(Tiểu luận) development of a quechers based methodcombined with gas chromatographymass spectrometry for the analysis ofalkanes in sediments

SAMPLE PREPARATION TECHNIQUES INANALYTICAL CHEMISTRY GROUP 6 Development of a QuEChERS-based method combined with gas chromatography mass spectrometry for the analysis of alkanes in sedi

SAMPLE PREPARATION TECHNIQUES IN

ANALYTICAL CHEMISTRY

GROUP 6

Development of a QuEChERS-based method

combined with gas chromatography mass

spectrometry for the analysis of alkanes in

sediments

Lecturer: PhD Tran Thi Thanh Thuy

MEMBER ASSIGNMENTS

Pham Thi Thu Thao 20002021 Prepare Introduction, edit ppt

Le Tan Bao 20001931 Prepare Scope of application

Ho Ngoc Kieu 20005051 Prepare Meaning of method, present Tran Thi Hong Phuc 20005541 Prepare Principle of method

Ho Hong Phuc 20001841 Prepare QuEChERS Extraction, present Tran Anh Tu 20035401 Summarize contents, edit ppt

2 APPLICATION

METHOD

4

QuEChERS

5 EXTRACTION

PRINCIPLE OF METHOD

INTRODUCTION

QuEChERS

Qu ick

✓Simple

E asy

Ch eap

✓Effective

E ffective

Rugged

✓High quality

S afe results

• Was introduced in 2002

• A type of dispersive solid phase extraction (dSPE) used for sample preparation

• Determine 29n – alkanes (C12 – C40) and two isoprenoid acyclic hydrocarbons (pristane and phytane) in sediments

APPLICATION

Extract alkanes from C 22 to C 34

with an odd to even predominance,

highest in C 31 This is consistent

with a vascular land plant input.

These results can be used for

further paleoclimate investigations.

Lady-fern from Vascular plants

MEANING OF METHOD

Develop a method:

✓ Low cost

✓ Short analytical time

✓ Reduction of solvent consumption and possible associated contamination

✓ Avoid the need for sophisticated devices

✓ Diminish possible auto-oxidation reactions

Alkane extraction by QuEChERS method would be more in line with the principles of Green Analytical Chemistry

PRINCIPLE OF METHOD

Partitioning dSPE clean up

ACN

LC-MS

• The analyte in the sample is extracted in a suitable solvent

• The analyte in the extract is cleaned with an impurity adsorption

QuEChERS

EXTRACTION

SAMPLE SELECTION

Samples were collected from archaeological sites in Austria and Spain

Samples is described as homogeneous beige silt, approximately 1.30 m below surface

Collected by metal tools rinsed with MeOH and DCM

⇒ wrapped in aluminum foil

⇒ stored at -20°C

Sample extraction

Clean up Extraction

Antonio V Herrera-Herreraa, Naima Mohamed-Rodr gueza, B rbara Socas-Rodr guezb, Carolina Mallol Development of a QuEChERS-based method combined with gas chromatography-mass spectrometry for the analysis of alkanes in sediments