In the absence of calcium the molecule is compact but in the presence of calcium, as its six similar domains alter their relative position, a generally more open configuration is adopted
Trang 1Co-operation of domain-binding and calcium-binding sites
in the activation of gelsolin
Emeline Lagarrigue1, Sutherland K Maciver2, Abdellatif Fattoum3, Yves Benyamin1and Claude Roustan1 1
UMR 5539 (CNRS) Laboratoire de motilite´ cellulaire (Ecole Pratique des Hautes Etudes), Universite´ de Montpellier 2, France;
2
Genes and Development Group, Department of Biomedical Sciences, University of Edinburgh, Scotland, UK;
3
Centre de Recherches de Biochimie Macromole´culaire, UPR 1086 (CNRS), Montpellier, France
Gelsolin is an abundant calcium dependent actin filament
severing and capping protein In the absence of calcium the
molecule is compact but in the presence of calcium, as its six
similar domains alter their relative position, a generally more
open configuration is adopted to reveal the three actin
binding sites It is generally held that a helical-latch at the
C-terminus of gelsolin’s domain 6 (G6), binds domain 2 (G2)
to keep gelsolin in the calcium-free compact state, and that
the crutial calcium binding site(s) reside in the C-terminal
half of gelsolin perhaps involving the C-terminal helix itself
has to be bound to release this latch Here we provide
evidence for a calcium dependent conformational change
within G2 (Kd¼ 15 lM) We also report a calcium
dependent binding site for the C-terminus (G4–6) within
G2 and delimit this further to a specific region formed by
residues 203–225 and 159–193 It is known that the acti-vation of gelsolin involves multiple calcium binding events (around 6) the first of which (in G6) may release the latch We propose that the calcium-dependent con-formational change in G2 may be a subsequent step that
is necessary for the dissociation of G2 from G4–6, and that this movement occurs in sympathy with calcium induced conformational changes within G6 by the physi-cal coupling of the two physi-calcium binding sites within G2 and G6 Additional calcium binding in other domains then result in the complete opening and activation of the gelsolin molecule
Keywords: actin; gelsolin; cytoskeleton; severing; calcium activation
Gelsolin is a calcium-activated actin filament severing and
capping protein found in many tissues and in the plasma of
vertebrates (for a review, see [1]) It belongs to a wider group
of actin-binding proteins that share a number of repeated
domains; six in the case of gelsolin itself, adseverin and
villin, and three in capG, fragmin and severin (for a review,
see [2]) The binding of calcium to gelsolin and to actin
bound gelsolin is complex Free gelsolin binds at least six
calcium ions These sites, coordinated solely by gelsolin have been termed type II [3] The affinity of type II sites varies greatly High affinity calcium sites (Kd 1 lM) have been identified [4–6] and two of these have been localized within G4–6 [7] A body of evidence suggests that calcium binding
by G4–6 affords calcium-sensitivity to the whole gelsolin molecule [8,9] Sites have been identified by biochemical means within G4-5 (Kd 2 lM) and G5–6 (Kd 0.2 lM) [9], and crystallographic studies (S Kolappan, J Gooch,
A Weeds & P McLaughlin, Wellcome Centre for Cell Biology, University of Edinburgh, UK, personal commu-nication, [3]) have shown that calcium ions are bound by both G5 and G6 (sites IIG5 and IIG6 [3] Low affinity calcium-binding sites (Kd 1 mM) have also been detected [10,11] A site has been inferred to lie within G2-3 by proteolysis susceptibility and molecular radius changes [12], and this site has been narrowed further to G2 (IIG2) and tentatively suggested to have a dissociation constant of
32 lM[11] Additionally, a calcium ion (IG1) is trapped between actin and G1 [13,14], and by actin and G4 (IG4) [3,15], these calcium sites, coordinated by both gelsolin and actin have been termed type I sites [3]
In the absence of calcium, gelsolin cannot bind actin as its three [5,16] identified actin binding sites residing in G1, G2 and G4 [7,16,17] are not accessible In the presence of calcium, gelsolin becomes activated by the unfolding of the whole molecule so that the F-actin binding region in G2 is exposed allowing the molecule to make the initial contact with the actin filament Whereas gelsolin is opened by 0.1–1 lM calcium [12,18,19], the ternary actin: gelsolin complex is only stable at calcium concentrations exceeding
Correspondence to C Roustan, UMR 5539(CNRS) UM2 CC107,
Place E Bataillon 34095 Montpellier Cedex 5, France.
Fax: + 33 0467144927,
E-mail: roustanc@crit.univ-montp2.fr
Abbreviations: G1-6, The six repeated domains of gelsolin; FITC,
fluorescein 5-isothiocyanate; 1,5-I-AEDANS,
N-iodoacetyl-N¢-(sulfo-1-naphthyl)-ethylenediamine; BACNHS, biotinamidocaproate
N-hydroxyl-succinimide ester; G-actin, monomeric actin;
F-actin, filamentous actin.
Note: we have adopted the labeling system introduced by Choe et al.
[Choe, H., Burtnick, L.D., Mejillano, M., Yin, H.L., Robinson, R.C.
& Choe, S (2002) J Mol Evol 324, 691–702.] for the various
Calcium-binding sites so that IG1 is the type I Calcium-binding site within G2 and IIG6 is
the type II binding site within G6 Type I binding sites are coordinated
by gelsolin and actin whereas type II sites are coordinated solely by
gelsolin residues.
Note: webpages are available at http://www.dbs.University-montp2.fr/
umr5539/, http://www.ephe.University-montp2.fr and
http://www.bms.ed.ac.uk/research/others/smaciver/index.htm
(Received 20 December 2002, revised 10 March 2003,
accepted 26 March 2003)
Trang 230 lM [19], and the most rapid severing rate of actin
filaments occurs at 300 lM [20] Accumulating evidence
[3,12,18,21] suggests the following mechanism for the
activation of gelsolin by calcium Low calcium
concentra-tions are proposed [18,21,22] to unlatch the connection
between G2 and G6, but higher concentrations are required
to break salt bridges between other domains until the
gelsolin is fully open, then additional calcium ions are
required to bind actin to G1 and to G4 for severing and
capping The details of the latch helix structure in the
presence of calcium are not clear as there is no density for
this region in the available crystallographic solutions [3,15],
so we cannot tell if calcium ions are bound directly to the
latch helix to inhibit its binding to G2 However, this seems
unlikely as is has been shown that gelsolin without the
C-terminal helix alters neither the number nor the affinity
of gelsolin’s calcium binding sites [18]
Here, we confirm that G2 shows calcium-dependent
conformational changes and that peptides derived from it
show calcium-dependent binding to G4–6 Therefore the
calcium-dependence shown by G2 may have a role in the
multistep mechanism of calcium-activation of gelsolin prior
to binding actin
Methods
Proteins and peptides
Human gelsolin domain 2 (G2) and domains 4–6 (G4–6)
were produced in Escherichia coli BL21(pLysS) and
BL21(de3), respectively, using the pMW172 vector [23],
following induction of expression with isopropyl thio b-D
-galactoside G2 (residues 151–266 of human serum gelsolin)
was purified from the soluble fraction of the bacteria [24],
and G4–6 (residues 407–755) [25] was purified from
inclusion bodies [26]
Gelsolin G4–6 domain was selectively cleaved by trypsin
The domain (225 lgÆmL)1) was incubated in 0.1MTris HCl
pH 7.5 supplemented with either 1 mM CaCl2 or 1 mM
EGTA in the presence of trypsin (9 lg/mL) The proteolysis
was stopped by addition of an antiprotease mixture
(Complete) (1 : 25 mass/vol.) purchased from Roche SA
(Mannheim, Germany) To identify the cleavage products,
the digest was labeled at cysteine residues by treatment with
1,5-I-AEDANS (10 molar excess) [27] for 4 h at room
temperature, in the presence of 1% SDS The labeling
reaction was stopped by addition of 0.1 M
b-mercapto-ethanol The digest was then analyzed by SDS/PAGE
Antibodies directed towards gelsolin G4–6 domain were
elicited in rabbits according to [28] Anti-IgG antibodies
labeled with alkaline phosphatase were purchased from
Sigma (Dorset, UK)
Synthetic peptides derived from gelsolin sequences 159–
193 and 203–225 [17] were prepared on solid phase support
using a 9050 Milligen PepSynthesizer (Millipore) according
to the Fmoc/tBu system The crude peptides were
de-protected and thoroughly purified by preparative
reverse-phase HPLC The purified peptides were shown to
be homogenous by analytical HPLC Electrospray mass
spectra, carried out in the positive ion mode using a Trio
2000 VG Biotech Mass spectrometer (Altrincham), were in
line with the expected structures
Gelsolin G2 and G4–6 domains were labeled by FITC as described elsewhere [29] Biotinylation of G2 domain by BACNHS was performed as reported previously [30] Excess reagent was eliminated by chromatography on a PD10 column (Pharmacia) in 0.1MNaHCO3buffer pH 8.6
Immunological techniques The ELISA technique [31], described previously in detail [32] was used to monitor interaction of ligands with synthetic peptides or G4–6 domain G4–6 domain (1 lgÆmL)1) or synthetic peptides (1 lgÆmL)1) in 50 mM NaHCO3/Na2CO3, pH 9.5, were immobilized on plastic microtiter wells The plate was then saturated with 0.5% gelatin/3% gelatin hydrolysate in 140 mM NaCl, 0.05% tween 20, 10 mM Phosphate buffer, pH 7.4 Experiments with coated fragments were performed in 0.1MKCl, 20 mM Tris pH 7.2 Binding was monitored at 405 nm using alkaline phosphatase-labeled anti-IgG antibodies (dilution 1/1000) or alkaline phosphatase labeled streptavidin (dilu-tion 1/1000) Control assays were carried out in wells saturated with gelatin and gelatin hydrolysate used alone Each assay was conducted in triplicate and the mean value plotted after subtraction of nonspecific absorption The binding parameters (apparent dissociation constant Kdand the maximal binding Amax) were determined by nonlinear fitting A¼ Amax· [L]/(Kd+ [L]) (relation 1) where A is the absorbance at 405 nm and [L] the ligand concentration,
by using theCURVE FITsoftware developed by Kevin Raner Software, Mt Waverley, Victoria, Australia Additional details on the different experimental conditions are given in the figure legends
Fluorescence measurements Fluorescence experiments were conducted with a LS 50 Perkin–Elmer luminescence spectrometer Spectra for FITC labeled proteins were obtained in 0.1M KCl 20 mMTris/ HCl buffer pH 7.4, with the excitation wavelength set at
470 nm Fluorescence changes were deduced from the area
of the emission spectra of fluorescein isothiocyanate (FITC) between 505 and 525 nm The parameters Kd (apparent dissociation constant) and Amax (maximum effect) were calculated by nonlinear fitting of the experimental data points as for ELISA (relation 1), or by using the following equation (relation 2), DF¼ 1/2 Amax· [E])1{([E] + [L] + Kd)) (([E] + [L] + Kd)2) 4[E] · [L])0.5} where [E] is the concentration of the fluorescent protein The maximum fluorescence change (Amax) at infinite substrate concentration expressed as percentage variation from initial fluorescence: F1 – F/F*100 was calculated by the relation F1 – F/F ¼ Amax/F where F and F1 are fluorescence intensities for zero and infinite ligand concen-trations, respectively
Analytical methods Protein concentrations were determined by UV absorbency using a Varian MS 100 spectrophotometer Gelsolin domain concentrations were determined spectrophotomet-rically using values of A280(1 cm)1)¼ 15.5 lMfor G4–6, and 79 l for G2 These extinction coefficients were
Trang 3calculated by tryptophan, tyrosine, and cysteine content
[33] Electrophoresis was carried out on 15% (w/v)
polyacrylamide slab gels (SDS/PAGE 15%) according to
Laemmli [34] and stained with Coomassie blue or observed
by UV for fluorescent gels before staining The 14–97 kDa
molecular weight marker kits were from Biorad Calcium
concentrations in the calcium-EGTA buffer system were
experimentally measured by fluorometry using indo 1 as
indicator [35]
Results
Cleavage of G4–6 domain by trypsin
A recent detailed study [19] reported that the exposure of
various tryptic cleavage sites in gelsolin is actin, and
calcium, dependent In fact, several investigations using
namely circular dichroism and light scattering [9,12,15,18]
showed the occurrence of major conformational changes
in the regulatory C-terminal half of gelsolin upon calcium
binding In an initial experiment, we tested the susceptibility
of the C-terminal domain to proteolysis As shown in
Fig 1A, tryptic digestion give rise to two fragments, one of
30 kDa and the other of 16 kDa In order to identify these
fragments, we have labeled the unique cysteine residue
(Cys645) located in domain G6 with 1,5-I-AEDANS As
this residue is buried in the native molecule [15,22], the
labeling was carried out after proteolytic digestion and SDS
unfolding (see Methods) Figure 1A shows that only the
16 kDa band is fluorescent This result demonstrates that
the cleavage occurs in the loop between G5 and G6 domain
and is in accord with the unpublished results reported
previously [19]
As depicted in Fig 1B, the tryptic cleavage was faster in EGTA than in calcium, suggesting that the orientation between the two domains is different and the junction more accessible in EGTA
Calcium induced change in the G4–6 and G2 domains Conformation changes induced by calcium binding were monitored by two approaches First, intrinsic tryptophan fluorescence of G4–6 domain was measured in the presence
of increasing calcium concentrations (between 1 nM and
1 mM) An increase in fluorescence intensity was observed at submicromolar calcium concentrations (Fig 2) In a second experiment, conformational changes were detected from the extrinsic fluorescence measurements of FITC-labeled G4–6 domain A biphasic relationship was observed (Fig 2) producing two transitions in fluorescence intensity, one at
1.5 lM, and another around 0.1 lM These transitions reflecting conformational changes correlate well with the two binding sites (IIG5 and IIG6, Kd¼ 2 and 0.2 lM, respect-ively) detected and measured by equilibrium dialysis [7] Intrinsic fluorescence of G2 domain was also measured as a function of calcium concentration (Fig 2 insert) A simple reduction of fluorescence was observed with half maximum change occurring at a calcium concentration of 15 lM
Interaction of G2 segment with G4–6 domain
In the crystallographic model of gelsolin in the EGTA form [22], G2 is tightly interacting with G6 segment (Fig 3) Two regions in G2 domain appear included in this interface The
Fig 2 Effect of calcium on the G4–6 tryptophan and FITClabeled G4–6 fluorescence emission Aliquots of calcium (0.1 m M solution) were added successively to unlabeled G4–6 or labeled G4–6 in 0.1 M
KCl, 20 m M Tris buffer pH 7.4 in the presence of 0.1 m M EGTA Changes in fluorescence intensities corresponding to tryptophan emission (s) or FITC emission (d) are plotted vs free calcium con-centration expressed as pCa ¼ log(1/[Ca 2+
]) Calcium concentrations were determined experimentally (see Material and methods) Inset: effect of calcium on the G2 tryptophan fluorescence emission Fluo-rescence changes were plotted vs free calcium concentrations.
Fig 1 Effect of calcium on susceptibility of gelsolin G4–6 domain to
tryptic digestion (A) Identification of the two fragments (30 kDa and
16 kDa) produced by proteolysis in the presence of 1 m M EGTA
followed by 1,5-I-AEDANS labeling as described in Material and
methods Molecular weight markers (lane T) G4–6 digest chemically
modified by IEADANS and revealed with Coomassie blue (lane 1) or
upon UV lamp (lane 2) (B) Digestion of G4–6 domain by trypsin
(trypsin/G4–6 ratio: 1/25 w/w) for 10 min molecular weight markers
(lane T) G4–6 domain (46 kDa) before proteolysis (lane 1) G4–6
digest in the presence of 0.1 m M calcium (lane 2) and in the presence of
0.1 m M EGTA (lane 3) Molecular weight marker are
phosphory-lase B (97.4 kDa), bovine serum albumin (66.2 kDa), ovalbumin
(45 kDa), carbonic anhydrase (31 kDa), soybean trypsin inhibitor
(21.5 kDa) and lysozyme (14.4 kDa).
Trang 4sequence 203–225, including one of the actin-binding sites, is
in interaction with the C-terminal a-helix of G6 domain
The sequence 159–193, including the second actin interface,
appears also in interaction with G6 domain Therefore, we
tested the interaction of G4–6 domain with G2 domain by
two independent methods
In ELISA experiments, G4–6 fragment was immobilized
on the plastic microtiter plate and the binding of
biotinyl-ated G2 domain was revealed by using alkaline phosphatase
labeled streptavidin Figure 4 shows that binding occurs in
the presence of EGTA as well in the presence of calcium
although a better affinity is observed in the former case
These data were confirmed by studies in solution using
fluorescence measurements G2 or G4–6 domains were
labeled by FITC and increasing concentrations of G4–6 or
G2 were added, respectively Figure 5A shows a decrease in
the fluorescence intensity of FITC labeled G2 domain in the
presence of EGTA Analysis of these data shows that the
fluorescence intensity decrease, extrapolated to infinite
concentration of G4–6, is low (4%) Therefore, only a
rough estimation of the apparent Kd(about 0.5 lM) can be
obtained This result shows that G4–6 fragment interaction
induces a conformational change in G2 domain in the
presence of EGTA Calcium was without effect (Fig 5A),
but it is known that calcium did promote important changes
[11,36], which would explain the present results
Conse-quently, further analyses, using FITC labeled G4–6, were
carried out Labeled G4–6 was incubated with increasing
concentrations of G2 (between 0 and 7.6 l ) and the
changes in fluorescence were monitored Saturation curves were observed in the presence of EGTA or calcium and apparent Kds of 0.8 and 3.5 lM, respectively, were deter-mined (Fig 5B and Table 1) These values and those obtained above from ELISA show a better interaction of G2 in the presence of EGTA than calcium The differences
in the absolute values observed between the two methods are likely to be due to the heterogeneous phases used in ELISA More interestingly, a significant difference in the maximum fluorescence enhancement determined in EGTA and calcium (about 8% and 30%, respectively) possibly reflects changes in domain conformation and in interfaces produced under these two conditions In order to deduce a more precise relationship between G2 interaction and G4–6 calcium-induced conformation, the maximum fluorescence enhancement extrapolated for infinite G2 concentrations was determined at various calcium concentrations As shown in Fig 6, a change in the fluorescence from EGTA to calcium states was observed in the 0.1 lM range This important result suggests that the transition would be linked
to the high affinity calcium site in G4–6 [7], that we now know to be IIG6 [3] (see Discussion) In addition, apparent
Kdvalues were also estimated from the same fluorescence experiments performed in the presence of different calcium concentrations As shown in Fig 6 insert, an apparent Kd transition occurs around 15 lM, a value which is observed for the binding of calcium to G2 domain
Footprint of G2 on gelsolin G4–6 domain Two sites within the G2 domain appear involved in the G2- G4–6 interface in the presence of EGTA (Fig 3) The interaction of these two sites (residues 159–193 and 203– 225) was first tested by ELISA The interaction of G4–6 with the plate-coated peptides was revealed using specific antibodies to G4–6 The results summarized in Table 1
Fig 3 X-ray crystallographic structure of gelsolin in the absence of
calcium (Burtnick et al [22]) showing the interface between the G6
C-terminal domain and G2 The G6 domain is coloured green and its
C-terminal alpha helix is coloured blue The G2 domain is coloured
yellow and its sequences in contact with G6, sequences 159–193 and
203–225 are coloured red and purple, respectively.
Fig 4 Binding of gelsolin domain G2 with G4–6 monitored by ELISA Coated G4–6 was reacted with the biotinylated G2 domain in the presence of 1 m M EGTA (d) or in the presence of 1 m M CaCl 2 (s) at the concentrations indicated Binding was monitored at 405 nm using alkaline phosphatase labeled streptavidin Percentage binding was plotted vs G2 concentrations.
Trang 5show that the two peptides interacted with G4–6 in the
presence of EGTA In contrast, only the 153–193 peptide
interacted in the presence of calcium (Table 1) These results
were confirmed in solution For this aim, FITC labeled
G4–6 was mixed with each peptide supplemented with
EGTA or calcium In the presence of EGTA, changes in the
fluorescence intensity (Fig 7A,B) were obtained with the two fragments A maximum fluorescence quenching of 4% and fluorescence enhancement of 4% were calculated for 159–193 and 203–225 peptides, respectively When calcium
is present in the medium, binding of peptide 153–193 to labeled G4–6 induces an important decrease in fluorescence intensity (15%) (Fig 7) The last result demonstrates that the binding of the latter peptide causes a somewhat different conformational change in G4–6 domain In contrast no effect is observed for the 203–225 fragment, accordingly to ELISA experiments
Discussion
Since its discovery as an actin-depolymerizing factor, gelsolin has now been implicated in a number of important pathways such as apoptosis, oncogenic transformation, signal trans-duction and amyloidosis (reviewed [2]) All of these pathways are likely to involve calcium activation, the process
by which various binding sites become (especially actin) available for interaction In this paper we confirm that calcium causes a large conformational change in the C-terminal half of gelsolin (G4–6) that can be monitored
in a number of different techniques We also show that G2 undergoes a conformational change upon binding calcium
by a site that has a slightly higher affinity than was previously assumed This study also provides some details on the interface between these two gelsolin domains (G2 with G4–6) whose dissociation is pivotal in the activation of gelsolin
Calcium-dependent conformational changes in G4–6 That the C-terminal half of gelsolin (G4–6) binds calcium, leading to the activation of the whole gelsolin molecule
Fig 5 Binding of gelsolin G2 domain with G4–6 monitored by
fluor-escence measurements (A) Interaction of FITC labeled G2 domain
(0.2 l M ) with G4–6 was carried out in 0.1 M KCl 20 m M Tris buffer
pH 7.4 Change in fluorescence emission spectra of FITC was recorded
at various G4–6 concentrations (0–2.1 l M ) in the presence of 1 m M
EGTA (d) or 1 m M CaCl 2 (s) (B) Binding of G2 domain with FITC
labeled G4–6 (0.3 l M ) determined by fluorescence The experiment
was carried out in 0.1 M KCl, 20 m M Tris buffer pH 7.4 supplemented
with 1 m M EGTA (d) or 1 m M CaCl 2 (s).
Fig 6 Effect of calcium on the fluorescence of the FITClabeled G4–6/ G2 complex Maximum fluorescence enhancement (% initial fluores-cence) extrapolated to infinite G2 concentration is plotted vs free calcium concentration (pCa) Inset, apparent K d s for G2 Interactions with FITC labeled G4–6 are plotted vs pCa.
Table 1 Binding of G2 and derived peptide to G4–6.
EGTA K d (l M ) Calcium K d (l M )
Fluorescence ELISA Fluorescence ELISA
203–225 3 3 None None
Trang 6through large structural changes is well established [8,9] We
have shown that a calcium sensitive proteolysis between G5
and G6 occurs in agreement with the structural data [3,15]
that shows a long random coil connecting G5 to G6
Previous data from biochemical studies on G4–6 have
detect two calcium binding site one within G4-5
(Kd¼ 2 lM), the other within G5–6 (Kd¼ 0.2 lM) [7]
New data (Koloppan et al 2003) collected with actin-free
G4–6 identify calcium bound to G5 and G6 only, a finding
in agreement with Choe et al [3] who hypothesized that a
third site, IIG4, became coordinated solely by G4 residues
only when actin was bound (were this to be the case then
IIG4 is a site with properties between a site I and a site II
type) Taken together it seems that two sites are bound by
G4–6 in the absence of actin, one of these, IIG5 has a
moderate affinity (Kd¼ 2 lM) in agreement of this study
and previous work [7], the other IIG6 has a higher affinity
The slight difference observed between the present study
(Kd¼ 0.1 lM) and the previous study [7] who measured a
Kd of 0.2 lM, may be attributable to conformational
differences in the site in the context of G4–6 compared to
G5–6 or a degree of cocooperativity between calcium site
(see below)
A calcium-dependent conformational change in G2
A number of studies have concluded that calcium induces
conformational changes within G2 [11,36] A calcium
binding site has been detected in G1–3 [12], and others
[3,11] have located this site within G2 itself However, this
previous [11] study estimated a low affinity (Kd¼ 32 lM)
whereas we have estimated a slightly higher affinity
(Kd¼ 15 lM) We have also found a calcium-dependent
conformational change in G2 this may explain why the
reactivity of the thiol groups within G2 are calcium-sensitive
[37,38]
The G2 G4–6 interface
As expected we have found that G2 binds to G4–6 with high affinity (Kd¼ 0.5–0.8 lM) in EGTA, weaker binding was evident in the presence of 1 mM calcium (Fig 5) In addition a change in the interface occurs during calcium binding to IIG6 site (Fig 6) The interaction between gelsolin C-terminal helix (residues 744–755) and the helix of G2 is well known We have confirmed this interaction biochemically and found a calcium dependent interaction between G4–6 and the peptide 203–225 derived from G2 The calcium sensitivity of this interaction is probably due to IIG6 as the coordinating residues that comprise the IIG2 site are not all present within the peptide In addition to this expected interaction, we have also detected binding of G4–6
to a second G2-derived peptide 159–193 This interaction
is only marginally calcium sensitive and of higher affinity (Table 1) The EGTA structure [22] reveals that this peptide makes salt bridge contacts (R168–D669 and R169–D670) and that hydrophobic interactions also occur such as those between V170-V657, with G6 In summary, G2 binds to G4–6 through two distinct interfaces Binding site 1 involves G2 203–225 and G6 744–755 and binding site 2 involves G2 region around R168-R69 and G6 D669-D670
‘Unlatching’ and dissociation of the G2 and G6 connection
If the last 23 amino-acids are removed from gelsolin mutants, the requirement for calcium for actin-binding
is lessened but not abolished [39], similarly it has been determined that adseverin which naturally lacks the C-terminal helix has a similar calcium requirement than the helix minus gelsolin mutant [21] Together with recent observations on the structure of gelsolin [3,36], our new data on binding site 2 is compatible with the following
Fig 7 Effect of calcium on the interaction of two sequences involved in the G2–G4–6 interface monitored by fluorescence Binding of FITC labeled G4–6 (0.3 l M ) with two synthetic peptides derived from G2 domain: (A) sequence 203–225 and (B), sequence 159–193 The experiments were performed in 0.1 M KCl, 20 m M Tris buffer pH 7.4 supplemented with 1 m M EGTA (d) or 1 m M CaCl 2 (s).
Trang 7explanation It is likely that G2 remains held to G4–6
through binding site 2 in the absence of site 1
Occupancy of IIG2 has been proposed to disrupt site 1
[36], by disruption of hydrophobic and salt bridges between
G2 helix a1 and the C-terminal helix of G6 It is proposed
that binding of IIG6 disrupts the salt bridges between
D669 and R168, and between D670 and R169
Cooper-ativity between IIG2 and IIG6 has been suggested to occur
as a result of the breaking of site 1 and site 2, as breakage
of either connection frees up ligands to coordinate calciums
in either type II site [3] Calcium binding to whole gelsolin
has been found to be cooperative [40] it is possible that this
is due to coordination of calcium sites as is proposed for
IIG2 and IIG6, in addition to the general opening of the
molecule Cooperation of the sites would also explain why
the dissociation of G2 from G4–6 occurs at 100 nM
calcium (Fig 6), whereas we have measured a Kd value
of 15 lM, and others estimate 32 lM [11] for calcium
binding to IIG2 Occupancy of IIG6 may indirectly alter
IIG2 so that it too becomes a high affinity site We
measure the IIG6 Kdto be 100 nM (Fig 2) in agreement
with this model
Acknowledgements
This research was supported by grants from AFM.
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