Báo cáo nghiên cứu khoa học " Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs " ppt

Project Abstract The aims of this project were to develop capacity for FMD diagnosis, surveillance and control at both a laboratory and field level within the wider animal health network

Disease in Cattle and Pigs

Chris Morrissy

1

Submitted July 2010

Table of Contents

1 Institute Information 1

2 Project Abstract _ 2

3 Executive Summary 2

4 Introduction & Background 4

4.1 Objectives of the project _ 4 4.2 Implementation Approach and Strategy 4

7 Implementation & Sustainability Issues 9

1 Institute Information

Vietnamese Institution Regional Animal Health Office (RAHO-6)

Ho Chi Minh City South Vietnam

Vietnamese Project Team Leader Dr Dong Manh Hoa

Australian Organisation Australian Animal Health Laboratory

[AAHL] Private Bag 24 Geelong, VIC 3220 Australia

Australian Personnel Mr Chris Morrissy

Completion date (original) 01/06/2008

Completion date (revised)

Reporting period Project Completion Report

Contact Officer(s)

In Australia: Team Leader

Name: Mr Chris Morrissy Telephone: +61 3 5227 5000

Position: Supervisor Mammalian Virology Fax: +61 3 5227 5555

Organisation Australian Animal Health

Laboratory [AAHL]

Private Bag 24 Geelong, VIC 3220, Australia

Email: chris.morrissy@csiro.au

In Australia: Administrative Contact

Name: Mr Christopher Morrissy Telephone: +61 3 5227 5434

Position: Patents Contracts Officer Fax: +61 3 5227 5555

Organisation Australian Animal Health

Laboratory [AAHL]

Private Bag 24 Geelong, VIC 3220 Australia

Email: christopher.morrissy@

csiro.au

In Vietnam

Name: Dr Dong Manh Hoa Telephone: + 84 8 8568220

Position: Director Fax: + 84 8 8569050

Organisation Regional Animal Health Office

(RAHO-6)

Ho Chi Minh City South Vietnam

Email: rahchcmc@hcm.vnn.vn

2 Project Abstract

The aims of this project were to develop capacity for FMD diagnosis, surveillance and control at both a laboratory and field level within the wider animal health network

in Vietnam Improved diagnostic capacity will facilitate early detection and identification

of FMD enabling better disease control Specifically, successful capacity development

at regional laboratories will enable quality assured laboratory capability for FMDV diagnosis and serology This will be applied to the investigation of FMDV serotypes currently in circulation, vaccination coverage/response and the elucidation of on-going suspected FMD vaccine failures in Vietnam In addition, the successful establishment of an effective laboratory network for the diagnosis and control of FMD will provide a greater understanding of FMD epidemiology and will facilitate the coordination and implementation of effective vaccination control strategies via the wider animal health network in Vietnam

3 Executive Summary

On completion, this project has realised all laboratory-based objectives at a national level However, due to the lack of epidemiology support and the diversion of DAH resources to deal with outbreaks of AI and PRRSV, field-dependent objectives were only achieved to a varying extent throughout Vietnam as a whole Nevertheless all project objectives were fully realised and with great effect in southern Vietnam Despite considerable achievements on a regional basis, this project clearly demonstrates the consequences of not having a fully integrated FMD diagnostic and surveillance network at a national level In relation to FMD capacity development

and sustainability, one of the primary aims of this project was to “highlight the

importance of having an integrated laboratory network to identify what is happening

in the field and how to prevent and control outbreaks” This project on a national

scale was undoubtedly ambitious Indeed this was the intention and in this respect the project has been highly effective For example, the considerable success achieved in southern Vietnam has highlighted RAHO-6 as an exemplar to DAH In addition, the success achieved by RAHO-6 will act as an impetus to other regional offices in relation to the requirement for closer integration and clear, effective, two-way communication between laboratory and field-based personnel

During the course of the project there was a clear improvement and advances in both laboratory and field-based activities for the diagnosis and control of FMD The AAHL Scientific Coordinator has mentored and liaised extensively with four diagnostic laboratories; RAHO-6 [HCMC]; NCVD [Hanoi]; RAHO-7 [Can Tho]; RAHO-4 [Da Nang] and one research laboratory, NAVETCO [HCMC] As a result of successful capacity development and technology transfer from AAHL the RAHO-6 and NCVD laboratories now possess comprehensive, quality assured capabilities to diagnose FMD and perform sero-surveillance In addition, both laboratories are capable of performing FMD virus isolation, virus neutralisation, ELISAs, PCR and sequencing/genotyping for the characterisation of FMD field isolates All FMD laboratory diagnostic capabilities have been subject to internal quality assurance following on-site appraisal by the AAHL Scientific Coordinator using reference reagents supplied by AAHL and also external quality assurance using PT Panels from both AAHL and the FMD Regional Reference Laboratory in Thailand as detailed in earlier Milestone reports This has resulted in the recognition of both RAHO-6 and NCVD as FMD Reference Laboratories in Vietnam

In addition, quality assured FMD diagnostic capability in the form of the AAHL FMD

Ag ELISA [for the detection of virus] and both the AAHL FMD C-ELISA and ELISA [for post-vaccination surveillance] have been successfully established at both RAHO-4, RAHO-7 and NAVETCO

LP-Following the establishment of comprehensive FMD diagnostic, virus isolation and serotyping capability at RAHO-6 tests have been implemented with both zeal and determination and of particular significance, with a long-term strategic vision For example, the isolation and propagation of FMD serotypes in cell culture has been used by RAHO-6 to produce their own FMD antigens for in-house ELISA use and are distributing this antigen for use in other regional laboratories in Vietnam In addition, ongoing sero-surveillance, vaccine efficacy monitoring and the serotyping

of FMD field isolates has facilitated the evidence-based selection of the most appropriate FMD vaccine serotype composition and the identification of disease incursions from adjacent countries

As detailed in earlier Milestone Reports, central to the achievement of project objectives in regions such as southern Vietnam was the close integration of laboratory and field-based activities Overall, there was a significant improvement in the amount of data and quality of field specimens submitted to the laboratories over each surveillance round This reflected a greater appreciation of the importance of high quality field data and clinical specimens for FMD surveillance and control by regional laboratories The realisation of project objectives in regions such as southern Vietnam was greatly facilitated by the leadership, focus and vision of the main collaborating laboratory / office, RAHO-6 In particular, the establishment and support of an epidemiological unit at RAHO-6 as a result of this project has greatly assisted in the training of epidemiologists and field veterinarians in FMD sero-surveillance, outbreak control and disease investigation including vaccine failure During the course of this project, this closer integration, collaboration and communication between RAHO-6 laboratory / office and field personnel in southern Vietnam has facilitated the acquisition of the necessary high quality field data and clinical specimens This highly effective two-way integration has resulted in an every decreasing incidence of FMD outbreaks in southern Vietnam with only one isolated outbreak being detected in the 2009-2010 reporting period This significant achievement has been noted by DAH, attests to the effectiveness of the project implementation approach, and highlights the successful RAHO-6 “lab-to-field” and

“field-to-lab” integration as an exemplar to other regional laboratories / offices

Sero-surveillance and epidemiological studies have enabled the acquisition of important data in relation to vaccine coverage, the serotyping of FMD field isolates and the prevalence of FMD infection in Vietnam This data has enabled driven science-based changes in vaccine recommendations to be made with great effect in the field This project has not only been of benefit to Vietnam but has also lead to a greater knowledge and understanding of circulating FMDV genotypes and the benefits of sero-surveillance for the whole region In acknowledgement of the significance of this work, project participants have been invited to a number of regional [SEAFMD LabNet 2010; SEAFMD LMWG 2008] and international meetings [EU-FMD; OIE Subcommittee 2010] to present Vietnam’s highly effective implementation strategy for the control of FMD Of particular significance, it should

be noted that as a result of this project Vietnam is recognised internationally as model example to other counties in the region in relation to the successful implementation of FMD diagnostic tests, sero-surveillance, outbreak investigations and disease control

4 Introduction & Background

4.1 Objectives of the project:

1 To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance

2 To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies

Completing these objectives will improve the diagnostic capability of veterinary laboratories in Vietnam and achieve training of DAH veterinarians in disease investigation and control This will strengthen both the role and the profile of DAH which will play a vital role in making Vietnam more economically competitive In addition, improved animal health will lead to an increase in rural productivity though increased animal production Healthy animals will enable small farmers to be more competitive in the local market and the control of FMD will reduce poor farmers’ vulnerability to FMD outbreaks and result in a more stable income stream Establishing a diagnostic network which extends from the North to South Vietnam, from the laboratory to the farm level, reinforced by training and education, will give Vietnam a more integrated animal health network and greatly facilitate disease control This will directly increase the competitiveness and productivity of the national agricultural system which includes the major areas of concern including the Mekong Delta and the Central Coast

4.2 Implementation Approach and Strategy

The approach for technology transfer is well established at AAHL and has been successfully applied in previous projects in Vietnam, Thailand and Indonesia The project approach used was also thought to be the most appropriate for developing

an understanding of FMD epidemiology in Vietnam All studies were designed in conjunction with DAH with the aim to provide the critical information in relation to current FMD status in Vietnam and the effectiveness of FMD vaccine usage in the field The diagnostic technologies that will be used in this approach are the standard OIE diagnostic tests in use throughout the world to study, control and eradicate FMD

AAHL has extensive experience in FMD technology transfer and capacity development including the implementation of field surveys for the measurement of the prevalence of antibodies against FMD such as earlier ACIAR projects in Laos and Thailand

5 Progress to Date

5.1 Implementation Highlights [Upon Project Completion]

The main achievements of the project were:

• Successful capacity development for the diagnosis, surveillance and

control of FMD in Vietnam Specifically, the following quality assured FMD diagnostics were established in the collaborating laboratories:

RAHO6 and NCVD laboratories have established comprehensive cell culture, virus isolation, virus neutralisation test, ELISA, PCR, sequencing/genotyping capability

• It should be noted that both RAHO-6 and NCVD have achieved FMD Reference Laboratory status in Vietnam

RAHO4, RAHO7 and NAVETCO laboratories also have the capability for FMD diagnosis and serology by ELISA for post-vaccination surveillance

All laboratories in the project are able to utilise the AAHL LP-ELISA, ELISA and 3ABC ELISA to establish the sero-prevalence of FMD in herds and vaccine coverage post-vaccination

C-
All laboratories have implemented an in-house Quality Assurance system to monitor the accuracy of test results

• The project has both driven and inspired an increase in collaboration

between the Vietnamese laboratories and helped nurture a more integrated animal health laboratory network

This will be essential not only for the control of FMD and has since been applied to other animal diseases such as resent PRRSV outbreaks in Vietnam

• Improvement in the quality and number of samples submitted to the

laboratory for serotyping by ELISA

The closer integration of laboratory and field-based personnel, e.g as achieved by RAHO-6, combined with improved diagnostic capacity and capability throughout Vietnam has led to the serotyping of a greater number of clinical specimens at both RAHO-6 and NCVD

In addition, the significant improvement in sample collection and specimen submission has allowed virus isolation from field samples This was not been possible prior to the initiation of this project and of particular significance has enabled genotyping of FMDV field isolates

This has enabled DAH to achieve a greater understanding of the serotypes of FMD viruses circulating in Vietnam and provided essential baseline data in relation to the future control of FMD throughout Vietnam

• Genotyping and analysis of approx 100 Vietnamese FMD field isolates

collected from 2006 onwards

Genotyping data was detailed in Appendix 1 of the earlier Milestone 3 report

Sequence data was sent to WRL for additional confirmation and comparison to other FMD isolates

This information was shared with SEAFMD as part of the regional project to control FMD

Genotyping has elucidated the reason underlying vaccine failures in the field and has successfully established capability in Vietnam to determine the source of a FMD outbreak and to make evidence-based decisions in relation to whether the current vaccine serotype compositions are appropriate

Genotyping, combined with the ability to carry out VNT for determining antigenic variation of field isolates, has enabled Vietnamese laboratories

to investigate future vaccine failures

Of particular significance, this has already resulted in the elucidation of vaccine failures in the field as a result of inappropriate vaccine composition usage as detailed in the earlier Milestone 8 Report

• Standard forms and protocols for collection of field data and trained field

veterinarians in how to carry out disease investigation for control of disease and vaccine failure

• A model approach for a program to control FMD with a strong laboratory

and field component

• Throughout the project AAHL consultants have mentored and liaised

extensively with collaborating laboratories to achieve the following:

Established a comprehensive portfolio of internally and externally quality assured FMD diagnostic techniques at the newly established FMD Reference Laboratories at RAHO-6 and NCVD and related quality assured FMD ELISAs at all participating laboratories

Appraised quality assurance records and data collection under in-house

QA systems to ensure test records were being maintained and results were interpreted correctly

Established and appraised cell culture and virus isolation capability for growth of FMD isolates from the field which has enabled further genotyping of FMD field isolates

Validated an in-house ELISA using FMD antigen produced at RAHO-6 using Vietnam isolates The production and supply of this antigen to other laboratories facilitates future sustainability

Established and appraised molecular techniques to ensure best practice workflow for FMD diagnostic PCR under local conditions

Provided advice on the design and format of sample submission, data collection forms and outbreak investigation surveys to facilitate the acquisition of high quality field data and specimen submissions

Analysed and interpreted field and laboratory data

Supplied consumables, reference IQC reagents and EQA PT reagents

to confirm successful establishment of quality assured FMD diagnostic tests

On-going appraisal of laboratory quality assurance procedures and advice in relation to individual laboratory quality assurance manuals and SOPs

5.2 Smallholder Benefits

All pig and cattle and producers are potential beneficiaries To date, revised vaccine recommendations, made based on the serotyping and genotyping of Vietnamese FMDV field isolates have already resulted in improved vaccine strategies in the field and during the course of this project has facilitated a significant reduction in the incidence of FMD outbreaks in Vietnam On-going FMD surveillance, serotyping and genotyping of field isolates will continue to improve the effectiveness of tailored vaccination strategies in the field in Vietnam This will continue to be of financial benefit to small farmers through reduced losses due disease in their cattle and pigs DAH personnel, district veterinarians and farmers have gained improved knowledge and appreciation with regard to the selection of the most appropriate vaccine serotype[s] composition which will improve the efficacy of vaccination and the realisation of long-term goals in relation to the control of FMD in Vietnam

5.3 Capacity Building

This project has achieved the successful establishment of quality assured FMD diagnostic capability throughout Vietnam Of particular significance, both RAHO-6 and NCVD have established comprehensive quality assured FMD diagnostic capabilities and have subsequently attained FMD Reference Laboratory status in Vietnam In addition, RAHO6 and NCDV have also established virus isolation, cell culture, virus neutralisation and virus genotyping capabilities which will facilitate the on-going characterisation of FMDV field isolates In relation to capacity development at a national level, all collaborating laboratories have established FMD ELISA diagnostic capabilities for the diagnosis of FMD and for routine sero-surveillance

In relation to the achievement of long-term objectives, capacity development with regard to the training and education of field veterinarians in sample and data collection was of particular importance It was readily apparent that an integrated

“lab-to-field” and “field-to-lab” approach is a central logistical consideration in relation

to FMD control in the field The effectiveness of this approach was exemplified by the considerable successes in southern Vietnam, where greater two-way “lab-to-field” integration and cohesion resulted in an increase in both the quality and number

of samples collected and submitted to the laboratory which enabled the highly effective control of FMD in the field

This project has established a model for FMD control with the success achieved in southern Vietnam representing an exemplar to other regional laboratories and to the SEA region as a whole Nevertheless, of particular significance, this project has also highlighted some inherent shortcomings at a national level in relation to the logistics associated with the implementation of the National FMD Surveillance Program in Vietnam

5.4 Publicity

This CARD AusAID project has received highly favourable publicity in Vietnam, Australia and internationally and recognition of the achievements made in relation to the understanding and control of FMD in Vietnam FMD is a disease of importance

in Vietnam and of strategic significance not only to Australia and neighbouring countries in SEA but also worldwide As a result of this CARD AusAID and earlier ACIAR projects in the region the highly effective AAHL FMD capacity development and implementation approach is regarded at the “Gold Standard” in relation to the investigation and control of FMD is SEA The project has been publicised through a press releases in Australia and articles in newsletters including the SEAFMD newsletter and on the internet The results from the project have been presented at:

of Vietnam was hampered by the unforeseen reprioritisation of DAH personnel to deal with outbreaks of AI and PRRSV

Nevertheless, the management and implementation of highly effective measures for the diagnosis and control of FMD was particularly successful in the south of Vietnam Overall, trainees were supplied in a timely manner by DAH and both the coordination and implementation of field work improved throughout the course of the project with all but one province participating The efforts made by the AAHL Scientific Coordinator in relation to mentoring and nurturing of the enthusiastic and talented personnel in the regional laboratories have led to much greater integration, cohesion and determination

The lead Vietnamese collaborating laboratory [RAHO-6] has been a driving force behind the greater “lab-to-field” and “field-to-lab” integration and the subsequent successful implementation of highly effective FMD control in the field in Vietnam In addition, as an FMD Reference Laboratory, RAHO-6 continues to conduct FMD training activities and to both support and supply diagnostic reagents to other regional laboratories

6 Report on Cross-Cutting Issues

6.1 Environment

No adverse environmental impacts associated with project activities were identified

In a wider context, it is anticipated that pig and cattle farming will become more efficient through a reduction in animal deaths and FMD disease outbreaks with resultant environmental benefit through more efficient utilization of resources

6.2 Gender and Social Issues

The establishment and successful implementation of new FMD diagnostic tests has improved the ability of regional and provincial diagnostic laboratories to quickly and accurately assess FMD outbreaks, thereby enabling rapid and appropriate control measures to be implemented in the field Significant benefits have already been observed and will continue to accrue during subsequent rounds of sero-surveillance, field virus characterisation with resultant evidence-based selection and vaccine intervention The beneficiaries of this project will be both large and small farms and particularly smallholder farmers whose animals and incomes will be protected by better disease diagnosis, management and control From a gender perspective, since women at the village level are the primary animal handlers and managers they will be major beneficiaries

7 Implementation & Sustainability Issues

7.1 Issues and Constraints

Throughout the course of this project field staff and DAH personnel were regularly diverted to investigate and control AI & PRRS outbreaks in Vietnam This significantly reduced the DAH and field staff time available for the project, led to incomplete data / sample acquisition and resulted in limited sero-surveillance in Northern and Central provinces

Initial project implementation in the field highlighted the need for further training of field veterinarians in disease investigation techniques with a focus on sample and data collection Although this was achieved in some areas, with sample and data collection improving throughout the course of the project, in some regions this issue still remains problematic and an impediment to the successful implementation of project objectives at a national level As a result of this project, there is a much greater appreciation and acknowledgement by all collaborators of the need for large scale training of field veterinarians and concomitant resources to ensure better sample collection, laboratory submission and data acquisition to facilitate the control

of FMD in Vietnam Training of DAH staff in epidemiology and data analysis was also identified as an area that will require future investment This could be achieved though greater synergy between the various aid agencies operating in Vietnam

related personnel with general disease investigation skills rather than focussing solely on HPAI

7.3 Sustainability

The DAH laboratories appear to be well supported by the central government The DAH laboratories are also receiving increased support from overseas funding agencies as part of the AI campaign to improve laboratory facilities

RAHO-6 has gained confidence as an FMD Reference Laboratory and has implemented highly successful technology transfer and training activities to other regional laboratories Of particular significance, RAHO-6 has acted as an exemplar

to other regional laboratories, and the wider animal health network in Vietnam, with regard to the successful design and implementation of highly effective FMD diagnostic, surveillance and control strategies in the field

DAH scientific staff were quick to understand new information, energetic in its implementation and demonstrated an obvious desire to do their jobs well This was particularly evident by the drive, determination and professionalism exhibited by the RAHO-6 staff in relation to the successful achievement of project objectives

This project followed the tried and tested AAHL approach for successful technology transfer and capacity development This approach began with demonstration and training at AAHL followed by the supply of reference reagents and procedures for implementation by the trainees at their own initiative in the recipient laboratory This was subsequently consolidated following a series of ongoing consultancy visits by the AAHL Scientific Coordinator to the recipient laboratory for on-site support, encouragement, appraisal and “trouble shooting”

Resultant quality assured FMD capacity development, in particular at RAHO-6 and NCVD, has proven to be highly sustainable with both laboratories having achieved FMD Reference Laboratory status in Vietnam For example, the fact that RAHO-6 is producing reference antigen for in-house use and also supplying this to other regional laboratories clearly attests to current capacity, capabilities and future sustainability In addition, RAHO-6 have, at their own initiative and unassisted by AAHL, conducted FMD training courses and technology transfer to other labs in Vietnam This project is just one of a series of highly successful AusAID and ACIAR funded projects that have linked AAHL to both Vietnamese and SEA laboratories, all

of which have been proven to be sustainable

8 Next Critical Steps

Project objectives were achieved throughout Vietnam and with considerable success

in southern Vietnam However, throughout the implementation of this project one of the key lessons learnt was in relation to the importance of complete and accurate field information and timely laboratory submission of appropriate clinical specimens Whereas laboratory scientists were accustomed to accurate recording of results, in the field the recording of information such as comprehensive vaccination records and related data was not common place

Through the implementation of a series of data collection and field surveillance forms, data collection, management and laboratory submissions have improved dramatically throughout the course of this project Nevertheless, it was clearly evident that continued training and support of field veterinarians and the greater integration and coordination of “lab-to-field” and “field-to-lab” activities will be critical for the successful implementation of FMD control measures at a national level

9 Conclusions

This project has realised objectives in relation to FMD diagnosis, surveillance and control via successful capacity development and subsequent implementation of quality assured FMD diagnostics at both a regional and a national level in Vietnam Specifically, both the RAHO-6 and NCVD laboratories have attained FMD Reference Laboratory status and possess comprehensive quality assured FMD diagnostic capabilities and the ability to isolate, serotype and genotype FMDV field isolates On

a national level, RAHO-4, RAHO-7 and NAVETCO also have the capability to perform ELISAs for the detection of FMD antigen and antibodies, further consolidating FMD diagnostic capabilities within the wider animal health network in Vietnam This project has significantly aided DAH gain a greater understanding and appreciation of the logistic requirements and field skills that will be required for the effective control of FMD in Vietnam In addition, this project has also generated important baseline data in relation to vaccination coverage in the field and the prevalence of FMD serotypes in circulation in Vietnam

In relation to FMD surveillance, resultant capabilities for serology by ELISA has enabled post-vaccination surveillance and has facilitated the investigation of the efficacy of vaccine coverage and ongoing surveillance which has demonstrated continual improvement in relation to FMD vaccine coverage in the field This is of particular significance insofar as FMD vaccine is expensive and represents the main cost component associated with FMD control in Vietnam This project has highlighted the requirement for ongoing post-vaccination sero-surveillance to achieve a greater return on investment and ensure cost effective implementation of FMD vaccination in the field in Vietnam

Of particular significance, in relation to FMD control, the subsequent serotyping and genotyping of FMDV field isolates has enabled evidence-based assessment of the prevalence and risks associated with individual FMD serotypes in specific regions This has resulted in highly informed science-based recommendations being made to Department of Animal Health (DAH), field veterinarians and farmers with regard to the selection of the most appropriate FMD vaccine serotype formulations to be used

in the field The effectiveness of this approach was clearly evident from the fact that

in southern Vietnam the incidence of FMD outbreaks has fallen continuously during the course of the project with only one isolated outbreak being reported in the 2009-

2010 reporting period

Throughout the course of this project both the quality of field data and the integrity of specimen submissions showed continuous improvement However it was widely recognised at an early stage that the critical component for FMD control was the requirement for closer integration, support and communication between laboratory and field personnel In areas where this critical issue was addressed, the effectiveness of the implementation strategy adopted was readily apparent For example, the implementation strategy at RAHO-6 where successful capability development and quality assurance in the laboratory was the driving force behind more effective training and education of field veterinarians realised project objectives and resulted in effective FMD management in the field Specifically, the two-way communication, support and collaboration between the RAHO-6 laboratory / office and field veterinarians facilitated highly effective disease diagnosis, surveillance and control in southern Vietnam The RAHO-6 epidemiology section have been highly proactive in engaging and training both provincial veterinarians and field staff and it

is considered likely that this highly successful approach will exhibit similar utility when implemented elsewhere in Vietnam once sufficient resources are made available and training of field personnel is accomplished

From an Australian perspective, this project has been highly successful, enabling AAHL personnel to gain hands-on experience in dealing with FMD diagnostics and outbreak control Of particular significance, this project also achieved further confirmation of the effectiveness of AAHL FMD diagnostic tests and staff capabilities

in relation to FMD preparedness in Australia

Acknowledgements

The project would like to acknowledge all those who participated in the project

RAHO – 6 HCMC:

Dr Dong Manh Hoa, Dr Ngo Thanh Long, Dr Nguyen Tru,

Dr Pham Phong Vu, Dr Ly Hoai Vu, Nguyen Thanh Phuong &

Dr Nguyen Thanh Phong

HANOI:

Dr Nguyen Van Cam, Dr Nguyen Thu Ha, Dr Nguyen Tung,

Dr Ngo Thu Huong, Dr Mai Thuy Duong & Dr Do Thi Hoa

SEAFMD, GF TADs, AusAID, other projects

10 Statutory Declaration

STATUTORY DECLARATION

PROGRAM

CARD Project Title: FMD - Improved surveillance capabilities for FMDV

CARD Project Number: 072/04VIE

We the undersigned hereby declare that during the project we have delivered the following inputs to assist in implementation of the above project

Vietnamese Personnel

Dr.Hoang Van Nam

120 Dr.Tran Ngoc Thang

90

Ngo Thanh Long, DVM

150 Nguyen Truc Ha, Ms

120 Pham Phong Vu, DVM

150 Nguyen Ngo M Triet, DVM

120 Tran Quoc Phong, DVM

120

Nguyen Thu Hong, Ms

120 Nguyen Van Hung, Bc

120