BO NONG NGHIEP VA PHAT TRIEN VIEN KHOA HQC VA CONG NGHE NONG THON VIET NAIM CHUONG TRINH UD CNSH TRONG NONG NGHIEP BAO CAO T O N G H O P KET QUA KHOA HQC CONG NGHE DU AN Hoan thien cong nghe siin xuat che pham vi sinh vat doi khang phdng benh thoi re cho cay ca phe, bong vai viing Mien Trung, Tay Nguyen Co' quan chii tri Du an: Lien hiep KHSX Cong nghe Sinh hoc va Mdi trudng, Vien Cdng nghe sinh hoc, Vien Khoa hpc va Cdng nghe Viet Nam Chii nhiem Du an: TS Pham Viet Cudng Dien thoai: 04.37568261 Fax: 04.37568261 Co" quan chii quan : Vien Cdng nghe Sinh hoc Ha Noi -12/2009 Lien hiep KHSX CNSH & MT Dtr ail san xuat t h u nghiem BO NONG NGHIEP VA PHAT TRIEN VIEN KHOA HOC VA CONG NGHE V l f T NAM NONG THON CHUONG TRINH UD CNSH TRONG NONG NGHIEP BAO CAO TONG HOP KET QUA KHOA HQC CONG NGHE DU AN Hoan thien cong nghe san xuat che pham vi sinh vat doi khang phong benh thoi re cho cay ca phe, bong vai viing Mien Trung, Tay Nguyen Co quan chd tri Du an ddu) Chii nhiem Du an (ky ten) PGS.TS Pham Viet Cudng Ha Noi - 12/2009 ~TRi^GPH6NQ0LTH MAI 1H( THANH Lien hiep KHSX CNSH & MT Du an san xuat thu nghiem DANH SACH CAC CAN BO THAM GIA THUC HIEN DU AN Ho va ten Do'n vj cong tac Nguyen Thi Kim Ctic Vien Cdng nghe Sinh hpc Pham Viet Cudng Lien hiep KHSX Cdng nghe Sinh hoc & Moi trudng Pham Due Thuan Lien hiep KHSX Cdng nghe Sinh hpc & Mdi trudng Truang Ba Hiing Lien hiep KHSX Cdng nghe Sinh hpc & Mdi trudng Le Thi Hdng Minh Lien hiep KHSX Cdng nghe Sinh hpc & Moi trudng Le Quang Quyen Vien NC cay bdng va phat trien ndng nghiep Nha Hd Le Hieu Phung Cdng ty Phan bdn Hoa sinh Sdng Kon Binh Dinh Nguyen Thanh San Xi nghiep phan bdn POLYFA cdng ty ca phe Buon Ma Thudt Le Quang Dinh Cdng ty Lam nghiep Gia NghTa Lien hiep KHSX CNSH & MT Du an san xuat thu nghiem DANH MUC CAC KY HIEU, CAC CHU VIET TAT UD CNSH U'ng dung Cdng nghe Sinh hpc KHSX Khoa hpc san xuat MT Mdi trudng NC Nghien cuu HCVSDK Htru ca vi sinh Ddi khang BMT Budn Ma Thuat MTSX Mdi trudng san xuat CP Co phan Lien hiep KHSX CNSH & MT Dir an san xudt thir nghiem DANH MUC CAC BANG Bang I: Bd tri thi nghiem thu dpc tinh cap mSu Bang 2: Anh hudng ciia phan mdi trudng len sinh khoi vi khuan Bang 3: Anh hirdng ciia nhiet dp len mat dp ciia TH 10 va DC29 Bang 4: Mat dp cua cac chiing vi khuan nghien ciru dudi cac pH mdi trudng ban dau khac Bang 5: Mat dp cua cac chung vi khuan tuyen chpn theo luu luang Bang 6: Tdng hap cac thdng sd cdng nghe cho qua trinh len men chim thu hdi sinh khdi chiing vi khuan sir dung de san xuat che pham Bang 7: Anh hudng ctia nhiet dp len sinh khdi ciia Chromocleista cirmaba CC510 Bang 8: Mat dp cua chung vi nam Chlomocleista cinnaba CCS 10 vdi cac tri sd pH ban dau khac cda qua trinh len men Bang 9: Mat dp cua chiing vi nam Chlomocleista cinnaba CC510 theo luu lugng Bang 10: Cdng thiic mdi trudng thir nghiem Bang 11: Mat dp vi sinh vat sau cac khoang thdi gian len men Bang 12: Tdng hop cac thdng sd cdng nghe tdi uu cho qua trinh len men ciia chung Chromocleista cinnaba CCS 10 sir dung cho qua trinh san xuat che pham Bang 13: KSt qua sir dung mdi trudng qua trinh len men chung xa khuan Bang 14: Cac diSu kien cdng nghe tdi uu cho qua trinh len men cua chung xa khuan Steptomyces nigrisceus VCM 1172 Bang 15: Anh hudng cua phSn co chat len sinh khdi ciia chiing VSV Bang 16: Anh hudng cila nhiet dp len mat dp cua vi sinh vat Lien hiep KHSX CNSH & MT Du an san xudt t h i nghiem Bang 17: Mat dp cua cac chimg vi sinh vat theo luu lupng Bang 18: Anh hudng ciia dp am len mat dp vi sinh vat Bang 19: Mat dp cua vi khuan mdi trudng sau ly tam Bang 20: Mat vi sinh vat tdng sd va hoat tinh ddi khang ciia che pham Bang 21: Danh gia phan ca chat len mat dp cac chung vi sinh vat Bang 22: Anh hudng ciia nhiet dp len mat dp vi sinh vat Bang 23: Danh gia mat dp vi sinh vat cua che pham ddi khang (CFU/g) theo thdi gian Bang 24: Ket qua theo doi trpng lupng ciia chudt sau 10 r^ay udng dich nudi DC29vaTH10 Bang 2S: Kit qua theo doi trpng lupng ciia chupt sau 10 udng dich nudi Bang 26: Mat dp vi sinh vat tdng sd theo loai ca chat va thdi gian ii Bang 27: Dpng hpc ciia nhiet dp qua trinh len men co chat hiiu ca Bang 28: Thanh phin phan bdn hiiu ca vi sinh ddi khang Bang 29: Danh gia mat dp vi sinh vat cua che pham ddi khang Bang 30: Tinh hinh benh Id cd re Bang 31: Anh hudng ciia che pham len su phat trien cua bp re va chieu cao cua cay sau 20 mam Bang 32: Danh gia anh hudng cua che pham len cac yeu td cau nang suat Bang 33: Tinh hinh benh hai va thdi gian phat due ciia cay Bang 34: Cac ySu td ciu nang suat va nang suat ly thuyet Bang 35: Hieu qua kinh te Bang 36: Nang suit tai cac dia diem khao nghiem Bang 37: K6t qua tap hop thu nghiem dien rdng cua ba vimg khao nghiem Bang 38: Tinh hinh benh hai cua cac cdng thiic Bang 39: Nang suat ca phe hat Lien hiep KHSX CNSH & M T Du an san xudt thu- nyhiem Bang 40: Luang toan hieu qua kinh te d cac cdng thdc sii' dting phan huu ca vi sinh Bang 41: Nang suat ca phe nhan lai Dak Genh Bang 42: Nang suat ca phe nhan tai Krdng Bdng Bang 43: Nang suat ca phe nhan tai Budn Ddn Bang 44: Bang tdng hgp kit qua su dung phan bon HCVSDK tren ddi lugng cii phe vdi cdng tuc bon CT3 Bang 45: Cdng thuc bdn phan cho cay bdng ludi Bang 46: Cdng tliiic bdn phan cho cay ca phe vdi kinh doanh nam thu Bang 47: Kk qua md hinh sir dung phan bdn ddi khang ddi vdi cay Bdng vai Bang 48: Kit qua md hinh sir dung phan bdn ddi khang ddi vdi cay ca phe Lien hiep KHSX CNSH & MT Du an san xudt thii- nghiem MUC LUC Chuong I: Tong quan 11 1.1 Mgt sd benh cay thudng gap vi sinh vat 12 1.2 Xuit xii ciia Du an 14 1.3 Sukhac giua cdng nghe da nghien cuu va Duan 17 Chuong II: Noi dung cac kit qua thuc hien da dat dugc 19 2.1 Nguyen lieu va phuang phap nghien cuu 19 2.1.1 Nguyen lieu 19 2.1.2 Phuong phap 20 2.1.3 Dia dilm va ca sd vat chat de thuc hien Duan 22 2.2 Kit qua dat dugc 23 A Hoin thien cong nghe 2i 2.2.1 Hoan thien cdng nghe len men chim 23 2.2.1.1 Lira chgn mdi trudng nudi cay thich hgp cho len men chim chung vi khuan ddi khang 23 2.2.1.2 Dilu kien len men toi uu cho chiing vi khuan Serratia marcenscen TH 10 va P chlororaphis DC29 24 2.2.1.3 Hoan thien cdng nghe len men chim, thu nhan sinh khdi chung vi nam ddi khang 27 2.2.1.4 Hoan thien cdng nghe len men chim, thu nhan sinh khdi chung xa khuan ddi khang 32 2.2.2 Thilt lap cdng nghe len men xop thu nhan sinh khdi dan chiing cac vi sinh vat ddi khang vdi budng len men Sm 2.2.2.1 Lira chpn CO chit nudi cay phu hgp vdi cac chung 33 33 Lien hiep KHSX CNSH & MT Du an sdn xuat thu nghiem 2.2.2.2 Xac dinh nhiet dp len men 34 2.2.2.3 Xac dinh dieu kien cung cap khdng cho len men xdp 35 2.2.2.4 Xac dinh dp am cho qua trinh len men xdp hai (da) chung vi sinh vat 2.2.2.5 Xac djnh thdi gian bao hp chat lupng ciia che pham ddi Idiang 36 37 2.2.3 Thiet lap cdng nghe len men xdp thu nhan sinh khdi da chimg cac vi sinh vat ddi khang vdi budng len men m' 38 2.2.4 Danh gia dp an loan ciia cac chirng vi sinh vat len chupt thi nghiem 42 2.2.5 Hoan thien cdng nghe len men ii cac loai ban pham hiru CO va che pham vi sinh ddi khang goc 45 So dd quy trinh san xuat che pham vi sinh vat ddi khang 51 Thuyet minh quy trinh san xuat che pham vi sinh ddi khang gde 52 Sa dd quy trinh san xuat phan bdn ddi khang tren nen co chat khdng trimg Thuyet minh quy trinh san xuat phan bdn HCVS ddi khang 55 56 B Banh gid hieu lure cda sdn phdm len idi tuang cdy 57 2.2.6 Danh gia hieu luc ciia san pham len ddi tuong cay bdng vai 57 2.2.6.1 Danh gia hieu lire ciia phan bdn ddi khang POLYFA len cay bdng vai tren dien hep quy md 3.000 m" 57 2.2.6.2 Danh gia khao nghiem hieu luc cila Phan bdn Vi sinh ddi khang vai quy md lOha 2.2.7 Danh gia hieu luc ciia san pham len ddi tugng cay ca phe 60 64 Lien hiep KHSX CNSH & MT Du an sail xuat thu nghiem ddng/ha Nhu vay, viec su dung phan htru co vi sinh la rit cin thilt, vtra dem lai hieu qua kinh te cao vtra bao ve mdi trudng Khiio nghiem tren dien rgng 10 Nang suat ca phe nhan d ciic Id bdn phan HCVS cao han 20% so vdi Id ddi chung d dia diem khao nghiein la Dak Ginh va Krdng Bdng, cdn d Buon Ddn la 17% Ngoai ra, che pham phan htru co vi sinh cdn cd hieu qua phdng trir nam benh cho cay ca phe Qua cac ket qua khao nghiem tren dien hep va tren dien rdng d dia ban khac dja bim tinh Dak Lak, Dac Ndng: Dak Genh, Krdng Bdng, Buon Ddn da chirng td dugc hieu qua ciia phan htru ca vi sinh ddi vdi nang suat cay bdng vai va cay ca phe Nhin chung niing suat ctia cac Id cay bdng vai va cay ca phe thi nghiem ke ca bdn phan don, phan chudng hay phan hiru co deu dat nang suat tren mirc trung binh loan qudc Quy trinh su dung phan bdn HCVS dii khang cho cay ea phe kinh doanh nhu sau: Bdn lOOOkg phan HCVSDK, giam 20% phan khoang): (160 ure + 320 Lan + 80kg Kali + lOOOkg HCVS ddi khang)/ha Bdng 46: Cdng thirc bdn phdn cho cdy cd phe vdi kinh doanh ndm thii- Dot bdn Loai phan Phan HCVS ddi khang Dgt Bdn vao diu miia mua 500 Dpt Dgt Bdn vao dgt tudi thu 500 Dgt Trudc thu hoach thang Lien hiep KHSX CNSH & t TJ Du' an san xujt thii' nghiem 80 50 160 160 25 P2O5 '1 30 30 C Td chiic san xuit Lien hiep Khoa hge san xuit Cdng nghe sinh hpc va mdi trudng da td chuc, hoan thien pilot sin xuit dugc 5100 kg chl phim vi sinh vat ddi khang glc va mdt phin phan bdn ddi khang tren nin huu co khdng trting Dao tao can bd cdng nhan, va chuyin giao cdng nghe san xuit cho Xi nghiep phan bdn POLYFA thudc Cdng ty ca phe Budn Me Thudt, DSc Lie, Cdng ty TNHH hda sinh huu ca Polyfa, Cdng ty Lam nghiep Gia NghTa, Die Ndng, Cdng ty cd phin phan bdn sinh hda Sdng Kon, Binh Djnh Tham gia cting cac cdng ty da san xuit dugc tren 5100 tin phan bdn HVSDK D iTng dung mo hinh bdn phan HCVSDK Polyfa a Cay bdng vai ( gidng bdng ludi VN-OI-02) Ddi vdi cay bdng cd 25 hd d xa Tan hda huyen Budn Ddn Die lie su dung gan 50 cac hd dugc hudng dan bdn theo dung quy trinh da neu tren cho eay bdng nhu d bang 42 Bdng 47 Ket qud md hinh sit dung phdn bdn ddi khdng dot vdi cdy Bdng vdi Tinh hinh benh re khao sat Nang suat % tang so trung binh vdi ddi (tan/ha) Sdhd Ndi dung - chirng Khdng bdn 1,7% 1,35 - Bdn phan 13 Khdng xuat hien 1,78 31 P 0.0064 Lien hiep KHSX CNSH & MT Du an san xuat thu nghiem Ket qua cho thay khdng cd benh thdi cd re, nang suit cua md hinh dat 17,8 ta /ha trng klii nang suat binh quan dai tra khdng bdn la 13,5 ta/ha b Cay ca phe ( lii loai ca phe vdi kinh doanh Robusta)- Dia dilm xay dung md hinh tai xii Bang A.Dranh, huyen Krong Ana Dac LJe Hgi ndng dan dam nghiem cd 13 hg gia dinh tham gia vdi dien tich 50 va lay ddi chtrng la hd vting khdng bdn phiin HCVS ddi khang Bdn phan theo dung quy trinh nhu md ta d bang 46 Bang 48 Ket qua md hinh sii- dung phdn bdn ddi khdng dot vdi cdy cd phe Sdhd khao sat % tang so trung binh Tinh hinh benh re Nang suat P vdi ddi (tan/ha) Ndi dung chtrng Khdng bdn 2%) 5,7 Bdn phan 13 Klidng xuat hien 6,55 15 0.0087 Kit qua cho thiy vdi md hinh quy md khoang 50 cho thiy rang cay ca phe eho nang suit cao ban va khdng thiy xuat hien benh thdi re E Dao tao: Du an tham gia dao tao nghien cim sinh, va mdt sd can bd ky thuat qua trinh hoan thien cdng nghe, 02 ky thuat vien KCS va mgt sd cdng nhan ky thuat qua trinh san xuat 76 Lien hiep KHSX CNSH & MT Du' an ,san xu^t thtr nghiem DANH GIA SO SANH CONG VIEC DA THUC HIEN V(Jl NQI DLING DANG KY TT Ngi dung theo thuyit minh Ngi dung da thuc hien Ghi chii Hodn thien cdng nghe len Da thuc hien cac thi nghiem hoan men chim thu nhdn sinh khdi thien va dn dinh eac thdng sd Hoan cdc chimg vi khudn ddi khdng, cdng nghe: pH, nhiet dp, lugng thu nhdn sinh khdi cdc chting khi, vdng quay, phin mdi vi ndm ddi khdng thu nhdn trudng ctia len men chim gidng sinh khdi chung xg khudn dot chiing khdng vi (Steptomyces marcescens, nigrisceus).vdi he thdng len chiing vi khuan Serratia P chlororaphis nam Chromocleista men 100 lit vd tgo che phdm cinnaba CC510 va chting xa ddi khdng goc khuan Steptomyces nigrisceus VCM 1172 Thiet ldp cdng nghe len men -Da thuc hien cac thi nghiem hoan Hoan xdp thu nhdn sinh khdi dan thien va chung dn djnh cac thdng sd cdc vi sinh vdt ddi cdng nghe: nhiet do, lugng khdng vdi budng len men m thdi, dp am, phan mdi trudng ciia len men xdp nhan gidng chung vi khuan Serratia marcescens, P chlororaphis rieng biet budng len men m' -Da danh gia kha nang thu hdi sinh khoi cua hai chiing vi nam va xa khuin bing each trdn them chat Du an san xuJt thu' nghiem Lien hiep KHSX C N S H & M T hap phu vao mdi trudng nudi cay.linn giau biing ly tam, phdi trgn cimg chiing vi khuan - Danh gia dugc kha nang bao hg che pham theo thdi gian, san pham den 12 thang van cdn dam bao chat luong Thiet lap cdng nghe len men -Da xay dung cac thi nghiem de Da hoan xdp thu nhdn sinli khdi da hoan thien va on dinh cac thdng thimh chung cdc vi sinh vdt ddi sd cdng nghe: nhiet dp, lugng khdng vdi budng len tnen in thdi, dp im, phan mdi trudng cua len men xdp nhiin gidng cting mdt liic chting vi khuin Serratia marcescens, P chlororaphis budng len men m' - Danh gia dugc kha nang bao hp chl phim theo thdi gian, san pham din 12 thang vin cdn dam bao chit lugng tuong duang vdi len men don chiing Hodn thien cdng nghe len men il cdc logi bdn thdnh phdm hiru ca vd chi phdm vi sinh ddi khdng gde vdi thi tich khdi u 90 - 100m' di sdn xudt che - Da xae dinh dugc phan Da hoan ban phim hiru co ttr vd ca phe, than bun va bim mia - Da xac dinh dugc thdi gian chin cua khdi u sau len men phan Lien hiep KHSX CNSH & MT Dir an san xuat thir nghie; ghiem phdm VSDK tren nen ca chdt htiy hiru co phti hgp vdi nhiet dp khdng tritng phat trien cua cac chiing vi sinh phdi trdn - Dii xac djnh ky thuat dn djnh am cho khdi u - Cdng thtrc phdi trgn cac trung lugng - Danh gia thdi gian bao quan ctia san pham phan bdn Ddnh gid hieu lire ctia sdn -Da phdi hgp cting Vien nghien Da hoan phdm len ddi tirang cdy bdng ctru Bdng va phat trien ndng vai nghiep Nha hd Ninh Thuan danh gia khao nghiem: - Tren quy md dien hep 3000m^ tai CuM'Nga- Daklak - Tren quy md dien rdng lOha tai dja diem thudc tinh Dak Lak: Dak • Ginh, Krdng Bdng, Budn Ddn cho viing sinh thai tai Dae lae - Da dua dugc quy trinh sir dung phan bdn cho cay ca phe -Ca hai quy md de cho thay san phim cd tac dung lam giam ty le benh Id cd rl cho cay bdng va cd hieu qua kinh te ! Lien hiep KHSX CNSH & MT Du an san xuat thii' nghiem Ddnh gid hieu lire cita sdn -Da phdi hgp cting Cdng ty ca phe Da hoan phdm len ddi tirang cdy cd phe Budn Ma Thudt va mgt sd dan vi khac xay dung md hinh bdn phan cho cay ca phe va danh gia khao nghiem tren dien rdng va dien hep tai DakLak, Dac Ndng la: Dak Genh, Krdng Bdng vii Budn Ddn - Da danh gia tinh hinh benh Id cd re , nang suat vii hieu qua kinh te sir dung phan bdn vi sinh ddi khang ! - Da dua dugc quy trinh sit dung phan bdn cho ciiy ca phe San pham da hoan Ghi chti Quy trinh san xuat che pham 01 quy trinh san xuat che pham vi Da hoan vi sinh vat ddi khang gde sinh vat ddi khang gde phdng phdng chdng benh thdi cd re chdng benh thdi cd re cay ca phe cay ca phe va cay bdng TT va cay bdng Siin pham theo thuyet minh ciia duan Quy trinh san xuat che pham 01 quy trinh san xuat che pham VSDK tren nin co chat khdng VSDK tren nin co chit khdng trting Da hoan thanh trting Quy trinh bdn phan VSV dot Quy trinh bdn phan VSV dot Da hoan Dir an san xuat thir nghiem Lien hiep KHSX CNSH & MT khang cho cay bdng vai, cay khang cho cay bdng vai, cay ca ca phe phe Mo hinh str dung phiin bdn Tren IOO ha, nang suat tang so vdi ddi chtrng 15% ddi vdi ca phe va 31 % ddi vdi cay bdng vai Che pham VSV ddi khang gde San xuat dupe khoang tren 5000kg 5000kg - Mat dp vi sinh DK: lO'' CFU/g - Mat dp vi sinh DK: lO'" CFU Che pham VS DK tren nen ca San xuat dugc khoang 5100 tan chit khdng trting 5000 tan Mat vi sinh D K - I O " CFU/g - Mat dp vi sinh DK: lO'CFU -HO'U C O - - % - Htru ca: 15,6 % -N-1,2% - N : l%i - P: 3%) - K : 1% -P-2,9-3,1 % - K-1- 1,05 % -Vi lugng khoang 0,001 - V i lugng: 0,001% Dao tao nghien ciiu sinh Mdt sd can bg cdng nghe va cdng nhan cho cac co sd san xuat Cdng trinh cdng bd bai bao trinh tu gen cdng bd ngan hang gene Lien hiep KHSX CNSH & MT Dir an san xuSt thir nghiem C H U O N G III: K E T LUAN Dii hoan thien cdng nghe san xuiil che phiim vi sinh vat dii khang gde qua hai cdng doan vdi ciic thdng sd ky thuat sau: Ddi vdi cdng doan marcescensTHlO len men chim, chiing vi khuan Serratia vd Pseudomonas chlororaphis DC29 dugc dugc nuoi cay mdi trudng MTl cd cac thdng sd:nhiet dp - 30-35 "C: pH-7,0-7,2: toe dp canh khuii\ 150 \/pli: lugng nap 601/phut; thdi gian thu hdi 36-40 cho mat dp cao nhat Iu 2.10" 5.10''CFU/ml Chting vi nam Chromocleista cinnaba CCS 10 dat duoc hieu qua nliit 4,3.10* CFU/ml len men vdi eac chi sd: nhiet 35"C; pH6,5; tdc dp canh khuiylSO v/ph,lupng nap 701/; thdi gian thu hoi 40 Chiing xa xuin Sleplomyces nigrisceus VCM 1172 dat dugc hieu qua nhat 7././0'CFU/ml len men vdi cac chi sd: nhiet dp 45°C; pH6,8-7; tdc dp canh khuiy200 v/ph,lugng nap 601/; thdi gian thu hdi 48-52h Ddi vdi len men xdp chting vi nim vii xa khuan: So sanh len men dan chting va len men chung chting, thiy ring mac dti cd khac ve nhiet dp vii thdi gian nudi eiy, nhung len men da chiing vdi cac thdng sd: dp i m - 40%; nhiet dp 40 "C, thdi gid 6501/ph, mdi trudng, cam, btin mia, vd ca phe (3:2:1) thu hdi sau 48-52 h cho hieu qua cao nhat Da hoan thien cdng nghe len men ii cac loai ban phim hiiu eo va ehl phim vi sinh ddi khang gde vdi thi tich khdi ti 90 m' - 100m' dl san xuit chl phim VSDK tren nin co chit khdng trung vdi cac dilu kien: ngudn co chit la btin mia: than btin: vd ca phe theo ti le 1:1:1; im ca chit 40 % - 50 %, pH tu 6-5 din 7, nhiet ttr 35-C - 40°C, chl pham vi sinh vat gde 1kg/ Itan (mat dp 10' CFU/g) Thdi gian len men tir 4-10 Mat dp VSVDK d,t 3.4.\0' CFU/g Lien hiep KHSX CNSH & MT Du an san xuSt thu nghiem Dii khao nghiem tren md hinh dien rdng va dien hep cho cay ca phe vii cay bdng ket qua cho thay: Ddi vdi ciiv bdni^ vai: Cae cdng thirc bdn phan huu co vi sinh ddi khang POLYFA c6 bd sung them phan don cho dii lugng (120 kg N + 60 kg P2O5 + 60 kg K20)/ha khong thay xuat hien benh Id cd re nam F oxporum gay hai, cdn cac Id ddi chung (khdng dugc bdn phiin) cd xuat hien benh re nhung ti le nho ttr 3,3% den 0,02% Nang suat: Tiit ca cac viing khao nghiem vdi dien rdng deu cho nang suat cao ban so vdi khdng bdn tren 10% Hieu qua kinh le: phiin thu sau trtr chi phi bdn phan htru ca vi sinh doi khang Idn han cdng thtrc ddi chtrng bdn phan don va lai tren 743.000 ddng/ha Ddi vdi ca phe: Khao nghiem dien hep cOng nhu dien rdng, nang suat ca phe nhan d cac Id bdn phan HCVS cao hon ttr 17 din 20% so vdi Id ddi chtrng hieu qua phdng trtr nim benh cho cay ca phe cao Da dua quy trinh san xuit chl phim va phan bdn dii khang va quy trinh su dung phan bdn cho cay ca phe va cay bdng Da tham gia dao tao nghien ciru sinh va mgt sl can bg ky thuat, edng nhan san xuit phuc vu Du an vii cac dan vi chuyen giao khac Lien hiep KHSX CNSH & MT Du an san xufit thii' nghiem BO NONG NGHIEP VA PHAT TRIEN VIEN KHOA HQC VA CONG NGHE NONG THON VIET NAM CHUONG TRINH UD CNSH TRONG NONG NGHIEP PHU LUC DVAN Hoan thien cong nghe san xuSt che pham vi sinh vat doi khang phong benh thoi re dio cay ca phe, bong vai viing Mien Trung, Tay Nguyen Chil nhiem Duan Co quan chu tri Du an (j^ ten) C^'™' ^°"S ''°"^ PGS.TS Pham Viet Cudng Ha Noi-12/2009 Lien hi$p KHSX CNSH & MT Hoat Dv kl siin xuit thu nghifm mimmnsFoxysporum cua cac cnung vi sinh vat 78 ffi^'wV'-^ V1$N THO NHUONG NONG HOA PHONffi KIEM NGHIEM VI SINH VAT NONG NGHIEP t^-Ddng-NggcJ/TirLiem - Hd Npi DT: 844.37522125, Fax: 844.38389924 PHIEU KET QUA KIEM NGHIEM Sd: /PKNVSVNN Noi gtri miu: Lien hiep Khoa hge san xuit Cdng nghe Sinh hge va Moi trudng 18 Hoang Qudc Viet So lugng miu: 01 Yeu eau phan tich: Vi sinh vat ddi khang nim Ngay nhan mau: Ket qua: Ten mau kiem nghiem Chi tieu kiem nghiein Tieu chuan kiem nghiem Phan bdn vi sinh ddi Vi sinh vat ddi khang khang Ket qua Idem nghiem (CFU/g) lOTCN 867-2006 3,3x10° POLYFA nam 1.3.1 (CF131) Ha Ndi, /a thang J-nam 2008 Trudng phong kiem nghiem Kiem nghiem vien Nguyin Thu Ha Trin Thj Thanh Thiy Kk qua nir, chi CO gta tn Iren miu gm phan lich Qui Ml hm 30 ngiy « l u Ira kit qua ma khong CO stf khiiu nqi mo phong him nghiim se lihong chiu Irach nhiem ve mau gm ^^ VI£N T H NHU'ONG NONG HOA PHONG KIEM OI.NH CHAT LUdNG PHAN BON Sd 11 CONTROL OF FERUUZERS QUAUTY No 11 Dja chl: Oong Ngge - Tif U6m - Ha N6i; OlSn thogl: 04.8365636 - Fax; 04.8389924 Ha Ndi ngdy jtAhdng Sndm 2003 So: 136IKDPBI200 PHIEU KET QUA THU NGHIEM Dan VI giii mSu: Liin hiSp Khoa hpc siin xuai Cpng nghe sinh hpc va Moi trudng Dia chi: 18 Hoimg Qudc Viet Q Cia Gia'y, Ha Npi Ngay gtri miu: ijl (F^/lOOtJ Ngiiy tra kei qua: / Z / / /200^ Loai miu: Philn bdn vi sinh ddi khang POLYFA 1.3.1 (CF13I) Sd lugng miu: I Yeu c i u p h i n tich: HOu ca (OM), N tdng so (Nts), P httu hi6u (Phh), K hoa tan (Kht) KSi qua thtr nghiSm: (Dim vi tick: Kei qud phdn lichl Mdu thuang phdm) OM TT Nts Phh % P,05 Kht % K,0 19.0 1.10 3.10 1.05 % Phan bon vi sinh ddi khang POLYFA L3.1(CF131) Phuang phap xac dinh: 10 TON 366 -2004, TCVN 5815- 2001 Phu trach Phong KDCL phan bdn Sd 11 frudng phdng Phan tich Trung tam TS Nguyftn Van Chien Phan lich vien: NI Lan, L.K Dung nao, PhOm phan tich se khang chia trach nhiim vi mia gui ... chiing vi sinh vat ddi khang Hon nua, vdi muc nhiet dp kliac tao dieu kien cho cac loai vi sinh vat khac canh tranh phat trien vdi cac chirng vi sinh vat su dung Vi vay viec khdng che nhiet dp cho. .. thoi re cho cay ca phe, bong vai viing Mien Trung, Tay Nguyen Co'' quan chii tri Du an: Lien hiep KHSX Cong nghe Sinh hoc va Mdi trudng, Vien Cdng nghe sinh hoc, Vien Khoa hpc va Cdng nghe Viet Nam... KC04-04 Vien KTNNVN chil tri, De tai Khoa hge Co ban Ma sd 82- 03.04, De tai cap Bd Vien Khoa hpc & Cdng nghe Viet Nam Vien Cdng nghe sinh hpc chu tri da phan lap tuyen chpn dugc mdt sd chung vi sinh