Handbook of Microbiological Media, Fourth Edition part 124 ppt

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.

Trang 1

Motility Test and Maintenance Medium 1225

Use: For the detection of motility in Gram-negative nonfermentative

bacteria

Motility Nitrate Medium, Buffered

Composition per liter:

Peptone 5.0g

Galactose 5.0g

Agar 3.0g

Beef extract 3.0g

Na2HPO4 2.5g

KNO3 1.0g

Glycerin 5.0mL

pH 7.3 ± 0.1 at 25°C

Preparation of Medium: Add components, except agar, to

dis-tilled/deionized water and bring volume to 1.0L Mix thoroughly

Gen-tly heat until dissolved Add agar GenGen-tly heat until boiling Distribute

into tubes in 11.0mL volumes Autoclave for 15 min at 15 psi pressure–

121°C If not used within 4 hr, heat tubes to 100°C for 10 min

Use: For the cultivation and observation of the motility of Clostridium

perfringens.

Motility Sulfide Medium

Composition per liter:

Gelatin 80.0g

Proteose peptone 10.0g

NaCl 5.0g

Agar 4.0g

Beef extract 3.0g

Sodium citrate 2.0g

L-Cystine 0.2g

Ferrous ammonium citrate 0.2g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD

Di-agnostic Systems

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L Mix thoroughly Gently heat while

stirring and bring to boiling Distribute into tubes in 4–5.0mL volumes

Autoclave for 15 min at 10 psi pressure–116°C

Use: For the determination of bacterial motility and the ability of

bac-teria to produce H2S from L-cystine For the differentiation of

Gram-negative bacteria of the Enterobacteriaceae

Motility Test HiVeg Medium

Plant hydrolysate No 1 10.0g

Agar 5.0g

NaCl 5.0g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from

Hi-Media

water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes in 4.0mL volumes Autoclave for 15

min at 15 psi pressure–121°C

Use: For the detection of bacterial motility

Motility Test HiVeg Medium (Edwards and Ewing HiVeg Medium)

Plant peptone 10.0g NaCl 5.0g Agar 4.0g Plant extract 3.0g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes in 4.0mL volumes Autoclave for 15 min at 15 psi pressure–121°C

Use: For testing motility of enteric bacteria

Motility Test HiVeg Medium with Triphenyltetrazolium Chloride (Edwards and Ewing HiVeg Medium)

Plant peptone 10.0g NaCl 5.0g Agar 4.0g Plant extract 3.0g Triphenyltetrazolium chloride solution 5.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium, without triphenyltetrazolium chloride solution,

is available as a premixed powder from HiMedia

Triphenyltetrazolium Choride Solution:

Composition per 5.0mL:

Triphenyltetrazolium chloride 0.1g

Preparation of Triphenyltetrazolium Choride Solution: Add triphenyltetrazolium chloride to distilled/deionized water and bring volume to 5.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except triphenyltetra-zolium chloride solution, to distilled/deionized water and bring volume

to 1.0L.Mix thoroughly Gently heat and bring to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Cool

to 45°–50°C Aseptically add 5.0mL triphenyltetrazolium chloride so-lution Mix thoroughly Aseptically distribute into tubes

Motility Test and Maintenance Medium

Peptone 10.0g NaCl 5.0g Agar 4.0g Beef extract 3.0g 2,3,5-Triphenyltetrazolium chloride 0.05g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into screw-capped tubes in 8.0mL volumes Autoclave for 15 min at 15 psi pres-sure–121°C

Use: For the cultivation, maintenance, and observation of the motility

of Listeria monocytogenes.

Trang 2

1226 Motility Test and Maintenance Medium

Agar 9.0g

Tryptose 8.0g

NaCl 5.0g

Pancreatic digest of gelatin 2.5g

Beef extract 1.5g

pH 7.2 ± 0.1 at 25°C

to boiling Distribute into tubes in 7.0mL volumes Autoclave for 15

min at 15 psi pressure–121°C Cool to 45°–50°C Pass the cooled tubes

into an anaerobic chamber containing 85% N2 + 10% H2 + 5% CO2

Use: For the cultivation, maintenance, and observation of the motility

in a variety of anaerobic bacteria

Peptone 10.0g

NaCl 5.0g

Agar 4.0g

Beef extract 3.0g

pH 7.4 ± 0.1 at 25°C

wa-ter and bring volume to 1.0L Mix thoroughly Distribute into screw-capped

tubes in 8.0mL volumes Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation, maintenance, and observation of motility in

members of the Enterobacteriaceae

Motility Test and Maintenance Medium, Gilardi

Pancreatic digest of casein 10.0g

NaCl 5.0g

Agar 3.0g

Yeast extract 3.0g

pH 7.2 ± 0.1 at 25°C

water and bring volume to 1.0L Mix thoroughly Distribute into

screw-capped tubes in 3.5mL volumes Autoclave for 15 min at 15 psi

pres-sure–121°C

Use: For the cultivation, maintenance, and observation of motility in

nonfermenting Gram–negative bacteria

Motility Test and Maintenance Medium, Tatum

Tryptose 8.0g

NaCl 5.0g

Agar 4.0g

Beef extract 1.5g

pH 6.9 ± 0.2 at 25°C

water and bring volume to 1.0L Mix thoroughly Distribute into

screw-capped tubes in 8.0mL volumes Autoclave for 15 min at 15 psi

pres-sure–121°C

Use: For the cultivation, maintenance, and observation of motility in nonfermenting Gram–negative bacteria

Motility Test Medium

Pancreatic digest of gelatin 10.0g NaCl 5.0g Agar 4.0g Beef extract 3.0g Triphenyltetrazolium chloride solution 5.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-agnostic Systems

2,3,5-Triphenyltetrazolium Chloride Solution:

2,3,5-Triphenyltetrazolium chloride 0.1g

Preparation of 2,3,5-Triphenyltetrazolium Chloride Solu-tion: Add 2,3,5-triphenyltetrazolium chloride to distilled/deionized water and bring volume to 10.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components to distilled/deionized water and bring volume to 995.0mL Mix thoroughly Gently heat while stirring and bring to boiling Autoclave for 15 min at 15 psi pres-sure–121°C Cool to 45°–50°C Aseptically add 5.0mL of sterile 2,3,5-triphenyltetrazolium chloride solution Mix thoroughly Aseptically distribute into sterile tubes

Use: For detection of the motility of Gram-negative enteric bacteria

Motility Test Medium

Tryptose 10.0g NaCl 5.0g Agar 5.0g

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat while stirring and bring to boiling Distribute into tubes in 4–5.0mL volumes Autoclave for 15 min at 15 psi pressure–121°C Cool tubes quickly in

an upright position

Use: For the determination of bacterial motility

Motility Test Medium, Semisolid

Peptone 10.0g NaCl 5.0g Agar 4.0g Beef extract 3.0g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat while stirring and bring to boiling Distribute into screw-capped tubes in 8.0mL or 20.0mL volumes Autoclave for 15 min at 15 psi pressure– 121°C Pour into sterile Petri dishes in 20.0mL volumes or leave in tubes

Trang 3

MP 5 Medium 1227

Use: For the cultivation and observation of motility in a variety of

bac-teria, especially Salmonella species.

Motility Test Medium, Semisolid

with Sodium Chloride (BAM M103)

NaCl 25.0g

Peptone 10.0g

Agar 4.0g

Beef extract 3.0g

pH 7.4 ± 0.2 at 25°C

stirring and bring to boiling Distribute into screw-capped tubes in

8.0mL volumes Autoclave for 15 min at 15 psi pressure–121°C Leave

in tubes

Use: For the cultivation and observation of motility in a variety of

bac-teria, especially Salmonella spp and Listeria spp For Listeria spp keep

tubes tightly screw capped and sealed with parafilm For Salmonella spp.

use Petri plates prepared on the same day as use

Use: For the cultivation of yeasts and fungi

MOX Agar

See: Magnesium Oxalate Agar

See: Oxford Agar, Modified

MOX HiVeg Agar

Agar 15.0g

Plant hydrolysate 15.0g

Papaic digest of soybean meal 5.0g

NaCl 5.0g

MnCl2 4.067g

Sodium oxalate 2.68g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from

Hi-Media

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15

psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of Yersinia enterocolitica from foods.

MP Agar

Agar 15.0g

Sodium acetate 0.1g

Basal medium 1.0L

Sodium sulfide solution 3.0mL

pH 7.0–7.5 at 25°C

Basal Medium:

CaSO4·2H2O (saturated solution) 20.0mL

NH4Cl (4% solution) 5.0mL

Trace elements solution 5.0mL

MgSO4·7H2O (1% solution) 1.0mL

K2HPO4 (1% solution) 1.0mL

Preparation of Basal Medium: Add components to distilled/de-ionized water and bring volume to 1.0L Mix thoroughly

Trace Elements Solution:

Ferrous EDTA solution 20.0mL ZnSO4·7H2O (0.1% solution) 10.0mL MnSO4·4H2O (0.02% solution) 10.0mL CuSO4·5H2O (0.00005% solution) 10.0mL

H3BO3 (0.1% solution) 10.0mL Co(NO3)2 or CoCl2·6H2O (0.01% solution) 10.0mL

Na2MoO4·2H2O (0.01% solution) 10.0mL

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly

Ferrous EDTA Solution:

FeSO4·7H2O 7.0g EDTA 2.0g HCl, concentrated 1.0mL

Preparation of Ferrous EDTA Solution: Add components to distilled/deionized water and bring volume to 100.0mL Mix thorough-ly

Sodium Sulfide Solution:

Na2S·9H20 1.0g

Preparation of Sodium Sulfide Solution: Add Na2S·9H20 to dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Prepare freshly

Preparation of Medium: Add sodium acetate and agar to 1.0L of basal medium Mix thoroughly Adjust pH to 7.0–7.5 Gently heat and bring to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool

to 45°–50°C Aseptically add 3.0mL of sterile sodium sulfide solution immediately prior to dispensing Mix thoroughly Pour into sterile Petri dishes or distribute into sterile screw-capped tubes

Use: For the isolation and cultivation of Beggiatoa species and myx-otrophic strains of Thiothrix species from water and environmental

sources

MP 5 Medium (Mineral Pectin 5 Medium)

Agar solution 500.0mL Basal medium 250.0mL Mineral solution 250.0mL

pH 5.0–6.0 at 25°C

Agar Solution:

Agar 15.0g

Preparation of Agar Solution: Add agar to distilled/deionized water and bring volume to 500.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool

to 45°–50°C

Basal Medium:

Na2HPO4 6.0g Pectin, citrus or apple 5.0g

Trang 4

1228 MP 7 Medium

KH2PO4 4.0g

NH4SO4 2.0g

Yeast extract 1.0g

Preparation of Basal Medium: Add components to

distilled/de-ionized water and bring volume to 250.0mL Mix thoroughly Gently

heat and bring to boiling

Mineral Solution:

FeSO4 (0.1% solution) 1.0mL

CaCl2·2H2O (0.1% solution) 1.0mL

H3BO3 (0.001% solution) 1.0mL

MnSO4·H2O (0.001% solution) 1.0mL

ZnSO4·7H2O (0.007% solution 1.0mL

CuSO4·5H2O (0.005% solution) 1.0mL

MoO3 (0.001% solution) 1.0mL

Preparation of Mineral Solution: Add components to distilled/

deionized water and bring volume to 250.0mL Mix thoroughly

Preparation of Medium: Combine 250.0mL of basal medium and

250.0mL of mineral solution Mix thoroughly Adjust pH to 5.0–6.0 with

1N HCl Autoclave the basal medium-mineral solution and agar solution

separately for 15 min at 15 psi pressure–121°C Cool to 45°–50°C

Asep-tically combine the two sterile solutions Mix thoroughly Pour

immedi-ately into sterile Petri dishes to prevent hydrolysis of the agar

Use: For the cultivation of microorganisms that produce

polygalacta-nase

MP 7 Medium (Mineral Pectin 7 Medium)

Basal medium 500.0mL

Mineral solution 500.0mL

pH 7.2 ± 0.2 at 25°C

Basal Medium:

Agar 15.0g

Na2HPO4 6.0g

Pectin (citrus or apple) 5.0g

KH2PO4 4.0g

NH4SO4 2.0g

Yeast extract 1.0g

Preparation of Basal Medium: Add components to

distilled/de-ionized water and bring volume to 500.0mL Mix thoroughly Gently

heat and bring to boiling

Mineral Solution:

FeSO4 (0.1% solution) 1.0mL

CaCl2·2H2O (0.1% solution) 1.0mL

H3BO3 (0.001% solution) 1.0mL

MnSO4·H2O (0.001% solution) 1.0mL

ZnSO4·7H2O (0.007% solution 1.0mL

CuSO4·5H2O (0.005% solution 1.0mL

MoO3 (0.001% solution) 1.0mL

Preparation of Mineral Solution: Add components to distilled/

deionized water and bring volume to 500.0mL Mix thoroughly

Preparation of Medium: Combine 500.0mL of basal medium and 500.0mL of mineral solution Mix thoroughly Adjust pH to 7.2 Auto-clave for 15 min at 15 psi pressure–121°C Cool to 50°C Pour into sterile Petri dishes

Use: For the cultivation of microorganisms that produce pectate lyase

m-PA Agar

See: PA Agar

m-PA-C Agar

See: PA-C Agar

MPH Agar (Milk Protein Hydrolysate Agar)

Agar 15.0g Casein hydrolysate 9.0g Glucose 1.0g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat while stirring and bring to boiling Autoclave for 15 min at 15 psi pressure– 121°C Aseptically distribute into sterile tubes Cool to 43°–45°C be-fore using

Use: For use in the enumeration of bacteria in water and dairy prod-ucts

MPHM Medium

McCoy's 5A medium, modified 1.0L Peptone solution 250.0mL Hemin solution 25.0mL

Source: McCoy’s 5A medium, modified, is available from Gibco

McCoys 5A Medium, Modified:

Inorganic salt solution 400.0mL Other component solution 400.0mL Amino acid solution 100.0mL Vitamin solution 100.0mL

Inorganic Salt Solution:

NaCl 6.46g NaHCO3 2.2g NaH2PO4·H2O 0.58g KCl 0.4g CaCl2, anhydrous 0.1g MgSO4, anhydrous 97.67mg

Preparation of Inorganic Salt Solution: Add components to dis-tilled/deionized water and bring volume to 400.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 25°C

Other Component Solution:

D-Glucose 3.0g Peptone 0.6g

Trang 5

MPOB Medium 1229

Phenol Red 0.1g

Glutathione, reduced 0.5mg

Preparation of Other Component Solution: Add components

to distilled/deionized water and bring volume to 400.0mL Mix

thor-oughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 25°C

Amino Acid Solution:

L-Glutamine 219.2mg

L-Asparagine 45.0mg

L-Arginine·HCl 42.1mg

L-Isoleucine 39.3mg

L-Leucine 39.3mg

L-Lysine·HCl 36.5mg

L-Cysteine 31.5mg

L-Serine 26.3mg

L-Tyrosine·2Na·2H2O 26.2mg

L-Glutamic acid 22.1mg

L-Histidine·HCl·H2O 21.0mg

L-Aspartic acid 20.0mg

L-Hydroxyproline 19.7mg

L-Threonine 17.9mg

L-Valine 17.6mg

L-Proline 17.3mg

L-Phenylalanine 16.5mg

L-Methionine 15.0mg

L-Alanine 13.9mg

Glycine 7.5mg

L-Tryptophan 3.1mg

Preparation of Amino Acid Solution : Add components to

dis-tilled/deionized water and bring volume to 100.0mL Mix thoroughly

Filter sterilize

Vitamin Solution:

i-Inositol 36.0mg

Folic acid 10.0mg

Choline chloride 5.0mg

Vitamin B12 2.0mg

p-Aminobenzoic acid 1.0mg

Ascorbic acid 0.5mg

Niacinamide 0.5mg

Nicotinic acid 0.5mg

Pyridoxal·HCl 0.5mg

Pyridoxine·HCl 0.5mg

Biotin 0.2mg

D-Ca pantothenate 0.2mg

Riboflavin 0.2mg

Thiamine·HCl 0.2mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 100.0mL Mix thoroughly Filter

sterilize

Preparation of McCoys 5A Medium, Modified: Aseptically

combine 400.0mL of sterile inorganic salt solution, 400.0mL of sterile

other component solution, 100.0mL of sterile amino acid solution, and

100.0mL of sterile vitamin solution

Peptone Solution:

Proteose peptone No 3 7.5g

Preparation of Peptone Solution: Add components to distilled/ deionized water and bring volume to 300.0mL Mix thoroughly Auto-clave for 15 min at 15 psi pressure–121°C Cool to 25°C

Hemin Solution:

NaCl 0.8g

Na2HPO4 0.12g Hemin 0.05g KCl 0.04g MgCl2·6H2O 0.03g NaH2PO4·H2O 0.02g CaCl2 0.011g

Preparation of Hemin Solution: Add NaCl, KCl, NaH2PO4·H2O, and Na2HPO4 to distilled/deionized water and bring volume to 50.0mL Mix thoroughly Separately add the CaCl2 and MgCl2·6H2O

to distilled/deionized water and bring volume to 50.0mL Mix thor-oughly Combine the two solutions Mix thorthor-oughly Add 0.05g of

hemin Mix thoroughly Adjust to pH 9.0 with 10N NaOH to dissolve

the hemin Mix thoroughly Filter sterilize

Preparation of Medium: Aseptically combine 1.0L of sterile mod-ified McCoy’s medium 5A, 250.0mL of sterile peptone solution, and 25.0mL of sterile hemin solution Aseptically distribute into sterile screw-capped tubes or flasks

Use: For the cultivation of Trypanosoma theileri.

m-Plate Count Broth

See: Plate Count Broth

MPOB Medium

Disodium fumarate 3.2g

Na2HPO4·2H2O 0.53g

KH2PO4 0.41g NaCl 0.3g

NH4Cl 0.3g Yeast extract 0.2g CaCl2·2H2O 0.11g MgCl2·6H2O 0.10g Resazurin 0.5mg NaHCO3 solution 20.0mL

Na2S·9H2O solution 10.0mL Wolfe’s vitamin solution 10.0mL Selenite-tungstate solution 1.0mL Trace elements solution SL-10 1.0mL

pH 7.0–7.2 at 25°C

NaHCO 3 Solution:

NaHCO3 4.0g

Preparation of NaHCO 3 Solution: Add NaHCO3 to distilled/deion-ized water and bring volume to 20.0mL Mix thoroughly Sparge with 80%

N2 + 20% CO2 Autoclave for 15 min at 15 psi pressure–121°C

Na 2 S·9H 2 O Solution:

Na2S·9H2O 0.5g

Preparation of Na 2 S·9H 2 O Solution: Add Na2S·9H2O to distilled/ deionized water and bring volume to 10.0mL Mix thoroughly Sparge with 100% N2 Autoclave for 15 min at 15 psi pressure–121°C

Trang 6

1230 MPSS Broth

Wolfe’s Vitamin Solution:

Pyridoxine·HCl 10.0mg

p-Aminobenzoic acid 5.0mg

Lipoic acid 5.0mg

Nicotinic acid 5.0mg

Riboflavin 5.0mg

Thiamine·HCl 5.0mg

Calcium DL-pantothenate 5.0mg

Biotin 2.0mg

Folic acid 2.0mg

Vitamin B12 0.1mg

Preparation of Wolfe’s Vitamin Solution: Add components to

distilled/deionized water and bring volume to 1.0L Mix thoroughly

Filter sterilize

Selenite-tungstate Solution:

NaOH 0.5g

Na2WO4·2H2O 4.0mg

Na2SeO3·5H2O 3.0mg

Preparation of Selenite-tungstate Solution: Add components to

distilled/deionized water and bring volume to 1.0L Mix thoroughly

Sparge with 100% N2 Autoclave for 15 min at 15 psi pressure–121°C

Trace Elements Solution SL-10:

FeCl2·4H2O 1.5g

CoCl2·6H2O 190.0mg

MnCl2·4H2O 100.0mg

ZnCl2 70.0mg

Na2MoO4·2H2O 36.0mg

NiCl2·6H2O 24.0mg

H3BO3 6.0mg

CuCl2·2H2O 2.0mg

HCl (25% solution) 10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution Mix thoroughly Add distilled/deionized

water and bring volume to 1.0L Add remaining components Mix

thor-oughly Sparge with 100% N2 Autoclave for 15 min at 15 psi pressure–

121°C

Preparation of Medium: Prepare medium under 80% N2 + 20%

CO2 Add components, except NaHCO3 solution and Na2S·9H2O

solu-tion, to distilled/deionized water and bring volume to 970.0mL Mix

thor-oughly Sparge with 80% N2 + 20% CO2 Autoclave for 15 min at 15 psi

pressure–121°C Aseptically and anaerobically add 20.0mL of sterile

NaHCO3 solution and 10.0mL of sterile Na2S·9H2O solution Mix

thor-oughly Aseptically and anaerobically distribute into sterile tubes or flasks

After inoculation, bring culture bottles to 0.7 bar 80% N2 + 20% CO2

over-pressure

Use: For the cultivation of Syntrophobacter species.

m-Pseudomonas aeruginosa Agar

See: Pseudomonas aeruginosa Agar

MPSS Broth

Peptone 5.0g

MgSO4·7H2O 1.0g

(NH4)2SO4 1.0g

Succinic acid 1.0g

FeCl3·6H2O (0.2% solution) 1.0mL MnSO4·H2O (0.2% solution) 1.0mL

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of Spirillum volutans.

MPY Agar (Malt Peptone Yeast Extract Agar) (ATCC Medium 582)

Agar 15.0g Malt extract 5.0g Xylose 2.0g Fructose 2.0g Lactose 2.0g Peptone 1.0g Yeast extract 1.0g

pH 7.0 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Streptomyces naniwaen-sis.

MPY Agar (Maltose Peptone Yeast Extract Medium)

(ATCC Medium 518)

Agar 10.0g Maltose 2.0g Peptone 2.0g Yeast extract 1.0g

Potassium phosphate buffer (1M, pH 7.5) 10.0mL

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components, except potassium phosphate buffer, to distilled/deionized water and bring volume to 990.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Filter sterilize potassium phosphate buffer Aseptically add sterile potassium phos-phate buffer to sterile, cooled basal medium Distribute into sterile tubes or flasks

Use: For the cultivation and maintenance of Spirochaeta aurantia

MPY Broth (Maltose Peptone Yeast Extract Broth)

Maltose 2.0g Peptone 2.0g Yeast extract 1.0g Potassium phosphate buffer (1M, pH 7.5) 10.0mL

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components, except potassium phosphate buffer, to distilled/deionized water and bring volume to

Trang 7

MRS Agar with 0.5% Cysteine 1231

990.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–

121°C Filter sterilize potassium phosphate buffer Aseptically add

sterile potassium phosphate buffer to sterile cooled basal medium

Dis-tribute into sterile tubes or flasks

Use: For the cultivation and maintenance of Spirochaeta aurantia

MPYG Medium

See: Peptone Yeast Extract Glucose Medium, Modified

MRS Agar (DeMan, Rogosa, Sharpe Agar)

Glucose 20.0g

Peptone 10.0g

Agar 10.0g

Beef extract 8.0g

Sodium acetate·3H2O 5.0g

Yeast extract 4.0g

K2HPO4 2.0g

Triammonium citrate 2.0g

MgSO4·7H2O 0.2g

MnSO4·4H2O 0.05g

Sorbitan monooleate 1.0mL

pH 6.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath

psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of lactic acid bacteria

MRS Agar (DeMan, Rogosa, Sharpe Agar)

Glucose 18.5g

Agar 13.5g

Beef extract 8.0g

Yeast extract 4.0g

Sodium acetate 3.0g

K2HPO4 2.0g

Ammonium citrate 2.0g

Polysorbate 80 1.0g

MgSO4·7H2O 0.2g

MnSO4·4H2O 0.05g

pH 6.2 ± 0.2 at 25°C

Di-agnostic Systems

stirring and bring to boiling Distribute into tubes or flasks Autoclave

for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or

leave in tubes

Use: For the isolation and cultivation of Lactobacillus species from

clinical specimens, foods, and dairy products

MRS Agar with Cysteine (LMG Medium 118)

Glucose 20.0g Peptone 10.0g Agar 12.0g Beef extract 5.0g Sodium acetate·3H2O 5.0g Yeast extract 5.0g

KH2PO4 2.0g Diammonium hydrogen citrate 2.0g MgSO4·7H2O 0.2g MnSO4·4H2O 38.0mg

L-Cysteine solution 10.0mL Tween™ 80 1.0mL

pH 6.5 ± 0.2 at 25°C

L -Cysteine Solution:

Composition per 10.0mL:

L-Cysteine 0.5g

Preparation of L -Cysteine Solution: Add 0.1g of L-cysteine to distilled/deionized water and bring volume to 10.0mL Mix

thorough-ly Filter sterilize

Preparation of Medium: Add components, except L-cysteine solu-tion, to distilled/deionized water and bring volume to 990.0mL Mix thoroughly Adjust pH to 6.5 Gently heat and bring to boiling Distrib-ute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Cool to 50°C Aseptically add 10.0mL of sterile L-cysteine solution Mix thoroughly Aseptically pour into sterile Petri dishes or distribute into sterile tubes

Use: For the isolation and cultivation of Leuconostoc mesenteroides

and other lactic acid bacteria

MRS Agar with 0.5% Cysteine (LMG Medium 131)

Glucose 20.0g Agar 12.0g Peptone 10.0g Beef extract 5.0g Sodium acetate·3H2O 5.0g Yeast extract 5.0g

KH2PO4 2.0g Diammonium hydrogen citrate 2.0g MgSO4·7H2O 0.2g MnSO4·4H2O 38.0mg

L-Cysteine·HCl solution 100.0mL Tween™ 80 1.0mL

pH 6.5 ± 0.2 at 25°C

L -Cysteine·HCl Solution:

L-Cysteine·HCl 5.0g

Preparation of L -Cysteine·HCl Solution: Add 5.0g of L -cyste-ine·HCl to distilled/deionized water and bring volume to 10.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except L-cysteine·HCl solution, to distilled/deionized water and bring volume to 900.0mL Mix thoroughly Adjust pH to 6.5 Gently heat and bring to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–

Trang 8

1232 MRS Agar with Ethanol

121°C Cool to 50°C Aseptically add 100.0mL of sterile L

-cysteine·HCl solution Mix thoroughly Aseptically pour into sterile

Petri dishes or distribute into sterile tubes

Use: For the isolation and cultivation of Lactobacillus ruminis and

Lactobacillus vitulinus.

MRS Agar with Ethanol (LMG Medium 130)

Glucose 20.0g

Agar 12.0g

Peptone 10.0g

Beef extract 5.0g

Yeast extract 5.0g

KH2PO4 2.0g

Diammonium hydrogen citrate 2.0g

MgSO4·7H2O 0.2g

MnSO4·4H2O 38.0mg

Ethanol 100.0mL

Tween™ 80 1.0mL

pH 5.0± 0.2 at 25°C

Preparation of Medium: Add components, except ethanol, to

dis-tilled/deionized water and bring volume to 900.0mL Mix thoroughly

Autoclave for 15 min at 15 psi pressure–121°C Cool to 50°C

Asepti-cally add 100.0mL of sterile ethanol Mix thoroughly AseptiAsepti-cally

dis-tribute into sterile tubes or pour into sterile Petri dishes

Use: For the cultivation of Lactobacillus acetotolerans.

MRS Agar with Tomato Juice, pH 5.2

(LMG Medium 248)

Glucose 20.0g

Peptone 10.0g

Agar 12.0g

Beef extract 5.0g

Yeast extract 5.0g

KH2PO4 2.0g

MgSO4·7H2O 0.2g

MnSO4·4H2O 38.0mg

Tomato juice 100.0mL

Tween™ 80 1.0mL

pH 5.2 ± 0.2 at 25°C

water and bring volume to 1.0L Mix thoroughly Adjust pH to 5.2

Gently heat and bring to boiling Distribute into tubes or flasks

Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the isolation and cultivation of Lactobacillus kunkeei.

MRS Agar, Half Strength (LMG Medium 281)

Glucose 10.0g

Agar 6.0g

Peptone 5.0g Beef extract 2.5g Sodium acetate·3H2O 2.5g Yeast extract 2.5g

KH2PO4 1.0g Diammonium hydrogen citrate 1.0g MgSO4·7H2O 0.1g MnSO4·4H2O 19.0mg Tween™ 80 0.5mL

pH 6.5± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into tubes

Use: For the cultivation of Lactobacillus acidipiscis and Weissella thai-landensis.

MRS Broth

Glucose 20.0g Peptone 10.0g Beef extract 5.0g Sodium acetate·3H2O 5.0g Yeast extract 5.0g

K2HPO4 2.0g Diammonium hydrogen citrate 2.0g MgSO4·7H2O 0.2g MnSO4·4H2O 38.0mg Sorbitan monooleate 1.0mL

pH 6.2 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of Leuconostoc pseudomesenteroides, Leu-conostoc carnosum, and Lactobacillus crispatus.

MRS Broth (DeMan, Rogosa, Sharpe Broth)

K2HPO4 2.0g Triammonium citrate 2.0g MgSO4·7H2O 0.2g MnSO4·4H2O 0.05g Sorbitan monooleate 1.0mL

pH 6.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

Trang 9

MRS Broth with Ethanol 1233

MRS Broth (DeMan, Rogosa, Sharpe Broth)

Glucose 18.5g

Beef extract 8.0g

Yeast extract 4.0g

Sodium acetate 3.0g

K2HPO4 2.0g

Polysorbate 80 1.0g

MgSO4·7H2O 0.2g

MnSO4·4H2O 0.05g

pH 6.2 ± 0.2 at 25°C

Di-agnostic Systems

water and bring volume to 1.0L Mix thoroughly Distribute into tubes

Use: For the isolation and cultivation of Lactobacillus species from

clinical specimens, foods, and dairy products

(LMG Medium 166)

CaCO3 30.0g

Glucose 20.0g

Peptone 10.0g

Beef extract 5.0g

Yeast extract 5.0g

K2HPO4 2.0g

MgSO4·7H2O 0.2g

MnSO4·4H2O 38.0mg

pH 6.2 ± 0.2 at 25°C

psi pressure–121°C

Use: For the cultivation of Sporolactobacillus inulinus.

MRS Broth with Cysteine

Glucose 18.5g

Beef extract 8.0g

Yeast extract 4.0g

Sodium acetate 3.0g

K2HPO4 2.0g

Polysorbate 80 1.0g

MgSO4·7H2O 0.2g

MnSO4·4H2O 0.05g

L-Cysteine solution 0.5mL

pH 6.2 ± 0.2 at 25°C

L -Cysteine Solution:

L-Cysteine 0.1g

Preparation of L -Cysteine Solution: Add 0.1g of L-cysteine to distilled/deionized water and bring volume to 10.0mL Mix

thorough-ly Filter sterilize

Preparation of Medium: Add components, except L-cysteine solu-tion, to distilled/deionized water and bring volume to 999.5mL Mix thoroughly Adjust pH to 6.2 Autoclave for 15 min at 15 psi pressure– 121°C Aseptically add 0.5mL of sterile L-cysteine solution Mix thor-oughly Aseptically distribute into sterile tubes or flasks

Use: For the isolation and cultivation of Leuconostoc mesenteroides.

MRS Broth with Ethanol (LMG Medium 240)

KH2PO4 2.0g Diammonium hydrogen citrate 2.0g MgSO4·7H2O 0.2g MnSO4·4H2O 38.0mg Ethanol 100.0mL Tween™ 80 1.0mL

pH 5.0± 0.2 at 25°C

Preparation of Medium: Add components, except ethanol, to dis-tilled/deionized water and bring volume to 900.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 50°C Asepti-cally add 100.0mL of sterile ethanol Mix thoroughly AseptiAsepti-cally dis-tribute into sterile tubes or sterile flasks

MRS Broth with Ethanol

K2HPO4 2.0g Triammonium citrate 2.0g MgSO4·7H2O 0.2g MnSO4·4H2O 0.05g Sorbitan monooleate 1.0mL Ethanol (95% solution), filter sterilized 100.0mL

pH 5.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except ethanol, to dis-tilled/deionized water and bring volume to 900.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Aseptically add 100.0mL of sterile ethanol Mix thoroughly Aseptically distribute into sterile tubes or flasks

Trang 10

1234 MRS Chalk

MRS Chalk

CaCO3 30.0g

Glucose 20.0g

Beef extract 10.0g

Peptone 10.0g

Yeast extract 5.0g

K2HPO4 2.0g

MgSO4·7H2O 0.2g

MnSO4·4H2O 0.05g

Tween™ 80™ 1.0mL

pH 6.2 ± 0.4 at 25°C

wa-ter and bring volume to 1.0L Mix thoroughly Adjust pH to 6.2 Distribute

into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of Sporolactobacillus inulinus and other

Spor-olactobacillus species.

MRS with Fermented Wort

Solution A 800.0mL

Solution B 200.0mL

pH 6.2 ± 0.2 at 25°C

Solution A:

Glucose 20.0g

Peptone 10.0g

Agar 10.0g

Beef extract 8.0g

Yeast extract 4.0g

K2HPO4 2.0g

MgSO4·7H2O 0.2g

MnSO4·4H2O 0.05g

Preparation of Solution A: Add components to distilled/deionized

to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool to

50°–55°C

Solution B:

Malt extract 15.0g

Maltose 12.75g

Dextrin 2.75g

Glycerol 2.35g

K2HPO4 1.0g

NH4Cl 1.0g

Preparation of Solution B: Add components to distilled/deionized

to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool to

room temperature Inoculate with Saccharomyces cerevisiae to allow

wort to ferment Incubate for 24–48 hr Allow to cool to room

temper-ature Filter through Whatman filter paper to remove solids Filter

ster-ilize

Preparation of Medium: Aseptically combine 800.0mL of sterile solution A and 200.0mL of sterile solution B Mix thoroughly Asepti-cally distribute into sterile tubes or flasks

Use: For the cultivation of yeasts and fungi

MRS Fructose Medium

Beef extract 10.0g Fructose 10.0g Peptone 10.0g Yeast extract 5.0g

K2HPO4 2.0g Triammonium citrate 2.0g MgSO4·7H2O 0.2g MnSO4·4H2O 0.05g Tween™ 80 1.0mL

pH 6.2 ± 0.4 at 25°C

Preparation of Medium: Add components to distilled/deionized wa-ter and bring volume to 1.0L Mix thoroughly Adjust pH to 6.2 Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of Carnobacterium divergens.

MRS HiVeg Agar

(Lactobacillus MRS HiVeg Agar)

Glucose 20.0g Peptone 10.0g Agar 10.0g Plant extract 8.0g Sodium acetate·3H2O 5.0g Yeast extract 4.0g

K2HPO4 2.0g Triammonium citrate 2.0g MgSO4·7H2O 0.2g MnSO4·4H2O 0.05g

pH 6.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

MRS HiVeg Agar, Modified (Lactobacilli Heteroferm Screen HiVeg Agar)

Glucose 20.0g Agar 15.0g Plant peptone No 3 10.0g Sodium acetate 5.0g Yeast extract 5.0g 2-Phenylethyl alcohol 3.0g Ammonium citrate 2.0g

K2HPO4 2.0g MgSO4 0.1g MnSO4 0.05g

Định dạng
Số trang	10
Dung lượng	207,86 KB