Executive Summary The main focus of this reporting period was to commence the project, begin the training of the field veterinarians in the South, Centre and North of Vietnam and also t
Trang 1Disease in Cattle and Pigs
Date 30/06/2006
Trang 26 Report on Cross-Cutting Issues _ 8
6.1 Environment _ 8 6.2 Gender and Social Issues _ 8
7 Implementation & Sustainability Issues _ 9
7.1 Issues and Constraints _ 9 7.2 Options 9 7.3 Sustainability _ 9
8 Next Critical Steps _ 10
9 Conclusion _ 10
10 Statuatory Declaration 13
Trang 31 Institute Information
Project Name
Vietnamese Institution Regional Animal Health Centre, Ho
Chi Minh City (RAHC-HCMC), South Vietnam
Vietnamese Project Team Leader Dr Dong Manh Hoa
Australian Organisation Australian Animal Health
Laboratory (AAHL), PMB 24, Geelong, 3213,
Australia
Australian Personnel Mr Chris Morrissy
Completion date (original) 01/06/2008
Completion date (revised)
Reporting period 31/12/2005 – 30/06/2006
Contact Officer(s)
In Australia: Team Leader
Name: Mr Chris Morrissy Telephone: +61 3 5227 5000
Position: Diagnostic Virologist
Supervisor Mammalian Virology
Fax: +61 3 5227 5555
Organisation Australian Animal Health
Laboratory (AAHL), PMB
24, Geelong, 3213, Australia
Email: chris.morrissy@csiro.au
In Australia: Administrative contact
Name: Mr Chris Morrissy Telephone: +61 3 5227 5000
Position: Patents Contracts Officer Fax: +61 3 5227 5555
Organisation Australian Animal Health
Laboratory (AAHL), PMB
24, Geelong, 3213, Australia
Email: chris.morrissy@csiro.au
In Vietnam
Name: Dr Dong Manh Hoa Telephone: + 84 8 8568220
Position: Director Fax: + 84 8 8569050
Organisation Regional Animal Health
Centre, Ho Chi Minh City (RAHC-HCMC), South Vietnam
Email: rahchcmc@hcm.vnn.vn
Trang 42 Project Abstract
The projects purpose is to determine why there are vaccine failures, and to
investigate what serotypes of FMDV are circulating in Vietnam so that the correct vaccines can be used for these serotypes Regional laboratories are setup with the reagents and methods to allow a diagnostic capability for FMDV diagnosis and serology Control strategies for understanding of FMD epidemiology have been implemented through veterinary and laboratory training workshops and laboratory testing for both virus typing and serosurveillance has begun The project has
highlighted the importance of having a laboratory network to identify what is
happening in the field and how to prevent and control disease outbreaks The pilot zones are established in provinces near the borders of Vietnam to study serotypes circulating in Vietnam and to determine their origin The quality and collection of samples have increased giving more data on the FMD situation in Vietnam
Molecular epidemiological studies of the FMDV isolates in these provinces will
provide insights to the effectiveness of border control and the origin of FMDV
circulating in Vietnam each year A diagnostic capacity for FMD would allow the early detection and identification of disease enabling better control of disease and help reduce loss of livestock and so productivity
3 Executive Summary
The main focus of this reporting period was to commence the project, begin the training of the field veterinarians in the South, Centre and North of Vietnam and also the training of laboratory staff All laboratory equipment was purchased and supplied
to the laboratories in Vietnam All laboratory consumables and reagents were also supplied to the laboratories in Vietnam for the first 6 months of the project AAHL staff prepared the information necessary for the training of field veterinarians This workshop information and the lectures were used to run workshops for the field veterinarians in the South and Centre of Vietnam and this information was then transferred to Dr Hoa and the staff at Ho Chi Minh City Regional Laboratory who gave the third workshop in Hanoi The field studies were planned and implemented through the veterinary workshops, the field studies began with the first sero-
surveillance survey in the South and Centre of Vietnam and the collection of
outbreak samples The Regional Animal Health Centre in Ho Chi Minh City also visited each province to train the field veterinarians on how to collect samples and how to collect information necessary for the history of the animals on the villages in the project One staff member from the Regional Animal Health laboratory and one staff member from NAVETCO attended AAHL for training in ELISA technology for the diagnosis of FMD and standardisation of the reagents to be used in the first year
of the project Two AAHL staff consultants carried out workshops for the field
veterinarians in the South and Centre of Vietnam The third veterinary workshop in North Vietnam was carried out by DAH staff Two AAHL consultants also carried out
a workshop for 20 participants from each of the laboratories in the project and the regional laboratories in South Vietnam, in ELISA technologies for diagnosis of FMD
at the Regional Animal Health Centre in Ho Chi Minh City An important part of the laboratory training was Quality Assurance in the laboratory to ensure tests will be run according to a standard protocol and to allow AAHL to audit the results from each laboratory and give the appropriate data to allow trouble shooting Avian
Influenza (AI) has extended the sero-surveillance in the first six months
Trang 54 Introduction & Background
Objectives of the project:
1 To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance
2 To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies
Completing these objectives will improve the diagnostic capability of the
Veterinary laboratories in Vietnam and the training of DAH veterinarians in disease investigation and control This will strengthen the profile of DAH which will play a vital role in making Vietnam more economically competitive Improved animal health will lead to an increase in rural productivity though increased animal production and indirectly in increased crop production Healthy animals will enable small farmers to be more competitive in the local market Control of FMD and animal diseases in general will give poor farmers a more stable
income stream and reduce their vulnerability to natural and economic problems Establishing a diagnostic network which extends from the North to South
Vietnam, from the laboratory to the farm level, reinforced by training and
education, will give Vietnam a working model on which to base disease control This will directly increase the competitiveness and productivity of the national agricultural system which includes the major areas of concern including the Mekong Delta and the Central Coast
Implementation Approach and Strategy
The project approach is thought to be the most appropriate for developing an understanding of FMD epidemiology in Vietnam The approach for technology transfer is well established at AAHL and has been successfully applied in
previous projects in Vietnam, Thailand and Indonesia The field studies and epidemiology and serosurveillance approaches have been designed and
planned in conjunction with DAH to provide the maximum necessary information
to demonstrate the FMD situation in Vietnam and the effectiveness of FMD vaccines The diagnostic technologies that will be used in this approach are the standard diagnostic tests in use throughout the world to study FMD as directed
by OIE
AAHL has a lot of experience with field surveys for prevalence of antibodies, as
in the ACIAR projects in Laos and Thailand on FMD The Philippines is another example where OIE standard diagnostic tests are being used to control and eradicate FMD
Trang 65 Progress to Date
5.1 Implementation Highlights
Main achievements for this reporting period were:
• Training of laboratory staff at AAHL, one representative from each laboratory were trained in ELISA techniques for the detection of FMD antigen and
antibody and the standardisation of reagents Training was carried out under a quality system emphasizing the importance of Quality Assurance in the
laboratory focusing on test maintenance, record keeping and data collection
• A consultant from AAHL completed training of HCMC-RAHC staff in cell culture techniques and established this technology there to be used to grow FMD isolates from the field The growth of FMD isolates from the field is important to allow further analysis of FMD field isolates by PCR and sequencing, Again Quality Assurance, record keeping and data collection was emphasized
• Further training of field veterinarians and feedback of the results from first round of testing and shared the experience from the first round of sample collection with the field veterinary staff to increase the quality of collected
• Sampling for second phase of sera collection completed for each province for epidemiological studies and serosurveillance The collection of tissue samples from outbreaks continued to increase giving valuable information on the
serotypes circulating in Vietnam
• ELISA technology in use at collaborating laboratories in HCMC, Hanoi and Can Tho, for serotyping of FMD tissue samples from the field Sera from first round
of serosurveillance tested at RAHC-HCMC
• Virus isolation in use at RAHC-HCMC Molecular technology and serology by VNT using cell culture has began to be used
• Supply of consumables and further reagents for testing of samples by FMD ELISA, molecular technology and cell culture
Trang 7Report from Vietnam:
Activities from Jan 1 st 2006 to May 30 th 2006
2 Using the above cells to isolate FMDV in Vietnam and compare the sensitivity between the cells
Ms Catherine William
Dr Long Dr.Phong Dr.Vu
2 Operation under a Quality Assurance system
3 Reagent standardisation
Mr Chris Morrissy
Ms Linda
5 participants from 4 partner laboratories (NVDC, RAHC-HCMC, CAN THO , DA NANG ) and Sub-DAH- HCMC
2 Field activities
2.1 Visit 8 provinces in the project (Middle and South Vietnam), March 2006
- Transfer the equipment and documents (form and questionnaire) for
sample collection
- Transfer money for sample collection
- Feed back the results from first testing round and share the experience
on sample collection with the field veterinary staff to increase the quality
of collected samples
- Staff in charge: Dr Hoa, Dr Vu , Dr Ha ( RAHC-HCMC)
Dr Quan, Dr.Quang (RAHC-Da Nang)
Dr Thanh, Dr Dung (RAHC-Can Tho) 2.1.2 Visit 2 provinces in the project (North Vietnam), March 2006
- Transfer the equipment and documents (form and questionnaire) for
sample collection
- Transfer money for sample collection
Trang 8- Feed back the results from first testing round and share the experience
on sample collection with the field veterinary staff to increase the quality
of collected samples
- Staff in charge: Dr Cam, Dr Tung (NVDC- Hanoi)
2.1.3 Visit 3 laboratories in the Project ( NVDC-Ha Noi , RAHC-Can tho, Da nang)
early May, 2006
- Transfer the ELISA reagents
- Discuss the plan for second testing round and share experience on
laboratory management focusing on laboratory Quality Assurance and test management
- Staff in charge: Mr Chris Morrissy, Dr Long
2.2 Collect sera samples from 10 provinces for the second round of surveillance,
120 cattle sera and 120 pig sera from each province
- NVDC-Ha Noi: 2 provinces (Quang Ninh and Lang Son), total 480 sera
- RAHC-Da Nang: 2 provinces (Kontum and Quang Nam), total 480 sera
- RAHC-Can Tho: 2 provinces (An Giang, Kien Giang ), total 480 sera
- RAHC-HCMC: 4 provinces (Binh Phuoc, Tay Ninh, Long An, Dong
Thap), total 960 sera 2.3 Collection of tissue samples from outbreaks for FMDV serotyping
Trang 9- Testing on tissue samples for FMDV serotyping : 34 samples, all of them are serotype O
- Testing on sera for FMDV antibody detection: 960 sera , on going
- Isolation FMDV by BHK cell line to confirm 3 samples from Hanoi, 1 of them is serotype Asia1 in pigs and 1 sample is serotype O and1 sample virus could not isolated
- Isolation FMDV by BHK cell line from 39 selected field samples, 1 was serotype Asia1 and 38 were serotype O
- Application of Virus Neutralisation Test (VNT) protocol (testing phase
to setup VNT technology at RAHC-HCMC):
• Virus titration for serotype O and A , two viruses for each serotype
• VNT applied using the serum from vaccine trial on cattle
- RT-PCR application (testing phase to setup molecular technology at RAHC-HCMC, May, 2006)
• Extract RNA from virus serotype O, A and Asia 1, two viruses for each serotype
• Make cDNA from these RNA
• RT-PCR carried out using primers to detect serotype O and A
• Sent cDNA and amplicons to AAHL for sequencing
Reporter: Ngo Thanh Long
Note: Further detail on activities for reporting period in log frame
5.2 Smallholder Benefits
All pig and cattle and producers are potential beneficiaries Those that take up the advice and use vaccine according to recommendations will benefit financially
through reduced losses due to death and disease in their cattle and pigs The
benefits of a more profitable farming operation flow to all family members Farmers and district veterinarians will have improved knowledge and skill in disease
prevention, knowledge on the selection of the right vaccine and improving the
efficacy of vaccination
Trang 105.3 Capacity Building
Training and education of field veterinarians in disease prevention, disease
investigation and sample collection has been carried out in the North, Centre and South of Vietnam This training has already shown an impact with an increase in quality of sample collection and number of samples collected and submitted to the laboratory The project has provided training and technology transfer of FMD
diagnostics to each laboratory involved in the project Reagents and standard
methods have been supplied to each laboratory giving them the diagnostic capability for FMDV diagnosis and serology using ELISA technology, this technology is now able to be practised at each laboratory
5.4 Publicity
The CARD AusAID project have received publicity through the training programs and also through the achievements so far in the project in understanding FMD in Vietnam FMD is a disease on importance in Vietnam and this has put our project into the lime light The project has been publicised through a press release in
Australia and articles in news letters including the SEAFMD newsletter
5.5 Project Management
Implementation of the project is on schedule AAHL has kept the project on target and has supported or requests from the Vietnamese partners Training has been carried out in Australia and Vietnam with both partners benefiting from the
information that is being generated
Implementation in Vietnam is also continuing to go well Trainees have been
supplied in a timely manner, from DAH The field work has been organised well with data and samples being collected from farms in each pilot zone Training and the transfer of information to other regional laboratories in Vietnam is also occurring RAHC-HCMC will supply support to these laboratories
6 Report on Cross-Cutting Issues
6.1 Environment
No direct environmental impacts associated with project activities have been
identified On a broader scale, the intention is that pig and cattle farming will become more efficient through a reduction in animal death and disease Environmental
benefit will result through more efficient utilization of resources such as animal feed, the energy to provide animal feed and to run other aspects of the farm
6.2 Gender and Social Issues
Application of new diagnostic tests will improve the ability of regional and provincial diagnostic units to quickly and accurately assess FMD outbreaks, enabling rapid and appropriate measures to be applied to control disease These benefits will begin during the project and continue to accrue with continued application The
beneficiaries of this project will be both large and small farms and particularly
smallholder farmers whose animals and incomes will be protected by better disease diagnosis, management and control Since women at the village level are the
primary animal handlers and managers, they will be major beneficiaries of the final outcome of better diagnosis and control of animal diseases
Trang 117 Implementation & Sustainability Issues
7.1 Issues and Constraints
DAH has been very busy controlling the AI outbreaks in Vietnam and this has made there workload increase dramatically, this have meant some delays and
rescheduling of training field veterinarians, eg training in the North Vietnam
rescheduled Sampling has also been spread out over a longer time period as well but this has had no effect with all samples still being collected The collection of samples was made more difficult by the need to train all veterinarians in each
province in data collection, on how to collect samples, collection of blood and how to restrain cattle This increased training need has highlighted the need for further training of field veterinarians throughout Vietnam in disease investigation techniques with a focus on sample collection and data collection There is a need for further input in this area, large scale training of field veterinarians to ensure better
knowledge and control of disease in Vietnam This could be achieved though better collaboration of aid agencies in Vietnam especially those supplying training for AI
7.2 Options
The government of Vietnam is looking at increasing the support to DAH and has increased funding for AI diagnosis and is looking to do the same for FMD diagnosis The increased funding for AI has seen laboratories updated with new equipment some of which will improve all disease diagnosis which includes FMD
The money available to improve AI diagnosis can improve all disease diagnosis if this money is used wisely, eg in training field veterinarians in AI diagnosis, the
training should be general to cover all diseases including FMD, CSF and other
diseases of importance in Vietnam Also in improving AI diagnosis in laboratories the training should emphasize how improvement can be applied to all areas of
diagnosis, eg quality assurance in the laboratory
7.3 Sustainability
The DAH laboratories appear to be well supported by the central government The DAH laboratories are also receiving increased support from overseas funding
agencies as part of the AI campaign to improve laboratory facilities
RAHC-HCMC is developing in confidence as an institution This confidence is
beginning to be passed on to other DAH laboratories The DAH scientific staff are quick to understand new information and are energetic in its implementation People have an obvious desire to do their jobs well This desire is obvious in staff in RAHC-HCMC and other laboratories The laboratories just need technical support to be encouraged to carry out the techniques that they need for diagnosis and research The project is following a well tried model for successful technology transfer, of demonstration and teaching of the technology in the donor facility followed by supply
of reagents for implementation by the trainees at their own initiative in the recipient laboratory, subsequently backed up by consultancy visits to the recipient laboratory for support and fine tuning A useful indicator of likely success is the initiative and ability of the trainees to make a useful attempt at implementation by themselves DAH and NAVETCO staff show a high level of involvement in this way
The AusAID funded CSFV project, that linked DAH, NaVetCo and AAHL prior to this project, is proving sustainable NAVETCO and DAH not only maintains the
diagnostic capability developed at that time, but on its own initiative and unassisted
Trang 12by AAHL is conducting training courses and technology transfer to other labs in Vietnam
8 Next Critical Steps
In the next 6 months a meeting will be held at AAHL to review the project outputs and to examine the information collected so far in the project, The meeting will
decide if the data collected from the field is giving DAH the necessary information to make discisions on FMD control An AAHL empidemilogist will be involved in
collating the data from the first 2 phases od serosurveillance and epipdemlogical data collection and begin exposing DAH staff to data analysis techniques The third phase of the sero-surveillance for the collection of the samples will be implemented with any changes necessary In the next 6 months we will finalise all of the testing of the samples collected and evaluate each laboratories capabilities In the second 6 months of the project it was decided to bring forward the training of the staff from each laboratory to be held at AAHL This training will be carried out at AAHL for 1 representative from each laboratory for training in ELISA techniques
A consultant from AAHL will complete training of the HCMC-RAHC in cell culture techniques and establish this technology there to be used to grow FMD isolates from the field The growth of FMD isolates from the field is important to allow further
analysis of these isolates by PCR and sequencing
9 Conclusion
The project activities are on schedule The DAH laboratories are running the field trials on time, even though they have had increased workload with avian influenza outbreaks It is expected that the second sero-surveillance survey will be much
easier than the first The first took a lot longer to do due to commitments with avian influenza and also understanding the problems associated with collecting samples in areas where samples had not been collected before Field veterinarians needed to
be trained in the collection of tissue samples from infected animals as well as
bleeding animals The staff at Regional Animal Health Centre, Ho Chi Min City have performed a excellent job in getting everything to run on schedule in this first six months The project should continue to run on time and I expect no further problems especially how the collection procedures are in place The staff at DAH are very eager to learn and this is making a very good environment for transferring
technology The laboratory at Da Nang has less experience and this laboratory will need much more contact with AAHL and the laboratory at RAHC-HCMC to achieve the transfer of the ELISA technology to this laboratory
The laboratory in Hanoi has the skills and is doing quite well in the application of the ELISA but this laboratory needs further contact with AAHL to increase their
understanding of the application of this technology for FMD diagnosis and this also applies to the laboratory in Can Tho in the south Can Tho is applying the
technologies learnt
This project has proved to be an important project for Vietnam with the importance of FMD and this has meant good publicity for the AusAID CARD program It has also meant an increase in requests for help in other areas of animal health with the
success of this project in improving the skills and technology at RAHC-HCMC and NAVETCO and through our other project in CSF at NAVETCO and DAH and Duck Plague at NAVETCO DAH is using this project as an example of how to approach FMD control
Trang 1310 Statutory Declaration
STATUTORY DECLARATION
COLLABORATION FOR AGRICULTURE AND RURAL DEVELOPMENT
PROGRAM
CARD Project Title: FMD - Improved surveillance capabilities for FMDV
CARD Project Number: 072/04VIE
We the undersigned hereby declare that during the period 01/06/2005 to 31/12/2005
we have delivered the following inputs to assist in implementation of the above project
1 PERSONNEL INPUTS
Australian Personnel
Provided (Name)
Days in Vietnam
Days in Australia
Trips to Vietnam
Chris Morrissy (also
replaced Laurie Gleeson’s
time in project)
28 10 2
Lynda Wright (replaced
Brenda Van Der Heide in
RAHC-HCMC
NVDC-HA NOI
Trang 14NAVETCO
2 EQUIPMENT AND OTHER SERVICES
Equipment & Other Services