Handbook of Microbiological Media, Fourth Edition part 76 docx

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.. 0.5g Preparation of Solution A: Add components to distilled/deionized water and bring volume

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Glucose Nitrogen-Free Salt Agar 745

Glucose Cysteine HiVeg Agar Base

with Thiamine and Blood

Composition per liter:

Glucose 25.0g

Papaic digest of soybean meal 10.0g

Agar 14.0g

NaCl 5.0g

Plant peptone No 1 3.0g

Cysteine·HCl 1.0g

Thiamine 5.0mg

Blood, defibrinated 50.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium,without blood, is available as a premixed

pow-der from HiMedia

Preparation of Medium: Add components, except blood, to

dis-tilled/deionized water and bring volume to 950.0L Mix thoroughly

Gently heat until boiling Autoclave for 15 min at 15 psi pressure–

121°C Cool to 75°–80°C Add 50.0mL sterile defibrinated blood with

thorough mixing and maintain at 75°–80°C for 15–20 min until the

me-dium is chocolatized Pour into sterile Petri dishes or distribute into

sterile tubes

Use: For the cultivation and enumeration of Pasteurella tularensis.

Glucose HiVeg Broth

Plant hydrolysate 10.0g

Glucose 5.0g

NaCl 5.0g

Source: This medium is available from HiMedia

Preparation of Medium: Add components to distilled/deionized

water and bring volume to1.0L Mix thoroughly Gently heat and bring

to boiling Cool to 25° Adjust pH to 7.0 Distribute into sterile tubes or

flasks Autoclave for 15 min at 12 psi pressure–118°C

Use: For the study of glucose fermentation where pH indicator is not

desired

Glucose HiVeg Peptone Agar

Plant peptone 20.0g

Agar 15.0g

Glucose 10.0g

NaCl 5.0g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from

Hi-Media

water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15

psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of Agrobacterium species.

Glucose Medium Nakayama

(DSMZ Medium 452)

Composition per liter:

Yeast extract 15.0g

Glucose 10.0g

Peptone 10.0g

Agar 10.0g Solution A 10.0mL Solution B 10.0mL Solution C 1.0mL

pH 6.8 ± 0.2 at 25°C

Solution A:

Composition per 100.0mL:

KH2PO4 0.5g

K2HPO4 0.5g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 50°C

Solution B:

MgSO4·7H2O 3.0g NaCl 0.1g MnSO4·5H2O 0.1g CuSO4·5H2O 0.01g

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 100.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 50°C

Solution C:

Na3-citrate 2.0g FeSO4·7H2O 0.1g

Preparation of Solution C: Add components to distilled/deionized water and bring volume to 100.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure–121°C Cool to 50°C

Preparation of Medium: Add components, except solutions A, B, and C, to distilled/deionized water and bring volume to 979.0mL Mix thoroughly Adjust pH to 6.8 Gently heat and bring to boiling Auto-clave for 15 min at 15 psi pressure–121°C Cool to 50°C Aseptically add 10.0mL sterile solution A, 10.0mL sterile solution B, and 1.0mL sterile solution C Mix thoroughly Pour into sterile Petri dishes or aseptically dispense into sterile tubes

Use: For the growth and maintenance of Bacillus laevolacticus.

Glucose Nitrogen-Free Salt Agar

Agar 15.0g CaCO3 1.0g

K2HPO4 1.0g MgSO4·7H2O 0.2g NaCl 0.2g FeSO4·7H2O 0.1g

Na2MoO4·2H2O 5.0mg Glucose solution 100.0mL

pH 7.0 ± 0.2 at 25°C

Glucose Solution:

Composition per 100.0mL:

Glucose 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion-ized water and bring volume to 100.0mL Mix thoroughly Filter ster-ilize

Preparation of Medium: Add components, except glucose solu-tion, to distilled/deionized water and bring volume to 900.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at

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746 Glucose Nitrogen-Free Salt Solution

15 psi pressure–121°C Cool to 45°–50°C Aseptically add 100.0mL of

sterile glucose solution Mix thoroughly Pour into sterile Petri dishes

or distribute into sterile tubes

Use: For the cultivation of Azotobacter species.

Glucose Nitrogen-Free Salt Solution

CaCO3 1.0g

K2HPO4 1.0g

MgSO4·7H2O 0.2g

NaCl 0.2g

FeSO4·7H2O 0.1g

Na2MoO4·2H2O 5.0mg

Glucose solution 100.0mL

pH 7.0 ± 0.2 at 25°C

Glucose 10.0g

Preparation of Glucose Solution: Add glucose to

distilled/deion-ized water and bring volume to 100.0mL Mix thoroughly Filter

ster-ilize

Preparation of Medium: Add components, except glucose

solu-tion, to distilled/deionized water and bring volume to 900.0mL Mix

thoroughly Gently heat and bring to boiling Autoclave for 15 min at

15 psi pressure–121°C Cool to 45°–50°C Aseptically add 100.0mL of

sterile glucose solution Mix thoroughly Aseptically distribute into

sterile tubes or flasks

Use: For the cultivation of Azotobacter species.

Glucose Nutrient Agar

Agar 15.0g

Pancreatic digest of casein 10.0g

Glucose 5.0g

K2HPO4 5.0g

NaCl 5.0g

Yeast extract 5.0g

Use: For the isolation and cultivation of Brochothrix thermosphacta

Glucose Peptone Agar

Peptone 20.0g

Agar 15.0g

Glucose 10.0g

NaCl 5.0g

pH 7.2 ± 0.2 at 25°C

Use: For the cultivation of Agrobacterium species.

Glucose Peptone Medium

Agar 15.0g Glucose 0.3g Peptone 0.17g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Gently heat and bring

to boiling Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Acytostelium ellipticum,

Phlyctochytrium africanum, Rhizophylctis harderi, and Rhizophylctis rosea

Glucose Peptone Medium

Peptone 20.0g CaCO3 10.0g Glucose 10.0g Yeast extract 10.0g

Preparation of Medium: Add components to 1.0L of tap water Mix thoroughly Distribute into tubes or flasks Autoclave for 15 min

at 15 psi pressure–121°C

Use: For the cultivation and maintenance of Mycobacterium chitae.

Glucose Peptone Yeast Extract

Salts Medium

See: GPY Salts Medium

Glucose Phosphate Broth

Peptone 10.0g

K2HPO4 5.0g Glucose 5.0g

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components, except glucose, to dis-tilled/deionized water and bring volume to 1.0L Mix thoroughly Gen-tly heat and bring to boiling Filter while hot through Whatman filter paper Cool to 25°C Adjust pH to 7.5 Add 5.0g of glucose Mix thor-oughly Distribute into sterile tubes or flasks Autoclave for 10 min at

10 psi pressure–115°C

Use: For the cultivation of a variety of nonfastidious heterotrophic microorganisms

Glucose Phosphate Broth

Peptone 10.0g

K2HPO4 5.0g Glucose 5.0g

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components, except glucose, to dis-tilled/deionized water and bring volume to 1.0L Mix thoroughly Gen-tly heat and bring to boiling Filter while hot through Whatman filter paper Cool to 25°C Adjust pH to 7.5 Add 5.0g of glucose Mix thor-oughly Distribute into sterile tubes or flasks Autoclave for 10 min at

10 psi pressure–115°C

Use : For the cultivation of a variety of nonfastidious heterotrophic

mi-croorganisms

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Glucose Yeast Broth with Sodium Chloride 747

Glucose Salt Teepol Broth

(GSTB)

NaCl 30.0g

Peptone 10.0g

Glucose 5.0g

Beef extract 3.0g

Methyl Violet 2.0mg

Sodium lauryl sulfate

(Teepol—0.1% solution) 4.0mL

pH 8.8 ± 0.2 at 25°C

water and bring volume to 1.0L Mix thoroughly Adjust pH to 8.8

Dis-tribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–

121°C

Use: For the cultivation of Vibrio species from foods

Glucose Salt Teepol HiVeg Broth

NaCl 30.0g

Plant peptone 10.0g

Glucose 5.0g

Teepol 4.0g

Plant extract 3.0g

Methyl Violet 2.0mg

pH 8.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from

Hi-Media

121°C

Use: For the cultivation of Vibrio species from foods

Glucose Salts Medium

Glucose 5.0g

(NH4)2SO4 1.0g

MgSO4·7H2O 0.5g

NaCl 0.5g

NaH2PO4·12H2O 0.7mg

NaH2PO4·2H2O 0.3mg

Trace elements solution 0.5mL

pH 6.9 ± 0.2 at 25°C

Trace Elements Solution:

H3BO3 0.3g

CoCl2·6H2O 0.2g

ZnSO4·7H2O 0.1g

MnCl2·4H2O 0.03g

Na2MoO4·2H2O 0.03g

NiCl2·6H2O 0.02g

CuCl2·2H2O 0.01g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L Mix thoroughly

Filter sterilize

Preparation of Medium: Add components, except trace elements

solution, to distilled/deionized water and bring volume to 1.0L Mix

thoroughly Autoclave for 15 min at 15 psi pressure–121°C

Aseptical-ly add 0.5mL of sterile trace elements solution Mix thoroughAseptical-ly Asep-tically distribute into sterile tubes or flasks

Use: For the cultivation of a wide variety of bacteria and fungi

Glucose Starch Agar

Gelatin 20.0g Proteose peptone 15.0g Agar 10.0g Glucose 10.0g Starch, soluble 5.0g NaCl 5.0g

Na2HPO4 3.0g

pH 7.2 ± 0.2 at 25°C

Use: For use as a basal medium with the addition of salicin, raffinose,

and Phenol Red for the detection of Clostridium spp.

Glucose Tetrazolium Medium

Agar 15.0g Pancreatic digest of gelatin 6.0g Yeast extract 3.0g Beef extract 1.5g 1,3,5-Triphenyl tetrazolium chloride 0.05g Glucose solution 100.0mL

pH 6.6 ± 0.1 at 25°C

Glucose 10.0g

Preparation of Medium: Add components, except glucose solu-tion, to distilled/deionized water and bring volume to 900.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at

15 psi pressure–121°C Cool to 45°–50°C Aseptically add glucose so-lution Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation and maintenance of Streptococcus mutans.

Glucose Tryptone Yeast Extract Medium

See: GTYE Medium

Glucose Yeast Broth with Sodium Chloride

NaCl 50.0g Agar 15.0g Yeast extract 3.0g Glucose 1.0g

pH 7.0 ± 0.2 at 25°C

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748 Glucose Yeast Chalk Agar

Use: For the cultivation and maintenance of Pediococcus halophilus.

Glucose Yeast Chalk Agar

Chalk 40.0g

Agar 15.0g

Glucose 5.0g

Yeast extract 5.0g

Use: For the cultivation and maintenance of Xanthomonas species.

Glucose Yeast Extract Agar

Agar 15.0g

Glucose 5.0g

Meat extract 5.0g

Peptone 5.0g

Yeast extract 5.0g

pH 6.5 ± 0.2 at 25°C

Use: For the isolation and cultivation of Leuconostoc species and

Pediococcus species.

CaCO3 20.0g

Glucose 20.0g

Agar 17.0g

Yeast extract 10.0g

Use: For the cultivation of Agrobacterium species, Clostridium

spe-cies, Erwinia spespe-cies, Pseudomonas spespe-cies, and Xanthomonas

camp-estris.

Agar 15.0g

Peptic digest of animal tissue 5.0g

Yeast extract 5.0g

Glucose 2.0g

KH2PO4 0.5g

K2HPO4 0.5g

MgSO4·7H2O 0.3g

NaCl 0.01g

MnSO4·H2O 0.01g ZnSO4·7H2O 1.6mg CuSO4·5H2O 1.6mg CoSO4·7H2O 1.6mg

pH 7.2 ± 0.2 at 25°C

Use: For the enumeration and cultivation of lactobacilli in pharmaceu-tical preparations

Glucose Yeast Extract Iron Agar (LMG Medium 153)

Agar 15.0g Glucose 10.0g Yeast extract 5.0g Iron solution 20.0mL

pH 7.1 ± 0.2 at 25°C

Iron Solution:

FeCl3 0.03g

Preparation of Iron Solution: Add FeCl3 to distilled/deionized water and bring volume to 100.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except iron solution, to distilled/deionized water and bring volume to 980.0mL Mix

thorough-ly Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 20.0mL sterile iron solution Mix thoroughly Asepti-cally pour into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation of Pseudomonas denitrificans Note: Buffered Nutrient Agar is more commonly used for the culture of P denitrificans

Glucose Yeast Extract Medium (ATCC Medium 846)

Agar, noble 13.0g Yeast extract 5.0g

KH2PO4 1.0g NaCl 1.0g Peptone 1.0g

pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pseudomonas glathei.

Glucose Yeast Extract Medium (ATCC Medium 985)

Agar 15.0g Yeast extract 3.0g Glucose 1.0g

pH 7.0 ± 0.2 at 25°C

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Glucose Yeast Peptone Medium 749

Use: For the cultivation and maintenance of Acinetobacter

tartaro-genes, Agrobacterium viscosum, and Pseudomonas species.

Glucose Yeast Extract Medium

(ATCC Medium 1742)

Agar 15.0g

Glucose 5.0g

Yeast extract 3.5g

Use: For the cultivation and maintenance of Xanthobacter species.

Glucose Yeast Extract Peptone Agar

Glucose 20.0g

Agar 15.0g

Peptone 10.0g

Yeast extract 3.0g

pH 6.8 ± 0.2 at 25°C

Use: For the isolation and cultivation of yeasts from soils

Glucose Yeast Extract Peptone Agar with 2% Glucose

Glucose 20.0g

Agar 15.0g

Peptone 5.0g

Yeast extract 1.0g

K2HPO4 0.5g

MgSO4·7H2O 0.5g

CaCO3 1.0g

FeSO4·7H2O 50.0mg

pH 6.8 ± 0.2 at 25°C

Gently heat and bring to boiling Distribute into tubes or flasks

Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance of Lactobacillus acidophilus

and Leclercia adecarboxylata.

Glucose Yeast Extract Peptone Medium

(GYP Medium)

Glucose 20.0g

Agar 10.0g

Peptone 5.0g Yeast extract 5.0g CaCO3 0.1g

Use: For the isolation and cultivation of Sporolactobacillus species.

Glucose Yeast Extract Peptone Thioglycolate Medium

See: GYPT Medium

Glucose Yeast Medium with Calcium Carbonate

Agar 15.0g CaCO3 7.5g Peptone 5.0g Yeast extract 5.0g Glucose 3.0g

pH 7.0 ± 0.2 at 25°C

to boiling Distribute into tubes or flasks Adjust pH to 6.3 Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of Erwinia herbicola and

Bacillus species.

Glucose Yeast Plant Peptone Agar

Glucose 20.0g Agar 15.0g Plant peptone 10.0g Yeast extract 5.0g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-Media

Use: For the isolation of yeasts from soil specimens

Glucose Yeast Peptone Medium

Agar 20.0g Glucose 5.0g Peptone 5.0g Yeast extract 3.0g

pH 6.8 ± 0.2 at 25°C

to boiling Adjust pH to 6.8 Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance of a variety of yeasts,

includ-ing Candida species, Cryptococcus albidosimilis, Cryptococcus albidus,

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750 Glucose Yeast Peptone Medium

Cryptococcus laurentii, Cryptococcus vishniacii, Eeniella nana,

Filoba-sidiella neoformans, Histoplasma capsulatum, Kluyveromyces lactis,

Lipomyces kononenkoae, Rhodotorula matritensis, Saccharomyces

bayanus, Saccharomyces cerevisiae, Saccharomyces douglasii,

Saccha-romyces paradoxus, SchizosacchaSaccha-romyces pombe, and

Zygosaccharo-myces rouxii

Glucose Yeast Peptone Medium

Glucose 10.0g

Peptone 5.0g

Yeast extract 5.0g

pH 5.0 ± 1.0 at 25°C

121°C

Use: For the cultivation of Enterobacter cloacae.

Glutamate Medium

Solution A 500.0mL

Solution B 250.0mL

Solution C 250.0mL

Solution A:

Mannitol 10.0g

K2HPO4 0.22g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 500.0mL Mix thoroughly Autoclave for 15

min at 15 psi pressure–121°C Cool to 25°C

Solution B:

MgSO4·7H2O 0.1g

CaCl2·6H2O 0.08g

FeCl3·6H2O 0.05g

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 250.0mL Mix thoroughly Autoclave for 15

min at 15 psi pressure–121°C Cool to 25°C

Solution C:

Sodium glutamate 1.1g

Calcium pantothenate 0.5mg

Thiamine·HCl 0.1mg

Biotin 0.5μg

Preparation of Solution C: Add components to distilled/deionized

water and bring volume to 250.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Aseptically combine 500.0mL of cooled,

sterile solution A, 250.0mL of cooled, sterile solution B, and 250.0mL

of sterile solution C Mix thoroughly Aseptically distribute into sterile

tubes or flasks

Use: For the isolation of Rhizobium species.

Glutamate Medium (ATCC Medium 820)

Agar 15.0g

Sodium glutamate 5.0g

KH2PO4 1.0g MgSO4·7H2O 0.2g KCl 0.1g Glucose solution 100.0mL

pH 6.5 ± 0.2 at 25°C

Glucose 10.0g

Preparation of Medium: Add components, except glucose solution,

to distilled/deionized water and bring volume to 900.0mL Mix thor-oughly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool to 45°–50°C Aseptically add 100.0mL of sterile glucose solution Mix thoroughly Pour into sterile Petri dishes or distrib-ute into sterile tubes

Use: For the cultivation and maintenance of Pseudomonas species.

Glutamate Medium (ATCC Medium 1372)

K2HPO4 6.0g Sodium glutamate 5.0g Peptone 4.0g Yeast extract 4.0g

Use: For the cultivation and maintenance of Escherichia coli.

Glutamate Starch Phenol Red Agar Base

Starch, soluble 20.0g

L-Glutamate, sodium 10.0g Agar 12.0g

KH2PO4 2.0g MgSO4·7H2O 0.5g Phenol Red 0.36g Selective supplement solution 10.0mL

pH 7.2 ± 0.2 at 25°C

Selective Supplement Solution:

Penicillin G100,00 UPreparation of Selective Supplement So-lution: Add components to distilled/deionized water and bring volume to 10.0mL Mix thoroughly Filter sterilize

Preparation of Medium: Add components, except selective sup-plement solution, to distilled/deionized water and bring volume to 990.0mL Mix thoroughly Autoclave for 15 min at 15 psi pressure– 121°C Cool to 50°C Aseptically add selective supplement solution Mix thoroughly Pour into Petri dishes or aseptically distribute into sterile tubes

Use: For the detection of Pseudomonas spp and Aeromonas spp in

foodstuffs, wastewater, and equipment, in the food industry

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Glycerol Agar 751

Glutamine Medium

Agar 15.0g

Pancreatic digest of gelatin 5.0g

Beef extract 3.0g

Glutamine-tryptophan solution 10.0mL

pH 6.8 ± 0.2 at 25°C

Glutamine-Tryptophan Solution:

L-Glutamine 100.0mg

L-Tryptophan 100.0mg

Preparation of Medium: Add components, except

glutamine-tryp-tophan solution, to distilled/deionized water and bring volume to

990.0mL Mix thoroughly Gently heat while stirring and bring to

boil-ing Autoclave for 15 min at 15 psi pressure–121°C Aseptically add

10.0mL of sterile glutamine-tryptophan solution Mix thoroughly Pour

into sterile Petri dishes or distribute into sterile tubes

Use: For the cultivation of Escherichia coli.

Glutarate Medium

Sodium glutarate 2.6g

NaCl 1.0g

KCl 0.5g

MgCl2·6H2O 0.4g

NH4Cl 0.25g

KH2PO4 0.2g

CaCl2·2H2O 0.15g

Resazurin 1.0mg

Rumen fluid 20.0mL

NaHCO3 solution 20.0mL

Na2S·9H2O solution 10.0mL

Trace elements solution SL-10 1.0mL

pH 7.2 ± 0.2 at 25°C

Trace Elements Solution SL-10:

FeCl2·4H2O 1.5g

CoCl2·6H2O 190.0mg

MnCl2·4H2O 100.0mg

ZnCl2 70.0mg

Na2MoO4·2H2O 36.0mg

NiCl2·6H2O 24.0mg

H3BO3 6.0mg

CuCl2·2H2O 2.0mg

HCl (25% solution) 10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution Mix thoroughly Add distilled/deionized

water and bring volume to 1.0L Add remaining components Mix

thor-oughly Gas under 80% N2 + 20% CO2 Autoclave for 15 min at 15 psi

pressure–121°C

NaHCO 3 Solution:

NaHCO3 2.5g

Preparation of NaHCO 3 Solution: Add NaHCO3 to

distilled/de-ionized water and bring volume to 20.0mL Mix thoroughly Filter

ster-ilize Gas under 80% N2 + 20% CO2

Na 2 S·9H 2 O Solution:

Na2S·9H2O 0.36g

Preparation of Na 2 S·9H 2 O Solution: Add Na2S·9H2O to dis-tilled/deionized water and bring volume to 10.0mL Mix thoroughly Gas under 100% N2 Autoclave for 15 min at 15 psi pressure–121°C

Preparation of Medium: Prepare and dispense medium under 80%

N2 + 20% CO2 Add components, except NaHCO3 solution and

Na2S·9H2O solution, to distilled/deionized water and bring volume to 970.0mL Mix thoroughly Sparge with 80% N2 + 20% CO2 Autoclave for 15 min at 15 psi pressure–121°C Aseptically and anaerobically add 20.0mL of sterile NaHCO3 solution and 10.0mL of sterile Na2S·9H2O solution Mix thoroughly Aseptically and anaerobically distribute into sterile tubes or flasks

Use: For the cultivation of a wide variety of bacteria that can utilize glutarate as a carbon source

Glycerol Agar

Agar 15.0g Peptone 5.0g Beef extract 3.0g Soil extract 1.0L Glycerol 70.0mL

pH 7.0 ± 0.2 at 25°C

Soil Extract:

Soil, air dried 1.0Kg

Preparation of Soil Extract: Sift soil through a #9 mesh screen Add to 2.4L of tap water Mix thoroughly Autoclave for 60 min at 15 psi pressure–121°C Cool to 25°C Filter through Whatman #1 filter paper Bring volume to 1.0L with tap water

Preparation of Medium: Combine components Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pres-sure–121°C Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the selective isolation and cultivation of Nocardia species and Rhodococcus species.

Glycerol Agar

Beef heart, solids from infusion 250.0g Glycerol 60.0g Agar 15.0g Pancreatic digest of gelatin 5.0g Tryptose 5.0g Beef extract 3.0g NaCl 2.5g

pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Bacillus subtilis,

Entero-coccus faecalis, Erwinia chrysanthemi, Gordona rubropertinctus, Mycobacterium species, Nocardia brevicatena, Rhodococcus equi, and Rhodococcus rhodochrous.

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752 Glycerol Agar

Glycerol Agar

Beef heart, infusion from 300.0g

Glycerol 60.0g

Agar 17.5g

Tryptose 7.0g

NaCl 3.0g

Peptone 2.5g

NaCl 2.5g

Yeast extract 1.0g

Beef extract 0.5g

pH 7.3 ± 0.2 at 25°C

Gently heat and bring to boiling Distribute into tubes or flasks

Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation of fastidious bacteria

Glycerol Arginine Agar

Agar 15.0g

Glycerol 12.5g

Arginine 1.0g

K2HPO4 1.0g

NaCl 1.0g

MgSO4·7H2O 0.5g

Fe2(SO4)3·6H2O 0.01g

CuSO4·5H2O 1.0mg

MnSO4·H2O 1.0mg

ZnSO4·7H2O 1.0mg

Use: For the selective isolation and cultivation of streptomycetes

Glycerol Asparagine Agar

See: ISP 5

Glycerol Asparagine Meat Agar

Agar 20.0g

Glycerol 10.0g

Beef extract 10.0g

L-Asparagine 1.0g

K2HPO4 1.0g

Trace salts solution 1.0mL

pH 7.4 ± 0.2 at 25°C

Trace Salts Solution:

FeSO4·7H2O 0.1g

MnCl2·4H2O 0.1g

ZnSO4·7H2O 0.1g

Preparation of Trace Salts Solution: Add components to

dis-tilled/deionized water and bring volume to 100.0mL Mix thoroughly

Filter sterilize

Preparation of Medium: Add components, except trace salts solu-tion, to distilled/deionized water and bring volume to 999.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at

15 psi pressure–121°C Cool to 45°–50°C Aseptically add 1.0mL of sterile trace salts solution Mix thoroughly Pour into sterile Petri dishes

or distribute into sterile tubes

Use: For the cultivation of Actinomadura species and Streptomyces

species

Glycerol Asparagine Medium

Agar 20.0g Glycerol 10.0g

K2HPO4 1.0g

L-Asparagine 1.0g Trace salts solution 1.0mL

pH 7.2–7.4 at 25°C

Trace Salts Solution:

FeSO4·7H2O 0.1g MnCl2·4H2O 0.1g ZnSO4·7H2O 0.1g

Preparation of Trace Salts Solution: Add components to dis-tilled/deionized water and bring the volume to 100.0mL Mix thor-oughly Filter sterilize

to boiling Adjust pH to 7.0–7.4 Distribute into tubes or flasks Auto-clave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes

or leave in tubes

Use: For the cultivation and maintenance of Streptomyces species.

Glycerol Beef Extract Medium

Agar 15.0g Peptone 10.0g NaCl 5.0g Beef extract 3.0g Glycerol 40.0mL

Use: For the cultivation and maintenance of Corynebacterium

alkanolyt-icum, Pseudomonas mallei, Pseudomonas pseudomallei, and Rhodococ-cus species.

Glycerol Calcium Malate Agar

Agar 15.0g Calcium malate 10.0g Glycerol 10.0g

K2HPO4 0.5g

NH4Cl 0.5g

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Glycerol Nutrient Agar 753

Use: For the cultivation and maintenance of Actinoplanes awajinensis,

Dactylosporangium aurantiacum, Dactylosporangium salmoneum,

Dactylosporangium thailandense, and Planobispora rosea.

Glycerol Chalk Agar

NaCl 30.0g

Agar 15.0g

Glycerol 10.0g

CaCO3 5.0g

Peptone 5.0g

Yeast extract 3.0g

to boiling Autoclave for 15 min at 15 psi pressure–121°C Pour into

sterile Petri dishes Swirl flask while dispensing medium to keep

CaCO3 in suspension

Use: For the cultivation of Photobacterium species and Lucibacterium

species

Glycerol Corn Steep Agar

Agar 12.0g

Corn steep powder 5.0g

Glycerol 5.0g

NaCl 3.0g

Peptone from casein 3.0g

Yeast extract 3.0g

Beef extract 1.0g

pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Saccharomonospora

glauca.

Glycerol-Enriched Medium

Glycerol 30.0g

Peptone 20.0g

Yeast extract 10.0g

psi pressure–121°C

Use: For the cultivation and maintenance of Saccharomyces

cerevi-siae.

Glycerol-Enriched Medium

with 2% Ethanol

Glycerol 30.0g

Peptone 20.0g

Yeast extract 10.0g

Ethanol (absolute) 20.0mL

Preparation of Medium: Add components, except ethanol, to dis-tilled/deionized water and bring volume to 980.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pres-sure–121°C Cool to room temperature Filter sterilize the ethanol Aseptically add 20.0mL of sterile ethanol to the basal medium Mix thoroughly Distribute into sterile flasks or tubes

Use: For the cultivation and maintenance of Kluyveromyces lactis.

Glycerol-Free Medium

L-Asparagine 4.0g

L-Glutamine 4.0g Monosodium glutamate 4.0g

Na2HPO4 2.5g Pancreatic digest of casein 1.0g

KH2PO4 1.0g Triton®WR 1339 0.25g Ferric ammonium citrate 0.05g CaCl2 1.0mg CuSO4 0.5mg ZnSO4 0.5mg

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L Mix thoroughly Distribute into tubes

or flasks Autoclave for 15 min at 15 psi pressure–121°C

Use: For the cultivation of Mycobacterium tuberculosis Bacille

Calmette-Guèrin (BCG) for vaccine production

Glycerol Glycine Agar

Agar 15.0g Glycine 2.5g

K2HPO4 1.0g NaCl 1.0g CaCO3 0.1g FeSO4 0.1g MgSO4 0.1g Glycerol 20.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except glycerol, to dis-tilled/deionized water and bring volume to 1.0L Mix thoroughly Gen-tly heat and bring to boiling Add glycerol Mix thoroughly Distribute into tubes or flasks Autoclave for 15 min at 15 psi pressure–121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation of Streptomyces species.

Glycerol Nutrient Agar

Agar 15.0g Peptone 5.0g NaCl 5.0g Yeast extract 2.0g Beef extract 1.0g Glycerol 10.0mL

Use: For the cultivation of Mycobacterium smegmatis.

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754 Glycerol Soil Agar

Glycerol Peptone Agar

See: GP Agar

Glycerol Soil Agar

Glycerol 20.0g

Agar 15.0g

Peptone 5.0g

Beef extract 3.0g

Soil extract 150.0mL

pH 7.0 ± 0.2 at 25°C

Soil Extract:

Soil 400.0g

Preparation of Soil Extract: Air dry garden soil and pass through

a sieve Weigh out 400.0g and add to 960.0mL of tap water Autoclave

for 60 min at 15 psi pressure–121°C Cool to room temperature and

al-low soil to settle out Decant supernatant solution Filter through paper

Distribute into flasks in 200.0mL volumes Autoclave for 15 min at 15

psi pressure–121°C

Preparation of Medium: Add components to tap water and bring

volume to 1.0L Mix thoroughly Gently heat and bring to boiling

121°C Pour into sterile Petri dishes or leave in tubes

Use: For the cultivation and maintenance ofActinomadura madurae,

Amycolata autotrophica, Amycolata saturnea, Gordona bronchialis,

Gordona terrae, Mycobacterium species, Nocardia species, and

Tsuka-murella paurometabolum.

Glycine Cycloheximide Phenol Red Agar

Solution B 800.0mL

Solution A 200.0mL

Solution A:

Glycine 10.0g

(NH4)2SO4 5.0g

KH2PO4 1.0g

MgSO4·7H2O 0.5g

NaCl 0.1g

CaCl2·2H2O 0.1g

DL-Methionine 0.02g

DL-Tryptophan 0.02g

L-Histidine·HCl 0.01g

Inositol 2.0mg

H3BO3 0.5mg

ZnSO4·7H2O 0.4mg

MnSO4·4H2O 0.4mg

Thiamine·HCl 0.4mg

Pyroxidine·HCl 0.4mg

Niacin 0.4mg

Calcium pantothenate 0.4mg

p-Aminobenzoic acid 0.2mg

Riboflavin 0.2mg

FeCl3 0.2mg

Na2MoO4·4H2O 0.2mg

KI 0.1mg

CuSO4·5H2O 0.04mg Folic acid 2.0μg Biotin 2.0μg Cycloheximide solution 1.6mL

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 200.0mL Mix thoroughly Filter sterilize

Cycloheximide Solution:

Cycloheximide 0.1g

Preparation of Cycloheximide Solution: Add cylcohexamide to distilled/deionized water and bring volume to 100.0mL Mix

thorough-ly Filter sterilize

Caution: Cycloheximide is toxic Avoid skin contact or aerosol for-mation and inhalation

Solution B:

Agar 20.0g Phenol Red solution 30.0mL

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 800.0mL Mix thoroughly Gently heat and bring to boiling Autoclave for 15 min at 15 psi pressure–121°C Cool

to 50°–55°C

Phenol Red Solution:

Phenol Red 0.5g

Preparation of Phenol Red Solution: Add Phenol Red to dis-tilled/deionized water and bring volume to 100.0mL Mix thoroughly

Preparation of Medium: To 800.0mL of sterile solution B, asepti-cally add 200.0mL of sterile solution A at 55°C Mix thoroughly Pour into sterile Petri dishes or distribute into sterile tubes

Use: For the selective cultivation and differentiation of fungi from clinical

and nonclinical specimens Cryptococcus neoformans turns the medium

bright red

Glycocholate Mineral Medium

Agar 15.0g

K2HPO4 3.5g (NH4)2SO4 2.0g Sodium glycocholate 2.0g

KH2PO4 1.5g MgSO4·7H2O 0.1g Yeast extract 0.1g CaCl2·2H2O 0.01g FeSO4·7H2O 0.5mg

pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pseudomonas putida and

Pseudomonas species.

GMC Medium

See: Gelatin Metronidazole Cadmium Medium

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