Luận văn thạc sĩ synthesis characterization and biological activity of nano sio2 material

TABLE OF CONTENTS CHAPTER 1 OVERVIEW OF RESEARC 1.1 Overview of bone tissuc forming techniques, 1.1.1 Structure and role of bones 1.1.2 Treatment of bone damage 1.1.3 Bone Tissuz Forming

HANOI UNIVERSITY OF SCIENCE & TECHNOLOGY HUST

Instructor: PGS TS, Nguyén Kim Nga

Instructor’s signature

School: Chemical Engineering

HANOL 10/2021

TIANOI UNIVERSITY OF SCIENCE & TECIINOLOGY IUST

SOCIALIST REPUBLIC OF VIETXAM

Independence — Freedom - Happiness

GRADUATION APPROVAL

Full name of thesis author: Yudy Paola Moreno Ganzalez

Research Title: Synthesis

, Characterization and Bioactivity of SiOz nanoparticles

Specialization: Inorganic Chemistry

Student Identification: CA19027

Author, Scientific Instructor and Dissertation Tudging Commuillee accept the author who has corrected and supplemented the thesis seeerding to the minutes of the meeting of the board of directors on the day sees With the

following contents:

Dey month year

Tustructor Thesis author

CHAIRMAN OF THE ASSOCIATION

LIST OF TABLES

Table 3.) Particle sive and CTAB concentration uscd for the synthesis of SiOz naro-

‘Table 3.2 Characteristic oscillations and corresponding wavermmbers in nano-S101, 44

Table 3.3 Results of pI measurements of Si02/PDLLA membrane mineralization culture

LIST OF TABLES

Table 3.) Particle sive and CTAB concentration uscd for the synthesis of SiOz naro-

‘Table 3.2 Characteristic oscillations and corresponding wavermmbers in nano-S101, 44

Table 3.3 Results of pI measurements of Si02/PDLLA membrane mineralization culture

Acknpwledgement

‘Lhe completion of this study could not have been possible without the expertise of Assoc Prof Dr Nguyễn Kim Nga, my thesis supervisor Thank you for pointing me towards the corrcel path and supporting with the guides for my work

1 want to express my deepest thanks to Hanai University of Science and ‘Tecnhology, for affording me the opportunity to advance my studies and have reached one more step up

in my carrer devclopmenl, My gratitude for having the chance to moot lecturers and teachers who with their careful guidance were valuable source of knowledge for my study both theoretically and practically To my colleagues, for all the patience, quidance and understading

Finally, and most importanlty, to my caring, loving and supportive family -Claudia, Laura and Ruud-, my heartfelt thanks, You were always avaliable whenever I need a helping hand and a word of encouragement Without you none of this would be possible

Student

Yudy Paola Morsna Gonvatcy

LIST OF SIGNS AND ABBREVIATIONS

BPMs: Bone Morphogenetic proteins

MIIC: Major Histocompatibility Complex

TCP: Tricalcium phosphate

BTE: Bone Tissne Engineering

CS: Chitosan

ALP: Alkaline phosphatase

MSCs: Mesenchymal Stem Cells

TCP: Tricalcium Phosphate

ECM: Extraczfldlar Malrix

SBF: Simulated Body Fluid

HAp: Hydroxyapatite

'TEOS: 'Tetraethyl orthosilieate

FE-SEM: Fiel Emission Scanning Electron Microscope

XRD: X-ray diffiuction

EDX: Energy Dispersive X-ray Spectroscopy

FT-IR: Fowier-Transform Infrared Spectroscopy

PDLLA: Poly(D, L-Lactic Acid)

CTAB: Cetyltrimethylammonium Bromide

Acknpwledgement

‘Lhe completion of this study could not have been possible without the expertise of Assoc Prof Dr Nguyễn Kim Nga, my thesis supervisor Thank you for pointing me towards the corrcel path and supporting with the guides for my work

1 want to express my deepest thanks to Hanai University of Science and ‘Tecnhology, for affording me the opportunity to advance my studies and have reached one more step up

in my carrer devclopmenl, My gratitude for having the chance to moot lecturers and teachers who with their careful guidance were valuable source of knowledge for my study both theoretically and practically To my colleagues, for all the patience, quidance and understading

Finally, and most importanlty, to my caring, loving and supportive family -Claudia, Laura and Ruud-, my heartfelt thanks, You were always avaliable whenever I need a helping hand and a word of encouragement Without you none of this would be possible

Student

Yudy Paola Morsna Gonvatcy

SUBJECT TO THESIS

Research Title: Synthesis, Characterization and Bioactivity of SiOz nanoparticles School: Chemistry

Specialization: Inorganic Chemistry

Instructor: PGS TS Nguyễn Kin Nga

Instructor

Sign and full name

Acknpwledgement

‘Lhe completion of this study could not have been possible without the expertise of Assoc Prof Dr Nguyễn Kim Nga, my thesis supervisor Thank you for pointing me towards the corrcel path and supporting with the guides for my work

1 want to express my deepest thanks to Hanai University of Science and ‘Tecnhology, for affording me the opportunity to advance my studies and have reached one more step up

in my carrer devclopmenl, My gratitude for having the chance to moot lecturers and teachers who with their careful guidance were valuable source of knowledge for my study both theoretically and practically To my colleagues, for all the patience, quidance and understading

Finally, and most importanlty, to my caring, loving and supportive family -Claudia, Laura and Ruud-, my heartfelt thanks, You were always avaliable whenever I need a helping hand and a word of encouragement Without you none of this would be possible

Student

Yudy Paola Morsna Gonvatcy

TABLE OF CONTENTS

CHAPTER 1 OVERVIEW OF RESEARC

1.1 Overview of bone tissuc forming techniques,

1.1.1 Structure and role of bones

1.1.2 Treatment of bone damage

1.1.3 Bone Tissuz Forming Techniques

1.2 Materials used in Bone Tissue Enginecring +

1.2.1 organic Biomaterials

1.2.2 Biodegradable polymers

1.2.3 Composite materials (1.2.3)

1.3 Overview of Silicon dioxide

1.3.1 Structure of Silica Oxide

1.3.2 Properties of SiOz nanoparticles

1.33 Applications of Silica Oxide

1.4 Methods to synthesis Silica Oxide

1.4.1 Sol-gel method

142 High temperature method -

1.43 Conventional chemical precipitation

1.4.4 Raverse microemulsion method

1.4.5 Hydrothermal method

CHAPTER 2 METHODOLOGY OR EXPERIMENTAL METHODS

2.1 Equipment and tools

2.2 Reagents and method

2.3.3 Infrared spectroscopy (FT-IR) -

2.3.4 Energy Dispersive X-Ray Specestopy EDN

2.3.5 Bioactivity of nano-SiO2 materials

CHAPTER 3 RESULTS AND DISCUSSIO:

3.4 Results of characterization of nano-SiO2 material:

3.1.1 E-SLM analysis result

3.1.2 XRD analysis result

3.1.3 FT-IR analysis result

3.1.4 EDX analysis result

3.1.5 Mechanism of SiO» particles formation

surfactant,

3.2 Biological activity of Si02-1 nanomaterials

TABLE OF CONTENTS

CHAPTER 1 OVERVIEW OF RESEARC

1.1 Overview of bone tissuc forming techniques,

1.1.1 Structure and role of bones

1.1.2 Treatment of bone damage

1.1.3 Bone Tissuz Forming Techniques

1.2 Materials used in Bone Tissue Enginecring +

1.2.1 organic Biomaterials

1.2.2 Biodegradable polymers

1.2.3 Composite materials (1.2.3)

1.3 Overview of Silicon dioxide

1.3.1 Structure of Silica Oxide

1.3.2 Properties of SiOz nanoparticles

1.33 Applications of Silica Oxide

1.4 Methods to synthesis Silica Oxide

1.4.1 Sol-gel method

142 High temperature method -

1.43 Conventional chemical precipitation

1.4.4 Raverse microemulsion method

1.4.5 Hydrothermal method

CHAPTER 2 METHODOLOGY OR EXPERIMENTAL METHODS

2.1 Equipment and tools

2.2 Reagents and method

2.3.3 Infrared spectroscopy (FT-IR) -

2.3.4 Energy Dispersive X-Ray Specestopy EDN

2.3.5 Bioactivity of nano-SiO2 materials

CHAPTER 3 RESULTS AND DISCUSSIO:

3.4 Results of characterization of nano-SiO2 material:

3.1.1 E-SLM analysis result

3.1.2 XRD analysis result

3.1.3 FT-IR analysis result

3.1.4 EDX analysis result

3.1.5 Mechanism of SiO» particles formation

surfactant,

3.2 Biological activity of Si02-1 nanomaterials

LIST OF TABLES

Table 3.) Particle sive and CTAB concentration uscd for the synthesis of SiOz naro-

‘Table 3.2 Characteristic oscillations and corresponding wavermmbers in nano-S101, 44

Table 3.3 Results of pI measurements of Si02/PDLLA membrane mineralization culture

LIST OF SIGNS AND ABBREVIATIONS

BPMs: Bone Morphogenetic proteins

MIIC: Major Histocompatibility Complex

TCP: Tricalcium phosphate

BTE: Bone Tissne Engineering

CS: Chitosan

ALP: Alkaline phosphatase

MSCs: Mesenchymal Stem Cells

TCP: Tricalcium Phosphate

ECM: Extraczfldlar Malrix

SBF: Simulated Body Fluid

HAp: Hydroxyapatite

'TEOS: 'Tetraethyl orthosilieate

FE-SEM: Fiel Emission Scanning Electron Microscope

XRD: X-ray diffiuction

EDX: Energy Dispersive X-ray Spectroscopy

FT-IR: Fowier-Transform Infrared Spectroscopy

PDLLA: Poly(D, L-Lactic Acid)

CTAB: Cetyltrimethylammonium Bromide

TABLE OF CONTENTS

CHAPTER 1 OVERVIEW OF RESEARC

1.1 Overview of bone tissuc forming techniques,

1.1.1 Structure and role of bones

1.1.2 Treatment of bone damage

1.1.3 Bone Tissuz Forming Techniques

1.2 Materials used in Bone Tissue Enginecring +

1.2.1 organic Biomaterials

1.2.2 Biodegradable polymers

1.2.3 Composite materials (1.2.3)

1.3 Overview of Silicon dioxide

1.3.1 Structure of Silica Oxide

1.3.2 Properties of SiOz nanoparticles

1.33 Applications of Silica Oxide

1.4 Methods to synthesis Silica Oxide

1.4.1 Sol-gel method

142 High temperature method -

1.43 Conventional chemical precipitation

1.4.4 Raverse microemulsion method

1.4.5 Hydrothermal method

CHAPTER 2 METHODOLOGY OR EXPERIMENTAL METHODS

2.1 Equipment and tools

2.2 Reagents and method

2.3.3 Infrared spectroscopy (FT-IR) -

2.3.4 Energy Dispersive X-Ray Specestopy EDN

2.3.5 Bioactivity of nano-SiO2 materials

CHAPTER 3 RESULTS AND DISCUSSIO:

3.4 Results of characterization of nano-SiO2 material:

3.1.1 E-SLM analysis result

3.1.2 XRD analysis result

3.1.3 FT-IR analysis result

3.1.4 EDX analysis result

3.1.5 Mechanism of SiO» particles formation

surfactant,

3.2 Biological activity of Si02-1 nanomaterials

TABLE OF CONTENTS

CHAPTER 1 OVERVIEW OF RESEARC

1.1 Overview of bone tissuc forming techniques,

1.1.1 Structure and role of bones

1.1.2 Treatment of bone damage

1.1.3 Bone Tissuz Forming Techniques

1.2 Materials used in Bone Tissue Enginecring +

1.2.1 organic Biomaterials

1.2.2 Biodegradable polymers

1.2.3 Composite materials (1.2.3)

1.3 Overview of Silicon dioxide

1.3.1 Structure of Silica Oxide

1.3.2 Properties of SiOz nanoparticles

1.33 Applications of Silica Oxide

1.4 Methods to synthesis Silica Oxide

1.4.1 Sol-gel method

142 High temperature method -

1.43 Conventional chemical precipitation

1.4.4 Raverse microemulsion method

1.4.5 Hydrothermal method

CHAPTER 2 METHODOLOGY OR EXPERIMENTAL METHODS

2.1 Equipment and tools

2.2 Reagents and method

2.3.3 Infrared spectroscopy (FT-IR) -

2.3.4 Energy Dispersive X-Ray Specestopy EDN

2.3.5 Bioactivity of nano-SiO2 materials

CHAPTER 3 RESULTS AND DISCUSSIO:

3.4 Results of characterization of nano-SiO2 material:

3.1.1 E-SLM analysis result

3.1.2 XRD analysis result

3.1.3 FT-IR analysis result

3.1.4 EDX analysis result

3.1.5 Mechanism of SiO» particles formation

surfactant,

3.2 Biological activity of Si02-1 nanomaterials

LIST OF TABLES

Table 3.) Particle sive and CTAB concentration uscd for the synthesis of SiOz naro-

‘Table 3.2 Characteristic oscillations and corresponding wavermmbers in nano-S101, 44

Table 3.3 Results of pI measurements of Si02/PDLLA membrane mineralization culture

SUBJECT TO THESIS

Research Title: Synthesis, Characterization and Bioactivity of SiOz nanoparticles School: Chemistry

Specialization: Inorganic Chemistry

Instructor: PGS TS Nguyễn Kin Nga

Instructor

Sign and full name

TABLE OF CONTENTS

CHAPTER 1 OVERVIEW OF RESEARC

1.1 Overview of bone tissuc forming techniques,

1.1.1 Structure and role of bones

1.1.2 Treatment of bone damage

1.1.3 Bone Tissuz Forming Techniques

1.2 Materials used in Bone Tissue Enginecring +

1.2.1 organic Biomaterials

1.2.2 Biodegradable polymers

1.2.3 Composite materials (1.2.3)

1.3 Overview of Silicon dioxide

1.3.1 Structure of Silica Oxide

1.3.2 Properties of SiOz nanoparticles

1.33 Applications of Silica Oxide

1.4 Methods to synthesis Silica Oxide

1.4.1 Sol-gel method

142 High temperature method -

1.43 Conventional chemical precipitation

1.4.4 Raverse microemulsion method

1.4.5 Hydrothermal method

CHAPTER 2 METHODOLOGY OR EXPERIMENTAL METHODS

2.1 Equipment and tools

2.2 Reagents and method

2.3.3 Infrared spectroscopy (FT-IR) -

2.3.4 Energy Dispersive X-Ray Specestopy EDN

2.3.5 Bioactivity of nano-SiO2 materials

CHAPTER 3 RESULTS AND DISCUSSIO:

3.4 Results of characterization of nano-SiO2 material:

3.1.1 E-SLM analysis result

3.1.2 XRD analysis result

3.1.3 FT-IR analysis result

3.1.4 EDX analysis result

3.1.5 Mechanism of SiO» particles formation

surfactant,

3.2 Biological activity of Si02-1 nanomaterials

Project Summary

In this work, the successful synthesis of SiOz nanoparticles by the hydrothermal technique with addition of optital conditions of cetyllrimethylammonium bromide (CTAB) is slucidaicd and defined The determination in the composition and characterization of the material is supported with 4 specific analysis methods (FE-SEM,

XRD, FI-IR, EDX) Additionally, the biomineralization capability of the SiO: nanorods

is confirmed through in viiro tests in simulated body fluid The main objective of this roscarch is achicved by showing that highly bioactive SiO: nanoparticles with suilable rod-like shapes can be easily prepared by the hydrothermal method, highlighting the roll

of silica Oxide as a potential nanomaterial for bone regeneration

Student

Yudy Paola Moreno Gonzalez

Acknpwledgement

‘Lhe completion of this study could not have been possible without the expertise of Assoc Prof Dr Nguyễn Kim Nga, my thesis supervisor Thank you for pointing me towards the corrcel path and supporting with the guides for my work

1 want to express my deepest thanks to Hanai University of Science and ‘Tecnhology, for affording me the opportunity to advance my studies and have reached one more step up

in my carrer devclopmenl, My gratitude for having the chance to moot lecturers and teachers who with their careful guidance were valuable source of knowledge for my study both theoretically and practically To my colleagues, for all the patience, quidance and understading

Finally, and most importanlty, to my caring, loving and supportive family -Claudia, Laura and Ruud-, my heartfelt thanks, You were always avaliable whenever I need a helping hand and a word of encouragement Without you none of this would be possible

Student

Yudy Paola Morsna Gonvatcy

Figure 1.4 Image of 31 Chitosan/Ilydroxyapatite (Cs/Ilap) scaffold Image adopted fiom

Figure 1.9 Hydroxyl carbonate apatite (HCA) growth on a GCs surface after immersion

Figure 1.10 Representation of two tetrahedral (4-sided pyramid) shape connected at the

Figue 1.1] Representation of amorphous SiO> and different polymorphs crystalline

SIO¬ 18

Figure 1.12 Diagram of the phase transition paths between silica polymorphs conrad

by tomperature

Figure 1.13 Process fo the production of pyrogenic SAS from precursors (SiCH), Thạc

igure 1.14 Extemal and intemal surfaces with silanols fonned reversibily by Trảng and condensation of sitoxancs, Image adaplad from Ref [50]

Figure 1.15 Types of silanol groups and siloxane bridges on the surface of maghoe silica with internal OH groups

Figure 1.16 Schematic ilustration of the

formation on the surface of silica-based malarials Image arlopted from Ref [45] 27 Figure 1.17 Flow chart of sol-gel proccss to synthesize silica nanoparticles Image

Figure 1.18 Mechanism of particle growth and structural finmework of nanosilica

Figure 1.19 High-lemporalure lame pyrolysis of fied silica forms weakly soluble, salid spherical nanoparticles, Image adopted from Ref [50] - „3i Figure 1.20 Schematic representation of the inicroemilsion method for synthesis of silica

Figure 1.21 General purpose autoclave popularly used for hydrothermal synthesis 34 Figuc 1.22 Representation of SiO» nanoparticles formation under hydrothermal

Figure 2.3 Pxpsrimental procedure for biicaclivity of nano-SiO2 particles 40

Tigurc 3.LFE-SEM images of sample M2 (0.02M CTAB) al differcnt magnifications a) Magnification 100k: b) Magnification 50K scscsestmenieneinentninieinreneneneind

TABLE OF CONTENTS

CHAPTER 1 OVERVIEW OF RESEARC

1.1 Overview of bone tissuc forming techniques,

1.1.1 Structure and role of bones

1.1.2 Treatment of bone damage

1.1.3 Bone Tissuz Forming Techniques

1.2 Materials used in Bone Tissue Enginecring +

1.2.1 organic Biomaterials

1.2.2 Biodegradable polymers

1.2.3 Composite materials (1.2.3)

1.3 Overview of Silicon dioxide

1.3.1 Structure of Silica Oxide

1.3.2 Properties of SiOz nanoparticles

1.33 Applications of Silica Oxide

1.4 Methods to synthesis Silica Oxide

1.4.1 Sol-gel method

142 High temperature method -

1.43 Conventional chemical precipitation

1.4.4 Raverse microemulsion method

1.4.5 Hydrothermal method

CHAPTER 2 METHODOLOGY OR EXPERIMENTAL METHODS

2.1 Equipment and tools

2.2 Reagents and method

2.3.3 Infrared spectroscopy (FT-IR) -

2.3.4 Energy Dispersive X-Ray Specestopy EDN

2.3.5 Bioactivity of nano-SiO2 materials

CHAPTER 3 RESULTS AND DISCUSSIO:

3.4 Results of characterization of nano-SiO2 material:

3.1.1 E-SLM analysis result

3.1.2 XRD analysis result

3.1.3 FT-IR analysis result

3.1.4 EDX analysis result

3.1.5 Mechanism of SiO» particles formation

surfactant,

3.2 Biological activity of Si02-1 nanomaterials

Figure 1.4 Image of 31 Chitosan/Ilydroxyapatite (Cs/Ilap) scaffold Image adopted fiom

Figure 1.9 Hydroxyl carbonate apatite (HCA) growth on a GCs surface after immersion

Figure 1.10 Representation of two tetrahedral (4-sided pyramid) shape connected at the

Figue 1.1] Representation of amorphous SiO> and different polymorphs crystalline

SIO¬ 18

Figure 1.12 Diagram of the phase transition paths between silica polymorphs conrad

by tomperature

Figure 1.13 Process fo the production of pyrogenic SAS from precursors (SiCH), Thạc

igure 1.14 Extemal and intemal surfaces with silanols fonned reversibily by Trảng and condensation of sitoxancs, Image adaplad from Ref [50]

Figure 1.15 Types of silanol groups and siloxane bridges on the surface of maghoe silica with internal OH groups

Figure 1.16 Schematic ilustration of the

formation on the surface of silica-based malarials Image arlopted from Ref [45] 27 Figure 1.17 Flow chart of sol-gel proccss to synthesize silica nanoparticles Image

Figure 1.18 Mechanism of particle growth and structural finmework of nanosilica

Figure 1.19 High-lemporalure lame pyrolysis of fied silica forms weakly soluble, salid spherical nanoparticles, Image adopted from Ref [50] - „3i Figure 1.20 Schematic representation of the inicroemilsion method for synthesis of silica

Figure 1.21 General purpose autoclave popularly used for hydrothermal synthesis 34 Figuc 1.22 Representation of SiO» nanoparticles formation under hydrothermal

Figure 2.3 Pxpsrimental procedure for biicaclivity of nano-SiO2 particles 40

Tigurc 3.LFE-SEM images of sample M2 (0.02M CTAB) al differcnt magnifications a) Magnification 100k: b) Magnification 50K scscsestmenieneinentninieinreneneneind

SUBJECT TO THESIS

Research Title: Synthesis, Characterization and Bioactivity of SiOz nanoparticles School: Chemistry

Specialization: Inorganic Chemistry

Instructor: PGS TS Nguyễn Kin Nga

Instructor

Sign and full name

TABLE OF CONTENTS

CHAPTER 1 OVERVIEW OF RESEARC

1.1 Overview of bone tissuc forming techniques,

1.1.1 Structure and role of bones

1.1.2 Treatment of bone damage

1.1.3 Bone Tissuz Forming Techniques

1.2 Materials used in Bone Tissue Enginecring +

1.2.1 organic Biomaterials

1.2.2 Biodegradable polymers

1.2.3 Composite materials (1.2.3)

1.3 Overview of Silicon dioxide

1.3.1 Structure of Silica Oxide

1.3.2 Properties of SiOz nanoparticles

1.33 Applications of Silica Oxide

1.4 Methods to synthesis Silica Oxide

1.4.1 Sol-gel method

142 High temperature method -

1.43 Conventional chemical precipitation

1.4.4 Raverse microemulsion method

1.4.5 Hydrothermal method

CHAPTER 2 METHODOLOGY OR EXPERIMENTAL METHODS

2.1 Equipment and tools

2.2 Reagents and method

2.3.3 Infrared spectroscopy (FT-IR) -

2.3.4 Energy Dispersive X-Ray Specestopy EDN

2.3.5 Bioactivity of nano-SiO2 materials

CHAPTER 3 RESULTS AND DISCUSSIO:

3.4 Results of characterization of nano-SiO2 material:

3.1.1 E-SLM analysis result

3.1.2 XRD analysis result

3.1.3 FT-IR analysis result

3.1.4 EDX analysis result

3.1.5 Mechanism of SiO» particles formation

surfactant,

3.2 Biological activity of Si02-1 nanomaterials

Acknpwledgement

‘Lhe completion of this study could not have been possible without the expertise of Assoc Prof Dr Nguyễn Kim Nga, my thesis supervisor Thank you for pointing me towards the corrcel path and supporting with the guides for my work

1 want to express my deepest thanks to Hanai University of Science and ‘Tecnhology, for affording me the opportunity to advance my studies and have reached one more step up

in my carrer devclopmenl, My gratitude for having the chance to moot lecturers and teachers who with their careful guidance were valuable source of knowledge for my study both theoretically and practically To my colleagues, for all the patience, quidance and understading

Finally, and most importanlty, to my caring, loving and supportive family -Claudia, Laura and Ruud-, my heartfelt thanks, You were always avaliable whenever I need a helping hand and a word of encouragement Without you none of this would be possible

Student

Yudy Paola Morsna Gonvatcy

Acknpwledgement

‘Lhe completion of this study could not have been possible without the expertise of Assoc Prof Dr Nguyễn Kim Nga, my thesis supervisor Thank you for pointing me towards the corrcel path and supporting with the guides for my work

1 want to express my deepest thanks to Hanai University of Science and ‘Tecnhology, for affording me the opportunity to advance my studies and have reached one more step up

in my carrer devclopmenl, My gratitude for having the chance to moot lecturers and teachers who with their careful guidance were valuable source of knowledge for my study both theoretically and practically To my colleagues, for all the patience, quidance and understading

Finally, and most importanlty, to my caring, loving and supportive family -Claudia, Laura and Ruud-, my heartfelt thanks, You were always avaliable whenever I need a helping hand and a word of encouragement Without you none of this would be possible

Student

Yudy Paola Morsna Gonvatcy

SUBJECT TO THESIS

Research Title: Synthesis, Characterization and Bioactivity of SiOz nanoparticles School: Chemistry

Specialization: Inorganic Chemistry

Instructor: PGS TS Nguyễn Kin Nga

Instructor

Sign and full name

Acknpwledgement

‘Lhe completion of this study could not have been possible without the expertise of Assoc Prof Dr Nguyễn Kim Nga, my thesis supervisor Thank you for pointing me towards the corrcel path and supporting with the guides for my work

1 want to express my deepest thanks to Hanai University of Science and ‘Tecnhology, for affording me the opportunity to advance my studies and have reached one more step up

in my carrer devclopmenl, My gratitude for having the chance to moot lecturers and teachers who with their careful guidance were valuable source of knowledge for my study both theoretically and practically To my colleagues, for all the patience, quidance and understading

Finally, and most importanlty, to my caring, loving and supportive family -Claudia, Laura and Ruud-, my heartfelt thanks, You were always avaliable whenever I need a helping hand and a word of encouragement Without you none of this would be possible

Student

Yudy Paola Morsna Gonvatcy

SUBJECT TO THESIS

Research Title: Synthesis, Characterization and Bioactivity of SiOz nanoparticles School: Chemistry

Specialization: Inorganic Chemistry

Instructor: PGS TS Nguyễn Kin Nga

Instructor

Sign and full name

Figure 1.4 Image of 31 Chitosan/Ilydroxyapatite (Cs/Ilap) scaffold Image adopted fiom

Figure 1.9 Hydroxyl carbonate apatite (HCA) growth on a GCs surface after immersion

Figure 1.10 Representation of two tetrahedral (4-sided pyramid) shape connected at the

Figue 1.1] Representation of amorphous SiO> and different polymorphs crystalline

SIO¬ 18

Figure 1.12 Diagram of the phase transition paths between silica polymorphs conrad

by tomperature

Figure 1.13 Process fo the production of pyrogenic SAS from precursors (SiCH), Thạc

igure 1.14 Extemal and intemal surfaces with silanols fonned reversibily by Trảng and condensation of sitoxancs, Image adaplad from Ref [50]

Figure 1.15 Types of silanol groups and siloxane bridges on the surface of maghoe silica with internal OH groups

Figure 1.16 Schematic ilustration of the

formation on the surface of silica-based malarials Image arlopted from Ref [45] 27 Figure 1.17 Flow chart of sol-gel proccss to synthesize silica nanoparticles Image

Figure 1.18 Mechanism of particle growth and structural finmework of nanosilica

Figure 1.19 High-lemporalure lame pyrolysis of fied silica forms weakly soluble, salid spherical nanoparticles, Image adopted from Ref [50] - „3i Figure 1.20 Schematic representation of the inicroemilsion method for synthesis of silica

Figure 1.21 General purpose autoclave popularly used for hydrothermal synthesis 34 Figuc 1.22 Representation of SiO» nanoparticles formation under hydrothermal

Figure 2.3 Pxpsrimental procedure for biicaclivity of nano-SiO2 particles 40

Tigurc 3.LFE-SEM images of sample M2 (0.02M CTAB) al differcnt magnifications a) Magnification 100k: b) Magnification 50K scscsestmenieneinentninieinreneneneind

Project Summary

In this work, the successful synthesis of SiOz nanoparticles by the hydrothermal technique with addition of optital conditions of cetyllrimethylammonium bromide (CTAB) is slucidaicd and defined The determination in the composition and characterization of the material is supported with 4 specific analysis methods (FE-SEM,

XRD, FI-IR, EDX) Additionally, the biomineralization capability of the SiO: nanorods

is confirmed through in viiro tests in simulated body fluid The main objective of this roscarch is achicved by showing that highly bioactive SiO: nanoparticles with suilable rod-like shapes can be easily prepared by the hydrothermal method, highlighting the roll

of silica Oxide as a potential nanomaterial for bone regeneration

Student

Yudy Paola Moreno Gonzalez

Project Summary

In this work, the successful synthesis of SiOz nanoparticles by the hydrothermal technique with addition of optital conditions of cetyllrimethylammonium bromide (CTAB) is slucidaicd and defined The determination in the composition and characterization of the material is supported with 4 specific analysis methods (FE-SEM,

XRD, FI-IR, EDX) Additionally, the biomineralization capability of the SiO: nanorods

is confirmed through in viiro tests in simulated body fluid The main objective of this roscarch is achicved by showing that highly bioactive SiO: nanoparticles with suilable rod-like shapes can be easily prepared by the hydrothermal method, highlighting the roll

of silica Oxide as a potential nanomaterial for bone regeneration

Student

Yudy Paola Moreno Gonzalez

Project Summary

In this work, the successful synthesis of SiOz nanoparticles by the hydrothermal technique with addition of optital conditions of cetyllrimethylammonium bromide (CTAB) is slucidaicd and defined The determination in the composition and characterization of the material is supported with 4 specific analysis methods (FE-SEM,

XRD, FI-IR, EDX) Additionally, the biomineralization capability of the SiO: nanorods

is confirmed through in viiro tests in simulated body fluid The main objective of this roscarch is achicved by showing that highly bioactive SiO: nanoparticles with suilable rod-like shapes can be easily prepared by the hydrothermal method, highlighting the roll

of silica Oxide as a potential nanomaterial for bone regeneration

Student

Yudy Paola Moreno Gonzalez

Project Summary

In this work, the successful synthesis of SiOz nanoparticles by the hydrothermal technique with addition of optital conditions of cetyllrimethylammonium bromide (CTAB) is slucidaicd and defined The determination in the composition and characterization of the material is supported with 4 specific analysis methods (FE-SEM,

XRD, FI-IR, EDX) Additionally, the biomineralization capability of the SiO: nanorods

is confirmed through in viiro tests in simulated body fluid The main objective of this roscarch is achicved by showing that highly bioactive SiO: nanoparticles with suilable rod-like shapes can be easily prepared by the hydrothermal method, highlighting the roll

of silica Oxide as a potential nanomaterial for bone regeneration

Student

Yudy Paola Moreno Gonzalez

Project Summary

In this work, the successful synthesis of SiOz nanoparticles by the hydrothermal technique with addition of optital conditions of cetyllrimethylammonium bromide (CTAB) is slucidaicd and defined The determination in the composition and characterization of the material is supported with 4 specific analysis methods (FE-SEM,

XRD, FI-IR, EDX) Additionally, the biomineralization capability of the SiO: nanorods

is confirmed through in viiro tests in simulated body fluid The main objective of this roscarch is achicved by showing that highly bioactive SiO: nanoparticles with suilable rod-like shapes can be easily prepared by the hydrothermal method, highlighting the roll

of silica Oxide as a potential nanomaterial for bone regeneration

Student

Yudy Paola Moreno Gonzalez