Gently heat while

stirring and bring to boiling Distribute into tubes or flasks Autoclave

for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or

leave in tubes

Use: For the cultivation and maintenance of Bacillus polymyxa and

Bacillus subtilis.

Nutrient Agar with Soil Extract

Agar 15.0g

Beef extract 3.0g

Soil extract 250.0mL

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Soil Extract:

African Violet soil 115.5g

Na2CO3 0.3g

Preparation of Soil Extract: Add components to tap water and

bring volume to 300.0mL Autoclave for 60 min at 15 psi pressure–

121°C Filter through Whatman filter paper

psi pressure–121°C Pour into sterile Petri dishes

Use: For the cultivation and maintenance of Auerobacterium species,

Bacillus species, and Saccharomonospora viridis.

Nutrient Agar with Streptomycin

Agar 15.0g

Peptone 5.0g

Beef extract 3.0g Streptomycin solution 10.0mL

pH 6.8 ± 0.2 at 25°C

Streptomycin Solution:

Streptomycin 0.02g

Preparation of Streptomycin Solution: Add streptomycin to dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except streptomycin solution, to distilled/deionized water and bring volume to 990.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min

at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add sterile streptomycin solution Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Corynebacterium

glutam-icum and Corynebacterium herculis.

Nutrient Agar with Sucrose Compositionper liter:

Sucrose 20.0g Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pseudomonas species.

Nutrient Agar with 2% Sucrose (ATCC Medium 1297) Compositionper liter:

Sucrose 20.0 g Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation of osmophilic bacteria

Nutrient Agar with Tetracycline Compositionper liter:

Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Tetracycline solution 10.0mL

pH 6.8 ± 0.2 at 25°C

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Nutrient Broth 1313

Tetracycline Solution:

Tetracycline 0.025g

Preparation of Tetracycline Solution: Add tetracycline to

dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly

Filter sterilize

Preparation of Medium: Add components, except tetracycline

so-lution, to distilled/deionized water and bring volume to 990.0mL Mix

thoroughly Gently heat and bring to boiling Autoclave for 15 min at

15 psi pressure–121°C Cool to 45°–50°C Aseptically add sterile

tet-racycline solution Mix thoroughly Pour into sterile Petri dishes or

dis-tribute into sterile tubes

Use: For the cultivation and maintenance of Salmonella choleraesuis.

Nutrient Agar with Uracil

Agar 15.0g

Beef extract 3.0g

Uracil 0.01g

pH 6.8 ± 0.2 at 25°C

Di-agnostic Systems

Use: For the cultivation and maintenance of Escherichia coli.

Nutrient Agar with 5% Urea

Agar 15.0g

Beef extract 3.0g

Urea solution 100.0mL

pH 6.8 ± 0.2 at 25°C

Urea Solution:

Urea 50.0g

Preparation of Urea Solution: Add urea to distilled/deionized

wa-ter and bring volume to 100.0mL Mix thoroughly Filwa-ter swa-terilize

Preparation of Medium: Add components, except urea solution, to

thorough-ly Gently heat and bring to boiling Autoclave for 15 min at 15 psi

pressure–121°C Cool to 45°–50°C Aseptically add 100.0mL of sterile

urea solution Mix thoroughly Pour into sterile Petri dishes or

asepti-cally distribute into sterile tubes

Use: For the cultivation and maintenance of Aspergillus species.

Nutrient Agar with Ureum

Ureum 20.0g

Agar 15.0g

Peptone 5.0g

NaCl 5.0g Yeast extract 2.0g Lab-Lemco beef extract 1.0g

pH 7.4 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Sporosarcina pasteurii.

Nutrient Agar with V-8™ Juice Compositionper liter:

Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g V-8™ juice 200.0mL

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pseudomonas tolaasii.

Nutrient Agar with Yeast Extract Compositionper liter:

Yeast extract 20.0g Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Bacillus anthracis and

Comamonas testosteroni.

Nutrient Broth Compositionper liter:

Peptone 5.0g NaCl 5.0g Yeast extract 2.0g Beef extract 1.0g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of a wide variety of nonfastidious microorgan-isms

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1314 Nutrient Broth

Nutrient Broth (DSMZ Medium 948) Compositionper liter:

Peptone 5.0g

NaCl 5.0g

Yeast extract 2.0g

pH 7.2 ± 0.2 at 25°C

water and bring volume to 1.0L Mix thoroughly Distribute into tubes

Use: For the cultivation of Psychrobacter urativorans.

Nutrient Broth Compositionper liter:

Beef extract 3.0g

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD

Di-agnostic Systems

water and bring volume to 1.0L Mix thoroughly Distribute into tubes

Use: For the cultivation of a wide variety of nonfastidious

microorgan-isms

Nutrient Broth with Bovine Serum

NaCl 10.0g

Beef extract 3.0g

Bovine serum 100.0mL

pH 6.9 ± 0.2 at 25°C

Preparation of Medium: Add components, except bovine serum,

to distilled/deionized water and bring volume to 900.0mL Mix

thor-oughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–

50°C Aseptically add sterile bovine serum Mix thoroughly

Aseptical-ly distribute into sterile tubes or flasks

Use: For the cultivation and maintenance of Pseudomonas

anguil-liseptica.

Nutrient Broth, Diluted 1:100

Nutrient Broth:

Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Preparation of Nutrient Broth: Add components to

distilled/de-ionized water and bring volume to 1.0L Mix thoroughly

Preparation of Medium: Add 10.0mL of nutrient broth to distilled/

deionized water and bring volume to 1.0L Mix thoroughly Distribute

into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

oligotroph-ica.

Nutrient Broth Glycerol Medium Compositionper liter:

Glycerol 100.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 7.2–7.4 ± 0.2 at 25°C

Use: For the cultivation of Pseudomonas species.

Nutrient Broth with Manganese Sulfate Compositionper liter:

MnSO4 5.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 6.9 ± 0.2 at 25°C

Use: For the cultivation of Bacillus chondroitinus and Bacillus

algi-nolyticus.

Nutrient Broth with Rifampicin Compositionper liter:

Peptone 5.0g NaCl 5.0g Yeast extract 2.0g Beef extract 1.0g Rifampicin solution 10.0mL

pH 7.4 ± 0.2 at 25°C

Rifampicin Solution:

Rifampicin 10.0mg

Preparation of Rifampicin Solution: Add rifampicin to distilled/ deionized water and bring volume to 10.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except rifampicin solu-tion, to distilled/deionized water and bring volume to 990.0mL Mix thoroughly Adjust pH to 7.4 Autoclave for 15 min at 15 psi pressure– 121°C Cool to room temperature Aseptically add 10.0mL of sterile ri-fampicin solution Mix thoroughly Aseptically distribute into sterile tubes or flasks

Use: For the cultivation of Agrobacterium tumefaciens

Nutrient Broth with 6% Sodium Chloride Compositionper liter:

NaCl 60.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation of organisms in water, sewage, feces, and

other materials For the cultivation and maintenance of Paracoccus

halodenitrificans.

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