Collaboration for Agriculture & Rural Development:" Field evaluation and advanced vegetative mass-propagation technology for scaling up high-value plantations of Pinus caribaea and related hybrids in Vietnam - Milestone 4" pptx

Ministry of Agriculture & Rural Development Collaboration for Agriculture & Rural Development Project 033/05 VIE Field evaluation and advanced vegetative mass-propagation technology fo

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Ministry of Agriculture & Rural Development

Collaboration for Agriculture & Rural Development

Project 033/05 VIE

Field evaluation and advanced vegetative mass-propagation technology for scaling

up high-value plantations of Pinus caribaea and related hybrids in Vietnam

Milestone 4:

Output 1.2: Genetic Evaluation Trials

Deliverables: Means of verification for payment

• At least 5 genetic evaluation trials sites established to compare performance of locally available and introduced pines

• Trial protocol documented and responsibilities for long term management and research evaluation analysis assigned

Report Title:

Commencement report for trials established in 2006 to compare performance of local and introduced tropical pines in Vietnam

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INTRODUCTION

CARD Project 033/VIE seeks to review existing experience with and performance of several tropical conifer species in Vietnam, particularly Caribbean Pine, and to establish further trials of improved genetic material, including hybrids

The project will also provide capacity building for Vietnamese forest research institutions

relating to Pinus tree improvement and mass propagation systems via training in Queensland and

Vietnam, development of small-scale hedge and nursery infrastructure, and a study tour to Australia, involving innovative Vietnamese forest managers and researchers

Finally, the project will build awareness among large and small-scale forest growers including ethnic minorities, through the establishment of collaborative demonstration plantings in priority

regions for Pinus plantation expansion, supported by appropriate training

This report relates to Output 1.2 of the Project: “Establish genetic evaluation trials to compare

performance of locally available and introduced pines (especially Pinus caribaea and hybrids)

on multiple sites throughout North Central, Central Coast and Central Highlands regions of Vietnam

PROGRESS

Description of Sites

Table 1 describes key features of six sites where genetic evaluation trials have been established

A seventh site near Dong Ha on the Central Coast is proposed for planting however

arrangements have not yet been finalised

Photos for some of the sites are included in Appendix 3

Genetic Material Treatments

Table 2 lists the range of genetic material treatments used at each site Generally speaking,

plants for the trials were raised at the RCFTI Nursery in Cam Quy, Ba Vi for sites in northern Vietnam and at the Cam Ly Nursery, Lam Dong Forest Research Centre for the Central

Highlands sites

Additional local treatments were sourced from RCFTI (local Pinus caribaea var hondurensis) and from local nurseries (Pinus merkussii)

Experimental design and layout

Generally speaking, the trials were laid out as 6 row x 6 tree (36 tree) plots for each treatment,

arranged as a Randomised Complete Block (RCB) design with 4 replications Appendix 1

includes a site map for each site

Early survival, early growth and re-filling

Following early losses, some sites were re-filled with plants within the first couple of months of planting In addition, some sites have recently been assessed for survival and early age height growth Results will be reported at a later stage

Protocols for trial management, data collection, analysis and reporting

The agency responsible for overall co-ordination of the trials is the Research Centre for Forest Tree Improvement (RCFTI) based in Hanoi The nominated contact person is:

Name: Mr Phi Hong Hai

Position title: Deputy-director of RCFTI

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Contact Details: Phone No 844 913309746/ 844 8389813

Email: phi.hong.hai@fsiv.org.vn

Responsibilities for this position include:

• Subject to availability of funding, co-ordinate trial assessments, based broadly on the

schedule listed below:

(a) Year 1: Survival, Height (cm)

(b) Year 3: Survival, Height (cm), Diameter at breast height over bark (DBHOB)

(c) Year 5: Survival, Height (dm), DBHOB (cm), Tree form & health assessment

(d) Year 10: Survival, Height (dm), DBHOB (cm), Tree form & health assessment

• Enter data into an electronic database for secure storage

• Co-ordinate data analysis and reporting using existing resources Australian partners may be able to assist

• Distribute results and reports to site managers and Australian Team Leader

Appendix 2 lists the names of local site managers responsible for management of the relevant

trial site Responsibilities include:

• Protect trials from damaging or competing agents such as over-topping weeds, animal browsing, fire and firewood collection;

• Monitor health of trials and report any issues of interest (eg pest or insect damage, wildfire, general decline in tree health, interference by other forest users); and

• Provide local assistance to assess trials as requested by RCFTI Co-ordinator

FUTURE DIRECTIONS

Future work required for this series of trials includes:

• Plant remaining site at Dong Ha when weather conditions are suitable

• Update this report to include Dong Ha details when confirmed

• Collate and report early age growth and survival data for the various trials

• Continue to maintain trial sites free from competing weeds and other interference

• Periodic assessment and reporting as per responsibilities outlined in this document

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Table 1: Description of Pinus genetic evaluation sites planted in 2006

Forest Enterprise, part of Vinapaco

Phu Ninh FRC Lam Dong FRC Gia Lai Tropical

Forestry Centre

Non-timber Forest Research Centre

Elevation

(m)

Annual

Rainfall

1,680 1,387 1,500 1,730 2,270 2,061

at 50cm

Reasonable, yellow medium clay

Yellow medium clay + quartz outcrops

Deep, red, well-drained

Ferralit, medium to deep soil layer, red – yellow

Cultivation Ripping to 70cm +

40cmx40cmx40cm manual pit

cultivation

cultivation Fertiliser mixed in

cultivation

Fertiliser 200gm

superphosphate/tree

200gm superphosphate/tree

Tree spacing 3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha)

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Table 2: Genetic treatments included in 2006 plant Pinus genetic evaluation trials: Number = treatment number for particular trial

P caribaea var hondurensis (PCH) – non select – Dai Lai 3 3 3 8 8 9

P caribaea var hondurensis (PCH) –select – elite seed

from 11 plus trees, Dai Lai SPA

P caribaea var hondurensis (PCH) – non select –

Queensland

P caribaea var hondurensis (PCH) –select – Queensland 6 6 N/A 5 5 7

P caribaea var hondurensis (PCH) –Cam Quy –

non-select

7

P caribaea var hondurensis (PCH) – FRC source N/A 7 N/A N/A N/A N/A

P elliottii var elliottii x PCH = PEE x PCH F2 hybrid -

Queensland

PCH x P caribaea var bahamensis (PCH x PCB) -

Queensland

PCH x P caribaea var caribaea (PCH x PCC) –

Queensland

PEE x PCH Series II clones

(a) clone c3640, bare root cuttings

(b) clone c 3136, bare root cuttings

(c) clone c 4124, bare root cuttings

11 11a 11c 11b

11 11a 11b 11c

10a 10b (11)

N/A = Not Applicable to this site

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Appendix 1: Site Design maps for CARD Pinus genetic evaluation trials established in 2006

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SITE 1: Experimental design for Pinus caribaea & hybrid pine

at Cam Quy, Ba Vi – Ha Tay (Vietnam)

(Part of CARD 033/05 VIE Project)

+ Date of planting: August 2006

+ Trial design: RCB with 6 rows and 6 columns

+ Treatments: 11

+ Replications: 4

+ Trees/ plot: 36

+ Planted spacing: 3 x 3 m

+ Hole: 40 x 40 x 40 cm

+ Fertilizer: 200 gram Super Phosphate/ plot

+ Total area: 2 ha(experimental area - 1.5 ha)

DT1

Dai Lai DT2

(no select) QLQ

(non select) 4:mass

10.c 15.c 34.c

11

6 5 1 2 9

c a b

10 8 3 7 4

11

8 7 3 5 4 1 9 2 6 10

b c a

11

3 2 4 10 8 6 7

b a c

9 5 1

11

1 5 9

a b c

7 2 6 10 4 8 3

Outside border, 1 row, 4 P.massoniana

Acacia hybrid

Note:

P.caribaea var bahamensis

PCH x PCB Queensland

P.caribaea var caribaea

PCH x PCC Queensland

11a= C4124, 11b= C3640, 11c= C3136

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SITE 2: Experimental design for Pinus caribaea and hybrid pine

at Lap Thach – Vinh Phuc (Vietnam)

5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5

x x x a b c x x x x x x x x x x x x x x x x x x x x x x x x x x x x 3 3 3

x x x a b c x x x x x x x x x x x x x x x x x x x x x x x x x x x x 3 3 3

x x x a b c x x x x x x x x x x x x x x x x x x x x x x x x x x x x 3 3

x x x a b c x x x x x x x x x x x x x x x x x x x x x x x x x x x x 3 3

x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x b c a 3 3

x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x b c a 3

x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x b c a 3

x x x x x x a c b x x x x x x x x x x x x x x x x x x x x x x x x x 3

x x x x x x a c b x x x x x x x x x x x x x x x x x x x x x x x x x 3

1 x x x x x x a c b x x x x x x x x x x x x x x x x x x x x x x x x 3

1 x x x x x x x x x x x x x x x c b a x x x x x x x x x x x x x x x 3

1 x x x x x x x x x x x x x x x x c b a x x x x x x x x x x x x x x x 3

1 1 x x x x x x x x x x x x x x x c b a x x x x x x x x x x x x x x x 3

1 1 x x x x x x x x x x x x x x x x c b a x x x x x x x x x x x x x x x 3

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3

1 1 1 1 1

1 1

1 3 5 7 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68

1 Pinus massoniana - PMASS

X 3m X X X X X 2 Pinus caribaea var caribaea - PCC ( Thõa Thiªn HuÕ)

3m 3 PCH (no select) Dai Lai

X X X X X X 4 PCH (select) Dai Lai

5 PCH (no select) Queensland

X X X X X X 6 PCH (select) Queensland

15m 7 PCH - FRC source

X X X X X X 8 PEE x PCH F2 (Queensland)

9 PCH x PCB (Queensland) C3640

X X X X X X 10 PCH x PCC (Queensland)

11 PEE x PCH cutting (Queensland) C3136

X X X X X X

C4124

Experimental design for Pinus caribaea and hybrid pine

at Ngoc My - Lap Thach Fores try Enterpris e - Phu Tho

Date planted: 29 July 2006

(As a part of CARD 033/05 VIE Project)

North direction

(b) (c)

Xuan Hoa vil 12 km

Highway 2C 4 km

15m

Road

(a)

Rep I

Rep II

Rep III

Rep IV

11 1

7 9 6 3 10 5

2 8 4

7 2 5 8

6 9 3 10 1

4 11

Terrace Field of Cassava

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at Dai Lai – Vinh Phuc (Vietnam)

+ Date of planted: 31 August 2006

+ Design as randomized complete block with 2 rows and 4 columns

+ Treatments: 8

+ Replications: 4

+ Trees/ plot: 36

+ Total area: 1.02 ha (with experimental area: 0.54 ha)

7 3

1 8

6 2

5 4

7 6

5

8

1

3

4

2

7 4

3 5

2

8

6

7

1

4

6

2

5

3

8

1

Rep 4

♣ ♣ ♣ ♣ ♣ ♣

♣ ♣ ♣ ♣ ♣ ♣

Rep 2

Rep 1

1 edge row

P mass TDao

5 rows (4) PCC-Hue

4 rows (3)

P mass- TDao

1 edge row

P mass TDao

1 edge row

P mass TDao

1 edge row

P mass TDao

1 edge row

P mass TDao

1 edge row

P mass TDao

PCC-Hue

(4)

PCH (No select)

Dai Lai (1)

P mass

T Dao (3)

PCH (no select)

Treatments:

In genetic trial

1 P massoniana Tam Dao

2 PCC – Hue

3 PCH (no select) Dai Lai

4 PCH (select) Dai Lai

5 PCH (no select) QLD

6 PCB Cam Quy (Ha Tay)

7 PEE x PCH (F2) QLD

8 PCH x PCC (F1) QLD (1 8 is tree code in layout)

On buffer zone

1 PCH (no select) Dai Lai

2 PCH (no select) QLD

3 P.Mass Tam Dao

4 PCC Hue (Note: plot 4 & 5 of Rep.4 destroyed by illegal harvest by local people and re-planted

with A mangium)

Rep 3

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SITE 4: EXPERIMENTAL DESIGN FOR PINUS CARIBAEA AND HYBRID PINE AT

LANG HANH – LAM DONG

(Date planted: July 2006)

1 2 3 4 5 6

13 14 15 16 17 18

19 20 21 22 23 24

25 26 27 28 29 30

31 32 33 34 35 36

36 trees/plot

Road among plots 5m

Rep 4

Road 5m

Treatments:

1 P.kesiya (no select) Da Lat (PKES)

2 P.caribaea var caribaea – Hue (PCC)

3 P.caribaea var hondurensis (select) Dai Lai

4 P.caribaea var hondurensis (no select) Queensland

5 P.caribaea var hondurensis (select) Queensland

6 P.caribaea var hondurensis x P.caribaea var caribaea – Queensland (PCH x PCC)

7 P.elliottii var elliottii x PCH F2 Queensland (PEE x PCH)

8 P.caribaea var hondurensis (no select) Dai Lai (PCH)

9 P.kesiya (select) Da Lat

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SITE 5: EXPERIMENTAL DESIGN FOR PINUS CARIBAEA AND HYBRID PINE

AT GIA LAI - PLEIKU

(Date of planted: July 2006)

P.kesiya trial planted 1986

1 2 3 4 5 6

13 14 15 16 17 18

19 20 21 22 23 24

25 26 27 28 29 30

31 32 33 34 35 36

36 trees/plot

Fence

Treatments:

1 P.kesiya (no select) Da Lat (PKES)

2 P.caribaea var caribaea – Hue (PCC)

3 P.caribaea var hondurensis (select) Dai Lai

4 P.caribaea var hondurensis (no select) Queensland

5 P.caribaea var hondurensis (select) Queensland

6 P.caribaea var hondurensis x P.caribaea var caribaea – Queensland (PCH x PCC)

7 P.elliottii var elliottii x PCH F2 Queensland (PEE x PCH)

8 P.caribaea var hondurensis (no select) Dai Lai (PCH)

9 P.kesiya (select) Da Lat

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at Hoanh Bo – Quang Ninh (Vietnam)

+ Date planted: 24 July 2006

+ Design as randomized complete block with 2 rows and 5 columns

+ Treatments: 10

+ Replications: 4

+ Trees/ plot: 36

+ Total area: 2 ha (with experimental area: 1.35 ha)

+ Planted spacing: 3 x 3 m

+ Hole: 40 x 40 x 40 cm

+ Fertilizer: 200 gram Super Phosphate/ plot

P mas TD

10 8 1 3 7

PCH DL

No -select

PCH Qld No-select

PCC Hue

Rep 4

Rep 3

Rep 2

Rep 1

Top of

hill

Road

Macadamia

Botanic Garden

Treatments:

♣ ♣ ♣ ♣ ♣ ♣

♣ ♣ ♣ ♣ ♣ ♣

P massoniana

Tam Dao Macadamia

1 P massoniana Tam Dao

2 PCH x PCC Queensland

3 PCH x PCB Queensland

4 PCB Queensland vµ PCC Hue

5 PEE x PCH (F2)

6 PCH Qld (No-select)

7 PCH Qld (Select)

8 PCH Dai Lai (Select)

9 PCH Dai Lai (No-select)

10 PEE x PCH (F1)-clonal cuttings includes C3640: 60 cuttings; C4124: 54 cuttings (1 10 is code that shown in above experimental trial)

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