Development of a quechers based methodcombined with gas chromatographymass spectrometry for the analysis ofalkanes in sediments

SAMPLE PREPARATION TECHNIQUES INANALYTICAL CHEMISTRY Lecturer: PhD.. Tran Thi Thanh Thuy GROUP 6 Development of a QuEChERS-based method combined with gas chromatography mass spectrometry

SAMPLE PREPARATION TECHNIQUES IN

ANALYTICAL CHEMISTRY

Lecturer: PhD Tran Thi Thanh Thuy

GROUP 6

Development of a QuEChERS-based method

combined with gas chromatography

mass spectrometry for the analysis of

alkanes in sediments

MEMBER ASSIGNMENTS

Pham Thi Thu Thao 20002021 Prepare Introduction, edit ppt

Le Tan Bao 20001931 Prepare Scope of application

Ho Ngoc Kieu 20005051 Prepare Meaning of method, present

Tran Thi Hong Phuc 20005541 Prepare Principle of method

Ho Hong Phuc 20001841 Prepare QuEChERS Extraction, present

Tran Anh Tu 20035401 Summarize contents, edit ppt

APPLICATION

2 3 4 5

INTRODUCTION

MEANING OF

METHOD

PRINCIPLE OF METHOD

QuEChERS

EXTRACTION

1

INTRODUCTION

Quick

Easy

Cheap

Effective

Rugged

Safe

• Was introduced in 2002

•  A type of dispersive solid

phase extraction (dSPE) used for sample preparation

• Determine 29n – alkanes (C12 – C40) and two isoprenoid acyclic hydrocarbons (pristane and phytane) in sediments

✓

Simple Effective

High quality results

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APPLICATION 2

Extract alkanes from C22  to C34 

with an odd to even predominance,

highest in C31 This is consistent

with a vascular land plant input.

These results can be used for

further paleoclimate investigations.

Lady-fern from Vascular plants

MEANING OF METHOD

3

⏳

✓

✓

✓

✓

✓

Low cost Short analytical time Reduction of solvent consumption and

possible associated contamination

Avoid the need for sophisticated devices

Diminish possible auto-oxidation reactions

Alkane extraction by QuEChERS method would be more

in line with the principles of Green Analytical Chemistry

Develop a method:

PRINCIPLE OF METHOD

4

Partitioning dSPE clean up

LC-MS

• The analyte in the sample is extracted in a suitable solvent

• The analyte in the extract is cleaned with an impurity adsorption

EXTRACTION 5

Samples were collected from archaeological sites in  Austria and Spain

Samples is described as homogeneous beige silt, approximately 1.30 m below surface

Collected by metal tools

  rinsed with MeOH and DCM

  ⇒ wrapped in aluminum foil

  ⇒ stored at -20°C

SAMPLE SELECTION

Sample extraction

Clean up Extraction

 Antonio V Herrera-Herreraa, Naima Mohamed-Rodrgueza, Brbara Socas-Rodrguezb, Carolina Mallol

Development of a QuEChERS-based method combined with gas chromatography-mass spectrometry for the analysis of alkanes in sediments