Handbook of Microbiological Media, Fourth Edition part 86 pdf

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.

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Hippea Medium 845

HiCrome™ UTI Agar, Modified

(UTI Agar, Modified HiCrome™)

Compositionper liter:

Peptic digest of animal tissue 18.0g

Agar 15.0g

Chromogenic mixture 12.44g

Beef extract 4.0g

Casein enzymatic hydrolysate 4.0g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from

Hi-Media

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L Mix thoroughly Gently heat while

stirring and bring to boiling Mix to completely dissolve components

Do not autoclave Cool to 50°C Pour into sterile Petri dishes

Use: A chromogenic medium used for detecting and identifying

Enter-obacteria, Proteus species, and other bacteria involved in urinary tract

infections

HiFluoro™ Pseudomonas Agar Base

Pancreatic digest of gelatin 18.0g

Agar 15.0g

K2SO4 10.0g

Fluorogenic mixture 2.05g

MnCl2 1.4g

Cetrimide 0.3g

Glycerol 10.ml

pH 7.2 ± 0.2 at 25°C

Source: This medium, wihout glycerol, is available as a premixed

powder from HiMedia

water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15

psi pressure–121°C Pour into sterile Petri dishes

Use: For the selective isolation of Pseudomonas aeruginosa from

clin-ical and nonclinclin-ical specimens by the fluorogenic method

High Plate Count Agar Composition per liter:

Agar 15.0g

Casein, soluble 0.5g

K2HPO4 0.2g

MgSO4·7H2O 0.05g

FeCl3·4H2O 1.0mg

pH 7.2 ± 0.2 at 25°C

Source: This medium is available from HiMedia

psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For obtaining higher colony counts by the spread plate, pour plate

or membrane filter technique

High Salt Nutrient Agar Composition per liter:

NaCl 30.0g Agar 15.0g Peptic digest of animal tissue 5.0g Meat extract 5.0g

pH 8.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the isolation and cultivation of salt-tolerant Vibrio species.

High Salt Peptone Yeast Extract Agar Composition per liter:

NaCl 30.0g Agar 15.0g Peptic digest of animal tissue 10.0g Yeast extract 6.0g Meat extract 2.0g Glucose 2.0g

L-Cysteine·HCl·H2O 0.3g

pH 7.5 ± 0.2 at 25°C

Use: For the confirmation of Vibrio species.

Hippea Medium

(DSMZ Medium 854) Composition per 1010.0mL:

NaCl 25.0g Sulfur, powdered 10.0g Na-acetate 5.0g

MOPS [3-(N-morpholino) propane

sulfonic acid] 3.0g

Na2S·9H2O 0.5g

NH4Cl 0.33g CaCl2·2H2O 0.33g MgCl2·6H2O 0.33g KCl 0.33g

KH2PO4 0.33g Yeast extract 0.1g Resazurin 0.5mg Trace elements solution 10.0mL Vitamin solution 10.0mL

pH 6.1 ± 0.2 at 25°C

Trace Elements Solution:

MgSO4·7H2O 3.0g Nitrilotriacetic acid 1.5g NaCl 1.0g MnSO4·2H2O 0.5g CoSO4·7H2O 0.18g ZnSO4·7H2O 0.18g

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846 Hippurate Hydrolysis Broth

CaCl2·2H2O 0.1g

FeSO4·7H2O 0.1g

NiCl2·6H2O 0.025g

KAl(SO4)2·12H2O 0.02g

H3BO3 0.01g

Na2MoO4·4H2O 0.01g

CuSO4·5H2O 0.01g

Na2SeO3·5H2O 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water Dissolve by adjusting pH

to 6.5 with KOH Add remaining components Add distilled/deionized

water to 1.0L Mix thoroughly

Vitamin Solution:

Pyridoxine-HCl 10.0mg

Thiamine-HCl·2H2O 5.0mg

Riboflavin 5.0mg

Nicotinic acid 5.0mg

D-Ca-pantothenate 5.0mg

p-Aminobenzoic acid 5.0mg

Lipoic acid 5.0mg

Biotin 2.0mg

Folic acid 2.0mg

Vitamin B12 0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L Mix thoroughly Sparge

with 80% H2 + 20% CO2 Filter sterilize

Preparation of Medium: Add components, except vitamin

solu-tion, sulfur, and Na2S·9H2O, to distilled/deionized water and bring

vol-ume to 1.0L Mix thoroughly Adjust pH to 6.0 Sparge the medium

with 80% N2 + 20% CO2 gas mixture for 30 min Add Na2S·9H2O

Mix thoroughly Readjust the pH to 6.0–6.2 Dispense medium under

80% N2 + 20% CO2 gas mixture into anaerobe tubes or bottles

con-taining 100.0mg sulfur powder per 10mL medium Autoclave 20 min

at 110°C Prior to use inject 0.1mL sterile vitamin solution per 10.0mL

medium

Use: For the cultivation of Hippea maritima.

Hippurate Broth

See: Sodium Hippurate Broth

Hippurate Hydrolysis Broth

Composition per liter:

Heart infusion powder 10.0g

Sodium hippurate 10.0g

NaCl 5.0g

pH 7.4 ± 0.2 at 25°C

Use: For the detection of hippurate-hydrolyzing bacteria

Hirschia Medium

Pancreatic digest of casein 5.0g

HEPES 4.0g

Yeast extract 2.0g Artificial seawater 250.0mL Glucose solution 100.0mL

pH 7.4 ± 0.2 at 25°C

Glucose Solution:

Compositionper 100.0mL:

Glucose 0.25g

Preparation of Glucose Solution: Add glucose to distilled/deion-ized water and bring volume to 100.0mL Mix thoroughly Filter ster-ilize

Artificial Seawater:

NaCl 27.5g MgCl2·6H2O 5.38g MgSO4·7H2O 6.78g KCl 0.72g NaHCO3 0.2g CaCL2·2H2O 1.4g

Preparation of Artificial Seawater: Add components to distilled/ deionized water and bring volume to 1.0L Mix thoroughly

Preparation of Medium: Add components, except glucose solu-tion, to distilled/deionized water and bring volume to 1.0L Mix thor-oughly Adjust pH to 7.4 Autoclave for 15 min at 15 psi pressure– 121°C Cool to 25°C Aseptically add 100.0mL of sterile glucose solu-tion Mix thoroughly Aseptically distribute into sterile tubes or flasks

Use: For the cultivation of Hirschia baltica.

Hi-Sensitivity Test Agar Compositionper liter:

Casein enzymic hydrolysate 11.0g Agar 8.0g NaCl 3.0g Peptic digest of animal tissue 3.0g Glucose 2.0g

Na2HPO4 2.0g Sodium acetate 1.0g Starch, soluble 1.0g Magnesium glycerophosphate 0.2g Calcium gluconate 0.1g

L-Cystine hydrochloride 0.02g

L-Tryptophan 0.02g Adenine 0.01g Guanine 0.01g Uracil 0.01g Xanthine 0.01g Calcium pantothenate 3.0mg Biotin 3.0mg Nicotinamide 3.0mg Pyridoxine hydrochloride 3.0mg Manganese chloride 2.0mg ZnSO4 1.0mg CoSO4 1.0mg CuSO4 1.0mg Cyanocobalamin 1.0mg FeSO4 1.0mg Menadione 1.0mg Thiamine hydrochloride 0.04mg

pH 7.2 ± 0.2 at 25°C

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Hi-Sensitivity Test HiVeg Broth 847

Hi-Media

psi pressure–121°C Pour into sterile Petri dishes

Use: For antimicrobial susceptibility tests

Hi-Sensitivity Test Broth Compositionper liter:

Casein enzymic hydrolysate 11.0g

NaCl 3.0g

Glucose 2.0g

Na2HPO4 2.0g

Sodium acetate 1.0g

Starch, soluble 1.0g

Magnesium glycerophosphate 0.2g

Calcium gluconate 0.1g

L-Tryptophan 0.02g

Adenine 0.01g

Guanine 0.01g

Uracil 0.01g

Xanthine 0.01g

Calcium pantothenate 3.0mg

Biotin 3.0mg

Nicotinamide 3.0mg

Pyridoxine hydrochloride 3.0mg

Manganese chloride 2.0mg

ZnSO4 1.0mg

CoSO4 1.0mg

CuSO4 1.0mg

Cyanocobalamin 1.0mg

FeSO4 1.0mg

Menadione 1.0mg

Thiamine hydrochloride 0.04mg

pH 7.2 ± 0.2 at 25°C

Hi-Media

psi pressure–121°C

Use: For antimicrobial susceptibility testing

Hi-Sensitivity Test HiVeg Agar

Plant hydrolysate 11.0g

Agar 8.0g

NaCl 3.0g

Plant peptone 3.0g

Glucose 2.0g

Na2HPO4 2.0g

Sodium acetate 1.0g

Starch, soluble 1.0g

Magnesium glycerophosphate 0.2g

Calcium gluconate 0.1g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

Use: For antimicrobial susceptibility tests

Hi-Sensitivity Test HiVeg Broth Compositionper liter:

Plant hydrolysate 11.0g NaCl 3.0g Plant peptone 3.0g Glucose 2.0g

Na2HPO4 2.0g Sodium acetate 1.0g Starch, soluble 1.0g Magnesium glycerophosphate 0.2g Calcium gluconate 0.1g

pH 7.4 ± 0.2 at 25°C

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848 Hisitest Agar

Use: For antimicrobial susceptibility testing

Hisitest Agar Compositionper liter:

Casein enzymic hydrolysate 11.0g

Agar 8.0g

Buffer salt 3.3g

NaCl 3.0g

Glucose 2.0g

Starch 1.0g

Nucleoside basis 0.02g

Thiamine 0.02mg

pH 7.2 ± 0.2 at 25°C

Hi-Media

Use: For determination of antibiotic susceptibility of fastidious

micro-organisms

Histidans Agar Compositionper liter:

Agar 20.0g

Glucose 10.0g

Yeast extract 10.0g

Na2HPO4 0.95g

KH2PO4 0.91g

MgSO4·7H2O 0.5g

pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Streptomyces species.

Histoplasma capsulatum Agar

Agar 12.5g

Glucose 10.0g

Citric acid 10.0g

Potato starch 2.0g

α-Ketoglutaric acid 1.0g

L-Cystine·HCl·H2O 1.0g

Glutathione, reduced 0.5g

L-Asparagine 0.1g

L-Tryptophan 0.02g

Solution 1 250.0mL

Solution 3 40.0mL

Solution 2 10.0mL

Solution 4 10.0mL

Solution 8 10.0mL

Solution 5 1.0mL

Solution 6 0.1mL Solution 7 0.1mL

pH 6.5 ± 0.2 at 25°C

Solution 1:

KH2PO4 8.0g (NH4)2SO4 8.0g MgSO4·7H2O 0.86g CaCl2, anhydrous 0.08g ZnSO4·7H2O 0.05g

Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 500.0mL Mix thoroughly Bring volume to 1.0L with distilled/deionized water Store at 5°C

Solution 2:

FeSO4·7H2O 5.7g MnCl2·6H2O 0.8g NaMoO4·2H2O 0.15g HCl, concentrated 1.0mL

Preparation of Solution 2 : Add 1.0mL of concentrated HCl to

100.0mL of distilled water in a 1.0L volumetric flask Dissolve each component completely in the sequence given Bring volume to 1.0L with distilled/deionized water Store at 5°C Discard if red color or red precipitate appears

Solution 3:

Casein, acid-hydrolyzed, vitamin-free 10.0g

Preparation of Solution 3: Add casein to distilled/deionized water and bring volume to 100.0mL

Solution 4:

Calcium pantothenate 0.2g Inositol 0.2g Riboflavin 0.2g Thiamine·HCl 0.2g Nicotinamide 0.1g Biotin 0.01g

Preparation of Solution 4: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Store at −20°C

Solution 5:

Hemin 0.2g

NH4OH, concentrated 0.3mL

Preparation of Solution 5: Add hemin to approximately 30.0mL

of distilled/deionized water Add NH4OH Mix thoroughly until dis-solved Bring volume to 100.0mL with distilled/deionized water Store

at 5° C

Solution 6:

DL-Thioctic acid 0.01g Ethanol (95% solution) 10.0mL

Preparation of Solution 6: Add DL-thioctic acid to 10.0mL of eth-anol Mix thoroughly Store at −20°C

Solution 7:

Coenzyme A 0.01g

Na2S·5H2O (0.05% solution) 0.2mL

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Histoplasma capsulatum Agar 849

Preparation of Solution 7: Prepare Na2S·5H2O solution in freshly

boiled distilled/deionized water Add coenzyme A to 9.8mL of

dis-tilled/deionized water Mix thoroughly Add freshly prepared

Na2S·5H2O solution Mix thoroughly Store the solution at −20°C

Solution 8:

Oleic acid 0.1g

Preparation of Solution 8: Add oleic acid to 50.0mL of distilled/

deionized water Adjust pH to 9.0 with NaOH Gently heat until

dis-solved Bring volume to 100.0mL with distilled/deionized water Store

at 5°C

Preparation of Medium: Add components—except agar, potato

starch, and solution 8—to distilled/deionized water and bring volume

to 400.0mL Mix thoroughly Adjust pH to 6.5 with 20% KOH

solu-tion Filter sterilize In a separate flask, add potato starch to 50.0mL of

distilled/deionized water Add the starch solution to 450.0mL of

boil-ing distilled/deionized water Add 10.0mL of solution 8 and the agar

Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Cool

to 70°C Aseptically combine the two sterile solutions Pour into sterile

Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Histoplasma capsulatum

in the yeast phase For the cultivation of Histoplasma duboisii,

Blasto-myces dermatitidis, and Sprotrichum schenckii.

Histoplasma capsulatum Agar

Agar 15.0g

Glucose 10.0g

Potato starch 2.0g

L-Asparagine 0.1g

L-Tryptophan 0.02g

Solution 1 250.0mL

Solution 3 40.0mL

Solution 2 10.0mL

Solution 4 10.0mL

Solution 8 10.0mL

Solution 5 1.0mL

Solution 6 0.1mL

Solution 7 0.1mL

pH 6.5 ± 0.2 at 25°C

Solution 1:

KH2PO4 8.0g

(NH4)2SO4 8.0g

MgSO4·7H2O 0.86g

CaCl2, anhydrous 0.08g

Preparation of Solution 1: Add components to distilled/deionized

water and bring volume to 500.0mL Mix thoroughly Bring volume to

1.0L with distilled/deionized water Store at 5°C

Solution 2:

FeSO4·7H2O 5.7g

MnCl2·6H2O 0.8g

NaMoO4·2H2O 0.15g

HCl, concentrated 1.0mL

Preparation of Solution 2: Add the 1.0mL of concentrated HCl to 100.0mL of distilled water in a 1.0L volumetric flask Dissolve each component completely in the sequence given Bring volume to 1.0L with distilled/deionized water Store at 5°C Discard if red color or red precipitate appears

Solution 3:

Preparation of Solution 3: Add casein to distilled/deionized water and bring volume to 100.0mL Do not use enzymatically digested ca-sein

Solution 4:

Calcium pantothenate 0.2g Inositol 0.2g Riboflavin 0.2g Thiamine·HCl 0.2g Nicotinamide 0.1g Biotin 0.01g

Solution 5:

Hemin 0.2g

at 5°C

Solution 6:

Preparation of Solution 6: Add DL-thioctic acid to 10.0mL of ethanol Mix thoroughly Store solution at −20°C

Solution 7:

Coenzyme A 0.01g

Preparation of Solution 7: Prepare Na2S·5H2O solution in freshly boiled distilled/deionized water Add coenzyme A to 9.8mL of dis-tilled/deionized water Mix thoroughly Add freshly prepared

Solution 8:

Oleic acid 0.1g

Preparation of Solution 8: Add oleic acid to 50.0mL of distilled/ deionized water Adjust pH to 9.0 with NaOH Gently heat until dis-solved Bring volume to 100.0mL with distilled/deionized water Store

at 5°C

Preparation of Medium: Add components—except agar, potato starch, and solution 8—to distilled/deionized water and bring volume

to 400.0mL Mix thoroughly Adjust pH to 6.5 with 20% KOH solu-tion Filter sterilize In a separate flask, add potato starch to 50.0mL of distilled/deionized water Add the starch solution to 450.0mL of boil-ing distilled/deionized water Add 10.0mL of solution 8 and the agar Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Cool

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850 Histoplasma capsulatum Broth

to 70°C Aseptically combine the two sterile solutions Pour into sterile

Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Histoplasma capsulatum

in the mycelial phase

Histoplasma capsulatum Broth

Glucose 10.0g

Citric acid 10.0g

Potato starch 0.5g

L-Asparagine 0.1g

L-Tryptophan 0.02g

Solution 1 250.0mL

Solution 3 40.0mL

Solution 2 10.0mL

Solution 4 10.0mL

Solution 5 1.0mL

Solution 8 1.0mL

Solution 6 0.1mL

Solution 7 0.1mL

pH 6.5 ± 0.2 at 25°C

Solution 1:

KH2PO4 8.0g

(NH4)2SO4 8.0g

MgSO4·7H2O 0.86g

CaCl2, anhydrous 0.08g

ZnSO4·7H2O 0.05g

Preparation of Solution 1: Add components to distilled/deionized

water and bring volume to 500.0mL Mix thoroughly Bring volume to

1.0L with distilled/deionized water Store at 5°C

Solution 2:

FeSO4·7H2O 5.7g

MnCl2·6H2O 0.8g

NaMoO4·2H2O 0.15g

HCl, concentrated 1.0mL

Preparation of Solution 2 : Add 1.0mL of concentrated HCl to

100.0mL of distilled water in a 1.0L volumetric flask Dissolve each

component completely in the sequence given Bring volume to 1.0L

with distilled/deionized water Store at 5°C Discard if red color or red

precipitate appears

Solution 3:

Preparation of Solution 3: Add casein to distilled/deionized water

and bring volume to 100.0mL Do not use enzymatically digested

ca-sein

Solution 4:

Calcium pantothenate 0.2g

Inositol 0.2g

Riboflavin 0.2g

Thiamine·HCl 0.2g

Nicotinamide 0.1g Biotin 0.01g

Solution 5:

Hemin 0.2g

at 5°C

Solution 6:

Preparation of Solution 6: Add DL-thioctic acid to 10.0mL of eth-anol Mix thoroughly Store solution at −20°C

Solution 7:

Coenzyme A 0.01g

Preparation of Solution 7: Prepare Na2S·5H2O solution in freshly boiled distilled/deionized water Add coenzyme A to 9.8mL of dis-tilled/deionized water Mix thoroughly Add freshly prepared

Solution 8:

Oleic acid 0.1g

Preparation of Solution 8: Add oleic acid to 50.0mL of distilled/de-ionized water Adjust pH to 9.0 with NaOH Gently heat until dissolved Bring volume to 100.0mL with distilled/deionized water Store at 5°C

Preparation of Medium: Add components—except potato starch and solution 8—to distilled/deionized water and bring volume to 400.0mL Mix thoroughly Adjust pH to 6.5 with 20% KOH solution Filter sterilize In a separate flask, add potato starch to 50.0mL of dis-tilled/deionized water Add the starch solution to 450.0mL of boiling distilled/deionized water Add 1.0mL of solution 8 Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 70°C Asepti-cally combine the two sterile solutions Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation of Histoplasma capsulatum in the yeast phase For the cultivation of Histoplasma duboisii, Blastomyces dermatitidis, and Sprotrichum schenckii.

HiVeg Hydrolysate Agar with 2.5% Agar Compositionper liter:

Agar 25.0g Plant hydrolysate 5.0g Plant peptone 5.0g NaCl 5.0g

Na2HPO4 2.5g Plant infusion 1.5g Yeast autolysate 1.5g Glycerol 22.0mL

pH 7.8 ± 0.2 at 25°C

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HNS Agar 851

Source: This medium, without glycerol, is available as a premixed

powder from HiMedia

to boiling Autoclave for 15 min at 15 psi pressure–121°C Mix

thor-oughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation of Vibrio cholerae For the production of

cholera vaccine

HiVeg Magnesium Broth Compositionper liter:

Plant hydrolysate 10.0g

NaCl 5.0g

MgSO4 0.94g

pH 7.0 ± 0.2 at 25°C

Hi-Media

thor-oughly

Use: For the cultivation of recombinant strains of Escherichia coli

HiVeg Peptone Water Compositionper liter:

Plant peptone 10.0g

NaCl 5.0g

pH 7.2 ± 0.2 at 25°C

Hi-Media

thor-oughly

Use: For the cultivation of various bacteria

HL Agar Composition per plate:

Columbia agar base 10.0mL

Columbia blood top agar 5.0mL

pH 7.3 ± 0.2 at 25°C

Columbia Agar Base:

Agar 13.5g

Pancreatic digest of casein 12.0g

NaCl 5.0g

Beef extract 3.0g

Yeast extract 3.0g

Cornstarch 1.0g

Preparation of Columbia Agar Base: Add components to

dis-tilled/deionized water and bring volume to 1.0L Mix thoroughly

Gen-tly heat until boiling Autoclave for 15 min at 15 psi pressure–121°C

Cool to 45°–50°C

Columbia Blood Top Agar:

Agar 13.5g Pancreatic digest of casein 12.0g NaCl 5.0g Peptic digest of animal tissue 5.0g Beef extract 3.0g Yeast extract 3.0g Cornstarch 1.0g Horse blood, defibrinated 50.0mL

Preparation of Columbia Blood Top Agar: Add components, except horse blood, to distilled/deionized water and bring volume to 950.0mL Mix thoroughly Gently heat until boiling Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add ster-ile horse blood Mix thoroughly

Preparation of Medium: Pour cooled, sterile Columbia agar base into sterile Petri dishes in 10.0mL volumes Allow agar to solidify Pour 5.0mL of cooled, sterile Columbia blood top agar over Columbia agar base that has solidified but is still warm

Use: For the cultivation of Listeria monocytogenes

HM Medium Compositionper liter:

NaCl 81.0g Yeast extract 10.0g MgSO4 9.6g MgCl2 7.0g Proteose peptone No 3 5.0g KCl 2.0g Glucose 1.0g CaCl2 0.36g NaHCO3 60.0mg NaBr 26.0mg

pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized wa-ter and bring volume to 1.0L Mix thoroughly Adjust pH to 7.1 Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of Salinicoccus roseus and Salinicoccus

hispani-cus.

HNS Agar (ATCC Medium 923) Compositionper liter:

Agar 15.0g NaCl 9.6g Heart infusion broth 990.0mL Horse serum 10.0mL

pH 7.4 ± 0.2 at 25°C

Heart Infusion Broth:

Beef heart, infusion from 500.0g Tryptose 10.0g NaCl 5.0g

Preparation of Heart Infusion Broth: Add agar and NaCl to 990.0mL heart infusion broth Mix thoroughly Autoclave for 15 min

at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 10.0mL sterile horse serum Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

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852 HNW Medium

Use: For the cultivation and maintenance of Corynebacterium species.

HNW Medium (DSMZ Medium 997) Composition per liter:

DMJ synthetic seawater 1.0L

Vitamin solution 10.0mL

NaHCO3solution 10.0mL

NaNO3solution 10.0mL

Na2S·9H2O solution 10.0mL

Tungstate solution 10.0mL

pH 7.2 ± 0.2 at 25°C

Tungstate Solution:

Na2WO4·2H2O 0.1mg

Preparation of Tungstate Solution: Add Na2WO4·2H2O to

dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly

NaHCO 3 Solution :

NaHCO3 1.0g

Preparation of NaHCO 3 Solution: Add NaHCO3 to

distilled/de-ionized water and bring volume to 10.0mL Mix thoroughly Sparge

with 20% CO2 + 80% H2 Filter sterilize

NaNO 3 Solution :

NaNO3 1.0g

Preparation of NaNO 3 Solution: Add NaNO3 to

distilled/deion-ized water and bring volume to 10.0mL Mix thoroughly Sparge with

100% N2 Filter sterilize

Vitamin Solution:

Pyridoxine-HCl 10.0mg

Thiamine-HCl·2H2O 5.0mg

Riboflavin 5.0mg

Nicotinic acid 5.0mg

D-Ca-pantothenate 5.0mg

p-Aminobenzoic acid 5.0mg

Lipoic acid 5.0mg

Biotin 2.0mg

Folic acid 2.0mg

Vitamin B12 0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L Mix thoroughly Sparge

with 80% H2 + 20% CO2 Filter sterilize

Na 2 S·9H 2 O Solution :

Na2S·9H2O 0.5g

Preparation of Na 2 S·9H 2 O Solution: Add Na2S·9H2O to

dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly

Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C Cool

to room temperature Adjust pH to 7.5

DMJ Synthetic Seawater:

Composition perliter:

NaCl 30.0g

MgCl2·6H2O 4.18g

MgSO4·7H2O 3.4g KCl 0.33g

NH4Cl 0.25g

K2HPO4 0.14g CaCl2·2H2O 0.14g Fe(NH4)2(SO4)2·6H2O 0.01g NiCl2·6H2O 0.5mg

Na2SeO3·5H2O 0.5mg Trace elements solution SL-10 10.0mL

Trace Elements Solution SL-10:

MgSO4·7H2O 3.0g Nitrilotriacetic acid 1.5g NaCl 1.0g MnSO4·2H2O 0.5g CoSO4·7H2O 0.18g ZnSO4·7H2O 0.18g CaCl2·2H2O 0.1g FeSO4·7H2O 0.1g NiCl2·6H2O 0.025g KAl(SO4)2·12H2O 0.02g

H3BO3 0.01g

Na2MoO4·4H2O 0.01g CuSO4·5H2O 0.01g

Na2SeO3·5H2O 0.3mg

Preparation of Trace Elements Solution SL-10: Add nitrilotri-acetic acid to 500.0mL of distilled/deionized water Dissolve by adjust-ing pH to 6.5 with KOH Add remainadjust-ing components Add distilled/ deionized water to 1.0L Mix thoroughly Adjust pH to 7.0

Preparation of DMJ Synthetic Seawater: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper-ature

Preparation of Medium: Aseptically add 10.0mL each of vitamin solution, NaHCO3solution, NaNO3solution, Na2S·9H2O solution, and tungstate solution to 1.0L sterile DMJ synthetic seawater Mix thor-oughly Distribute into tubes Tightly seal the tubes with butyl rubber stoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa)

Use: For the cultivation of Persephonella hydrogeniphila and

Hydro-genivirga caldilitoris.

Hofer’s Alkaline Medium Composition per liter:

Agar 15.0g Mannitol 10.0g Yeast extract 1.0g

K2HPO4 0.5g MgSO4 0.2g NaCl 0.1g Thymol Blue 0.016g

pH 11.0 ± 0.2 at 25°C

Use: For the selective isolation of Agrobacterium spp from soil

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Horikoshi-1 Medium with 10% Sodium Chloride 853

Hohn’s Medium, Modified

See: Steenken and Smith Agar

HO-LE Trace Elements Solution

H3BO3 2.85g

MnCl2·4H2O 1.8g

Sodium tartrate 1.77g

FeSO4 1.36g

CoCl2·6H2O 0.04g

CuCl2·2H2O 0.026g

Na2MoO4·2H2O 0.025g

ZnCl2 0.021g

water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For use as an enrichment to other media that require trace

miner-als

Hominis Agar

See: H Agar

Hominis Broth

See: H Broth

Horie Arabinose Ethyl Violet Broth

(HAEB) Compositionper liter:

NaCl 30.0g

Peptone 5.0g

Beef extract 3.0g

Bromthymol Blue 0.03g

Ethyl Violet 1.0mg

Arabinose solution 100.0mL

pH 9.0 ± 0.2 at 25°C

Arabinose Solution:

Arabinose 5.0g

Preparation of Arabinose Solution: Add arabinose to distilled/

deionized water and bring volume to 100.0mL Mix thoroughly Filter

sterilize

Preparation of Medium: Add components, except arabinose

solu-tion, to distilled/deionized water and bring volume to 900.0mL Mix

thoroughly Gently heat and bring to boiling Adjust pH to 9.0

Auto-clave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C

Asepti-cally add sterile arabinose solution Mix thoroughly AseptiAsepti-cally

distribute into sterile tubes or flasks

Use: For the cultivation of Vibrio species from foods.

Horikoshi Alkaline Medium

(DSMZ Medium 940) Compositionper liter:

Agar 15.0g

D-glucose 10.0g

Peptone 5.0g

Yeast extract 5.0g

Na2CO3 5.0g

KH2PO4 1.0g MgSO4·7H2O 0.2g

pH 9.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pannonibacter

phragmite-tus.

Horikoshi-1 Medium (DSMZ Medium 1081) Composition per liter:

Agar 15.0g Glucose 10.0g Polypeptone 5.0g Yeast extract 5.0g

KH2PO4 1.0g

K2HPO4 1.0g MgSO4·7H2O 0.2g NaCO3solution 100.0mL

pH 10.0 ± 0.2 at 25°C

NaCO 3 Solution :

NaCO3 10.0g

Preparation of NaCO 3 Solution: Add NaCO3 to distilled/deion-ized water and bring volume to 100.0mL Mix thoroughly Filter ster-ilize

Preparation of Medium: Add components, except NaCO3 solu-tion, to double distilled/deionized water and bring volume to 900.0mL Mix thoroughly Adjust pH to 10.0 Gently heat while stirring and bring

to boiling Mix thoroughly Autoclave for 15 min at 15 psi pressure– 121°C Cool to 50°C Aseptically add 100.0mL NaCO3 solution Ad-just pH to 10.0 Pour into Petri dishes or aseptically distribute into tubes

Use: For the cultivation of “Streptomyces sannurensis” and

Salinicoc-cus alkaliphilus

Horikoshi-1 Medium with 10% Sodium Chloride

(DSMZ Medium 1081a) Composition per liter:

NaCl 100.0g Agar 15.0g Glucose 10.0g Polypeptone 5.0g Yeast extract 5.0g

KH2PO4 1.0g

K2HPO4 1.0g MgSO4·7H2O 0.2g NaCO3solution 100.0mL

pH 10.0 ± 0.2 at 25°C

NaCO 3 Solution :

NaCO3 10.0g

Preparation of NaCO 3 Solution: Add NaCO3 to distilled/deion-ized water and bring volume to 100.0mL Mix thoroughly Filter ster-ilize

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854 Horse Blood Agar

Preparation of Medium: Add components, except NaCO3

solu-tion, to double distilled/deionized water and bring volume to 900.0mL

Mix thoroughly Adjust pH to 10.0 Gently heat while stirring and bring

to boiling Mix thoroughly Autoclave for 15 min at 15 psi pressure–

121°C Cool to 50°C Aseptically add 100.0mL NaCO3 solution

Ad-just pH to 10.0 Pour into Petri dishes or aseptically distribute into

tubes

Use: For the cultivation of Salinicoccus alkaliphilus

Horse Blood Agar Composition per liter:

Beef heart, infusion from 500.0g

Agar 15.0g

Tryptose 10.0g

NaCl 5.0g

Horse blood, defibrinated 50.0mL

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse blood, to

distilled/deionized water and bring volume to 950.0mL Mix

thorough-ly Gently heat and bring to boiling Autoclave for 15 min at 15 psi

pressure–121°C Cool to 45°–50°C Aseptically add sterile horse

blood Mix thoroughly Pour into sterile Petri dishes or distribute into

sterile tubes

Use: For the cultivation and maintenance of Yersinia

pseudotubercu-losis.

Horse Serum Agar Compositionper liter:

Agar 15.0g

Beef extract 3.0g

Horse serum 200.0mL

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to

se-rum Mix thoroughly Pour into sterile Petri dishes or distribute into

sterile tubes

Use: For the cultivation and maintenance of Pseudomonas aeruginosa

and Streptobacillus moniliformis.

Horse Serum Broth Compositionper liter:

Beef extract 3.0g

Horse serum 200.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD

Di-agnostic Systems

Preparation of Medium: Add components, except horse serum, to

se-rum Mix thoroughly Aseptically distribute into sterile tubes or flasks

Use: For the cultivation and maintenance of Pseudomonas aeruginosa

and Streptobacillus moniliformis.

Hottinger Broth Compositionper liter:

Fish peptone 20.0g Yeast extract 2.0g Tryptophan 1.0g

pH 7.2 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For cultivation of less fastidious microorganisms and determina-tion of indole as per USSR State Pharmacopoeia

Howardella Medium

(DSMZ Medium 1085) Composition per liter:

Casitone 20.0g Yeast extract 5.0g

Na2HPO4 5.0g MgCl2·6H2O 1.1g Urea 1.0g Na-thioglycolate 0.75g Resazurin 0.5mg Urea solution 10.0mL

pH 7.4 ± 0.2 at 25°C

Urea Solution :

Urea 1.0g

Preparation of Urea Solution: Add urea to distilled/deionized wa-ter and bring volume to 10.0mL Mix thoroughly Sparge with 100%

N2 Filter sterilize

Preparation of Medium: Add components, except thioglycolate and urea solution, to double distilled/deionized water and bring volume

to 990.0mL Cool to room temperature while sparging with 80% N2 + 20% CO2 Add thioglycolate Mix thoroughly Distribute into tubes or bottles under an atmosphere of 80% N2 + 20% CO2 Autoclave for 15 min at 15 psi pressure–121°C Cool to room temperature Aseptically add 10.0mL urea solution Adjust pH to 7.4

Use: For the cultivation of Howardella spp.

Hoyer’s Medium Compositionper liter:

(NH4)2SO4 1.0g

KH2PO4 0.9g MgSO4·7H2O 0.25g

K2HPO4 0.1g FeCl3·6H2O 0.02g Ethanol solution 200.0mL

Ethanol Solution:

Ethanol 30.0mL

Preparation of Ethanol Solution: Add ethanol to distilled/deion-ized water and bring volume to 200.0mL Mix thoroughly Filter ster-ilize

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