Abbreviations CcO, cytochrome c oxidase; CcO4-2, CcO subunit 4-2 gene; Egr1, early growth response factor 1; EMSA, electrophoretic mobility shift assay; HIF-1a, hypoxia-inducible factor
Trang 1subunit IV-2 is controlled by a novel conserved oxygen
responsive element
Maik Hu¨ttemann, Icksoo Lee, Jenney Liu and Lawrence I Grossman
Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
Oxygen sensing and the adaptation to varying oxygen
concentrations are fundamental for the survival of
species from bacteria to humans Oxygen regulation
can occur at both the intake stage and the usage
stage In higher organisms, which depend largely on
aerobic energy metabolism, usage takes place largely
at the last step of the mitochondrial respiratory
chain, the transfer of electrons from cytochrome c to
oxygen, catalyzed by cytochrome c oxidase (CcO; EC
1.9.3.1)
In vertebrates, oxygen supply is also regulated via a unique mechanism in the lungs for hypoxic response Other tissues (and, indeed, the bronchial circulatory system of the lung) react to a hypoxic trigger by vaso-dilation, thereby increasing blood flow to under-oxy-genated regions In the pulmonary circulation of the lungs, however, the converse effect, hypoxic vasocon-striction, is critical for shunting blood to more highly ventilated regions to help optimize the ventilation of deoxygenated blood
Keywords
electron transport chain; hypoxia; isoform;
lung; mitochondria
Correspondence
L Grossman or M Hu¨ttemann, Molecular
Medicine and Genetics, Wayne State
University School of Medicine, 540 E.
Canfield Ave., Detroit, MI 48201, USA
Fax: + 1 313 5775218
Tel: + 1 313 5775326 or +1 3135779150
E-mail: l.grossman@wayne.edu,
mhuttema@med.wayne.edu
Database
CcO4-2 promoter sequences, including
exon I, have been submitted to the
GenBank data library under the accession
numbers: AY219183 (human), AY219183
(cow), AY219183 (rat) and AY219183
(mouse)
(Received 18 July 2007, revised 30 August
2007, accepted 5 September 2007)
doi:10.1111/j.1742-4658.2007.06093.x
Subunit 4 of cytochrome c oxidase (CcO) is a nuclear-encoded regulatory subunit of the terminal complex of the mitochondrial electron transport chain We have recently discovered an isoform of CcO 4 (CcO4-2) which is specific to lung and trachea, and is induced after birth The role of CcO as the major cellular oxygen consumer, and the lung-specific expression of CcO4-2, led us to investigate CcO4-2 gene regulation We cloned the CcO4-2promoter regions of cow, rat and mouse and compared them with the human promoter Promoter activity is localized within a 118-bp proxi-mal region of the human promoter and is stimulated by hypoxia, reaching
a maximum (threefold) under 4% oxygen compared with normoxia
CcO4-2oxygen responsiveness was assigned by mutagenesis to a novel promoter element (5¢-GGACGTTCCCACG-3¢) that lies within a 24-bp region that is 79% conserved in all four species This element is able to bind protein, and competition experiments revealed that, within the element, the four core bases 5¢-TCNCA-3¢ are obligatory for transcription factor binding CcO isolated from lung showed a 2.5-fold increased maximal turnover compared with liver CcO We propose that CcO4-2 expression in highly oxygenated lung and trachea protects these tissues from oxidative damage by accelerat-ing the last step in the electron transport chain, leadaccelerat-ing to a decrease in available electrons for free radical formation
Abbreviations
CcO, cytochrome c oxidase; CcO4-2, CcO subunit 4-2 gene; Egr1, early growth response factor 1; EMSA, electrophoretic mobility shift assay; HIF-1a, hypoxia-inducible factor 1a; ORE, oxygen responsive element; OREF, ORE binding factor; RACE, rapid amplification of cDNA ends; ROS, reactive oxygen species.
Trang 2At the gene level, a major breakthrough for the
understanding of oxygen sensing was the discovery
of hypoxia-inducible factor 1a (HIF-1a) [1], which,
during hypoxia, activates at least 60 genes involved
in energy metabolism, erythropoiesis and
vasculariza-tion It is clear, however, that HIF-1 is not adequate
to explain ischemic cellular pathophysiology For
example, ferrets develop profound pulmonary
hyper-tension when chronically exposed to oxygen
concen-trations of 10%, whereas, in ex vivo ferret lungs,
HIF-1a expression is not observed until oxygen
con-centration goes below 7%, rises somewhat at 4%
but still does not increase dramatically until 0–1.3%
[2] In addition, the deposition of fibrin in the lung
vasculature, which is a consequence of the induction
of procoagulant tissue factor, is independent of
HIF-1 [3], and has been shown to result from
hypoxia-mediated induction or activation of the
tran-scription factor early growth response factor 1 (Egr1)
[4] Our finding, reported here, that a new isoform
of CcO subunit 4 (CcO4-2), which is expressed in
lung and trachea [5], is regulated by oxygen
concen-tration adds a new component to the mechanism of
pulmonary oxygen sensing Furthermore, the recent
demonstration that HIF-1 stimulates CcO4-2
expres-sion under hypoxia [6], whilst down-regulating
mito-chondrial metabolism [7–9], underscores the role of
mitochondria in the hypoxic response The
mecha-nism for oxygen sensing in the lung is still
unre-solved Considerable data have suggested that the
sensor resides in the mitochondrial electron transport
chain [10–13], although contrary evidence has
recently been presented [14] Whilst this work was in
progress, Horvat et al [15] suggested that CcO4-2 is present in several mouse brain cell types under hypoxic conditions
We demonstrate in this report that CcO4-2 is oxy-gen-regulated at the transcriptional level It is induced under hypoxia via a conserved, novel, oxygen respon-sive element (ORE) in the proximal promoter that can be separated from the well-characterized HIF-1 system
Results
CcO4-2 is a respiratory system isoform Strong transcription signals of CcO4-2 in smooth mus-cle of the lung [5] led us to investigate transcript levels
in other nonlung smooth muscle tissues by quantitative PCR Compared with lung (100%), small intestines showed only trace amounts (< 0.5%), aorta 4.8% and trachea 100%, which extends CcO4-2 expression in the respiratory system but not to the other smooth muscle tissues examined
Phylogenetic footprinting utilizing ‘one-way PCR’ reveals a 24-bp conserved region in the CcO4-2 promoter
We cloned and sequenced the promoter region of cow, rat and mouse utilizing one-way PCR (see Experimen-tal procedures; Fig 1A) The alignment of the proxi-mal promoter sequences, including human, revealed low identities, except for a 24-bp region conserved in all four species (Fig 1B, black bar) Within the 400-bp
Fig 1 (A) Schematic representation of ‘one-way PCR’ This method is an extension of RACE PCR, and allows the rapid characterization of unknown upstream or downstream genomic DNA sequences (see Experimental procedures) A short sequence length must be known, e.g.
an exonic sequence derived from a cDNA Three subsequent primers directed to the region of interest are generated from the known sequence; here, the CcO4-2 promoter sequences of cow, mouse and rat were amplified The outermost primer P-1 is used to linearly amplify into the unknown region using genomic DNA as template The length-heterogeneous single-stranded fragments are polyadenylated.
In one reaction, a poly(T) primer, which contains an appended sequence (outer ⁄ inner) for later PCRs, is annealed to the poly(A) tails of the fragments, counter strand synthesis is performed as a single PCR cycle, followed by an outer PCR with the next inner primer P-2 and Qouter.
To increase specificity, a nested PCR is performed with primers P-3 and Qinnerand 1 lL of a 1 : 50 dilution of the outer PCR as template PCR products, separated by agarose gel electrophoresis, usually appear as a smear Fragments of the desired size are gel extracted, cloned and sequenced (B) Alignment of the CcO4-2 promoter sequences Proximal promoter sequences, including exon I from human, cow, rat and mouse, were aligned with the program MEGALIGN using the CLUSTAL algorithm Identical bases are indicated with an asterisk Transcription factor binding sites are underlined and specified for the human promoter Probes used for EMSA experiments are boxed Mutations used for control probes for EMSA and mutations introduced by site-directed mutagenesis for reporter gene analysis are indicated above the underlined elements in italics Exon I sequences are italicized The start ATG is located in exon II (not shown) The starting points of reporter gene constructs 4–7 in Fig 1C are indicated with arrows and fragment sizes A 24-bp region, conserved in all four species (black bar), is composed of a novel oxygen responsive element (ORE) and the adjacent Sp1 A site A HIF-1a element that has recently been suggested to regulate CcO4-2 [6] is underlined (C) Human CcO4-2 gene activity is driven by the ) 140-bp proximal promoter region Firefly luciferase reporter gene activity was normalized to cotransfected Renilla luciferase reporter gene activity, and relative reporter activities were standard-ized against the wild-type 579-bp construct reporter gene activity (set to 100%) after transfection and incubation under 20% oxygen for
40 h Control, reporter gene vector without inserted DNA (basal activity).
Trang 3promoter regions, including exon I, human and cow
share 52% and rat and mouse share 72% sequence
identity, whereas overall identities become insignificant
between the human⁄ cow and rat ⁄ mouse groups (26–
28%)
Human CcO4-2 gene activity is driven by the 118-bp proximal promoter region
Seven deletion fragments from the human CcO4-2 pro-moter were cloned in front of the luciferase gene, and
B
Trang 4reporter activity was tested in H460 human lung cells
(Fig 1C) Constructs starting from 2650 to 118 bp
revealed similar reporter activities, followed by an 80%
drop in activity obtained with the 76-bp construct We
concluded that the main regulatory elements are within
the)118-bp proximal region (see below)
A novel ORE mediates hypoxia-induced human
CcO4-2 gene activity
In order to investigate oxygen as a potential regulator
for CcO4-2 gene activity, we analyzed the 579- and
203-bp reporter constructs under both standard 20% oxygen cell culture conditions and at 2% oxygen Both reporter constructs showed more than twofold induc-tion under hypoxia (Fig 2A, constructs 1 and 2) Analysis of the human promoter with the program TFsearch [16] for potential transcription factor binding sites revealed three Sp1-like elements (Sp1A,B,C), one of which is part of the 24-bp conserved region (Fig 1B) The three elements were altered by site-directed muta-genesis of the 579-bp promoter construct, and activity was evaluated at 2% and 20% oxygen These experi-ments revealed that each is necessary for maximal pro-moter activity, but none is abolished by hypoxic induction after mutation (Fig 2A, constructs 4, 5 and 6) An additional construct was generated that con-tained mutations in the conserved 24-bp region (ORE in Fig 1B) upstream of the Sp1A site Mutation of this element significantly reduced reporter activity and, importantly, also eliminated CcO4-2 hypoxic induction, assigning oxygen responsiveness to the new element (Fig 2A, construct 3) Double mutations (Fig 2A, con-structs 7 and 8) are approximately additive and, when ORE is one of them, show a loss of hypoxic stimulation
CcO4-2 hypoxic response is threefold induced
at 4% oxygen
We analyzed the CcO4-2 hypoxic response in H460 cells between zero (set to 100%) and 20% oxygen
4% Oxygen
300
250
200
150
100
50
0
A
B
C
Fig 2 (A) Human CcO4-2 promoter activity is stimulated by hypoxia and mediated by a novel oxygen responsive element (ORE) Wild-type and site-directed mutagenesis constructs of the human
CcO4-2 promoter were transfected into human H460 cells that were grown under 20% (hatched bars) or 2% (filled bars) oxygen, and reporter gene activity was determined as in Fig 1 A 2.2-fold induc-tion in promoter activity was observed for the 579-bp wild-type reporter (construct 1) Hypoxic stimulation is abolished when ORE is mutated (black rectangle, construct 3) All indicated elements contrib-ute to maximum promoter activity (open circles, Sp1-like) (B) CcO4-2 reporter gene activity is about threefold increased at 4% oxygen compared with normoxia, whereas the HIF-1a construct shows highest activity under anoxia The reporter gene activity of the
579-bp human CcO4-2 (see Fig 1C) and the HIF-1a response element (HRE) reporter constructs was examined in H460 cells under various oxygen concentrations, and standardized against identically treated cells incubated at 20% oxygen in parallel (C) The 17-bp ORE was cloned in a promoterless reporter gene vector A one- (left) and four-(middle) copy-containing construct with the correctly oriented sequence and the control plasmid (right) were transfected into H460 cells and incubated under 4% and 20% oxygen concentra-tions, revealing that the ORE by itself is able to both stimulate tran-scription and mediate the hypoxic response Reporter activity for the one-copy-containing construct was 8.9% (± 0.5%) relative to the 579-bp construct (construct 1 in Fig 2A) at 20% oxygen.
Trang 5Compared with normoxia, reporter gene activity was
induced threefold at 4% oxygen, declining to about
1.7-fold induced at 0% (Fig 2B, filled diamonds) To
compare the CcO4-2 response with that of HIF-1, a
reporter containing three HIF-1a regulatory elements
was also introduced under the same conditions
(Fig 2B, open squares)
In both cases, the reporter assay was performed after
40 h of incubation at each indicated oxygen
concentra-tion To determine the time delay necessary for 1.1 mL
of fresh oxygen-saturated medium overlying the cells in
a 24-well plate to equilibrate with the gas atmosphere,
we positioned an oxygen electrode into a standard well
and recorded media oxygen concentration over time
(data not shown) Constant oxygen equilibrium
concen-trations were obtained after about 3 h for 20% and 4%
oxygen atmospheres, but not until more than 8 h for
0% oxygen Thus, the incubation period of 40 h prior
to reporter gene analysis allowed > 24 h exposure to
the experimental oxygen concentration
We next cloned ORE in a promoterless vector to
test whether its presence is sufficient to mediate a
hyp-oxic response in the absence of other cis elements in
the human promoter A construct containing one copy
of ORE produced a 2.8-fold induction of reporter gene
activity at 4% oxygen compared with normoxia
(Fig 2C, left columns) However, the presence of three
additional copies of the element in the same
orienta-tion did not further increase the hypoxic response
(Fig 2C, second column pair) A construct containing
mutations in ORE produced background reporter gene
activity at both 4% and 20% oxygen, similar to the
empty vector (Fig 2C, right two column pairs) Thus,
the element alone can act as a promoter and can
medi-ate the hypoxic response
Electrophoretic mobility shift assay (EMSA) with
ORE probe
The analysis of protein–DNA interaction for ORE was
performed with a probe containing part of the adjacent
Sp1A site and nuclear extract derived from H460 cells
grown under 2% oxygen Two complexes were formed
(Fig 3A, lane 2) that could be competed with
unla-beled probe (lane 3) The upper band was shown to be
a nonspecific artifact that could be competed with any
oligonucleotide (Fig 3D) Competition with the probe,
but with ORE mutated again, competed the
nonspe-cific upper band (Fig 3A, lane 4, triangle) but not
ORE with its binding factor (OREF) in the lower band
(arrow) Antibodies against Sp3, Sp4, HIF-1a and
CREB protein, to examine whether the cognate protein
was part of the shifted band complex, gave negative
results in all cases (Fig 3A, lanes 8–12) Antibodies against transcription factors c-Rel and Ikaros, which were suggested by Genomatix software as candidates for binding to this sequence, also did not produce a supershift or interference with binding (not shown) Furthermore, neither the use of nuclear extract derived from H460 cells grown under 20% instead of 2% oxy-gen (not shown), nor the addition of potential regula-tory nucleotides ATP, ADP (not shown), NAD+ and NADH (Fig 3A, lanes 13–15), affected the relative intensity of the specific band
A partial characterization of the 5¢-upstream bases required for the interaction of OREF with ORE (Fig 3B, lane 2) expands the core sequence identified
by site-directed mutagenesis (Fig 2A) We conclude from the above experiments and phylogenetic foot-printing (Fig 1B) that the sequence 5¢-GGA(C ⁄ T) GTTCCCACGT-3¢ represents the minimum OREF recognition sequence To further narrow the core bind-ing site, we performed experiments with competitor oligonucleotides for each position of the 15-bp region: for each nucleotide, a mixture of three competitors was generated containing the three bases not present
in the human sequence By applying a large excess (100-fold) of competitor mixture, only those reactions
in which the particular base is absolutely required for protein binding will produce a shifted band (Fig 3C, upper panel) Applying this method, we identified four essential bases 5¢-TCCCA-3¢ in the middle of the ORE sequence (Fig 3C, boxed) A reduction in stringency
by decreasing the amount of competitor DNA to a 10-fold excess revealed the participation of other bases with different signal intensities, and two bases (italicized) that do not seem to be required for factor binding (Fig 3C, lower panel) Thus, the final consen-sus sequence from the above data is 5¢-GGA(C ⁄ T) NTTCNCACG(C⁄ T)
Lung CcO is a high-activity isozyme
We isolated CcO from cow lung for the first time to our knowledge and also from cow liver, in both cases following our previous protocol [17] Both isoforms of CcO subunit IV are expressed in lung However, we obtained only a single band in the size range of sub-unit IV (Fig 4A), most probably because of the very similar sizes of both isoforms We then performed activity measurements Functional differences between the lung and liver isozymes became obvious after kinetic analysis CcO activity (turnover number) was measured by the polarographic method (Fig 4B) Lung CcO is more than 2.5 times as active at maximal turnover than liver CcO
Trang 6Our discovery of a new mammalian CcO isoform for
regulatory subunit 4 [5], whose expression is specific to
lung and trachea amongst the tissues examined, which
is induced after birth, and whose transcription is
dependent on oxygen concentration, points to a role
for CcO in respiratory physiology Strong expression within lung has been localized to smooth muscle, in both arteriole and bronchiole walls Each of these locations may involve a different functional role and thus, consequently, a different regulatory circuitry In bronchiole walls, our previous observation of CcO inhibition by the anti-asthma drug theophylline,
A
B
C
D
Trang 7followed by decreased ATP levels [17], suggests a role
in airway constriction
In arteriole walls, muscle can regulate blood flow in
response to hypoxic signals to ameliorate potential
ischemic damage In most cases, hypoxia triggers
vaso-dilation in order to increase blood flow to
under-oxy-genated tissue regions Uniquely, in the pulmonary
arterioles of the lung, however, hypoxia triggers
vaso-constriction in order to shunt blood to better ventilated
(apical) regions to improve the ventilation⁄ perfusion
ratio As alveolar sacs do not contain a muscle
compo-nent, hypoxic signals must be transmitted upstream to
the terminal bronchioles The nature of the initial
hyp-oxic signal is unresolved, although considerable data tie
it to the mitochondrial electron transport chain and,
in particular, to the free radicals generated there
[12,18,19] Two general factors are known to increase
free radical production in the mitochondria, the oxygen
concentration and the redox state of the electron
trans-port chain, because both oxygen and electrons are
required substrates for radical formation Lung cells
face the highest (atmospheric) oxygen concentrations compared with other tissues Therefore, special protec-tive adaptations would be expected that prevent excess free radical formation One way to prevent electron build-up in the mitochondria would be to increase CcO activity, which has been shown to be the rate-limiting step in the electron transport chain under physiological conditions [20,21] We have shown here that isolated CcO from lung is 2.5 times more active than liver CcO, and recent experiments with cells overexpressing CcO4-2have shown that CcO4-2 is superior to CcO4-1
at dissipating H2O2 build-up [6] Taken together, the expression of CcO4-2 in the highly oxygenated tissues
of lung and trachea, the increased activity of the iso-lated enzyme and the finding that CcO4-2-expressing cells produce fewer free radicals suggest a role in pro-tecting these tissues from radical damage Interestingly, CcO4-2 evolved by gene duplication about 320 million years ago [5], a time when atmospheric oxygen concen-trations dramatically increased from an estimated hyp-oxic level of 13% in the Devonian to 35% hyperoxia
Fig 3 (A) EMSA with the [ 32 P]-labeled oxygen responsive element (ORE) probe yields two specific bands The19-bp [ 32 P]-labeled ORE probe (bottom) contains four G nucleotides of the adjacent Sp1 site The nuclear extract was prepared from H460 lung cancer cells grown under 2% (+) oxygen for 2 days In each binding reaction, 60 lg of nuclear extract was applied The two strongly shifted bands (lane 2) can be removed with a 50-fold excess of unlabeled competitor DNA (lane 3) Competitor DNA that contains mutations in ORE (indicated on the top
of the probe sequence; lane 4) or competition experiments with an Sp1 fragment derived from the middle Sp1 site of the human promoter (Fig 1B) in wild-type (lane 5) or mutated (lane 6) form largely eliminates the upper band (triangle), and thus reveals specific interaction of ORE with a corresponding transcription factor (arrow) Supershift experiments with Sp antibodies show interference only with an Sp1 anti-body (lane 8) Normal goat serum (N) and Sp3, Sp4, HIF-1a and CREB binding protein antibodies show no effect (lanes 7, 9–12) Similarly, the addition of 2 m M NAD + (lane 14) or NADH (lane 15) shows no effect on the lower band Free probe at the bottom of each lane is not shown (B) EMSA experiments performed with probes differing in sequence length revealed that, in addition to the core sequence as shown above the 5¢-upstream bases, GGA is necessary for transcription factor binding, because the lower band representing OREF–ORE interaction
is abolished on its removal (lane 2) Probe sequences are indicated Stars show bases conserved in the human, cow, rat and mouse promot-ers (C) The definition of bases indispensable for transcription factor binding to ORE was performed via competition experiments using 15 oligonucleotides containing mutations at each position of the binding site as defined in (B) (lanes 2–16) Each competitor consists of a mix-ture of three oligonucleotides containing the bases not present in the ORE sequence (H, C ⁄ A ⁄ T; B, T ⁄ C ⁄ G; V, A ⁄ C ⁄ G; D, G ⁄ A ⁄ T) For exam-ple, the competitor in lane 2 contains three double-stranded oligonucleotides with C, A and T in the first position, excluding G present in the wild-type ORE EMSA was performed under the conditions given in (A) using a 10- or 100-fold excess of unlabeled competitor as indicated Only the lower band specific for OREF binding is shown (see (A), arrow) The absence of competitor DNA produces a similar signal, as observed with unspecific competitor under 10- or 100-fold excess (compare lanes 1 and 17; see Table 1 for oligonucleotide sequences) Using a 100-fold excess of competitor DNA reveals that four bases (boxed) are indispensable for OREF binding (lanes 8, 9, 11 and 12), because the corresponding competitor DNAs cannot compete with complex formation Reducing the stringency by applying a 10-fold excess
of competitor reveals signals with varying intensities for most positions (lanes 2–5, 7–9, 11–16), except for two bases (italicized) that do not seem to contribute to OREF binding (lanes 6 and 10) (D) EMSA experiments with ORE and Sp1 probes reveal that the upper band is non-specific for Sp1 The higher molecular weight complex could be competed with an unlabeled Sp1 B probe or the addition of Sp1 antibody [see (A), triangle; lanes 5 and 8, respectively] Thus, in order to test whether the upper band is specific and contains Sp1, side-by-side com-petition experiments were performed using the ORE probe and an Sp1 consensus probe identical in length and GC ⁄ AT content (Table 1) with nuclear extract from H460 cells grown at 20% oxygen In comparison with nuclear extract grown under hypoxia, normoxic nuclear extract leads to an increase in the upper band, but does not affect the intensity of the lower (specific) band The Sp1 probe shows three bands, with the lowest band migrating at the position of the upper band obtained with the ORE probe (triangle; compare lanes 9 and 2, respectively) Using both probes, this band could be competed with wild-type and mutated Sp1 oligonucleotides (lanes 3, 4, 10, 11) In addi-tion, a mixture of unspecific oligonucleotides that do not contain Sp1-like sequences (see Table 1) efficiently abolishes the band already at low excess (lanes 6, 7, 13 and 14), indicating that the upper band obtained with ORE and the lowest band obtained with the Sp1 probe result from unspecific (sequence-independent) protein binding This band is weakened on addition of Sp1 antibody (lanes 5 and 12); how-ever, using the Sp1 probe only, the top band (arrow) produces a specific supershift (star; compare lanes 9 and 12).
Trang 8during the Permian and Carboniferous, compared with
the present 21% Compared with other CcO subunit
isoforms, mammalian CcO subunit 4 led to the
genera-tion of the earliest isoform pair during a period with
dramatic changes in oxygen concentration, the
sub-strate of CcO, which further suggests an adaptation to
higher oxygen concentrations
A recent report has suggested that CcO4-2
regula-tion is mediated by HIF-1a [6], and the authors
identi-fied two such elements, one in the promoter (Fig 2, double underline) and one in intron 1 The involve-ment of HIF-1a or HIF-2a in CcO4-2 regulation needs further evaluation because both elements are not conserved in mammals, and it remains to be shown whether, under physiological conditions, lung cells face such low oxygen concentrations under which HIF reg-ulation is operating (Fig 2B) We found maximal CcO4-2 reporter gene activity at 4% oxygen, condi-tions under which regulation by HIF does not occur (Fig 2B) Oxygen concentration in lung is clearly higher than that in other tissues, and 4% oxygen, as applied during our cell culture experiments, might rep-resent the physiological equivalent range prep-resent at the cellular level in lung tissue
The properties of CcO4-2 in withstanding oxidative stress (faster ability to utilize O2 and produce ATP, whilst producing less H2O2 and caspase activation) suggest that its expression may be found in other cell types where survival is critical The question then arises as to why CcO4-2 has not become the dominant, tissue-unspecific isoform The answer may be that it is not very conservative of energy because of its increased basal activity (Fig 4B) Mitochondrial reactive oxygen species (ROS) have been shown to trigger hypoxia-stimulated responses, including transcription and cal-cium increases in pulmonary arterial myocytes [18,22] Inhibitor studies to localize the ROS-producing seg-ment of the electron transport chain place it proximal
to the ubisemiquinone site of complex III [11] How-ever, inhibitors acting distal to ubisemiquinone, such
as the CcO inhibitors cyanide and azide, can augment ROS generation by increasing the ubisemiquinone pool [10] As discussed above, regulation of electron flux at CcO by subunit 4-2, in analogy with ATP regulation
of CcO activity by subunit 4-1 [23], would be a way of modulating the redox state of the ubiquinone pool Our results stimulate the determination of how oxy-gen concentration regulates CcO4-2 expression The discovery of a novel 24-bp region in the proximal pro-moter, conserved between human, cow, rat and mouse, containing an element (ORE) shown by mutagenesis to
be required for oxygen regulation, leads to the ques-tion of what factor binds to this element and how it mediates this response The use of nuclear extract obtained from H460 cells grown under normoxia or hypoxia did not show differences in ORE–OREF bind-ing, indicating that the amount of OREF does not change as a function of the oxygen concentration Pos-sibly, the interaction of OREF with other factors is modified as a function of the oxygen concentration If OREF is not the oxygen sensor, but a downstream factor of the actual oxygen sensor, OREF could be
A
B
Fig 4 (A) SDS-PAGE of isolated CcO from cow lung in comparison
with liver CcO CcO samples from liver and lung were isolated
side-by-side and applied to SDS-PAGE Lane M, molecular size
mar-ker; lanes 1 and 2, 37% and 45% ammonium sulfate-precipitated
lung CcO; lane 3, 45% ammonium sulfate-precipitated cow liver
CcO (B) Respiration kinetics of solubilized cow lung CcO in
com-parison with liver CcO CcO activity was measured with the
polaro-graphic method at 25 C by increasing the amount of substrate
cytochrome c CcO activity (TN, turnover number) is defined as the
amount of O2 consumed (lmol) per second per amount of CcO
(lmol) The data shown were obtained with the 45% ammonium
sulfate-precipitated fractions CcO activity was analyzed in a closed
200 lL chamber containing a micro-Clark-type oxygen electrode
(Oxygraph system, Hansatech) Representative data from a total of
four independent experiments are shown.
Trang 9targeted for phosphorylation, altering its interaction
with other factors involved in complex formation As
several Sp1-like sites are present in the CcO4-2
pro-moter, indirect regulation is possible, involving
modifi-cations that modulate the potential OREF–Sp1
interaction, such as Sp1 phosphorylation [24]
In lower organisms there are two examples of
oxygen-mediated CcO isoform gene expression In yeast, there
are isoforms of subunit 5, called 5a and 5b, which are
expressed under normoxia and hypoxia, respectively,
and have been proposed to be analogous to
mamma-lian CcO subunit 4 [25] However, mammamamma-lian
CcO4-1⁄ 2 and yeast CcO5a ⁄ b do not share any homology at
the protein level In the slime mold Dictyostelium
dis-coideum, there is an isoform pair of CcO subunit 7
Here, CcO7e, which is expressed under normoxic
con-ditions, is replaced by CcO7s under hypoxia, and this
switching is mediated by an oxygen-dependent
tran-scriptional element located in the short intergenic
region between the two adjacent genes [26] Analysis of
the yeast and Dictyostelium CcO hypoxia-regulated
promoters revealed that the mammalian ORE sequence
is absent in both organisms, which agrees with our
functional model that the expression of CcO4-2
repre-sents a unique protective adaptation found in the
highly oxygenated respiratory system in higher
organ-isms, rather than being an adaptation to very low
oxy-gen levels, under which yeast CcO5b and Dictyostelium
CcO7s are expressed
Experimental procedures
Cell lines and reagents
Human lung adenocarcinoma-derived cell line H460 was
grown in RMPI 1640 medium (Gibco BRL, Carlsbad, CA,
USA) containing 0.1% glucose supplemented with 10%
fetal bovine serum and 1% penicillin–streptomycin in a 5%
CO2atmosphere Parallel experiments involving varying O2
concentrations were performed in a hypoxic chamber under
the control of ProOx 110 oxygen and ProCO2 carbon
dioxide controllers (BioSperix, Redfield, NY, USA)
Media were supplemented with 50 mgÆmL)1 uridine and
110 mgÆmL)1 pyruvate in experiments performed at 0%
oxygen [27] HeLa cells were grown in DMEM (Gibco
BRL) containing 0.1% glucose supplemented with 10%
fetal bovine serum and 1% penicillin–streptomycin
RNA isolation and quantitative PCR
Lung, heart, small intestine, aorta and trachea RNA
isola-tions and quantitative PCR with specific primers for
CcO4-2 and CcO4-1 were performed as described previously [5]
Cloning of the promoter regions with a novel method: ‘one-way PCR’ (Fig 1A)
In order to amplify the unknown CcO4-2 5¢-genomic pro-moter region from cow, mouse and rat, we developed a novel method, which is an extension of 5¢-rapid amplifica-tion of cDNA ends (5¢-RACE) used to generate 5¢-cDNAs [28] This approach utilizes a dT17-oligonucleotide (QT pri-mer) which contains an appended sequence that allows the use of specific primers (Qinner and Qouter) in subsequent PCRs Genomic DNA, isolated from muscle tissue of all species using the Wizard Genomic DNA Isolation Kit (Pro-mega, Madison, WI, USA), was used as template instead of RNA in the RACE protocol Three primers directed to the unknown 5¢-region (P-1cow, P-2cow, P-3cow, P-1rat, P-2rat, P-3rat, P-1mouse, P-2mouse and P-3mouse) were derived from known cDNA exon I (cow and rat) or intron I (mouse) sequences [5] In a first linear PCR amplification, the outer-most primer P-1 was used without a counter primer in a
50 lL PCR for each species employing the Expand Long Template PCR System (Roche, Indianapolis, IN, USA) in combination with the kit’s buffer 3 and a 0.5 mm final con-centration of each dNTP Initial denaturation at 93C for
2 min was followed by 5 s at 93C, 30 s at 65 C and
2 min at 68C, with 30 cycles in total Buffer components and dNTPs were removed from the mix, a poly(A) tail was appended to the single-stranded DNA (equivalent to the cDNA first strands in the 5¢-RACE method) and 5 lL (of
25 lL) of the previous reaction was used to anneal the QT
primer and for the extension reaction, as described previ-ously [29] Primers P-2 and Qouter(15 pm each) were added
to the mixture, and the outer PCR was performed with an initial denaturation at 93C for 1 min, followed by 30 s at
93C, 30 s at 58 C and 2 min at 72 C, with 30 cycles in total A 50 lL nested PCR was then carried out with prim-ers P-3 and Qinner, and 1 lL of a 1 : 30 dilution of the pre-vious reaction as template, using similar conditions as in the outer PCR (summarized in Fig 1A) The amplifications yielded a smeary size distribution on agarose gel electro-phoresis, ranging from 500 bp to 3 kb, because of the absence of a counter primer in the initial linear amplifica-tion reacamplifica-tion DNA between 1 and 2 kb was cut out of the gel and purified using the Nucleotrap Gel Extraction Kit (Clontech, Mountain View, CA, USA) DNA fragments were cloned and sequenced as described previously [5]
Reporter gene constructs
A 2.8-kb genomic fragment of the human CcO4-2 promoter, including exon I, was amplified from human genomic DNA with primers Ppromoter-forward and Ppromoter-reversein a 50 lL touchdown PCR () 1 C ⁄ cycle), with denaturation for 35 s
at 94C, annealing for 30 s at 63–58 C, extension for
4 min at 70C, and 32 cycles in total, using the Expand
Trang 10High Fidelity PCR System (Roche) Seven different-sized
promoter fragments (2646, 579, 399, 293, 203, 118, 76 bp,
all including 56 bp of exon I) were generated in separate
nested PCRs using 1 lL of a 1 : 40 dilution of the previous
PCR as template and primers Pprom+1,2,3,4,5,6,7 in
combi-nation with Ppromreverseunder similar touchdown conditions
All Ppromprimers contained a XhoI 5¢-adapter sequence
AG-TCTATTCTCGAG (Table 1) Fragments were gel-purified
(see above), digested with XhoI (Promega) and gel-purified
once more The longest fragment was only partially digested
as it contained an internal XhoI site The pGL3basic
lucifer-ase reporter vector (Promega) was digested with XhoI,
dephosphorylated with shrimp alkaline phosphatase (Roche)
and used for ligation of the seven promoter fragments
Cor-rect orientation of individual clones was tested by PCR, and
positive clones were verified by sequencing
The HIF-1a construct was a kind gift from Dr Navdeep
Chandel (Northwestern University, Evanston, IL, USA) It
contains three copies of the 5¢-RCGTG-3¢ motif in front
of the luciferase gene in the pGL2 reporter vector (Promega)
A promoterless reporter gene vector was generated by removing the SV40 promoter from the pGL2 Promoter vec-tor (Promega) via a HindIII⁄ XhoI double digestion The vector fragment lacking the SV40 promoter was purified as above, DNA ends were filled using Pfu polymerase (Strata-gene, La Jolla, CA, USA) and the vector was treated with shrimp alkaline phosphatase (Roche) The ORE-containing sequence 5¢-GGACGTTCCCACGCTGG-3¢ and the mutated sequence 5¢-GGTCGTAACCACGCTGG-3¢ were cloned into the vector in various configurations and confirmed by sequencing
Site-directed mutagenesis The 579-bp promoter construct was used for the generation
of all further constructs Primers PORE mut, PSp1 distal mut,
PSp1 middle mutand PSp1 proximal mut(Table 1) were used for site-directed mutagenesis with the GeneEditor site-directed mutagenesis kit (Promega), according to the supplier’s pro-tocol
Transfection and luciferase assay H460 cells were plated onto 24-well plates at 4· 104cells⁄ well and grown overnight Cells were transfected using TransFast (Promega) with 1 lg of the promoter firefly lucif-erase construct and 0.04 lg of the pRL-SV40 control vector (Promega), which contains the Renilla luciferase cDNA downstream of the SV40 promoter Cells were harvested
40 h after transfection and both luciferase activities were analyzed with the Dual-Luciferase Reporter Assay System (Promega), according to the supplier’s protocol, with an Optocomp 1 luminometer (MGM Instruments, Sparks, NV, USA) At least four replicates were performed for each
Preparation of nuclear extract and EMSA Nuclear protein extracts were prepared from H460 cells as described previously [30], HeLa nuclear extracts were pur-chased from Promega and protein concentrations were determined using the Bradford assay (Bio-Rad, Hercules,
CA, USA) The oligonucleotide primers PORE, PORE mut,
PSp1, PSp1 mut, PSp1 19bp, PSp1 19bp mutand Punspecific com-petitor, and the 15 primer mixes containing mutations in each position of the core ORE sequence (see Fig 3C), together with their reversed and complemented primers, were heated to 85C and slowly cooled to room tempera-ture in annealing buffer (10 mm MgCl2, 50 mm NaCl,
20 mm Tris⁄ Cl, pH 7.5) [c-32P]-labelling of double-stranded oligonucleotides, their purification and subsequent nuclear extract binding reactions were carried out as described previously [31] The DNA-bound complexes were
Table 1 Sequences of oligonucleotides used in RACE, ‘one-way
PCR’, site-directed mutagenesis and EMSA.
Primer ID Sequence (5’- to 3’)
P-1cow TCTTGCGGCTTGGAGAGAGCCAG
P-3cow CAGGTCTGCAGAGCAAGCAACAG
P-1rat TAGTTGCAAGCTGAAGACCG
P-2 rat GCTGAAGACCGCGGAGGTAC
P-3rat GAGGTACCCAGAACTGCCCTG
P-1mouse GATAGTCAGTGGGGGAAACCTCAG
P-2 mouse CAGCAAAAGAGGGCTGTGTGGTG
P-3mouse TGGCCGCCACGAACATCCCATC
Ppromoter forward GTTGCCCAGGTTGGAGTGCAG
P promoter reverse CTCGCGGGCTCGGCAGTGGGAG
P prom+1 AGTCTATTCTCGAGCACCTGGGACTACAGG
Pprom+2 AGTCTATTCTCGAGCCCAAAGCGCTGAGATTACAG
P prom+3 AGTCTATTCTCGAGATGCTTCTGGAGTAGGAGGCA
P prom+4 AGTCTATTCTCGAGGTGTGGAGGAGGCAGGGAGAC
Pprom+5 AGTCTATTCTCGAGGAGGCGCTCTGCAGTGCCTC
Pprom+6 AGTCTATTCTCGAGAAGCAGGACGTTCCCACGCTG
P prom+7 AGTCTATTCTCGAGGGGGCGGGCGCCCGCACTCAG
Ppromreverse AGTCTATTCTCGAGCGCGACCTGGGTCTGCCCAG
PORE mut GGCCGCCCCAGCGTGGTTACGACCTGCTTCGGCAGG
GCGTGG
P Sp1 distal mut GCCTTTCTCGGGGCCGCTTCAGCGTGGGAACG
PSp1 middle mut GGCGCCCGCCCCCGGCCATACCACAGCCTTTCTCGG
P Sp1 proximal mut GCGGGCGCCCGAACCCTGCCCGCCCCACA
P intron IIIfoward ATATTCTAGGATCCTGGCTCATTCACTGCTGTCAC
Pintron IIIreverse ATATTCTAGGATCCCGGCTTCCCCCTCCCTGCAG
P HIF-1a mut GCAAATTCTTACTGAGCTTTTACTATATGCACAGC
PORE mut GGTCGTAACCACGCTGGGG
P Sp1 mut GGCTGTGGGTATGGCCGG
PSp1 19bp TTCGATCGGGGCGGGGCGA
PSp1 19bp mut TTCGATCGGTTCGGGGCGA
P unspecific competitor CTAGCAANNATNNTTGCTAG