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2011 WASTEWATER TREATMENT & REUSE Introduction to microbial metabolism CONTENTS Carbon and energy sources for microbial growth Nutrient and growth factor requirements IS IT IMPORTANT?. B

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SPECIALIZED ENGLISH – SEMESTER 5

DEPARTMENT OF ENVIRONMENTAL TECHNOLOGY AND

MANAGEMENT

Chapter 23 FUNDAMENTALS OF BIOLOGICAL

Dr Tran Thi My Dieu

Sep 2011

WASTEWATER TREATMENT & REUSE

Introduction to microbial metabolism

CONTENTS

Carbon and energy sources for microbial growth

Nutrient and growth factor requirements

IS IT IMPORTANT?

Basic to design or to select type of

biological process is understanding of

the biochemical activities

Different microorganism use a wide

range of electron acceptors

CARBON AND ENERGY SOURCES FOR

MICROBIAL GROWTH

To continue to reproduce and function

properly

Energy and carbon

Inorganic elements

Substrates

nutrients and trace elements Growth factor (organic nutrients)

CARBON AND ENERGY SOURCES FOR

MICROBIAL GROWTH

Synthesis Nutrients

New cells

Organic

Energy

O 2

New cells

CO 2 + H 2 O

Bacteria metabolism: aerobic, heterotrophic

CARBON AND ENERGY SOURCES FOR

MICROBIAL GROWTH

Synthesis Nutrients

Bacteria metabolism: anaerobic, heterotrophic

Organic compounds

Synthesis Bacteria Energy

New cells

CH 4 & CO 2

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CARBON AND ENERGY SOURCES FOR

MICROBIAL GROWTH

Nutrients

New cells

Bacteria metabolism: aerobic, autotrophic

2

Bacteria Energy

O 2

New cells

NO 2 or NO 3

NH 4 +

CARBON AND ENERGY SOURCES FOR

MICROBIAL GROWTH

Carbon sources?

Organic matter Carbon dioxide

Organisms use organic carbon for the formation of new

Organisms derive cell

autotrophs

CARBON AND ENERGY SOURCES FOR

MICROBIAL GROWTH

Energy sources?

Supplied by light By a chemical

oxidation reaction

Organisms use light as

an energy source =

phototrophs

Organisms derive their energy from chemical reactions = chemotrophs

CARBON AND ENERGY SOURCES FOR

MICROBIAL GROWTH

Energy sources?

Heterotrophic

Phototrophic organisms

reducing bacteria) Autotrophic (alage &

photosynthetic bacteria)

CARBON AND ENERGY SOURCES FOR

MICROBIAL GROWTH

Energy sources?

Heterotrophic (protozoa, fungi

Phototrophs

Chemotrophs

& most bacteria) Autotrophic (nitrifying bacteria)

CARBON AND ENERGY SOURCES FOR

MICROBIAL GROWTH

Energy sources?

Obtain energy from the oxidation of reduced inorganic

-,

Fe 2+ , S

2-Derive energy from oxidation of organic compounds

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NUTRIENT AND GROWTH FACTOR

REQUIREMENTS

The principal inorganic nutrients needed?

N S P K Mg Ca Fe Na and Cl

N, S, P, K, Mg, Ca, Fe, Na and Cl

Minor nutrients of importance?

Zn, Mn, Mo, Se, Co, Cu and Ni

NUTRIENT AND GROWTH FACTOR

REQUIREMENTS

Growth factors

(1) Amino acid

(1) Amino acid

(2) Nitrogen bases

(3) Vitamins

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