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Collaboration for Agriculture and Rural Development CARD ProgramDEMONSTRATION OF SNAKEHEAD FISH IN TANKS AS A NEW MODEL OF THE TRADITIONAL VAC SYSTEM TO REDUCE ORGANIC POLLUTION AND IMPR

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Collaboration for Agriculture and Rural Development (CARD) Program

DEMONSTRATION OF SNAKEHEAD FISH IN TANKS AS A NEW MODEL OF THE TRADITIONAL VAC SYSTEM TO REDUCE ORGANIC POLLUTION AND IMPROVE INCOME FOR FARMERS IN THE CENTRAL COASTAL VIETNAM

Project title: Improving traditional integrated farming systems (VAC) - a new livelihood option for

poor farmers in the coastal communities

Project code: CARD 027/07 VIE

Author(s): Võ Văn Bình1, Đào Văn Phú1, Nguyễn Quang Chương1, Mai Văn Tài1, Assoc Prof

Ravi Fotedar2, Jane Fewtrell2

Project Implementing organisations:

1 Centre for Environment and Disease Monitoring in Aquaculture (CEDMA) – RIA1 2

Agriculture and Environment - Division of Science and Engineering-Curtin University of Technology, Australia

SUMMARY

In order to overcome the limitation of land available for aquaculture in the central costal region of Vietnam and to reduce organic pollution in traditional integrated fish farming, a new method was developed called the ‘improved VAC’ In traditional VAC systems the manure from farm animals is released into fish ponds whereas in the ‘Improved VAC’ the manure is used for earthworm culture The worm production is then used to feed high value fish species in tanks.

In this project snakehead fish (Channa maculatus) cultured in tanks and fed partly on earthworms, resulted in an increase in income generation for farmers The results have shown that the fish growth rate was relatively high, reaching 700 g/fish after 5 months The FCR for the entire production cycle was 5.22, 4.75 and 6.14 in demonstrations in Thanh Hoa, Ha Tinh and Quang Tri, respectively Water quality remained within the acceptable range for fish health with NH3 and NO2 levels in the tanks being significantly lower than those in ponds Economic analysis indicated that there was a high net profit in all demonstrations with a ROI of 71%, 104% and 41% in Thanh Hoa, Ha Tinh, and Quang Tri respectively.

The results demonstrate that culture of snakehead fish in tanks fed a partial diet of earthworms can increase income to farmers thus other farmers in the region could benefit from adoption of the model.

1 Introduction

Integrated farming systems encompassing

gardening, fish pond and livestock pen (under

Vietnamese acronym VAC) is the most

popular practice in the rural communities of

Vietnam and other countries in Asia including

China (Vincke, 1997) This farming model is

very efficient in terms of recycling of the

materials in the system (Bilard et al., 1990)

However, the productivity of the aquaculture

(pond) component is quite low ranging from a

few hundred kilograms to an average

1,500-1,700 kg per ha per year (0,015-0,017

kg/m2.year) (Kumar et.al, 2003) Aquatic

species produced in the traditional VAC

systems carry a high risk of contamination with organisms such as E.coli and parasites Acinetobacter spp that were exposed to the high organic load of the VAC system for two months and isolated from composite water-sediment samples were found to have an increased resistance to oxytetracycline and sulfamethoxazole (Andreas Petersen et al., 2002) E.coli , isolated from mothers involved

in integrated fish farming were found to have increased antibiotic resistance compared to those isolated from mothers who were not ( Kalter, 2009)

Therefore, it would be beneficial for the traditional VAC system to be improved to

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enable increased productivity and reduce

potential organic pollution In the Improved

VAC, manures from husbandry or by-product

of horticulture are used for the culture of

earthworms The earthworms produced are

then used as food for high value fish species

cultured in tanks and the faeces of the worm

is used as fertilizer

This article presents the results from

demonstration implementation of the Improved

VAC for the project CARD 027/07VIE It

focuses on the snakehead cultured in tanks fed

with trash fish and earthworms Examination

of fish growth rate, the water quality in tanks

and economic analysis of the demonstrations

are included

2 Materials and methods

The demonstrations of the Improved VAC

were implemented in three middle provinces of

the country: Quang Xuong - Thanh Hoa,

Thach Ha - Ha Tinh and Trieu Phong - Quang

Tri These sites are representative for three

socio-eco-systems The first site, Thanh Hoa,

is representative of a small household scale

(small garden and ponds with no artificial

aeration) The second site is for households

who have a higher standard of husbandry and

horticulture but no pond The third site was in

Quang Tri and is representative of households

with a large pond/s but poor water quality, and

basic horticulture and husbandry practices

The Improved VAC system includes 30 –

40m3 (height of 60 – 80 cm) of water in

concrete tanks or pond (100 – 300 m2), a

livestock pen with an area of 50-60m2 which

can produce manure for cultivation of earth

worms and a vegetable garden (Figure 1) The

fish tanks were surrounded with synthetic

polymer nets up to 2 m in height to prevent the

fish “jumping” out of the tanks when it rains

Indicators to assess efficiency of an Improved

VAC include culture water quality and profit

generation compared to a traditional VAC

Water quality is evaluated by using parameters

such as temperature, DO, NH3, NO2, NO3,

BOD, H2S In traditional VAC these

parameters are usually at the high end of

acceptable values in the range for growth for

aquatic animals

Fig 1 Flow of materials in an Improved VAC:

Manures from animal husbandry are not directly released into fish ponds Instead manure is used for earthworm culture.

During the production cycles, samples were collected for water quality analyses The sampling was conducted in different positions

in tanks, that is, close to the water inlet and outlet and in the centre part of the tank In the ponds two samples were taken, that is, near to the pump and in the centre of the pond Samples were then tested for NH3, NO2, pH,

DO and hardness by using test kits and laboratory analysis The results were interpreted in comparison with the traditional VAC and in accordance with the acceptable range for aquatic animals outlined in the National Standard for Water Quality (TCVN, 1995; 2000)

Snakehead (Channa maculatus) was stocked at density of 10 fish/m² The water level in tanks was maintained at 10 – 40 cm in depth, depending on the size of fish The system pump was switched on for 1 hour, twice a day

A partial water change was performed on the tanks after every four days of culture

Small snakehead were fed a diet that consisted mainly of earthworms, while larger fish were fed a diet that consisted of up to 70% trash fish To meet the system’s quantity requirements, earthworm cultures were initiated 1 month prior to stocking the system with fish

Garden (mainly vegetables) Pum

Manures for husbandry

or byproduct of horticulture

Cattle/pig/chicken shed (manures released)

Earth worm to culture

Semi-bio filter

Fish pond (or well to get water)

Concret

e tanks 20m2

Water circulated after treated

Earthworm shed (12 – 30 m2)

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Võ Văn Bình, Đào Văn Phú, Nguyễn Quang Chương, Mai Văn Tài, Ravi Fotedar & Jane Fewtrell

Economic analysis of the Improved VAC was

implemented by value estimation of input

(consisting of tanks depreciation, fingerlings,

feed), operation cost (pumping and labor

costs), and the value of output presented by

total production (from selling in markets, for

family or as gifts for relatives) multiplied by

the price at market

3 Results and discussions

3.1 Fish growth rate

In general, snakehead cultured in tanks in the

Improved VAC have grown fast, reaching 700

g/fish (approximately 40.5 cm in length) from

the initial size of 12 g (10 cm in length) after

about 5 months Fingerlings fed by ground

trash fish grew at the same rate as fingerlings

fed a combination of trash fish and earthworm

(with ratio of 50:50) However, the condition

factor K (K = W*100/L3) of the fish fed the

combined feed was higher than the fish fed on

trash fish only (1.20 compared to 1.12) This

indicates that earthworms are suitable food for snakehead

Food conversion rate (FCR) was calculated for the whole culture period in the three demonstrations in Thanh Hoa, Ha Tinh and Quang Tri These were 5.22, 4.75 and 6.14 respectively In the beginning of the production cycle, that is from fingerling to 300 g/fish, the FCRs for the combination feed of trash fish and earthworm (50:50) were 3.13; 3.66 and 4.11, respectively

Interestingly, in Ha Tinh the Improved VAC system was managed by well educated persons (both husband and wife were master and bachelor degrees) and the survival rate in this province was higher (97%) than in Thanh Hoa (92%) and Quang Binh (87%) where system managers had less formal education This suggests that system knowledge and understanding could be an important factor in the success of the operation

3.2 Water quality in tanks and ponds

The results clearly show that the semi-bio filter

operated efficiently, resulting in lower levels

of NO2 and NH3 in tanks than those in ponds

(Figure 3) NO2was lower than 0.1 mg/l in all

demonstration sites; which is within the

acceptable range for normal growth rate of

aquatic species, including snakehead fish

In Quang Tri, for instances, the level of NH4 and NO2were low in tanks while in ponds they were sometimes higher than the acceptable limit The value of NH4 and NO2 fluctuated between 0.36 – 0.66 mg/l and 0.004 – 0.03 mg/l, respectively (Figure 3)

Fig 2 Growth rate of snakehead fish in tanks in the

Trieu Phong – Quang Tri Improved VAC

demonstrations

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Fig 3 Comparison of total Ammonium and NO2 in ponds and in

tanks during the production cycle in the Quang Tri demonstration.

For snakehead, the concentration of dissolved

oxygen does not play such an important role as

many for other fish species due to the fact that

they possess an organ for breathing air

However, the concentration of NH4/NH3 and

NO2are critical issues to consider because they

are toxic to fish at low concentrations Both

trash fish and earthworms which were used to

feed the fish in these systems contain high protein levels which are quickly converted into

NH4/NH3 and NO2 within the system Therefore, levels of NH4/NH3 and NO2 must

be monitored and controlled

The parameters which are not content nitrogen original in production cycle are presented in Table 1

Table 1 Non-nitrogenous water quality measured during the culture of snakehead in Trieu Phong –

Quang Tri (P = Pond, and T = Tank)

Water quality parameters

Sampling months

Temperature (°C) 25.6 23.4 32.1 27.2 33.7 27.4 33.0 27.0 32.1 28.3 31.0 27.9

dH – Hardness (mg/l) 6.8 6.5 8.0 7.0 7.8 6.8 7.3 9.7 7.6 8.6 8.3 8.2

These parameters were well within the

favorable ranges for snakehead growth

However, it is important to monitor and

control these parameters as they (particularly

temperature and pH) influence the

concentration and availability of the

nitrogenous parameters Values of DO and

hardness were low, ranging between 3.4 – 4.4

mg/l and 6.5 – 8.6 respectively

3.3 Economic analysis

Economic analyses have shown a good profit from all demonstrations, regardless of the fact that, due to the time restrictions of the project, the statistics on which the analyses were based were limited to a one year production cycle From the results obtained from the demonstrations, it can be seen that the Improved VAC system has potential to

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Võ Văn Bình, Đào Văn Phú, Nguyễn Quang Chương, Mai Văn Tài, Ravi Fotedar & Jane Fewtrell

financially benefit farmers using the integrated

system in Central coastal provinces

The results of analysis have shown that the

average return on investment (ROI) of

snakehead culture in tanks was high,

suggesting that the model can generate income

and benefit to famers The demonstration in Ha

Tinh gave a ROI index of 104% (Table 2)

while in Thanh Hoa and Quang Tri were

lower, at 71% and 41% respectively (Table 3)

The largest proportion of expenditure was for

feed, occupying 47.1%; followed by tanks

depreciation (10.3%) and cost of the

fingerlings (8.2%) Earthworms replaced a

large portion of the feed so feed costs would

have been higher using the traditional VAC

Table 2 Economic analysis – detail of input

and output for the demonstration in Thạch Bàn – Thạch Hà – Hà Tĩnh

Tank depreciation (2 tanks x

20 m²)

1.000.000

Pumping cost (for circulation)

400.000

Fingerlings stocked (2000 fish)

800.000

Labor cost (for 4 months) 2.780.000

Table 3 Economic analyses of demonstrations in Thanh Hoa, Ha Tinh and Quang Tri provinces

3.4 Potential of snakehead culture in

tanks

3.4.1 Advantages and disadvantages

There are several advantages of culturing

snakehead in tanks:

1) When compared to pond aquaculture,

culture of fish in tanks requires a relatively

small area, thereby most households in the

middle coastal areas can easily include

aquaculture in their integrated farming

system

2) Trash fish, as feed for snakehead, is readily available in the coastal areas and it is relatively cheap Additionally, in the case of limited supply of trash fish (e.g weather disturbance such as a typhoon) earthworms from the Improved VAC can be used as an alternative feed

3) The culture conditions in tanks can be easily manipulated and therefore the alteration of cycles to suit individual situations and changes in the market is possible

4) Aquaculture in tanks requires relatively little water, thus, the operation cost for

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recirculation are reduced Also, in some

areas, for example in Quang Tri, water that

meets the standard of quality for

aquaculture is scarce, therefore effective

recirculating systems would be beneficial in

such areas

5) Snakehead can be stocked in tanks at high

density For example stocking densities of

up to 50 – 70 fish/m² are possible and can

give productivity of 40 – 60 kg/m² without

creating poor water quality in the system

However, the culture in tanks also has some

disadvantages The fish are easily harvested

which may lead to problems with theft, which

is common in rural areas Also, the culture of

snakehead in tanks requires relatively

advanced technology, so farmers must be

trained so that the system operates efficiently

and costs are kept to a minimum

3.4.2 Environmental aspect

a Within system

Snakehead are tolerant to low DO levels as

they possess air-breathing organs on sides of

the head (Munshi et al., 1994) However, with

high concentration of BOD and COD as found

in ponds, the fish will be susceptible to

Epizootic Ulcerative Syndrome (G N

Frerichs, 1993) A properly functioning

filtration system (i.e Improved VAC) will

address issues with dissolved organic material

in the water therefore, decreasing the

opportunity for disease to infect the culture

Iron levels are another concern for snakehead

in tanks Past experience and sampling has

shown that high iron levels in culture water

correlated with an increase on snakehead

mortality In addition, results suggest that the

growth rate of fish and FCR has a negative

correlation with increasing iron concentration

This was presented clearly in the Quang Tri

demonstration, where the iron concentration

was relatively high (> 3 mg/l)and the growth

performance and survival of the fish were low

when compared to those found in the systems

in Thanh Hoa and Ha Tinh

b Surrounding environment

Culturing snakehead in tanks has three major

advantages over pond aquaculture from an

environmental impact perspective;

1) Less effluent is released into the environment and, if release is necessary, it

is generally of a less polluting nature 2) Less water is required which is beneficial

in areas of limited water supply

3) Less physical damage to the environment resulting from construction

Furthermore, culturing snakehead in tanks is clean and is not associated with the bad odor that can accompany animal husbandry

3.4.3 Economic aspect

The economic efficiency of snakehead culture

in tanks is assessed based on the margin of the production cost and production value at market Unlike many fish species, the price gap of snakehead between farm gate and end users is not very high because snakehead can

be transported and kept very easily without mortality Despite this the economic efficiency

of the culture is very high More profit would

be possible if the farmers were to sell their produce directly to restaurants

The economic analysis of the culture of snakehead in tanks eliminated the notion that aquaculture is only possible in ponds Farmers now have evidence that they can include aquaculture into their integrated farming system with space of just 50 m²

4 Conclusions and recommendations

1 It has been shown that snakeheads have

a high growth rate in tanks, reaching to

700 g/fish after 5 months culture

2 From demonstrations, it can be concluded that the Improved VAC system is suitable for most situations in the central coastal area of Vietnam It would be beneficial for this model to be transferred to other farmers through the extension system

3 Water quality parameters tested during the culture indicated a suitable range for growth of aquatic animals, including for snakehead fish

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Võ Văn Bình, Đào Văn Phú, Nguyễn Quang Chương, Mai Văn Tài, Ravi Fotedar & Jane Fewtrell

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1 Tiêu chuẩn Nhà Nước Việt Nam năm 1995

(TCVN 5942-1995, TCVN 5992- 1995, TCVN

5993- 1995, TCVN 5994 – 1995 và TCVN

5996 – 1995) và năm 2000 (TCVN 6774:2000).

2 Andreas Petersen, Jens Strodl Andersen,

Tawatchai Kaewmak, Temdoung Somsiri, và

Anders Dalsgaard, 2002 Impact of Integrated

Fish Farming on Antimicrobial Resistance in a

Pond Environment Applied and Environmental

Microbiology, December 2002, p 6036-6042,

Vol 68, No 12

3 Billard, R.N De Pauw, J.C Micha, C.

Salomoni, and J Verreth (1990) The impact of

aquaculture in rural management In:

Aquaculture Society Special Publ No 12, N.

De Pauw and R Billard (Eds) Bredene,

Belgium

4 G N Frerichs, S D Millar and Supranee

Chinabut, 1993 Clinical response of

snakeheads (Ophicephalus striatus) to

experimental infection with snakehead fish

rhabdovirus and snakehead cell line retrovirus Published by Elsevier Science B.V.

5 Henry D Kalter, Robert H Gilman, Lawrence

H Moulton, Anna R Cullotta, Lilia Cabrera, AND Billie Velapatiño, 2009 Risk Factors for

Antibiotic-Resistant Escherichia coli Carriage

in Young Children in Peru: Community-Based Cross-Sectional Prevalence Study Am J Trop Med Hyg., 82(5), 2010, pp 879-888.

6 Munshi JS, Roy PK, Ghosh TK, Olson KR.,

1994 Cephalic circulation in the air-breathing

snakehead fish, Channa punctata, C gachua,

and C marulius (Ophiocephalidae, Ophiocephaliformes). MeSH – Anat Rec Department of Zoology, Bhagalpur University, India

7 Vincke M M J., 1997 Integrated farming of fish and livestock: Present stutus and future development F.A.O Rome.

8 Michael Poxton, 2003 Water Quality In Aquaculture Farming Aquatic Animals and Plants Fishing (Ed by John S Lucas and Paul

C Southgate), pp 47-73 News Books a blackwell Publishing company.

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